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Neptune throws the above error when trying to create a complex microfluidic device. I got this error with the following code. EnforceDRC was true and only two iterations were planned. Reducing to one iteration still throws this error. The error appears to occur during the "Running Cleanup" phase. I have pasted the log below and the MINT file. The error prevented me from routing the 8 channel initial design I needed:
CHANNEL channel_1 from primerin_0 2 to primerin_0_rtpcrmix 1 w=100;
CHANNEL channel_2 from primerin_1 2 to primerin_1_rtpcrmix 1 w=100;
CHANNEL channel_3 from primerin_2 2 to primerin_2_rtpcrmix 1 w=100;
CHANNEL channel_4 from primerin_3 2 to primerin_3_rtpcrmix 1 w=100;
CHANNEL channel_5 from primerin_4 2 to primerin_4_rtpcrmix 1 w=100;
CHANNEL channel_6 from primerin_5 2 to primerin_5_rtpcrmix 1 w=100;
CHANNEL channel_7 from primerin_6 2 to primerin_6_rtpcrmix 1 w=100;
CHANNEL channel_8 from primerin_7 2 to primerin_7_rtpcrmix 1 w=100;
CHANNEL channel_9 from pcrmix 2 to interaction_rtmix_pcrmix 1 w=1100;
CHANNEL channel_10 from rtmix 2 to interaction_rtmix 1 w=100;
CHANNEL channel_11 from rtpcrmix 2 to primerin_0_rtpcrmix 1 w=100;
CHANNEL channel_12 from rtpcrmix 2 to primerin_1_rtpcrmix 1 w=100;
CHANNEL channel_13 from rtpcrmix 2 to primerin_2_rtpcrmix 1 w=100;
CHANNEL channel_14 from rtpcrmix 2 to primerin_3_rtpcrmix 1 w=100;
CHANNEL channel_15 from rtpcrmix 2 to primerin_4_rtpcrmix 1 w=100;
CHANNEL channel_16 from rtpcrmix 2 to primerin_5_rtpcrmix 1 w=100;
CHANNEL channel_17 from rtpcrmix 2 to primerin_6_rtpcrmix 1 w=100;
CHANNEL channel_18 from rtpcrmix 2 to primerin_7_rtpcrmix 1 w=100;
CHANNEL channel_19 from rtpcrprimer_0 2 to rtpcrmixer_0 1;
CHANNEL channel_20 from rtpcrprimer_1 2 to rtpcrmixer_1 1;
CHANNEL channel_21 from rtpcrprimer_2 2 to rtpcrmixer_2 1;
CHANNEL channel_22 from rtpcrprimer_3 2 to rtpcrmixer_3 1;
CHANNEL channel_23 from rtpcrprimer_4 2 to rtpcrmixer_4 1;
CHANNEL channel_24 from rtpcrprimer_5 2 to rtpcrmixer_5 1;
CHANNEL channel_25 from rtpcrprimer_6 2 to rtpcrmixer_6 1;
CHANNEL channel_26 from rtpcrprimer_7 2 to rtpcrmixer_7 1;
CHANNEL channel_27 from outstage_0 2 to amplifiedout_0 1;
CHANNEL channel_28 from outstage_1 2 to amplifiedout_1 1;
CHANNEL channel_29 from outstage_2 2 to amplifiedout_2 1;
CHANNEL channel_30 from outstage_3 2 to amplifiedout_3 1;
CHANNEL channel_31 from outstage_4 2 to amplifiedout_4 1;
CHANNEL channel_32 from outstage_5 2 to amplifiedout_5 1;
CHANNEL channel_33 from outstage_6 2 to amplifiedout_6 1;
CHANNEL channel_34 from outstage_7 2 to amplifiedout_7 1;
CHANNEL channel_35 from rtpcrmixer_0 2 to rtpHeatChamber_0 1;
CHANNEL channel_36 from rtpcrmixer_1 2 to rtpHeatChamber_1 1;
CHANNEL channel_37 from rtpcrmixer_2 2 to rtpHeatChamber_2 1;
CHANNEL channel_38 from rtpcrmixer_3 2 to rtpHeatChamber_3 1;
CHANNEL channel_39 from rtpcrmixer_4 2 to rtpHeatChamber_4 1;
CHANNEL channel_40 from rtpcrmixer_5 2 to rtpHeatChamber_5 1;
CHANNEL channel_41 from rtpcrmixer_6 2 to rtpHeatChamber_6 1;
CHANNEL channel_42 from rtpcrmixer_7 2 to rtpHeatChamber_7 1;
CHANNEL channel_43 from rtpHeatChamber_0 2 to outstage_0 1;
CHANNEL channel_44 from rtpHeatChamber_1 2 to outstage_1 1;
CHANNEL channel_45 from rtpHeatChamber_2 2 to outstage_2 1;
CHANNEL channel_46 from rtpHeatChamber_3 2 to outstage_3 1;
CHANNEL channel_47 from rtpHeatChamber_4 2 to outstage_4 1;
CHANNEL channel_48 from rtpHeatChamber_5 2 to outstage_5 1;
CHANNEL channel_49 from rtpHeatChamber_6 2 to outstage_6 1;
CHANNEL channel_50 from rtpHeatChamber_7 2 to outstage_7 1;
CHANNEL channel_51 from interaction_rtmix 2 to interaction_rtmix_pcrmix 1;
CHANNEL channel_52 from interaction_rtmix_pcrmix 2 to rtpcrmix 1;
CHANNEL channel_53 from primerin_0_rtpcrmix 2 to rtpcrprimer_0 1;
CHANNEL channel_54 from primerin_1_rtpcrmix 2 to rtpcrprimer_1 1;
CHANNEL channel_55 from primerin_2_rtpcrmix 2 to rtpcrprimer_2 1;
CHANNEL channel_56 from primerin_3_rtpcrmix 2 to rtpcrprimer_3 1;
CHANNEL channel_57 from primerin_4_rtpcrmix 2 to rtpcrprimer_4 1;
CHANNEL channel_58 from primerin_5_rtpcrmix 2 to rtpcrprimer_5 1;
CHANNEL channel_59 from primerin_6_rtpcrmix 2 to rtpcrprimer_6 1;
CHANNEL channel_60 from primerin_7_rtpcrmix 2 to rtpcrprimer_7 1;
END LAYER
LAYER CONTROL
PORT p3 r=800;
VALVE v1 on channel_9 w=300 l=100;
NET net1 from p3 1 to v1 4 channelWidth=100;
END LAYER
The text was updated successfully, but these errors were encountered:
Neptune throws the above error when trying to create a complex microfluidic device. I got this error with the following code. EnforceDRC was true and only two iterations were planned. Reducing to one iteration still throws this error. The error appears to occur during the "Running Cleanup" phase. I have pasted the log below and the MINT file. The error prevented me from routing the 8 channel initial design I needed:
LOG--------------------------------------------------------------------------------------
Beginning Place and Route
PAR iteration 1
Initial Place
Initial Temp
Initial Temp = 2.0285066290925857E12
-> Temp = 1.0142533145462928E12
-> Temp = 5.071266572731464E11
-> Temp = 2.535633286365732E11
-> Temp = 1.267816643182866E11
-> Temp = 6.33908321591433E10
-> Temp = 5.705174894322897E10
-> Temp = 5.1346574048906075E10
-> Temp = 4.6211916644015465E10
-> Temp = 4.159072497961392E10
-> Temp = 3.7431652481652534E10
-> Temp = 3.368848723348728E10
-> Temp = 3.0319638510138554E10
-> Temp = 2.72876746591247E10
-> Temp = 2.455890719321223E10
-> Temp = 2.2103016473891006E10
-> Temp = 1.9892714826501907E10
-> Temp = 1.790344334385172E10
-> Temp = 1.6113099009466547E10
-> Temp = 1.4501789108519892E10
-> Temp = 1.3051610197667902E10
-> Temp = 1.1746449177901112E10
-> Temp = 1.0571804260111E10
-> Temp = 9.514623834099901E9
-> Temp = 8.563161450689911E9
-> Temp = 7.70684530562092E9
-> Temp = 6.936160775058828E9
-> Temp = 6.242544697552946E9
-> Temp = 5.618290227797651E9
-> Temp = 5.056461205017886E9
-> Temp = 4.550815084516098E9
-> Temp = 4.0957335760644884E9
-> Temp = 3.6861602184580398E9
-> Temp = 3.317544196612236E9
-> Temp = 2.9857897769510126E9
-> Temp = 2.6872107992559114E9
-> Temp = 2.4184897193303204E9
-> Temp = 2.1766407473972883E9
-> Temp = 1.9589766726575596E9
-> Temp = 1.8610278390246816E9
-> Temp = 1.7679764470734475E9
-> Temp = 1.679577624719775E9
-> Temp = 1.595598743483786E9
-> Temp = 1.5158188063095968E9
-> Temp = 1.4400278659941168E9
-> Temp = 1.3680264726944108E9
-> Temp = 1.2996251490596902E9
-> Temp = 1.2346438916067057E9
-> Temp = 1.1729116970263703E9
-> Temp = 1.1142661121750517E9
-> Temp = 1.0585528065662991E9
-> Temp = 1.0056251662379841E9
-> Temp = 9.553439079260848E8
-> Temp = 9.075767125297805E8
-> Temp = 8.621978769032915E8
-> Temp = 8.190879830581268E8
-> Temp = 7.781335839052204E8
-> Temp = 7.392269047099594E8
-> Temp = 7.022655594744614E8
-> Temp = 6.671522815007384E8
-> Temp = 6.337946674257014E8
-> Temp = 6.021049340544163E8
-> Temp = 5.719996873516954E8
-> Temp = 5.433997029841106E8
-> Temp = 5.16229717834905E8
-> Temp = 4.9041823194315976E8
-> Temp = 4.6589732034600174E8
-> Temp = 4.426024543287016E8
-> Temp = 4.2047233161226654E8
-> Temp = 3.9944871503165317E8
-> Temp = 3.794762792800705E8
-> Temp = 3.605024653160669E8
-> Temp = 3.424773420502636E8
-> Temp = 3.253534749477504E8
-> Temp = 3.0908580120036286E8
-> Temp = 2.936315111403447E8
-> Temp = 2.789499355833274E8
-> Temp = 2.6500243880416104E8
-> Temp = 2.5175231686395296E8
-> Temp = 2.391647010207553E8
-> Temp = 2.272064659697175E8
-> Temp = 2.1584614267123163E8
-> Temp = 2.0505383553767005E8
-> Temp = 1.9480114376078653E8
-> Temp = 1.850610865727472E8
-> Temp = 1.7580803224410984E8
-> Temp = 1.6701763063190433E8
-> Temp = 1.586667491003091E8
-> Temp = 1.5073341164529365E8
-> Temp = 1.4319674106302896E8
-> Temp = 1.360369040098775E8
-> Temp = 1.2923505880938362E8
-> Temp = 1.2277330586891443E8
-> Temp = 1.166346405754687E8
-> Temp = 1.1080290854669526E8
-> Temp = 1.0526276311936049E8
-> Temp = 9.999962496339247E7
-> Temp = 9.499964371522284E7
-> Temp = 9.02496615294617E7
-> Temp = 8.573717845298861E7
-> Temp = 8.145031953033918E7
-> Temp = 7.737780355382222E7
-> Temp = 7.35089133761311E7
-> Temp = 6.983346770732455E7
-> Temp = 6.634179432195832E7
-> Temp = 6.30247046058604E7
-> Temp = 5.987346937556738E7
-> Temp = 5.6879795906789005E7
-> Temp = 5.4035806111449555E7
-> Temp = 5.1334015805877075E7
-> Temp = 4.876731501558322E7
-> Temp = 4.632894926480406E7
-> Temp = 4.401250180156385E7
-> Temp = 4.1811876711485654E7
-> Temp = 3.972128287591137E7
-> Temp = 3.77352187321158E7
-> Temp = 3.584845779551001E7
-> Temp = 3.405603490573451E7
-> Temp = 3.2353233160447784E7
-> Temp = 3.0735571502425395E7
-> Temp = 2.9198792927304123E7
-> Temp = 2.7738853280938916E7
-> Temp = 2.635191061689197E7
-> Temp = 2.503431508604737E7
-> Temp = 2.3782599331745E7
-> Temp = 2.259346936515775E7
-> Temp = 2.146379589689986E7
-> Temp = 2.0390606102054868E7
-> Temp = 1.9371075796952125E7
-> Temp = 1.8402522007104516E7
-> Temp = 1.748239590674929E7
-> Temp = 1.6608276111411825E7
-> Temp = 1.5777862305841234E7
-> Temp = 1.498896919054917E7
-> Temp = 1.4239520731021712E7
-> Temp = 1.3527544694470625E7
-> Temp = 1.2851167459747093E7
-> Temp = 1.2208609086759737E7
-> Temp = 1.1598178632421749E7
-> Temp = 1.1018269700800661E7
-> Temp = 1.0467356215760628E7
-> Temp = 9943988.404972596
-> Temp = 9446788.984723967
-> Temp = 8974449.535487767
-> Temp = 8525727.058713378
-> Temp = 8099440.705777709
-> Temp = 7694468.670488823
-> Temp = 7309745.236964381
-> Temp = 6944257.975116162
-> Temp = 6597045.076360353
-> Temp = 6267192.822542335
-> Temp = 5953833.181415218
-> Temp = 5656141.522344457
-> Temp = 5373334.446227234
-> Temp = 5104667.723915872
-> Temp = 4849434.337720078
-> Temp = 4606962.620834074
-> Temp = 4376614.48979237
-> Temp = 4157783.7653027517
-> Temp = 3949894.577037614
-> Temp = 3752399.848185733
-> Temp = 3564779.855776446
-> Temp = 3386540.8629876235
-> Temp = 3217213.819838242
-> Temp = 3056353.12884633
-> Temp = 2903535.4724040134
-> Temp = 2758358.6987838126
-> Temp = 2620440.763844622
-> Temp = 2489418.7256523906
-> Temp = 2364947.789369771
-> Temp = 2246700.399901282
-> Temp = 2134365.379906218
-> Temp = 2027647.110910907
-> Temp = 1926264.7553653615
-> Temp = 1829951.5175970932
-> Temp = 1738453.9417172384
-> Temp = 1651531.2446313764
-> Temp = 1568954.6823998075
-> Temp = 1490506.9482798171
-> Temp = 1415981.6008658262
-> Temp = 1345182.5208225348
-> Temp = 1277923.394781408
-> Temp = 1214027.2250423376
-> Temp = 1153325.8637902206
-> Temp = 1095659.5706007094
-> Temp = 1040876.5920706739
-> Temp = 988832.7624671402
-> Temp = 939391.1243437831
-> Temp = 892421.568126594
-> Temp = 847800.4897202642
-> Temp = 805410.465234251
-> Temp = 765139.9419725384
-> Temp = 726882.9448739114
-> Temp = 690538.7976302159
-> Temp = 656011.8577487051
-> Temp = 623211.2648612697
-> Temp = 592050.7016182062
-> Temp = 562448.1665372959
-> Temp = 534325.7582104311
-> Temp = 507609.47029990953
-> Temp = 482228.99678491405
-> Temp = 458117.54694566835
-> Temp = 435211.66959838493
-> Temp = 413451.0861184657
-> Temp = 392778.5318125424
-> Temp = 373139.6052219153
-> Temp = 354482.6249608195
-> Temp = 336758.4937127785
-> Temp = 319920.5690271396
-> Temp = 303924.5405757826
-> Temp = 288728.3135469935
-> Temp = 274291.8978696438
-> Temp = 260577.30297616162
-> Temp = 247548.43782735354
-> Temp = 235171.01593598587
-> Temp = 223412.46513918656
-> Temp = 212241.84188222722
-> Temp = 201629.74978811585
-> Temp = 191548.26229871003
-> Temp = 181970.84918377452
-> Temp = 172872.3067245858
-> Temp = 164228.6913883565
-> Temp = 156017.25681893868
-> Temp = 148216.39397799174
-> Temp = 140805.57427909214
-> Temp = 133765.29556513752
-> Temp = 127077.03078688064
-> Temp = 120723.17924753661
-> Temp = 114687.02028515977
-> Temp = 108952.66927090178
-> Temp = 103505.03580735669
-> Temp = 98329.78401698885
-> Temp = 93413.2948161394
-> Temp = 88742.63007533243
-> Temp = 84305.49857156581
-> Temp = 80090.22364298752
-> Temp = 76085.71246083814
-> Temp = 72281.42683779623
-> Temp = 68667.35549590642
-> Temp = 65233.987721111094
-> Temp = 61972.288335055535
-> Temp = 58873.67391830275
-> Temp = 55929.99022238761
-> Temp = 53133.49071126823
-> Temp = 50476.816175704815
-> Temp = 47952.97536691957
-> Temp = 45555.32659857359
-> Temp = 43277.56026864491
-> Temp = 41113.68225521267
-> Temp = 39057.99814245203
-> Temp = 37105.098235329424
-> Temp = 35249.84332356295
-> Temp = 33487.3511573848
-> Temp = 31812.98359951556
Final Temp = 31812.98359951556
Placement Complete
Time Elapsed: 0:1:16.924845972
RUNNING CLEANUP
Exception in thread "main" java.lang.OutOfMemoryError: GC overhead limit exceeded
at org.cidarlab.fluigi.par.route.GridPoint.(GridPoint.java:98)
at org.cidarlab.fluigi.par.route.Grid.gridPointAt(Grid.java:70)
at org.cidarlab.fluigi.par.route.GridRouter.setObstacles(GridRouter.java:203)
at org.cidarlab.fluigi.par.route.GridRouter.route(GridRouter.java:53)
at org.cidarlab.fluigi.par.PlaceAndRoute.par(PlaceAndRoute.java:115)
at org.cidarlab.fluigi.fluigi.DesignTree.processDevice(DesignTree.java:225)
at org.cidarlab.fluigi.fluigi.DesignTree.executePAR(DesignTree.java:201)
at org.cidarlab.fluigi.fluigi.DesignTree.recursiveCreateDevice(DesignTree.java:154)
at org.cidarlab.fluigi.fluigi.DesignTree.generateDesignTree(DesignTree.java:270)
at org.cidarlab.fluigi.fluigi.Fluigi.main(Fluigi.java:254)
MINT FILE CODE-------------------------------------------------------------------------
DEVICE aj_rtpcr2
LAYER FLOW
PORT primerin_0 r=800;
PORT primerin_1 r=800;
PORT primerin_2 r=800;
PORT primerin_3 r=800;
PORT primerin_4 r=800;
PORT primerin_5 r=800;
PORT primerin_6 r=800;
PORT primerin_7 r=800;
PORT pcrmix r=800;
PORT rtmix r=800;
PORT amplifiedout_0 r=800;
PORT amplifiedout_1 r=800;
PORT amplifiedout_2 r=800;
PORT amplifiedout_3 r=800;
PORT amplifiedout_4 r=800;
PORT amplifiedout_5 r=800;
PORT amplifiedout_6 r=800;
PORT amplifiedout_7 r=800;
NODE rtpcrmix ;
NODE rtpcrprimer_0 ;
NODE rtpcrprimer_1 ;
NODE rtpcrprimer_2 ;
NODE rtpcrprimer_3 ;
NODE rtpcrprimer_4 ;
NODE rtpcrprimer_5 ;
NODE rtpcrprimer_6 ;
NODE rtpcrprimer_7 ;
NODE outstage_0 ;
NODE outstage_1 ;
NODE outstage_2 ;
NODE outstage_3 ;
NODE outstage_4 ;
NODE outstage_5 ;
NODE outstage_6 ;
NODE outstage_7 ;
NODE interaction_rtmix ;
NODE interaction_rtmix_pcrmix ;
NODE primerin_0_rtpcrmix ;
NODE primerin_1_rtpcrmix ;
NODE primerin_2_rtpcrmix ;
NODE primerin_3_rtpcrmix ;
NODE primerin_4_rtpcrmix ;
NODE primerin_5_rtpcrmix ;
NODE primerin_6_rtpcrmix ;
NODE primerin_7_rtpcrmix ;
H MIXER rtpcrmixer_0 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
H MIXER rtpcrmixer_1 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
H MIXER rtpcrmixer_2 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
H MIXER rtpcrmixer_3 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
H MIXER rtpcrmixer_4 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
H MIXER rtpcrmixer_5 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
H MIXER rtpcrmixer_6 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
H MIXER rtpcrmixer_7 bendLength=12000 bendSpacing=6000 channelWidth=200 numBends=6;
REACTION CHAMBER rtpHeatChamber_0 width=1000 length=25500;
REACTION CHAMBER rtpHeatChamber_1 width=1000 length=25500;
REACTION CHAMBER rtpHeatChamber_2 width=1000 length=25500;
REACTION CHAMBER rtpHeatChamber_3 width=1000 length=25500;
REACTION CHAMBER rtpHeatChamber_4 width=1000 length=25500;
REACTION CHAMBER rtpHeatChamber_5 width=1000 length=25500;
REACTION CHAMBER rtpHeatChamber_6 width=1000 length=25500;
REACTION CHAMBER rtpHeatChamber_7 width=1000 length=25500;
CHANNEL channel_1 from primerin_0 2 to primerin_0_rtpcrmix 1 w=100;
CHANNEL channel_2 from primerin_1 2 to primerin_1_rtpcrmix 1 w=100;
CHANNEL channel_3 from primerin_2 2 to primerin_2_rtpcrmix 1 w=100;
CHANNEL channel_4 from primerin_3 2 to primerin_3_rtpcrmix 1 w=100;
CHANNEL channel_5 from primerin_4 2 to primerin_4_rtpcrmix 1 w=100;
CHANNEL channel_6 from primerin_5 2 to primerin_5_rtpcrmix 1 w=100;
CHANNEL channel_7 from primerin_6 2 to primerin_6_rtpcrmix 1 w=100;
CHANNEL channel_8 from primerin_7 2 to primerin_7_rtpcrmix 1 w=100;
CHANNEL channel_9 from pcrmix 2 to interaction_rtmix_pcrmix 1 w=1100;
CHANNEL channel_10 from rtmix 2 to interaction_rtmix 1 w=100;
CHANNEL channel_11 from rtpcrmix 2 to primerin_0_rtpcrmix 1 w=100;
CHANNEL channel_12 from rtpcrmix 2 to primerin_1_rtpcrmix 1 w=100;
CHANNEL channel_13 from rtpcrmix 2 to primerin_2_rtpcrmix 1 w=100;
CHANNEL channel_14 from rtpcrmix 2 to primerin_3_rtpcrmix 1 w=100;
CHANNEL channel_15 from rtpcrmix 2 to primerin_4_rtpcrmix 1 w=100;
CHANNEL channel_16 from rtpcrmix 2 to primerin_5_rtpcrmix 1 w=100;
CHANNEL channel_17 from rtpcrmix 2 to primerin_6_rtpcrmix 1 w=100;
CHANNEL channel_18 from rtpcrmix 2 to primerin_7_rtpcrmix 1 w=100;
CHANNEL channel_19 from rtpcrprimer_0 2 to rtpcrmixer_0 1;
CHANNEL channel_20 from rtpcrprimer_1 2 to rtpcrmixer_1 1;
CHANNEL channel_21 from rtpcrprimer_2 2 to rtpcrmixer_2 1;
CHANNEL channel_22 from rtpcrprimer_3 2 to rtpcrmixer_3 1;
CHANNEL channel_23 from rtpcrprimer_4 2 to rtpcrmixer_4 1;
CHANNEL channel_24 from rtpcrprimer_5 2 to rtpcrmixer_5 1;
CHANNEL channel_25 from rtpcrprimer_6 2 to rtpcrmixer_6 1;
CHANNEL channel_26 from rtpcrprimer_7 2 to rtpcrmixer_7 1;
CHANNEL channel_27 from outstage_0 2 to amplifiedout_0 1;
CHANNEL channel_28 from outstage_1 2 to amplifiedout_1 1;
CHANNEL channel_29 from outstage_2 2 to amplifiedout_2 1;
CHANNEL channel_30 from outstage_3 2 to amplifiedout_3 1;
CHANNEL channel_31 from outstage_4 2 to amplifiedout_4 1;
CHANNEL channel_32 from outstage_5 2 to amplifiedout_5 1;
CHANNEL channel_33 from outstage_6 2 to amplifiedout_6 1;
CHANNEL channel_34 from outstage_7 2 to amplifiedout_7 1;
CHANNEL channel_35 from rtpcrmixer_0 2 to rtpHeatChamber_0 1;
CHANNEL channel_36 from rtpcrmixer_1 2 to rtpHeatChamber_1 1;
CHANNEL channel_37 from rtpcrmixer_2 2 to rtpHeatChamber_2 1;
CHANNEL channel_38 from rtpcrmixer_3 2 to rtpHeatChamber_3 1;
CHANNEL channel_39 from rtpcrmixer_4 2 to rtpHeatChamber_4 1;
CHANNEL channel_40 from rtpcrmixer_5 2 to rtpHeatChamber_5 1;
CHANNEL channel_41 from rtpcrmixer_6 2 to rtpHeatChamber_6 1;
CHANNEL channel_42 from rtpcrmixer_7 2 to rtpHeatChamber_7 1;
CHANNEL channel_43 from rtpHeatChamber_0 2 to outstage_0 1;
CHANNEL channel_44 from rtpHeatChamber_1 2 to outstage_1 1;
CHANNEL channel_45 from rtpHeatChamber_2 2 to outstage_2 1;
CHANNEL channel_46 from rtpHeatChamber_3 2 to outstage_3 1;
CHANNEL channel_47 from rtpHeatChamber_4 2 to outstage_4 1;
CHANNEL channel_48 from rtpHeatChamber_5 2 to outstage_5 1;
CHANNEL channel_49 from rtpHeatChamber_6 2 to outstage_6 1;
CHANNEL channel_50 from rtpHeatChamber_7 2 to outstage_7 1;
CHANNEL channel_51 from interaction_rtmix 2 to interaction_rtmix_pcrmix 1;
CHANNEL channel_52 from interaction_rtmix_pcrmix 2 to rtpcrmix 1;
CHANNEL channel_53 from primerin_0_rtpcrmix 2 to rtpcrprimer_0 1;
CHANNEL channel_54 from primerin_1_rtpcrmix 2 to rtpcrprimer_1 1;
CHANNEL channel_55 from primerin_2_rtpcrmix 2 to rtpcrprimer_2 1;
CHANNEL channel_56 from primerin_3_rtpcrmix 2 to rtpcrprimer_3 1;
CHANNEL channel_57 from primerin_4_rtpcrmix 2 to rtpcrprimer_4 1;
CHANNEL channel_58 from primerin_5_rtpcrmix 2 to rtpcrprimer_5 1;
CHANNEL channel_59 from primerin_6_rtpcrmix 2 to rtpcrprimer_6 1;
CHANNEL channel_60 from primerin_7_rtpcrmix 2 to rtpcrprimer_7 1;
END LAYER
LAYER CONTROL
PORT p3 r=800;
VALVE v1 on channel_9 w=300 l=100;
NET net1 from p3 1 to v1 4 channelWidth=100;
END LAYER
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