From 37fcb5a79c2b63fafc4fd9b8ceea1aac586887d9 Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Wed, 13 Dec 2023 11:36:31 +0100 Subject: [PATCH 01/79] Made ChrConverter more flexible (second hard-coded reference) and centralized in GlobalAppConfig. Signed-off-by: Philip R. Kensche --- Release_sophia/build-sophia.sh | 24 +- .../build-sophiaAnnotate.sh | 29 +- include/Alignment.h | 116 ++++-- include/Breakpoint.h | 19 +- include/BreakpointReduced.h | 1 + include/ChrConverter.h | 119 +++--- include/GlobalAppConfig.h | 68 ++++ include/Hg37ChrConverter.h | 44 ++ include/Hg38ChrConverter.h | 44 ++ include/MasterRefProcessor.h | 2 + include/MrefEntry.h | 2 + src/Alignment.cpp | 74 +++- src/AnnotationProcessor.cpp | 18 +- src/Breakpoint.cpp | 86 +++- src/BreakpointReduced.cpp | 20 +- src/ChrConverter.cpp | 310 ++------------ src/GlobalAppConfig.cpp | 62 +++ src/HelperFunctions.cpp | 20 +- src/Hg37ChrConverter.cpp | 377 ++++++++++++++++++ src/Hg38ChrConverter.cpp | 377 ++++++++++++++++++ src/IndexTable.cpp | 308 ++++++++++++++ src/MasterRefProcessor.cpp | 37 +- src/MrefEntry.cpp | 9 +- src/MrefEntryAnno.cpp | 1 - src/SuppAlignment.cpp | 78 +++- src/SuppAlignmentAnno.cpp | 9 +- src/SvEvent.cpp | 21 +- sophia.cpp => src/sophia.cpp | 112 ++++-- sophiaAnnotate.cpp => src/sophiaAnnotate.cpp | 108 +++-- sophiaMref.cpp => src/sophiaMref.cpp | 53 ++- 30 files changed, 1948 insertions(+), 600 deletions(-) create mode 100644 include/GlobalAppConfig.h create mode 100644 include/Hg37ChrConverter.h create mode 100644 include/Hg38ChrConverter.h create mode 100644 src/GlobalAppConfig.cpp create mode 100644 src/Hg37ChrConverter.cpp create mode 100644 src/Hg38ChrConverter.cpp create mode 100644 src/IndexTable.cpp rename sophia.cpp => src/sophia.cpp (57%) rename sophiaAnnotate.cpp => src/sophiaAnnotate.cpp (62%) rename sophiaMref.cpp => src/sophiaMref.cpp (51%) diff --git a/Release_sophia/build-sophia.sh b/Release_sophia/build-sophia.sh index 286238e..b210941 100755 --- a/Release_sophia/build-sophia.sh +++ b/Release_sophia/build-sophia.sh @@ -14,7 +14,7 @@ install_strtk CPP=x86_64-conda_cos6-linux-gnu-g++ INCLUDES="-I../include -I$CONDA_PREFIX/include" -CPP_OPTS="-L$CONDA_PREFIX/lib -std=c++1z $INCLUDES -O3 -Wall -Wextra -static -static-libgcc -static-libstdc++ -flto -c -fmessage-length=0 -Wno-attributes" +CPP_OPTS="-L$CONDA_PREFIX/lib -std=c++17 $INCLUDES -O3 -Wall -Wextra -static -static-libgcc -static-libstdc++ -flto -c -fmessage-length=0 -Wno-attributes" if [[ "${STATIC:-false}" == "true" ]]; then CPP_OPTS="-static -static-libgcc -static-libstdc++ $CPP_OPTS" @@ -23,11 +23,27 @@ fi $CPP $CPP_OPTS -o "Alignment.o" "../src/Alignment.cpp" $CPP $CPP_OPTS -o "Breakpoint.o" "../src/Breakpoint.cpp" $CPP $CPP_OPTS -o "ChosenBp.o" "../src/ChosenBp.cpp" +$CPP $CPP_OPTS -o "GlobalAppConfig.o" "../src/GlobalAppConfig.cpp" $CPP $CPP_OPTS -o "ChrConverter.o" "../src/ChrConverter.cpp" +$CPP $CPP_OPTS -o "Hg37ChrConverter.o" "../src/Hg37ChrConverter.cpp" +$CPP $CPP_OPTS -o "Hg38ChrConverter.o" "../src/Hg38ChrConverter.cpp" $CPP $CPP_OPTS -o "SamSegmentMapper.o" "../src/SamSegmentMapper.cpp" $CPP $CPP_OPTS -o "Sdust.o" "../src/Sdust.cpp" $CPP $CPP_OPTS -o "SuppAlignment.o" "../src/SuppAlignment.cpp" $CPP $CPP_OPTS -o "HelperFunctions.o" "../src/HelperFunctions.cpp" -$CPP $CPP_OPTS -o "sophia.o" "../sophia.cpp" - -$CPP -L$CONDA_PREFIX/lib -flto -o "sophia" Alignment.o Breakpoint.o ChosenBp.o ChrConverter.o SamSegmentMapper.o Sdust.o SuppAlignment.o HelperFunctions.o sophia.o -lboost_program_options +$CPP $CPP_OPTS -o "sophia.o" "../src/sophia.cpp" + +$CPP -L$CONDA_PREFIX/lib -flto -o "sophia" \ + Alignment.o \ + Breakpoint.o \ + ChosenBp.o \ + ChrConverter.o \ + Hg37ChrConverter.o \ + Hg38ChrConverter.o \ + SamSegmentMapper.o \ + Sdust.o \ + SuppAlignment.o \ + HelperFunctions.o \ + GlobalAppConfig.o \ + sophia.o \ + -lboost_program_options diff --git a/Release_sophiaAnnotate/build-sophiaAnnotate.sh b/Release_sophiaAnnotate/build-sophiaAnnotate.sh index 205fbb4..a0ccb05 100755 --- a/Release_sophiaAnnotate/build-sophiaAnnotate.sh +++ b/Release_sophiaAnnotate/build-sophiaAnnotate.sh @@ -13,7 +13,7 @@ install_strtk CPP=x86_64-conda_cos6-linux-gnu-g++ INCLUDES="-I../include -I$CONDA_PREFIX/include" -CPP_OPTS="-L$CONDA_PREFIX/lib -std=c++1z $INCLUDES -O3 -Wall -Wextra -static -static-libgcc -static-libstdc++ -flto -c -fmessage-length=0 -Wno-attributes" +CPP_OPTS="-L$CONDA_PREFIX/lib -std=c++17 $INCLUDES -O3 -Wall -Wextra -static -static-libgcc -static-libstdc++ -flto -c -fmessage-length=0 -Wno-attributes" if [[ "${STATIC:-false}" == "true" ]]; then CPP_OPTS="-static -static-libgcc -static-libstdc++ $CPP_OPTS" @@ -22,7 +22,10 @@ fi $CPP $CPP_OPTS -o "AnnotationProcessor.o" "../src/AnnotationProcessor.cpp" $CPP $CPP_OPTS -o "Breakpoint.o" "../src/Breakpoint.cpp" $CPP $CPP_OPTS -o "BreakpointReduced.o" "../src/BreakpointReduced.cpp" +$CPP $CPP_OPTS -o "GlobalAppConfig.o" "../src/GlobalAppConfig.cpp" $CPP $CPP_OPTS -o "ChrConverter.o" "../src/ChrConverter.cpp" +$CPP $CPP_OPTS -o "Hg37ChrConverter.o" "../src/Hg37ChrConverter.cpp" +$CPP $CPP_OPTS -o "Hg38ChrConverter.o" "../src/Hg38ChrConverter.cpp" $CPP $CPP_OPTS -o "DeFuzzier.o" "../src/DeFuzzier.cpp" $CPP $CPP_OPTS -o "GermlineMatch.o" "../src/GermlineMatch.cpp" $CPP $CPP_OPTS -o "MrefEntry.o" "../src/MrefEntry.cpp" @@ -32,6 +35,24 @@ $CPP $CPP_OPTS -o "SuppAlignment.o" "../src/SuppAlignment.cpp" $CPP $CPP_OPTS -o "SuppAlignmentAnno.o" "../src/SuppAlignmentAnno.cpp" $CPP $CPP_OPTS -o "SvEvent.o" "../src/SvEvent.cpp" $CPP $CPP_OPTS -o "HelperFunctions.o" "../src/HelperFunctions.cpp" -$CPP $CPP_OPTS -o "sophiaAnnotate.o" "../sophiaAnnotate.cpp" - -$CPP -L$CONDA_PREFIX/lib -flto -o "sophiaAnnotate" AnnotationProcessor.o Breakpoint.o BreakpointReduced.o ChrConverter.o DeFuzzier.o GermlineMatch.o MrefEntry.o MrefEntryAnno.o MrefMatch.o SuppAlignment.o SuppAlignmentAnno.o SvEvent.o HelperFunctions.o sophiaAnnotate.o -lz -lboost_system -lboost_iostreams +$CPP $CPP_OPTS -o "sophiaAnnotate.o" "../src/sophiaAnnotate.cpp" + +$CPP -L$CONDA_PREFIX/lib -flto -o "sophiaAnnotate" \ + AnnotationProcessor.o \ + Breakpoint.o \ + BreakpointReduced.o \ + ChrConverter.o \ + Hg37ChrConverter.o \ + Hg38ChrConverter.o \ + DeFuzzier.o \ + GermlineMatch.o \ + MrefEntry.o \ + MrefEntryAnno.o \ + MrefMatch.o \ + SuppAlignment.o \ + SuppAlignmentAnno.o \ + SvEvent.o \ + HelperFunctions.o \ + GlobalAppConfig.o \ + sophiaAnnotate.o \ + -lz -lboost_system -lboost_iostreams diff --git a/include/Alignment.h b/include/Alignment.h index efe5459..fb54456 100644 --- a/include/Alignment.h +++ b/include/Alignment.h @@ -43,133 +43,167 @@ class Alignment { public: Alignment(); + void continueConstruction(); - static int LOWQUALCLIPTHRESHOLD, BASEQUALITYTHRESHOLD, - BASEQUALITYTHRESHOLDLOW, CLIPPEDNUCLEOTIDECOUNTTHRESHOLD, + + static int + LOWQUALCLIPTHRESHOLD, + BASEQUALITYTHRESHOLD, + BASEQUALITYTHRESHOLDLOW, + CLIPPEDNUCLEOTIDECOUNTTHRESHOLD, INDELNUCLEOTIDECOUNTTHRESHOLD; + static double ISIZEMAX; + int getStartPos() const { return startPos; } + int getEndPos() const { return endPos; } + int getReadType() const { return readType; } + const vector &getReadBreakpoints() const { return readBreakpoints; } + bool isValidLine() const { return validLine; } + const string &getSamLine() const { return samLine; } + const vector &getSamChunkPositions() const { return samChunkPositions; } + bool assessOutlierMateDistance(); + int getMateChrIndex() const { return mateChrIndex; } + int getMatePos() const { return matePos; } + const vector &getReadBreakpointTypes() const { return readBreakpointTypes; } + void setChosenBp(int chosenBpLoc, int alignmentIndex); + bool isOverhangEncounteredM() const { return chosenBp->bpEncounteredM; } + int getOverhangLength() const { return chosenBp->overhangLength; } + int getOverhangStartIndex() const { return chosenBp->overhangStartIndex; } + vector generateSuppAlignments(int bpChrIndex, int bpPos); + const vector &getSupplementaryAlignments() const { return chosenBp->supplementaryAlignments; } + int getChrIndex() const { return chrIndex; } + const vector &getReadBreakpointsSizes() const { return readBreakpointSizes; } + bool isLowMapq() const { return lowMapq; } + bool isNullMapq() const { return nullMapq; } + bool isSupplementary() const { return supplementary; } + void addChildNode(int indexIn) { chosenBp->addChildNode(indexIn); } - void - addSupplementaryAlignments(const vector &suppAlignments) { + + void addSupplementaryAlignments(const vector &suppAlignments) { chosenBp->addSupplementaryAlignments(suppAlignments); } + const vector &getChildrenNodes() const { return chosenBp->childrenNodes; } + int getOriginIndex() const { return chosenBp->selfNodeIndex; } + string printOverhang() const; + double overhangComplexityMaskRatio() const; bool isInvertedMate() const { return invertedMate; } + bool isDistantMate() const { return distantMate == 1; } + private: + void mappingQualityCheck(); + bool isEventCandidate() const; + void createCigarChunks(); + void assignBreakpointsAndOverhangs(); + void qualityCheckCascade(); + bool clipCountCheck(); + bool uniqueSuppCheck(); + double overhangMedianQuality(const CigarChunk &cigarChunk) const; + template void fullMedianQuality(Iterator qualBegin, Iterator qualEnd, vector &overhangPerBaseQuality) const; + template double getMedian(Iterator begin, Iterator end) const; + void assessReadType(); + bool lowMapq; + bool nullMapq; + int distantMate; + unique_ptr chosenBp; + int chrIndex; + int readType; + int startPos, endPos; + int mateChrIndex, matePos; + string samLine; + bool validLine; + vector samChunkPositions; + string::const_iterator saCbegin, saCend; + bool hasSa; + bool supplementary; + bool fwdStrand; + bool invertedMate; + bool qualChecked; + vector cigarChunks; + vector readBreakpoints; + vector readBreakpointTypes; + vector readBreakpointSizes; + vector readBreakpointComplexityMaskRatios; + deque readBreakpointsEncounteredM; + vector readOverhangCoords; -}; -template -void -Alignment::fullMedianQuality(Iterator qualBegin, Iterator qualEnd, - vector &overhangPerBaseQuality) const { - overhangPerBaseQuality.reserve(distance(qualBegin, qualEnd)); - auto consecutiveLowQuals = 0; - for (auto cit = qualBegin; cit != qualEnd; ++cit) { - if (*cit < BASEQUALITYTHRESHOLDLOW) { // 33 + phred 11 - if (consecutiveLowQuals == 5) { - overhangPerBaseQuality.clear(); - return; - } - ++consecutiveLowQuals; - } else { - consecutiveLowQuals = 0; - } - overhangPerBaseQuality.push_back(*cit); - } -} - -// Median Code taken from http://rosettacode.org/wiki/Averages/Median#C.2B.2B -template -double -Alignment::getMedian(Iterator begin, Iterator end) const { - // this is middle for odd-length, and "upper-middle" for even length - Iterator middle = begin + (end - begin) / 2; - // This function runs in O(n) on average, according to the standard - nth_element(begin, middle, end); - if ((end - begin) % 2 != 0) { // odd length - return *middle; - } else { // even length - // the "lower middle" is the max of the lower half - Iterator lower_middle = max_element(begin, middle); - return (*middle + *lower_middle) / 2.0; - } -} +}; } /* namespace sophia */ diff --git a/include/Breakpoint.h b/include/Breakpoint.h index 3196193..5e9db4f 100644 --- a/include/Breakpoint.h +++ b/include/Breakpoint.h @@ -28,6 +28,7 @@ #include "MateInfo.h" #include "SuppAlignment.h" #include "SuppAlignmentAnno.h" +#include "ChrConverter.h" #include #include #include @@ -232,24 +233,6 @@ class Breakpoint { vector supplementsSecondary; }; -template -inline void -Breakpoint::cleanUpVector(vector &objectPool) { - // cerr << "cleaning up" << endl; - while (!objectPool.empty() && objectPool.back().isToRemove()) { - objectPool.pop_back(); - } - for (auto saIt = objectPool.begin(); saIt != objectPool.end(); ++saIt) { - if (saIt->isToRemove()) { - swap(*saIt, objectPool.back()); - } - while (!objectPool.empty() && objectPool.back().isToRemove()) { - objectPool.pop_back(); - } - } - // cerr << "done" << endl; -} - } /* namespace sophia */ #endif /* BREAKPOINT_H_ */ diff --git a/include/BreakpointReduced.h b/include/BreakpointReduced.h index 8db13c7..1312914 100644 --- a/include/BreakpointReduced.h +++ b/include/BreakpointReduced.h @@ -29,6 +29,7 @@ #include "GermlineMatch.h" #include "MrefMatch.h" #include "SuppAlignmentAnno.h" +#include "ChrConverter.h" #include #include #include diff --git a/include/ChrConverter.h b/include/ChrConverter.h index 21afddc..71be479 100644 --- a/include/ChrConverter.h +++ b/include/ChrConverter.h @@ -1,11 +1,5 @@ /* - * ChrConverter.h - * - * Created on: 28 Dec 2017 - * Author: Umut H. Toprak, DKFZ Heidelberg (Divisions of Theoretical - * Bioinformatics, Bioinformatics and Omics Data Analytics and currently - * Neuroblastoma Genomics) Copyright (C) 2018 Umut H. Toprak, Matthias - * Schlesner, Roland Eils and DKFZ Heidelberg + * Author: Philip R. Kensche, DKFZ Heidelberg (Omics IT and Data Management Core Facility) * * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as published by @@ -19,65 +13,66 @@ * * You should have received a copy of the GNU General Public License * along with this program. If not, see . - * LICENSE: GPL + * LICENSE: GPL */ -#ifndef CHRCONVERTER_H_ -#define CHRCONVERTER_H_ -#include +#ifndef _CHRCONVERTER_H_ +#define _CHRCONVERTER_H_ + +#include #include +#include -#include namespace sophia { -using namespace std; - -class ChrConverter { - public: - static inline int readChromosomeIndex(string::const_iterator startIt, - char stopChar) { - int chrIndex{0}; - if (isdigit(*startIt)) { - for (auto chr_cit = startIt; *chr_cit != stopChar; ++chr_cit) { - chrIndex = chrIndex * 10 + (*chr_cit - '0'); - } - return chrIndex; - } else { - switch (*startIt) { - case 'h': - return 999; - case 'X': - return 40; - case 'G': - for (auto cit = next(startIt, 2); *cit != '.'; ++cit) { - chrIndex = 10 * chrIndex + *cit - '0'; - } - return chrIndex; - case 'Y': - return 41; - case 'M': - ++startIt; - if (*startIt == 'T') { - return 1001; - } else { - return 1003; - } - case 'N': - return 1000; - case 'p': - return 1002; - default: - return 1003; - } - } - return 0; - } - static const array indexToChr; - static const array indexConverter; - static const array indexToChrCompressedMref; -}; - -} /* namespace sophia */ - -#endif /* CHRCONVERTER_H_ */ + using namespace std; + + /** ChrConverter contains information the names of chromosomes in an assembly. */ + class ChrConverter { + protected: + + /** The constructor should be used to initialize the fields from subclasses. It does + additional checks of the dimensions of the input vectors. */ + ChrConverter(const vector& indexToChr, + const vector& indexToChrCompressedMref, + const vector& chrSizesCompressedMref, + const vector& indexConverter); + + + public: + + virtual ~ChrConverter(); + + /** The name of the assembly. */ + static const string assembly_name; + + /** Mapping indices to chromosome names. */ + const vector indexToChr; + + /** Mapping indices to chromosome names for compressed mref files. */ + const vector indexToChrCompressedMref; + + /** Chromosome sizes in base pairs. */ + const vector chrSizesCompressedMref; + + /** Mapping chromosome names to indices. */ + const vector indexConverter; + + /** Parse chromosome index. */ + virtual int readChromosomeIndex(string::const_iterator startIt, char stopChar) const = 0; + + size_t n_chromosomes() { + return indexToChr.size(); + }; + + size_t n_chromosomes_compressed_mref() { + return indexToChrCompressedMref.size(); + }; + + + }; + +} + +#endif /* _CHRCONVERTER_H_ */ \ No newline at end of file diff --git a/include/GlobalAppConfig.h b/include/GlobalAppConfig.h new file mode 100644 index 0000000..2fa80c2 --- /dev/null +++ b/include/GlobalAppConfig.h @@ -0,0 +1,68 @@ +/* + * GlobalAppConfig.h + * + * Author: Philip R. Kensche Copyright (C) 2023 DKFZ Heidelberg + * + * This program is free software: you can redistribute it and/or modify + * it under the terms of the GNU General Public License as published by + * the Free Software Foundation, either version 3 of the License, or + * (at your option) any later version. + * + * This program is distributed in the hope that it will be useful, + * but WITHOUT ANY WARRANTY; without even the implied warranty of + * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the + * GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with this program. If not, see . + * LICENSE: GPL + */ + +#ifndef GLOBALAPPCONFIG_H +#define GLOBALAPPCONFIG_H + +#include +#include +#include "ChrConverter.h" + + +namespace sophia { + + /** Keep global application config in this singleton. This is mostly to avoid having to hand + around configurations. */ + class GlobalAppConfig { + + private: + static GlobalAppConfig *instance_; + static std::mutex mutex_; + + protected: + + GlobalAppConfig(unique_ptr chrConverter); + + ~GlobalAppConfig(); + + /** The chromosome converter. */ + const unique_ptr chrConverter; + + public: + + const ChrConverter &getChrConverter() const; + + /** Prevent copying. */ + GlobalAppConfig(GlobalAppConfig &other) = delete; + + /** Prevent assignment. */ + void operator=(const GlobalAppConfig &) = delete; + + /** Factory method. */ + static GlobalAppConfig &init(unique_ptr chrConverter); + + /** Getter. */ + static const GlobalAppConfig &getInstance(); + + }; + +} /* namespace sophia */ + +#endif /* GLOBALAPPCONFIG_H */ \ No newline at end of file diff --git a/include/Hg37ChrConverter.h b/include/Hg37ChrConverter.h new file mode 100644 index 0000000..31bfa1b --- /dev/null +++ b/include/Hg37ChrConverter.h @@ -0,0 +1,44 @@ +/* + * Author: Philip R. Kensche, DKFZ Heidelberg (Omics IT and Data Management Core Facility) + * + * This program is free software: you can redistribute it and/or modify + * it under the terms of the GNU General Public License as published by + * the Free Software Foundation, either version 3 of the License, or + * (at your option) any later version. + * + * This program is distributed in the hope that it will be useful, + * but WITHOUT ANY WARRANTY; without even the implied warranty of + * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the + * GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with this program. If not, see . + * LICENSE: GPL + */ + +#ifndef HG37CHRCONVERTER_H_ +#define HG37CHRCONVERTER_H_ + +#include "ChrConverter.h" +#include +#include + + +namespace sophia { + + using namespace std; + + class Hg37ChrConverter: public ChrConverter { + public: + + static const string assembly_name; + + Hg37ChrConverter(); + + int readChromosomeIndex(string::const_iterator startIt, char stopChar) const; + + }; + +} + +#endif /* HG37CHRCONVERTER_H_ */ \ No newline at end of file diff --git a/include/Hg38ChrConverter.h b/include/Hg38ChrConverter.h new file mode 100644 index 0000000..53db591 --- /dev/null +++ b/include/Hg38ChrConverter.h @@ -0,0 +1,44 @@ +/* + * Author: Philip R. Kensche, DKFZ Heidelberg (Omics IT and Data Management Core Facility) + * + * This program is free software: you can redistribute it and/or modify + * it under the terms of the GNU General Public License as published by + * the Free Software Foundation, either version 3 of the License, or + * (at your option) any later version. + * + * This program is distributed in the hope that it will be useful, + * but WITHOUT ANY WARRANTY; without even the implied warranty of + * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the + * GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with this program. If not, see . + * LICENSE: GPL + */ + +#ifndef HG38CHRCONVERTER_H_ +#define HG38CHRCONVERTER_H_ + +#include "ChrConverter.h" +#include +#include + + +namespace sophia { + + using namespace std; + + class Hg38ChrConverter: public ChrConverter { + public: + + static const string assembly_name; + + Hg38ChrConverter(); + + int readChromosomeIndex(string::const_iterator startIt, char stopChar) const; + + }; + +} + +#endif /* HG38CHRCONVERTER_H_ */ \ No newline at end of file diff --git a/include/MasterRefProcessor.h b/include/MasterRefProcessor.h index deb370d..2601905 100644 --- a/include/MasterRefProcessor.h +++ b/include/MasterRefProcessor.h @@ -26,6 +26,7 @@ #define MASTERREFPROCESSOR_H_ #include "SuppAlignment.h" +#include "ChrConverter.h" #include #include #include @@ -47,6 +48,7 @@ class MasterRefProcessor { MasterRefProcessor(const vector &filesIn, const string &outputRootName, const string &version, const int defaultReadLengthIn); + ~MasterRefProcessor() = default; private: diff --git a/include/MrefEntry.h b/include/MrefEntry.h index 0d32a2d..03d7d4f 100644 --- a/include/MrefEntry.h +++ b/include/MrefEntry.h @@ -27,6 +27,7 @@ #include "BreakpointReduced.h" #include "SuppAlignment.h" +#include "ChrConverter.h" #include #include @@ -39,6 +40,7 @@ class MrefEntry { static int NUMPIDS; static int DEFAULTREADLENGTH; static boost::format doubleFormatter; + MrefEntry(); void addEntry(Breakpoint &tmpBreakpoint, int fileIndex); void addEntry(BreakpointReduced &tmpBreakpoint, int fileIndex); diff --git a/src/Alignment.cpp b/src/Alignment.cpp index a9e049a..0bd32fc 100644 --- a/src/Alignment.cpp +++ b/src/Alignment.cpp @@ -24,6 +24,7 @@ #include "Alignment.h" #include "ChrConverter.h" +#include "GlobalAppConfig.h" #include "HelperFunctions.h" #include "MateInfo.h" #include "Sdust.h" @@ -35,19 +36,34 @@ namespace sophia { using namespace std; -int Alignment::LOWQUALCLIPTHRESHOLD{}, Alignment::BASEQUALITYTHRESHOLD{}, - Alignment::BASEQUALITYTHRESHOLDLOW{}, // +int Alignment::LOWQUALCLIPTHRESHOLD{}, + Alignment::BASEQUALITYTHRESHOLD{}, + Alignment::BASEQUALITYTHRESHOLDLOW{}, Alignment::CLIPPEDNUCLEOTIDECOUNTTHRESHOLD{}, Alignment::INDELNUCLEOTIDECOUNTTHRESHOLD{}; double Alignment::ISIZEMAX{}; Alignment::Alignment() - : lowMapq{false}, nullMapq{true}, distantMate{0}, chosenBp{nullptr}, - chrIndex{0}, readType{0}, startPos{0}, endPos{0}, mateChrIndex{0}, - matePos{0}, samLine{}, validLine{error_terminating_getline(cin, samLine)}, - samChunkPositions{}, saCbegin{}, saCend{}, hasSa{false}, - supplementary{false}, fwdStrand{true}, invertedMate{false}, qualChecked{ - false} { + : lowMapq(false), + nullMapq(true), + distantMate(0), + chosenBp(nullptr), + chrIndex(0), + readType(0), + startPos(0), + endPos(0), + mateChrIndex(0), + matePos(0), + samLine(), + validLine(error_terminating_getline(cin, samLine)), + samChunkPositions(), + saCbegin(), + saCend(), + hasSa(false), + supplementary(false), + fwdStrand(true), + invertedMate(false), + qualChecked(false) { if (validLine) { auto index = 0; for (auto it = samLine.cbegin(); it != samLine.cend(); ++it) { @@ -56,7 +72,7 @@ Alignment::Alignment() } ++index; } - chrIndex = ChrConverter::readChromosomeIndex( + chrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex( next(samLine.cbegin(), samChunkPositions[1] + 1), '\t'); } } @@ -157,7 +173,7 @@ Alignment::continueConstruction() { if (samLine[1 + samChunkPositions[5]] == '=') { mateChrIndex = chrIndex; } else { - mateChrIndex = ChrConverter::readChromosomeIndex( + mateChrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex( next(samLine.cbegin(), 1 + samChunkPositions[5]), '\t'); } } @@ -709,4 +725,42 @@ Alignment::overhangComplexityMaskRatio() const { return maskedIntervalsTotal / fullSizesTotal; } + +template +void +Alignment::fullMedianQuality(Iterator qualBegin, Iterator qualEnd, + vector &overhangPerBaseQuality) const { + overhangPerBaseQuality.reserve(distance(qualBegin, qualEnd)); + auto consecutiveLowQuals = 0; + for (auto cit = qualBegin; cit != qualEnd; ++cit) { + if (*cit < BASEQUALITYTHRESHOLDLOW) { // 33 + phred 11 + if (consecutiveLowQuals == 5) { + overhangPerBaseQuality.clear(); + return; + } + ++consecutiveLowQuals; + } else { + consecutiveLowQuals = 0; + } + overhangPerBaseQuality.push_back(*cit); + } +} + +// Median Code taken from http://rosettacode.org/wiki/Averages/Median#C.2B.2B +template +double +Alignment::getMedian(Iterator begin, Iterator end) const { + // this is middle for odd-length, and "upper-middle" for even length + Iterator middle = begin + (end - begin) / 2; + // This function runs in O(n) on average, according to the standard + nth_element(begin, middle, end); + if ((end - begin) % 2 != 0) { // odd length + return *middle; + } else { // even length + // the "lower middle" is the max of the lower half + Iterator lower_middle = max_element(begin, middle); + return (*middle + *lower_middle) / 2.0; + } +} + } /* namespace sophia */ diff --git a/src/AnnotationProcessor.cpp b/src/AnnotationProcessor.cpp index 7f5a9b1..d67f78b 100644 --- a/src/AnnotationProcessor.cpp +++ b/src/AnnotationProcessor.cpp @@ -25,6 +25,7 @@ #include "Breakpoint.h" #include "HelperFunctions.h" #include "SuppAlignment.h" +#include "GlobalAppConfig.h" #include #include #include @@ -57,12 +58,13 @@ AnnotationProcessor::AnnotationProcessor(const string &tumorResultsIn, tumorGzHandle->push(*tumorInputHandle); string line; auto lineIndex = 0; + const ChrConverter& chrConverter = GlobalAppConfig::getInstance().getChrConverter(); while (error_terminating_getline(*tumorGzHandle, line)) { if (line.front() == '#') { continue; }; Breakpoint tmpBp{line, true}; - auto chrIndex = ChrConverter::indexConverter[tmpBp.getChrIndex()]; + auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; if (chrIndex < 0) { continue; } @@ -121,6 +123,7 @@ AnnotationProcessor::AnnotationProcessor( controlGzHandle->push(*controlInputHandle); string line; auto lineIndex = 0; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); while (error_terminating_getline(*controlGzHandle, line)) { if (line.front() == '#') { continue; @@ -217,7 +220,7 @@ AnnotationProcessor::AnnotationProcessor( } } } - auto chrIndex = ChrConverter::indexConverter[tmpBp.getChrIndex()]; + auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; controlResults[chrIndex].push_back(tmpBp); ++lineIndex; } @@ -237,7 +240,7 @@ AnnotationProcessor::AnnotationProcessor( continue; }; Breakpoint tmpBp{line, true}; - auto chrIndex = ChrConverter::indexConverter[tmpBp.getChrIndex()]; + auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; if (chrIndex < 0) { continue; } @@ -310,7 +313,8 @@ AnnotationProcessor::searchSa(int chrIndex, int dbIndex, } return; } - auto saChrIndex = ChrConverter::indexConverter[sa.getChrIndex()]; + auto saChrIndex = GlobalAppConfig::getInstance().getChrConverter(). + indexConverter[sa.getChrIndex()]; if (saChrIndex < 0) { return; } @@ -544,7 +548,8 @@ AnnotationProcessor::searchMrefHitsNew(const BreakpointReduced &bpIn, int distanceThreshold, int conservativeDistanceThreshold, vector> &mref) { - auto convertedChrIndex = ChrConverter::indexConverter[bpIn.getChrIndex()]; + auto convertedChrIndex = GlobalAppConfig::getInstance().getChrConverter(). + indexConverter[bpIn.getChrIndex()]; vector suppMatches{}; if (convertedChrIndex < 0) { return MrefMatch{0, 0, 10000, suppMatches}; @@ -680,7 +685,8 @@ AnnotationProcessor::searchGermlineHitsNew(const BreakpointReduced &bpIn, if (NOCONTROLMODE) { return dummyMatchTrue; } - auto convertedChrIndex = ChrConverter::indexConverter[bpIn.getChrIndex()]; + auto convertedChrIndex = GlobalAppConfig::getInstance().getChrConverter(). + indexConverter[bpIn.getChrIndex()]; if (convertedChrIndex < 0) { return dummyMatchFalse; } diff --git a/src/Breakpoint.cpp b/src/Breakpoint.cpp index 1cfccc5..ab0e985 100644 --- a/src/Breakpoint.cpp +++ b/src/Breakpoint.cpp @@ -24,6 +24,7 @@ #include "Breakpoint.h" #include "ChrConverter.h" +#include "GlobalAppConfig.h" #include "strtk.hpp" #include #include @@ -56,15 +57,52 @@ bool Breakpoint::PROPERPAIRCOMPENSATIONMODE{false}; int Breakpoint::bpindex{0}; Breakpoint::Breakpoint(int chrIndexIn, int posIn) - : covFinalized{false}, missingInfoBp{false}, chrIndex{chrIndexIn}, - pos{posIn}, normalSpans{0}, lowQualSpansSoft{0}, lowQualSpansHard{0}, - unpairedBreaksSoft{0}, unpairedBreaksHard{0}, breaksShortIndel{0}, - lowQualBreaksSoft{0}, lowQualBreaksHard{0}, repetitiveOverhangBreaks{0}, - pairedBreaksSoft{0}, pairedBreaksHard{0}, leftSideDiscordantCandidates{0}, - rightSideDiscordantCandidates{0}, mateSupport{0}, leftCoverage{0}, - rightCoverage{0}, totalLowMapqHardClips{0}, - hitsInMref{-1}, germline{false}, poolLeft{}, poolRight{}, - poolLowQualLeft{}, poolLowQualRight{} {} + : covFinalized{false}, + missingInfoBp{false}, + chrIndex{chrIndexIn}, + pos{posIn}, + normalSpans{0}, + lowQualSpansSoft{0}, + lowQualSpansHard{0}, + unpairedBreaksSoft{0}, + unpairedBreaksHard{0}, + breaksShortIndel{0}, + lowQualBreaksSoft{0}, + lowQualBreaksHard{0}, + repetitiveOverhangBreaks{0}, + pairedBreaksSoft{0}, + pairedBreaksHard{0}, + leftSideDiscordantCandidates{0}, + rightSideDiscordantCandidates{0}, + mateSupport{0}, + leftCoverage{0}, + rightCoverage{0}, + totalLowMapqHardClips{0}, + hitsInMref{-1}, + germline{false}, + poolLeft{}, + poolRight{}, + poolLowQualLeft{}, + poolLowQualRight{} {} + + +template +inline void +Breakpoint::cleanUpVector(vector &objectPool) { + // cerr << "cleaning up" << endl; + while (!objectPool.empty() && objectPool.back().isToRemove()) { + objectPool.pop_back(); + } + for (auto saIt = objectPool.begin(); saIt != objectPool.end(); ++saIt) { + if (saIt->isToRemove()) { + swap(*saIt, objectPool.back()); + } + while (!objectPool.empty() && objectPool.back().isToRemove()) { + objectPool.pop_back(); + } + } + // cerr << "done" << endl; +} void Breakpoint::addSoftAlignment(shared_ptr alignmentIn) { @@ -163,7 +201,7 @@ void Breakpoint::printBreakpointReport(const string &overhangStr) { string res{}; res.reserve(350); - res.append(ChrConverter::indexToChr[chrIndex]).append("\t"); + res.append(GlobalAppConfig::getInstance().getChrConverter().indexToChr[chrIndex]).append("\t"); res.append(strtk::type_to_string(pos)).append("\t"); res.append(strtk::type_to_string(pos + 1)).append("\t"); @@ -1104,12 +1142,26 @@ Breakpoint::collectMateSupportHelper( } Breakpoint::Breakpoint(const string &bpIn, bool ignoreOverhang) - : covFinalized{true}, missingInfoBp{false}, chrIndex{0}, pos{0}, - normalSpans{0}, lowQualSpansSoft{0}, lowQualSpansHard{0}, - unpairedBreaksSoft{0}, unpairedBreaksHard{0}, breaksShortIndel{0}, - lowQualBreaksSoft{0}, lowQualBreaksHard{0}, repetitiveOverhangBreaks{0}, - pairedBreaksSoft{0}, pairedBreaksHard{0}, mateSupport{0}, leftCoverage{0}, - rightCoverage{0}, hitsInMref{0}, germline{false} { + : covFinalized{true}, + missingInfoBp{false}, + chrIndex{0}, + pos{0}, + normalSpans{0}, + lowQualSpansSoft{0}, + lowQualSpansHard{0}, + unpairedBreaksSoft{0}, + unpairedBreaksHard{0}, + breaksShortIndel{0}, + lowQualBreaksSoft{0}, + lowQualBreaksHard{0}, + repetitiveOverhangBreaks{0}, + pairedBreaksSoft{0}, + pairedBreaksHard{0}, + mateSupport{0}, + leftCoverage{0}, + rightCoverage{0}, + hitsInMref{0}, + germline{false} { auto index = 0; vector bpChunkPositions{}; bpChunkPositions.reserve(7); @@ -1119,7 +1171,7 @@ Breakpoint::Breakpoint(const string &bpIn, bool ignoreOverhang) } ++index; } - chrIndex = ChrConverter::readChromosomeIndex(bpIn.cbegin(), '\t'); + chrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex(bpIn.cbegin(), '\t'); for (auto i = bpChunkPositions[0] + 1; i < bpChunkPositions[1]; ++i) { pos = pos * 10 + (bpIn[i] - '0'); diff --git a/src/BreakpointReduced.cpp b/src/BreakpointReduced.cpp index a0dc308..1a887ab 100644 --- a/src/BreakpointReduced.cpp +++ b/src/BreakpointReduced.cpp @@ -24,6 +24,7 @@ #include "Breakpoint.h" #include "ChrConverter.h" +#include "GlobalAppConfig.h" #include "strtk.hpp" #include #include @@ -57,17 +58,9 @@ sophia::BreakpointReduced::BreakpointReduced(const Breakpoint &tmpBp, pairedBreaksHard{tmpBp.getPairedBreaksHard()}, mateSupport{tmpBp.getMateSupport()}, leftCoverage{tmpBp.getLeftCoverage()}, - rightCoverage{tmpBp.getRightCoverage()}, mrefHits{MrefMatch{ - -1, - -1, - 10000, - {}, - }}, - germlineInfo{GermlineMatch{ - 0.0, - 0.0, - {}, - }}, + rightCoverage{tmpBp.getRightCoverage()}, + mrefHits{MrefMatch{-1, -1, 10000, {}, }}, + germlineInfo{GermlineMatch{0.0, 0.0, {}, }}, suppAlignments{} { for (const auto &sa : tmpBp.getDoubleSidedMatches()) { if (sa.getChrIndex() < 1002) { @@ -175,10 +168,11 @@ BreakpointReduced::testOverhangBasedCandidacy() const { } string -BreakpointReduced::printOverhang(double germlineClonality, int numHits, +BreakpointReduced::printOverhang(double germlineClonality, + int numHits, const string &overhang) const { string res{"##"}; - res.append(ChrConverter::indexToChr[chrIndex]).append("\t"); + res.append(GlobalAppConfig::getInstance().getChrConverter().indexToChr[chrIndex]).append("\t"); res.append(strtk::type_to_string(pos - 1)).append("\t"); res.append(strtk::type_to_string(pos)).append("\t"); if (germlineClonality > 0.1) { diff --git a/src/ChrConverter.cpp b/src/ChrConverter.cpp index a42e50b..c20c238 100644 --- a/src/ChrConverter.cpp +++ b/src/ChrConverter.cpp @@ -1,11 +1,5 @@ /* - * ChrConverter.cpp - * - * Created on: 28 Dec 2017 - * Author: Umut H. Toprak, DKFZ Heidelberg (Divisions of Theoretical - * Bioinformatics, Bioinformatics and Omics Data Analytics and currently - * Neuroblastoma Genomics) Copyright (C) 2018 Umut H. Toprak, Matthias - * Schlesner, Roland Eils and DKFZ Heidelberg + * Author: Philip R. Kensche, DKFZ Heidelberg (Omics IT and Data Management Core Facility) * * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as published by @@ -19,290 +13,34 @@ * * You should have received a copy of the GNU General Public License * along with this program. If not, see . - * LICENSE: GPL + * LICENSE: GPL */ -#include +#include "ChrConverter.h" +#include +#include +#include namespace sophia { -using namespace std; - -const array ChrConverter::indexToChr{ - "0", "1", "2", "3", "4", - "5", "6", "7", "8", "9", - "10", "11", "12", "13", "14", - "15", "16", "17", "18", "19", - "20", "21", "22", "23", "24", - "25", "26", "27", "28", "29", - "30", "31", "32", "33", "34", - "35", "36", "37", "38", "39", - "X", "Y", "42", "43", "44", - "45", "46", "47", "48", "49", - "50", "51", "52", "53", "54", - "55", "56", "57", "58", "59", - "60", "61", "62", "63", "64", - "65", "66", "67", "68", "69", - "70", "71", "72", "73", "74", - "75", "76", "77", "78", "79", - "80", "81", "82", "83", "84", - "85", "86", "87", "88", "89", - "90", "91", "92", "93", "94", - "95", "96", "97", "98", "99", - "100", "101", "102", "103", "104", - "105", "106", "107", "108", "109", - "110", "111", "112", "113", "114", - "115", "116", "117", "118", "119", - "120", "121", "122", "123", "124", - "125", "126", "127", "128", "129", - "130", "131", "132", "133", "134", - "135", "136", "137", "138", "139", - "140", "141", "142", "143", "144", - "145", "146", "147", "148", "149", - "150", "151", "152", "153", "154", - "155", "156", "157", "158", "159", - "160", "161", "162", "163", "164", - "165", "166", "167", "168", "169", - "170", "171", "172", "173", "174", - "175", "176", "177", "178", "179", - "180", "181", "182", "183", "184", - "185", "186", "187", "188", "189", - "190", "GL000191.1", "GL000192.1", "GL000193.1", "GL000194.1", - "GL000195.1", "GL000196.1", "GL000197.1", "GL000198.1", "GL000199.1", - "GL000200.1", "GL000201.1", "GL000202.1", "GL000203.1", "GL000204.1", - "GL000205.1", "GL000206.1", "GL000207.1", "GL000208.1", "GL000209.1", - "GL000210.1", "GL000211.1", "GL000212.1", "GL000213.1", "GL000214.1", - "GL000215.1", "GL000216.1", "GL000217.1", "GL000218.1", "GL000219.1", - "GL000220.1", "GL000221.1", "GL000222.1", "GL000223.1", "GL000224.1", - "GL000225.1", "GL000226.1", "GL000227.1", "GL000228.1", "GL000229.1", - "GL000230.1", "GL000231.1", "GL000232.1", "GL000233.1", "GL000234.1", - "GL000235.1", "GL000236.1", "GL000237.1", "GL000238.1", "GL000239.1", - "GL000240.1", "GL000241.1", "GL000242.1", "GL000243.1", "GL000244.1", - "GL000245.1", "GL000246.1", "GL000247.1", "GL000248.1", "GL000249.1", - "250", "251", "252", "253", "254", - "255", "256", "257", "258", "259", - "260", "261", "262", "263", "264", - "265", "266", "267", "268", "269", - "270", "271", "272", "273", "274", - "275", "276", "277", "278", "279", - "280", "281", "282", "283", "284", - "285", "286", "287", "288", "289", - "290", "291", "292", "293", "294", - "295", "296", "297", "298", "299", - "300", "301", "302", "303", "304", - "305", "306", "307", "308", "309", - "310", "311", "312", "313", "314", - "315", "316", "317", "318", "319", - "320", "321", "322", "323", "324", - "325", "326", "327", "328", "329", - "330", "331", "332", "333", "334", - "335", "336", "337", "338", "339", - "340", "341", "342", "343", "344", - "345", "346", "347", "348", "349", - "350", "351", "352", "353", "354", - "355", "356", "357", "358", "359", - "360", "361", "362", "363", "364", - "365", "366", "367", "368", "369", - "370", "371", "372", "373", "374", - "375", "376", "377", "378", "379", - "380", "381", "382", "383", "384", - "385", "386", "387", "388", "389", - "390", "391", "392", "393", "394", - "395", "396", "397", "398", "399", - "400", "401", "402", "403", "404", - "405", "406", "407", "408", "409", - "410", "411", "412", "413", "414", - "415", "416", "417", "418", "419", - "420", "421", "422", "423", "424", - "425", "426", "427", "428", "429", - "430", "431", "432", "433", "434", - "435", "436", "437", "438", "439", - "440", "441", "442", "443", "444", - "445", "446", "447", "448", "449", - "450", "451", "452", "453", "454", - "455", "456", "457", "458", "459", - "460", "461", "462", "463", "464", - "465", "466", "467", "468", "469", - "470", "471", "472", "473", "474", - "475", "476", "477", "478", "479", - "480", "481", "482", "483", "484", - "485", "486", "487", "488", "489", - "490", "491", "492", "493", "494", - "495", "496", "497", "498", "499", - "500", "501", "502", "503", "504", - "505", "506", "507", "508", "509", - "510", "511", "512", "513", "514", - "515", "516", "517", "518", "519", - "520", "521", "522", "523", "524", - "525", "526", "527", "528", "529", - "530", "531", "532", "533", "534", - "535", "536", "537", "538", "539", - "540", "541", "542", "543", "544", - "545", "546", "547", "548", "549", - "550", "551", "552", "553", "554", - "555", "556", "557", "558", "559", - "560", "561", "562", "563", "564", - "565", "566", "567", "568", "569", - "570", "571", "572", "573", "574", - "575", "576", "577", "578", "579", - "580", "581", "582", "583", "584", - "585", "586", "587", "588", "589", - "590", "591", "592", "593", "594", - "595", "596", "597", "598", "599", - "600", "601", "602", "603", "604", - "605", "606", "607", "608", "609", - "610", "611", "612", "613", "614", - "615", "616", "617", "618", "619", - "620", "621", "622", "623", "624", - "625", "626", "627", "628", "629", - "630", "631", "632", "633", "634", - "635", "636", "637", "638", "639", - "640", "641", "642", "643", "644", - "645", "646", "647", "648", "649", - "650", "651", "652", "653", "654", - "655", "656", "657", "658", "659", - "660", "661", "662", "663", "664", - "665", "666", "667", "668", "669", - "670", "671", "672", "673", "674", - "675", "676", "677", "678", "679", - "680", "681", "682", "683", "684", - "685", "686", "687", "688", "689", - "690", "691", "692", "693", "694", - "695", "696", "697", "698", "699", - "700", "701", "702", "703", "704", - "705", "706", "707", "708", "709", - "710", "711", "712", "713", "714", - "715", "716", "717", "718", "719", - "720", "721", "722", "723", "724", - "725", "726", "727", "728", "729", - "730", "731", "732", "733", "734", - "735", "736", "737", "738", "739", - "740", "741", "742", "743", "744", - "745", "746", "747", "748", "749", - "750", "751", "752", "753", "754", - "755", "756", "757", "758", "759", - "760", "761", "762", "763", "764", - "765", "766", "767", "768", "769", - "770", "771", "772", "773", "774", - "775", "776", "777", "778", "779", - "780", "781", "782", "783", "784", - "785", "786", "787", "788", "789", - "790", "791", "792", "793", "794", - "795", "796", "797", "798", "799", - "800", "801", "802", "803", "804", - "805", "806", "807", "808", "809", - "810", "811", "812", "813", "814", - "815", "816", "817", "818", "819", - "820", "821", "822", "823", "824", - "825", "826", "827", "828", "829", - "830", "831", "832", "833", "834", - "835", "836", "837", "838", "839", - "840", "841", "842", "843", "844", - "845", "846", "847", "848", "849", - "850", "851", "852", "853", "854", - "855", "856", "857", "858", "859", - "860", "861", "862", "863", "864", - "865", "866", "867", "868", "869", - "870", "871", "872", "873", "874", - "875", "876", "877", "878", "879", - "880", "881", "882", "883", "884", - "885", "886", "887", "888", "889", - "890", "891", "892", "893", "894", - "895", "896", "897", "898", "899", - "900", "901", "902", "903", "904", - "905", "906", "907", "908", "909", - "910", "911", "912", "913", "914", - "915", "916", "917", "918", "919", - "920", "921", "922", "923", "924", - "925", "926", "927", "928", "929", - "930", "931", "932", "933", "934", - "935", "936", "937", "938", "939", - "940", "941", "942", "943", "944", - "945", "946", "947", "948", "949", - "950", "951", "952", "953", "954", - "955", "956", "957", "958", "959", - "960", "961", "962", "963", "964", - "965", "966", "967", "968", "969", - "970", "971", "972", "973", "974", - "975", "976", "977", "978", "979", - "980", "981", "982", "983", "984", - "985", "986", "987", "988", "989", - "990", "991", "992", "993", "994", - "995", "996", "997", "998", "hs37d5", - "NC_007605", "MT", "phiX174", "INVALID"}; + using namespace std; -const array ChrConverter::indexToChrCompressedMref{ - "1", "2", "3", "4", "5", - "6", "7", "8", "9", "10", - "11", "12", "13", "14", "15", - "16", "17", "18", "19", "20", - "21", "22", "X", "Y", "GL000191.1", - "GL000192.1", "GL000193.1", "GL000194.1", "GL000195.1", "GL000196.1", - "GL000197.1", "GL000198.1", "GL000199.1", "GL000200.1", "GL000201.1", - "GL000202.1", "GL000203.1", "GL000204.1", "GL000205.1", "GL000206.1", - "GL000207.1", "GL000208.1", "GL000209.1", "GL000210.1", "GL000211.1", - "GL000212.1", "GL000213.1", "GL000214.1", "GL000215.1", "GL000216.1", - "GL000217.1", "GL000218.1", "GL000219.1", "GL000220.1", "GL000221.1", - "GL000222.1", "GL000223.1", "GL000224.1", "GL000225.1", "GL000226.1", - "GL000227.1", "GL000228.1", "GL000229.1", "GL000230.1", "GL000231.1", - "GL000232.1", "GL000233.1", "GL000234.1", "GL000235.1", "GL000236.1", - "GL000237.1", "GL000238.1", "GL000239.1", "GL000240.1", "GL000241.1", - "GL000242.1", "GL000243.1", "GL000244.1", "GL000245.1", "GL000246.1", - "GL000247.1", "GL000248.1", "GL000249.1", "hs37d5", "NC_007605"}; + ChrConverter::ChrConverter(const vector &indexToChr, + const vector &indexToChrCompressedMref, + const vector &chrSizesCompressedMref, + const vector &indexConverter) : + indexToChr(indexToChr), + indexToChrCompressedMref(indexToChrCompressedMref), + chrSizesCompressedMref(chrSizesCompressedMref), + indexConverter(indexConverter) { + if (indexToChr.size() != indexConverter.size()) + throw invalid_argument( + "indexToChr and indexConverter must have the same size"); + if (indexToChrCompressedMref.size() != chrSizesCompressedMref.size()) + throw invalid_argument( + "indexToChrCompressedMref and chrSizesCompressedMref must have the same size"); + } -const array ChrConverter::indexConverter{ - -2, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, - 18, 19, 20, 21, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, 22, 23, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, - 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, - 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, - 80, 81, 82, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, 83, 84, -2, -2, -2}; + ChrConverter::~ChrConverter() {} -} /* namespace sophia */ +} \ No newline at end of file diff --git a/src/GlobalAppConfig.cpp b/src/GlobalAppConfig.cpp new file mode 100644 index 0000000..ed19901 --- /dev/null +++ b/src/GlobalAppConfig.cpp @@ -0,0 +1,62 @@ +/* + * GlobalAppConfig.cpp + * + * Author: Philip R. Kensche Copyright (C) 2023 DKFZ Heidelberg + * + * This program is free software: you can redistribute it and/or modify + * it under the terms of the GNU General Public License as published by + * the Free Software Foundation, either version 3 of the License, or + * (at your option) any later version. + * + * This program is distributed in the hope that it will be useful, + * but WITHOUT ANY WARRANTY; without even the implied warranty of + * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the + * GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with this program. If not, see . + * LICENSE: GPL + */ + +#include "GlobalAppConfig.h" +#include +#include +#include + + +namespace sophia { + + using namespace std; + + GlobalAppConfig* GlobalAppConfig::instance_ = nullptr; + std::mutex GlobalAppConfig::mutex_ = std::mutex(); + + const ChrConverter &GlobalAppConfig::getChrConverter() const { + return *chrConverter; + } + + GlobalAppConfig::GlobalAppConfig(unique_ptr chrConverter): + chrConverter(move(chrConverter)) {} + + GlobalAppConfig::~GlobalAppConfig() {} + + GlobalAppConfig &GlobalAppConfig::init(unique_ptr chrConverter) + { + lock_guard lock(mutex_); + if (GlobalAppConfig::instance_ == nullptr) { + GlobalAppConfig::instance_ = new GlobalAppConfig(move(chrConverter)); + } else { + throw new logic_error("GlobalAppConfig already initialized"); + } + return *GlobalAppConfig::instance_; + } + + const GlobalAppConfig &GlobalAppConfig::getInstance() { + if (GlobalAppConfig::instance_ == nullptr) { + throw new logic_error("GlobalAppConfig not initialized"); + } else { + return *GlobalAppConfig::instance_; + } + } + +} \ No newline at end of file diff --git a/src/HelperFunctions.cpp b/src/HelperFunctions.cpp index 260a144..f5bdd57 100644 --- a/src/HelperFunctions.cpp +++ b/src/HelperFunctions.cpp @@ -24,18 +24,18 @@ namespace sophia { -using namespace std; + using namespace std; -istream & -error_terminating_getline(istream &is, string &str) { - getline(is, str); + istream & + error_terminating_getline(istream &is, string &str) { + getline(is, str); - if (is.bad()) { - perror("Error reading line from file"); - exit(EXITCODE_IOERROR); - } + if (is.bad()) { + perror("Error reading line from file"); + exit(EXITCODE_IOERROR); + } - return is; -} + return is; + } } /* namespace sophia */ diff --git a/src/Hg37ChrConverter.cpp b/src/Hg37ChrConverter.cpp new file mode 100644 index 0000000..e57dddd --- /dev/null +++ b/src/Hg37ChrConverter.cpp @@ -0,0 +1,377 @@ +/* + * Author: Philip R. Kensche, DKFZ Heidelberg (Omics IT and Data Management Core Facility) + * + * This program is free software: you can redistribute it and/or modify + * it under the terms of the GNU General Public License as published by + * the Free Software Foundation, either version 3 of the License, or + * (at your option) any later version. + * + * This program is distributed in the hope that it will be useful, + * but WITHOUT ANY WARRANTY; without even the implied warranty of + * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the + * GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with this program. If not, see . + * LICENSE: GPL + */ + +#include "Hg37ChrConverter.h" + +#include +#include + + +namespace sophia { + + using namespace std; + + namespace hg37 { + + static const vector indexToChr { + "0", "1", "2", "3", "4", + "5", "6", "7", "8", "9", + "10", "11", "12", "13", "14", + "15", "16", "17", "18", "19", + "20", "21", "22", "23", "24", + "25", "26", "27", "28", "29", + "30", "31", "32", "33", "34", + "35", "36", "37", "38", "39", + "X", "Y", "42", "43", "44", + "45", "46", "47", "48", "49", + "50", "51", "52", "53", "54", + "55", "56", "57", "58", "59", + "60", "61", "62", "63", "64", + "65", "66", "67", "68", "69", + "70", "71", "72", "73", "74", + "75", "76", "77", "78", "79", + "80", "81", "82", "83", "84", + "85", "86", "87", "88", "89", + "90", "91", "92", "93", "94", + "95", "96", "97", "98", "99", + "100", "101", "102", "103", "104", + "105", "106", "107", "108", "109", + "110", "111", "112", "113", "114", + "115", "116", "117", "118", "119", + "120", "121", "122", "123", "124", + "125", "126", "127", "128", "129", + "130", "131", "132", "133", "134", + "135", "136", "137", "138", "139", + "140", "141", "142", "143", "144", + "145", "146", "147", "148", "149", + "150", "151", "152", "153", "154", + "155", "156", "157", "158", "159", + "160", "161", "162", "163", "164", + "165", "166", "167", "168", "169", + "170", "171", "172", "173", "174", + "175", "176", "177", "178", "179", + "180", "181", "182", "183", "184", + "185", "186", "187", "188", "189", + "190", "GL000191.1", "GL000192.1", "GL000193.1", "GL000194.1", + "GL000195.1", "GL000196.1", "GL000197.1", "GL000198.1", "GL000199.1", + "GL000200.1", "GL000201.1", "GL000202.1", "GL000203.1", "GL000204.1", + "GL000205.1", "GL000206.1", "GL000207.1", "GL000208.1", "GL000209.1", + "GL000210.1", "GL000211.1", "GL000212.1", "GL000213.1", "GL000214.1", + "GL000215.1", "GL000216.1", "GL000217.1", "GL000218.1", "GL000219.1", + "GL000220.1", "GL000221.1", "GL000222.1", "GL000223.1", "GL000224.1", + "GL000225.1", "GL000226.1", "GL000227.1", "GL000228.1", "GL000229.1", + "GL000230.1", "GL000231.1", "GL000232.1", "GL000233.1", "GL000234.1", + "GL000235.1", "GL000236.1", "GL000237.1", "GL000238.1", "GL000239.1", + "GL000240.1", "GL000241.1", "GL000242.1", "GL000243.1", "GL000244.1", + "GL000245.1", "GL000246.1", "GL000247.1", "GL000248.1", "GL000249.1", + "250", "251", "252", "253", "254", + "255", "256", "257", "258", "259", + "260", "261", "262", "263", "264", + "265", "266", "267", "268", "269", + "270", "271", "272", "273", "274", + "275", "276", "277", "278", "279", + "280", "281", "282", "283", "284", + "285", "286", "287", "288", "289", + "290", "291", "292", "293", "294", + "295", "296", "297", "298", "299", + "300", "301", "302", "303", "304", + "305", "306", "307", "308", "309", + "310", "311", "312", "313", "314", + "315", "316", "317", "318", "319", + "320", "321", "322", "323", "324", + "325", "326", "327", "328", "329", + "330", "331", "332", "333", "334", + "335", "336", "337", "338", "339", + "340", "341", "342", "343", "344", + "345", "346", "347", "348", "349", + "350", "351", "352", "353", "354", + "355", "356", "357", "358", "359", + "360", "361", "362", "363", "364", + "365", "366", "367", "368", "369", + "370", "371", "372", "373", "374", + "375", "376", "377", "378", "379", + "380", "381", "382", "383", "384", + "385", "386", "387", "388", "389", + "390", "391", "392", "393", "394", + "395", "396", "397", "398", "399", + "400", "401", "402", "403", "404", + "405", "406", "407", "408", "409", + "410", "411", "412", "413", "414", + "415", "416", "417", "418", "419", + "420", "421", "422", "423", "424", + "425", "426", "427", "428", "429", + "430", "431", "432", "433", "434", + "435", "436", "437", "438", "439", + "440", "441", "442", "443", "444", + "445", "446", "447", "448", "449", + "450", "451", "452", "453", "454", + "455", "456", "457", "458", "459", + "460", "461", "462", "463", "464", + "465", "466", "467", "468", "469", + "470", "471", "472", "473", "474", + "475", "476", "477", "478", "479", + "480", "481", "482", "483", "484", + "485", "486", "487", "488", "489", + "490", "491", "492", "493", "494", + "495", "496", "497", "498", "499", + "500", "501", "502", "503", "504", + "505", "506", "507", "508", "509", + "510", "511", "512", "513", "514", + "515", "516", "517", "518", "519", + "520", "521", "522", "523", "524", + "525", "526", "527", "528", "529", + "530", "531", "532", "533", "534", + "535", "536", "537", "538", "539", + "540", "541", "542", "543", "544", + "545", "546", "547", "548", "549", + "550", "551", "552", "553", "554", + "555", "556", "557", "558", "559", + "560", "561", "562", "563", "564", + "565", "566", "567", "568", "569", + "570", "571", "572", "573", "574", + "575", "576", "577", "578", "579", + "580", "581", "582", "583", "584", + "585", "586", "587", "588", "589", + "590", "591", "592", "593", "594", + "595", "596", "597", "598", "599", + "600", "601", "602", "603", "604", + "605", "606", "607", "608", "609", + "610", "611", "612", "613", "614", + "615", "616", "617", "618", "619", + "620", "621", "622", "623", "624", + "625", "626", "627", "628", "629", + "630", "631", "632", "633", "634", + "635", "636", "637", "638", "639", + "640", "641", "642", "643", "644", + "645", "646", "647", "648", "649", + "650", "651", "652", "653", "654", + "655", "656", "657", "658", "659", + "660", "661", "662", "663", "664", + "665", "666", "667", "668", "669", + "670", "671", "672", "673", "674", + "675", "676", "677", "678", "679", + "680", "681", "682", "683", "684", + "685", "686", "687", "688", "689", + "690", "691", "692", "693", "694", + "695", "696", "697", "698", "699", + "700", "701", "702", "703", "704", + "705", "706", "707", "708", "709", + "710", "711", "712", "713", "714", + "715", "716", "717", "718", "719", + "720", "721", "722", "723", "724", + "725", "726", "727", "728", "729", + "730", "731", "732", "733", "734", + "735", "736", "737", "738", "739", + "740", "741", "742", "743", "744", + "745", "746", "747", "748", "749", + "750", "751", "752", "753", "754", + "755", "756", "757", "758", "759", + "760", "761", "762", "763", "764", + "765", "766", "767", "768", "769", + "770", "771", "772", "773", "774", + "775", "776", "777", "778", "779", + "780", "781", "782", "783", "784", + "785", "786", "787", "788", "789", + "790", "791", "792", "793", "794", + "795", "796", "797", "798", "799", + "800", "801", "802", "803", "804", + "805", "806", "807", "808", "809", + "810", "811", "812", "813", "814", + "815", "816", "817", "818", "819", + "820", "821", "822", "823", "824", + "825", "826", "827", "828", "829", + "830", "831", "832", "833", "834", + "835", "836", "837", "838", "839", + "840", "841", "842", "843", "844", + "845", "846", "847", "848", "849", + "850", "851", "852", "853", "854", + "855", "856", "857", "858", "859", + "860", "861", "862", "863", "864", + "865", "866", "867", "868", "869", + "870", "871", "872", "873", "874", + "875", "876", "877", "878", "879", + "880", "881", "882", "883", "884", + "885", "886", "887", "888", "889", + "890", "891", "892", "893", "894", + "895", "896", "897", "898", "899", + "900", "901", "902", "903", "904", + "905", "906", "907", "908", "909", + "910", "911", "912", "913", "914", + "915", "916", "917", "918", "919", + "920", "921", "922", "923", "924", + "925", "926", "927", "928", "929", + "930", "931", "932", "933", "934", + "935", "936", "937", "938", "939", + "940", "941", "942", "943", "944", + "945", "946", "947", "948", "949", + "950", "951", "952", "953", "954", + "955", "956", "957", "958", "959", + "960", "961", "962", "963", "964", + "965", "966", "967", "968", "969", + "970", "971", "972", "973", "974", + "975", "976", "977", "978", "979", + "980", "981", "982", "983", "984", + "985", "986", "987", "988", "989", + "990", "991", "992", "993", "994", + "995", "996", "997", "998", "hs37d5", + "NC_007605", "MT", "phiX174", "INVALID"}; + + static const vector indexToChrCompressedMref { + "1", "2", "3", "4", "5", + "6", "7", "8", "9", "10", + "11", "12", "13", "14", "15", + "16", "17", "18", "19", "20", + "21", "22", "X", "Y", "GL000191.1", + "GL000192.1", "GL000193.1", "GL000194.1", "GL000195.1", "GL000196.1", + "GL000197.1", "GL000198.1", "GL000199.1", "GL000200.1", "GL000201.1", + "GL000202.1", "GL000203.1", "GL000204.1", "GL000205.1", "GL000206.1", + "GL000207.1", "GL000208.1", "GL000209.1", "GL000210.1", "GL000211.1", + "GL000212.1", "GL000213.1", "GL000214.1", "GL000215.1", "GL000216.1", + "GL000217.1", "GL000218.1", "GL000219.1", "GL000220.1", "GL000221.1", + "GL000222.1", "GL000223.1", "GL000224.1", "GL000225.1", "GL000226.1", + "GL000227.1", "GL000228.1", "GL000229.1", "GL000230.1", "GL000231.1", + "GL000232.1", "GL000233.1", "GL000234.1", "GL000235.1", "GL000236.1", + "GL000237.1", "GL000238.1", "GL000239.1", "GL000240.1", "GL000241.1", + "GL000242.1", "GL000243.1", "GL000244.1", "GL000245.1", "GL000246.1", + "GL000247.1", "GL000248.1", "GL000249.1", "hs37d5", "NC_007605"}; + + static const vector chrSizesCompressedMref { + 249250622, 243199374, 198022431, 191154277, 180915261, 171115068, + 159138664, 146364023, 141213432, 135534748, 135006517, 133851896, + 115169879, 107349541, 102531393, 90354754, 81195211, 78077249, + 59128984, 63025521, 48129896, 51304567, 155270561, 59373567, + 106434, 547497, 189790, 191470, 182897, 38915, + 37176, 90086, 169875, 187036, 36149, 40104, + 37499, 81311, 174589, 41002, 4263, 92690, + 159170, 27683, 166567, 186859, 164240, 137719, + 172546, 172295, 172150, 161148, 179199, 161803, + 155398, 186862, 180456, 179694, 211174, 15009, + 128375, 129121, 19914, 43692, 27387, 40653, + 45942, 40532, 34475, 41935, 45868, 39940, + 33825, 41934, 42153, 43524, 43342, 39930, + 36652, 38155, 36423, 39787, 38503, 35477944, + 171824}; + + static const vector indexConverter { + -2, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, + 18, 19, 20, 21, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, 22, 23, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, + 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, + 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, + 80, 81, 82, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, 83, 84, -2, -2, -2}; + + } /* namespace hg37 */ + + const string Hg37ChrConverter::assembly_name = "hg37"; + + Hg37ChrConverter::Hg37ChrConverter() + : ChrConverter(hg37::indexToChr, + hg37::indexToChrCompressedMref, + hg37::chrSizesCompressedMref, + hg37::indexConverter) {} + + /* This is parsing code. It takes a position in a character stream, and translates the + following character(s) into index positions (see ChrConverter::indexToChr). */ + int Hg37ChrConverter::readChromosomeIndex(string::const_iterator startIt, + char stopChar) const { + int chrIndex {0}; + if (isdigit(*startIt)) { + for (auto chr_cit = startIt; *chr_cit != stopChar; ++chr_cit) { + chrIndex = chrIndex * 10 + (*chr_cit - '0'); + } + return chrIndex; + } else { + switch (*startIt) { + case 'h': + return 999; + case 'X': + return 40; + case 'G': + for (auto cit = next(startIt, 2); *cit != '.'; ++cit) { + chrIndex = 10 * chrIndex + *cit - '0'; + } + return chrIndex; + case 'Y': + return 41; + case 'M': + ++startIt; + if (*startIt == 'T') { + return 1001; + } else { + return 1003; + } + case 'N': + return 1000; + case 'p': + return 1002; + default: + return 1003; + } + } + return 0; + } + + +} /* namespace sophia */ diff --git a/src/Hg38ChrConverter.cpp b/src/Hg38ChrConverter.cpp new file mode 100644 index 0000000..2ca7ade --- /dev/null +++ b/src/Hg38ChrConverter.cpp @@ -0,0 +1,377 @@ +/* + * Author: Philip R. Kensche, DKFZ Heidelberg (Omics IT and Data Management Core Facility) + * + * This program is free software: you can redistribute it and/or modify + * it under the terms of the GNU General Public License as published by + * the Free Software Foundation, either version 3 of the License, or + * (at your option) any later version. + * + * This program is distributed in the hope that it will be useful, + * but WITHOUT ANY WARRANTY; without even the implied warranty of + * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the + * GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with this program. If not, see . + * LICENSE: GPL + */ + +#include "Hg38ChrConverter.h" + +#include +#include + + +namespace sophia { + + using namespace std; + + namespace hg38 { + + static const vector indexToChr { + "0", "1", "2", "3", "4", + "5", "6", "7", "8", "9", + "10", "11", "12", "13", "14", + "15", "16", "17", "18", "19", + "20", "21", "22", "23", "24", + "25", "26", "27", "28", "29", + "30", "31", "32", "33", "34", + "35", "36", "37", "38", "39", + "X", "Y", "42", "43", "44", + "45", "46", "47", "48", "49", + "50", "51", "52", "53", "54", + "55", "56", "57", "58", "59", + "60", "61", "62", "63", "64", + "65", "66", "67", "68", "69", + "70", "71", "72", "73", "74", + "75", "76", "77", "78", "79", + "80", "81", "82", "83", "84", + "85", "86", "87", "88", "89", + "90", "91", "92", "93", "94", + "95", "96", "97", "98", "99", + "100", "101", "102", "103", "104", + "105", "106", "107", "108", "109", + "110", "111", "112", "113", "114", + "115", "116", "117", "118", "119", + "120", "121", "122", "123", "124", + "125", "126", "127", "128", "129", + "130", "131", "132", "133", "134", + "135", "136", "137", "138", "139", + "140", "141", "142", "143", "144", + "145", "146", "147", "148", "149", + "150", "151", "152", "153", "154", + "155", "156", "157", "158", "159", + "160", "161", "162", "163", "164", + "165", "166", "167", "168", "169", + "170", "171", "172", "173", "174", + "175", "176", "177", "178", "179", + "180", "181", "182", "183", "184", + "185", "186", "187", "188", "189", + "190", "GL000191.1", "GL000192.1", "GL000193.1", "GL000194.1", + "GL000195.1", "GL000196.1", "GL000197.1", "GL000198.1", "GL000199.1", + "GL000200.1", "GL000201.1", "GL000202.1", "GL000203.1", "GL000204.1", + "GL000205.1", "GL000206.1", "GL000207.1", "GL000208.1", "GL000209.1", + "GL000210.1", "GL000211.1", "GL000212.1", "GL000213.1", "GL000214.1", + "GL000215.1", "GL000216.1", "GL000217.1", "GL000218.1", "GL000219.1", + "GL000220.1", "GL000221.1", "GL000222.1", "GL000223.1", "GL000224.1", + "GL000225.1", "GL000226.1", "GL000227.1", "GL000228.1", "GL000229.1", + "GL000230.1", "GL000231.1", "GL000232.1", "GL000233.1", "GL000234.1", + "GL000235.1", "GL000236.1", "GL000237.1", "GL000238.1", "GL000239.1", + "GL000240.1", "GL000241.1", "GL000242.1", "GL000243.1", "GL000244.1", + "GL000245.1", "GL000246.1", "GL000247.1", "GL000248.1", "GL000249.1", + "250", "251", "252", "253", "254", + "255", "256", "257", "258", "259", + "260", "261", "262", "263", "264", + "265", "266", "267", "268", "269", + "270", "271", "272", "273", "274", + "275", "276", "277", "278", "279", + "280", "281", "282", "283", "284", + "285", "286", "287", "288", "289", + "290", "291", "292", "293", "294", + "295", "296", "297", "298", "299", + "300", "301", "302", "303", "304", + "305", "306", "307", "308", "309", + "310", "311", "312", "313", "314", + "315", "316", "317", "318", "319", + "320", "321", "322", "323", "324", + "325", "326", "327", "328", "329", + "330", "331", "332", "333", "334", + "335", "336", "337", "338", "339", + "340", "341", "342", "343", "344", + "345", "346", "347", "348", "349", + "350", "351", "352", "353", "354", + "355", "356", "357", "358", "359", + "360", "361", "362", "363", "364", + "365", "366", "367", "368", "369", + "370", "371", "372", "373", "374", + "375", "376", "377", "378", "379", + "380", "381", "382", "383", "384", + "385", "386", "387", "388", "389", + "390", "391", "392", "393", "394", + "395", "396", "397", "398", "399", + "400", "401", "402", "403", "404", + "405", "406", "407", "408", "409", + "410", "411", "412", "413", "414", + "415", "416", "417", "418", "419", + "420", "421", "422", "423", "424", + "425", "426", "427", "428", "429", + "430", "431", "432", "433", "434", + "435", "436", "437", "438", "439", + "440", "441", "442", "443", "444", + "445", "446", "447", "448", "449", + "450", "451", "452", "453", "454", + "455", "456", "457", "458", "459", + "460", "461", "462", "463", "464", + "465", "466", "467", "468", "469", + "470", "471", "472", "473", "474", + "475", "476", "477", "478", "479", + "480", "481", "482", "483", "484", + "485", "486", "487", "488", "489", + "490", "491", "492", "493", "494", + "495", "496", "497", "498", "499", + "500", "501", "502", "503", "504", + "505", "506", "507", "508", "509", + "510", "511", "512", "513", "514", + "515", "516", "517", "518", "519", + "520", "521", "522", "523", "524", + "525", "526", "527", "528", "529", + "530", "531", "532", "533", "534", + "535", "536", "537", "538", "539", + "540", "541", "542", "543", "544", + "545", "546", "547", "548", "549", + "550", "551", "552", "553", "554", + "555", "556", "557", "558", "559", + "560", "561", "562", "563", "564", + "565", "566", "567", "568", "569", + "570", "571", "572", "573", "574", + "575", "576", "577", "578", "579", + "580", "581", "582", "583", "584", + "585", "586", "587", "588", "589", + "590", "591", "592", "593", "594", + "595", "596", "597", "598", "599", + "600", "601", "602", "603", "604", + "605", "606", "607", "608", "609", + "610", "611", "612", "613", "614", + "615", "616", "617", "618", "619", + "620", "621", "622", "623", "624", + "625", "626", "627", "628", "629", + "630", "631", "632", "633", "634", + "635", "636", "637", "638", "639", + "640", "641", "642", "643", "644", + "645", "646", "647", "648", "649", + "650", "651", "652", "653", "654", + "655", "656", "657", "658", "659", + "660", "661", "662", "663", "664", + "665", "666", "667", "668", "669", + "670", "671", "672", "673", "674", + "675", "676", "677", "678", "679", + "680", "681", "682", "683", "684", + "685", "686", "687", "688", "689", + "690", "691", "692", "693", "694", + "695", "696", "697", "698", "699", + "700", "701", "702", "703", "704", + "705", "706", "707", "708", "709", + "710", "711", "712", "713", "714", + "715", "716", "717", "718", "719", + "720", "721", "722", "723", "724", + "725", "726", "727", "728", "729", + "730", "731", "732", "733", "734", + "735", "736", "737", "738", "739", + "740", "741", "742", "743", "744", + "745", "746", "747", "748", "749", + "750", "751", "752", "753", "754", + "755", "756", "757", "758", "759", + "760", "761", "762", "763", "764", + "765", "766", "767", "768", "769", + "770", "771", "772", "773", "774", + "775", "776", "777", "778", "779", + "780", "781", "782", "783", "784", + "785", "786", "787", "788", "789", + "790", "791", "792", "793", "794", + "795", "796", "797", "798", "799", + "800", "801", "802", "803", "804", + "805", "806", "807", "808", "809", + "810", "811", "812", "813", "814", + "815", "816", "817", "818", "819", + "820", "821", "822", "823", "824", + "825", "826", "827", "828", "829", + "830", "831", "832", "833", "834", + "835", "836", "837", "838", "839", + "840", "841", "842", "843", "844", + "845", "846", "847", "848", "849", + "850", "851", "852", "853", "854", + "855", "856", "857", "858", "859", + "860", "861", "862", "863", "864", + "865", "866", "867", "868", "869", + "870", "871", "872", "873", "874", + "875", "876", "877", "878", "879", + "880", "881", "882", "883", "884", + "885", "886", "887", "888", "889", + "890", "891", "892", "893", "894", + "895", "896", "897", "898", "899", + "900", "901", "902", "903", "904", + "905", "906", "907", "908", "909", + "910", "911", "912", "913", "914", + "915", "916", "917", "918", "919", + "920", "921", "922", "923", "924", + "925", "926", "927", "928", "929", + "930", "931", "932", "933", "934", + "935", "936", "937", "938", "939", + "940", "941", "942", "943", "944", + "945", "946", "947", "948", "949", + "950", "951", "952", "953", "954", + "955", "956", "957", "958", "959", + "960", "961", "962", "963", "964", + "965", "966", "967", "968", "969", + "970", "971", "972", "973", "974", + "975", "976", "977", "978", "979", + "980", "981", "982", "983", "984", + "985", "986", "987", "988", "989", + "990", "991", "992", "993", "994", + "995", "996", "997", "998", "hs37d5", + "NC_007605", "MT", "phiX174", "INVALID"}; + + static const vector indexToChrCompressedMref { + "1", "2", "3", "4", "5", + "6", "7", "8", "9", "10", + "11", "12", "13", "14", "15", + "16", "17", "18", "19", "20", + "21", "22", "X", "Y", "GL000191.1", + "GL000192.1", "GL000193.1", "GL000194.1", "GL000195.1", "GL000196.1", + "GL000197.1", "GL000198.1", "GL000199.1", "GL000200.1", "GL000201.1", + "GL000202.1", "GL000203.1", "GL000204.1", "GL000205.1", "GL000206.1", + "GL000207.1", "GL000208.1", "GL000209.1", "GL000210.1", "GL000211.1", + "GL000212.1", "GL000213.1", "GL000214.1", "GL000215.1", "GL000216.1", + "GL000217.1", "GL000218.1", "GL000219.1", "GL000220.1", "GL000221.1", + "GL000222.1", "GL000223.1", "GL000224.1", "GL000225.1", "GL000226.1", + "GL000227.1", "GL000228.1", "GL000229.1", "GL000230.1", "GL000231.1", + "GL000232.1", "GL000233.1", "GL000234.1", "GL000235.1", "GL000236.1", + "GL000237.1", "GL000238.1", "GL000239.1", "GL000240.1", "GL000241.1", + "GL000242.1", "GL000243.1", "GL000244.1", "GL000245.1", "GL000246.1", + "GL000247.1", "GL000248.1", "GL000249.1", "hs37d5", "NC_007605"}; + + static const vector chrSizesCompressedMref { + 249250622, 243199374, 198022431, 191154277, 180915261, 171115068, + 159138664, 146364023, 141213432, 135534748, 135006517, 133851896, + 115169879, 107349541, 102531393, 90354754, 81195211, 78077249, + 59128984, 63025521, 48129896, 51304567, 155270561, 59373567, + 106434, 547497, 189790, 191470, 182897, 38915, + 37176, 90086, 169875, 187036, 36149, 40104, + 37499, 81311, 174589, 41002, 4263, 92690, + 159170, 27683, 166567, 186859, 164240, 137719, + 172546, 172295, 172150, 161148, 179199, 161803, + 155398, 186862, 180456, 179694, 211174, 15009, + 128375, 129121, 19914, 43692, 27387, 40653, + 45942, 40532, 34475, 41935, 45868, 39940, + 33825, 41934, 42153, 43524, 43342, 39930, + 36652, 38155, 36423, 39787, 38503, 35477944, + 171824}; + + static const vector indexConverter { + -2, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, + 18, 19, 20, 21, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, 22, 23, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, + 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, + 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, + 80, 81, 82, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, 83, 84, -2, -2, -2}; + + } /* namespace hg38 */ + + const string Hg38ChrConverter::assembly_name = "hg38"; + + Hg38ChrConverter::Hg38ChrConverter() + : ChrConverter(hg38::indexToChr, + hg38::indexToChrCompressedMref, + hg38::chrSizesCompressedMref, + hg38::indexConverter) {} + + /* This is parsing code. It takes a position in a character stream, and translates the + following character(s) into index positions (see ChrConverter::indexToChr). */ + int Hg38ChrConverter::readChromosomeIndex(string::const_iterator startIt, + char stopChar) const { + int chrIndex {0}; + if (isdigit(*startIt)) { + for (auto chr_cit = startIt; *chr_cit != stopChar; ++chr_cit) { + chrIndex = chrIndex * 10 + (*chr_cit - '0'); + } + return chrIndex; + } else { + switch (*startIt) { + case 'h': + return 999; + case 'X': + return 40; + case 'G': + for (auto cit = next(startIt, 2); *cit != '.'; ++cit) { + chrIndex = 10 * chrIndex + *cit - '0'; + } + return chrIndex; + case 'Y': + return 41; + case 'M': + ++startIt; + if (*startIt == 'T') { + return 1001; + } else { + return 1003; + } + case 'N': + return 1000; + case 'p': + return 1002; + default: + return 1003; + } + } + return 0; + } + + +} /* namespace sophia */ diff --git a/src/IndexTable.cpp b/src/IndexTable.cpp new file mode 100644 index 0000000..50a7f06 --- /dev/null +++ b/src/IndexTable.cpp @@ -0,0 +1,308 @@ +/* + * Hg37IndexTable.cpp + * + * Created on: 28 Dec 2017 + * Author: Umut H. Toprak, DKFZ Heidelberg (Divisions of Theoretical + * Bioinformatics, Bioinformatics and Omics Data Analytics and currently + * Neuroblastoma Genomics) Copyright (C) 2018 Umut H. Toprak, Matthias + * Schlesner, Roland Eils and DKFZ Heidelberg + * + * This program is free software: you can redistribute it and/or modify + * it under the terms of the GNU General Public License as published by + * the Free Software Foundation, either version 3 of the License, or + * (at your option) any later version. + * + * This program is distributed in the hope that it will be useful, + * but WITHOUT ANY WARRANTY; without even the implied warranty of + * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the + * GNU General Public License for more details. + * + * You should have received a copy of the GNU General Public License + * along with this program. If not, see . + * LICENSE: GPL + */ + +#include + +namespace sophia { + + using namespace std; + + const array Hg37IndexTable::indexToChr { + "0", "1", "2", "3", "4", + "5", "6", "7", "8", "9", + "10", "11", "12", "13", "14", + "15", "16", "17", "18", "19", + "20", "21", "22", "23", "24", + "25", "26", "27", "28", "29", + "30", "31", "32", "33", "34", + "35", "36", "37", "38", "39", + "X", "Y", "42", "43", "44", + "45", "46", "47", "48", "49", + "50", "51", "52", "53", "54", + "55", "56", "57", "58", "59", + "60", "61", "62", "63", "64", + "65", "66", "67", "68", "69", + "70", "71", "72", "73", "74", + "75", "76", "77", "78", "79", + "80", "81", "82", "83", "84", + "85", "86", "87", "88", "89", + "90", "91", "92", "93", "94", + "95", "96", "97", "98", "99", + "100", "101", "102", "103", "104", + "105", "106", "107", "108", "109", + "110", "111", "112", "113", "114", + "115", "116", "117", "118", "119", + "120", "121", "122", "123", "124", + "125", "126", "127", "128", "129", + "130", "131", "132", "133", "134", + "135", "136", "137", "138", "139", + "140", "141", "142", "143", "144", + "145", "146", "147", "148", "149", + "150", "151", "152", "153", "154", + "155", "156", "157", "158", "159", + "160", "161", "162", "163", "164", + "165", "166", "167", "168", "169", + "170", "171", "172", "173", "174", + "175", "176", "177", "178", "179", + "180", "181", "182", "183", "184", + "185", "186", "187", "188", "189", + "190", "GL000191.1", "GL000192.1", "GL000193.1", "GL000194.1", + "GL000195.1", "GL000196.1", "GL000197.1", "GL000198.1", "GL000199.1", + "GL000200.1", "GL000201.1", "GL000202.1", "GL000203.1", "GL000204.1", + "GL000205.1", "GL000206.1", "GL000207.1", "GL000208.1", "GL000209.1", + "GL000210.1", "GL000211.1", "GL000212.1", "GL000213.1", "GL000214.1", + "GL000215.1", "GL000216.1", "GL000217.1", "GL000218.1", "GL000219.1", + "GL000220.1", "GL000221.1", "GL000222.1", "GL000223.1", "GL000224.1", + "GL000225.1", "GL000226.1", "GL000227.1", "GL000228.1", "GL000229.1", + "GL000230.1", "GL000231.1", "GL000232.1", "GL000233.1", "GL000234.1", + "GL000235.1", "GL000236.1", "GL000237.1", "GL000238.1", "GL000239.1", + "GL000240.1", "GL000241.1", "GL000242.1", "GL000243.1", "GL000244.1", + "GL000245.1", "GL000246.1", "GL000247.1", "GL000248.1", "GL000249.1", + "250", "251", "252", "253", "254", + "255", "256", "257", "258", "259", + "260", "261", "262", "263", "264", + "265", "266", "267", "268", "269", + "270", "271", "272", "273", "274", + "275", "276", "277", "278", "279", + "280", "281", "282", "283", "284", + "285", "286", "287", "288", "289", + "290", "291", "292", "293", "294", + "295", "296", "297", "298", "299", + "300", "301", "302", "303", "304", + "305", "306", "307", "308", "309", + "310", "311", "312", "313", "314", + "315", "316", "317", "318", "319", + "320", "321", "322", "323", "324", + "325", "326", "327", "328", "329", + "330", "331", "332", "333", "334", + "335", "336", "337", "338", "339", + "340", "341", "342", "343", "344", + "345", "346", "347", "348", "349", + "350", "351", "352", "353", "354", + "355", "356", "357", "358", "359", + "360", "361", "362", "363", "364", + "365", "366", "367", "368", "369", + "370", "371", "372", "373", "374", + "375", "376", "377", "378", "379", + "380", "381", "382", "383", "384", + "385", "386", "387", "388", "389", + "390", "391", "392", "393", "394", + "395", "396", "397", "398", "399", + "400", "401", "402", "403", "404", + "405", "406", "407", "408", "409", + "410", "411", "412", "413", "414", + "415", "416", "417", "418", "419", + "420", "421", "422", "423", "424", + "425", "426", "427", "428", "429", + "430", "431", "432", "433", "434", + "435", "436", "437", "438", "439", + "440", "441", "442", "443", "444", + "445", "446", "447", "448", "449", + "450", "451", "452", "453", "454", + "455", "456", "457", "458", "459", + "460", "461", "462", "463", "464", + "465", "466", "467", "468", "469", + "470", "471", "472", "473", "474", + "475", "476", "477", "478", "479", + "480", "481", "482", "483", "484", + "485", "486", "487", "488", "489", + "490", "491", "492", "493", "494", + "495", "496", "497", "498", "499", + "500", "501", "502", "503", "504", + "505", "506", "507", "508", "509", + "510", "511", "512", "513", "514", + "515", "516", "517", "518", "519", + "520", "521", "522", "523", "524", + "525", "526", "527", "528", "529", + "530", "531", "532", "533", "534", + "535", "536", "537", "538", "539", + "540", "541", "542", "543", "544", + "545", "546", "547", "548", "549", + "550", "551", "552", "553", "554", + "555", "556", "557", "558", "559", + "560", "561", "562", "563", "564", + "565", "566", "567", "568", "569", + "570", "571", "572", "573", "574", + "575", "576", "577", "578", "579", + "580", "581", "582", "583", "584", + "585", "586", "587", "588", "589", + "590", "591", "592", "593", "594", + "595", "596", "597", "598", "599", + "600", "601", "602", "603", "604", + "605", "606", "607", "608", "609", + "610", "611", "612", "613", "614", + "615", "616", "617", "618", "619", + "620", "621", "622", "623", "624", + "625", "626", "627", "628", "629", + "630", "631", "632", "633", "634", + "635", "636", "637", "638", "639", + "640", "641", "642", "643", "644", + "645", "646", "647", "648", "649", + "650", "651", "652", "653", "654", + "655", "656", "657", "658", "659", + "660", "661", "662", "663", "664", + "665", "666", "667", "668", "669", + "670", "671", "672", "673", "674", + "675", "676", "677", "678", "679", + "680", "681", "682", "683", "684", + "685", "686", "687", "688", "689", + "690", "691", "692", "693", "694", + "695", "696", "697", "698", "699", + "700", "701", "702", "703", "704", + "705", "706", "707", "708", "709", + "710", "711", "712", "713", "714", + "715", "716", "717", "718", "719", + "720", "721", "722", "723", "724", + "725", "726", "727", "728", "729", + "730", "731", "732", "733", "734", + "735", "736", "737", "738", "739", + "740", "741", "742", "743", "744", + "745", "746", "747", "748", "749", + "750", "751", "752", "753", "754", + "755", "756", "757", "758", "759", + "760", "761", "762", "763", "764", + "765", "766", "767", "768", "769", + "770", "771", "772", "773", "774", + "775", "776", "777", "778", "779", + "780", "781", "782", "783", "784", + "785", "786", "787", "788", "789", + "790", "791", "792", "793", "794", + "795", "796", "797", "798", "799", + "800", "801", "802", "803", "804", + "805", "806", "807", "808", "809", + "810", "811", "812", "813", "814", + "815", "816", "817", "818", "819", + "820", "821", "822", "823", "824", + "825", "826", "827", "828", "829", + "830", "831", "832", "833", "834", + "835", "836", "837", "838", "839", + "840", "841", "842", "843", "844", + "845", "846", "847", "848", "849", + "850", "851", "852", "853", "854", + "855", "856", "857", "858", "859", + "860", "861", "862", "863", "864", + "865", "866", "867", "868", "869", + "870", "871", "872", "873", "874", + "875", "876", "877", "878", "879", + "880", "881", "882", "883", "884", + "885", "886", "887", "888", "889", + "890", "891", "892", "893", "894", + "895", "896", "897", "898", "899", + "900", "901", "902", "903", "904", + "905", "906", "907", "908", "909", + "910", "911", "912", "913", "914", + "915", "916", "917", "918", "919", + "920", "921", "922", "923", "924", + "925", "926", "927", "928", "929", + "930", "931", "932", "933", "934", + "935", "936", "937", "938", "939", + "940", "941", "942", "943", "944", + "945", "946", "947", "948", "949", + "950", "951", "952", "953", "954", + "955", "956", "957", "958", "959", + "960", "961", "962", "963", "964", + "965", "966", "967", "968", "969", + "970", "971", "972", "973", "974", + "975", "976", "977", "978", "979", + "980", "981", "982", "983", "984", + "985", "986", "987", "988", "989", + "990", "991", "992", "993", "994", + "995", "996", "997", "998", "hs37d5", + "NC_007605", "MT", "phiX174", "INVALID"}; + + const array Hg37IndexTable::indexToChrCompressedMref { + "1", "2", "3", "4", "5", + "6", "7", "8", "9", "10", + "11", "12", "13", "14", "15", + "16", "17", "18", "19", "20", + "21", "22", "X", "Y", "GL000191.1", + "GL000192.1", "GL000193.1", "GL000194.1", "GL000195.1", "GL000196.1", + "GL000197.1", "GL000198.1", "GL000199.1", "GL000200.1", "GL000201.1", + "GL000202.1", "GL000203.1", "GL000204.1", "GL000205.1", "GL000206.1", + "GL000207.1", "GL000208.1", "GL000209.1", "GL000210.1", "GL000211.1", + "GL000212.1", "GL000213.1", "GL000214.1", "GL000215.1", "GL000216.1", + "GL000217.1", "GL000218.1", "GL000219.1", "GL000220.1", "GL000221.1", + "GL000222.1", "GL000223.1", "GL000224.1", "GL000225.1", "GL000226.1", + "GL000227.1", "GL000228.1", "GL000229.1", "GL000230.1", "GL000231.1", + "GL000232.1", "GL000233.1", "GL000234.1", "GL000235.1", "GL000236.1", + "GL000237.1", "GL000238.1", "GL000239.1", "GL000240.1", "GL000241.1", + "GL000242.1", "GL000243.1", "GL000244.1", "GL000245.1", "GL000246.1", + "GL000247.1", "GL000248.1", "GL000249.1", "hs37d5", "NC_007605"}; + + const array Hg37IndexTable::indexConverter { + -2, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, + 18, 19, 20, 21, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, 22, 23, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, + 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, + 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, + 80, 81, 82, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, + -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, 83, 84, -2, -2, -2}; + +} /* namespace sophia */ diff --git a/src/MasterRefProcessor.cpp b/src/MasterRefProcessor.cpp index 37d27c6..b4ac069 100644 --- a/src/MasterRefProcessor.cpp +++ b/src/MasterRefProcessor.cpp @@ -23,6 +23,7 @@ */ #include "ChrConverter.h" +#include "GlobalAppConfig.h" #include "DeFuzzier.h" #include "HelperFunctions.h" #include "strtk.hpp" @@ -42,26 +43,14 @@ MasterRefProcessor::MasterRefProcessor(const vector &filesIn, const string &version, const int defaultReadLengthIn) : NUMPIDS{static_cast(filesIn.size())}, - DEFAULTREADLENGTH{defaultReadLengthIn}, mrefDb{} { - const vector CHRSIZES{ - 249250622, 243199374, 198022431, 191154277, 180915261, 171115068, - 159138664, 146364023, 141213432, 135534748, 135006517, 133851896, - 115169879, 107349541, 102531393, 90354754, 81195211, 78077249, - 59128984, 63025521, 48129896, 51304567, 155270561, 59373567, - 106434, 547497, 189790, 191470, 182897, 38915, - 37176, 90086, 169875, 187036, 36149, 40104, - 37499, 81311, 174589, 41002, 4263, 92690, - 159170, 27683, 166567, 186859, 164240, 137719, - 172546, 172295, 172150, 161148, 179199, 161803, - 155398, 186862, 180456, 179694, 211174, 15009, - 128375, 129121, 19914, 43692, 27387, 40653, - 45942, 40532, 34475, 41935, 45868, 39940, - 33825, 41934, 42153, 43524, 43342, 39930, - 36652, 38155, 36423, 39787, 38503, 35477944, - 171824}; - for (auto i = 0; i < 85; ++i) { - // mrefDbPtrs.emplace_back(CHRSIZES[i] + 1, nullptr); - mrefDb.emplace_back(CHRSIZES[i] + 1, MrefEntry{}); + DEFAULTREADLENGTH{defaultReadLengthIn}, + mrefDb{} { + + const vector &chrSizes = + GlobalAppConfig::getInstance().getChrConverter().chrSizesCompressedMref; + for (std::vector::size_type i = 0; i < chrSizes.size(); ++i) { + // mrefDbPtrs.emplace_back(chrSizes[i] + 1, nullptr); + mrefDb.emplace_back(chrSizes[i] + 1, MrefEntry{}); } vector header{"#chr", "start", "end"}; for (const auto &gzFile : filesIn) { @@ -108,6 +97,7 @@ MasterRefProcessor::MasterRefProcessor(const vector &filesIn, } auto defuzzier = DeFuzzier{DEFAULTREADLENGTH * 3, true}; auto i = 84; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); while (!mrefDb.empty()) { mrefDb.back().erase( remove_if(mrefDb.back().begin(), mrefDb.back().end(), @@ -118,7 +108,7 @@ MasterRefProcessor::MasterRefProcessor(const vector &filesIn, remove_if(mrefDb.back().begin(), mrefDb.back().end(), [](const MrefEntry &bp) { return bp.getPos() == -1; }), mrefDb.back().end()); - auto chromosome = ChrConverter::indexToChrCompressedMref[i]; + auto chromosome = chrConverter.indexToChrCompressedMref[i]; --i; for (auto &bp : mrefDb.back()) { if (bp.getPos() != -1 && bp.getValidityScore() != -1) { @@ -141,11 +131,12 @@ MasterRefProcessor::processFile(const string &gzPath, short fileIndex) { string sophiaLine{}; vector> fileBps{85, vector{}}; auto lineIndex = 0; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); while (error_terminating_getline(gzStream, sophiaLine)) { if (sophiaLine[0] != '#') { auto chrIndex = - ChrConverter::indexConverter[ChrConverter::readChromosomeIndex( - sophiaLine.cbegin(), '\t')]; + chrConverter.indexConverter[chrConverter.readChromosomeIndex( + sophiaLine.cbegin(), '\t')]; if (chrIndex < 0) { continue; } diff --git a/src/MrefEntry.cpp b/src/MrefEntry.cpp index 7db1c71..11405f1 100644 --- a/src/MrefEntry.cpp +++ b/src/MrefEntry.cpp @@ -22,6 +22,7 @@ #include "Breakpoint.h" #include "strtk.hpp" +#include "GlobalAppConfig.h" #include #include #include @@ -30,7 +31,7 @@ namespace sophia { - using namespace std; +using namespace std; boost::format MrefEntry::doubleFormatter { "%.5f" }; int MrefEntry::NUMPIDS { }; @@ -52,8 +53,12 @@ void MrefEntry::addEntry(BreakpointReduced& tmpBreakpoint, int fileIndex) { auto eventTotal = tmpBreakpoint.getPairedBreaksSoft() + tmpBreakpoint.getPairedBreaksHard() + tmpBreakpoint.getUnpairedBreaksSoft() + tmpBreakpoint.getUnpairedBreaksHard() + tmpBreakpoint.getBreaksShortIndel(); auto breakTotal = eventTotal + artifactBreakTotal; if (breakTotal < 200) { + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); for (auto saPtr : tmpBreakpoint.getSupplementsPtr()) { - if (saPtr->isSuspicious() || saPtr->isToRemove() || (saPtr->getChrIndex() != 1001 && ChrConverter::indexConverter[saPtr->getChrIndex()] < 0)) { + if (saPtr->isSuspicious() + || saPtr->isToRemove() + || (saPtr->getChrIndex() != 1001 + && chrConverter.indexConverter[saPtr->getChrIndex()] < 0)) { continue; } auto qualCheck = false; diff --git a/src/MrefEntryAnno.cpp b/src/MrefEntryAnno.cpp index ed1060f..c1afb19 100644 --- a/src/MrefEntryAnno.cpp +++ b/src/MrefEntryAnno.cpp @@ -25,7 +25,6 @@ #include #include #include -#include "ChrConverter.h" namespace sophia { using namespace std; diff --git a/src/SuppAlignment.cpp b/src/SuppAlignment.cpp index 694a640..3fabcee 100644 --- a/src/SuppAlignment.cpp +++ b/src/SuppAlignment.cpp @@ -25,17 +25,27 @@ #include #include "strtk.hpp" #include "ChrConverter.h" +#include "GlobalAppConfig.h" //#include namespace sophia { - using namespace std; +using namespace std; double SuppAlignment::ISIZEMAX { }; int SuppAlignment::DEFAULTREADLENGTH { }; -SuppAlignment::SuppAlignment(string::const_iterator saCbegin, string::const_iterator saCend, bool primaryIn, bool lowMapqSourceIn, bool nullMapqSourceIn, bool alignmentOnForwardStrand, bool bpEncounteredM, int originIndexIn, int bpChrIndex, int bpPos) : +SuppAlignment::SuppAlignment(string::const_iterator saCbegin, + string::const_iterator saCend, + bool primaryIn, + bool lowMapqSourceIn, + bool nullMapqSourceIn, + bool alignmentOnForwardStrand, + bool bpEncounteredM, + int originIndexIn, + int bpChrIndex, + int bpPos) : matchFuzziness { 5 * DEFAULTREADLENGTH }, chrIndex { 0 }, pos { 0 }, @@ -71,6 +81,7 @@ SuppAlignment::SuppAlignment(string::const_iterator saCbegin, string::const_iter // cerr << *cigarString_cit; // } // cerr << endl; + vector fieldBegins = { saCbegin }; vector fieldEnds; for (auto it = saCbegin; it != saCend; ++it) { @@ -80,14 +91,17 @@ SuppAlignment::SuppAlignment(string::const_iterator saCbegin, string::const_iter } } fieldEnds.push_back(saCend); - chrIndex = ChrConverter::readChromosomeIndex(fieldBegins[0], ','); + + chrIndex = GlobalAppConfig::getInstance(). + getChrConverter().readChromosomeIndex(fieldBegins[0], ','); if (chrIndex > 1001) { return; } for (auto it = fieldBegins[1]; it != fieldEnds[1]; ++it) { pos = 10 * pos + (*it - '0'); } -//cerr << "guessSupplementOffset" << endl; + + //cerr << "guessSupplementOffset" << endl; vector cigarChunks; auto cigarEncounteredM = false; auto cumulativeNucleotideCount = 0, currentNucleotideCount = 0, chunkIndex = 0, bestChunkIndex = 0, indelAdjustment = 0; @@ -106,7 +120,10 @@ SuppAlignment::SuppAlignment(string::const_iterator saCbegin, string::const_iter if (!cigarEncounteredM) { leftClipAdjustment = currentNucleotideCount; } - cigarChunks.emplace_back(*cigarString_cit, cigarEncounteredM, cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, currentNucleotideCount); + cigarChunks.emplace_back(*cigarString_cit, + cigarEncounteredM, + cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, + currentNucleotideCount); if (largestClip < currentNucleotideCount) { largestClip = currentNucleotideCount; bestChunkIndex = chunkIndex; @@ -115,7 +132,10 @@ SuppAlignment::SuppAlignment(string::const_iterator saCbegin, string::const_iter cumulativeNucleotideCount += currentNucleotideCount; break; case 'H': - cigarChunks.emplace_back(*cigarString_cit, cigarEncounteredM, cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, currentNucleotideCount); + cigarChunks.emplace_back(*cigarString_cit, + cigarEncounteredM, + cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, + currentNucleotideCount); if (largestClip < currentNucleotideCount) { largestClip = currentNucleotideCount; bestChunkIndex = chunkIndex; @@ -159,12 +179,24 @@ void SuppAlignment::finalizeSupportingIndices() { sort(supportingIndices.begin(), supportingIndices.end()); sort(supportingIndicesSecondary.begin(), supportingIndicesSecondary.end()); supportingIndices.erase(unique(supportingIndices.begin(), supportingIndices.end()), supportingIndices.end()); - supportingIndicesSecondary.erase(unique(supportingIndicesSecondary.begin(), supportingIndicesSecondary.end()), supportingIndicesSecondary.end()); + supportingIndicesSecondary.erase(unique(supportingIndicesSecondary.begin(), + supportingIndicesSecondary.end()), + supportingIndicesSecondary.end()); support = static_cast(supportingIndices.size()); secondarySupport = static_cast(supportingIndicesSecondary.size()); } -SuppAlignment::SuppAlignment(int chrIndexIn, int posIn, int mateSupportIn, int expectedDiscordantsIn, bool encounteredMIn, bool invertedIn, int extendedPosIn, bool primaryIn, bool lowMapqSourceIn, bool nullMapqSourceIn, int originIndexIn) : +SuppAlignment::SuppAlignment(int chrIndexIn, + int posIn, + int mateSupportIn, + int expectedDiscordantsIn, + bool encounteredMIn, + bool invertedIn, + int extendedPosIn, + bool primaryIn, + bool lowMapqSourceIn, + bool nullMapqSourceIn, + int originIndexIn) : matchFuzziness { 5 * DEFAULTREADLENGTH }, chrIndex { chrIndexIn }, pos { posIn }, @@ -213,12 +245,27 @@ string SuppAlignment::print() const { } else { invStr.append("|"); } + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); if (!fuzzy) { - outStr.append(ChrConverter::indexToChr[chrIndex]).append(":").append(strtk::type_to_string(pos)); + outStr. + append(chrConverter.indexToChr[chrIndex]). + append(":"). + append(strtk::type_to_string(pos)); } else { - outStr.append(ChrConverter::indexToChr[chrIndex]).append(":").append(strtk::type_to_string(pos)).append("-").append(strtk::type_to_string(extendedPos)); + outStr. + append(chrConverter.indexToChr[chrIndex]). + append(":"). + append(strtk::type_to_string(pos)). + append("-"). + append(strtk::type_to_string(extendedPos)); } - outStr.append(invStr).append("(").append(strtk::type_to_string(support)).append(",").append(strtk::type_to_string(secondarySupport)).append(","); + outStr. + append(invStr). + append("("). + append(strtk::type_to_string(support)). + append(","). + append(strtk::type_to_string(secondarySupport)). + append(","); if (!suspicious) { outStr.append(strtk::type_to_string(mateSupport)); if (semiSuspicious || nullMapqSource) { @@ -261,7 +308,8 @@ SuppAlignment::SuppAlignment(const string& saIn) : if (encounteredM) { ++index; } - chrIndex = ChrConverter::readChromosomeIndex(next(saIn.cbegin(), index), ':'); + chrIndex = GlobalAppConfig::getInstance(). + getChrConverter().readChromosomeIndex(next(saIn.cbegin(), index), ':'); if (chrIndex > 1001) { return; } @@ -322,7 +370,8 @@ bool SuppAlignment::saCloseness(const SuppAlignment& rhs, int fuzziness) const { if (inverted == rhs.isInverted() && chrIndex == rhs.getChrIndex() && encounteredM == rhs.isEncounteredM()) { if (strictFuzzy || rhs.isStrictFuzzy()) { fuzziness = 2.5 * DEFAULTREADLENGTH; - return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); + return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && + (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); } else { return abs(pos - rhs.getPos()) <= fuzziness; } @@ -338,7 +387,8 @@ bool SuppAlignment::saDistHomologyRescueCloseness(const SuppAlignment& rhs, int } if (chrIndex == rhs.getChrIndex() && encounteredM == rhs.isEncounteredM()) { if (strictFuzzy || rhs.isStrictFuzzy()) { - return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); + return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && + (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); } else { return abs(pos - rhs.getPos()) <= fuzziness; } diff --git a/src/SuppAlignmentAnno.cpp b/src/SuppAlignmentAnno.cpp index f823061..9794157 100644 --- a/src/SuppAlignmentAnno.cpp +++ b/src/SuppAlignmentAnno.cpp @@ -23,6 +23,7 @@ */ #include "SuppAlignmentAnno.h" +#include "GlobalAppConfig.h" #include "ChrConverter.h" #include "strtk.hpp" #include @@ -49,7 +50,8 @@ SuppAlignmentAnno::SuppAlignmentAnno(const string &saStrIn) ++index; } chrIndex = - ChrConverter::readChromosomeIndex(next(saStrIn.cbegin(), index), ':'); + GlobalAppConfig::getInstance(). + getChrConverter().readChromosomeIndex(next(saStrIn.cbegin(), index), ':'); if (chrIndex > 1001) { return; } @@ -178,12 +180,13 @@ SuppAlignmentAnno::print() const { } else { invStr.append("|"); } + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); if (!fuzzy || pos == extendedPos) { - outStr.append(ChrConverter::indexToChr[chrIndex]) + outStr.append(chrConverter.indexToChr[chrIndex]) .append(":") .append(strtk::type_to_string(pos)); } else { - outStr.append(ChrConverter::indexToChr[chrIndex]) + outStr.append(chrConverter.indexToChr[chrIndex]) .append(":") .append(strtk::type_to_string(pos)) .append("-") diff --git a/src/SvEvent.cpp b/src/SvEvent.cpp index 68cae30..1e7e3b7 100644 --- a/src/SvEvent.cpp +++ b/src/SvEvent.cpp @@ -23,6 +23,7 @@ */ #include "ChrConverter.h" +#include "GlobalAppConfig.h" #include "strtk.hpp" #include @@ -139,10 +140,11 @@ SvEvent::SvEvent(const BreakpointReduced &bp1In, const BreakpointReduced &bp2In, determineGermlineClonalityBp(bp2In, selectedSa2, germlineClonality2); germlineStatus2 = germlineClonality2 > 0.15; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && !selectedSa2.isProperPairErrorProne() && - ChrConverter::indexConverter[chrIndex1] < 23 && - ChrConverter::indexConverter[chrIndex2] < 23; + chrConverter.indexConverter[chrIndex1] < 23 && + chrConverter.indexConverter[chrIndex2] < 23; auto splitSupportThreshold1 = (strictNonDecoy && !selectedSa1.isSemiSuspicious() && (mateRatio1 >= 0.6)) @@ -336,9 +338,10 @@ SvEvent::SvEvent(const BreakpointReduced &bp1In, const BreakpointReduced &bp2In, determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); germlineStatus1 = germlineClonality1 > 0.15; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && - ChrConverter::indexConverter[chrIndex1] < 23 && - ChrConverter::indexConverter[chrIndex2] < 23; + chrConverter.indexConverter[chrIndex1] < 23 && + chrConverter.indexConverter[chrIndex2] < 23; auto splitSupportThreshold = (strictNonDecoy && !selectedSa2.isSemiSuspicious() && (mateRatio1 >= 0.66)) @@ -531,9 +534,10 @@ SvEvent::SvEvent(const BreakpointReduced &bp1In, const SuppAlignmentAnno &sa1In, determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); germlineStatus1 = germlineClonality1 > 0.15; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && - ChrConverter::indexConverter[chrIndex1] < 23 && - ChrConverter::indexConverter[chrIndex2] < 23; + chrConverter.indexConverter[chrIndex1] < 23 && + chrConverter.indexConverter[chrIndex2] < 23; auto splitSupportThreshold = (strictNonDecoy && (mateRatio1 >= 0.66) ? 0 : 2); @@ -1705,10 +1709,11 @@ string SvEvent::printMatch(const vector> &overhangDb) const { vector outputFields; outputFields.reserve(20); - outputFields.emplace_back(ChrConverter::indexToChr[chrIndex1]); + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + outputFields.emplace_back(chrConverter.indexToChr[chrIndex1]); outputFields.emplace_back(strtk::type_to_string(pos1 - 1)); outputFields.emplace_back(strtk::type_to_string(pos1)); - outputFields.emplace_back(ChrConverter::indexToChr[chrIndex2]); + outputFields.emplace_back(chrConverter.indexToChr[chrIndex2]); outputFields.emplace_back(strtk::type_to_string(pos2 - 1)); outputFields.emplace_back( inputScore > 0 diff --git a/sophia.cpp b/src/sophia.cpp similarity index 57% rename from sophia.cpp rename to src/sophia.cpp index 5afecba..f8e4a17 100644 --- a/sophia.cpp +++ b/src/sophia.cpp @@ -10,21 +10,30 @@ #include #include #include +#include #include "Alignment.h" #include "SuppAlignment.h" #include "Breakpoint.h" #include "SamSegmentMapper.h" -#include "ChrConverter.h" #include "HelperFunctions.h" +#include "ChrConverter.h" +#include "Hg37ChrConverter.h" +#include "Hg38ChrConverter.h" +#include "GlobalAppConfig.h" + -std::pair getIsizeParameters(const std::string &ISIZEFILE); +using namespace std; +using namespace sophia; + +pair getIsizeParameters(const string &ISIZEFILE); int main(int argc, char** argv) { - std::ios_base::sync_with_stdio(false); - std::cin.tie(nullptr); + ios_base::sync_with_stdio(false); + cin.tie(nullptr); boost::program_options::options_description desc("Allowed options"); desc.add_options() // ("help", "produce help message") // - ("mergedisizes", boost::program_options::value(), "insertsize distribution file for the merged bam") // + ("assemblyname", boost::program_options::value(), "assembly name (hg37, hg38)") // + ("mergedisizes", boost::program_options::value(), "insertsize distribution file for the merged bam") // ("medianisize", boost::program_options::value(), "median insert size for the merged bam") // ("stdisizepercentage", boost::program_options::value(), "percentage standard deviation of the insert size for the merged bam") // ("defaultreadlength", boost::program_options::value(), "Default read length for the technology used in sequencing 101,151 etc.") // @@ -35,89 +44,120 @@ int main(int argc, char** argv) { ("isizesigma", boost::program_options::value(), "The number of sds a s's mate has to be away to be called as discordant. (5)") // ("bpsupport", boost::program_options::value(), "Minimum number of reads supporting a discordant contig. (5)") // ("properpairpercentage", boost::program_options::value(), "Proper pair ratio as a percentage (100.0)"); + boost::program_options::variables_map inputVariables { }; boost::program_options::store(boost::program_options::parse_command_line(argc, argv, desc), inputVariables); boost::program_options::notify(inputVariables); + if (inputVariables.count("help")) { - std::cout << desc << std::endl; + cout << desc << endl; return 0; } + int defaultReadLength { 0 }; if (inputVariables.count("defaultreadlength")) { defaultReadLength = inputVariables["defaultreadlength"].as(); } else { - std::cerr << "Default read Length not given, exiting" << std::endl; + cerr << "Default read Length not given, exiting" << endl; return 1; } + auto clipSize = 10, baseQuality = 23, baseQualityLow = 12, lowQualClipSize = 5, isizeSigmaLevel = 5; auto bpSupport = 5; auto properPairRatio = 1.0; + if (inputVariables.count("clipsize")) { clipSize = inputVariables["clipsize"].as(); } + if (inputVariables.count("basequality")) { baseQuality = inputVariables["basequality"].as(); } + if (inputVariables.count("basequalitylow")) { baseQualityLow = inputVariables["basequalitylow"].as(); } + if (inputVariables.count("lowqualclipsize")) { lowQualClipSize = inputVariables["lowqualclipsize"].as(); } + if (inputVariables.count("isizesigma")) { isizeSigmaLevel = inputVariables["isizesigma"].as(); } + if (inputVariables.count("bpsupport")) { bpSupport = inputVariables["bpsupport"].as(); } + if (inputVariables.count("properpairpercentage")) { properPairRatio = inputVariables["properpairpercentage"].as(); properPairRatio /= 100; if (properPairRatio < 0.9) { - sophia::Breakpoint::PROPERPAIRCOMPENSATIONMODE = true; - sophia::Breakpoint::IMPROPERPAIRRATIO = 0.9 - properPairRatio; + Breakpoint::PROPERPAIRCOMPENSATIONMODE = true; + Breakpoint::IMPROPERPAIRRATIO = 0.9 - properPairRatio; } } - std::string mergedIsizeFile; + + string mergedIsizeFile; if (inputVariables.count("mergedisizes")) { - mergedIsizeFile = inputVariables["mergedisizes"].as(); + mergedIsizeFile = inputVariables["mergedisizes"].as(); auto isizeparams = getIsizeParameters(mergedIsizeFile); - sophia::Alignment::ISIZEMAX = std::min(4000.0, isizeparams.first + isizeSigmaLevel * isizeparams.second); - sophia::SuppAlignment::ISIZEMAX = sophia::Alignment::ISIZEMAX; + Alignment::ISIZEMAX = min(4000.0, isizeparams.first + isizeSigmaLevel * isizeparams.second); + SuppAlignment::ISIZEMAX = Alignment::ISIZEMAX; } else { if (inputVariables.count("medianisize") && inputVariables.count("stdisizepercentage")) { auto medianIsize = inputVariables["medianisize"].as(); auto isizeStdPercentage = inputVariables["stdisizepercentage"].as(); - sophia::Alignment::ISIZEMAX = std::min(4000.0, medianIsize + isizeSigmaLevel * medianIsize * isizeStdPercentage * 0.01); - sophia::SuppAlignment::ISIZEMAX = sophia::Alignment::ISIZEMAX; + Alignment::ISIZEMAX = min(4000.0, medianIsize + isizeSigmaLevel * medianIsize * isizeStdPercentage * 0.01); + SuppAlignment::ISIZEMAX = Alignment::ISIZEMAX; } else { - sophia::Alignment::ISIZEMAX = 2000.0; - sophia::SuppAlignment::ISIZEMAX = 2000.0; - std::cerr << "No insert size distribution file given, using a dummy default value of 2000 as the min insert size of a distant event" << std::endl; + Alignment::ISIZEMAX = 2000.0; + SuppAlignment::ISIZEMAX = 2000.0; + cerr << "No insert size distribution file given, using a dummy default value of 2000 as the min insert size of a distant event" << endl; } } - sophia::Alignment::CLIPPEDNUCLEOTIDECOUNTTHRESHOLD = clipSize; - sophia::Alignment::BASEQUALITYTHRESHOLD = baseQuality + 33; - sophia::Alignment::BASEQUALITYTHRESHOLDLOW = baseQualityLow + 33; - sophia::Alignment::LOWQUALCLIPTHRESHOLD = lowQualClipSize; - sophia::Breakpoint::BPSUPPORTTHRESHOLD = bpSupport; - sophia::Breakpoint::DEFAULTREADLENGTH = defaultReadLength; - sophia::Breakpoint::DISCORDANTLOWQUALLEFTRANGE = static_cast(std::round(defaultReadLength * 1.11)); - sophia::Breakpoint::DISCORDANTLOWQUALRIGHTRANGE = static_cast(std::round(defaultReadLength * 0.51)); - - sophia::SuppAlignment::DEFAULTREADLENGTH = defaultReadLength; - sophia::ChosenBp::BPSUPPORTTHRESHOLD = bpSupport; - std::cout << sophia::Breakpoint::COLUMNSSTR; - sophia::SamSegmentMapper segmentRefMaster { defaultReadLength }; + + unique_ptr chrConverter; + if (!inputVariables.count("assemblyname") || + inputVariables["assemblyname"].as() == Hg37ChrConverter::assembly_name) { + chrConverter = unique_ptr(new Hg37ChrConverter()); + } else if (inputVariables["assemblyname"].as() == Hg38ChrConverter::assembly_name) { + chrConverter = unique_ptr(new Hg38ChrConverter()); + } else { + cerr << "Unknown assembly name " << inputVariables["assemblyname"].as() << ". I know " + << Hg37ChrConverter::assembly_name << " and " + << Hg38ChrConverter::assembly_name << endl; + return 1; + } + + // Initialize the global application configuration. + GlobalAppConfig::init(move(chrConverter)); + + Alignment::CLIPPEDNUCLEOTIDECOUNTTHRESHOLD = clipSize; + Alignment::BASEQUALITYTHRESHOLD = baseQuality + 33; + Alignment::BASEQUALITYTHRESHOLDLOW = baseQualityLow + 33; + Alignment::LOWQUALCLIPTHRESHOLD = lowQualClipSize; + Breakpoint::BPSUPPORTTHRESHOLD = bpSupport; + Breakpoint::DEFAULTREADLENGTH = defaultReadLength; + Breakpoint::DISCORDANTLOWQUALLEFTRANGE = static_cast(round(defaultReadLength * 1.11)); + Breakpoint::DISCORDANTLOWQUALRIGHTRANGE = static_cast(round(defaultReadLength * 0.51)); + + SuppAlignment::DEFAULTREADLENGTH = defaultReadLength; + ChosenBp::BPSUPPORTTHRESHOLD = bpSupport; + cout << Breakpoint::COLUMNSSTR; + SamSegmentMapper segmentRefMaster { defaultReadLength }; segmentRefMaster.parseSamStream(); + return 0; } -std::pair getIsizeParameters(const std::string &ISIZEFILE) { - std::pair isizeMedianStd { }; - std::ifstream infile { ISIZEFILE }; - std::string line; + +pair getIsizeParameters(const string &ISIZEFILE) { + pair isizeMedianStd { }; + ifstream infile { ISIZEFILE }; + string line; auto i = 0; - while (sophia::error_terminating_getline(infile, line)) { + while (error_terminating_getline(infile, line)) { boost::algorithm::trim_right(line); switch (i) { case 0: diff --git a/sophiaAnnotate.cpp b/src/sophiaAnnotate.cpp similarity index 62% rename from sophiaAnnotate.cpp rename to src/sophiaAnnotate.cpp index da1f2f5..0c6ca29 100644 --- a/sophiaAnnotate.cpp +++ b/src/sophiaAnnotate.cpp @@ -7,6 +7,7 @@ #include #include #include +#include #include "cxxopts.hpp" #include "BreakpointReduced.h" #include "AnnotationProcessor.h" @@ -19,12 +20,16 @@ #include "MrefEntryAnno.h" #include #include -#include "ChrConverter.h" #include "HelperFunctions.h" +#include "ChrConverter.h" +#include "Hg37ChrConverter.h" +#include "Hg38ChrConverter.h" +#include "GlobalAppConfig.h" int main(int argc, char** argv) { using namespace std; + using namespace sophia; ios_base::sync_with_stdio(false); cin.tie(nullptr); @@ -32,6 +37,7 @@ int main(int argc, char** argv) { options.add_options() // ("help", "produce help message") // ("mref", "mref file", cxxopts::value()) // + ("assemblyname", "assembly name", cxxopts::value()) // ("tumorresults", "_bps.bed.gz file from sophia for the tumor, or control for a no-tumor analysis", cxxopts::value()) // ("controlresults", "_bps.bed.gz file from sophia for the control", cxxopts::value()) // ("defaultreadlengthtumor", "Default read length for the technology used in sequencing 101,151 etc., tumor", cxxopts::value()) // @@ -46,8 +52,10 @@ int main(int argc, char** argv) { ("germlineoffset", "Minimum offset a germline bp and a control bp. (5)", cxxopts::value()) // ("germlinedblimit", "Maximum occurrence of germline variants in the db. (5)", cxxopts::value()) // ("debugmode", "debugmode"); + options.parse(argc, argv); - vector> mref { 85, vector { } }; + + vector> mref { 85, vector { } }; if (!options.count("mref")) { cerr << "No mref file given, exiting" << endl; return 1; @@ -60,13 +68,16 @@ int main(int argc, char** argv) { cerr << "No input file given, exiting" << endl; return 1; } + int pidsInMref { 0 }; + if (options.count("pidsinmref")) { pidsInMref = options["pidsinmref"].as(); } else { cerr << "number of PIDS in the MREF not given, exiting" << endl; return 1; } + int defaultReadLengthTumor { 0 }; if (options.count("defaultreadlengthtumor")) { defaultReadLengthTumor = options["defaultreadlengthtumor"].as(); @@ -74,81 +85,109 @@ int main(int argc, char** argv) { cerr << "Default read Length not given, exiting" << endl; return 1; } + int artifactlofreq { 33 }; if (options.count("artifactlofreq")) { artifactlofreq = options["artifactlofreq"].as(); } + int artifacthifreq { 50 }; if (options.count("artifacthifreq")) { artifacthifreq = options["artifacthifreq"].as(); } + int clonalitylofreq { 5 }; if (options.count("clonalitylofreq")) { clonalitylofreq = options["clonalitylofreq"].as(); } + int clonalitystrictlofreq { 20 }; if (options.count("clonalitystrictlofreq")) { clonalitystrictlofreq = options["clonalitystrictlofreq"].as(); } + int clonalityhifreq { 85 }; if (options.count("clonalityhifreq")) { clonalityhifreq = options["clonalityhifreq"].as(); } + int bpFreq { 3 }; if (options.count("bpfreq")) { bpFreq = options["bpfreq"].as(); } + int germlineOffset { 5 }; if (options.count("germlineoffset")) { germlineOffset = options["germlineoffset"].as(); } + int germlineDbLimit { 5 }; if (options.count("germlinedblimit")) { germlineDbLimit = options["germlinedblimit"].as(); } - sophia::MrefEntryAnno::PIDSINMREF = pidsInMref; + + unique_ptr chrConverter; + if (!options.count("assemblyname") || + options["assemblyname"].as() == Hg37ChrConverter::assembly_name) { + chrConverter = unique_ptr(new Hg37ChrConverter()); + } else if (options["assemblyname"].as() == Hg38ChrConverter::assembly_name) { + chrConverter = unique_ptr(new Hg38ChrConverter()); + } else { + cerr << "Unknown assembly name " << options["assemblyname"].as() << ". I know " + << Hg37ChrConverter::assembly_name << " and " + << Hg38ChrConverter::assembly_name << endl; + return 1; + } + + // Initialize global application config. + const GlobalAppConfig &config = GlobalAppConfig::init(move(chrConverter)); + + MrefEntryAnno::PIDSINMREF = pidsInMref; unique_ptr mrefInputHandle { make_unique(options["mref"].as(), ios_base::in | ios_base::binary) }; unique_ptr mrefGzHandle { make_unique() }; mrefGzHandle->push(boost::iostreams::gzip_decompressor()); mrefGzHandle->push(*mrefInputHandle); cerr << "m\n"; string line { }; - while (sophia::error_terminating_getline(*mrefGzHandle, line)) { + + const ChrConverter &chrConverterTmp = config.getChrConverter(); + while (error_terminating_getline(*mrefGzHandle, line)) { if (line.front() == '#') { continue; }; - auto chrIndex = sophia::ChrConverter::indexConverter[sophia::ChrConverter::readChromosomeIndex(line.cbegin(), '\t')]; + auto chrIndex = + chrConverterTmp.indexConverter[chrConverterTmp.readChromosomeIndex(line.cbegin(), '\t')]; if (chrIndex < 0) { continue; } mref[chrIndex].emplace_back(line); } - sophia::SvEvent::ARTIFACTFREQLOWTHRESHOLD = (artifactlofreq + 0.0) / 100; - sophia::SvEvent::ARTIFACTFREQHIGHTHRESHOLD = (artifacthifreq + 0.0) / 100; - sophia::BreakpointReduced::ARTIFACTFREQHIGHTHRESHOLD = sophia::SvEvent::ARTIFACTFREQHIGHTHRESHOLD; - sophia::SvEvent::CLONALITYLOWTHRESHOLD = (clonalitylofreq + 0.0) / 100; - sophia::SvEvent::CLONALITYSTRICTLOWTHRESHOLD = (clonalitystrictlofreq + 0.0) / 100; - sophia::BreakpointReduced::CLONALITYSTRICTLOWTHRESHOLD = sophia::SvEvent::CLONALITYSTRICTLOWTHRESHOLD; - sophia::SvEvent::CLONALITYHIGHTHRESHOLD = (clonalityhifreq + 0.0) / 100; - sophia::SvEvent::BPFREQTHRESHOLD = pidsInMref * (bpFreq + 0.0) / 100; - sophia::SvEvent::RELAXEDBPFREQTHRESHOLD = 3 * sophia::SvEvent::BPFREQTHRESHOLD; - sophia::SvEvent::PIDSINMREFSTR = strtk::type_to_string(pidsInMref); - sophia::BreakpointReduced::PIDSINMREFSTR = sophia::SvEvent::PIDSINMREFSTR; - sophia::BreakpointReduced::DEFAULTREADLENGTH = defaultReadLengthTumor; - sophia::Breakpoint::DEFAULTREADLENGTH = defaultReadLengthTumor; - sophia::SuppAlignment::DEFAULTREADLENGTH = defaultReadLengthTumor; - sophia::SuppAlignmentAnno::DEFAULTREADLENGTH = defaultReadLengthTumor; - sophia::SvEvent::HALFDEFAULTREADLENGTH = round(defaultReadLengthTumor / 2.0); - sophia::SvEvent::GERMLINEOFFSETTHRESHOLD = germlineOffset; - sophia::SvEvent::GERMLINEDBLIMIT = germlineDbLimit; - sophia::SvEvent::ABRIDGEDOUTPUT = true; + SvEvent::ARTIFACTFREQLOWTHRESHOLD = (artifactlofreq + 0.0) / 100; + SvEvent::ARTIFACTFREQHIGHTHRESHOLD = (artifacthifreq + 0.0) / 100; + BreakpointReduced::ARTIFACTFREQHIGHTHRESHOLD = SvEvent::ARTIFACTFREQHIGHTHRESHOLD; + SvEvent::CLONALITYLOWTHRESHOLD = (clonalitylofreq + 0.0) / 100; + SvEvent::CLONALITYSTRICTLOWTHRESHOLD = (clonalitystrictlofreq + 0.0) / 100; + BreakpointReduced::CLONALITYSTRICTLOWTHRESHOLD = SvEvent::CLONALITYSTRICTLOWTHRESHOLD; + SvEvent::CLONALITYHIGHTHRESHOLD = (clonalityhifreq + 0.0) / 100; + SvEvent::BPFREQTHRESHOLD = pidsInMref * (bpFreq + 0.0) / 100; + SvEvent::RELAXEDBPFREQTHRESHOLD = 3 * SvEvent::BPFREQTHRESHOLD; + SvEvent::PIDSINMREFSTR = strtk::type_to_string(pidsInMref); + BreakpointReduced::PIDSINMREFSTR = SvEvent::PIDSINMREFSTR; + BreakpointReduced::DEFAULTREADLENGTH = defaultReadLengthTumor; + Breakpoint::DEFAULTREADLENGTH = defaultReadLengthTumor; + SuppAlignment::DEFAULTREADLENGTH = defaultReadLengthTumor; + SuppAlignmentAnno::DEFAULTREADLENGTH = defaultReadLengthTumor; + SvEvent::HALFDEFAULTREADLENGTH = round(defaultReadLengthTumor / 2.0); + SvEvent::GERMLINEOFFSETTHRESHOLD = germlineOffset; + SvEvent::GERMLINEDBLIMIT = germlineDbLimit; + SvEvent::ABRIDGEDOUTPUT = true; if (options.count("debugmode")) { - sophia::SvEvent::DEBUGMODE = true; + SvEvent::DEBUGMODE = true; } else { - sophia::SvEvent::DEBUGMODE = false; + SvEvent::DEBUGMODE = false; } - sophia::AnnotationProcessor::ABRIDGEDOUTPUT = true; - sophia::Breakpoint::BPSUPPORTTHRESHOLD = 3; + AnnotationProcessor::ABRIDGEDOUTPUT = true; + Breakpoint::BPSUPPORTTHRESHOLD = 3; if (options.count("controlresults")) { string controlResults { options["controlresults"].as() }; int defaultReadLengthControl { 0 }; @@ -161,18 +200,19 @@ int main(int argc, char** argv) { auto lowQualControl = 0; auto pathogenInControl = false; { - sophia::SvEvent::NOCONTROLMODE = true; - sophia::AnnotationProcessor annotationProcessorControlCheck { controlResults, mref, defaultReadLengthControl, true, germlineDbLimit }; + SvEvent::NOCONTROLMODE = true; + AnnotationProcessor annotationProcessorControlCheck { controlResults, mref, defaultReadLengthControl, true, germlineDbLimit }; lowQualControl = annotationProcessorControlCheck.getMassiveInvFilteringLevel(); pathogenInControl = annotationProcessorControlCheck.isContaminationObserved(); - sophia::SvEvent::NOCONTROLMODE = false; + SvEvent::NOCONTROLMODE = false; } - sophia::AnnotationProcessor annotationProcessor { tumorResults, mref, controlResults, defaultReadLengthTumor, defaultReadLengthControl, germlineDbLimit, lowQualControl, pathogenInControl }; + AnnotationProcessor annotationProcessor { tumorResults, mref, controlResults, defaultReadLengthTumor, defaultReadLengthControl, germlineDbLimit, lowQualControl, pathogenInControl }; annotationProcessor.printFilteredResults(pathogenInControl, lowQualControl); } else { - sophia::SvEvent::NOCONTROLMODE = true; - sophia::AnnotationProcessor annotationProcessor { tumorResults, mref, defaultReadLengthTumor, false, germlineDbLimit }; + SvEvent::NOCONTROLMODE = true; + AnnotationProcessor annotationProcessor { tumorResults, mref, defaultReadLengthTumor, false, germlineDbLimit }; annotationProcessor.printFilteredResults(false, 0); } + return 0; } diff --git a/sophiaMref.cpp b/src/sophiaMref.cpp similarity index 51% rename from sophiaMref.cpp rename to src/sophiaMref.cpp index 15cbb32..67dfce2 100644 --- a/sophiaMref.cpp +++ b/src/sophiaMref.cpp @@ -13,25 +13,39 @@ #include "MasterRefProcessor.h" #include "MrefEntry.h" #include "HelperFunctions.h" +#include "GlobalAppConfig.h" +#include "ChrConverter.h" +#include "Hg37ChrConverter.h" +#include "Hg38ChrConverter.h" +#include "HelperFunctions.h" int main(int argc, char** argv) { using namespace std; + using namespace sophia; boost::program_options::options_description desc("Allowed options"); desc.add_options() // ("help", "produce help message") // - ("gzins", boost::program_options::value(), "list of all gzipped control beds") // - ("version", boost::program_options::value(), "version") // - ("defaultreadlength", boost::program_options::value(), "Default read length for the technology used in sequencing 101,151 etc.") // - ("outputrootname", boost::program_options::value(), "outputrootname"); + ("assemblyname", boost::program_options::value(), "assembly name (hg37, hg38)") // + ("gzins", boost::program_options::value(), + "list of all gzipped control beds") // + ("version", boost::program_options::value(), + "version") // + ("defaultreadlength", boost::program_options::value(), + "Default read length for the technology used in sequencing 101,151 etc.") // + ("outputrootname", boost::program_options::value(), + "outputrootname"); + boost::program_options::variables_map inputVariables { }; boost::program_options::store(boost::program_options::parse_command_line(argc, argv, desc), inputVariables); boost::program_options::notify(inputVariables); + if (inputVariables.count("help")) { cout << desc << endl; return 0; } + string gzInFilesList; if (inputVariables.count("gzins")) { gzInFilesList = inputVariables["gzins"].as(); @@ -39,11 +53,13 @@ int main(int argc, char** argv) { cerr << "No gzipped control bed list file given, exiting" << endl; return 1; } + ifstream gzInFilesHandle { gzInFilesList }; vector gzListIn; for (string line; error_terminating_getline(gzInFilesHandle, line);) { gzListIn.push_back(line); } + string version { }; if (inputVariables.count("version")) { version = inputVariables["version"].as(); @@ -51,6 +67,7 @@ int main(int argc, char** argv) { cerr << "No input version given, exiting" << endl; return 1; } + int defaultReadLength { 0 }; if (inputVariables.count("defaultreadlength")) { defaultReadLength = inputVariables["defaultreadlength"].as(); @@ -58,6 +75,7 @@ int main(int argc, char** argv) { cerr << "Default read Length not given, exiting" << endl; return 1; } + string outputRoot { }; if (inputVariables.count("outputrootname")) { outputRoot = inputVariables["outputrootname"].as(); @@ -65,8 +83,27 @@ int main(int argc, char** argv) { cerr << "No output file root name given, exiting" << endl; return 1; } - sophia::SuppAlignment::DEFAULTREADLENGTH = defaultReadLength; - sophia::SuppAlignmentAnno::DEFAULTREADLENGTH = defaultReadLength; - sophia::MrefEntry::NUMPIDS = gzListIn.size(); - sophia::MasterRefProcessor mRefProcessor { gzListIn, outputRoot, version, defaultReadLength }; + + unique_ptr chrConverter; + if (!inputVariables.count("assemblyname") || + inputVariables["assemblyname"].as() == Hg37ChrConverter::assembly_name) { + chrConverter = unique_ptr(new Hg37ChrConverter()); + } else if (inputVariables["assemblyname"].as() == Hg38ChrConverter::assembly_name) { + chrConverter = unique_ptr(new Hg38ChrConverter()); + } else { + cerr << "Unknown assembly name " << inputVariables["assemblyname"].as() << ". I know " + << Hg37ChrConverter::assembly_name << " and " + << Hg38ChrConverter::assembly_name << endl; + return 1; + } + + // Initialize the global application configuration. + GlobalAppConfig::init(move(chrConverter)); + + SuppAlignment::DEFAULTREADLENGTH = defaultReadLength; + SuppAlignmentAnno::DEFAULTREADLENGTH = defaultReadLength; + MrefEntry::NUMPIDS = gzListIn.size(); + MasterRefProcessor mRefProcessor { gzListIn, outputRoot, version, defaultReadLength }; + + return 0; } From 703ef68a933df0d0751fcc1acb9c9c9af50dbb27 Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Wed, 13 Dec 2023 11:38:05 +0100 Subject: [PATCH 02/79] Build script for sophiaMref. Signed-off-by: Philip R. Kensche --- .gitignore | 1 + README.md | 16 +++++++- Release_sophiaMref/build-sophiaMref.sh | 56 ++++++++++++++++++++++++++ 3 files changed, 72 insertions(+), 1 deletion(-) create mode 100755 Release_sophiaMref/build-sophiaMref.sh diff --git a/.gitignore b/.gitignore index 2dc7a6e..2a7d3eb 100644 --- a/.gitignore +++ b/.gitignore @@ -3,4 +3,5 @@ *.o Release_sophia/sophia Release_sophiaAnnotate/sophiaAnnotate +Release_sophiaMref/sophiaMref include/strtk.hpp diff --git a/README.md b/README.md index 2e632ee..32d80be 100644 --- a/README.md +++ b/README.md @@ -41,7 +41,7 @@ Note that the build-scripts are for when you manage your dependencies with Conda ### Static Build -If you want to compile statically you need to install glibc and boost static libraries (not possible with Conda, in the moment) and do +If you want to compile statically you need to install glibc and boost static libraries (currently, not possible with Conda) and do ```bash source activate sophia @@ -52,3 +52,17 @@ STATIC=true build-sophia.sh cd ../Release_sophiaAnnotate STATIC=true build-sophiaAnnotate.sh ``` + +## Changes + +* 35.1.0 (upcoming) + * Minor: Nominally added support for hg38 (hg37 support remains) + * Minor: Added `--assemblyname` option, defaulting to "hg37" when omitted (old behaviour) + > WARNING: hg38 support was not excessively tested. In particular, yet hardcoded parameters may have to be adjusted. + * Minor: Build script for `sophiaMref` + * Patch: Code readability improvements, `.editorconfig` file, and `clang-format` configuration + * Patch: Improved compilation instructions + * Patch: Use `namespace::std` to get rid of `std::` noise in the code + +* 9e3b6ed + * Last version in [bitbucket]](https://bitbucket.org/compbio_charite/sophia/src/master/) \ No newline at end of file diff --git a/Release_sophiaMref/build-sophiaMref.sh b/Release_sophiaMref/build-sophiaMref.sh new file mode 100755 index 0000000..7bb4542 --- /dev/null +++ b/Release_sophiaMref/build-sophiaMref.sh @@ -0,0 +1,56 @@ +#!/bin/bash + +set -uex +trap 'echo "Compilation failed with an error" >> /dev/stderr' ERR + +CONDA_PREFIX="${CONDA_PREFIX:?No CONDA_PREFIX -- no active Conda environment}" + +install_strtk() { + wget -c https://github.com/ArashPartow/strtk/raw/master/strtk.hpp -O ../include/strtk.hpp +} + +install_strtk + +CPP=x86_64-conda_cos6-linux-gnu-g++ +INCLUDES="-I../include -I$CONDA_PREFIX/include" +CPP_OPTS="-L$CONDA_PREFIX/lib -std=c++17 $INCLUDES -O3 -Wall -Wextra -static -static-libgcc -static-libstdc++ -flto -c -fmessage-length=0 -Wno-attributes" + +if [[ "${STATIC:-false}" == "true" ]]; then + CPP_OPTS="-static -static-libgcc -static-libstdc++ $CPP_OPTS" +fi + +$CPP $CPP_OPTS -o "GlobalAppConfig.o" "../src/GlobalAppConfig.cpp" +$CPP $CPP_OPTS -o "ChrConverter.o" "../src/ChrConverter.cpp" +$CPP $CPP_OPTS -o "Hg37ChrConverter.o" "../src/Hg37ChrConverter.cpp" +$CPP $CPP_OPTS -o "Hg38ChrConverter.o" "../src/Hg38ChrConverter.cpp" +$CPP $CPP_OPTS -o "HelperFunctions.o" "../src/HelperFunctions.cpp" +$CPP $CPP_OPTS -o "sophiaMref.o" "../src/sophiaMref.cpp" +$CPP $CPP_OPTS -o "SuppAlignment.o" "../src/SuppAlignment.cpp" +$CPP $CPP_OPTS -o "SuppAlignmentAnno.o" "../src/SuppAlignmentAnno.cpp" +$CPP $CPP_OPTS -o "MrefEntry.o" "../src/MrefEntry.cpp" +$CPP $CPP_OPTS -o "MrefEntryAnno.o" "../src/MrefEntryAnno.cpp" +$CPP $CPP_OPTS -o "MrefMatch.o" "../src/MrefMatch.cpp" +$CPP $CPP_OPTS -o "MasterRefProcessor.o" "../src/MasterRefProcessor.cpp" +$CPP $CPP_OPTS -o "Breakpoint.o" "../src/Breakpoint.cpp" +$CPP $CPP_OPTS -o "BreakpointReduced.o" "../src/BreakpointReduced.cpp" +$CPP $CPP_OPTS -o "GermlineMatch.o" "../src/GermlineMatch.cpp" +$CPP $CPP_OPTS -o "DeFuzzier.o" "../src/DeFuzzier.cpp" + +$CPP -L$CONDA_PREFIX/lib -flto -o "sophiaMref" \ + GlobalAppConfig.o \ + ChrConverter.o \ + Hg37ChrConverter.o \ + Hg38ChrConverter.o \ + HelperFunctions.o \ + SuppAlignment.o \ + SuppAlignmentAnno.o \ + MrefEntry.o \ + MrefEntryAnno.o \ + MrefMatch.o \ + MasterRefProcessor.o \ + Breakpoint.o \ + BreakpointReduced.o \ + GermlineMatch.o \ + DeFuzzier.o \ + sophiaMref.o \ + -lz -lboost_system -lboost_iostreams -lboost_program_options From 769cce81612fe52c61c2b80e4cbf862b0710d839 Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Wed, 13 Dec 2023 11:50:06 +0100 Subject: [PATCH 03/79] Made README more descriptive with texts from Umut's and the SophiaWorkflow readmes. Signed-off-by: Philip R. Kensche --- README.md | 25 +++++++++++++++++++++++++ 1 file changed, 25 insertions(+) diff --git a/README.md b/README.md index 32d80be..1facc0b 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,30 @@ # SOPHIA Tool for Structural Variation Calling +SOPHIA is a Structural Variant(SV) detection algorithm based on the supplementary alignment(SA) concept of the aligner BWA-mem, combined with filters based on expert-knowledge to increase specificity. + +Currently, SOPHIA only is optimized for the hg38 assembly of the human genome. +It uses a large panel of normals for filtering artifacts (most often due to mapping difficulties) and common SVs in the germline. +The parameters for filtering results are hand-tuned against the clinical gold standard FISH of V(D)J rearrangements. +Results from the hand-tuned parameter set were tested against hallmark findings from disease datasets where hallmark SVs were known (CDKN2A in various TCGA datasets, EGFR in TCGA-GBM, GFI1B, MYCN and PRDM6 in ICGC-PEDBRAIN-MB etc.) + +For a detailed description of the algorithm, please refer to Umut Topraks's dissertation at https://doi.org/10.11588/heidok.00027429, in particular chapter 2. Section 2.2.1 describes the method in more details. + +SOPHIA is a very fast and resource-light algorithm. It uses 2GB RAM, 2 CPU cores and runs in ~3.5 hours for 50x coverage WGS, and can detect variants with a single pass of the input BAM files. No local assembly is done. + +> This is a fork of the original [SOPHIA](https://bitbucket.org/utoprak/sophia/src/master/) bitbucket repository. + +Sophia is included in the [SophiaWorkflow](https://github.com/DKFZ-ODCF/SophiaWorkflow) that uses the [Roddy Workflow Management Systems](https://github.com/TheRoddyWMS/Roddy). + + +### Citing + +You can cite Sophia as follows: + + Integrative Analysis of Omics Datasets. + Doctoral dissertation, German Cancer Research Center (DKFZ), Heidelberg. + Umut Toprak (2019). + DOI 10.11588/heidok.000274296 + ## Runtime Dependencies The only dependency is Boost 1.70.0 (currently). E.g. you can do From 0a9e8f229ec40e6e921444d23ed13ddf3e99fbce Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Wed, 13 Dec 2023 12:16:16 +0100 Subject: [PATCH 04/79] More code layout changes. Removed temporary file. Signed-off-by: Philip R. Kensche --- README.md | 2 +- src/AnnotationProcessor.cpp | 21 ++- src/BreakpointReduced.cpp | 29 ++-- src/DeFuzzier.cpp | 54 +++++-- src/IndexTable.cpp | 308 ------------------------------------ src/MrefEntry.cpp | 48 ++++-- src/SamSegmentMapper.cpp | 11 +- src/SuppAlignment.cpp | 13 +- src/sophia.cpp | 8 +- src/sophiaAnnotate.cpp | 23 ++- src/sophiaMref.cpp | 6 +- 11 files changed, 161 insertions(+), 362 deletions(-) delete mode 100644 src/IndexTable.cpp diff --git a/README.md b/README.md index 1facc0b..61ec2ce 100644 --- a/README.md +++ b/README.md @@ -90,4 +90,4 @@ STATIC=true build-sophiaAnnotate.sh * Patch: Use `namespace::std` to get rid of `std::` noise in the code * 9e3b6ed - * Last version in [bitbucket]](https://bitbucket.org/compbio_charite/sophia/src/master/) \ No newline at end of file + * Last version in [bitbucket](https://bitbucket.org/compbio_charite/sophia/src/master/) \ No newline at end of file diff --git a/src/AnnotationProcessor.cpp b/src/AnnotationProcessor.cpp index d67f78b..0a80b08 100644 --- a/src/AnnotationProcessor.cpp +++ b/src/AnnotationProcessor.cpp @@ -46,10 +46,14 @@ AnnotationProcessor::AnnotationProcessor(const string &tumorResultsIn, int defaultReadLengthTumorIn, bool controlCheckMode, int germlineDbLimit) - : NOCONTROLMODE{true}, GERMLINEDBLIMIT{germlineDbLimit}, - contaminationObserved{false}, massiveInvFilteringLevel{0}, - filteredResults{}, tumorResults{85, vector{}}, - controlResults{85, vector{}}, visitedLineIndices{} { + : NOCONTROLMODE{true}, + GERMLINEDBLIMIT{germlineDbLimit}, + contaminationObserved{false}, + massiveInvFilteringLevel{0}, + filteredResults{}, + tumorResults{85, vector{}}, + controlResults{85, vector{}}, + visitedLineIndices{} { unique_ptr tumorInputHandle{ make_unique(tumorResultsIn, ios_base::in | ios_base::binary)}; unique_ptr tumorGzHandle{ @@ -111,9 +115,12 @@ AnnotationProcessor::AnnotationProcessor( const string &controlResultsIn, int defaultReadLengthTumorIn, int defaultReadLengthControlIn, int germlineDbLimit, int lowQualControlIn, bool pathogenInControlIn) - : NOCONTROLMODE{false}, GERMLINEDBLIMIT{germlineDbLimit}, - contaminationObserved{false}, massiveInvFilteringLevel{0}, - filteredResults{}, tumorResults{85, vector{}}, + : NOCONTROLMODE{false}, + GERMLINEDBLIMIT{germlineDbLimit}, + contaminationObserved{false}, + massiveInvFilteringLevel{0}, + filteredResults{}, + tumorResults{85, vector{}}, controlResults{85, vector{}} { unique_ptr controlInputHandle{make_unique( controlResultsIn, ios_base::in | ios_base::binary)}; diff --git a/src/BreakpointReduced.cpp b/src/BreakpointReduced.cpp index 1a887ab..b1cfe84 100644 --- a/src/BreakpointReduced.cpp +++ b/src/BreakpointReduced.cpp @@ -107,15 +107,26 @@ BreakpointReduced::complexRearrangementMateRatioRescue(bool encounteredM) { sophia::BreakpointReduced::BreakpointReduced( const SuppAlignmentAnno &sa, const BreakpointReduced &emittingBp, bool fuzzySecondary) - : hasOverhang{false}, toRemove{false}, lineIndex{-1}, - chrIndex{sa.getChrIndex()}, pos{!fuzzySecondary ? sa.getPos() - : sa.getExtendedPos()}, - normalSpans{}, lowQualSpansSoft{}, lowQualSpansHard{}, - unpairedBreaksSoft{}, unpairedBreaksHard{}, breaksShortIndel{}, - lowQualBreaksSoft{}, lowQualBreaksHard{}, repetitiveOverhangBreaks{}, - pairedBreaksSoft{}, pairedBreaksHard{}, mateSupport{}, leftCoverage{}, - rightCoverage{}, mrefHits{MrefMatch{-1, -1, 10000, {}}}, - germlineInfo{GermlineMatch{0.0, 0.0, {}}}, suppAlignments{} { + : hasOverhang{false}, + toRemove{false}, lineIndex{-1}, + chrIndex{sa.getChrIndex()}, pos{!fuzzySecondary ? sa.getPos() : sa.getExtendedPos()}, + normalSpans{}, + lowQualSpansSoft{}, + lowQualSpansHard{}, + unpairedBreaksSoft{}, + unpairedBreaksHard{}, + breaksShortIndel{}, + lowQualBreaksSoft{}, + lowQualBreaksHard{}, + repetitiveOverhangBreaks{}, + pairedBreaksSoft{}, + pairedBreaksHard{}, + mateSupport{}, + leftCoverage{}, + rightCoverage{}, + mrefHits{MrefMatch{-1, -1, 10000, {}}}, + germlineInfo{GermlineMatch{0.0, 0.0, {}}}, + suppAlignments{} { addDummySa(sa, emittingBp); } diff --git a/src/DeFuzzier.cpp b/src/DeFuzzier.cpp index 42f833c..dff1fad 100644 --- a/src/DeFuzzier.cpp +++ b/src/DeFuzzier.cpp @@ -47,7 +47,9 @@ void DeFuzzier::deFuzzyDb(vector& bps) const { } } -void DeFuzzier::processFuzzySa(vector& bps, vector::iterator startingIt, SuppAlignmentAnno* startingSa) const { +void DeFuzzier::processFuzzySa(vector& bps, + vector::iterator startingIt, + SuppAlignmentAnno* startingSa) const { auto consensusSa = startingSa; vector processedSas { startingSa }; if (!startingSa->isEncounteredM()) { @@ -60,7 +62,11 @@ void DeFuzzier::processFuzzySa(vector& bps, vector& bps, vector::iterator startingIt, int increment, SuppAlignmentAnno* consensusSa, vector& processedSas) const { +void DeFuzzier::dbSweep(vector& bps, + vector::iterator startingIt, + int increment, + SuppAlignmentAnno* consensusSa, + vector& processedSas) const { auto it = startingIt; if (it == bps.begin() || it == bps.end()) { return; @@ -74,7 +80,10 @@ void DeFuzzier::dbSweep(vector& bps, vectorisFuzzy()) { - consensusSa->extendSuppAlignment(min(res->getPos(), consensusSa->getPos()), max(res->getExtendedPos(), consensusSa->getExtendedPos())); + consensusSa->extendSuppAlignment(min(res->getPos(), + consensusSa->getPos()), + max(res->getExtendedPos(), + consensusSa->getExtendedPos())); } } } @@ -82,7 +91,8 @@ void DeFuzzier::dbSweep(vector& bps, vector& processedSas, SuppAlignmentAnno* consensusSa) const { +void DeFuzzier::selectBestSa(vector& processedSas, + SuppAlignmentAnno* consensusSa) const { auto maxMateScore = -1; auto maxExpectedDiscordants = -1; auto index = 0; @@ -103,11 +113,15 @@ void DeFuzzier::selectBestSa(vector& processedSas, SuppAlign SuppAlignmentAnno* selectedSa = nullptr; if (!nonFuzzyIndices.empty()) { auto bestElement = max_element(nonFuzzyIndices.begin(), nonFuzzyIndices.end(), // - [&](int a, int b) {return processedSas[a]->getSupport()+processedSas[a]->getSecondarySupport() < processedSas[b]->getSupport()+processedSas[b]->getSecondarySupport();}); + [&](int a, int b) { + return processedSas[a]->getSupport()+processedSas[a]->getSecondarySupport() < processedSas[b]->getSupport()+processedSas[b]->getSecondarySupport(); + }); selectedSa = processedSas[*bestElement]; } else { auto bestElement = max_element(processedSas.begin(), processedSas.end(), // - [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) {return a->getMateSupport() < b->getMateSupport();}); + [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) { + return a->getMateSupport() < b->getMateSupport(); + }); selectedSa = *bestElement; selectedSa->extendSuppAlignment(consensusSa->getPos(), consensusSa->getExtendedPos()); } @@ -142,7 +156,9 @@ void DeFuzzier::deFuzzyDb(vector& bps) const { } } -void DeFuzzier::processFuzzySa(vector& bps, vector::iterator startingIt, SuppAlignmentAnno* startingSa) const { +void DeFuzzier::processFuzzySa(vector& bps, + vector::iterator startingIt, + SuppAlignmentAnno* startingSa) const { auto consensusSa = startingSa; unordered_set fileIndices { }; for (auto fileIndex : startingIt->getFileIndices()) { @@ -159,7 +175,12 @@ void DeFuzzier::processFuzzySa(vector& bps, vector::iterat selectBestSa(processedSas, consensusSa, fileIndices); } -void DeFuzzier::dbSweep(vector& bps, vector::iterator startingIt, unordered_set& fileIndices, int increment, SuppAlignmentAnno* consensusSa, vector& processedSas) const { +void DeFuzzier::dbSweep(vector& bps, + vector::iterator startingIt, + unordered_set& fileIndices, + int increment, + SuppAlignmentAnno* consensusSa, + vector& processedSas) const { auto it = startingIt; if (it == bps.begin() || it == bps.end()) { return; @@ -177,7 +198,10 @@ void DeFuzzier::dbSweep(vector& bps, vector::iterator star } processedSas.push_back(res); if (res->isFuzzy()) { - consensusSa->extendSuppAlignment(min(res->getPos(), consensusSa->getPos()), max(res->getExtendedPos(), consensusSa->getExtendedPos())); + consensusSa->extendSuppAlignment(min(res->getPos(), + consensusSa->getPos()), + max(res->getExtendedPos(), + consensusSa->getExtendedPos())); } } } @@ -186,7 +210,9 @@ void DeFuzzier::dbSweep(vector& bps, vector::iterator star } } -void DeFuzzier::selectBestSa(vector& processedSas, SuppAlignmentAnno* consensusSa, const unordered_set& fileIndices) const { +void DeFuzzier::selectBestSa(vector& processedSas, + SuppAlignmentAnno* consensusSa, + const unordered_set& fileIndices) const { auto maxMateScore = -1; auto maxExpectedDiscordants = -1; auto index = 0; @@ -207,11 +233,15 @@ void DeFuzzier::selectBestSa(vector& processedSas, SuppAlign SuppAlignmentAnno* selectedSa = nullptr; if (!nonFuzzyIndices.empty()) { auto bestElement = max_element(nonFuzzyIndices.begin(), nonFuzzyIndices.end(), // - [&](int a, int b) {return processedSas[a]->getSupportingIndices().size() < processedSas[b]->getSupportingIndices().size();}); + [&](int a, int b) { + return processedSas[a]->getSupportingIndices().size() < processedSas[b]->getSupportingIndices().size(); + }); selectedSa = processedSas[*bestElement]; } else { auto bestElement = max_element(processedSas.begin(), processedSas.end(), // - [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) {return a->getSupportingIndices().size() < b->getSupportingIndices().size();}); + [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) { + return a->getSupportingIndices().size() < b->getSupportingIndices().size(); + }); if ((*bestElement)->getSupport() + (*bestElement)->getSecondarySupport() == 0) { if (consensusSa->isEncounteredM()) { selectedSa = processedSas.back(); diff --git a/src/IndexTable.cpp b/src/IndexTable.cpp deleted file mode 100644 index 50a7f06..0000000 --- a/src/IndexTable.cpp +++ /dev/null @@ -1,308 +0,0 @@ -/* - * Hg37IndexTable.cpp - * - * Created on: 28 Dec 2017 - * Author: Umut H. Toprak, DKFZ Heidelberg (Divisions of Theoretical - * Bioinformatics, Bioinformatics and Omics Data Analytics and currently - * Neuroblastoma Genomics) Copyright (C) 2018 Umut H. Toprak, Matthias - * Schlesner, Roland Eils and DKFZ Heidelberg - * - * This program is free software: you can redistribute it and/or modify - * it under the terms of the GNU General Public License as published by - * the Free Software Foundation, either version 3 of the License, or - * (at your option) any later version. - * - * This program is distributed in the hope that it will be useful, - * but WITHOUT ANY WARRANTY; without even the implied warranty of - * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the - * GNU General Public License for more details. - * - * You should have received a copy of the GNU General Public License - * along with this program. If not, see . - * LICENSE: GPL - */ - -#include - -namespace sophia { - - using namespace std; - - const array Hg37IndexTable::indexToChr { - "0", "1", "2", "3", "4", - "5", "6", "7", "8", "9", - "10", "11", "12", "13", "14", - "15", "16", "17", "18", "19", - "20", "21", "22", "23", "24", - "25", "26", "27", "28", "29", - "30", "31", "32", "33", "34", - "35", "36", "37", "38", "39", - "X", "Y", "42", "43", "44", - "45", "46", "47", "48", "49", - "50", "51", "52", "53", "54", - "55", "56", "57", "58", "59", - "60", "61", "62", "63", "64", - "65", "66", "67", "68", "69", - "70", "71", "72", "73", "74", - "75", "76", "77", "78", "79", - "80", "81", "82", "83", "84", - "85", "86", "87", "88", "89", - "90", "91", "92", "93", "94", - "95", "96", "97", "98", "99", - "100", "101", "102", "103", "104", - "105", "106", "107", "108", "109", - "110", "111", "112", "113", "114", - "115", "116", "117", "118", "119", - "120", "121", "122", "123", "124", - "125", "126", "127", "128", "129", - "130", "131", "132", "133", "134", - "135", "136", "137", "138", "139", - "140", "141", "142", "143", "144", - "145", "146", "147", "148", "149", - "150", "151", "152", "153", "154", - "155", "156", "157", "158", "159", - "160", "161", "162", "163", "164", - "165", "166", "167", "168", "169", - "170", "171", "172", "173", "174", - "175", "176", "177", "178", "179", - "180", "181", "182", "183", "184", - "185", "186", "187", "188", "189", - "190", "GL000191.1", "GL000192.1", "GL000193.1", "GL000194.1", - "GL000195.1", "GL000196.1", "GL000197.1", "GL000198.1", "GL000199.1", - "GL000200.1", "GL000201.1", "GL000202.1", "GL000203.1", "GL000204.1", - "GL000205.1", "GL000206.1", "GL000207.1", "GL000208.1", "GL000209.1", - "GL000210.1", "GL000211.1", "GL000212.1", "GL000213.1", "GL000214.1", - "GL000215.1", "GL000216.1", "GL000217.1", "GL000218.1", "GL000219.1", - "GL000220.1", "GL000221.1", "GL000222.1", "GL000223.1", "GL000224.1", - "GL000225.1", "GL000226.1", "GL000227.1", "GL000228.1", "GL000229.1", - "GL000230.1", "GL000231.1", "GL000232.1", "GL000233.1", "GL000234.1", - "GL000235.1", "GL000236.1", "GL000237.1", "GL000238.1", "GL000239.1", - "GL000240.1", "GL000241.1", "GL000242.1", "GL000243.1", "GL000244.1", - "GL000245.1", "GL000246.1", "GL000247.1", "GL000248.1", "GL000249.1", - "250", "251", "252", "253", "254", - "255", "256", "257", "258", "259", - "260", "261", "262", "263", "264", - "265", "266", "267", "268", "269", - "270", "271", "272", "273", "274", - "275", "276", "277", "278", "279", - "280", "281", "282", "283", "284", - "285", "286", "287", "288", "289", - "290", "291", "292", "293", "294", - "295", "296", "297", "298", "299", - "300", "301", "302", "303", "304", - "305", "306", "307", "308", "309", - "310", "311", "312", "313", "314", - "315", "316", "317", "318", "319", - "320", "321", "322", "323", "324", - "325", "326", "327", "328", "329", - "330", "331", "332", "333", "334", - "335", "336", "337", "338", "339", - "340", "341", "342", "343", "344", - "345", "346", "347", "348", "349", - "350", "351", "352", "353", "354", - "355", "356", "357", "358", "359", - "360", "361", "362", "363", "364", - "365", "366", "367", "368", "369", - "370", "371", "372", "373", "374", - "375", "376", "377", "378", "379", - "380", "381", "382", "383", "384", - "385", "386", "387", "388", "389", - "390", "391", "392", "393", "394", - "395", "396", "397", "398", "399", - "400", "401", "402", "403", "404", - "405", "406", "407", "408", "409", - "410", "411", "412", "413", "414", - "415", "416", "417", "418", "419", - "420", "421", "422", "423", "424", - "425", "426", "427", "428", "429", - "430", "431", "432", "433", "434", - "435", "436", "437", "438", "439", - "440", "441", "442", "443", "444", - "445", "446", "447", "448", "449", - "450", "451", "452", "453", "454", - "455", "456", "457", "458", "459", - "460", "461", "462", "463", "464", - "465", "466", "467", "468", "469", - "470", "471", "472", "473", "474", - "475", "476", "477", "478", "479", - "480", "481", "482", "483", "484", - "485", "486", "487", "488", "489", - "490", "491", "492", "493", "494", - "495", "496", "497", "498", "499", - "500", "501", "502", "503", "504", - "505", "506", "507", "508", "509", - "510", "511", "512", "513", "514", - "515", "516", "517", "518", "519", - "520", "521", "522", "523", "524", - "525", "526", "527", "528", "529", - "530", "531", "532", "533", "534", - "535", "536", "537", "538", "539", - "540", "541", "542", "543", "544", - "545", "546", "547", "548", "549", - "550", "551", "552", "553", "554", - "555", "556", "557", "558", "559", - "560", "561", "562", "563", "564", - "565", "566", "567", "568", "569", - "570", "571", "572", "573", "574", - "575", "576", "577", "578", "579", - "580", "581", "582", "583", "584", - "585", "586", "587", "588", "589", - "590", "591", "592", "593", "594", - "595", "596", "597", "598", "599", - "600", "601", "602", "603", "604", - "605", "606", "607", "608", "609", - "610", "611", "612", "613", "614", - "615", "616", "617", "618", "619", - "620", "621", "622", "623", "624", - "625", "626", "627", "628", "629", - "630", "631", "632", "633", "634", - "635", "636", "637", "638", "639", - "640", "641", "642", "643", "644", - "645", "646", "647", "648", "649", - "650", "651", "652", "653", "654", - "655", "656", "657", "658", "659", - "660", "661", "662", "663", "664", - "665", "666", "667", "668", "669", - "670", "671", "672", "673", "674", - "675", "676", "677", "678", "679", - "680", "681", "682", "683", "684", - "685", "686", "687", "688", "689", - "690", "691", "692", "693", "694", - "695", "696", "697", "698", "699", - "700", "701", "702", "703", "704", - "705", "706", "707", "708", "709", - "710", "711", "712", "713", "714", - "715", "716", "717", "718", "719", - "720", "721", "722", "723", "724", - "725", "726", "727", "728", "729", - "730", "731", "732", "733", "734", - "735", "736", "737", "738", "739", - "740", "741", "742", "743", "744", - "745", "746", "747", "748", "749", - "750", "751", "752", "753", "754", - "755", "756", "757", "758", "759", - "760", "761", "762", "763", "764", - "765", "766", "767", "768", "769", - "770", "771", "772", "773", "774", - "775", "776", "777", "778", "779", - "780", "781", "782", "783", "784", - "785", "786", "787", "788", "789", - "790", "791", "792", "793", "794", - "795", "796", "797", "798", "799", - "800", "801", "802", "803", "804", - "805", "806", "807", "808", "809", - "810", "811", "812", "813", "814", - "815", "816", "817", "818", "819", - "820", "821", "822", "823", "824", - "825", "826", "827", "828", "829", - "830", "831", "832", "833", "834", - "835", "836", "837", "838", "839", - "840", "841", "842", "843", "844", - "845", "846", "847", "848", "849", - "850", "851", "852", "853", "854", - "855", "856", "857", "858", "859", - "860", "861", "862", "863", "864", - "865", "866", "867", "868", "869", - "870", "871", "872", "873", "874", - "875", "876", "877", "878", "879", - "880", "881", "882", "883", "884", - "885", "886", "887", "888", "889", - "890", "891", "892", "893", "894", - "895", "896", "897", "898", "899", - "900", "901", "902", "903", "904", - "905", "906", "907", "908", "909", - "910", "911", "912", "913", "914", - "915", "916", "917", "918", "919", - "920", "921", "922", "923", "924", - "925", "926", "927", "928", "929", - "930", "931", "932", "933", "934", - "935", "936", "937", "938", "939", - "940", "941", "942", "943", "944", - "945", "946", "947", "948", "949", - "950", "951", "952", "953", "954", - "955", "956", "957", "958", "959", - "960", "961", "962", "963", "964", - "965", "966", "967", "968", "969", - "970", "971", "972", "973", "974", - "975", "976", "977", "978", "979", - "980", "981", "982", "983", "984", - "985", "986", "987", "988", "989", - "990", "991", "992", "993", "994", - "995", "996", "997", "998", "hs37d5", - "NC_007605", "MT", "phiX174", "INVALID"}; - - const array Hg37IndexTable::indexToChrCompressedMref { - "1", "2", "3", "4", "5", - "6", "7", "8", "9", "10", - "11", "12", "13", "14", "15", - "16", "17", "18", "19", "20", - "21", "22", "X", "Y", "GL000191.1", - "GL000192.1", "GL000193.1", "GL000194.1", "GL000195.1", "GL000196.1", - "GL000197.1", "GL000198.1", "GL000199.1", "GL000200.1", "GL000201.1", - "GL000202.1", "GL000203.1", "GL000204.1", "GL000205.1", "GL000206.1", - "GL000207.1", "GL000208.1", "GL000209.1", "GL000210.1", "GL000211.1", - "GL000212.1", "GL000213.1", "GL000214.1", "GL000215.1", "GL000216.1", - "GL000217.1", "GL000218.1", "GL000219.1", "GL000220.1", "GL000221.1", - "GL000222.1", "GL000223.1", "GL000224.1", "GL000225.1", "GL000226.1", - "GL000227.1", "GL000228.1", "GL000229.1", "GL000230.1", "GL000231.1", - "GL000232.1", "GL000233.1", "GL000234.1", "GL000235.1", "GL000236.1", - "GL000237.1", "GL000238.1", "GL000239.1", "GL000240.1", "GL000241.1", - "GL000242.1", "GL000243.1", "GL000244.1", "GL000245.1", "GL000246.1", - "GL000247.1", "GL000248.1", "GL000249.1", "hs37d5", "NC_007605"}; - - const array Hg37IndexTable::indexConverter { - -2, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, - 18, 19, 20, 21, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, 22, 23, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, - 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, - 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, - 80, 81, 82, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, 83, 84, -2, -2, -2}; - -} /* namespace sophia */ diff --git a/src/MrefEntry.cpp b/src/MrefEntry.cpp index 11405f1..dc4823f 100644 --- a/src/MrefEntry.cpp +++ b/src/MrefEntry.cpp @@ -49,8 +49,16 @@ MrefEntry::MrefEntry() : void MrefEntry::addEntry(BreakpointReduced& tmpBreakpoint, int fileIndex) { pos = tmpBreakpoint.getPos(); - auto artifactBreakTotal = tmpBreakpoint.getLowQualBreaksSoft() + tmpBreakpoint.getLowQualBreaksHard() + tmpBreakpoint.getRepetitiveOverhangBreaks(); - auto eventTotal = tmpBreakpoint.getPairedBreaksSoft() + tmpBreakpoint.getPairedBreaksHard() + tmpBreakpoint.getUnpairedBreaksSoft() + tmpBreakpoint.getUnpairedBreaksHard() + tmpBreakpoint.getBreaksShortIndel(); + auto artifactBreakTotal = + tmpBreakpoint.getLowQualBreaksSoft() + + tmpBreakpoint.getLowQualBreaksHard() + + tmpBreakpoint.getRepetitiveOverhangBreaks(); + auto eventTotal = + tmpBreakpoint.getPairedBreaksSoft() + + tmpBreakpoint.getPairedBreaksHard() + + tmpBreakpoint.getUnpairedBreaksSoft() + + tmpBreakpoint.getUnpairedBreaksHard() + + tmpBreakpoint.getBreaksShortIndel(); auto breakTotal = eventTotal + artifactBreakTotal; if (breakTotal < 200) { const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); @@ -64,7 +72,8 @@ void MrefEntry::addEntry(BreakpointReduced& tmpBreakpoint, int fileIndex) { auto qualCheck = false; auto splitTotal = saPtr->getSupport() + saPtr->getSecondarySupport(); if (saPtr->isDistant()) { - auto clonalityCondition = (((0.0 + saPtr->getMateSupport()) / saPtr->getExpectedDiscordants()) >= 0.5); + auto clonalityCondition = + (((0.0 + saPtr->getMateSupport()) / saPtr->getExpectedDiscordants()) >= 0.5); if (!clonalityCondition) { continue; } @@ -73,7 +82,9 @@ void MrefEntry::addEntry(BreakpointReduced& tmpBreakpoint, int fileIndex) { qualCheck = (saPtr->getMateSupport() > 4); } } else { - qualCheck = (splitTotal > 4) || (splitTotal > 2 && saPtr->getSupport() > 0 && saPtr->getSecondarySupport() > 0); + qualCheck = (splitTotal > 4) + || (splitTotal > 2 && saPtr->getSupport() > 0 + && saPtr->getSecondarySupport() > 0); } if (qualCheck) { if (!saMatcher(saPtr)) { @@ -99,10 +110,17 @@ void MrefEntry::addEntry(BreakpointReduced& tmpBreakpoint, int fileIndex) { } if (eventTotal + artifactBreakTotal > 0) { if (covValidity) { - auto eventTotalStrict = tmpBreakpoint.getPairedBreaksSoft() + tmpBreakpoint.getUnpairedBreaksSoft() + tmpBreakpoint.getPairedBreaksHard(); - auto artifactTotalRelaxed = tmpBreakpoint.getLowQualBreaksSoft() + tmpBreakpoint.getLowQualSpansSoft() + tmpBreakpoint.getRepetitiveOverhangBreaks(); + auto eventTotalStrict = + tmpBreakpoint.getPairedBreaksSoft() + + tmpBreakpoint.getUnpairedBreaksSoft() + + tmpBreakpoint.getPairedBreaksHard(); + auto artifactTotalRelaxed = + tmpBreakpoint.getLowQualBreaksSoft() + + tmpBreakpoint.getLowQualSpansSoft() + + tmpBreakpoint.getRepetitiveOverhangBreaks(); if ((eventTotalStrict + artifactTotalRelaxed) > 0) { - artifactRatios.push_back((0.0 + artifactTotalRelaxed) / (eventTotalStrict + artifactTotalRelaxed)); + artifactRatios.push_back( + (0.0 + artifactTotalRelaxed) / (eventTotalStrict + artifactTotalRelaxed)); fileIndicesWithArtifactRatios.push_back(fileIndex); } } @@ -146,7 +164,8 @@ string MrefEntry::printBpInfo(const string& chromosome) { outputFields.emplace_back(boost::str(doubleFormatter % ((fileIndices.size() + 0.0) / NUMPIDS))); outputFields.emplace_back(boost::str(doubleFormatter % ((fileIndicesWithArtifactRatios.size() + 0.0) / NUMPIDS))); if (!artifactRatios.empty()) { - outputFields.emplace_back(boost::str(doubleFormatter % (accumulate(artifactRatios.cbegin(), artifactRatios.cend(), 0.0) / artifactRatios.size()))); + outputFields.emplace_back(boost::str(doubleFormatter % (accumulate(artifactRatios.cbegin(), + artifactRatios.cend(), 0.0) / artifactRatios.size()))); } else { outputFields.emplace_back("NA"); } @@ -168,7 +187,10 @@ string MrefEntry::printBpInfo(const string& chromosome) { } } vector fileIndicesStr { }; - transform(fileIndices.begin(), fileIndices.end(), back_inserter(fileIndicesStr), [](int fileIndex) {return strtk::type_to_string(fileIndex);}); + transform(fileIndices.begin(), + fileIndices.end(), + back_inserter(fileIndicesStr), + [](int fileIndex) {return strtk::type_to_string(fileIndex);}); outputFields.emplace_back(boost::join(fileIndicesStr, ",")); return boost::join(outputFields, "\t").append("\n"); } @@ -181,7 +203,8 @@ string MrefEntry::printArtifactRatios(const string& chromosome) { outputFields.emplace_back(strtk::type_to_string(pos + 1)); vector artifactRatiosOutput(NUMPIDS, "."); for (auto i = 0; i < static_cast(fileIndicesWithArtifactRatios.size()); ++i) { - artifactRatiosOutput[fileIndicesWithArtifactRatios[i]] = boost::str(doubleFormatter % artifactRatios[i]); + artifactRatiosOutput[fileIndicesWithArtifactRatios[i]] = + boost::str(doubleFormatter % artifactRatios[i]); } for (const auto &artifactRatio : artifactRatiosOutput) { outputFields.push_back(artifactRatio); @@ -204,7 +227,10 @@ SuppAlignmentAnno* MrefEntry::searchFuzzySa(const SuppAlignmentAnno& fuzzySa) { } bool MrefEntry::saMatcher(SuppAlignmentAnno* saPtr) { - if (saPtr->isToRemove() || saPtr->isSuspicious() || (saPtr->getExpectedDiscordants() > 0 && !(saPtr->getMateSupport() / (saPtr->getExpectedDiscordants() + 0.0) > 0.1))) { + if (saPtr->isToRemove() + || saPtr->isSuspicious() + || (saPtr->getExpectedDiscordants() > 0 + && !(saPtr->getMateSupport() / (saPtr->getExpectedDiscordants() + 0.0) > 0.1))) { return true; } for (auto &sa : suppAlignments) { diff --git a/src/SamSegmentMapper.cpp b/src/SamSegmentMapper.cpp index 2ed4346..38be542 100644 --- a/src/SamSegmentMapper.cpp +++ b/src/SamSegmentMapper.cpp @@ -37,9 +37,14 @@ SamSegmentMapper::SamSegmentMapper(int defaultReadLengthIn) PROPERPARIRCOMPENSATIONMODE{Breakpoint::PROPERPAIRCOMPENSATIONMODE}, DISCORDANTLEFTRANGE{static_cast(round(defaultReadLengthIn * 3))}, DISCORDANTRIGHTRANGE{static_cast(round(defaultReadLengthIn * 2.51))}, - printedBps{0u}, chrIndexCurrent{0}, minPos{-1}, maxPos{-1}, - breakpointsCurrent{}, discordantAlignmentsPool{}, - discordantAlignmentCandidatesPool{}, discordantLowQualAlignmentsPool{} {} + printedBps{0u}, + chrIndexCurrent{0}, + minPos{-1}, + maxPos{-1}, + breakpointsCurrent{}, + discordantAlignmentsPool{}, + discordantAlignmentCandidatesPool{}, + discordantLowQualAlignmentsPool{} {} void SamSegmentMapper::parseSamStream() { diff --git a/src/SuppAlignment.cpp b/src/SuppAlignment.cpp index 3fabcee..099d37a 100644 --- a/src/SuppAlignment.cpp +++ b/src/SuppAlignment.cpp @@ -104,7 +104,11 @@ SuppAlignment::SuppAlignment(string::const_iterator saCbegin, //cerr << "guessSupplementOffset" << endl; vector cigarChunks; auto cigarEncounteredM = false; - auto cumulativeNucleotideCount = 0, currentNucleotideCount = 0, chunkIndex = 0, bestChunkIndex = 0, indelAdjustment = 0; + auto cumulativeNucleotideCount = 0, + currentNucleotideCount = 0, + chunkIndex = 0, + bestChunkIndex = 0, + indelAdjustment = 0; auto largestClip = 0; auto leftClipAdjustment = 0; for (auto cigarString_cit = fieldBegins[3]; cigarString_cit != fieldEnds[3]; ++cigarString_cit) { @@ -178,7 +182,8 @@ SuppAlignment::SuppAlignment(string::const_iterator saCbegin, void SuppAlignment::finalizeSupportingIndices() { sort(supportingIndices.begin(), supportingIndices.end()); sort(supportingIndicesSecondary.begin(), supportingIndicesSecondary.end()); - supportingIndices.erase(unique(supportingIndices.begin(), supportingIndices.end()), supportingIndices.end()); + supportingIndices.erase(unique(supportingIndices.begin(), supportingIndices.end()), + supportingIndices.end()); supportingIndicesSecondary.erase(unique(supportingIndicesSecondary.begin(), supportingIndicesSecondary.end()), supportingIndicesSecondary.end()); @@ -367,7 +372,9 @@ SuppAlignment::SuppAlignment(const string& saIn) : } bool SuppAlignment::saCloseness(const SuppAlignment& rhs, int fuzziness) const { - if (inverted == rhs.isInverted() && chrIndex == rhs.getChrIndex() && encounteredM == rhs.isEncounteredM()) { + if (inverted == rhs.isInverted() + && chrIndex == rhs.getChrIndex() + && encounteredM == rhs.isEncounteredM()) { if (strictFuzzy || rhs.isStrictFuzzy()) { fuzziness = 2.5 * DEFAULTREADLENGTH; return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && diff --git a/src/sophia.cpp b/src/sophia.cpp index f8e4a17..0ecbb21 100644 --- a/src/sophia.cpp +++ b/src/sophia.cpp @@ -109,12 +109,16 @@ int main(int argc, char** argv) { if (inputVariables.count("medianisize") && inputVariables.count("stdisizepercentage")) { auto medianIsize = inputVariables["medianisize"].as(); auto isizeStdPercentage = inputVariables["stdisizepercentage"].as(); - Alignment::ISIZEMAX = min(4000.0, medianIsize + isizeSigmaLevel * medianIsize * isizeStdPercentage * 0.01); + Alignment::ISIZEMAX = + min(4000.0, + medianIsize + isizeSigmaLevel * medianIsize * isizeStdPercentage * 0.01); SuppAlignment::ISIZEMAX = Alignment::ISIZEMAX; } else { Alignment::ISIZEMAX = 2000.0; SuppAlignment::ISIZEMAX = 2000.0; - cerr << "No insert size distribution file given, using a dummy default value of 2000 as the min insert size of a distant event" << endl; + cerr << "No insert size distribution file given, using a dummy default value of 2000 " + << "as the min insert size of a distant event" + << endl; } } diff --git a/src/sophiaAnnotate.cpp b/src/sophiaAnnotate.cpp index 0c6ca29..194f7b9 100644 --- a/src/sophiaAnnotate.cpp +++ b/src/sophiaAnnotate.cpp @@ -143,8 +143,12 @@ int main(int argc, char** argv) { const GlobalAppConfig &config = GlobalAppConfig::init(move(chrConverter)); MrefEntryAnno::PIDSINMREF = pidsInMref; - unique_ptr mrefInputHandle { make_unique(options["mref"].as(), ios_base::in | ios_base::binary) }; - unique_ptr mrefGzHandle { make_unique() }; + unique_ptr mrefInputHandle { + make_unique(options["mref"].as(), ios_base::in | ios_base::binary) + }; + unique_ptr mrefGzHandle { + make_unique() + }; mrefGzHandle->push(boost::iostreams::gzip_decompressor()); mrefGzHandle->push(*mrefInputHandle); cerr << "m\n"; @@ -201,12 +205,23 @@ int main(int argc, char** argv) { auto pathogenInControl = false; { SvEvent::NOCONTROLMODE = true; - AnnotationProcessor annotationProcessorControlCheck { controlResults, mref, defaultReadLengthControl, true, germlineDbLimit }; + AnnotationProcessor annotationProcessorControlCheck { + controlResults, mref, defaultReadLengthControl, true, germlineDbLimit + }; lowQualControl = annotationProcessorControlCheck.getMassiveInvFilteringLevel(); pathogenInControl = annotationProcessorControlCheck.isContaminationObserved(); SvEvent::NOCONTROLMODE = false; } - AnnotationProcessor annotationProcessor { tumorResults, mref, controlResults, defaultReadLengthTumor, defaultReadLengthControl, germlineDbLimit, lowQualControl, pathogenInControl }; + AnnotationProcessor annotationProcessor { + tumorResults, + mref, + controlResults, + defaultReadLengthTumor, + defaultReadLengthControl, + germlineDbLimit, + lowQualControl, + pathogenInControl + }; annotationProcessor.printFilteredResults(pathogenInControl, lowQualControl); } else { SvEvent::NOCONTROLMODE = true; diff --git a/src/sophiaMref.cpp b/src/sophiaMref.cpp index 67dfce2..b31634a 100644 --- a/src/sophiaMref.cpp +++ b/src/sophiaMref.cpp @@ -38,7 +38,8 @@ int main(int argc, char** argv) { "outputrootname"); boost::program_options::variables_map inputVariables { }; - boost::program_options::store(boost::program_options::parse_command_line(argc, argv, desc), inputVariables); + boost::program_options::store(boost::program_options::parse_command_line(argc, argv, desc), + inputVariables); boost::program_options::notify(inputVariables); if (inputVariables.count("help")) { @@ -91,7 +92,8 @@ int main(int argc, char** argv) { } else if (inputVariables["assemblyname"].as() == Hg38ChrConverter::assembly_name) { chrConverter = unique_ptr(new Hg38ChrConverter()); } else { - cerr << "Unknown assembly name " << inputVariables["assemblyname"].as() << ". I know " + cerr << "Unknown assembly name " << inputVariables["assemblyname"].as() + << ". I know " << Hg37ChrConverter::assembly_name << " and " << Hg38ChrConverter::assembly_name << endl; return 1; From a5ebdb6b7ea0e382c275d986a380b62b360fd2b4 Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Wed, 13 Dec 2023 13:14:09 +0100 Subject: [PATCH 05/79] Removed using namespace std. More code layout changes. Signed-off-by: Philip R. Kensche --- src/Alignment.cpp | 1240 +++++++------- src/AnnotationProcessor.cpp | 1866 +++++++++++---------- src/Breakpoint.cpp | 2328 +++++++++++++------------- src/BreakpointReduced.cpp | 306 ++-- src/ChosenBp.cpp | 56 +- src/ChrConverter.cpp | 2 - src/DeFuzzier.cpp | 450 +++-- src/GermlineMatch.cpp | 20 +- src/GlobalAppConfig.cpp | 4 +- src/HelperFunctions.cpp | 2 - src/Hg37ChrConverter.cpp | 10 +- src/Hg38ChrConverter.cpp | 10 +- src/MasterRefProcessor.cpp | 242 ++- src/MrefEntry.cpp | 438 +++-- src/MrefEntryAnno.cpp | 123 +- src/MrefMatch.cpp | 10 +- src/SamSegmentMapper.cpp | 720 ++++---- src/Sdust.cpp | 190 ++- src/SuppAlignment.cpp | 720 ++++---- src/SuppAlignmentAnno.cpp | 467 +++--- src/SvEvent.cpp | 3106 ++++++++++++++++++----------------- src/sophia.cpp | 33 +- src/sophiaAnnotate.cpp | 1 - src/sophiaMref.cpp | 1 - 24 files changed, 6157 insertions(+), 6188 deletions(-) diff --git a/src/Alignment.cpp b/src/Alignment.cpp index 0bd32fc..26c9b37 100644 --- a/src/Alignment.cpp +++ b/src/Alignment.cpp @@ -34,733 +34,731 @@ namespace sophia { -using namespace std; + int Alignment::LOWQUALCLIPTHRESHOLD{}, + Alignment::BASEQUALITYTHRESHOLD{}, + Alignment::BASEQUALITYTHRESHOLDLOW{}, + Alignment::CLIPPEDNUCLEOTIDECOUNTTHRESHOLD{}, + Alignment::INDELNUCLEOTIDECOUNTTHRESHOLD{}; -int Alignment::LOWQUALCLIPTHRESHOLD{}, - Alignment::BASEQUALITYTHRESHOLD{}, - Alignment::BASEQUALITYTHRESHOLDLOW{}, - Alignment::CLIPPEDNUCLEOTIDECOUNTTHRESHOLD{}, - Alignment::INDELNUCLEOTIDECOUNTTHRESHOLD{}; - -double Alignment::ISIZEMAX{}; -Alignment::Alignment() - : lowMapq(false), - nullMapq(true), - distantMate(0), - chosenBp(nullptr), - chrIndex(0), - readType(0), - startPos(0), - endPos(0), - mateChrIndex(0), - matePos(0), - samLine(), - validLine(error_terminating_getline(cin, samLine)), - samChunkPositions(), - saCbegin(), - saCend(), - hasSa(false), - supplementary(false), - fwdStrand(true), - invertedMate(false), - qualChecked(false) { - if (validLine) { - auto index = 0; - for (auto it = samLine.cbegin(); it != samLine.cend(); ++it) { - if (*it == '\t') { - samChunkPositions.push_back(index); + double Alignment::ISIZEMAX{}; + Alignment::Alignment() + : lowMapq(false), + nullMapq(true), + distantMate(0), + chosenBp(nullptr), + chrIndex(0), + readType(0), + startPos(0), + endPos(0), + mateChrIndex(0), + matePos(0), + samLine(), + validLine(error_terminating_getline(cin, samLine)), + samChunkPositions(), + saCbegin(), + saCend(), + hasSa(false), + supplementary(false), + fwdStrand(true), + invertedMate(false), + qualChecked(false) { + if (validLine) { + auto index = 0; + for (auto it = samLine.cbegin(); it != samLine.cend(); ++it) { + if (*it == '\t') { + samChunkPositions.push_back(index); + } + ++index; } - ++index; + chrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex( + next(samLine.cbegin(), samChunkPositions[1] + 1), '\t'); } - chrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex( - next(samLine.cbegin(), samChunkPositions[1] + 1), '\t'); - } -} - -void -Alignment::continueConstruction() { - mappingQualityCheck(); - for (auto startPos_cit = samLine.cbegin() + 1 + samChunkPositions[2]; - startPos_cit != samLine.cbegin() + samChunkPositions[3]; - ++startPos_cit) { - startPos = startPos * 10 + (*startPos_cit - '0'); } - auto readLength = (samChunkPositions[9] - samChunkPositions[8] - 1); - endPos = startPos + readLength; - auto flag = 0; - for (auto flag_cit = samLine.cbegin() + 1 + samChunkPositions[0]; - flag_cit != samLine.cbegin() + samChunkPositions[1]; ++flag_cit) { - flag = flag * 10 + (*flag_cit - '0'); - } - auto flags = bitset<12>(flag); - supplementary = (flags[11] == true); - fwdStrand = (flags[4] == false); - auto mateFwdStrand = (flags[5] == false); - invertedMate = (fwdStrand == mateFwdStrand); + void + Alignment::continueConstruction() { + mappingQualityCheck(); + for (auto startPos_cit = samLine.cbegin() + 1 + samChunkPositions[2]; + startPos_cit != samLine.cbegin() + samChunkPositions[3]; + ++startPos_cit) { + startPos = startPos * 10 + (*startPos_cit - '0'); + } + auto readLength = (samChunkPositions[9] - samChunkPositions[8] - 1); + endPos = startPos + readLength; - bool eventCandidate = isEventCandidate(); - if (eventCandidate) { - createCigarChunks(); - assignBreakpointsAndOverhangs(); - if (supplementary) { - auto startCit = next(samLine.cbegin(), 1 + samChunkPositions[9]); - auto endCit = next(samLine.cbegin(), samChunkPositions[10]); - vector overhangPerBaseQuality{}; - fullMedianQuality(startCit, endCit, overhangPerBaseQuality); - if (overhangPerBaseQuality.empty() || - getMedian(overhangPerBaseQuality.begin(), - overhangPerBaseQuality.end()) < - BASEQUALITYTHRESHOLD) { - readType = 5; - } else { - readType = 2; - } + auto flag = 0; + for (auto flag_cit = samLine.cbegin() + 1 + samChunkPositions[0]; + flag_cit != samLine.cbegin() + samChunkPositions[1]; ++flag_cit) { + flag = flag * 10 + (*flag_cit - '0'); } - if (readType == 7) { + auto flags = bitset<12>(flag); + supplementary = (flags[11] == true); + fwdStrand = (flags[4] == false); + auto mateFwdStrand = (flags[5] == false); + invertedMate = (fwdStrand == mateFwdStrand); + + bool eventCandidate = isEventCandidate(); + if (eventCandidate) { + createCigarChunks(); + assignBreakpointsAndOverhangs(); if (supplementary) { - if (uniqueSuppCheck() && hasSa) { + auto startCit = next(samLine.cbegin(), 1 + samChunkPositions[9]); + auto endCit = next(samLine.cbegin(), samChunkPositions[10]); + vector overhangPerBaseQuality{}; + fullMedianQuality(startCit, endCit, overhangPerBaseQuality); + if (overhangPerBaseQuality.empty() || + getMedian(overhangPerBaseQuality.begin(), + overhangPerBaseQuality.end()) < + BASEQUALITYTHRESHOLD) { + readType = 5; + } else { readType = 2; + } + } + if (readType == 7) { + if (supplementary) { + if (uniqueSuppCheck() && hasSa) { + readType = 2; + } else { + readType = 5; + } } else { readType = 5; - } - } else { - readType = 5; - auto rescueCandidate = false; - for (const auto &cigarChunk : cigarChunks) { - if (cigarChunk.chunkType == 'S') { - auto medianQual = overhangMedianQuality(cigarChunk); - if (cigarChunk.length > LOWQUALCLIPTHRESHOLD && - medianQual < BASEQUALITYTHRESHOLD) { - rescueCandidate = false; - break; - } - if (cigarChunk.length / (readLength + 0.0) > 0.5) { - if (medianQual >= BASEQUALITYTHRESHOLD) { - rescueCandidate = true; + auto rescueCandidate = false; + for (const auto &cigarChunk : cigarChunks) { + if (cigarChunk.chunkType == 'S') { + auto medianQual = overhangMedianQuality(cigarChunk); + if (cigarChunk.length > LOWQUALCLIPTHRESHOLD && + medianQual < BASEQUALITYTHRESHOLD) { + rescueCandidate = false; + break; + } + if (cigarChunk.length / (readLength + 0.0) > 0.5) { + if (medianQual >= BASEQUALITYTHRESHOLD) { + rescueCandidate = true; + } } } } + if (rescueCandidate) { + readType = 1; + } + qualChecked = true; } - if (rescueCandidate) { - readType = 1; - } - qualChecked = true; } + if (readType < 5) { + qualityCheckCascade(); + } + } else if (readType == 7) { + readType = 5; } - if (readType < 5) { - qualityCheckCascade(); - } - } else if (readType == 7) { - readType = 5; - } - switch (readType) { - case 0: - case 3: - case 5: - assessOutlierMateDistance(); - if (distantMate == 1 && readType != 5) { - readType = 4; - } - break; - default: - break; - } - for (auto mpos_cit = samLine.cbegin() + 1 + samChunkPositions[6]; - mpos_cit != samLine.cbegin() + samChunkPositions[7]; ++mpos_cit) { - matePos = matePos * 10 + (*mpos_cit - '0'); - } - if (samLine[1 + samChunkPositions[5]] == '=') { - mateChrIndex = chrIndex; - } else { - mateChrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex( - next(samLine.cbegin(), 1 + samChunkPositions[5]), '\t'); - } -} - -void -Alignment::mappingQualityCheck() { - if (samLine[1 + samChunkPositions[3]] != - '0') { // mapq 0 is treated as a special case, where number of SAs and - // base qualities will be the sole determinants of read quality - nullMapq = false; - switch (samChunkPositions[4] - samChunkPositions[3]) { - case 2: // this checks if the mapq is a single-digit number - readType = 7; - lowMapq = true; - break; + switch (readType) { + case 0: case 3: - if (samLine[1 + samChunkPositions[3]] == '1' && - ((samLine[2 + samChunkPositions[3]] - '0') < 3)) { - // this checks if the mapq is a two-digit number, and if the - // first digit is a "1", that the second digit is less than 3 - readType = 7; - lowMapq = true; + case 5: + assessOutlierMateDistance(); + if (distantMate == 1 && readType != 5) { + readType = 4; } break; default: break; } + for (auto mpos_cit = samLine.cbegin() + 1 + samChunkPositions[6]; + mpos_cit != samLine.cbegin() + samChunkPositions[7]; ++mpos_cit) { + matePos = matePos * 10 + (*mpos_cit - '0'); + } + if (samLine[1 + samChunkPositions[5]] == '=') { + mateChrIndex = chrIndex; + } else { + mateChrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex( + next(samLine.cbegin(), 1 + samChunkPositions[5]), '\t'); + } } -} -bool -Alignment::isEventCandidate() const { - if (samLine[samChunkPositions[5] - 1] != 'M') { - return true; - } else { - for (auto cigarString_cit = samLine.cbegin() + 1 + samChunkPositions[4]; - cigarString_cit != samLine.cbegin() + samChunkPositions[5] - 1; - ++cigarString_cit) { - switch (*cigarString_cit) { - case 'S': - case 'H': - case 'I': - case 'D': - return true; + void + Alignment::mappingQualityCheck() { + if (samLine[1 + samChunkPositions[3]] != + '0') { // mapq 0 is treated as a special case, where number of SAs and + // base qualities will be the sole determinants of read quality + nullMapq = false; + switch (samChunkPositions[4] - samChunkPositions[3]) { + case 2: // this checks if the mapq is a single-digit number + readType = 7; + lowMapq = true; + break; + case 3: + if (samLine[1 + samChunkPositions[3]] == '1' && + ((samLine[2 + samChunkPositions[3]] - '0') < 3)) { + // this checks if the mapq is a two-digit number, and if the + // first digit is a "1", that the second digit is less than 3 + readType = 7; + lowMapq = true; + } + break; default: break; } } - return false; } -} -void -Alignment::createCigarChunks() { - auto encounteredM = false; - auto cumulativeNucleotideCount = 0, currentNucleotideCount = 0, - indelAdjustment = 0, leftClipAdjustment = 0, rightClipAdjustment = 0; - for (auto cigarString_cit = samLine.cbegin() + 1 + samChunkPositions[4]; - cigarString_cit != samLine.cbegin() + samChunkPositions[5]; - ++cigarString_cit) { - if (isdigit(*cigarString_cit)) { - currentNucleotideCount = - currentNucleotideCount * 10 + (*cigarString_cit - '0'); + bool + Alignment::isEventCandidate() const { + if (samLine[samChunkPositions[5] - 1] != 'M') { + return true; } else { - switch (*cigarString_cit) { - case 'M': - encounteredM = true; - cumulativeNucleotideCount += currentNucleotideCount; - break; + for (auto cigarString_cit = samLine.cbegin() + 1 + samChunkPositions[4]; + cigarString_cit != samLine.cbegin() + samChunkPositions[5] - 1; + ++cigarString_cit) { + switch (*cigarString_cit) { + case 'S': + case 'H': + case 'I': + case 'D': + return true; + default: + break; + } + } + return false; + } + } + + void + Alignment::createCigarChunks() { + auto encounteredM = false; + auto cumulativeNucleotideCount = 0, currentNucleotideCount = 0, + indelAdjustment = 0, leftClipAdjustment = 0, rightClipAdjustment = 0; + for (auto cigarString_cit = samLine.cbegin() + 1 + samChunkPositions[4]; + cigarString_cit != samLine.cbegin() + samChunkPositions[5]; + ++cigarString_cit) { + if (isdigit(*cigarString_cit)) { + currentNucleotideCount = + currentNucleotideCount * 10 + (*cigarString_cit - '0'); + } else { + switch (*cigarString_cit) { + case 'M': + encounteredM = true; + cumulativeNucleotideCount += currentNucleotideCount; + break; + case 'S': + cigarChunks.emplace_back(*cigarString_cit, encounteredM, + cumulativeNucleotideCount + + indelAdjustment - + leftClipAdjustment, + currentNucleotideCount, + indelAdjustment - leftClipAdjustment); + cumulativeNucleotideCount += currentNucleotideCount; + if (!encounteredM) { + leftClipAdjustment = currentNucleotideCount; + } else { + rightClipAdjustment = currentNucleotideCount; + } + break; + case 'H': + cigarChunks.emplace_back(*cigarString_cit, encounteredM, + cumulativeNucleotideCount + + indelAdjustment - + leftClipAdjustment, + currentNucleotideCount); + break; + case 'I': + cigarChunks.emplace_back(*cigarString_cit, encounteredM, + cumulativeNucleotideCount + + indelAdjustment - + leftClipAdjustment, + currentNucleotideCount); + cumulativeNucleotideCount += currentNucleotideCount; + indelAdjustment -= currentNucleotideCount; + break; + case 'D': + cigarChunks.emplace_back(*cigarString_cit, encounteredM, + cumulativeNucleotideCount + + indelAdjustment - + leftClipAdjustment, + currentNucleotideCount); + indelAdjustment += currentNucleotideCount; + break; + default: + break; + } + currentNucleotideCount = 0; + } + } + endPos += indelAdjustment - leftClipAdjustment - rightClipAdjustment; + } + + void + Alignment::assignBreakpointsAndOverhangs() { + for (const auto &chunk : cigarChunks) { + switch (chunk.chunkType) { case 'S': - cigarChunks.emplace_back(*cigarString_cit, encounteredM, - cumulativeNucleotideCount + - indelAdjustment - - leftClipAdjustment, - currentNucleotideCount, - indelAdjustment - leftClipAdjustment); - cumulativeNucleotideCount += currentNucleotideCount; - if (!encounteredM) { - leftClipAdjustment = currentNucleotideCount; + readBreakpointTypes.push_back(chunk.chunkType); + readBreakpointSizes.push_back(chunk.length); + readBreakpointsEncounteredM.push_back(chunk.encounteredM); + if (chunk.encounteredM) { + readBreakpoints.push_back(endPos); + readOverhangCoords.emplace_back( + chunk.encounteredM, endPos, + chunk.startPosOnRead - chunk.indelAdjustment, chunk.length); } else { - rightClipAdjustment = currentNucleotideCount; + readBreakpoints.push_back(startPos); + readOverhangCoords.emplace_back( + chunk.encounteredM, startPos, + chunk.startPosOnRead - chunk.indelAdjustment, chunk.length); } break; case 'H': - cigarChunks.emplace_back(*cigarString_cit, encounteredM, - cumulativeNucleotideCount + - indelAdjustment - - leftClipAdjustment, - currentNucleotideCount); + if (chunk.encounteredM) { + readBreakpoints.push_back(endPos); + } else { + readBreakpoints.push_back(startPos); + } + readBreakpointSizes.push_back(chunk.length); + readBreakpointTypes.push_back(chunk.chunkType); + readBreakpointsEncounteredM.push_back(chunk.encounteredM); break; case 'I': - cigarChunks.emplace_back(*cigarString_cit, encounteredM, - cumulativeNucleotideCount + - indelAdjustment - - leftClipAdjustment, - currentNucleotideCount); - cumulativeNucleotideCount += currentNucleotideCount; - indelAdjustment -= currentNucleotideCount; + readBreakpoints.push_back(startPos + chunk.startPosOnRead); + readBreakpointTypes.push_back(chunk.chunkType); + readBreakpointSizes.push_back(chunk.length); + readBreakpointsEncounteredM.push_back(chunk.encounteredM); break; case 'D': - cigarChunks.emplace_back(*cigarString_cit, encounteredM, - cumulativeNucleotideCount + - indelAdjustment - - leftClipAdjustment, - currentNucleotideCount); - indelAdjustment += currentNucleotideCount; + readBreakpoints.push_back(startPos + chunk.startPosOnRead); + readBreakpointTypes.push_back(chunk.chunkType); + readBreakpointSizes.push_back(chunk.length); + readBreakpointsEncounteredM.push_back(chunk.encounteredM); + readBreakpoints.push_back(startPos + chunk.startPosOnRead + + chunk.length); + readBreakpointSizes.push_back(-1); + readBreakpointTypes.push_back('#'); + readBreakpointsEncounteredM.push_back(chunk.encounteredM); break; default: break; } - currentNucleotideCount = 0; } } - endPos += indelAdjustment - leftClipAdjustment - rightClipAdjustment; -} -void -Alignment::assignBreakpointsAndOverhangs() { - for (const auto &chunk : cigarChunks) { - switch (chunk.chunkType) { - case 'S': - readBreakpointTypes.push_back(chunk.chunkType); - readBreakpointSizes.push_back(chunk.length); - readBreakpointsEncounteredM.push_back(chunk.encounteredM); - if (chunk.encounteredM) { - readBreakpoints.push_back(endPos); - readOverhangCoords.emplace_back( - chunk.encounteredM, endPos, - chunk.startPosOnRead - chunk.indelAdjustment, chunk.length); - } else { - readBreakpoints.push_back(startPos); - readOverhangCoords.emplace_back( - chunk.encounteredM, startPos, - chunk.startPosOnRead - chunk.indelAdjustment, chunk.length); - } - break; - case 'H': - if (chunk.encounteredM) { - readBreakpoints.push_back(endPos); - } else { - readBreakpoints.push_back(startPos); + void + Alignment::qualityCheckCascade() { + // cerr << "a\n"; + if (!clipCountCheck()) { + readType = 5; + return; + } + // cerr << "b\n"; + if (!uniqueSuppCheck()) { + readType = 5; + return; + } + // cerr << "c\n"; + if (!qualChecked) { + for (const auto &cigarChunk : cigarChunks) { + if (cigarChunk.chunkType == 'S' && + cigarChunk.length > LOWQUALCLIPTHRESHOLD && + overhangMedianQuality(cigarChunk) < BASEQUALITYTHRESHOLD) { + readType = 5; + return; + } } - readBreakpointSizes.push_back(chunk.length); - readBreakpointTypes.push_back(chunk.chunkType); - readBreakpointsEncounteredM.push_back(chunk.encounteredM); - break; - case 'I': - readBreakpoints.push_back(startPos + chunk.startPosOnRead); - readBreakpointTypes.push_back(chunk.chunkType); - readBreakpointSizes.push_back(chunk.length); - readBreakpointsEncounteredM.push_back(chunk.encounteredM); - break; - case 'D': - readBreakpoints.push_back(startPos + chunk.startPosOnRead); - readBreakpointTypes.push_back(chunk.chunkType); - readBreakpointSizes.push_back(chunk.length); - readBreakpointsEncounteredM.push_back(chunk.encounteredM); - readBreakpoints.push_back(startPos + chunk.startPosOnRead + - chunk.length); - readBreakpointSizes.push_back(-1); - readBreakpointTypes.push_back('#'); - readBreakpointsEncounteredM.push_back(chunk.encounteredM); - break; - default: - break; } + // cerr << "d\n"; + assessReadType(); + // cerr << "e\n"; } -} -void -Alignment::qualityCheckCascade() { - // cerr << "a\n"; - if (!clipCountCheck()) { - readType = 5; - return; - } - // cerr << "b\n"; - if (!uniqueSuppCheck()) { - readType = 5; - return; - } - // cerr << "c\n"; - if (!qualChecked) { + bool + Alignment::clipCountCheck() { + auto hCounts = 0; + auto sCounts = 0; for (const auto &cigarChunk : cigarChunks) { - if (cigarChunk.chunkType == 'S' && - cigarChunk.length > LOWQUALCLIPTHRESHOLD && - overhangMedianQuality(cigarChunk) < BASEQUALITYTHRESHOLD) { - readType = 5; - return; + switch (cigarChunk.chunkType) { + case 'H': + ++hCounts; + break; + case 'S': + ++sCounts; + break; + default: + break; } } - } - // cerr << "d\n"; - assessReadType(); - // cerr << "e\n"; -} - -bool -Alignment::clipCountCheck() { - auto hCounts = 0; - auto sCounts = 0; - for (const auto &cigarChunk : cigarChunks) { - switch (cigarChunk.chunkType) { - case 'H': - ++hCounts; - break; - case 'S': - ++sCounts; - break; - default: - break; + if (hCounts + sCounts > 1 && nullMapq) { + lowMapq = true; + return false; } + return (hCounts < 2 && !(hCounts > 0 && sCounts > 0)); } - if (hCounts + sCounts > 1 && nullMapq) { - lowMapq = true; - return false; - } - return (hCounts < 2 && !(hCounts > 0 && sCounts > 0)); -} -bool -Alignment::uniqueSuppCheck() { - auto hCounts = 0, sCounts = 0; - for (const auto &cigarChunk : cigarChunks) { - switch (cigarChunk.chunkType) { - case 'H': - ++hCounts; - break; - case 'S': - ++sCounts; - break; - default: - break; - } - } - saCbegin = samLine.cend(); - saCend = samLine.cend(); - if (samLine.back() == ';' && samLine[samChunkPositions.back() + 1] == 'S' && - samLine[samChunkPositions.back() + 2] == 'A') { - saCbegin = samLine.cbegin() + samChunkPositions.back() + 6; - saCend = samLine.cend() - 1; - hasSa = true; - } else { - for (auto i = 10u; i < samChunkPositions.size() - 1; ++i) { - if (samLine[samChunkPositions[i + 1] - 1] == ';' && - samLine[samChunkPositions[i] + 1] == 'S' && - samLine[samChunkPositions[i] + 2] == 'A') { - saCbegin = samLine.cbegin() + samChunkPositions[i] + 6; - saCend = samLine.cbegin() + samChunkPositions[i + 1] - 1; - hasSa = true; + bool + Alignment::uniqueSuppCheck() { + auto hCounts = 0, sCounts = 0; + for (const auto &cigarChunk : cigarChunks) { + switch (cigarChunk.chunkType) { + case 'H': + ++hCounts; + break; + case 'S': + ++sCounts; + break; + default: break; } } - } - if (hasSa) { - auto lowQualSacounts = 0; - auto block = 0; - auto mapq = 0; - auto highQualSa = false; - for (auto saCit = saCbegin; saCit != saCend; ++saCit) { - //"SA:Z:10,24753146,+,68S33M,48,1;X,135742083,-,47S22M32S,0,0;8,72637925,-,29S19M53S,0,0;" - // 0-chr,1-pos,2-orientation,3-cigar,4-mapq,5-whatever - switch (*saCit) { - case ',': - ++block; - if (block == 4) { - ++saCit; - while (*saCit != ',') { - mapq = mapq * 10 + (*saCit - '0'); + saCbegin = samLine.cend(); + saCend = samLine.cend(); + if (samLine.back() == ';' && samLine[samChunkPositions.back() + 1] == 'S' && + samLine[samChunkPositions.back() + 2] == 'A') { + saCbegin = samLine.cbegin() + samChunkPositions.back() + 6; + saCend = samLine.cend() - 1; + hasSa = true; + } else { + for (auto i = 10u; i < samChunkPositions.size() - 1; ++i) { + if (samLine[samChunkPositions[i + 1] - 1] == ';' && + samLine[samChunkPositions[i] + 1] == 'S' && + samLine[samChunkPositions[i] + 2] == 'A') { + saCbegin = samLine.cbegin() + samChunkPositions[i] + 6; + saCend = samLine.cbegin() + samChunkPositions[i + 1] - 1; + hasSa = true; + break; + } + } + } + if (hasSa) { + auto lowQualSacounts = 0; + auto block = 0; + auto mapq = 0; + auto highQualSa = false; + for (auto saCit = saCbegin; saCit != saCend; ++saCit) { + //"SA:Z:10,24753146,+,68S33M,48,1;X,135742083,-,47S22M32S,0,0;8,72637925,-,29S19M53S,0,0;" + // 0-chr,1-pos,2-orientation,3-cigar,4-mapq,5-whatever + switch (*saCit) { + case ',': + ++block; + if (block == 4) { ++saCit; + while (*saCit != ',') { + mapq = mapq * 10 + (*saCit - '0'); + ++saCit; + } } + break; + case ';': + if (mapq < 13) { + ++lowQualSacounts; + } else if (mapq > 20) { + highQualSa = true; + } + if (!highQualSa && ((sCounts == 1 && lowQualSacounts == 2) || + (hCounts == 1 && lowQualSacounts == 2) || + (sCounts == 2 && lowQualSacounts == 4))) { + return false; + } + block = 0; + mapq = 0; + break; + default: + break; } - break; - case ';': - if (mapq < 13) { - ++lowQualSacounts; - } else if (mapq > 20) { - highQualSa = true; - } - if (!highQualSa && ((sCounts == 1 && lowQualSacounts == 2) || - (hCounts == 1 && lowQualSacounts == 2) || - (sCounts == 2 && lowQualSacounts == 4))) { - return false; - } - block = 0; - mapq = 0; - break; - default: - break; } } + return true; } - return true; -} -double -Alignment::overhangMedianQuality(const CigarChunk &cigarChunk) const { - vector overhangPerBaseQuality{}; - if (!cigarChunk.encounteredM) { - auto startCit = next(samLine.cbegin(), 1 + samChunkPositions[9] + - cigarChunk.startPosOnRead - - cigarChunk.indelAdjustment); - auto endCit = - next(samLine.cbegin(), - 1 + samChunkPositions[9] + cigarChunk.startPosOnRead - - cigarChunk.indelAdjustment + cigarChunk.length); - fullMedianQuality(startCit, endCit, overhangPerBaseQuality); - } else { - string::const_reverse_iterator startCrit{ - next(samLine.cbegin(), - 1 + samChunkPositions[9] + cigarChunk.startPosOnRead - - cigarChunk.indelAdjustment + cigarChunk.length)}; - string::const_reverse_iterator endCrit{ - next(samLine.cbegin(), 1 + samChunkPositions[9] + - cigarChunk.startPosOnRead - - cigarChunk.indelAdjustment)}; - fullMedianQuality(startCrit, endCrit, overhangPerBaseQuality); - } - if (overhangPerBaseQuality.empty()) { - return -1.0; - } else { - return getMedian(overhangPerBaseQuality.begin(), - overhangPerBaseQuality.end()); + double + Alignment::overhangMedianQuality(const CigarChunk &cigarChunk) const { + vector overhangPerBaseQuality{}; + if (!cigarChunk.encounteredM) { + auto startCit = next(samLine.cbegin(), 1 + samChunkPositions[9] + + cigarChunk.startPosOnRead - + cigarChunk.indelAdjustment); + auto endCit = + next(samLine.cbegin(), + 1 + samChunkPositions[9] + cigarChunk.startPosOnRead - + cigarChunk.indelAdjustment + cigarChunk.length); + fullMedianQuality(startCit, endCit, overhangPerBaseQuality); + } else { + string::const_reverse_iterator startCrit{ + next(samLine.cbegin(), + 1 + samChunkPositions[9] + cigarChunk.startPosOnRead - + cigarChunk.indelAdjustment + cigarChunk.length)}; + string::const_reverse_iterator endCrit{ + next(samLine.cbegin(), 1 + samChunkPositions[9] + + cigarChunk.startPosOnRead - + cigarChunk.indelAdjustment)}; + fullMedianQuality(startCrit, endCrit, overhangPerBaseQuality); + } + if (overhangPerBaseQuality.empty()) { + return -1.0; + } else { + return getMedian(overhangPerBaseQuality.begin(), + overhangPerBaseQuality.end()); + } } -} -void -Alignment::assessReadType() { - /* 0 for non-split, - * 1 for softSplit, - * 2 for hardSplit, - * 3 for indel, - * 4 for distant mate, - * 5 for low quality overhang - * 6 for low quality hardClipped - * (precedence: - * decoy mate> - * low qual > - * soft clips > - * hard clips > - * distant mate > - * indels > - * normal) - */ - auto hardLongClip = false, indelStatus = false; - for (const auto &chunk : cigarChunks) { - switch (chunk.chunkType) { - case 'S': - if (chunk.length >= CLIPPEDNUCLEOTIDECOUNTTHRESHOLD) { - readType = 1; - return; - } - break; - case 'H': - if (chunk.length >= CLIPPEDNUCLEOTIDECOUNTTHRESHOLD) { - hardLongClip = true; + void + Alignment::assessReadType() { + /* 0 for non-split, + * 1 for softSplit, + * 2 for hardSplit, + * 3 for indel, + * 4 for distant mate, + * 5 for low quality overhang + * 6 for low quality hardClipped + * (precedence: + * decoy mate> + * low qual > + * soft clips > + * hard clips > + * distant mate > + * indels > + * normal) + */ + auto hardLongClip = false, indelStatus = false; + for (const auto &chunk : cigarChunks) { + switch (chunk.chunkType) { + case 'S': + if (chunk.length >= CLIPPEDNUCLEOTIDECOUNTTHRESHOLD) { + readType = 1; + return; + } + break; + case 'H': + if (chunk.length >= CLIPPEDNUCLEOTIDECOUNTTHRESHOLD) { + hardLongClip = true; + } + break; + case 'I': + case 'D': + indelStatus = true; + break; + default: + break; } - break; - case 'I': - case 'D': - indelStatus = true; - break; - default: - break; + } + if (hardLongClip) { + readType = 2; + } else if (indelStatus) { + readType = 3; } } - if (hardLongClip) { - readType = 2; - } else if (indelStatus) { - readType = 3; - } -} -bool -Alignment::assessOutlierMateDistance() { - switch (distantMate) { - case -1: - return false; - case 1: - return true; - default: - if (*(samLine.cbegin() + 1 + samChunkPositions[5]) != '=') { - distantMate = 1; + bool + Alignment::assessOutlierMateDistance() { + switch (distantMate) { + case -1: + return false; + case 1: return true; - } else { - auto isize_cit = samLine.cbegin() + 1 + samChunkPositions[7]; - if (*isize_cit == '-') { - ++isize_cit; - } - auto isize = 0; - for (; isize_cit != samLine.cbegin() + samChunkPositions[8]; - ++isize_cit) { - isize = isize * 10 + (*isize_cit - '0'); - } - if (isize > ISIZEMAX) { + default: + if (*(samLine.cbegin() + 1 + samChunkPositions[5]) != '=') { distantMate = 1; return true; + } else { + auto isize_cit = samLine.cbegin() + 1 + samChunkPositions[7]; + if (*isize_cit == '-') { + ++isize_cit; + } + auto isize = 0; + for (; isize_cit != samLine.cbegin() + samChunkPositions[8]; + ++isize_cit) { + isize = isize * 10 + (*isize_cit - '0'); + } + if (isize > ISIZEMAX) { + distantMate = 1; + return true; + } } + distantMate = -1; + return false; } - distantMate = -1; - return false; } -} -void -Alignment::setChosenBp(int chosenBpLoc, int alignmentIndex) { - auto overhangStartIndex = 0; - auto overhangLength = 0; - char bpType{}; - auto bpEncounteredM = false; - auto bpSize = 0; - for (auto i = 0u; i < readBreakpoints.size(); ++i) { - if (readBreakpoints[i] == chosenBpLoc) { - bpEncounteredM = readBreakpointsEncounteredM[i]; - bpType = readBreakpointTypes[i]; - if (bpType == 'S') { - for (const auto &overhang : readOverhangCoords) { - if (overhang.bpPos == chosenBpLoc) { - overhangStartIndex = - 1 + samChunkPositions[8] + overhang.startPosOnRead; - overhangLength = overhang.length; - break; + void + Alignment::setChosenBp(int chosenBpLoc, int alignmentIndex) { + auto overhangStartIndex = 0; + auto overhangLength = 0; + char bpType{}; + auto bpEncounteredM = false; + auto bpSize = 0; + for (auto i = 0u; i < readBreakpoints.size(); ++i) { + if (readBreakpoints[i] == chosenBpLoc) { + bpEncounteredM = readBreakpointsEncounteredM[i]; + bpType = readBreakpointTypes[i]; + if (bpType == 'S') { + for (const auto &overhang : readOverhangCoords) { + if (overhang.bpPos == chosenBpLoc) { + overhangStartIndex = + 1 + samChunkPositions[8] + overhang.startPosOnRead; + overhangLength = overhang.length; + break; + } } } + bpSize = readBreakpointSizes[i]; + break; } - bpSize = readBreakpointSizes[i]; - break; } - } - chosenBp.reset(); - chosenBp = make_unique(bpType, bpSize, bpEncounteredM, - overhangStartIndex, overhangLength, - alignmentIndex); -} -vector -Alignment::generateSuppAlignments(int bpChrIndex, int bpPos) { - vector suppAlignmentsTmp; - if (hasSa) { - vector saBegins = {saCbegin}; - vector saEnds; - for (auto it = saCbegin; it != saCend; ++it) { - if (*it == ';') { - saEnds.push_back(it); - saBegins.push_back(it + 1); + chosenBp.reset(); + chosenBp = make_unique(bpType, bpSize, bpEncounteredM, + overhangStartIndex, overhangLength, + alignmentIndex); + } + vector + Alignment::generateSuppAlignments(int bpChrIndex, int bpPos) { + vector suppAlignmentsTmp; + if (hasSa) { + vector saBegins = {saCbegin}; + vector saEnds; + for (auto it = saCbegin; it != saCend; ++it) { + if (*it == ';') { + saEnds.push_back(it); + saBegins.push_back(it + 1); + } } - } - saEnds.push_back(saCend); - for (auto i = 0u; i < saBegins.size(); ++i) { - SuppAlignment saTmp{saBegins[i], - saEnds[i], - !supplementary, - lowMapq, - nullMapq, - fwdStrand, - chosenBp->bpEncounteredM, - chosenBp->selfNodeIndex, - bpChrIndex, - bpPos}; - if (saTmp.getChrIndex() < 1002) { - suppAlignmentsTmp.push_back(saTmp); + saEnds.push_back(saCend); + for (auto i = 0u; i < saBegins.size(); ++i) { + SuppAlignment saTmp{saBegins[i], + saEnds[i], + !supplementary, + lowMapq, + nullMapq, + fwdStrand, + chosenBp->bpEncounteredM, + chosenBp->selfNodeIndex, + bpChrIndex, + bpPos}; + if (saTmp.getChrIndex() < 1002) { + suppAlignmentsTmp.push_back(saTmp); + } } } - } - if (assessOutlierMateDistance()) { - if (getMateChrIndex() < 1002) { - auto foundMatch = false; - MateInfo tmpPairDummy{ - 0, 0, getMateChrIndex(), getMatePos(), true, invertedMate}; - for (const auto &sa : suppAlignmentsTmp) { - if (tmpPairDummy.suppAlignmentFuzzyMatch(sa)) { - foundMatch = true; - break; + if (assessOutlierMateDistance()) { + if (getMateChrIndex() < 1002) { + auto foundMatch = false; + MateInfo tmpPairDummy{ + 0, 0, getMateChrIndex(), getMatePos(), true, invertedMate}; + for (const auto &sa : suppAlignmentsTmp) { + if (tmpPairDummy.suppAlignmentFuzzyMatch(sa)) { + foundMatch = true; + break; + } + } + if (!foundMatch) { + suppAlignmentsTmp.emplace_back( + getMateChrIndex(), getMatePos(), 0, 0, + chosenBp->bpEncounteredM, invertedMate, getMatePos() + 1, + !supplementary, lowMapq, nullMapq, chosenBp->selfNodeIndex); } - } - if (!foundMatch) { - suppAlignmentsTmp.emplace_back( - getMateChrIndex(), getMatePos(), 0, 0, - chosenBp->bpEncounteredM, invertedMate, getMatePos() + 1, - !supplementary, lowMapq, nullMapq, chosenBp->selfNodeIndex); } } + return suppAlignmentsTmp; + } + string + Alignment::printOverhang() const { + string res{}; + res.reserve(chosenBp->overhangLength + 9); + if (chosenBp->bpEncounteredM) { + res.append("|").append(samLine.substr(chosenBp->overhangStartIndex, + chosenBp->overhangLength)); + } else { + res.append(samLine.substr(chosenBp->overhangStartIndex, + chosenBp->overhangLength)) + .append("|"); + } + res.append("(") + .append(strtk::type_to_string(chosenBp->childrenNodes.size())) + .append(")"); + return res; } - return suppAlignmentsTmp; -} -string -Alignment::printOverhang() const { - string res{}; - res.reserve(chosenBp->overhangLength + 9); - if (chosenBp->bpEncounteredM) { - res.append("|").append(samLine.substr(chosenBp->overhangStartIndex, - chosenBp->overhangLength)); - } else { - res.append(samLine.substr(chosenBp->overhangStartIndex, - chosenBp->overhangLength)) - .append("|"); - } - res.append("(") - .append(strtk::type_to_string(chosenBp->childrenNodes.size())) - .append(")"); - return res; -} -double -Alignment::overhangComplexityMaskRatio() const { - auto fullSizesTotal = 0.0; - auto maskedIntervalsTotal = 0.0; - vector overhang; - for (auto i = 0; i < chosenBp->overhangLength; ++i) { - switch (samLine[chosenBp->overhangStartIndex + i]) { - case 'A': - overhang.push_back(0); - break; - case 'T': - overhang.push_back(1); - break; - case 'G': - overhang.push_back(2); - break; - case 'C': - overhang.push_back(3); - break; - case 'N': - if (!overhang.empty()) { - fullSizesTotal += overhang.size(); - auto res = Sdust{overhang}.getRes(); - if (!res.empty()) { - for (const auto &resInterval : res) { - maskedIntervalsTotal += - resInterval.endIndex - resInterval.startIndex + 1; + double + Alignment::overhangComplexityMaskRatio() const { + auto fullSizesTotal = 0.0; + auto maskedIntervalsTotal = 0.0; + vector overhang; + for (auto i = 0; i < chosenBp->overhangLength; ++i) { + switch (samLine[chosenBp->overhangStartIndex + i]) { + case 'A': + overhang.push_back(0); + break; + case 'T': + overhang.push_back(1); + break; + case 'G': + overhang.push_back(2); + break; + case 'C': + overhang.push_back(3); + break; + case 'N': + if (!overhang.empty()) { + fullSizesTotal += overhang.size(); + auto res = Sdust{overhang}.getRes(); + if (!res.empty()) { + for (const auto &resInterval : res) { + maskedIntervalsTotal += + resInterval.endIndex - resInterval.startIndex + 1; + } } + overhang.clear(); } - overhang.clear(); + break; + default: + break; } - break; - default: - break; } - } - if (!overhang.empty()) { - fullSizesTotal += overhang.size(); - auto res = Sdust{overhang}.getRes(); - if (!res.empty()) { - for (const auto &resInterval : res) { - maskedIntervalsTotal += - resInterval.endIndex - resInterval.startIndex + 1; + if (!overhang.empty()) { + fullSizesTotal += overhang.size(); + auto res = Sdust{overhang}.getRes(); + if (!res.empty()) { + for (const auto &resInterval : res) { + maskedIntervalsTotal += + resInterval.endIndex - resInterval.startIndex + 1; + } } } + return maskedIntervalsTotal / fullSizesTotal; } - return maskedIntervalsTotal / fullSizesTotal; -} -template -void -Alignment::fullMedianQuality(Iterator qualBegin, Iterator qualEnd, - vector &overhangPerBaseQuality) const { - overhangPerBaseQuality.reserve(distance(qualBegin, qualEnd)); - auto consecutiveLowQuals = 0; - for (auto cit = qualBegin; cit != qualEnd; ++cit) { - if (*cit < BASEQUALITYTHRESHOLDLOW) { // 33 + phred 11 - if (consecutiveLowQuals == 5) { - overhangPerBaseQuality.clear(); - return; + template + void + Alignment::fullMedianQuality(Iterator qualBegin, Iterator qualEnd, + vector &overhangPerBaseQuality) const { + overhangPerBaseQuality.reserve(distance(qualBegin, qualEnd)); + auto consecutiveLowQuals = 0; + for (auto cit = qualBegin; cit != qualEnd; ++cit) { + if (*cit < BASEQUALITYTHRESHOLDLOW) { // 33 + phred 11 + if (consecutiveLowQuals == 5) { + overhangPerBaseQuality.clear(); + return; + } + ++consecutiveLowQuals; + } else { + consecutiveLowQuals = 0; } - ++consecutiveLowQuals; - } else { - consecutiveLowQuals = 0; + overhangPerBaseQuality.push_back(*cit); } - overhangPerBaseQuality.push_back(*cit); } -} -// Median Code taken from http://rosettacode.org/wiki/Averages/Median#C.2B.2B -template -double -Alignment::getMedian(Iterator begin, Iterator end) const { - // this is middle for odd-length, and "upper-middle" for even length - Iterator middle = begin + (end - begin) / 2; - // This function runs in O(n) on average, according to the standard - nth_element(begin, middle, end); - if ((end - begin) % 2 != 0) { // odd length - return *middle; - } else { // even length - // the "lower middle" is the max of the lower half - Iterator lower_middle = max_element(begin, middle); - return (*middle + *lower_middle) / 2.0; + // Median Code taken from http://rosettacode.org/wiki/Averages/Median#C.2B.2B + template + double + Alignment::getMedian(Iterator begin, Iterator end) const { + // this is middle for odd-length, and "upper-middle" for even length + Iterator middle = begin + (end - begin) / 2; + // This function runs in O(n) on average, according to the standard + nth_element(begin, middle, end); + if ((end - begin) % 2 != 0) { // odd length + return *middle; + } else { // even length + // the "lower middle" is the max of the lower half + Iterator lower_middle = max_element(begin, middle); + return (*middle + *lower_middle) / 2.0; + } } -} } /* namespace sophia */ diff --git a/src/AnnotationProcessor.cpp b/src/AnnotationProcessor.cpp index 0a80b08..62dbc4f 100644 --- a/src/AnnotationProcessor.cpp +++ b/src/AnnotationProcessor.cpp @@ -37,387 +37,427 @@ namespace sophia { -using namespace std; + bool AnnotationProcessor::ABRIDGEDOUTPUT{false}; -bool AnnotationProcessor::ABRIDGEDOUTPUT{false}; - -AnnotationProcessor::AnnotationProcessor(const string &tumorResultsIn, - vector> &mref, - int defaultReadLengthTumorIn, - bool controlCheckMode, - int germlineDbLimit) - : NOCONTROLMODE{true}, - GERMLINEDBLIMIT{germlineDbLimit}, - contaminationObserved{false}, - massiveInvFilteringLevel{0}, - filteredResults{}, - tumorResults{85, vector{}}, - controlResults{85, vector{}}, - visitedLineIndices{} { - unique_ptr tumorInputHandle{ - make_unique(tumorResultsIn, ios_base::in | ios_base::binary)}; - unique_ptr tumorGzHandle{ - make_unique()}; - tumorGzHandle->push(boost::iostreams::gzip_decompressor()); - tumorGzHandle->push(*tumorInputHandle); - string line; - auto lineIndex = 0; - const ChrConverter& chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - while (error_terminating_getline(*tumorGzHandle, line)) { - if (line.front() == '#') { - continue; - }; - Breakpoint tmpBp{line, true}; - auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; - if (chrIndex < 0) { - continue; - } - auto hasOverhang = line.back() != '.' && line.back() != '#'; - tumorResults[chrIndex].emplace_back(tmpBp, lineIndex, hasOverhang); - if (hasOverhang) { - string overhang{}; - for (auto it = line.rbegin(); it != line.rend(); ++it) { - if (*it == '\t') { - break; - } else { - overhang.push_back(*it); + AnnotationProcessor::AnnotationProcessor(const string &tumorResultsIn, + vector> &mref, + int defaultReadLengthTumorIn, + bool controlCheckMode, + int germlineDbLimit) + : NOCONTROLMODE{true}, + GERMLINEDBLIMIT{germlineDbLimit}, + contaminationObserved{false}, + massiveInvFilteringLevel{0}, + filteredResults{}, + tumorResults{85, vector{}}, + controlResults{85, vector{}}, + visitedLineIndices{} { + unique_ptr tumorInputHandle{ + make_unique(tumorResultsIn, ios_base::in | ios_base::binary)}; + unique_ptr tumorGzHandle{ + make_unique()}; + tumorGzHandle->push(boost::iostreams::gzip_decompressor()); + tumorGzHandle->push(*tumorInputHandle); + string line; + auto lineIndex = 0; + const ChrConverter& chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + while (error_terminating_getline(*tumorGzHandle, line)) { + if (line.front() == '#') { + continue; + }; + Breakpoint tmpBp{line, true}; + auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; + if (chrIndex < 0) { + continue; + } + auto hasOverhang = line.back() != '.' && line.back() != '#'; + tumorResults[chrIndex].emplace_back(tmpBp, lineIndex, hasOverhang); + if (hasOverhang) { + string overhang{}; + for (auto it = line.rbegin(); it != line.rend(); ++it) { + if (*it == '\t') { + break; + } else { + overhang.push_back(*it); + } } + reverse(overhang.begin(), overhang.end()); + overhangs.emplace_back(lineIndex, overhang); + } else { + visitedLineIndices.push_back(lineIndex); } - reverse(overhang.begin(), overhang.end()); - overhangs.emplace_back(lineIndex, overhang); - } else { - visitedLineIndices.push_back(lineIndex); + ++lineIndex; } - ++lineIndex; - } - for (auto &tres : tumorResults) { - DeFuzzier deFuzzier{defaultReadLengthTumorIn * 6, false}; - deFuzzier.deFuzzyDb(tres); - } - searchMatches(mref); - if (applyMassiveInversionFiltering(false, controlCheckMode)) { - ++massiveInvFilteringLevel; - } - if (massiveInvFilteringLevel == 1) { - if (applyMassiveInversionFiltering(true, controlCheckMode)) { + for (auto &tres : tumorResults) { + DeFuzzier deFuzzier{defaultReadLengthTumorIn * 6, false}; + deFuzzier.deFuzzyDb(tres); + } + searchMatches(mref); + if (applyMassiveInversionFiltering(false, controlCheckMode)) { ++massiveInvFilteringLevel; } + if (massiveInvFilteringLevel == 1) { + if (applyMassiveInversionFiltering(true, controlCheckMode)) { + ++massiveInvFilteringLevel; + } + } + contaminationObserved = applyPathogenContaminationFiltering(); + if (!controlCheckMode && !contaminationObserved && + massiveInvFilteringLevel == 0) { + printUnresolvedRareOverhangs(mref); + } } - contaminationObserved = applyPathogenContaminationFiltering(); - if (!controlCheckMode && !contaminationObserved && - massiveInvFilteringLevel == 0) { - printUnresolvedRareOverhangs(mref); - } -} -AnnotationProcessor::AnnotationProcessor( - const string &tumorResultsIn, vector> &mref, - const string &controlResultsIn, int defaultReadLengthTumorIn, - int defaultReadLengthControlIn, int germlineDbLimit, int lowQualControlIn, - bool pathogenInControlIn) - : NOCONTROLMODE{false}, - GERMLINEDBLIMIT{germlineDbLimit}, - contaminationObserved{false}, - massiveInvFilteringLevel{0}, - filteredResults{}, - tumorResults{85, vector{}}, - controlResults{85, vector{}} { - unique_ptr controlInputHandle{make_unique( - controlResultsIn, ios_base::in | ios_base::binary)}; - unique_ptr controlGzHandle{ - make_unique()}; - controlGzHandle->push(boost::iostreams::gzip_decompressor()); - controlGzHandle->push(*controlInputHandle); - string line; - auto lineIndex = 0; - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - while (error_terminating_getline(*controlGzHandle, line)) { - if (line.front() == '#') { - continue; - }; - Breakpoint tmpBpPre{line, true}; - BreakpointReduced tmpBp{tmpBpPre, lineIndex, false}; - if (tmpBp.getChrIndex() > 1001) { - continue; - } - if (pathogenInControlIn) { - if ((tmpBp.getPairedBreaksSoft() + tmpBp.getUnpairedBreaksSoft()) > - 19 && - (tmpBp.getPairedBreaksHard() + tmpBp.getUnpairedBreaksHard() < - 3)) { - if (line.back() != '.' && line.back() != '#') { - string overhang{}; - auto overhangLength = 0; - auto maxOverhangLength = 0; - for (auto it = line.rbegin(); *it != '\t'; ++it) { - switch (*it) { - case '(': - overhangLength = 0; - break; - case ':': - maxOverhangLength = - max(maxOverhangLength, overhangLength); - overhangLength = 0; - break; - default: - ++overhangLength; - break; + AnnotationProcessor::AnnotationProcessor( + const string &tumorResultsIn, vector> &mref, + const string &controlResultsIn, int defaultReadLengthTumorIn, + int defaultReadLengthControlIn, int germlineDbLimit, int lowQualControlIn, + bool pathogenInControlIn) + : NOCONTROLMODE{false}, + GERMLINEDBLIMIT{germlineDbLimit}, + contaminationObserved{false}, + massiveInvFilteringLevel{0}, + filteredResults{}, + tumorResults{85, vector{}}, + controlResults{85, vector{}} { + unique_ptr controlInputHandle{make_unique( + controlResultsIn, ios_base::in | ios_base::binary)}; + unique_ptr controlGzHandle{ + make_unique()}; + controlGzHandle->push(boost::iostreams::gzip_decompressor()); + controlGzHandle->push(*controlInputHandle); + string line; + auto lineIndex = 0; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + while (error_terminating_getline(*controlGzHandle, line)) { + if (line.front() == '#') { + continue; + }; + Breakpoint tmpBpPre{line, true}; + BreakpointReduced tmpBp{tmpBpPre, lineIndex, false}; + if (tmpBp.getChrIndex() > 1001) { + continue; + } + if (pathogenInControlIn) { + if ((tmpBp.getPairedBreaksSoft() + tmpBp.getUnpairedBreaksSoft()) > + 19 && + (tmpBp.getPairedBreaksHard() + tmpBp.getUnpairedBreaksHard() < + 3)) { + if (line.back() != '.' && line.back() != '#') { + string overhang{}; + auto overhangLength = 0; + auto maxOverhangLength = 0; + for (auto it = line.rbegin(); *it != '\t'; ++it) { + switch (*it) { + case '(': + overhangLength = 0; + break; + case ':': + maxOverhangLength = + max(maxOverhangLength, overhangLength); + overhangLength = 0; + break; + default: + ++overhangLength; + break; + } + } + auto maxOverhangLengthRatio = + (maxOverhangLength + 0.0) / defaultReadLengthControlIn; + if (maxOverhangLengthRatio > 0.7) { + continue; } - } - auto maxOverhangLengthRatio = - (maxOverhangLength + 0.0) / defaultReadLengthControlIn; - if (maxOverhangLengthRatio > 0.7) { - continue; } } } - } - if (lowQualControlIn > 0) { - SuppAlignmentAnno *bestSa = nullptr; - auto bestSaSupport = 0; - if (!tmpBp.getSuppAlignments().empty()) { - for (const auto sa : tmpBp.getSupplementsPtr()) { - if (!sa->isSuspicious()) { - if (bestSa) { - auto saSupport = sa->getSupport() + - sa->getSecondarySupport() + - sa->getMateSupport(); - if (saSupport > bestSaSupport) { + if (lowQualControlIn > 0) { + SuppAlignmentAnno *bestSa = nullptr; + auto bestSaSupport = 0; + if (!tmpBp.getSuppAlignments().empty()) { + for (const auto sa : tmpBp.getSupplementsPtr()) { + if (!sa->isSuspicious()) { + if (bestSa) { + auto saSupport = sa->getSupport() + + sa->getSecondarySupport() + + sa->getMateSupport(); + if (saSupport > bestSaSupport) { + bestSa = sa; + bestSaSupport = saSupport; + } + } else { bestSa = sa; - bestSaSupport = saSupport; } - } else { - bestSa = sa; } } } - } - auto clipTotal = - tmpBp.getPairedBreaksHard() + tmpBp.getPairedBreaksSoft() + - tmpBp.getUnpairedBreaksHard() + tmpBp.getUnpairedBreaksSoft(); - if (!bestSa) { - if (clipTotal < 10) { - continue; - } - } else { - if (bestSa->isInverted()) { - auto suppDist = tmpBp.distanceToSupp(*bestSa); - if (suppDist > 0) { - if (suppDist < 10000) { - if (lowQualControlIn == 2) { - continue; + auto clipTotal = + tmpBp.getPairedBreaksHard() + tmpBp.getPairedBreaksSoft() + + tmpBp.getUnpairedBreaksHard() + tmpBp.getUnpairedBreaksSoft(); + if (!bestSa) { + if (clipTotal < 10) { + continue; + } + } else { + if (bestSa->isInverted()) { + auto suppDist = tmpBp.distanceToSupp(*bestSa); + if (suppDist > 0) { + if (suppDist < 10000) { + if (lowQualControlIn == 2) { + continue; + } else { + if (bestSa->getSupport() < 5 || + bestSa->getSecondarySupport() < 5) { + continue; + } + } } else { - if (bestSa->getSupport() < 5 || + if (bestSa->getSupport() < 5 && bestSa->getSecondarySupport() < 5) { continue; } } - } else { + } else if (suppDist < 0) { if (bestSa->getSupport() < 5 && bestSa->getSecondarySupport() < 5) { continue; } } - } else if (suppDist < 0) { - if (bestSa->getSupport() < 5 && - bestSa->getSecondarySupport() < 5) { - continue; - } } } } + auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; + controlResults[chrIndex].push_back(tmpBp); + ++lineIndex; } - auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; - controlResults[chrIndex].push_back(tmpBp); - ++lineIndex; - } - for (auto &cres : controlResults) { - DeFuzzier deFuzzierControl{defaultReadLengthControlIn * 6, false}; - deFuzzierControl.deFuzzyDb(cres); - } - unique_ptr tumorInputHandle{ - make_unique(tumorResultsIn, ios_base::in | ios_base::binary)}; - unique_ptr tumorGzHandle{ - make_unique()}; - tumorGzHandle->push(boost::iostreams::gzip_decompressor()); - tumorGzHandle->push(*tumorInputHandle); - lineIndex = 0; - while (error_terminating_getline(*tumorGzHandle, line)) { - if (line.front() == '#') { - continue; - }; - Breakpoint tmpBp{line, true}; - auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; - if (chrIndex < 0) { - continue; + for (auto &cres : controlResults) { + DeFuzzier deFuzzierControl{defaultReadLengthControlIn * 6, false}; + deFuzzierControl.deFuzzyDb(cres); } - auto hasOverhang = line.back() != '.' && line.back() != '#'; - tumorResults[chrIndex].emplace_back(tmpBp, lineIndex, hasOverhang); - if (line.back() != '.' && line.back() != '#') { - string overhang{}; - for (auto it = line.rbegin(); it != line.rend(); ++it) { - if (*it == '\t') { - break; - } else { - overhang.push_back(*it); + unique_ptr tumorInputHandle{ + make_unique(tumorResultsIn, ios_base::in | ios_base::binary)}; + unique_ptr tumorGzHandle{ + make_unique()}; + tumorGzHandle->push(boost::iostreams::gzip_decompressor()); + tumorGzHandle->push(*tumorInputHandle); + lineIndex = 0; + while (error_terminating_getline(*tumorGzHandle, line)) { + if (line.front() == '#') { + continue; + }; + Breakpoint tmpBp{line, true}; + auto chrIndex = chrConverter.indexConverter[tmpBp.getChrIndex()]; + if (chrIndex < 0) { + continue; + } + auto hasOverhang = line.back() != '.' && line.back() != '#'; + tumorResults[chrIndex].emplace_back(tmpBp, lineIndex, hasOverhang); + if (line.back() != '.' && line.back() != '#') { + string overhang{}; + for (auto it = line.rbegin(); it != line.rend(); ++it) { + if (*it == '\t') { + break; + } else { + overhang.push_back(*it); + } } + reverse(overhang.begin(), overhang.end()); + overhangs.emplace_back(lineIndex, overhang); + } else { + visitedLineIndices.push_back(lineIndex); } - reverse(overhang.begin(), overhang.end()); - overhangs.emplace_back(lineIndex, overhang); - } else { - visitedLineIndices.push_back(lineIndex); + ++lineIndex; } - ++lineIndex; - } - for (auto &tres : tumorResults) { - DeFuzzier deFuzzierTumor{defaultReadLengthTumorIn * 6, false}; - deFuzzierTumor.deFuzzyDb(tres); - } - searchMatches(mref); - if (applyMassiveInversionFiltering(false, false)) { - ++massiveInvFilteringLevel; - } - if (massiveInvFilteringLevel == 1) { - if (applyMassiveInversionFiltering(true, false)) { + for (auto &tres : tumorResults) { + DeFuzzier deFuzzierTumor{defaultReadLengthTumorIn * 6, false}; + deFuzzierTumor.deFuzzyDb(tres); + } + searchMatches(mref); + if (applyMassiveInversionFiltering(false, false)) { ++massiveInvFilteringLevel; } + if (massiveInvFilteringLevel == 1) { + if (applyMassiveInversionFiltering(true, false)) { + ++massiveInvFilteringLevel; + } + } + contaminationObserved = applyPathogenContaminationFiltering(); + if (!contaminationObserved && massiveInvFilteringLevel == 0) { + printUnresolvedRareOverhangs(mref); + } } - contaminationObserved = applyPathogenContaminationFiltering(); - if (!contaminationObserved && massiveInvFilteringLevel == 0) { - printUnresolvedRareOverhangs(mref); - } -} -void -AnnotationProcessor::searchMatches(vector> &mref) { - for (auto j = 0; j < 85; ++j) { - for (auto i = 0u; i < tumorResults[j].size(); ++i) { - for (const auto &sa : tumorResults[j][i].getSuppAlignments()) { - if (SvEvent::DEBUGMODE || !sa.isSuspicious()) { - if (sa.getSecondarySupport() > 0 || - (sa.getSupport() > 0 && sa.getMateSupport() > 0)) { - searchSa(j, i, sa, true, mref); - } else { - searchSa(j, i, sa, false, mref); + void + AnnotationProcessor::searchMatches(vector> &mref) { + for (auto j = 0; j < 85; ++j) { + for (auto i = 0u; i < tumorResults[j].size(); ++i) { + for (const auto &sa : tumorResults[j][i].getSuppAlignments()) { + if (SvEvent::DEBUGMODE || !sa.isSuspicious()) { + if (sa.getSecondarySupport() > 0 || + (sa.getSupport() > 0 && sa.getMateSupport() > 0)) { + searchSa(j, i, sa, true, mref); + } else { + searchSa(j, i, sa, false, mref); + } } } } } } -} -void -AnnotationProcessor::searchSa(int chrIndex, int dbIndex, - const SuppAlignmentAnno &sa, bool doubleSupportSa, - vector> &mref) { - if (sa.getSupport() + sa.getSecondarySupport() + sa.getMateSupport() < 3) { - return; - } - if (sa.getChrIndex() == 1001) { - if (createUnknownMatchSvPreCheck(sa, doubleSupportSa)) { - createUnknownMatchSv(tumorResults[chrIndex][dbIndex], sa, mref, - doubleSupportSa); + void + AnnotationProcessor::searchSa(int chrIndex, int dbIndex, + const SuppAlignmentAnno &sa, bool doubleSupportSa, + vector> &mref) { + if (sa.getSupport() + sa.getSecondarySupport() + sa.getMateSupport() < 3) { + return; } - return; - } - auto saChrIndex = GlobalAppConfig::getInstance().getChrConverter(). - indexConverter[sa.getChrIndex()]; - if (saChrIndex < 0) { - return; - } - auto fuzziness = 3 * SuppAlignmentAnno::DEFAULTREADLENGTH; - vector::iterator>> dbHits{}; - - if (!tumorResults[saChrIndex].empty()) { - auto itStart = lower_bound(tumorResults[saChrIndex].begin(), - tumorResults[saChrIndex].end(), sa); - if (itStart == tumorResults[saChrIndex].end()) { - --itStart; + if (sa.getChrIndex() == 1001) { + if (createUnknownMatchSvPreCheck(sa, doubleSupportSa)) { + createUnknownMatchSv(tumorResults[chrIndex][dbIndex], sa, mref, + doubleSupportSa); + } + return; } - if (itStart != tumorResults[saChrIndex].begin() && - !itStart->closeToSupp(sa, fuzziness)) { - --itStart; - if (!itStart->closeToSupp(sa, fuzziness)) { - if (createUnknownMatchSvPreCheck(sa, doubleSupportSa)) { + auto saChrIndex = GlobalAppConfig::getInstance().getChrConverter(). + indexConverter[sa.getChrIndex()]; + if (saChrIndex < 0) { + return; + } + auto fuzziness = 3 * SuppAlignmentAnno::DEFAULTREADLENGTH; + vector::iterator>> dbHits{}; - createUnknownMatchSv(tumorResults[chrIndex][dbIndex], sa, - mref, doubleSupportSa); - } - return; + if (!tumorResults[saChrIndex].empty()) { + auto itStart = lower_bound(tumorResults[saChrIndex].begin(), + tumorResults[saChrIndex].end(), sa); + if (itStart == tumorResults[saChrIndex].end()) { + --itStart; } - } - auto it = itStart; - while (it != tumorResults[saChrIndex].begin()) { - auto distance = it->distanceToSupp(sa); - if (distance <= fuzziness) { - dbHits.emplace_back(distance, it); - --it; - } else { - break; + if (itStart != tumorResults[saChrIndex].begin() && + !itStart->closeToSupp(sa, fuzziness)) { + --itStart; + if (!itStart->closeToSupp(sa, fuzziness)) { + if (createUnknownMatchSvPreCheck(sa, doubleSupportSa)) { + + createUnknownMatchSv(tumorResults[chrIndex][dbIndex], sa, + mref, doubleSupportSa); + } + return; + } } - } - if (itStart != tumorResults[saChrIndex].end()) { - auto it = next(itStart); - while (it != tumorResults[saChrIndex].end()) { + auto it = itStart; + while (it != tumorResults[saChrIndex].begin()) { auto distance = it->distanceToSupp(sa); if (distance <= fuzziness) { dbHits.emplace_back(distance, it); - ++it; + --it; } else { break; } } - } - sort(dbHits.begin(), dbHits.end()); - } - if (dbHits.empty()) { - if (createUnknownMatchSvPreCheck(sa, doubleSupportSa)) { - createUnknownMatchSv(tumorResults[chrIndex][dbIndex], sa, mref, - doubleSupportSa); - } - return; - } else { - auto createdMatch = false; - for (auto &res : dbHits) { - for (const auto &saMatch : res.second->getSuppAlignments()) { - if (tumorResults[chrIndex][dbIndex].closeToSupp(saMatch, - fuzziness)) { - if (createDoubleMatchSvPreCheck(saMatch)) { - createDoubleMatchSv(tumorResults[chrIndex][dbIndex], - *res.second, sa, saMatch, true, - mref); - createdMatch = true; + if (itStart != tumorResults[saChrIndex].end()) { + auto it = next(itStart); + while (it != tumorResults[saChrIndex].end()) { + auto distance = it->distanceToSupp(sa); + if (distance <= fuzziness) { + dbHits.emplace_back(distance, it); + ++it; + } else { + break; } } } + sort(dbHits.begin(), dbHits.end()); } - if (!createdMatch) { - auto res = dbHits[0]; - for (const auto &saMatch : res.second->getSuppAlignments()) { - if (tumorResults[chrIndex][dbIndex].closeToSupp( - saMatch, fuzziness * 3)) { - if (createDoubleMatchSvPreCheck(saMatch)) { - createDoubleMatchSv(tumorResults[chrIndex][dbIndex], - *res.second, sa, saMatch, true, - mref); - return; + if (dbHits.empty()) { + if (createUnknownMatchSvPreCheck(sa, doubleSupportSa)) { + createUnknownMatchSv(tumorResults[chrIndex][dbIndex], sa, mref, + doubleSupportSa); + } + return; + } else { + auto createdMatch = false; + for (auto &res : dbHits) { + for (const auto &saMatch : res.second->getSuppAlignments()) { + if (tumorResults[chrIndex][dbIndex].closeToSupp(saMatch, + fuzziness)) { + if (createDoubleMatchSvPreCheck(saMatch)) { + createDoubleMatchSv(tumorResults[chrIndex][dbIndex], + *res.second, sa, saMatch, true, + mref); + createdMatch = true; + } + } + } + } + if (!createdMatch) { + auto res = dbHits[0]; + for (const auto &saMatch : res.second->getSuppAlignments()) { + if (tumorResults[chrIndex][dbIndex].closeToSupp( + saMatch, fuzziness * 3)) { + if (createDoubleMatchSvPreCheck(saMatch)) { + createDoubleMatchSv(tumorResults[chrIndex][dbIndex], + *res.second, sa, saMatch, true, + mref); + return; + } } } + createUnmatchedSaSv(tumorResults[chrIndex][dbIndex], *res.second, + sa, mref); } - createUnmatchedSaSv(tumorResults[chrIndex][dbIndex], *res.second, - sa, mref); } } -} -void -AnnotationProcessor::createDoubleMatchSv(BreakpointReduced &sourceBp, - BreakpointReduced &targetBp, - const SuppAlignmentAnno &sa, - const SuppAlignmentAnno &saMatch, - bool checkOrder, - vector> &mref) { - if (checkOrder) { + void + AnnotationProcessor::createDoubleMatchSv(BreakpointReduced &sourceBp, + BreakpointReduced &targetBp, + const SuppAlignmentAnno &sa, + const SuppAlignmentAnno &saMatch, + bool checkOrder, + vector> &mref) { + if (checkOrder) { + if (sourceBp.getMrefHits().getNumConsevativeHits() == -1) { + auto germlineInfo = searchGermlineHitsNew( + sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD); + sourceBp.setGermlineInfo(germlineInfo); + auto mrefInfo = searchMrefHitsNew( + sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD, mref); + sourceBp.setMrefHits(mrefInfo); + } + if (targetBp.getMrefHits().getNumConsevativeHits() == -1) { + auto germlineInfo = searchGermlineHitsNew( + targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD); + targetBp.setGermlineInfo(germlineInfo); + auto mrefInfo = searchMrefHitsNew( + targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD, mref); + targetBp.setMrefHits(mrefInfo); + } + if (targetBp.fullSmaller(sourceBp)) { + createDoubleMatchSv(targetBp, sourceBp, saMatch, sa, false, mref); + } + } + visitedLineIndices.push_back(sourceBp.getLineIndex()); + visitedLineIndices.push_back(targetBp.getLineIndex()); + filteredResults.emplace_back(sourceBp, targetBp, sa, saMatch, overhangs); + checkSvQuality(); + } + bool + AnnotationProcessor::createDoubleMatchSvPreCheck( + const SuppAlignmentAnno &saMatch) { + if (SvEvent::DEBUGMODE || !saMatch.isSuspicious()) { + return true; + } + return false; + } + void + AnnotationProcessor::createUnmatchedSaSv(BreakpointReduced &sourceBp, + BreakpointReduced &targetBp, + const SuppAlignmentAnno &sa, + vector> &mref) { if (sourceBp.getMrefHits().getNumConsevativeHits() == -1) { auto germlineInfo = searchGermlineHitsNew( sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, @@ -428,174 +468,112 @@ AnnotationProcessor::createDoubleMatchSv(BreakpointReduced &sourceBp, SvEvent::GERMLINEOFFSETTHRESHOLD, mref); sourceBp.setMrefHits(mrefInfo); } - if (targetBp.getMrefHits().getNumConsevativeHits() == -1) { - auto germlineInfo = searchGermlineHitsNew( - targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD); - targetBp.setGermlineInfo(germlineInfo); - auto mrefInfo = searchMrefHitsNew( - targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD, mref); - targetBp.setMrefHits(mrefInfo); - } - if (targetBp.fullSmaller(sourceBp)) { - createDoubleMatchSv(targetBp, sourceBp, saMatch, sa, false, mref); - } + targetBp.addDummySa(sa, sourceBp); + auto germlineInfo = searchGermlineHitsNew( + targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD); + auto mrefHits = + searchMrefHitsNew(targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD, mref); + targetBp.setMrefHits(mrefHits); + targetBp.setGermlineInfo(germlineInfo); + visitedLineIndices.push_back(sourceBp.getLineIndex()); + visitedLineIndices.push_back(targetBp.getLineIndex()); + filteredResults.emplace_back(sourceBp, targetBp, sa, overhangs, + targetBp.getDummySa()); + checkSvQuality(); + targetBp.removeMarkedFuzzies(); } - visitedLineIndices.push_back(sourceBp.getLineIndex()); - visitedLineIndices.push_back(targetBp.getLineIndex()); - filteredResults.emplace_back(sourceBp, targetBp, sa, saMatch, overhangs); - checkSvQuality(); -} -bool -AnnotationProcessor::createDoubleMatchSvPreCheck( - const SuppAlignmentAnno &saMatch) { - if (SvEvent::DEBUGMODE || !saMatch.isSuspicious()) { - return true; + bool + AnnotationProcessor::createUnknownMatchSvPreCheck(const SuppAlignmentAnno &sa, + bool doubleSupportSa) { + if (SvEvent::DEBUGMODE || !sa.isSemiSuspicious()) { + if (doubleSupportSa || + (!sa.isFuzzy() && + (sa.getSupport() > 0 || sa.getSecondarySupport() > 0))) { + return true; + } + } + return false; } - return false; -} -void -AnnotationProcessor::createUnmatchedSaSv(BreakpointReduced &sourceBp, - BreakpointReduced &targetBp, - const SuppAlignmentAnno &sa, - vector> &mref) { - if (sourceBp.getMrefHits().getNumConsevativeHits() == -1) { + void + AnnotationProcessor::createUnknownMatchSv(BreakpointReduced &sourceBp, + const SuppAlignmentAnno &sa, + vector> &mref, + bool doubleSupportSa) { auto germlineInfo = searchGermlineHitsNew( sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, SvEvent::GERMLINEOFFSETTHRESHOLD); sourceBp.setGermlineInfo(germlineInfo); - auto mrefInfo = searchMrefHitsNew( - sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD, mref); + auto mrefInfo = + searchMrefHitsNew(sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD, mref); sourceBp.setMrefHits(mrefInfo); - } - targetBp.addDummySa(sa, sourceBp); - auto germlineInfo = searchGermlineHitsNew( - targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD); - auto mrefHits = - searchMrefHitsNew(targetBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD, mref); - targetBp.setMrefHits(mrefHits); - targetBp.setGermlineInfo(germlineInfo); - visitedLineIndices.push_back(sourceBp.getLineIndex()); - visitedLineIndices.push_back(targetBp.getLineIndex()); - filteredResults.emplace_back(sourceBp, targetBp, sa, overhangs, - targetBp.getDummySa()); - checkSvQuality(); - targetBp.removeMarkedFuzzies(); -} -bool -AnnotationProcessor::createUnknownMatchSvPreCheck(const SuppAlignmentAnno &sa, - bool doubleSupportSa) { - if (SvEvent::DEBUGMODE || !sa.isSemiSuspicious()) { - if (doubleSupportSa || - (!sa.isFuzzy() && - (sa.getSupport() > 0 || sa.getSecondarySupport() > 0))) { - return true; - } - } - return false; -} -void -AnnotationProcessor::createUnknownMatchSv(BreakpointReduced &sourceBp, - const SuppAlignmentAnno &sa, - vector> &mref, - bool doubleSupportSa) { - auto germlineInfo = searchGermlineHitsNew( - sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD); - sourceBp.setGermlineInfo(germlineInfo); - auto mrefInfo = - searchMrefHitsNew(sourceBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD, mref); - sourceBp.setMrefHits(mrefInfo); - BreakpointReduced dummyBp{sa, sourceBp, false}; - auto dummyGermline = - searchGermlineHitsNew(dummyBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD * 3); - auto dummyMref = - searchMrefHitsNew(dummyBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD * 3, mref); - if (sa.isFuzzy() && sa.getExtendedPos() - sa.getPos() > - 3 * SvEvent::GERMLINEOFFSETTHRESHOLD) { - BreakpointReduced dummyBp2{sa, sourceBp, true}; - auto dummyGermline2 = searchGermlineHitsNew( - dummyBp2, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD * 3); - auto dummyMref2 = searchMrefHitsNew( - dummyBp2, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD * 3, mref); - if (dummyMref2.getNumConsevativeHits() > - dummyMref.getNumConsevativeHits()) { - dummyMref = dummyMref2; - dummyGermline = dummyGermline2; + BreakpointReduced dummyBp{sa, sourceBp, false}; + auto dummyGermline = + searchGermlineHitsNew(dummyBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD * 3); + auto dummyMref = + searchMrefHitsNew(dummyBp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD * 3, mref); + if (sa.isFuzzy() && sa.getExtendedPos() - sa.getPos() > + 3 * SvEvent::GERMLINEOFFSETTHRESHOLD) { + BreakpointReduced dummyBp2{sa, sourceBp, true}; + auto dummyGermline2 = searchGermlineHitsNew( + dummyBp2, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD * 3); + auto dummyMref2 = searchMrefHitsNew( + dummyBp2, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD * 3, mref); + if (dummyMref2.getNumConsevativeHits() > + dummyMref.getNumConsevativeHits()) { + dummyMref = dummyMref2; + dummyGermline = dummyGermline2; + } } + visitedLineIndices.push_back(sourceBp.getLineIndex()); + filteredResults.emplace_back(sourceBp, sa, dummyGermline, dummyMref, + overhangs, dummyBp.getDummySa()); + checkSvQuality(); } - visitedLineIndices.push_back(sourceBp.getLineIndex()); - filteredResults.emplace_back(sourceBp, sa, dummyGermline, dummyMref, - overhangs, dummyBp.getDummySa()); - checkSvQuality(); -} -void -AnnotationProcessor::checkSvQuality() { - auto key = filteredResults.back().getKey(); - if (!key.empty()) { - if (filteredResultKeys.count(key) == 0) { - filteredResultKeys.insert(key); - return; + void + AnnotationProcessor::checkSvQuality() { + auto key = filteredResults.back().getKey(); + if (!key.empty()) { + if (filteredResultKeys.count(key) == 0) { + filteredResultKeys.insert(key); + return; + } } + filteredResults.pop_back(); } - filteredResults.pop_back(); -} -MrefMatch -AnnotationProcessor::searchMrefHitsNew(const BreakpointReduced &bpIn, - int distanceThreshold, - int conservativeDistanceThreshold, - vector> &mref) { - auto convertedChrIndex = GlobalAppConfig::getInstance().getChrConverter(). - indexConverter[bpIn.getChrIndex()]; - vector suppMatches{}; - if (convertedChrIndex < 0) { - return MrefMatch{0, 0, 10000, suppMatches}; - } - auto itStart = lower_bound(mref[convertedChrIndex].begin(), - mref[convertedChrIndex].end(), bpIn); - if (itStart == mref[convertedChrIndex].end()) { - return MrefMatch{0, 0, 10000, suppMatches}; - } - if (itStart != mref[convertedChrIndex].begin() && - !(itStart->distanceTo(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) && - prev(itStart)->distanceTo(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) { - --itStart; - } - auto it = itStart; - - vector::iterator> dbHits{}; - vector::iterator> dbHitsConservative{}; - while (true) { - auto tmpDistance = it->distanceTo(bpIn); - if (tmpDistance < SvEvent::GERMLINEOFFSETTHRESHOLD) { - dbHitsConservative.push_back(it); + MrefMatch + AnnotationProcessor::searchMrefHitsNew(const BreakpointReduced &bpIn, + int distanceThreshold, + int conservativeDistanceThreshold, + vector> &mref) { + auto convertedChrIndex = GlobalAppConfig::getInstance().getChrConverter(). + indexConverter[bpIn.getChrIndex()]; + vector suppMatches{}; + if (convertedChrIndex < 0) { + return MrefMatch{0, 0, 10000, suppMatches}; } - if (tmpDistance < distanceThreshold) { - dbHits.push_back(it); - } else { - break; + auto itStart = lower_bound(mref[convertedChrIndex].begin(), + mref[convertedChrIndex].end(), bpIn); + if (itStart == mref[convertedChrIndex].end()) { + return MrefMatch{0, 0, 10000, suppMatches}; } - if (it == mref[convertedChrIndex].begin()) { - break; + if (itStart != mref[convertedChrIndex].begin() && + !(itStart->distanceTo(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) && + prev(itStart)->distanceTo(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) { + --itStart; } - --it; - } - if (itStart != mref[convertedChrIndex].end()) { - it = next(itStart); + auto it = itStart; + + vector::iterator> dbHits{}; + vector::iterator> dbHitsConservative{}; while (true) { - if (it == mref[convertedChrIndex].end()) { - break; - } auto tmpDistance = it->distanceTo(bpIn); if (tmpDistance < SvEvent::GERMLINEOFFSETTHRESHOLD) { dbHitsConservative.push_back(it); @@ -605,139 +583,136 @@ AnnotationProcessor::searchMrefHitsNew(const BreakpointReduced &bpIn, } else { break; } - ++it; + if (it == mref[convertedChrIndex].begin()) { + break; + } + --it; } - } - if (dbHits.empty()) { - return MrefMatch{0, 0, 0, suppMatches}; - } - short score{0}; - auto offset = 0; - for (auto res : dbHits) { - auto saMatch = false; - for (const auto &saRef : res->getSuppAlignments()) { - for (const auto &sa : bpIn.getSuppAlignments()) { - if (saRef.saCloseness(sa, SuppAlignmentAnno::DEFAULTREADLENGTH / - 2)) { - saMatch = true; - auto previouslyRecorded = false; - for (auto &saTmp : suppMatches) { - if (saRef.saCloseness( - saTmp, - SuppAlignmentAnno::DEFAULTREADLENGTH / 2)) { - previouslyRecorded = true; - if (saTmp.isFuzzy()) { - if (saRef.isFuzzy()) { - saTmp.extendSuppAlignment( - saRef.getPos(), saRef.getExtendedPos()); - } else { - saTmp.removeFuzziness(saRef); + if (itStart != mref[convertedChrIndex].end()) { + it = next(itStart); + while (true) { + if (it == mref[convertedChrIndex].end()) { + break; + } + auto tmpDistance = it->distanceTo(bpIn); + if (tmpDistance < SvEvent::GERMLINEOFFSETTHRESHOLD) { + dbHitsConservative.push_back(it); + } + if (tmpDistance < distanceThreshold) { + dbHits.push_back(it); + } else { + break; + } + ++it; + } + } + if (dbHits.empty()) { + return MrefMatch{0, 0, 0, suppMatches}; + } + short score{0}; + auto offset = 0; + for (auto res : dbHits) { + auto saMatch = false; + for (const auto &saRef : res->getSuppAlignments()) { + for (const auto &sa : bpIn.getSuppAlignments()) { + if (saRef.saCloseness(sa, SuppAlignmentAnno::DEFAULTREADLENGTH / + 2)) { + saMatch = true; + auto previouslyRecorded = false; + for (auto &saTmp : suppMatches) { + if (saRef.saCloseness( + saTmp, + SuppAlignmentAnno::DEFAULTREADLENGTH / 2)) { + previouslyRecorded = true; + if (saTmp.isFuzzy()) { + if (saRef.isFuzzy()) { + saTmp.extendSuppAlignment( + saRef.getPos(), saRef.getExtendedPos()); + } else { + saTmp.removeFuzziness(saRef); + } + } + if (saRef.getSupport() > saTmp.getSupport()) { + saTmp.setSupport(saRef.getSupport()); + } + if (saRef.getSecondarySupport() > + saTmp.getSecondarySupport()) { + saTmp.setSecondarySupport( + saRef.getSecondarySupport()); + } + if (saTmp.getSecondarySupport() < + saTmp.getSupport()) { + saTmp.setSecondarySupport(saTmp.getSupport()); } - } - if (saRef.getSupport() > saTmp.getSupport()) { - saTmp.setSupport(saRef.getSupport()); - } - if (saRef.getSecondarySupport() > - saTmp.getSecondarySupport()) { - saTmp.setSecondarySupport( - saRef.getSecondarySupport()); - } - if (saTmp.getSecondarySupport() < - saTmp.getSupport()) { - saTmp.setSecondarySupport(saTmp.getSupport()); } } - } - if (!previouslyRecorded) { - suppMatches.push_back(saRef); + if (!previouslyRecorded) { + suppMatches.push_back(saRef); + } } } } - } - if (saMatch) { - auto tmpScore = res->getNumHits(); - if (tmpScore > score) { - score = tmpScore; - offset = res->distanceTo(bpIn); + if (saMatch) { + auto tmpScore = res->getNumHits(); + if (tmpScore > score) { + score = tmpScore; + offset = res->distanceTo(bpIn); + } } } - } - short conservativeScore{0}; - for (const auto res : dbHitsConservative) { - auto tmpScore = res->getNumHits(); - if (tmpScore < SvEvent::RELAXEDBPFREQTHRESHOLD) { - auto sas = res->getSuppAlignments(); - if (sas.size() == 1) { - if ((sas[0].getSupport() + 0.0) / tmpScore > 0.8) { - continue; + short conservativeScore{0}; + for (const auto res : dbHitsConservative) { + auto tmpScore = res->getNumHits(); + if (tmpScore < SvEvent::RELAXEDBPFREQTHRESHOLD) { + auto sas = res->getSuppAlignments(); + if (sas.size() == 1) { + if ((sas[0].getSupport() + 0.0) / tmpScore > 0.8) { + continue; + } } } + if (tmpScore > conservativeScore) { + conservativeScore = tmpScore; + } } - if (tmpScore > conservativeScore) { - conservativeScore = tmpScore; - } + return MrefMatch{max(score, conservativeScore), conservativeScore, offset, + suppMatches}; } - return MrefMatch{max(score, conservativeScore), conservativeScore, offset, - suppMatches}; -} -GermlineMatch -AnnotationProcessor::searchGermlineHitsNew(const BreakpointReduced &bpIn, - int distanceThreshold, - int conservativeDistanceThreshold) { - GermlineMatch dummyMatchTrue{1.0, 1.0, - vector>{}}; - GermlineMatch dummyMatchFalse{0.0, 0.0, - vector>{}}; - if (NOCONTROLMODE) { - return dummyMatchTrue; - } - auto convertedChrIndex = GlobalAppConfig::getInstance().getChrConverter(). - indexConverter[bpIn.getChrIndex()]; - if (convertedChrIndex < 0) { - return dummyMatchFalse; - } - if (controlResults[convertedChrIndex].empty()) { - return dummyMatchFalse; - } - auto itStart = lower_bound(controlResults[convertedChrIndex].begin(), - controlResults[convertedChrIndex].end(), bpIn); - if (itStart == controlResults[convertedChrIndex].end()) { - return dummyMatchFalse; - } - if (itStart != controlResults[convertedChrIndex].cbegin() && - !(itStart->distanceToBp(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) && - prev(itStart)->distanceToBp(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) { - --itStart; - } - auto it = itStart; - vector::iterator> dbHits{}; - vector::iterator> dbHitsConservative{}; - - while (true) { - auto tmpDistance = it->distanceToBp(bpIn); - if (tmpDistance < 0) { - break; + GermlineMatch + AnnotationProcessor::searchGermlineHitsNew(const BreakpointReduced &bpIn, + int distanceThreshold, + int conservativeDistanceThreshold) { + GermlineMatch dummyMatchTrue{1.0, 1.0, + vector>{}}; + GermlineMatch dummyMatchFalse{0.0, 0.0, + vector>{}}; + if (NOCONTROLMODE) { + return dummyMatchTrue; } - if (tmpDistance < conservativeDistanceThreshold) { - dbHitsConservative.push_back(it); + auto convertedChrIndex = GlobalAppConfig::getInstance().getChrConverter(). + indexConverter[bpIn.getChrIndex()]; + if (convertedChrIndex < 0) { + return dummyMatchFalse; } - if (tmpDistance < distanceThreshold) { - dbHits.push_back(it); - } else { - break; + if (controlResults[convertedChrIndex].empty()) { + return dummyMatchFalse; } - if (it == controlResults[convertedChrIndex].begin()) { - break; + auto itStart = lower_bound(controlResults[convertedChrIndex].begin(), + controlResults[convertedChrIndex].end(), bpIn); + if (itStart == controlResults[convertedChrIndex].end()) { + return dummyMatchFalse; } - --it; - } - if (itStart != controlResults[convertedChrIndex].end()) { - it = next(itStart); + if (itStart != controlResults[convertedChrIndex].cbegin() && + !(itStart->distanceToBp(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) && + prev(itStart)->distanceToBp(bpIn) < SvEvent::GERMLINEOFFSETTHRESHOLD) { + --itStart; + } + auto it = itStart; + vector::iterator> dbHits{}; + vector::iterator> dbHitsConservative{}; + while (true) { - if (it == controlResults[convertedChrIndex].end()) { - break; - } auto tmpDistance = it->distanceToBp(bpIn); if (tmpDistance < 0) { break; @@ -750,362 +725,370 @@ AnnotationProcessor::searchGermlineHitsNew(const BreakpointReduced &bpIn, } else { break; } - ++it; + if (it == controlResults[convertedChrIndex].begin()) { + break; + } + --it; } - } - if (dbHits.empty()) { - return dummyMatchFalse; - } - auto conservativeClonality = 0.0; - if (!dbHitsConservative.empty()) { - auto maxSupport = 0.0; - for (const auto res : dbHitsConservative) { - auto mateSupSa = 0; - for (const auto sa : res->getSuppAlignments()) { - if (sa.getMateSupport() > mateSupSa) { - mateSupSa = sa.getMateSupport(); + if (itStart != controlResults[convertedChrIndex].end()) { + it = next(itStart); + while (true) { + if (it == controlResults[convertedChrIndex].end()) { + break; } + auto tmpDistance = it->distanceToBp(bpIn); + if (tmpDistance < 0) { + break; + } + if (tmpDistance < conservativeDistanceThreshold) { + dbHitsConservative.push_back(it); + } + if (tmpDistance < distanceThreshold) { + dbHits.push_back(it); + } else { + break; + } + ++it; } - if (res->getMateSupport() > mateSupSa) { - mateSupSa = res->getMateSupport(); - } - auto support = - 0.0 + res->getPairedBreaksSoft() + res->getBreaksShortIndel() + - res->getPairedBreaksHard() + res->getUnpairedBreaksSoft() + - res->getUnpairedBreaksHard() + mateSupSa; - if (support > maxSupport) { - maxSupport = support; - if (support + res->getNormalSpans() > 0) { - conservativeClonality = - support / (support + res->getNormalSpans()); + } + if (dbHits.empty()) { + return dummyMatchFalse; + } + auto conservativeClonality = 0.0; + if (!dbHitsConservative.empty()) { + auto maxSupport = 0.0; + for (const auto res : dbHitsConservative) { + auto mateSupSa = 0; + for (const auto sa : res->getSuppAlignments()) { + if (sa.getMateSupport() > mateSupSa) { + mateSupSa = sa.getMateSupport(); + } + } + if (res->getMateSupport() > mateSupSa) { + mateSupSa = res->getMateSupport(); + } + auto support = + 0.0 + res->getPairedBreaksSoft() + res->getBreaksShortIndel() + + res->getPairedBreaksHard() + res->getUnpairedBreaksSoft() + + res->getUnpairedBreaksHard() + mateSupSa; + if (support > maxSupport) { + maxSupport = support; + if (support + res->getNormalSpans() > 0) { + conservativeClonality = + support / (support + res->getNormalSpans()); + } } } } - } - auto clonality = conservativeClonality; - vector> suppMatches{}; - for (auto res : dbHits) { - auto breakSupportSoft = - res->getPairedBreaksSoft() + res->getUnpairedBreaksSoft(); - auto breakSupportHard = - res->getPairedBreaksHard() + res->getUnpairedBreaksHard(); - for (const auto &saRef : res->getSuppAlignments()) { - for (const auto &sa : bpIn.getSuppAlignments()) { - if (saRef.saCloseness(sa, SuppAlignmentAnno::DEFAULTREADLENGTH / - 2)) { - auto previouslyRecorded = false; - for (auto &saTmp : suppMatches) { - if (saRef.saClosenessDirectional( - saTmp.first, - SuppAlignmentAnno::DEFAULTREADLENGTH / 2)) { - previouslyRecorded = true; - if (saTmp.first.isFuzzy()) { - if (saRef.isFuzzy()) { - saTmp.first.extendSuppAlignment( - saRef.getPos(), saRef.getExtendedPos()); - } else { - saTmp.first.removeFuzziness(saRef); + auto clonality = conservativeClonality; + vector> suppMatches{}; + for (auto res : dbHits) { + auto breakSupportSoft = + res->getPairedBreaksSoft() + res->getUnpairedBreaksSoft(); + auto breakSupportHard = + res->getPairedBreaksHard() + res->getUnpairedBreaksHard(); + for (const auto &saRef : res->getSuppAlignments()) { + for (const auto &sa : bpIn.getSuppAlignments()) { + if (saRef.saCloseness(sa, SuppAlignmentAnno::DEFAULTREADLENGTH / + 2)) { + auto previouslyRecorded = false; + for (auto &saTmp : suppMatches) { + if (saRef.saClosenessDirectional( + saTmp.first, + SuppAlignmentAnno::DEFAULTREADLENGTH / 2)) { + previouslyRecorded = true; + if (saTmp.first.isFuzzy()) { + if (saRef.isFuzzy()) { + saTmp.first.extendSuppAlignment( + saRef.getPos(), saRef.getExtendedPos()); + } else { + saTmp.first.removeFuzziness(saRef); + } } + if (saRef.getSupport() > saTmp.first.getSupport()) { + saTmp.first.setSupport(saRef.getSupport()); + } + if (saRef.getSecondarySupport() > + saTmp.first.getSecondarySupport()) { + saTmp.first.setSecondarySupport( + saRef.getSecondarySupport()); + } + if (saRef.getMateSupport() > + saTmp.first.getMateSupport()) { + saTmp.first.setMateSupport( + saRef.getMateSupport()); + saTmp.first.setExpectedDiscordants( + saRef.getExpectedDiscordants()); + } + auto currentSoftSupport = + max(saTmp.first.getSupport(), breakSupportSoft); + auto currentHardSupport = + max(saTmp.first.getSecondarySupport(), + breakSupportHard); + auto breakSupport = + currentSoftSupport + currentHardSupport + 0.0; + auto currentClonality = + (breakSupport + saTmp.first.getMateSupport()) / + (breakSupport + saTmp.first.getMateSupport() + + res->getNormalSpans()); + saTmp.second = max(currentClonality, saTmp.second); } - if (saRef.getSupport() > saTmp.first.getSupport()) { - saTmp.first.setSupport(saRef.getSupport()); - } - if (saRef.getSecondarySupport() > - saTmp.first.getSecondarySupport()) { - saTmp.first.setSecondarySupport( - saRef.getSecondarySupport()); - } - if (saRef.getMateSupport() > - saTmp.first.getMateSupport()) { - saTmp.first.setMateSupport( - saRef.getMateSupport()); - saTmp.first.setExpectedDiscordants( - saRef.getExpectedDiscordants()); - } + } + if (!previouslyRecorded) { auto currentSoftSupport = - max(saTmp.first.getSupport(), breakSupportSoft); + max(saRef.getSupport(), breakSupportSoft); auto currentHardSupport = - max(saTmp.first.getSecondarySupport(), - breakSupportHard); + max(saRef.getSecondarySupport(), breakSupportHard); auto breakSupport = currentSoftSupport + currentHardSupport + 0.0; auto currentClonality = - (breakSupport + saTmp.first.getMateSupport()) / - (breakSupport + saTmp.first.getMateSupport() + + (breakSupport + saRef.getMateSupport()) / + (breakSupport + saRef.getMateSupport() + res->getNormalSpans()); - saTmp.second = max(currentClonality, saTmp.second); + suppMatches.push_back({saRef, currentClonality}); } } - if (!previouslyRecorded) { - auto currentSoftSupport = - max(saRef.getSupport(), breakSupportSoft); - auto currentHardSupport = - max(saRef.getSecondarySupport(), breakSupportHard); - auto breakSupport = - currentSoftSupport + currentHardSupport + 0.0; - auto currentClonality = - (breakSupport + saRef.getMateSupport()) / - (breakSupport + saRef.getMateSupport() + - res->getNormalSpans()); - suppMatches.push_back({saRef, currentClonality}); - } } } } + return GermlineMatch{clonality, conservativeClonality, suppMatches}; } - return GermlineMatch{clonality, conservativeClonality, suppMatches}; -} -bool -AnnotationProcessor::applyMassiveInversionFiltering(bool stricterMode, - bool controlCheckMode) { - auto deletionCandidateCount = 0; - auto totalCount = 0; - for (const auto &sv : filteredResults) { - if (sv.isToRemove()) { - continue; - } - if (sv.getContaminationCandidate() > 0) { - continue; - } - if (sv.getSuspicious() == 0 && sv.getEventScore() > 2) { - if (sv.isInverted() || sv.isSemiSuspicious()) { - ++deletionCandidateCount; - } else if (stricterMode && sv.getEvidenceLevel2() == 0) { - ++deletionCandidateCount; - } - ++totalCount; - } - } - auto totalCountThreshold = 300; - if (stricterMode || controlCheckMode) { - totalCountThreshold = 200; - } - auto invRatio = (deletionCandidateCount + 0.0) / totalCount; - if (invRatio > 0.7 && totalCount > totalCountThreshold) { - for (auto &sv : filteredResults) { + bool + AnnotationProcessor::applyMassiveInversionFiltering(bool stricterMode, + bool controlCheckMode) { + auto deletionCandidateCount = 0; + auto totalCount = 0; + for (const auto &sv : filteredResults) { if (sv.isToRemove()) { continue; } if (sv.getContaminationCandidate() > 0) { continue; } - if (sv.getSuspicious() != 0) { - continue; + if (sv.getSuspicious() == 0 && sv.getEventScore() > 2) { + if (sv.isInverted() || sv.isSemiSuspicious()) { + ++deletionCandidateCount; + } else if (stricterMode && sv.getEvidenceLevel2() == 0) { + ++deletionCandidateCount; + } + ++totalCount; } - if (sv.isDistant()) { - if (sv.getSelectedSa1().isProperPairErrorProne() && - sv.getSelectedSa1().getMateSupport() < 6) { - sv.setToRemove(true); + } + auto totalCountThreshold = 300; + if (stricterMode || controlCheckMode) { + totalCountThreshold = 200; + } + auto invRatio = (deletionCandidateCount + 0.0) / totalCount; + if (invRatio > 0.7 && totalCount > totalCountThreshold) { + for (auto &sv : filteredResults) { + if (sv.isToRemove()) { continue; } - if (sv.getSelectedSa2().isProperPairErrorProne() && - sv.getSelectedSa2().getMateSupport() < 6) { - sv.setToRemove(true); + if (sv.getContaminationCandidate() > 0) { continue; } - if (sv.isSemiSuspicious()) { - if (sv.getEventScore() < 3) { + if (sv.getSuspicious() != 0) { + continue; + } + if (sv.isDistant()) { + if (sv.getSelectedSa1().isProperPairErrorProne() && + sv.getSelectedSa1().getMateSupport() < 6) { sv.setToRemove(true); continue; } - if (sv.getSelectedSa1().isSemiSuspicious() && - sv.getSelectedSa2().isSemiSuspicious()) { + if (sv.getSelectedSa2().isProperPairErrorProne() && + sv.getSelectedSa2().getMateSupport() < 6) { sv.setToRemove(true); continue; } - if (sv.getEvidenceLevel2() < 3 && - sv.getSelectedSa1().isSemiSuspicious()) { - if (sv.getEvidenceLevel1() < 3 || - sv.getSelectedSa1().getSupport() < 5) { + if (sv.isSemiSuspicious()) { + if (sv.getEventScore() < 3) { sv.setToRemove(true); continue; } - } - if (sv.getEvidenceLevel1() < 3 && - sv.getSelectedSa2().isSemiSuspicious()) { - if (sv.getEvidenceLevel2() < 3 || - sv.getSelectedSa2().getSupport() < 5) { + if (sv.getSelectedSa1().isSemiSuspicious() && + sv.getSelectedSa2().isSemiSuspicious()) { sv.setToRemove(true); continue; } - } - } - } else { - if (sv.getEventScore() == 3 || sv.getEvidenceLevel2() == 0) { - sv.setToRemove(true); - continue; - } - } - if (sv.isInverted()) { - if (stricterMode) { - if (sv.getSelectedSa1().isStrictFuzzyCandidate() || - sv.getSelectedSa2().isStrictFuzzyCandidate()) { - sv.setToRemove(true); - continue; - } - if (sv.isSemiSuspicious()) { - sv.setToRemove(true); - continue; - } - } else { - if (sv.getSelectedSa1().isStrictFuzzyCandidate() && - sv.getSelectedSa2().isStrictFuzzyCandidate()) { - sv.setToRemove(true); - continue; - } - } - if (sv.getEventScore() < 3) { - sv.setToRemove(true); - continue; - } - if (sv.getMateRatio1() < 0.6 && sv.getMateRatio2() < 0.6) { - sv.setToRemove(true); - continue; - } - if (sv.getEventSize() > 0 && sv.getEventSize() < 10000) { - if (stricterMode || sv.getTotalEvidence1() < 5 || - sv.getTotalEvidence2() < 5) { - sv.setToRemove(true); - continue; + if (sv.getEvidenceLevel2() < 3 && + sv.getSelectedSa1().isSemiSuspicious()) { + if (sv.getEvidenceLevel1() < 3 || + sv.getSelectedSa1().getSupport() < 5) { + sv.setToRemove(true); + continue; + } + } + if (sv.getEvidenceLevel1() < 3 && + sv.getSelectedSa2().isSemiSuspicious()) { + if (sv.getEvidenceLevel2() < 3 || + sv.getSelectedSa2().getSupport() < 5) { + sv.setToRemove(true); + continue; + } + } } } else { - if (sv.getTotalEvidence1() < 5 && - sv.getTotalEvidence2() < 5) { + if (sv.getEventScore() == 3 || sv.getEvidenceLevel2() == 0) { sv.setToRemove(true); continue; } } - } - if (stricterMode) { - if (sv.getEvidenceLevel2() == 0) { - if (sv.getSelectedSa1().getSupport() < 10 || - !sv.isOverhang1Compensation()) { - sv.setToRemove(true); - continue; + if (sv.isInverted()) { + if (stricterMode) { + if (sv.getSelectedSa1().isStrictFuzzyCandidate() || + sv.getSelectedSa2().isStrictFuzzyCandidate()) { + sv.setToRemove(true); + continue; + } + if (sv.isSemiSuspicious()) { + sv.setToRemove(true); + continue; + } + } else { + if (sv.getSelectedSa1().isStrictFuzzyCandidate() && + sv.getSelectedSa2().isStrictFuzzyCandidate()) { + sv.setToRemove(true); + continue; + } } - } - if (sv.getEventScore() == 3) { - if (sv.getSelectedSa1().getSupport() < 10) { + if (sv.getEventScore() < 3) { sv.setToRemove(true); continue; } - if (sv.getSelectedSa1().getSecondarySupport() < 5) { + if (sv.getMateRatio1() < 0.6 && sv.getMateRatio2() < 0.6) { sv.setToRemove(true); continue; } - if (sv.isDistant()) { - if (sv.getSelectedSa1().getMateSupport() < 10) { + if (sv.getEventSize() > 0 && sv.getEventSize() < 10000) { + if (stricterMode || sv.getTotalEvidence1() < 5 || + sv.getTotalEvidence2() < 5) { sv.setToRemove(true); continue; } - } - } - if (sv.isDistant()) { - if (sv.getSelectedSa1().isProperPairErrorProne() && - (sv.getSelectedSa2().isProperPairErrorProne() || - sv.getEvidenceLevel2() == 0)) { - if (sv.getSelectedSa1().getSupport() < 5 || - sv.getSelectedSa2().getSupport() < 5) { + } else { + if (sv.getTotalEvidence1() < 5 && + sv.getTotalEvidence2() < 5) { sv.setToRemove(true); continue; } - if (sv.getSelectedSa1().getSecondarySupport() < 3 && - sv.getSelectedSa2().getSecondarySupport() < 3) { + } + } + if (stricterMode) { + if (sv.getEvidenceLevel2() == 0) { + if (sv.getSelectedSa1().getSupport() < 10 || + !sv.isOverhang1Compensation()) { sv.setToRemove(true); continue; } } - if (sv.getSelectedSa1().isSemiSuspicious()) { - if (sv.getEvidenceLevel1() < 3 || - sv.getSelectedSa1().getSupport() < 5) { + if (sv.getEventScore() == 3) { + if (sv.getSelectedSa1().getSupport() < 10) { sv.setToRemove(true); continue; } - } - if (sv.getSelectedSa2().isSemiSuspicious()) { - if (sv.getEvidenceLevel2() < 3 || - sv.getSelectedSa2().getSupport() < 5) { + if (sv.getSelectedSa1().getSecondarySupport() < 5) { sv.setToRemove(true); continue; } + if (sv.isDistant()) { + if (sv.getSelectedSa1().getMateSupport() < 10) { + sv.setToRemove(true); + continue; + } + } } + if (sv.isDistant()) { + if (sv.getSelectedSa1().isProperPairErrorProne() && + (sv.getSelectedSa2().isProperPairErrorProne() || + sv.getEvidenceLevel2() == 0)) { + if (sv.getSelectedSa1().getSupport() < 5 || + sv.getSelectedSa2().getSupport() < 5) { + sv.setToRemove(true); + continue; + } + if (sv.getSelectedSa1().getSecondarySupport() < 3 && + sv.getSelectedSa2().getSecondarySupport() < 3) { + sv.setToRemove(true); + continue; + } + } + if (sv.getSelectedSa1().isSemiSuspicious()) { + if (sv.getEvidenceLevel1() < 3 || + sv.getSelectedSa1().getSupport() < 5) { + sv.setToRemove(true); + continue; + } + } + if (sv.getSelectedSa2().isSemiSuspicious()) { + if (sv.getEvidenceLevel2() < 3 || + sv.getSelectedSa2().getSupport() < 5) { + sv.setToRemove(true); + continue; + } + } - if (sv.getEvidenceLevel2() == 0) { - if (sv.getEvidenceLevel1() < 3 || - sv.getSelectedSa1().getSupport() < 5) { + if (sv.getEvidenceLevel2() == 0) { + if (sv.getEvidenceLevel1() < 3 || + sv.getSelectedSa1().getSupport() < 5) { + sv.setToRemove(true); + continue; + } + } + if (sv.getEvidenceLevel1() < 3 && + sv.getEvidenceLevel2() < 3) { + sv.setToRemove(true); + continue; + } + } else { + if (sv.getEvidenceLevel1() < 2 || + sv.getEvidenceLevel2() < 2) { + sv.setToRemove(true); + continue; + } + if (sv.getTotalEvidence1() < 5 && + sv.getTotalEvidence2() < 5) { sv.setToRemove(true); continue; } } - if (sv.getEvidenceLevel1() < 3 && - sv.getEvidenceLevel2() < 3) { - sv.setToRemove(true); - continue; - } - } else { - if (sv.getEvidenceLevel1() < 2 || - sv.getEvidenceLevel2() < 2) { - sv.setToRemove(true); - continue; - } - if (sv.getTotalEvidence1() < 5 && - sv.getTotalEvidence2() < 5) { - sv.setToRemove(true); - continue; - } - } - if (sv.getTotalEvidence2() < 5) { - auto evidenceLevelThreshold = sv.isDistant() ? 3 : 2; - if (sv.getEvidenceLevel1() < evidenceLevelThreshold) { - sv.setToRemove(true); - continue; + if (sv.getTotalEvidence2() < 5) { + auto evidenceLevelThreshold = sv.isDistant() ? 3 : 2; + if (sv.getEvidenceLevel1() < evidenceLevelThreshold) { + sv.setToRemove(true); + continue; + } } } } + return true; } - return true; - } - return false; -} -bool -AnnotationProcessor::applyPathogenContaminationFiltering() { - auto likelyContaminants = 0; - for (auto &sv : filteredResults) { - if (sv.isToRemove()) { - continue; - } - if (sv.getContaminationCandidate() != 2) { - continue; - } - if (sv.getSuspicious() != 0) { - continue; - } - if (sv.getEventScore() < 3) { - continue; - } - ++likelyContaminants; - if (likelyContaminants > 9) { - break; - } + return false; } - if (likelyContaminants > 9) { - auto cleanedContaminants = 0; + bool + AnnotationProcessor::applyPathogenContaminationFiltering() { + auto likelyContaminants = 0; for (auto &sv : filteredResults) { if (sv.isToRemove()) { continue; } + if (sv.getContaminationCandidate() != 2) { + continue; + } if (sv.getSuspicious() != 0) { continue; } if (sv.getEventScore() < 3) { continue; } - if (sv.getContaminationCandidate() == 2) { - ++cleanedContaminants; + ++likelyContaminants; + if (likelyContaminants > 9) { + break; } } - if (cleanedContaminants > 19) { + if (likelyContaminants > 9) { + auto cleanedContaminants = 0; for (auto &sv : filteredResults) { if (sv.isToRemove()) { continue; @@ -1113,116 +1096,131 @@ AnnotationProcessor::applyPathogenContaminationFiltering() { if (sv.getSuspicious() != 0) { continue; } - if (sv.getEventScore() < 2) { - continue; - } - if (sv.getContaminationCandidate() > 0) { - sv.setEventScore(1); - sv.setEventType(5); - continue; - } - if (sv.getSelectedSa1().getSupport() > 19 && - sv.getSelectedSa1().getSecondarySupport() < 3) { - sv.setEventScore(1); - sv.setEventType(5); + if (sv.getEventScore() < 3) { continue; } - if (sv.getSelectedSa2().getSupport() > 19 && - sv.getSelectedSa2().getSecondarySupport() < 3) { - sv.setEventScore(1); - sv.setEventType(5); - continue; + if (sv.getContaminationCandidate() == 2) { + ++cleanedContaminants; } - if (sv.getOverhang1lengthRatio() > 0.7 || - sv.getOverhang2lengthRatio() > 0.7) { - sv.setEventScore(1); - sv.setEventType(5); - continue; + } + if (cleanedContaminants > 19) { + for (auto &sv : filteredResults) { + if (sv.isToRemove()) { + continue; + } + if (sv.getSuspicious() != 0) { + continue; + } + if (sv.getEventScore() < 2) { + continue; + } + if (sv.getContaminationCandidate() > 0) { + sv.setEventScore(1); + sv.setEventType(5); + continue; + } + if (sv.getSelectedSa1().getSupport() > 19 && + sv.getSelectedSa1().getSecondarySupport() < 3) { + sv.setEventScore(1); + sv.setEventType(5); + continue; + } + if (sv.getSelectedSa2().getSupport() > 19 && + sv.getSelectedSa2().getSecondarySupport() < 3) { + sv.setEventScore(1); + sv.setEventType(5); + continue; + } + if (sv.getOverhang1lengthRatio() > 0.7 || + sv.getOverhang2lengthRatio() > 0.7) { + sv.setEventScore(1); + sv.setEventType(5); + continue; + } } } + return true; } - return true; - } - return false; -} -void -AnnotationProcessor::printFilteredResults(bool contaminationInControl, - int controlPrefilteringLevel) const { - if (controlPrefilteringLevel > 0) { - cout << "#controlMassiveInvPrefilteringLevel\t" - << controlPrefilteringLevel << endl; - } - if (massiveInvFilteringLevel > 0) { - cout << "#tumorMassiveInvFilteringLevel\t" << massiveInvFilteringLevel - << endl; - } - if (contaminationInControl) { - cout << "#likelyPathogenInControl\tTRUE" << endl; - } - if (contaminationObserved) { - cout << "#likelyPathogenInTumor\tTRUE" << endl; + return false; } - for (const auto &sv : filteredResults) { - if (!sv.isToRemove()) { - cout << sv.printMatch(overhangs); + void + AnnotationProcessor::printFilteredResults(bool contaminationInControl, + int controlPrefilteringLevel) const { + if (controlPrefilteringLevel > 0) { + cout << "#controlMassiveInvPrefilteringLevel\t" + << controlPrefilteringLevel << endl; + } + if (massiveInvFilteringLevel > 0) { + cout << "#tumorMassiveInvFilteringLevel\t" << massiveInvFilteringLevel + << endl; + } + if (contaminationInControl) { + cout << "#likelyPathogenInControl\tTRUE" << endl; + } + if (contaminationObserved) { + cout << "#likelyPathogenInTumor\tTRUE" << endl; + } + for (const auto &sv : filteredResults) { + if (!sv.isToRemove()) { + cout << sv.printMatch(overhangs); + } } } -} -void -AnnotationProcessor::printUnresolvedRareOverhangs( - vector> &mref) { - if (massiveInvFilteringLevel != 0) { - return; - } - sort(visitedLineIndices.begin(), visitedLineIndices.end()); - unordered_set visitedLineIndicesSet{visitedLineIndices.begin(), - visitedLineIndices.end()}; - for (const auto &tumorChromosome : tumorResults) { - for (const auto &bp : tumorChromosome) { - if (visitedLineIndicesSet.count(bp.getLineIndex())) { - continue; - } - if (bp.testOverhangBasedCandidacy()) { - auto mrefHits = searchMrefHitsNew( - bp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD, mref); - if (mrefHits.getNumHits() < SvEvent::GERMLINEDBLIMIT) { - auto germlineClonality = 1.0; - if (!NOCONTROLMODE) { - germlineClonality = - searchGermlineHitsNew( - bp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, - SvEvent::GERMLINEOFFSETTHRESHOLD) - .getClonality(); - } - string overhang{""}; - { - pair dummy{bp.getLineIndex(), ""}; - auto lower = lower_bound(overhangs.cbegin(), - overhangs.cend(), dummy); - if (lower != overhangs.cend()) { - if (lower->first == bp.getLineIndex()) { - overhang = lower->second; - } else if (next(lower) != overhangs.cend() && - next(lower)->first == - bp.getLineIndex()) { - overhang = next(lower)->second; - } else if (lower != overhangs.cbegin() && - prev(lower)->first == - bp.getLineIndex()) { - overhang = prev(lower)->second; + void + AnnotationProcessor::printUnresolvedRareOverhangs( + vector> &mref) { + if (massiveInvFilteringLevel != 0) { + return; + } + sort(visitedLineIndices.begin(), visitedLineIndices.end()); + unordered_set visitedLineIndicesSet{visitedLineIndices.begin(), + visitedLineIndices.end()}; + for (const auto &tumorChromosome : tumorResults) { + for (const auto &bp : tumorChromosome) { + if (visitedLineIndicesSet.count(bp.getLineIndex())) { + continue; + } + if (bp.testOverhangBasedCandidacy()) { + auto mrefHits = searchMrefHitsNew( + bp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD, mref); + if (mrefHits.getNumHits() < SvEvent::GERMLINEDBLIMIT) { + auto germlineClonality = 1.0; + if (!NOCONTROLMODE) { + germlineClonality = + searchGermlineHitsNew( + bp, SuppAlignmentAnno::DEFAULTREADLENGTH * 6, + SvEvent::GERMLINEOFFSETTHRESHOLD) + .getClonality(); + } + string overhang{""}; + { + pair dummy{bp.getLineIndex(), ""}; + auto lower = lower_bound(overhangs.cbegin(), + overhangs.cend(), dummy); + if (lower != overhangs.cend()) { + if (lower->first == bp.getLineIndex()) { + overhang = lower->second; + } else if (next(lower) != overhangs.cend() && + next(lower)->first == + bp.getLineIndex()) { + overhang = next(lower)->second; + } else if (lower != overhangs.cbegin() && + prev(lower)->first == + bp.getLineIndex()) { + overhang = prev(lower)->second; + } } } - } - if (!overhang.empty()) { - cout << bp.printOverhang( - germlineClonality, mrefHits.getNumHits(), overhang); + if (!overhang.empty()) { + cout << bp.printOverhang( + germlineClonality, mrefHits.getNumHits(), overhang); + } } } } } } -} } /* namespace sophia */ diff --git a/src/Breakpoint.cpp b/src/Breakpoint.cpp index ab0e985..88d35e1 100644 --- a/src/Breakpoint.cpp +++ b/src/Breakpoint.cpp @@ -34,501 +34,531 @@ namespace sophia { -using namespace std; + const string Breakpoint::COLUMNSSTR = boost::join( + vector{ + // + "#chr", "start", "end", // + "covTypes(pairedBreaksSoft,pairedBreaksHard,mateReadSupport," + "unpairedBreaksSoft,unpairedBreaksHard,shortIndelReads,normalSpans," + "lowQualSpansSoft,lowQualSpansHard,lowQualBreaksSoft,lowQualBreaksHard," + "repetitiveOverhangs)", // + "leftCoverage,rightCoverage", // + "suppAlignmentsDoubleSupport(primary,secondary,mate)", + "suppAlignments(primary,0,mate)", "significantOverhangs\n"}, + "\t"); + int Breakpoint::BPSUPPORTTHRESHOLD{}; + int Breakpoint::DEFAULTREADLENGTH{}; + int Breakpoint::DISCORDANTLOWQUALLEFTRANGE{}; + int Breakpoint::DISCORDANTLOWQUALRIGHTRANGE{}; + double Breakpoint::IMPROPERPAIRRATIO{0.0}; + bool Breakpoint::PROPERPAIRCOMPENSATIONMODE{false}; + int Breakpoint::bpindex{0}; -const string Breakpoint::COLUMNSSTR = boost::join( - vector{ - // - "#chr", "start", "end", // - "covTypes(pairedBreaksSoft,pairedBreaksHard,mateReadSupport," - "unpairedBreaksSoft,unpairedBreaksHard,shortIndelReads,normalSpans," - "lowQualSpansSoft,lowQualSpansHard,lowQualBreaksSoft,lowQualBreaksHard," - "repetitiveOverhangs)", // - "leftCoverage,rightCoverage", // - "suppAlignmentsDoubleSupport(primary,secondary,mate)", - "suppAlignments(primary,0,mate)", "significantOverhangs\n"}, - "\t"); -int Breakpoint::BPSUPPORTTHRESHOLD{}; -int Breakpoint::DEFAULTREADLENGTH{}; -int Breakpoint::DISCORDANTLOWQUALLEFTRANGE{}; -int Breakpoint::DISCORDANTLOWQUALRIGHTRANGE{}; -double Breakpoint::IMPROPERPAIRRATIO{0.0}; -bool Breakpoint::PROPERPAIRCOMPENSATIONMODE{false}; -int Breakpoint::bpindex{0}; + Breakpoint::Breakpoint(int chrIndexIn, int posIn) + : covFinalized{false}, + missingInfoBp{false}, + chrIndex{chrIndexIn}, + pos{posIn}, + normalSpans{0}, + lowQualSpansSoft{0}, + lowQualSpansHard{0}, + unpairedBreaksSoft{0}, + unpairedBreaksHard{0}, + breaksShortIndel{0}, + lowQualBreaksSoft{0}, + lowQualBreaksHard{0}, + repetitiveOverhangBreaks{0}, + pairedBreaksSoft{0}, + pairedBreaksHard{0}, + leftSideDiscordantCandidates{0}, + rightSideDiscordantCandidates{0}, + mateSupport{0}, + leftCoverage{0}, + rightCoverage{0}, + totalLowMapqHardClips{0}, + hitsInMref{-1}, + germline{false}, + poolLeft{}, + poolRight{}, + poolLowQualLeft{}, + poolLowQualRight{} {} -Breakpoint::Breakpoint(int chrIndexIn, int posIn) - : covFinalized{false}, - missingInfoBp{false}, - chrIndex{chrIndexIn}, - pos{posIn}, - normalSpans{0}, - lowQualSpansSoft{0}, - lowQualSpansHard{0}, - unpairedBreaksSoft{0}, - unpairedBreaksHard{0}, - breaksShortIndel{0}, - lowQualBreaksSoft{0}, - lowQualBreaksHard{0}, - repetitiveOverhangBreaks{0}, - pairedBreaksSoft{0}, - pairedBreaksHard{0}, - leftSideDiscordantCandidates{0}, - rightSideDiscordantCandidates{0}, - mateSupport{0}, - leftCoverage{0}, - rightCoverage{0}, - totalLowMapqHardClips{0}, - hitsInMref{-1}, - germline{false}, - poolLeft{}, - poolRight{}, - poolLowQualLeft{}, - poolLowQualRight{} {} - -template -inline void -Breakpoint::cleanUpVector(vector &objectPool) { - // cerr << "cleaning up" << endl; - while (!objectPool.empty() && objectPool.back().isToRemove()) { - objectPool.pop_back(); - } - for (auto saIt = objectPool.begin(); saIt != objectPool.end(); ++saIt) { - if (saIt->isToRemove()) { - swap(*saIt, objectPool.back()); - } + template + inline void + Breakpoint::cleanUpVector(vector &objectPool) { + // cerr << "cleaning up" << endl; while (!objectPool.empty() && objectPool.back().isToRemove()) { objectPool.pop_back(); } + for (auto saIt = objectPool.begin(); saIt != objectPool.end(); ++saIt) { + if (saIt->isToRemove()) { + swap(*saIt, objectPool.back()); + } + while (!objectPool.empty() && objectPool.back().isToRemove()) { + objectPool.pop_back(); + } + } + // cerr << "done" << endl; } - // cerr << "done" << endl; -} -void -Breakpoint::addSoftAlignment(shared_ptr alignmentIn) { - if (!alignmentIn->isSupplementary()) { - if (supportingSoftAlignments.size() <= MAXPERMISSIBLESOFTCLIPS) { - supportingSoftAlignments.push_back(alignmentIn); + void + Breakpoint::addSoftAlignment(shared_ptr alignmentIn) { + if (!alignmentIn->isSupplementary()) { + if (supportingSoftAlignments.size() <= MAXPERMISSIBLESOFTCLIPS) { + supportingSoftAlignments.push_back(alignmentIn); + } } } -} -void -Breakpoint::addHardAlignment(shared_ptr alignmentIn) { - if (alignmentIn->isSupplementary()) { - if (!(alignmentIn->isLowMapq() || alignmentIn->isNullMapq())) { - if (supportingHardAlignments.size() <= MAXPERMISSIBLEHARDCLIPS) { - supportingHardAlignments.push_back(alignmentIn); - } - } else { - if (totalLowMapqHardClips < MAXPERMISSIBLELOWMAPQHARDCLIPS) { - supportingHardLowMapqAlignments.push_back(alignmentIn); - ++totalLowMapqHardClips; + void + Breakpoint::addHardAlignment(shared_ptr alignmentIn) { + if (alignmentIn->isSupplementary()) { + if (!(alignmentIn->isLowMapq() || alignmentIn->isNullMapq())) { + if (supportingHardAlignments.size() <= MAXPERMISSIBLEHARDCLIPS) { + supportingHardAlignments.push_back(alignmentIn); + } } else { - supportingHardLowMapqAlignments.clear(); + if (totalLowMapqHardClips < MAXPERMISSIBLELOWMAPQHARDCLIPS) { + supportingHardLowMapqAlignments.push_back(alignmentIn); + ++totalLowMapqHardClips; + } else { + supportingHardLowMapqAlignments.clear(); + } } } } -} -bool -Breakpoint::finalizeBreakpoint( - const deque &discordantAlignmentsPool, - const deque &discordantLowQualAlignmentsPool, - const deque &discordantAlignmentCandidatesPool) { - auto overhangStr = string(); - auto eventTotal = - unpairedBreaksSoft + unpairedBreaksHard + breaksShortIndel; - auto artifactTotal = - lowQualBreaksSoft + lowQualSpansSoft + lowQualSpansHard; - if ((eventTotal + artifactTotal > 50) && - (artifactTotal / (0.0 + eventTotal + artifactTotal)) > 0.85) { - ++bpindex; - missingInfoBp = true; - } else if (static_cast(supportingSoftAlignments.size()) == - MAXPERMISSIBLESOFTCLIPS && - eventTotal + normalSpans + artifactTotal > - MAXPERMISSIBLEHARDCLIPS * 20) { - ++bpindex; - missingInfoBp = true; - } else { - fillMatePool(discordantAlignmentsPool, discordantLowQualAlignmentsPool, - discordantAlignmentCandidatesPool); - if (eventTotal < BPSUPPORTTHRESHOLD && - static_cast(poolLeft.size()) < BPSUPPORTTHRESHOLD && - static_cast(poolLowQualLeft.size()) < BPSUPPORTTHRESHOLD && - static_cast(poolRight.size()) < BPSUPPORTTHRESHOLD && - static_cast(poolLowQualRight.size()) < BPSUPPORTTHRESHOLD) { - if (artifactTotal < normalSpans) { - return false; + bool + Breakpoint::finalizeBreakpoint( + const deque &discordantAlignmentsPool, + const deque &discordantLowQualAlignmentsPool, + const deque &discordantAlignmentCandidatesPool) { + auto overhangStr = string(); + auto eventTotal = + unpairedBreaksSoft + unpairedBreaksHard + breaksShortIndel; + auto artifactTotal = + lowQualBreaksSoft + lowQualSpansSoft + lowQualSpansHard; + if ((eventTotal + artifactTotal > 50) && + (artifactTotal / (0.0 + eventTotal + artifactTotal)) > 0.85) { + ++bpindex; + missingInfoBp = true; + } else if (static_cast(supportingSoftAlignments.size()) == + MAXPERMISSIBLESOFTCLIPS && + eventTotal + normalSpans + artifactTotal > + MAXPERMISSIBLEHARDCLIPS * 20) { + ++bpindex; + missingInfoBp = true; + } else { + fillMatePool(discordantAlignmentsPool, discordantLowQualAlignmentsPool, + discordantAlignmentCandidatesPool); + if (eventTotal < BPSUPPORTTHRESHOLD && + static_cast(poolLeft.size()) < BPSUPPORTTHRESHOLD && + static_cast(poolLowQualLeft.size()) < BPSUPPORTTHRESHOLD && + static_cast(poolRight.size()) < BPSUPPORTTHRESHOLD && + static_cast(poolLowQualRight.size()) < BPSUPPORTTHRESHOLD) { + if (artifactTotal < normalSpans) { + return false; + } else { + ++bpindex; + missingInfoBp = true; + } } else { - ++bpindex; - missingInfoBp = true; + overhangStr = finalizeOverhangs(); + detectDoubleSupportSupps(); + collectMateSupport(); } - } else { - overhangStr = finalizeOverhangs(); - detectDoubleSupportSupps(); - collectMateSupport(); } - } - if (eventTotal + mateSupport + artifactTotal < BPSUPPORTTHRESHOLD || - (eventTotal + artifactTotal < BPSUPPORTTHRESHOLD && - doubleSidedMatches.empty() && supplementsPrimary.empty())) { - return false; - } - if (missingInfoBp || - (doubleSidedMatches.empty() && supplementsPrimary.empty() && - overhangStr.size() < 2)) { - auto artifactTotal2 = lowQualBreaksSoft + lowQualSpansHard + - lowQualSpansSoft + repetitiveOverhangBreaks; - auto artifactTotal2Relaxed = - lowQualBreaksSoft + lowQualSpansSoft + repetitiveOverhangBreaks; - auto eventTotal2 = pairedBreaksSoft + pairedBreaksHard + - unpairedBreaksSoft + unpairedBreaksHard + - breaksShortIndel; - auto eventTotal2Strict = - pairedBreaksSoft + unpairedBreaksSoft + pairedBreaksHard; - auto covCriterion = (eventTotal2 + artifactTotal2) > 10; - if (!(covCriterion && eventTotal2Strict + artifactTotal2Relaxed > 0)) { + if (eventTotal + mateSupport + artifactTotal < BPSUPPORTTHRESHOLD || + (eventTotal + artifactTotal < BPSUPPORTTHRESHOLD && + doubleSidedMatches.empty() && supplementsPrimary.empty())) { return false; } + if (missingInfoBp || + (doubleSidedMatches.empty() && supplementsPrimary.empty() && + overhangStr.size() < 2)) { + auto artifactTotal2 = lowQualBreaksSoft + lowQualSpansHard + + lowQualSpansSoft + repetitiveOverhangBreaks; + auto artifactTotal2Relaxed = + lowQualBreaksSoft + lowQualSpansSoft + repetitiveOverhangBreaks; + auto eventTotal2 = pairedBreaksSoft + pairedBreaksHard + + unpairedBreaksSoft + unpairedBreaksHard + + breaksShortIndel; + auto eventTotal2Strict = + pairedBreaksSoft + unpairedBreaksSoft + pairedBreaksHard; + auto covCriterion = (eventTotal2 + artifactTotal2) > 10; + if (!(covCriterion && eventTotal2Strict + artifactTotal2Relaxed > 0)) { + return false; + } + } + printBreakpointReport(overhangStr); + return true; } - printBreakpointReport(overhangStr); - return true; -} -void -Breakpoint::printBreakpointReport(const string &overhangStr) { - string res{}; - res.reserve(350); - res.append(GlobalAppConfig::getInstance().getChrConverter().indexToChr[chrIndex]).append("\t"); - res.append(strtk::type_to_string(pos)).append("\t"); - res.append(strtk::type_to_string(pos + 1)).append("\t"); + void + Breakpoint::printBreakpointReport(const string &overhangStr) { + string res{}; + res.reserve(350); + res.append(GlobalAppConfig::getInstance().getChrConverter().indexToChr[chrIndex]).append("\t"); + res.append(strtk::type_to_string(pos)).append("\t"); + res.append(strtk::type_to_string(pos + 1)).append("\t"); - res.append(strtk::type_to_string(pairedBreaksSoft)).append(","); - res.append(strtk::type_to_string(pairedBreaksHard)).append(","); - res.append(strtk::type_to_string(mateSupport)).append(","); - res.append(strtk::type_to_string(unpairedBreaksSoft)).append(","); - res.append(strtk::type_to_string(unpairedBreaksHard)).append(","); - res.append(strtk::type_to_string(breaksShortIndel)).append(","); + res.append(strtk::type_to_string(pairedBreaksSoft)).append(","); + res.append(strtk::type_to_string(pairedBreaksHard)).append(","); + res.append(strtk::type_to_string(mateSupport)).append(","); + res.append(strtk::type_to_string(unpairedBreaksSoft)).append(","); + res.append(strtk::type_to_string(unpairedBreaksHard)).append(","); + res.append(strtk::type_to_string(breaksShortIndel)).append(","); - res.append(strtk::type_to_string(normalSpans)).append(","); + res.append(strtk::type_to_string(normalSpans)).append(","); - res.append(strtk::type_to_string(lowQualSpansSoft)).append(","); - res.append(strtk::type_to_string(lowQualSpansHard)).append(","); - res.append(strtk::type_to_string(lowQualBreaksSoft)).append(","); - res.append(strtk::type_to_string(lowQualBreaksHard)).append(","); - res.append(strtk::type_to_string(repetitiveOverhangBreaks)) - .append("\t"); + res.append(strtk::type_to_string(lowQualSpansSoft)).append(","); + res.append(strtk::type_to_string(lowQualSpansHard)).append(","); + res.append(strtk::type_to_string(lowQualBreaksSoft)).append(","); + res.append(strtk::type_to_string(lowQualBreaksHard)).append(","); + res.append(strtk::type_to_string(repetitiveOverhangBreaks)) + .append("\t"); - res.append(strtk::type_to_string(leftCoverage)).append(","); - res.append(strtk::type_to_string(rightCoverage)).append("\t"); - if (missingInfoBp) { - res.append("#\t#\t#\n"); - } else { - collapseSuppRange(res, doubleSidedMatches); - res.append("\t"); - collapseSuppRange(res, supplementsPrimary); - res.append("\t"); - if (overhangStr.empty()) { - res.append(".").append("\n"); + res.append(strtk::type_to_string(leftCoverage)).append(","); + res.append(strtk::type_to_string(rightCoverage)).append("\t"); + if (missingInfoBp) { + res.append("#\t#\t#\n"); } else { - res.append(overhangStr).append("\n"); + collapseSuppRange(res, doubleSidedMatches); + res.append("\t"); + collapseSuppRange(res, supplementsPrimary); + res.append("\t"); + if (overhangStr.empty()) { + res.append(".").append("\n"); + } else { + res.append(overhangStr).append("\n"); + } } + cout << res; } - cout << res; -} -void -Breakpoint::collapseSuppRange(string &res, - const vector &vec) const { - if (vec.empty()) { - res.append("."); - } else { - for (const auto &suppAlignment : vec) { - res.append(suppAlignment.print()).append(";"); + void + Breakpoint::collapseSuppRange(string &res, + const vector &vec) const { + if (vec.empty()) { + res.append("."); + } else { + for (const auto &suppAlignment : vec) { + res.append(suppAlignment.print()).append(";"); + } + res.pop_back(); } - res.pop_back(); } -} -string -Breakpoint::finalizeOverhangs() { - ++bpindex; - for (auto i = 0u; i < supportingSoftAlignments.size(); ++i) { - supportingSoftAlignments[i]->setChosenBp(pos, i); - if (supportingSoftAlignments[i]->assessOutlierMateDistance()) { - if (supportingSoftAlignments[i]->getMateChrIndex() < 1002) { - if (supportingSoftAlignments[i]->isOverhangEncounteredM()) { - if (!(supportingSoftAlignments[i]->isNullMapq() || - supportingSoftAlignments[i]->isLowMapq())) { - poolLeft.emplace_back( - supportingSoftAlignments[i]->getStartPos(), - supportingSoftAlignments[i]->getEndPos(), - supportingSoftAlignments[i]->getMateChrIndex(), - supportingSoftAlignments[i]->getMatePos(), 0, - supportingSoftAlignments[i]->isInvertedMate()); - } else { - poolLowQualLeft.emplace_back( - supportingSoftAlignments[i]->getStartPos(), - supportingSoftAlignments[i]->getEndPos(), - supportingSoftAlignments[i]->getMateChrIndex(), - supportingSoftAlignments[i]->getMatePos(), 0, - supportingSoftAlignments[i]->isInvertedMate()); - } - } else { - if (!(supportingSoftAlignments[i]->isNullMapq() || - supportingSoftAlignments[i]->isLowMapq())) { - poolRight.emplace_back( - supportingSoftAlignments[i]->getStartPos(), - supportingSoftAlignments[i]->getEndPos(), - supportingSoftAlignments[i]->getMateChrIndex(), - supportingSoftAlignments[i]->getMatePos(), 0, - supportingSoftAlignments[i]->isInvertedMate()); + string + Breakpoint::finalizeOverhangs() { + ++bpindex; + for (auto i = 0u; i < supportingSoftAlignments.size(); ++i) { + supportingSoftAlignments[i]->setChosenBp(pos, i); + if (supportingSoftAlignments[i]->assessOutlierMateDistance()) { + if (supportingSoftAlignments[i]->getMateChrIndex() < 1002) { + if (supportingSoftAlignments[i]->isOverhangEncounteredM()) { + if (!(supportingSoftAlignments[i]->isNullMapq() || + supportingSoftAlignments[i]->isLowMapq())) { + poolLeft.emplace_back( + supportingSoftAlignments[i]->getStartPos(), + supportingSoftAlignments[i]->getEndPos(), + supportingSoftAlignments[i]->getMateChrIndex(), + supportingSoftAlignments[i]->getMatePos(), 0, + supportingSoftAlignments[i]->isInvertedMate()); + } else { + poolLowQualLeft.emplace_back( + supportingSoftAlignments[i]->getStartPos(), + supportingSoftAlignments[i]->getEndPos(), + supportingSoftAlignments[i]->getMateChrIndex(), + supportingSoftAlignments[i]->getMatePos(), 0, + supportingSoftAlignments[i]->isInvertedMate()); + } } else { - poolLowQualRight.emplace_back( - supportingSoftAlignments[i]->getStartPos(), - supportingSoftAlignments[i]->getEndPos(), - supportingSoftAlignments[i]->getMateChrIndex(), - supportingSoftAlignments[i]->getMatePos(), 0, - supportingSoftAlignments[i]->isInvertedMate()); + if (!(supportingSoftAlignments[i]->isNullMapq() || + supportingSoftAlignments[i]->isLowMapq())) { + poolRight.emplace_back( + supportingSoftAlignments[i]->getStartPos(), + supportingSoftAlignments[i]->getEndPos(), + supportingSoftAlignments[i]->getMateChrIndex(), + supportingSoftAlignments[i]->getMatePos(), 0, + supportingSoftAlignments[i]->isInvertedMate()); + } else { + poolLowQualRight.emplace_back( + supportingSoftAlignments[i]->getStartPos(), + supportingSoftAlignments[i]->getEndPos(), + supportingSoftAlignments[i]->getMateChrIndex(), + supportingSoftAlignments[i]->getMatePos(), 0, + supportingSoftAlignments[i]->isInvertedMate()); + } } } } } - } - vector supplementsPrimaryTmp{}; - sort(supportingSoftAlignments.begin(), supportingSoftAlignments.end(), - [](const shared_ptr &a, const shared_ptr &b) { - return a->getOverhangLength() < b->getOverhangLength(); - }); - vector> supportingSoftParentAlignments{}; - while (!supportingSoftAlignments.empty()) { - auto substrCheck = false; - auto tmpSas = supportingSoftAlignments.back()->generateSuppAlignments( - chrIndex, pos); - for (auto overhangParent : supportingSoftParentAlignments) { - if (matchDetector(overhangParent, - supportingSoftAlignments.back())) { - substrCheck = true; - overhangParent->addChildNode( - supportingSoftAlignments.back()->getOriginIndex()); - overhangParent->addSupplementaryAlignments(tmpSas); + vector supplementsPrimaryTmp{}; + sort(supportingSoftAlignments.begin(), supportingSoftAlignments.end(), + [](const shared_ptr &a, const shared_ptr &b) { + return a->getOverhangLength() < b->getOverhangLength(); + }); + vector> supportingSoftParentAlignments{}; + while (!supportingSoftAlignments.empty()) { + auto substrCheck = false; + auto tmpSas = supportingSoftAlignments.back()->generateSuppAlignments( + chrIndex, pos); + for (auto overhangParent : supportingSoftParentAlignments) { + if (matchDetector(overhangParent, + supportingSoftAlignments.back())) { + substrCheck = true; + overhangParent->addChildNode( + supportingSoftAlignments.back()->getOriginIndex()); + overhangParent->addSupplementaryAlignments(tmpSas); + } } - } - if (!substrCheck) { - auto allDistant = - !tmpSas.empty() && - all_of(cbegin(tmpSas), cend(tmpSas), - [](const SuppAlignment &sa) { return sa.isDistant(); }); - if (allDistant || supportingSoftAlignments.back() - ->overhangComplexityMaskRatio() <= 0.5) { - if (supportingSoftAlignments.back()->getOverhangLength() >= - 20) { - supportingSoftAlignments.back()->addSupplementaryAlignments( - tmpSas); - supportingSoftParentAlignments.push_back( - supportingSoftAlignments.back()); - } else { - for (const auto &sa : tmpSas) { - auto it = - find_if(supplementsPrimaryTmp.begin(), - supplementsPrimaryTmp.end(), - [&](const SuppAlignment &suppAlignment) { - return suppAlignment.saCloseness(sa, 5); - }); - if (it == supplementsPrimaryTmp.end()) { - supplementsPrimaryTmp.push_back(sa); - } else { - if (it->isFuzzy() && !sa.isFuzzy()) { - it->removeFuzziness(sa); - } else if (it->isFuzzy() && sa.isFuzzy()) { - it->extendSuppAlignment(sa.getPos(), - sa.getExtendedPos()); - } - it->addSupportingIndices( - supportingSoftAlignments.back() - ->getChildrenNodes()); - if (it->isNullMapqSource() && - !supportingSoftAlignments.back() - ->isNullMapq()) { - it->setNullMapqSource(false); + if (!substrCheck) { + auto allDistant = + !tmpSas.empty() && + all_of(cbegin(tmpSas), cend(tmpSas), + [](const SuppAlignment &sa) { return sa.isDistant(); }); + if (allDistant || supportingSoftAlignments.back() + ->overhangComplexityMaskRatio() <= 0.5) { + if (supportingSoftAlignments.back()->getOverhangLength() >= + 20) { + supportingSoftAlignments.back()->addSupplementaryAlignments( + tmpSas); + supportingSoftParentAlignments.push_back( + supportingSoftAlignments.back()); + } else { + for (const auto &sa : tmpSas) { + auto it = + find_if(supplementsPrimaryTmp.begin(), + supplementsPrimaryTmp.end(), + [&](const SuppAlignment &suppAlignment) { + return suppAlignment.saCloseness(sa, 5); + }); + if (it == supplementsPrimaryTmp.end()) { + supplementsPrimaryTmp.push_back(sa); + } else { + if (it->isFuzzy() && !sa.isFuzzy()) { + it->removeFuzziness(sa); + } else if (it->isFuzzy() && sa.isFuzzy()) { + it->extendSuppAlignment(sa.getPos(), + sa.getExtendedPos()); + } + it->addSupportingIndices( + supportingSoftAlignments.back() + ->getChildrenNodes()); + if (it->isNullMapqSource() && + !supportingSoftAlignments.back() + ->isNullMapq()) { + it->setNullMapqSource(false); + } } } } + } else { + --unpairedBreaksSoft; + ++repetitiveOverhangBreaks; } - } else { - --unpairedBreaksSoft; - ++repetitiveOverhangBreaks; } - } - supportingSoftAlignments.pop_back(); - } - string consensusOverhangsTmp{}; - consensusOverhangsTmp.reserve(250); - { - auto i = 1; - auto indexStr = strtk::type_to_string(bpindex); - for (const auto &overhangParent : supportingSoftParentAlignments) { - if (static_cast(overhangParent->getChildrenNodes().size()) >= - BPSUPPORTTHRESHOLD) { - consensusOverhangsTmp.append(">") - .append(indexStr) - .append("_") - .append(strtk::type_to_string(i)) - .append(":") - .append(overhangParent->printOverhang()) - .append(";"); - ++i; - } - for (const auto &sa : - overhangParent->getSupplementaryAlignments()) { - if (sa.isToRemove()) { - continue; + supportingSoftAlignments.pop_back(); + } + string consensusOverhangsTmp{}; + consensusOverhangsTmp.reserve(250); + { + auto i = 1; + auto indexStr = strtk::type_to_string(bpindex); + for (const auto &overhangParent : supportingSoftParentAlignments) { + if (static_cast(overhangParent->getChildrenNodes().size()) >= + BPSUPPORTTHRESHOLD) { + consensusOverhangsTmp.append(">") + .append(indexStr) + .append("_") + .append(strtk::type_to_string(i)) + .append(":") + .append(overhangParent->printOverhang()) + .append(";"); + ++i; } - auto it = find_if(supplementsPrimary.begin(), - supplementsPrimary.end(), - [&](const SuppAlignment &suppAlignment) { - return suppAlignment.saCloseness(sa, 5); - }); - if (it == supplementsPrimary.end()) { - supplementsPrimary.push_back(sa); - supplementsPrimary.back().addSupportingIndices( - overhangParent->getChildrenNodes()); - } else { - if (it->isFuzzy() && !sa.isFuzzy()) { - it->removeFuzziness(sa); - } else if (it->isFuzzy() && sa.isFuzzy()) { - it->extendSuppAlignment(sa.getPos(), - sa.getExtendedPos()); + for (const auto &sa : + overhangParent->getSupplementaryAlignments()) { + if (sa.isToRemove()) { + continue; } - it->addSupportingIndices( - overhangParent->getChildrenNodes()); - if (it->isNullMapqSource() && - !supplementsPrimary.back().isNullMapqSource()) { - it->setNullMapqSource(false); + auto it = find_if(supplementsPrimary.begin(), + supplementsPrimary.end(), + [&](const SuppAlignment &suppAlignment) { + return suppAlignment.saCloseness(sa, 5); + }); + if (it == supplementsPrimary.end()) { + supplementsPrimary.push_back(sa); + supplementsPrimary.back().addSupportingIndices( + overhangParent->getChildrenNodes()); + } else { + if (it->isFuzzy() && !sa.isFuzzy()) { + it->removeFuzziness(sa); + } else if (it->isFuzzy() && sa.isFuzzy()) { + it->extendSuppAlignment(sa.getPos(), + sa.getExtendedPos()); + } + it->addSupportingIndices( + overhangParent->getChildrenNodes()); + if (it->isNullMapqSource() && + !supplementsPrimary.back().isNullMapqSource()) { + it->setNullMapqSource(false); + } } } } - } - supportingSoftParentAlignments.clear(); - if (!consensusOverhangsTmp.empty()) { - consensusOverhangsTmp.pop_back(); - } else { - return string(); - } - } - for (const auto &sa : supplementsPrimaryTmp) { - auto it = find_if(supplementsPrimary.begin(), supplementsPrimary.end(), - [&](const SuppAlignment &suppAlignment) { - return suppAlignment.saCloseness(sa, 5); - }); - if (it == supplementsPrimary.end()) { - supplementsPrimary.push_back(sa); - } else { - if (it->isFuzzy() && !sa.isFuzzy()) { - it->removeFuzziness(sa); - } else if (it->isFuzzy() && sa.isFuzzy()) { - it->extendSuppAlignment(sa.getPos(), sa.getExtendedPos()); + supportingSoftParentAlignments.clear(); + if (!consensusOverhangsTmp.empty()) { + consensusOverhangsTmp.pop_back(); + } else { + return string(); } - it->addSupportingIndices(sa.getSupportingIndices()); - if (it->isNullMapqSource() && !sa.isNullMapqSource()) { - it->setNullMapqSource(false); + } + for (const auto &sa : supplementsPrimaryTmp) { + auto it = find_if(supplementsPrimary.begin(), supplementsPrimary.end(), + [&](const SuppAlignment &suppAlignment) { + return suppAlignment.saCloseness(sa, 5); + }); + if (it == supplementsPrimary.end()) { + supplementsPrimary.push_back(sa); + } else { + if (it->isFuzzy() && !sa.isFuzzy()) { + it->removeFuzziness(sa); + } else if (it->isFuzzy() && sa.isFuzzy()) { + it->extendSuppAlignment(sa.getPos(), sa.getExtendedPos()); + } + it->addSupportingIndices(sa.getSupportingIndices()); + if (it->isNullMapqSource() && !sa.isNullMapqSource()) { + it->setNullMapqSource(false); + } } } + return consensusOverhangsTmp; } - return consensusOverhangsTmp; -} -bool -Breakpoint::matchDetector(const shared_ptr &longAlignment, - const shared_ptr &shortAlignment) const { - if (longAlignment->isOverhangEncounteredM() != - shortAlignment->isOverhangEncounteredM()) { - return false; - } - auto mismatches = 0; - char cLong{}; - char cShort{}; - auto shortS = shortAlignment->getOverhangLength(); - auto longStart = longAlignment->getOverhangStartIndex(); - auto shortStart = shortAlignment->getOverhangStartIndex(); - const auto pointerToLongSeq = &longAlignment->getSamLine(); - const auto pointerToShortSeq = &shortAlignment->getSamLine(); - if (!longAlignment->isOverhangEncounteredM() && - !shortAlignment->isOverhangEncounteredM()) { - auto lenDiff = longAlignment->getOverhangLength() - shortS; - for (int i = shortS - 1; i >= 0; --i) { - cLong = (*pointerToLongSeq)[longStart + i + lenDiff]; - if (cLong == 'N') - continue; - cShort = (*pointerToShortSeq)[shortStart + i]; - if (cShort == 'N') - continue; - if (cLong != cShort) { - ++mismatches; - if (mismatches > PERMISSIBLEMISMATCHES) - return false; - } + bool + Breakpoint::matchDetector(const shared_ptr &longAlignment, + const shared_ptr &shortAlignment) const { + if (longAlignment->isOverhangEncounteredM() != + shortAlignment->isOverhangEncounteredM()) { + return false; } - return true; - } else if (longAlignment->isOverhangEncounteredM() && - shortAlignment->isOverhangEncounteredM()) { - for (auto i = 0; i < shortS; ++i) { - cLong = (*pointerToLongSeq)[longStart + i]; - if (cLong == 'N') - continue; - cShort = (*pointerToShortSeq)[shortStart + i]; - if (cShort == 'N') - continue; - if (cLong != cShort) { - ++mismatches; - if (mismatches > PERMISSIBLEMISMATCHES) - return false; + auto mismatches = 0; + char cLong{}; + char cShort{}; + auto shortS = shortAlignment->getOverhangLength(); + auto longStart = longAlignment->getOverhangStartIndex(); + auto shortStart = shortAlignment->getOverhangStartIndex(); + const auto pointerToLongSeq = &longAlignment->getSamLine(); + const auto pointerToShortSeq = &shortAlignment->getSamLine(); + if (!longAlignment->isOverhangEncounteredM() && + !shortAlignment->isOverhangEncounteredM()) { + auto lenDiff = longAlignment->getOverhangLength() - shortS; + for (int i = shortS - 1; i >= 0; --i) { + cLong = (*pointerToLongSeq)[longStart + i + lenDiff]; + if (cLong == 'N') + continue; + cShort = (*pointerToShortSeq)[shortStart + i]; + if (cShort == 'N') + continue; + if (cLong != cShort) { + ++mismatches; + if (mismatches > PERMISSIBLEMISMATCHES) + return false; + } } + return true; + } else if (longAlignment->isOverhangEncounteredM() && + shortAlignment->isOverhangEncounteredM()) { + for (auto i = 0; i < shortS; ++i) { + cLong = (*pointerToLongSeq)[longStart + i]; + if (cLong == 'N') + continue; + cShort = (*pointerToShortSeq)[shortStart + i]; + if (cShort == 'N') + continue; + if (cLong != cShort) { + ++mismatches; + if (mismatches > PERMISSIBLEMISMATCHES) + return false; + } + } + return true; } - return true; + return false; } - return false; -} -void -Breakpoint::detectDoubleSupportSupps() { - vector saHardTmpLowQual; - { - auto i = 0u; - for (; i < supportingHardAlignments.size(); ++i) { - supportingHardAlignments[i]->setChosenBp(pos, i); - } - for (auto hardAlignment : supportingHardAlignments) { - for (const auto &sa : - hardAlignment->generateSuppAlignments(chrIndex, pos)) { - if (!(sa.isInverted() && sa.getPos() == pos && - sa.getChrIndex() == chrIndex)) { - supplementsSecondary.push_back(sa); - if (supplementsSecondary.back().isDistant()) { - if (supplementsSecondary.back().isEncounteredM()) { - if (!(hardAlignment->isNullMapq() || - hardAlignment->isLowMapq())) { - poolLeft.emplace_back( - hardAlignment->getStartPos(), - hardAlignment->getEndPos(), - hardAlignment->getMateChrIndex(), - hardAlignment->getMatePos(), 1, - hardAlignment->isInvertedMate()); + void + Breakpoint::detectDoubleSupportSupps() { + vector saHardTmpLowQual; + { + auto i = 0u; + for (; i < supportingHardAlignments.size(); ++i) { + supportingHardAlignments[i]->setChosenBp(pos, i); + } + for (auto hardAlignment : supportingHardAlignments) { + for (const auto &sa : + hardAlignment->generateSuppAlignments(chrIndex, pos)) { + if (!(sa.isInverted() && sa.getPos() == pos && + sa.getChrIndex() == chrIndex)) { + supplementsSecondary.push_back(sa); + if (supplementsSecondary.back().isDistant()) { + if (supplementsSecondary.back().isEncounteredM()) { + if (!(hardAlignment->isNullMapq() || + hardAlignment->isLowMapq())) { + poolLeft.emplace_back( + hardAlignment->getStartPos(), + hardAlignment->getEndPos(), + hardAlignment->getMateChrIndex(), + hardAlignment->getMatePos(), 1, + hardAlignment->isInvertedMate()); + } else { + poolLowQualLeft.emplace_back( + hardAlignment->getStartPos(), + hardAlignment->getEndPos(), + hardAlignment->getMateChrIndex(), + hardAlignment->getMatePos(), 1, + hardAlignment->isInvertedMate()); + } + } else { - poolLowQualLeft.emplace_back( - hardAlignment->getStartPos(), - hardAlignment->getEndPos(), - hardAlignment->getMateChrIndex(), - hardAlignment->getMatePos(), 1, - hardAlignment->isInvertedMate()); + if (!(hardAlignment->isNullMapq() || + hardAlignment->isLowMapq())) { + poolRight.emplace_back( + hardAlignment->getStartPos(), + hardAlignment->getEndPos(), + hardAlignment->getMateChrIndex(), + hardAlignment->getMatePos(), 1, + hardAlignment->isInvertedMate()); + } else { + poolLowQualRight.emplace_back( + hardAlignment->getStartPos(), + hardAlignment->getEndPos(), + hardAlignment->getMateChrIndex(), + hardAlignment->getMatePos(), 1, + hardAlignment->isInvertedMate()); + } } + } + } + } + } + supportingHardAlignments.clear(); + for (auto j = 0u; j < supportingHardLowMapqAlignments.size(); ++j) { + supportingHardLowMapqAlignments[j]->setChosenBp(pos, i + j); + } - } else { - if (!(hardAlignment->isNullMapq() || - hardAlignment->isLowMapq())) { - poolRight.emplace_back( + for (auto hardAlignment : supportingHardLowMapqAlignments) { + for (const auto &sa : + hardAlignment->generateSuppAlignments(chrIndex, pos)) { + if (!(sa.isInverted() && sa.getPos() == pos && + sa.getChrIndex() == chrIndex)) { + saHardTmpLowQual.push_back(sa); + if (saHardTmpLowQual.back().isDistant()) { + if (saHardTmpLowQual.back().isEncounteredM()) { + poolLowQualLeft.emplace_back( hardAlignment->getStartPos(), hardAlignment->getEndPos(), hardAlignment->getMateChrIndex(), @@ -546,872 +576,838 @@ Breakpoint::detectDoubleSupportSupps() { } } } - } - supportingHardAlignments.clear(); - for (auto j = 0u; j < supportingHardLowMapqAlignments.size(); ++j) { - supportingHardLowMapqAlignments[j]->setChosenBp(pos, i + j); - } - - for (auto hardAlignment : supportingHardLowMapqAlignments) { - for (const auto &sa : - hardAlignment->generateSuppAlignments(chrIndex, pos)) { - if (!(sa.isInverted() && sa.getPos() == pos && - sa.getChrIndex() == chrIndex)) { - saHardTmpLowQual.push_back(sa); - if (saHardTmpLowQual.back().isDistant()) { - if (saHardTmpLowQual.back().isEncounteredM()) { - poolLowQualLeft.emplace_back( - hardAlignment->getStartPos(), - hardAlignment->getEndPos(), - hardAlignment->getMateChrIndex(), - hardAlignment->getMatePos(), 1, - hardAlignment->isInvertedMate()); - } else { - poolLowQualRight.emplace_back( - hardAlignment->getStartPos(), - hardAlignment->getEndPos(), - hardAlignment->getMateChrIndex(), - hardAlignment->getMatePos(), 1, - hardAlignment->isInvertedMate()); + supportingHardLowMapqAlignments.clear(); + } + { + vector lowMapqHardSupportWhitelistIndices{}; + for (auto primarySupptIt = supplementsPrimary.begin(); + primarySupptIt != supplementsPrimary.end(); ++primarySupptIt) { + auto foundMatch = false; + for (auto secondarySuppIt = supplementsSecondary.begin(); + secondarySuppIt != supplementsSecondary.end(); + ++secondarySuppIt) { + if (primarySupptIt->saCloseness(*secondarySuppIt, 100)) { + if (primarySupptIt->isFuzzy() && + !secondarySuppIt->isFuzzy()) { + primarySupptIt->removeFuzziness(*secondarySuppIt); + } else if (primarySupptIt->isFuzzy() && + secondarySuppIt->isFuzzy()) { + primarySupptIt->extendSuppAlignment( + secondarySuppIt->getPos(), + secondarySuppIt->getExtendedPos()); } + foundMatch = true; + secondarySuppIt->setToRemove(true); + primarySupptIt->addSecondarySupportIndices( + secondarySuppIt->getSupportingIndicesSecondary()); } } - } - } - supportingHardLowMapqAlignments.clear(); - } - { - vector lowMapqHardSupportWhitelistIndices{}; - for (auto primarySupptIt = supplementsPrimary.begin(); - primarySupptIt != supplementsPrimary.end(); ++primarySupptIt) { - auto foundMatch = false; - for (auto secondarySuppIt = supplementsSecondary.begin(); - secondarySuppIt != supplementsSecondary.end(); - ++secondarySuppIt) { - if (primarySupptIt->saCloseness(*secondarySuppIt, 100)) { - if (primarySupptIt->isFuzzy() && - !secondarySuppIt->isFuzzy()) { - primarySupptIt->removeFuzziness(*secondarySuppIt); - } else if (primarySupptIt->isFuzzy() && - secondarySuppIt->isFuzzy()) { - primarySupptIt->extendSuppAlignment( - secondarySuppIt->getPos(), - secondarySuppIt->getExtendedPos()); + for (auto secondarySuppIt = saHardTmpLowQual.begin(); + secondarySuppIt != saHardTmpLowQual.end(); ++secondarySuppIt) { + if (primarySupptIt->saCloseness(*secondarySuppIt, 100)) { + if (primarySupptIt->isFuzzy() && + !secondarySuppIt->isFuzzy()) { + primarySupptIt->removeFuzziness(*secondarySuppIt); + } else if (primarySupptIt->isFuzzy() && + secondarySuppIt->isFuzzy()) { + primarySupptIt->extendSuppAlignment( + secondarySuppIt->getPos(), + secondarySuppIt->getExtendedPos()); + } + foundMatch = true; + for (auto index : + secondarySuppIt->getSupportingIndicesSecondary()) { + lowMapqHardSupportWhitelistIndices.push_back(index); + } + secondarySuppIt->setToRemove(true); + primarySupptIt->addSecondarySupportIndices( + secondarySuppIt->getSupportingIndicesSecondary()); } - foundMatch = true; - secondarySuppIt->setToRemove(true); - primarySupptIt->addSecondarySupportIndices( - secondarySuppIt->getSupportingIndicesSecondary()); } - } - for (auto secondarySuppIt = saHardTmpLowQual.begin(); - secondarySuppIt != saHardTmpLowQual.end(); ++secondarySuppIt) { - if (primarySupptIt->saCloseness(*secondarySuppIt, 100)) { - if (primarySupptIt->isFuzzy() && - !secondarySuppIt->isFuzzy()) { - primarySupptIt->removeFuzziness(*secondarySuppIt); - } else if (primarySupptIt->isFuzzy() && - secondarySuppIt->isFuzzy()) { - primarySupptIt->extendSuppAlignment( - secondarySuppIt->getPos(), - secondarySuppIt->getExtendedPos()); - } - foundMatch = true; - for (auto index : - secondarySuppIt->getSupportingIndicesSecondary()) { - lowMapqHardSupportWhitelistIndices.push_back(index); - } - secondarySuppIt->setToRemove(true); - primarySupptIt->addSecondarySupportIndices( - secondarySuppIt->getSupportingIndicesSecondary()); + if (foundMatch) { + doubleSidedMatches.push_back(*primarySupptIt); + primarySupptIt->setToRemove(true); } } - if (foundMatch) { - doubleSidedMatches.push_back(*primarySupptIt); - primarySupptIt->setToRemove(true); + cleanUpVector(supplementsPrimary); + cleanUpVector(supplementsSecondary); + cleanUpVector(saHardTmpLowQual); + supplementsSecondary.insert(supplementsSecondary.end(), + saHardTmpLowQual.begin(), + saHardTmpLowQual.end()); + sort(lowMapqHardSupportWhitelistIndices.begin(), + lowMapqHardSupportWhitelistIndices.end()); + auto whitelistSize = + distance(lowMapqHardSupportWhitelistIndices.begin(), + unique(lowMapqHardSupportWhitelistIndices.begin(), + lowMapqHardSupportWhitelistIndices.end())); + unpairedBreaksHard += whitelistSize; + lowQualBreaksHard -= whitelistSize; + } + auto maxMapq = 0; + for (const auto &sa : doubleSidedMatches) { + if (sa.getMapq() > maxMapq) { + maxMapq = sa.getMapq(); } } - cleanUpVector(supplementsPrimary); - cleanUpVector(supplementsSecondary); - cleanUpVector(saHardTmpLowQual); - supplementsSecondary.insert(supplementsSecondary.end(), - saHardTmpLowQual.begin(), - saHardTmpLowQual.end()); - sort(lowMapqHardSupportWhitelistIndices.begin(), - lowMapqHardSupportWhitelistIndices.end()); - auto whitelistSize = - distance(lowMapqHardSupportWhitelistIndices.begin(), - unique(lowMapqHardSupportWhitelistIndices.begin(), - lowMapqHardSupportWhitelistIndices.end())); - unpairedBreaksHard += whitelistSize; - lowQualBreaksHard -= whitelistSize; - } - auto maxMapq = 0; - for (const auto &sa : doubleSidedMatches) { - if (sa.getMapq() > maxMapq) { - maxMapq = sa.getMapq(); - } - } - for (const auto &sa : supplementsPrimary) { - if (sa.getMapq() > maxMapq) { - maxMapq = sa.getMapq(); + for (const auto &sa : supplementsPrimary) { + if (sa.getMapq() > maxMapq) { + maxMapq = sa.getMapq(); + } } - } - for (auto &sa : supplementsPrimary) { - if (sa.getMapq() > 0) { - if (sa.getMapq() < 13 && sa.getMapq() < maxMapq) { - sa.setToRemove(true); + for (auto &sa : supplementsPrimary) { + if (sa.getMapq() > 0) { + if (sa.getMapq() < 13 && sa.getMapq() < maxMapq) { + sa.setToRemove(true); + } } } + cleanUpVector(supplementsPrimary); + for (auto &sa : supplementsPrimary) { + sa.finalizeSupportingIndices(); + } + vector uniqueDoubleSupportPrimaryIndices{}; + vector uniqueDoubleSupportSecondaryIndices{}; + for (auto &sa : doubleSidedMatches) { + sa.finalizeSupportingIndices(); + uniqueDoubleSupportPrimaryIndices.insert( + uniqueDoubleSupportPrimaryIndices.end(), + sa.getSupportingIndices().cbegin(), + sa.getSupportingIndices().cend()); + uniqueDoubleSupportSecondaryIndices.insert( + uniqueDoubleSupportSecondaryIndices.end(), + sa.getSupportingIndicesSecondary().cbegin(), + sa.getSupportingIndicesSecondary().cend()); + } + sort(uniqueDoubleSupportPrimaryIndices.begin(), + uniqueDoubleSupportPrimaryIndices.end()); + sort(uniqueDoubleSupportSecondaryIndices.begin(), + uniqueDoubleSupportSecondaryIndices.end()); + auto priCompensation = + distance(uniqueDoubleSupportPrimaryIndices.begin(), + unique(uniqueDoubleSupportPrimaryIndices.begin(), + uniqueDoubleSupportPrimaryIndices.end())); + auto secCompensation = + distance(uniqueDoubleSupportSecondaryIndices.begin(), + unique(uniqueDoubleSupportSecondaryIndices.begin(), + uniqueDoubleSupportSecondaryIndices.end())); + unpairedBreaksSoft -= priCompensation; + unpairedBreaksHard -= secCompensation; + pairedBreaksSoft += priCompensation; + pairedBreaksHard += secCompensation; } - cleanUpVector(supplementsPrimary); - for (auto &sa : supplementsPrimary) { - sa.finalizeSupportingIndices(); - } - vector uniqueDoubleSupportPrimaryIndices{}; - vector uniqueDoubleSupportSecondaryIndices{}; - for (auto &sa : doubleSidedMatches) { - sa.finalizeSupportingIndices(); - uniqueDoubleSupportPrimaryIndices.insert( - uniqueDoubleSupportPrimaryIndices.end(), - sa.getSupportingIndices().cbegin(), - sa.getSupportingIndices().cend()); - uniqueDoubleSupportSecondaryIndices.insert( - uniqueDoubleSupportSecondaryIndices.end(), - sa.getSupportingIndicesSecondary().cbegin(), - sa.getSupportingIndicesSecondary().cend()); - } - sort(uniqueDoubleSupportPrimaryIndices.begin(), - uniqueDoubleSupportPrimaryIndices.end()); - sort(uniqueDoubleSupportSecondaryIndices.begin(), - uniqueDoubleSupportSecondaryIndices.end()); - auto priCompensation = - distance(uniqueDoubleSupportPrimaryIndices.begin(), - unique(uniqueDoubleSupportPrimaryIndices.begin(), - uniqueDoubleSupportPrimaryIndices.end())); - auto secCompensation = - distance(uniqueDoubleSupportSecondaryIndices.begin(), - unique(uniqueDoubleSupportSecondaryIndices.begin(), - uniqueDoubleSupportSecondaryIndices.end())); - unpairedBreaksSoft -= priCompensation; - unpairedBreaksHard -= secCompensation; - pairedBreaksSoft += priCompensation; - pairedBreaksHard += secCompensation; -} -void -Breakpoint::collectMateSupport() { - sort(poolLeft.begin(), poolLeft.end()); - sort(poolRight.begin(), poolRight.end()); - compressMatePool(poolLeft); - compressMatePool(poolRight); - auto leftDiscordantsTotal = 0, rightDiscordantsTotal = 0; - for (const auto &mateInfo : poolLeft) { - leftDiscordantsTotal += mateInfo.matePower; - } - for (const auto &mateInfo : poolRight) { - rightDiscordantsTotal += mateInfo.matePower; - } - auto leftSideExpectedErrors = 0.0; - auto rightSideExpectedErrors = 0.0; - if (PROPERPAIRCOMPENSATIONMODE) { - leftSideExpectedErrors = - leftSideDiscordantCandidates * IMPROPERPAIRRATIO; - rightSideExpectedErrors = - rightSideDiscordantCandidates * IMPROPERPAIRRATIO; - leftDiscordantsTotal = - max(0, static_cast( - round(leftDiscordantsTotal - leftSideExpectedErrors))); - rightDiscordantsTotal = - max(0, static_cast( - round(rightDiscordantsTotal - rightSideExpectedErrors))); - leftSideExpectedErrors *= 0.5; - rightSideExpectedErrors *= 0.5; - } - for (auto &sa : doubleSidedMatches) { - if (sa.isDistant()) { - if (sa.isEncounteredM()) { - sa.setExpectedDiscordants(leftDiscordantsTotal); - collectMateSupportHelper(sa, poolLeft, poolLowQualLeft); - } else { - sa.setExpectedDiscordants(rightDiscordantsTotal); - collectMateSupportHelper(sa, poolRight, poolLowQualRight); + void + Breakpoint::collectMateSupport() { + sort(poolLeft.begin(), poolLeft.end()); + sort(poolRight.begin(), poolRight.end()); + compressMatePool(poolLeft); + compressMatePool(poolRight); + auto leftDiscordantsTotal = 0, rightDiscordantsTotal = 0; + for (const auto &mateInfo : poolLeft) { + leftDiscordantsTotal += mateInfo.matePower; + } + for (const auto &mateInfo : poolRight) { + rightDiscordantsTotal += mateInfo.matePower; + } + auto leftSideExpectedErrors = 0.0; + auto rightSideExpectedErrors = 0.0; + if (PROPERPAIRCOMPENSATIONMODE) { + leftSideExpectedErrors = + leftSideDiscordantCandidates * IMPROPERPAIRRATIO; + rightSideExpectedErrors = + rightSideDiscordantCandidates * IMPROPERPAIRRATIO; + leftDiscordantsTotal = + max(0, static_cast( + round(leftDiscordantsTotal - leftSideExpectedErrors))); + rightDiscordantsTotal = + max(0, static_cast( + round(rightDiscordantsTotal - rightSideExpectedErrors))); + leftSideExpectedErrors *= 0.5; + rightSideExpectedErrors *= 0.5; + } + for (auto &sa : doubleSidedMatches) { + if (sa.isDistant()) { + if (sa.isEncounteredM()) { + sa.setExpectedDiscordants(leftDiscordantsTotal); + collectMateSupportHelper(sa, poolLeft, poolLowQualLeft); + } else { + sa.setExpectedDiscordants(rightDiscordantsTotal); + collectMateSupportHelper(sa, poolRight, poolLowQualRight); + } } } - } - for (auto &sa : supplementsPrimary) { - if (sa.isDistant()) { - if (sa.isEncounteredM()) { - sa.setExpectedDiscordants(leftDiscordantsTotal); - collectMateSupportHelper(sa, poolLeft, poolLowQualLeft); - } else { - sa.setExpectedDiscordants(rightDiscordantsTotal); - collectMateSupportHelper(sa, poolRight, poolLowQualRight); + for (auto &sa : supplementsPrimary) { + if (sa.isDistant()) { + if (sa.isEncounteredM()) { + sa.setExpectedDiscordants(leftDiscordantsTotal); + collectMateSupportHelper(sa, poolLeft, poolLowQualLeft); + } else { + sa.setExpectedDiscordants(rightDiscordantsTotal); + collectMateSupportHelper(sa, poolRight, poolLowQualRight); + } + } else if (sa.getSupport() < BPSUPPORTTHRESHOLD) { + sa.setToRemove(true); } - } else if (sa.getSupport() < BPSUPPORTTHRESHOLD) { - sa.setToRemove(true); } - } - for (auto &sa : supplementsPrimary) { - if (sa.getMapq() == 0 && sa.getMateSupport() == 0 && - sa.getDistinctReads() < 2 * BPSUPPORTTHRESHOLD) { - sa.setToRemove(true); + for (auto &sa : supplementsPrimary) { + if (sa.getMapq() == 0 && sa.getMateSupport() == 0 && + sa.getDistinctReads() < 2 * BPSUPPORTTHRESHOLD) { + sa.setToRemove(true); + } } - } - vector candidateSupplementsSecondary{}; - sort(supplementsSecondary.begin(), supplementsSecondary.end(), - [](const SuppAlignment &a, const SuppAlignment &b) { - return a.getMapq() < b.getMapq(); - }); - while (!supplementsSecondary.empty()) { - auto foundMatch = false; - for (auto &sa : candidateSupplementsSecondary) { - if (sa.saCloseness(supplementsSecondary.back(), 100)) { - if (sa.isFuzzy() && !supplementsSecondary.back().isFuzzy()) { - sa.removeFuzziness(supplementsSecondary.back()); - } else if (sa.isFuzzy() && - supplementsSecondary.back().isFuzzy()) { - sa.extendSuppAlignment( - supplementsSecondary.back().getPos(), - supplementsSecondary.back().getExtendedPos()); - } - if (supplementsSecondary.back().getMapq() > sa.getMapq()) { - sa.setMapq(supplementsSecondary.back().getMapq()); - } - if (sa.isNullMapqSource() && - !supplementsSecondary.back().isNullMapqSource()) { - sa.setNullMapqSource(false); - } - for (auto index : supplementsSecondary.back() - .getSupportingIndicesSecondary()) { - sa.addSecondarySupportIndices(index); + vector candidateSupplementsSecondary{}; + sort(supplementsSecondary.begin(), supplementsSecondary.end(), + [](const SuppAlignment &a, const SuppAlignment &b) { + return a.getMapq() < b.getMapq(); + }); + while (!supplementsSecondary.empty()) { + auto foundMatch = false; + for (auto &sa : candidateSupplementsSecondary) { + if (sa.saCloseness(supplementsSecondary.back(), 100)) { + if (sa.isFuzzy() && !supplementsSecondary.back().isFuzzy()) { + sa.removeFuzziness(supplementsSecondary.back()); + } else if (sa.isFuzzy() && + supplementsSecondary.back().isFuzzy()) { + sa.extendSuppAlignment( + supplementsSecondary.back().getPos(), + supplementsSecondary.back().getExtendedPos()); + } + if (supplementsSecondary.back().getMapq() > sa.getMapq()) { + sa.setMapq(supplementsSecondary.back().getMapq()); + } + if (sa.isNullMapqSource() && + !supplementsSecondary.back().isNullMapqSource()) { + sa.setNullMapqSource(false); + } + for (auto index : supplementsSecondary.back() + .getSupportingIndicesSecondary()) { + sa.addSecondarySupportIndices(index); + } + foundMatch = true; + break; } - foundMatch = true; - break; } + if (!foundMatch) { + candidateSupplementsSecondary.push_back( + supplementsSecondary.back()); + } + supplementsSecondary.pop_back(); } - if (!foundMatch) { - candidateSupplementsSecondary.push_back( - supplementsSecondary.back()); + for (auto &sa : candidateSupplementsSecondary) { + sa.finalizeSupportingIndices(); } - supplementsSecondary.pop_back(); - } - for (auto &sa : candidateSupplementsSecondary) { - sa.finalizeSupportingIndices(); - } - vector originIndices{}; - for (auto &sa : candidateSupplementsSecondary) { - if (sa.isDistant()) { - if (sa.isEncounteredM()) { - sa.setExpectedDiscordants(leftDiscordantsTotal); - collectMateSupportHelper(sa, poolLeft, poolLowQualLeft); - } else { - sa.setExpectedDiscordants(rightDiscordantsTotal); - collectMateSupportHelper(sa, poolRight, poolLowQualRight); - } - if (sa.getMateSupport() > 0) { - doubleSidedMatches.push_back(sa); - } else { - if (sa.isLowMapqSource() || sa.isNullMapqSource() || - sa.getMapq() < 13) { - for (auto index : sa.getSupportingIndicesSecondary()) { - originIndices.push_back(index); + vector originIndices{}; + for (auto &sa : candidateSupplementsSecondary) { + if (sa.isDistant()) { + if (sa.isEncounteredM()) { + sa.setExpectedDiscordants(leftDiscordantsTotal); + collectMateSupportHelper(sa, poolLeft, poolLowQualLeft); + } else { + sa.setExpectedDiscordants(rightDiscordantsTotal); + collectMateSupportHelper(sa, poolRight, poolLowQualRight); + } + if (sa.getMateSupport() > 0) { + doubleSidedMatches.push_back(sa); + } else { + if (sa.isLowMapqSource() || sa.isNullMapqSource() || + sa.getMapq() < 13) { + for (auto index : sa.getSupportingIndicesSecondary()) { + originIndices.push_back(index); + } } } } } - } - sort(originIndices.begin(), originIndices.end()); - int uniqueCount = unique(originIndices.begin(), originIndices.end()) - - originIndices.begin(); - uniqueCount = min(lowQualBreaksHard, uniqueCount); - lowQualBreaksHard -= uniqueCount; - lowQualBreaksSoft += uniqueCount; + sort(originIndices.begin(), originIndices.end()); + int uniqueCount = unique(originIndices.begin(), originIndices.end()) - + originIndices.begin(); + uniqueCount = min(lowQualBreaksHard, uniqueCount); + lowQualBreaksHard -= uniqueCount; + lowQualBreaksSoft += uniqueCount; - for (const auto &mateInfo : poolLeft) { - if (!mateInfo.saSupporter && mateInfo.evidenceLevel == 3 && - mateInfo.matePower / (0.0 + leftDiscordantsTotal) >= 0.33 && - (pos - mateInfo.readEndPos) < DEFAULTREADLENGTH / 2) { - supplementsPrimary.emplace_back( - mateInfo.mateChrIndex, mateInfo.mateStartPos, - mateInfo.matePower, leftDiscordantsTotal, true, - mateInfo.inversionSupport > mateInfo.straightSupport, - mateInfo.mateEndPos, false, false, false, -1); + for (const auto &mateInfo : poolLeft) { + if (!mateInfo.saSupporter && mateInfo.evidenceLevel == 3 && + mateInfo.matePower / (0.0 + leftDiscordantsTotal) >= 0.33 && + (pos - mateInfo.readEndPos) < DEFAULTREADLENGTH / 2) { + supplementsPrimary.emplace_back( + mateInfo.mateChrIndex, mateInfo.mateStartPos, + mateInfo.matePower, leftDiscordantsTotal, true, + mateInfo.inversionSupport > mateInfo.straightSupport, + mateInfo.mateEndPos, false, false, false, -1); + } } - } - for (const auto &mateInfo : poolRight) { - if (!mateInfo.saSupporter && mateInfo.evidenceLevel == 3 && - mateInfo.matePower / (0.0 + rightDiscordantsTotal) >= 0.33 && - (mateInfo.readStartPos - pos) < DEFAULTREADLENGTH / 2) { - supplementsPrimary.emplace_back( - mateInfo.mateChrIndex, mateInfo.mateStartPos, - mateInfo.matePower, rightDiscordantsTotal, false, - mateInfo.inversionSupport > mateInfo.straightSupport, - mateInfo.mateEndPos, false, false, false, -1); + for (const auto &mateInfo : poolRight) { + if (!mateInfo.saSupporter && mateInfo.evidenceLevel == 3 && + mateInfo.matePower / (0.0 + rightDiscordantsTotal) >= 0.33 && + (mateInfo.readStartPos - pos) < DEFAULTREADLENGTH / 2) { + supplementsPrimary.emplace_back( + mateInfo.mateChrIndex, mateInfo.mateStartPos, + mateInfo.matePower, rightDiscordantsTotal, false, + mateInfo.inversionSupport > mateInfo.straightSupport, + mateInfo.mateEndPos, false, false, false, -1); + } } - } - for (auto &sa : doubleSidedMatches) { - if (sa.isFuzzy() && sa.getMateSupport() == 0) { - sa.setToRemove(true); + for (auto &sa : doubleSidedMatches) { + if (sa.isFuzzy() && sa.getMateSupport() == 0) { + sa.setToRemove(true); + } } - } - cleanUpVector(doubleSidedMatches); + cleanUpVector(doubleSidedMatches); - for (auto &sa : supplementsPrimary) { - if (sa.isFuzzy() && sa.getMateSupport() == 0) { - sa.setToRemove(true); + for (auto &sa : supplementsPrimary) { + if (sa.isFuzzy() && sa.getMateSupport() == 0) { + sa.setToRemove(true); + } } - } - cleanUpVector(supplementsPrimary); - for (auto &sa : doubleSidedMatches) { - for (auto &sa2 : supplementsPrimary) { - if (sa.saCloseness(sa2, 5)) { - sa.mergeSa(sa2); - sa2.setToRemove(true); + cleanUpVector(supplementsPrimary); + for (auto &sa : doubleSidedMatches) { + for (auto &sa2 : supplementsPrimary) { + if (sa.saCloseness(sa2, 5)) { + sa.mergeSa(sa2); + sa2.setToRemove(true); + } } } - } - cleanUpVector(supplementsPrimary); - for (auto &sa : doubleSidedMatches) { - if (!(sa.getSupport() > 0 && sa.getSecondarySupport() > 0) && - sa.getSupport() + sa.getSecondarySupport() + sa.getMateSupport() < - BPSUPPORTTHRESHOLD) { - sa.setToRemove(true); - } else { - if (sa.isDistant() && PROPERPAIRCOMPENSATIONMODE) { - if (sa.isEncounteredM()) { - if (sa.getMateSupport() < leftSideExpectedErrors) { - sa.setProperPairErrorProne(true); + cleanUpVector(supplementsPrimary); + for (auto &sa : doubleSidedMatches) { + if (!(sa.getSupport() > 0 && sa.getSecondarySupport() > 0) && + sa.getSupport() + sa.getSecondarySupport() + sa.getMateSupport() < + BPSUPPORTTHRESHOLD) { + sa.setToRemove(true); + } else { + if (sa.isDistant() && PROPERPAIRCOMPENSATIONMODE) { + if (sa.isEncounteredM()) { + if (sa.getMateSupport() < leftSideExpectedErrors) { + sa.setProperPairErrorProne(true); + } + } else { + if (sa.getMateSupport() < rightSideExpectedErrors) { + sa.setProperPairErrorProne(true); + } } - } else { - if (sa.getMateSupport() < rightSideExpectedErrors) { - sa.setProperPairErrorProne(true); + if (sa.getMateSupport() > sa.getExpectedDiscordants()) { + sa.setExpectedDiscordants(sa.getMateSupport()); } } - if (sa.getMateSupport() > sa.getExpectedDiscordants()) { - sa.setExpectedDiscordants(sa.getMateSupport()); - } } } - } - cleanUpVector(doubleSidedMatches); - for (auto &sa : supplementsPrimary) { - if (!(sa.getSupport() > 0 && sa.getSecondarySupport() > 0) && - sa.getSupport() + sa.getSecondarySupport() + sa.getMateSupport() < - BPSUPPORTTHRESHOLD) { - sa.setToRemove(true); - } else { - if (sa.isDistant() && PROPERPAIRCOMPENSATIONMODE) { - if (sa.isEncounteredM()) { - if (sa.getMateSupport() < leftSideExpectedErrors) { - sa.setProperPairErrorProne(true); + cleanUpVector(doubleSidedMatches); + for (auto &sa : supplementsPrimary) { + if (!(sa.getSupport() > 0 && sa.getSecondarySupport() > 0) && + sa.getSupport() + sa.getSecondarySupport() + sa.getMateSupport() < + BPSUPPORTTHRESHOLD) { + sa.setToRemove(true); + } else { + if (sa.isDistant() && PROPERPAIRCOMPENSATIONMODE) { + if (sa.isEncounteredM()) { + if (sa.getMateSupport() < leftSideExpectedErrors) { + sa.setProperPairErrorProne(true); + } + } else { + if (sa.getMateSupport() < rightSideExpectedErrors) { + sa.setProperPairErrorProne(true); + } } - } else { - if (sa.getMateSupport() < rightSideExpectedErrors) { - sa.setProperPairErrorProne(true); + if (sa.getMateSupport() > sa.getExpectedDiscordants()) { + sa.setExpectedDiscordants(sa.getMateSupport()); } } - if (sa.getMateSupport() > sa.getExpectedDiscordants()) { - sa.setExpectedDiscordants(sa.getMateSupport()); - } } } + cleanUpVector(supplementsPrimary); } - cleanUpVector(supplementsPrimary); -} -void -Breakpoint::compressMatePool(vector &discordantAlignmentsPool) { - if (discordantAlignmentsPool.empty()) - return; - auto lastIndex = 0; - for (auto i = 1; i < static_cast(discordantAlignmentsPool.size()); - ++i) { - if (discordantAlignmentsPool[lastIndex].mateChrIndex != - discordantAlignmentsPool[i].mateChrIndex || // - discordantAlignmentsPool[i].mateStartPos - - discordantAlignmentsPool[lastIndex].mateEndPos > - 3.5 * DEFAULTREADLENGTH) { - lastIndex = i; - } else { - discordantAlignmentsPool[lastIndex].mateEndPos = - max(discordantAlignmentsPool[lastIndex].mateEndPos, - discordantAlignmentsPool[i].mateEndPos); - discordantAlignmentsPool[lastIndex].mateStartPos = - min(discordantAlignmentsPool[lastIndex].mateStartPos, - discordantAlignmentsPool[i].mateStartPos); - ++discordantAlignmentsPool[lastIndex].matePower; - if (discordantAlignmentsPool[i].inverted) { - ++discordantAlignmentsPool[lastIndex].inversionSupport; + void + Breakpoint::compressMatePool(vector &discordantAlignmentsPool) { + if (discordantAlignmentsPool.empty()) + return; + auto lastIndex = 0; + for (auto i = 1; i < static_cast(discordantAlignmentsPool.size()); + ++i) { + if (discordantAlignmentsPool[lastIndex].mateChrIndex != + discordantAlignmentsPool[i].mateChrIndex || // + discordantAlignmentsPool[i].mateStartPos - + discordantAlignmentsPool[lastIndex].mateEndPos > + 3.5 * DEFAULTREADLENGTH) { + lastIndex = i; } else { - ++discordantAlignmentsPool[lastIndex].straightSupport; - } - if (abs(pos - discordantAlignmentsPool[i].readStartPos) <= - abs(pos - discordantAlignmentsPool[i].readEndPos)) { - // left side - if (abs(pos - discordantAlignmentsPool[lastIndex].readEndPos) > + discordantAlignmentsPool[lastIndex].mateEndPos = + max(discordantAlignmentsPool[lastIndex].mateEndPos, + discordantAlignmentsPool[i].mateEndPos); + discordantAlignmentsPool[lastIndex].mateStartPos = + min(discordantAlignmentsPool[lastIndex].mateStartPos, + discordantAlignmentsPool[i].mateStartPos); + ++discordantAlignmentsPool[lastIndex].matePower; + if (discordantAlignmentsPool[i].inverted) { + ++discordantAlignmentsPool[lastIndex].inversionSupport; + } else { + ++discordantAlignmentsPool[lastIndex].straightSupport; + } + if (abs(pos - discordantAlignmentsPool[i].readStartPos) <= abs(pos - discordantAlignmentsPool[i].readEndPos)) { - discordantAlignmentsPool[lastIndex].readStartPos = - discordantAlignmentsPool[i].readStartPos; - discordantAlignmentsPool[lastIndex].readEndPos = - discordantAlignmentsPool[i].readEndPos; + // left side + if (abs(pos - discordantAlignmentsPool[lastIndex].readEndPos) > + abs(pos - discordantAlignmentsPool[i].readEndPos)) { + discordantAlignmentsPool[lastIndex].readStartPos = + discordantAlignmentsPool[i].readStartPos; + discordantAlignmentsPool[lastIndex].readEndPos = + discordantAlignmentsPool[i].readEndPos; + } + } else { + // right side + if (abs(pos - + discordantAlignmentsPool[lastIndex].readStartPos) > + abs(pos - discordantAlignmentsPool[i].readStartPos)) { + discordantAlignmentsPool[lastIndex].readStartPos = + discordantAlignmentsPool[i].readStartPos; + discordantAlignmentsPool[lastIndex].readEndPos = + discordantAlignmentsPool[i].readEndPos; + } } - } else { - // right side - if (abs(pos - - discordantAlignmentsPool[lastIndex].readStartPos) > - abs(pos - discordantAlignmentsPool[i].readStartPos)) { - discordantAlignmentsPool[lastIndex].readStartPos = - discordantAlignmentsPool[i].readStartPos; - discordantAlignmentsPool[lastIndex].readEndPos = - discordantAlignmentsPool[i].readEndPos; + if ((discordantAlignmentsPool[lastIndex].source == 0 && + discordantAlignmentsPool[i].source == 1) || + (discordantAlignmentsPool[lastIndex].source == 1 && + discordantAlignmentsPool[i].source == 0)) { + discordantAlignmentsPool[lastIndex].evidenceLevel = 2; + discordantAlignmentsPool[lastIndex].source = 2; } + if (discordantAlignmentsPool[lastIndex].evidenceLevel != 3 && + discordantAlignmentsPool[i].evidenceLevel == 3) { + discordantAlignmentsPool[lastIndex].evidenceLevel = 3; + discordantAlignmentsPool[lastIndex].source = 2; + } + discordantAlignmentsPool[i].toRemove = true; } - if ((discordantAlignmentsPool[lastIndex].source == 0 && - discordantAlignmentsPool[i].source == 1) || - (discordantAlignmentsPool[lastIndex].source == 1 && - discordantAlignmentsPool[i].source == 0)) { - discordantAlignmentsPool[lastIndex].evidenceLevel = 2; - discordantAlignmentsPool[lastIndex].source = 2; - } - if (discordantAlignmentsPool[lastIndex].evidenceLevel != 3 && - discordantAlignmentsPool[i].evidenceLevel == 3) { - discordantAlignmentsPool[lastIndex].evidenceLevel = 3; - discordantAlignmentsPool[lastIndex].source = 2; - } - discordantAlignmentsPool[i].toRemove = true; } - } - for (auto &cluster : discordantAlignmentsPool) { - if (cluster.evidenceLevel == 1 && - cluster.matePower < BPSUPPORTTHRESHOLD) { - cluster.toRemove = true; + for (auto &cluster : discordantAlignmentsPool) { + if (cluster.evidenceLevel == 1 && + cluster.matePower < BPSUPPORTTHRESHOLD) { + cluster.toRemove = true; + } } + cleanUpVector(discordantAlignmentsPool); } - cleanUpVector(discordantAlignmentsPool); -} -void -Breakpoint::fillMatePool( - const deque &discordantAlignmentsPool, - const deque &discordantLowQualAlignmentsPool, - const deque &discordantAlignmentCandidatesPool) { - poolLeft.reserve(discordantAlignmentsPool.size()); - poolRight.reserve(discordantAlignmentsPool.size()); - { - auto i = 0u; - for (; i < discordantAlignmentsPool.size(); ++i) { - if (discordantAlignmentsPool[i].readStartPos >= pos) { - break; - } else { - if (discordantAlignmentsPool[i].readEndPos <= pos) { - poolLeft.push_back(discordantAlignmentsPool[i]); + void + Breakpoint::fillMatePool( + const deque &discordantAlignmentsPool, + const deque &discordantLowQualAlignmentsPool, + const deque &discordantAlignmentCandidatesPool) { + poolLeft.reserve(discordantAlignmentsPool.size()); + poolRight.reserve(discordantAlignmentsPool.size()); + { + auto i = 0u; + for (; i < discordantAlignmentsPool.size(); ++i) { + if (discordantAlignmentsPool[i].readStartPos >= pos) { + break; } else { - poolLeft.push_back(discordantAlignmentsPool[i]); - poolRight.push_back(discordantAlignmentsPool[i]); + if (discordantAlignmentsPool[i].readEndPos <= pos) { + poolLeft.push_back(discordantAlignmentsPool[i]); + } else { + poolLeft.push_back(discordantAlignmentsPool[i]); + poolRight.push_back(discordantAlignmentsPool[i]); + } } } + for (; i < discordantAlignmentsPool.size(); ++i) { + poolRight.push_back(discordantAlignmentsPool[i]); + } } - for (; i < discordantAlignmentsPool.size(); ++i) { - poolRight.push_back(discordantAlignmentsPool[i]); - } - } - if (PROPERPAIRCOMPENSATIONMODE) { - auto i = 0u; - for (; i < discordantAlignmentCandidatesPool.size(); ++i) { - if (discordantAlignmentCandidatesPool[i].readStartPos >= pos) { - break; - } else { - if (discordantAlignmentCandidatesPool[i].readEndPos <= pos) { - ++leftSideDiscordantCandidates; + if (PROPERPAIRCOMPENSATIONMODE) { + auto i = 0u; + for (; i < discordantAlignmentCandidatesPool.size(); ++i) { + if (discordantAlignmentCandidatesPool[i].readStartPos >= pos) { + break; } else { - ++leftSideDiscordantCandidates; - ++rightSideDiscordantCandidates; + if (discordantAlignmentCandidatesPool[i].readEndPos <= pos) { + ++leftSideDiscordantCandidates; + } else { + ++leftSideDiscordantCandidates; + ++rightSideDiscordantCandidates; + } } } - } - for (; i < discordantAlignmentCandidatesPool.size(); ++i) { - ++rightSideDiscordantCandidates; - } - } - poolLowQualLeft.reserve(discordantLowQualAlignmentsPool.size()); - poolLowQualRight.reserve(discordantLowQualAlignmentsPool.size()); - { - auto i = 0u; - for (; i < discordantLowQualAlignmentsPool.size(); ++i) { - if (discordantLowQualAlignmentsPool[i].readStartPos < - pos - DISCORDANTLOWQUALLEFTRANGE) { - continue; + for (; i < discordantAlignmentCandidatesPool.size(); ++i) { + ++rightSideDiscordantCandidates; } - if (discordantLowQualAlignmentsPool[i].readStartPos >= pos) { - break; - } else { - if (discordantLowQualAlignmentsPool[i].readEndPos <= pos) { - poolLowQualLeft.push_back( - discordantLowQualAlignmentsPool[i]); + } + poolLowQualLeft.reserve(discordantLowQualAlignmentsPool.size()); + poolLowQualRight.reserve(discordantLowQualAlignmentsPool.size()); + { + auto i = 0u; + for (; i < discordantLowQualAlignmentsPool.size(); ++i) { + if (discordantLowQualAlignmentsPool[i].readStartPos < + pos - DISCORDANTLOWQUALLEFTRANGE) { + continue; + } + if (discordantLowQualAlignmentsPool[i].readStartPos >= pos) { + break; } else { - poolLowQualLeft.push_back( - discordantLowQualAlignmentsPool[i]); - poolLowQualRight.push_back( - discordantLowQualAlignmentsPool[i]); + if (discordantLowQualAlignmentsPool[i].readEndPos <= pos) { + poolLowQualLeft.push_back( + discordantLowQualAlignmentsPool[i]); + } else { + poolLowQualLeft.push_back( + discordantLowQualAlignmentsPool[i]); + poolLowQualRight.push_back( + discordantLowQualAlignmentsPool[i]); + } } } - } - for (; i < discordantLowQualAlignmentsPool.size(); ++i) { - if (discordantLowQualAlignmentsPool[i].readStartPos > - pos + DISCORDANTLOWQUALRIGHTRANGE) { - break; + for (; i < discordantLowQualAlignmentsPool.size(); ++i) { + if (discordantLowQualAlignmentsPool[i].readStartPos > + pos + DISCORDANTLOWQUALRIGHTRANGE) { + break; + } + poolLowQualRight.push_back(discordantLowQualAlignmentsPool[i]); } - poolLowQualRight.push_back(discordantLowQualAlignmentsPool[i]); } } -} -void -Breakpoint::collectMateSupportHelper( - SuppAlignment &sa, vector &discordantAlignmentsPool, - vector &discordantLowQualAlignmentsPool) { - auto maxEvidenceLevel = 0; - for (auto &mateInfo : discordantAlignmentsPool) { - if (mateInfo.suppAlignmentFuzzyMatch(sa)) { - if (!mateInfo.saSupporter) { - mateSupport += mateInfo.matePower; - mateInfo.saSupporter = true; - } - sa.incrementMateSupport(mateInfo.matePower); - if (sa.isFuzzy()) { - sa.extendSuppAlignment(mateInfo.mateStartPos, - mateInfo.mateEndPos); - } - if (mateInfo.evidenceLevel > maxEvidenceLevel) { - maxEvidenceLevel = mateInfo.evidenceLevel; - } - } - } - int lowQualSupports{0}; - for (auto &mateInfo : discordantLowQualAlignmentsPool) { - if (mateInfo.suppAlignmentFuzzyMatch(sa)) { - if (!mateInfo.saSupporter) { - mateSupport += 1; - mateInfo.saSupporter = true; - } - sa.incrementMateSupport(1); - ++lowQualSupports; - auto bpPosMatch = false; - for (const auto bpPos : mateInfo.bpLocs) { - if (bpPos == pos) { - bpPosMatch = true; - break; + void + Breakpoint::collectMateSupportHelper( + SuppAlignment &sa, vector &discordantAlignmentsPool, + vector &discordantLowQualAlignmentsPool) { + auto maxEvidenceLevel = 0; + for (auto &mateInfo : discordantAlignmentsPool) { + if (mateInfo.suppAlignmentFuzzyMatch(sa)) { + if (!mateInfo.saSupporter) { + mateSupport += mateInfo.matePower; + mateInfo.saSupporter = true; + } + sa.incrementMateSupport(mateInfo.matePower); + if (sa.isFuzzy()) { + sa.extendSuppAlignment(mateInfo.mateStartPos, + mateInfo.mateEndPos); } - } - if (!bpPosMatch) { if (mateInfo.evidenceLevel > maxEvidenceLevel) { maxEvidenceLevel = mateInfo.evidenceLevel; } } } - } - auto lowQualDiscordantSupports = - lowQualSupports + - min(lowQualSupports, - static_cast(discordantLowQualAlignmentsPool.size()) - - lowQualSupports); - sa.setExpectedDiscordants(sa.getExpectedDiscordants() + - lowQualDiscordantSupports); - if (sa.getMateSupport() == 0) { - if (sa.getSecondarySupport() == 0 && - sa.getSupport() < BPSUPPORTTHRESHOLD) { - sa.setToRemove(true); - } else { - sa.setSuspicious(true); - } - } else { - if (maxEvidenceLevel < 3) { - sa.setSemiSuspicious(true); - if (!((0.0 + sa.getMateSupport()) / (sa.getExpectedDiscordants()) > - 0.33)) { - if (sa.getSecondarySupport() == 0 && - sa.getSupport() < BPSUPPORTTHRESHOLD) { - sa.setToRemove(true); + int lowQualSupports{0}; + for (auto &mateInfo : discordantLowQualAlignmentsPool) { + if (mateInfo.suppAlignmentFuzzyMatch(sa)) { + if (!mateInfo.saSupporter) { + mateSupport += 1; + mateInfo.saSupporter = true; } + sa.incrementMateSupport(1); + ++lowQualSupports; + auto bpPosMatch = false; + for (const auto bpPos : mateInfo.bpLocs) { + if (bpPos == pos) { + bpPosMatch = true; + break; + } + } + if (!bpPosMatch) { + if (mateInfo.evidenceLevel > maxEvidenceLevel) { + maxEvidenceLevel = mateInfo.evidenceLevel; + } + } + } + } + auto lowQualDiscordantSupports = + lowQualSupports + + min(lowQualSupports, + static_cast(discordantLowQualAlignmentsPool.size()) - + lowQualSupports); + sa.setExpectedDiscordants(sa.getExpectedDiscordants() + + lowQualDiscordantSupports); + if (sa.getMateSupport() == 0) { + if (sa.getSecondarySupport() == 0 && + sa.getSupport() < BPSUPPORTTHRESHOLD) { + sa.setToRemove(true); + } else { + sa.setSuspicious(true); } } else { - sa.setSemiSuspicious(false); + if (maxEvidenceLevel < 3) { + sa.setSemiSuspicious(true); + if (!((0.0 + sa.getMateSupport()) / (sa.getExpectedDiscordants()) > + 0.33)) { + if (sa.getSecondarySupport() == 0 && + sa.getSupport() < BPSUPPORTTHRESHOLD) { + sa.setToRemove(true); + } + } + } else { + sa.setSemiSuspicious(false); + } } } -} -Breakpoint::Breakpoint(const string &bpIn, bool ignoreOverhang) - : covFinalized{true}, - missingInfoBp{false}, - chrIndex{0}, - pos{0}, - normalSpans{0}, - lowQualSpansSoft{0}, - lowQualSpansHard{0}, - unpairedBreaksSoft{0}, - unpairedBreaksHard{0}, - breaksShortIndel{0}, - lowQualBreaksSoft{0}, - lowQualBreaksHard{0}, - repetitiveOverhangBreaks{0}, - pairedBreaksSoft{0}, - pairedBreaksHard{0}, - mateSupport{0}, - leftCoverage{0}, - rightCoverage{0}, - hitsInMref{0}, - germline{false} { - auto index = 0; - vector bpChunkPositions{}; - bpChunkPositions.reserve(7); - for (auto it = bpIn.cbegin(); it != bpIn.cend(); ++it) { - if (*it == '\t') { - bpChunkPositions.push_back(index); + Breakpoint::Breakpoint(const string &bpIn, bool ignoreOverhang) + : covFinalized{true}, + missingInfoBp{false}, + chrIndex{0}, + pos{0}, + normalSpans{0}, + lowQualSpansSoft{0}, + lowQualSpansHard{0}, + unpairedBreaksSoft{0}, + unpairedBreaksHard{0}, + breaksShortIndel{0}, + lowQualBreaksSoft{0}, + lowQualBreaksHard{0}, + repetitiveOverhangBreaks{0}, + pairedBreaksSoft{0}, + pairedBreaksHard{0}, + mateSupport{0}, + leftCoverage{0}, + rightCoverage{0}, + hitsInMref{0}, + germline{false} { + auto index = 0; + vector bpChunkPositions{}; + bpChunkPositions.reserve(7); + for (auto it = bpIn.cbegin(); it != bpIn.cend(); ++it) { + if (*it == '\t') { + bpChunkPositions.push_back(index); + } + ++index; } - ++index; - } - chrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex(bpIn.cbegin(), '\t'); + chrIndex = GlobalAppConfig::getInstance().getChrConverter().readChromosomeIndex(bpIn.cbegin(), '\t'); - for (auto i = bpChunkPositions[0] + 1; i < bpChunkPositions[1]; ++i) { - pos = pos * 10 + (bpIn[i] - '0'); - } - auto mode = 0; - for (auto i = bpChunkPositions[2] + 1; i < bpChunkPositions[3]; ++i) { - if (bpIn[i] == ',') { - ++mode; - } else { - switch (mode) { - case 0: - pairedBreaksSoft = 10 * pairedBreaksSoft + (bpIn[i] - '0'); - break; - case 1: - pairedBreaksHard = 10 * pairedBreaksHard + (bpIn[i] - '0'); - break; - case 2: - mateSupport = 10 * mateSupport + (bpIn[i] - '0'); - break; - case 3: - unpairedBreaksSoft = 10 * unpairedBreaksSoft + (bpIn[i] - '0'); - break; - case 4: - unpairedBreaksHard = 10 * unpairedBreaksHard + (bpIn[i] - '0'); - break; - case 5: - breaksShortIndel = 10 * breaksShortIndel + (bpIn[i] - '0'); - break; - case 6: - normalSpans = 10 * normalSpans + (bpIn[i] - '0'); - break; - case 7: - lowQualSpansSoft = 10 * lowQualSpansSoft + (bpIn[i] - '0'); - break; - case 8: - lowQualSpansHard = 10 * lowQualSpansHard + (bpIn[i] - '0'); - break; - case 9: - lowQualBreaksSoft = 10 * lowQualBreaksSoft + (bpIn[i] - '0'); - break; - case 10: - lowQualBreaksHard = 10 * lowQualBreaksHard + (bpIn[i] - '0'); - break; - case 11: - repetitiveOverhangBreaks = - 10 * repetitiveOverhangBreaks + (bpIn[i] - '0'); - break; - default: - break; + for (auto i = bpChunkPositions[0] + 1; i < bpChunkPositions[1]; ++i) { + pos = pos * 10 + (bpIn[i] - '0'); + } + auto mode = 0; + for (auto i = bpChunkPositions[2] + 1; i < bpChunkPositions[3]; ++i) { + if (bpIn[i] == ',') { + ++mode; + } else { + switch (mode) { + case 0: + pairedBreaksSoft = 10 * pairedBreaksSoft + (bpIn[i] - '0'); + break; + case 1: + pairedBreaksHard = 10 * pairedBreaksHard + (bpIn[i] - '0'); + break; + case 2: + mateSupport = 10 * mateSupport + (bpIn[i] - '0'); + break; + case 3: + unpairedBreaksSoft = 10 * unpairedBreaksSoft + (bpIn[i] - '0'); + break; + case 4: + unpairedBreaksHard = 10 * unpairedBreaksHard + (bpIn[i] - '0'); + break; + case 5: + breaksShortIndel = 10 * breaksShortIndel + (bpIn[i] - '0'); + break; + case 6: + normalSpans = 10 * normalSpans + (bpIn[i] - '0'); + break; + case 7: + lowQualSpansSoft = 10 * lowQualSpansSoft + (bpIn[i] - '0'); + break; + case 8: + lowQualSpansHard = 10 * lowQualSpansHard + (bpIn[i] - '0'); + break; + case 9: + lowQualBreaksSoft = 10 * lowQualBreaksSoft + (bpIn[i] - '0'); + break; + case 10: + lowQualBreaksHard = 10 * lowQualBreaksHard + (bpIn[i] - '0'); + break; + case 11: + repetitiveOverhangBreaks = + 10 * repetitiveOverhangBreaks + (bpIn[i] - '0'); + break; + default: + break; + } } } - } - mode = 0; - for (auto i = bpChunkPositions[3] + 1; i < bpChunkPositions[4]; ++i) { - if (bpIn[i] == ',') { - ++mode; - } else { - switch (mode) { - case 0: - leftCoverage = 10 * leftCoverage + (bpIn[i] - '0'); - break; - case 1: - rightCoverage = 10 * rightCoverage + (bpIn[i] - '0'); - break; - default: - break; + mode = 0; + for (auto i = bpChunkPositions[3] + 1; i < bpChunkPositions[4]; ++i) { + if (bpIn[i] == ',') { + ++mode; + } else { + switch (mode) { + case 0: + leftCoverage = 10 * leftCoverage + (bpIn[i] - '0'); + break; + case 1: + rightCoverage = 10 * rightCoverage + (bpIn[i] - '0'); + break; + default: + break; + } } } - } - auto shortClipTotal = normalSpans - min(leftCoverage, rightCoverage); - if (shortClipTotal > 0) { - normalSpans -= shortClipTotal; - if (pairedBreaksSoft > 0) { - pairedBreaksSoft += shortClipTotal; - } else { - unpairedBreaksSoft += shortClipTotal; + auto shortClipTotal = normalSpans - min(leftCoverage, rightCoverage); + if (shortClipTotal > 0) { + normalSpans -= shortClipTotal; + if (pairedBreaksSoft > 0) { + pairedBreaksSoft += shortClipTotal; + } else { + unpairedBreaksSoft += shortClipTotal; + } } - } - if (bpIn[bpChunkPositions[4] + 1] == '#') { - missingInfoBp = true; - } else { - if (bpIn[bpChunkPositions[4] + 1] != '.') { - string saStr{}; - for (auto i = bpChunkPositions[4] + 1; i < bpChunkPositions[5]; - ++i) { - if (bpIn[i] == ';') { - doubleSidedMatches.emplace_back(saStr); - saStr.clear(); - } else { - saStr.push_back(bpIn[i]); + if (bpIn[bpChunkPositions[4] + 1] == '#') { + missingInfoBp = true; + } else { + if (bpIn[bpChunkPositions[4] + 1] != '.') { + string saStr{}; + for (auto i = bpChunkPositions[4] + 1; i < bpChunkPositions[5]; + ++i) { + if (bpIn[i] == ';') { + doubleSidedMatches.emplace_back(saStr); + saStr.clear(); + } else { + saStr.push_back(bpIn[i]); + } } - } - SuppAlignment saTmp{saStr}; - if (saTmp.getChrIndex() < 1002) { - doubleSidedMatches.push_back(saTmp); - } - } - if (bpIn[bpChunkPositions[5] + 1] != '.') { - string saStr{}; - for (auto i = bpChunkPositions[5] + 1; i < bpChunkPositions[6]; - ++i) { - if (bpIn[i] == ';') { - supplementsPrimary.emplace_back(saStr); - saStr.clear(); - } else { - saStr.push_back(bpIn[i]); + SuppAlignment saTmp{saStr}; + if (saTmp.getChrIndex() < 1002) { + doubleSidedMatches.push_back(saTmp); } } - SuppAlignment saTmp{saStr}; - if (saTmp.getChrIndex() < 1002) { - supplementsPrimary.push_back(saTmp); + if (bpIn[bpChunkPositions[5] + 1] != '.') { + string saStr{}; + for (auto i = bpChunkPositions[5] + 1; i < bpChunkPositions[6]; + ++i) { + if (bpIn[i] == ';') { + supplementsPrimary.emplace_back(saStr); + saStr.clear(); + } else { + saStr.push_back(bpIn[i]); + } + } + SuppAlignment saTmp{saStr}; + if (saTmp.getChrIndex() < 1002) { + supplementsPrimary.push_back(saTmp); + } } - } - cleanUpVector(supplementsPrimary); - saHomologyClashSolver(); - if (!ignoreOverhang && bpIn[bpChunkPositions[6] + 1] != '.') { - string overhang{}; - for (auto i = bpChunkPositions[6] + 1; - i < static_cast(bpIn.length()); ++i) { - if (bpIn[i] == ';') { - consensusOverhangs.emplace_back(overhang); - overhang.clear(); - } else { - overhang.push_back(bpIn[i]); + cleanUpVector(supplementsPrimary); + saHomologyClashSolver(); + if (!ignoreOverhang && bpIn[bpChunkPositions[6] + 1] != '.') { + string overhang{}; + for (auto i = bpChunkPositions[6] + 1; + i < static_cast(bpIn.length()); ++i) { + if (bpIn[i] == ';') { + consensusOverhangs.emplace_back(overhang); + overhang.clear(); + } else { + overhang.push_back(bpIn[i]); + } } + consensusOverhangs.emplace_back(overhang); } - consensusOverhangs.emplace_back(overhang); } } -} -void -Breakpoint::saHomologyClashSolver() { - for (auto i = 0u; i < doubleSidedMatches.size(); ++i) { - if (!doubleSidedMatches[i].isDistant() || - doubleSidedMatches[i].getMateSupport() == 0) { - continue; - } - bool anyMatch{false}; - bool semiSuspiciousRescue{false}; - for (auto j = 0u; j < doubleSidedMatches.size(); ++j) { - if (j == i) { + void + Breakpoint::saHomologyClashSolver() { + for (auto i = 0u; i < doubleSidedMatches.size(); ++i) { + if (!doubleSidedMatches[i].isDistant() || + doubleSidedMatches[i].getMateSupport() == 0) { continue; } - if (doubleSidedMatches[i].saDistHomologyRescueCloseness( - doubleSidedMatches[j], 200000)) { - if (!semiSuspiciousRescue && - doubleSidedMatches[i].isSemiSuspicious() && - !doubleSidedMatches[j].isSemiSuspicious()) { - semiSuspiciousRescue = true; + bool anyMatch{false}; + bool semiSuspiciousRescue{false}; + for (auto j = 0u; j < doubleSidedMatches.size(); ++j) { + if (j == i) { + continue; } - anyMatch = true; - break; - } - } - if (!anyMatch) { - for (auto j = 0u; j < supplementsPrimary.size(); ++j) { if (doubleSidedMatches[i].saDistHomologyRescueCloseness( - supplementsPrimary[j], 200000)) { + doubleSidedMatches[j], 200000)) { if (!semiSuspiciousRescue && doubleSidedMatches[i].isSemiSuspicious() && - !supplementsPrimary[j].isSemiSuspicious()) { + !doubleSidedMatches[j].isSemiSuspicious()) { semiSuspiciousRescue = true; } anyMatch = true; break; } } - } - if (anyMatch) { - doubleSidedMatches[i].padMateSupportHomologyRescue(); - if (semiSuspiciousRescue) { - doubleSidedMatches[i].setSemiSuspicious(false); + if (!anyMatch) { + for (auto j = 0u; j < supplementsPrimary.size(); ++j) { + if (doubleSidedMatches[i].saDistHomologyRescueCloseness( + supplementsPrimary[j], 200000)) { + if (!semiSuspiciousRescue && + doubleSidedMatches[i].isSemiSuspicious() && + !supplementsPrimary[j].isSemiSuspicious()) { + semiSuspiciousRescue = true; + } + anyMatch = true; + break; + } + } + } + if (anyMatch) { + doubleSidedMatches[i].padMateSupportHomologyRescue(); + if (semiSuspiciousRescue) { + doubleSidedMatches[i].setSemiSuspicious(false); + } } } - } - for (auto i = 0u; i < supplementsPrimary.size(); ++i) { - if (!supplementsPrimary[i].isDistant() || - supplementsPrimary[i].getMateSupport() == 0) { - continue; - } - bool anyMatch{false}; - bool semiSuspiciousRescue{false}; - for (auto j = 0u; j < supplementsPrimary.size(); ++j) { - if (j == i) { + for (auto i = 0u; i < supplementsPrimary.size(); ++i) { + if (!supplementsPrimary[i].isDistant() || + supplementsPrimary[i].getMateSupport() == 0) { continue; } - if (supplementsPrimary[i].saDistHomologyRescueCloseness( - supplementsPrimary[j], 100000)) { - if (!semiSuspiciousRescue && - supplementsPrimary[i].isSemiSuspicious() && - !supplementsPrimary[j].isSemiSuspicious()) { - semiSuspiciousRescue = true; + bool anyMatch{false}; + bool semiSuspiciousRescue{false}; + for (auto j = 0u; j < supplementsPrimary.size(); ++j) { + if (j == i) { + continue; } - anyMatch = true; - break; - } - } - if (!anyMatch) { - for (auto j = 0u; j < doubleSidedMatches.size(); ++j) { if (supplementsPrimary[i].saDistHomologyRescueCloseness( - doubleSidedMatches[j], 100000)) { + supplementsPrimary[j], 100000)) { if (!semiSuspiciousRescue && supplementsPrimary[i].isSemiSuspicious() && - !doubleSidedMatches[j].isSemiSuspicious()) { + !supplementsPrimary[j].isSemiSuspicious()) { semiSuspiciousRescue = true; } anyMatch = true; break; } } - } - if (anyMatch) { - supplementsPrimary[i].padMateSupportHomologyRescue(); - if (semiSuspiciousRescue) { - supplementsPrimary[i].setSemiSuspicious(false); + if (!anyMatch) { + for (auto j = 0u; j < doubleSidedMatches.size(); ++j) { + if (supplementsPrimary[i].saDistHomologyRescueCloseness( + doubleSidedMatches[j], 100000)) { + if (!semiSuspiciousRescue && + supplementsPrimary[i].isSemiSuspicious() && + !doubleSidedMatches[j].isSemiSuspicious()) { + semiSuspiciousRescue = true; + } + anyMatch = true; + break; + } + } + } + if (anyMatch) { + supplementsPrimary[i].padMateSupportHomologyRescue(); + if (semiSuspiciousRescue) { + supplementsPrimary[i].setSemiSuspicious(false); + } } } } -} -SuppAlignment * -Breakpoint::searchFuzzySa(const SuppAlignment &fuzzySa) { - SuppAlignment *match = nullptr; - for (auto &saDouble : doubleSidedMatches) { - if (saDouble.saCloseness(fuzzySa, 1)) { - match = &saDouble; - return match; + SuppAlignment * + Breakpoint::searchFuzzySa(const SuppAlignment &fuzzySa) { + SuppAlignment *match = nullptr; + for (auto &saDouble : doubleSidedMatches) { + if (saDouble.saCloseness(fuzzySa, 1)) { + match = &saDouble; + return match; + } } - } - for (auto &saSingle : supplementsPrimary) { - if (saSingle.saCloseness(fuzzySa, 1)) { - match = &saSingle; - return match; + for (auto &saSingle : supplementsPrimary) { + if (saSingle.saCloseness(fuzzySa, 1)) { + match = &saSingle; + return match; + } } + return nullptr; } - return nullptr; -} } // namespace sophia - -/* namespace sophia */ diff --git a/src/BreakpointReduced.cpp b/src/BreakpointReduced.cpp index b1cfe84..c3516c7 100644 --- a/src/BreakpointReduced.cpp +++ b/src/BreakpointReduced.cpp @@ -32,180 +32,178 @@ namespace sophia { -using namespace std; + boost::format BreakpointReduced::doubleFormatter{"%.3f"}; + int BreakpointReduced::DEFAULTREADLENGTH{}; + double BreakpointReduced::CLONALITYSTRICTLOWTHRESHOLD{}; + double BreakpointReduced::ARTIFACTFREQHIGHTHRESHOLD{}; + string BreakpointReduced::PIDSINMREFSTR{}; -boost::format BreakpointReduced::doubleFormatter{"%.3f"}; -int BreakpointReduced::DEFAULTREADLENGTH{}; -double BreakpointReduced::CLONALITYSTRICTLOWTHRESHOLD{}; -double BreakpointReduced::ARTIFACTFREQHIGHTHRESHOLD{}; -string BreakpointReduced::PIDSINMREFSTR{}; - -sophia::BreakpointReduced::BreakpointReduced(const Breakpoint &tmpBp, - int lineIndexIn, - bool hasOverhangIn) - : hasOverhang{hasOverhangIn}, toRemove{false}, lineIndex{lineIndexIn}, - chrIndex{tmpBp.getChrIndex()}, pos{tmpBp.getPos()}, - normalSpans{tmpBp.getNormalSpans()}, - lowQualSpansSoft{tmpBp.getLowQualBreaksSoft()}, - lowQualSpansHard{tmpBp.getLowQualSpansHard()}, - unpairedBreaksSoft{tmpBp.getUnpairedBreaksSoft()}, - unpairedBreaksHard{tmpBp.getUnpairedBreaksHard()}, - breaksShortIndel{tmpBp.getBreaksShortIndel()}, - lowQualBreaksSoft{tmpBp.getLowQualBreaksSoft()}, - lowQualBreaksHard{tmpBp.getLowQualBreaksHard()}, - repetitiveOverhangBreaks{tmpBp.getRepetitiveOverhangBreaks()}, - pairedBreaksSoft{tmpBp.getPairedBreaksSoft()}, - pairedBreaksHard{tmpBp.getPairedBreaksHard()}, - mateSupport{tmpBp.getMateSupport()}, - leftCoverage{tmpBp.getLeftCoverage()}, - rightCoverage{tmpBp.getRightCoverage()}, - mrefHits{MrefMatch{-1, -1, 10000, {}, }}, - germlineInfo{GermlineMatch{0.0, 0.0, {}, }}, - suppAlignments{} { - for (const auto &sa : tmpBp.getDoubleSidedMatches()) { - if (sa.getChrIndex() < 1002) { - suppAlignments.emplace_back(sa); - } - } - for (const auto &sa : tmpBp.getSupplementsPrimary()) { - if (sa.getChrIndex() < 1002) { - suppAlignments.emplace_back(sa); + sophia::BreakpointReduced::BreakpointReduced(const Breakpoint &tmpBp, + int lineIndexIn, + bool hasOverhangIn) + : hasOverhang{hasOverhangIn}, toRemove{false}, lineIndex{lineIndexIn}, + chrIndex{tmpBp.getChrIndex()}, pos{tmpBp.getPos()}, + normalSpans{tmpBp.getNormalSpans()}, + lowQualSpansSoft{tmpBp.getLowQualBreaksSoft()}, + lowQualSpansHard{tmpBp.getLowQualSpansHard()}, + unpairedBreaksSoft{tmpBp.getUnpairedBreaksSoft()}, + unpairedBreaksHard{tmpBp.getUnpairedBreaksHard()}, + breaksShortIndel{tmpBp.getBreaksShortIndel()}, + lowQualBreaksSoft{tmpBp.getLowQualBreaksSoft()}, + lowQualBreaksHard{tmpBp.getLowQualBreaksHard()}, + repetitiveOverhangBreaks{tmpBp.getRepetitiveOverhangBreaks()}, + pairedBreaksSoft{tmpBp.getPairedBreaksSoft()}, + pairedBreaksHard{tmpBp.getPairedBreaksHard()}, + mateSupport{tmpBp.getMateSupport()}, + leftCoverage{tmpBp.getLeftCoverage()}, + rightCoverage{tmpBp.getRightCoverage()}, + mrefHits{MrefMatch{-1, -1, 10000, {}, }}, + germlineInfo{GermlineMatch{0.0, 0.0, {}, }}, + suppAlignments{} { + for (const auto &sa : tmpBp.getDoubleSidedMatches()) { + if (sa.getChrIndex() < 1002) { + suppAlignments.emplace_back(sa); + } } - } - complexRearrangementMateRatioRescue(true); - complexRearrangementMateRatioRescue(false); -} - -void -BreakpointReduced::complexRearrangementMateRatioRescue(bool encounteredM) { - auto candidateCount = 0; - auto cumulativeMateSupport = 0.0; - auto maxExpectedDiscordants = 0; - for (const auto &sa : suppAlignments) { - if (sa.isDistant() && sa.isEncounteredM() == encounteredM && - !sa.isSuspicious() && sa.getMateSupport() > 4) { - ++candidateCount; - if (candidateCount == 3) { - return; + for (const auto &sa : tmpBp.getSupplementsPrimary()) { + if (sa.getChrIndex() < 1002) { + suppAlignments.emplace_back(sa); } - cumulativeMateSupport += sa.getMateSupport(); - maxExpectedDiscordants = - max(maxExpectedDiscordants, sa.getExpectedDiscordants()); } + complexRearrangementMateRatioRescue(true); + complexRearrangementMateRatioRescue(false); } - if (candidateCount == 2 && - cumulativeMateSupport / maxExpectedDiscordants > 0.7) { - for (auto &sa : suppAlignments) { + + void + BreakpointReduced::complexRearrangementMateRatioRescue(bool encounteredM) { + auto candidateCount = 0; + auto cumulativeMateSupport = 0.0; + auto maxExpectedDiscordants = 0; + for (const auto &sa : suppAlignments) { if (sa.isDistant() && sa.isEncounteredM() == encounteredM && !sa.isSuspicious() && sa.getMateSupport() > 4) { - sa.setExpectedDiscordants(sa.getMateSupport()); + ++candidateCount; + if (candidateCount == 3) { + return; + } + cumulativeMateSupport += sa.getMateSupport(); + maxExpectedDiscordants = + max(maxExpectedDiscordants, sa.getExpectedDiscordants()); + } + } + if (candidateCount == 2 && + cumulativeMateSupport / maxExpectedDiscordants > 0.7) { + for (auto &sa : suppAlignments) { + if (sa.isDistant() && sa.isEncounteredM() == encounteredM && + !sa.isSuspicious() && sa.getMateSupport() > 4) { + sa.setExpectedDiscordants(sa.getMateSupport()); + } } } } -} -sophia::BreakpointReduced::BreakpointReduced( - const SuppAlignmentAnno &sa, const BreakpointReduced &emittingBp, - bool fuzzySecondary) - : hasOverhang{false}, - toRemove{false}, lineIndex{-1}, - chrIndex{sa.getChrIndex()}, pos{!fuzzySecondary ? sa.getPos() : sa.getExtendedPos()}, - normalSpans{}, - lowQualSpansSoft{}, - lowQualSpansHard{}, - unpairedBreaksSoft{}, - unpairedBreaksHard{}, - breaksShortIndel{}, - lowQualBreaksSoft{}, - lowQualBreaksHard{}, - repetitiveOverhangBreaks{}, - pairedBreaksSoft{}, - pairedBreaksHard{}, - mateSupport{}, - leftCoverage{}, - rightCoverage{}, - mrefHits{MrefMatch{-1, -1, 10000, {}}}, - germlineInfo{GermlineMatch{0.0, 0.0, {}}}, - suppAlignments{} { - addDummySa(sa, emittingBp); -} + sophia::BreakpointReduced::BreakpointReduced( + const SuppAlignmentAnno &sa, const BreakpointReduced &emittingBp, + bool fuzzySecondary) + : hasOverhang{false}, + toRemove{false}, lineIndex{-1}, + chrIndex{sa.getChrIndex()}, pos{!fuzzySecondary ? sa.getPos() : sa.getExtendedPos()}, + normalSpans{}, + lowQualSpansSoft{}, + lowQualSpansHard{}, + unpairedBreaksSoft{}, + unpairedBreaksHard{}, + breaksShortIndel{}, + lowQualBreaksSoft{}, + lowQualBreaksHard{}, + repetitiveOverhangBreaks{}, + pairedBreaksSoft{}, + pairedBreaksHard{}, + mateSupport{}, + leftCoverage{}, + rightCoverage{}, + mrefHits{MrefMatch{-1, -1, 10000, {}}}, + germlineInfo{GermlineMatch{0.0, 0.0, {}}}, + suppAlignments{} { + addDummySa(sa, emittingBp); + } -void -sophia::BreakpointReduced::addDummySa(const SuppAlignmentAnno &sa, - const BreakpointReduced &emittingBp) { - suppAlignments.emplace_back(emittingBp.getChrIndex(), emittingBp.getPos(), - sa); -} + void + sophia::BreakpointReduced::addDummySa(const SuppAlignmentAnno &sa, + const BreakpointReduced &emittingBp) { + suppAlignments.emplace_back(emittingBp.getChrIndex(), emittingBp.getPos(), + sa); + } -const SuppAlignmentAnno & -sophia::BreakpointReduced::getDummySa() { - return suppAlignments.back(); -} + const SuppAlignmentAnno & + sophia::BreakpointReduced::getDummySa() { + return suppAlignments.back(); + } -SuppAlignmentAnno * -BreakpointReduced::searchFuzzySa(const SuppAlignmentAnno &fuzzySa) { - SuppAlignmentAnno *match = nullptr; - for (auto &sa : suppAlignments) { - if (sa.saClosenessDirectional(fuzzySa, DEFAULTREADLENGTH * 0.2)) { - match = &sa; - return match; + SuppAlignmentAnno * + BreakpointReduced::searchFuzzySa(const SuppAlignmentAnno &fuzzySa) { + SuppAlignmentAnno *match = nullptr; + for (auto &sa : suppAlignments) { + if (sa.saClosenessDirectional(fuzzySa, DEFAULTREADLENGTH * 0.2)) { + match = &sa; + return match; + } } + return nullptr; } - return nullptr; -} -bool -BreakpointReduced::testOverhangBasedCandidacy() const { - if (pairedBreaksSoft > 0) { - return false; - } - if (breaksShortIndel > 0) { - return false; - } - if (unpairedBreaksSoft < 5) { - return false; - } - if (((0.0 + unpairedBreaksSoft) / normalSpans) < - CLONALITYSTRICTLOWTHRESHOLD) { - return false; - } - auto artifactTotal = - 0.0 + lowQualSpansSoft + lowQualBreaksSoft + repetitiveOverhangBreaks; - if ((artifactTotal / (unpairedBreaksSoft + artifactTotal)) > - ARTIFACTFREQHIGHTHRESHOLD) { - return false; + bool + BreakpointReduced::testOverhangBasedCandidacy() const { + if (pairedBreaksSoft > 0) { + return false; + } + if (breaksShortIndel > 0) { + return false; + } + if (unpairedBreaksSoft < 5) { + return false; + } + if (((0.0 + unpairedBreaksSoft) / normalSpans) < + CLONALITYSTRICTLOWTHRESHOLD) { + return false; + } + auto artifactTotal = + 0.0 + lowQualSpansSoft + lowQualBreaksSoft + repetitiveOverhangBreaks; + if ((artifactTotal / (unpairedBreaksSoft + artifactTotal)) > + ARTIFACTFREQHIGHTHRESHOLD) { + return false; + } + return true; } - return true; -} -string -BreakpointReduced::printOverhang(double germlineClonality, - int numHits, - const string &overhang) const { - string res{"##"}; - res.append(GlobalAppConfig::getInstance().getChrConverter().indexToChr[chrIndex]).append("\t"); - res.append(strtk::type_to_string(pos - 1)).append("\t"); - res.append(strtk::type_to_string(pos)).append("\t"); - if (germlineClonality > 0.1) { - res.append("GERMLINE("); - } else { - res.append("SOMATIC("); + string + BreakpointReduced::printOverhang(double germlineClonality, + int numHits, + const string &overhang) const { + string res{"##"}; + res.append(GlobalAppConfig::getInstance().getChrConverter().indexToChr[chrIndex]).append("\t"); + res.append(strtk::type_to_string(pos - 1)).append("\t"); + res.append(strtk::type_to_string(pos)).append("\t"); + if (germlineClonality > 0.1) { + res.append("GERMLINE("); + } else { + res.append("SOMATIC("); + } + res.append(strtk::type_to_string(numHits)) + .append("/") + .append(PIDSINMREFSTR) + .append("):"); + res.append(boost::str(doubleFormatter % germlineClonality)).append("\t"); + res.append(overhang).append("\n"); + return res; } - res.append(strtk::type_to_string(numHits)) - .append("/") - .append(PIDSINMREFSTR) - .append("):"); - res.append(boost::str(doubleFormatter % germlineClonality)).append("\t"); - res.append(overhang).append("\n"); - return res; -} -void -BreakpointReduced::removeMarkedFuzzies() { - suppAlignments.erase( - remove_if(suppAlignments.begin(), suppAlignments.end(), - [](const SuppAlignmentAnno &sa) { return sa.isToRemove(); }), - suppAlignments.end()); -} + void + BreakpointReduced::removeMarkedFuzzies() { + suppAlignments.erase( + remove_if(suppAlignments.begin(), suppAlignments.end(), + [](const SuppAlignmentAnno &sa) { return sa.isToRemove(); }), + suppAlignments.end()); + } } /* namespace sophia */ diff --git a/src/ChosenBp.cpp b/src/ChosenBp.cpp index ac519d1..563726d 100644 --- a/src/ChosenBp.cpp +++ b/src/ChosenBp.cpp @@ -27,39 +27,37 @@ namespace sophia { -using namespace std; + int ChosenBp::BPSUPPORTTHRESHOLD{}; -int ChosenBp::BPSUPPORTTHRESHOLD{}; - -void -ChosenBp::addChildNode(int indexIn) { - childrenNodes.push_back(indexIn); -} + void + ChosenBp::addChildNode(int indexIn) { + childrenNodes.push_back(indexIn); + } -void -ChosenBp::addSupplementaryAlignments( - const vector &suppAlignments) { - for (const auto &sa : suppAlignments) { - auto it = find_if(supplementaryAlignments.begin(), - supplementaryAlignments.end(), - [&](const SuppAlignment &suppAlignment) { - return suppAlignment.saCloseness(sa, 5); - }); - if (it == supplementaryAlignments.end()) { - supplementaryAlignments.push_back(sa); - } else { - if (it->isFuzzy() && !sa.isFuzzy()) { - it->removeFuzziness(sa); - } else if (it->isFuzzy() && sa.isFuzzy()) { - it->extendSuppAlignment(sa.getPos(), sa.getExtendedPos()); - } - // it->addSupportingIndices(sa.getSupportingIndices()); - if (sa.getMapq() > it->getMapq()) { - it->setMapq(sa.getMapq()); + void + ChosenBp::addSupplementaryAlignments( + const vector &suppAlignments) { + for (const auto &sa : suppAlignments) { + auto it = find_if(supplementaryAlignments.begin(), + supplementaryAlignments.end(), + [&](const SuppAlignment &suppAlignment) { + return suppAlignment.saCloseness(sa, 5); + }); + if (it == supplementaryAlignments.end()) { + supplementaryAlignments.push_back(sa); + } else { + if (it->isFuzzy() && !sa.isFuzzy()) { + it->removeFuzziness(sa); + } else if (it->isFuzzy() && sa.isFuzzy()) { + it->extendSuppAlignment(sa.getPos(), sa.getExtendedPos()); + } + // it->addSupportingIndices(sa.getSupportingIndices()); + if (sa.getMapq() > it->getMapq()) { + it->setMapq(sa.getMapq()); + } + it->incrementDistinctReads(); } - it->incrementDistinctReads(); } } -} } // namespace sophia diff --git a/src/ChrConverter.cpp b/src/ChrConverter.cpp index c20c238..7db6566 100644 --- a/src/ChrConverter.cpp +++ b/src/ChrConverter.cpp @@ -23,8 +23,6 @@ namespace sophia { - using namespace std; - ChrConverter::ChrConverter(const vector &indexToChr, const vector &indexToChrCompressedMref, const vector &chrSizesCompressedMref, diff --git a/src/DeFuzzier.cpp b/src/DeFuzzier.cpp index dff1fad..d6fb066 100644 --- a/src/DeFuzzier.cpp +++ b/src/DeFuzzier.cpp @@ -24,240 +24,238 @@ #include namespace sophia { - - using namespace std; -DeFuzzier::DeFuzzier(int maxDistanceIn, bool mrefModeIn) : - MAXDISTANCE { maxDistanceIn }, - MREFMODE { mrefModeIn } { -} + DeFuzzier::DeFuzzier(int maxDistanceIn, bool mrefModeIn) : + MAXDISTANCE { maxDistanceIn }, + MREFMODE { mrefModeIn } { + } -void DeFuzzier::deFuzzyDb(vector& bps) const { - for (auto it = bps.begin(); it != bps.end(); ++it) { - for (auto &sa : it->getSupplementsPtr()) { - if (sa->isFuzzy()) { - auto saTmp = sa; - processFuzzySa(bps, it, saTmp); - } - } - it->removeMarkedFuzzies(); - } - for (auto &bp : bps) { - bp.removeMarkedFuzzies(); - } -} + void DeFuzzier::deFuzzyDb(vector& bps) const { + for (auto it = bps.begin(); it != bps.end(); ++it) { + for (auto &sa : it->getSupplementsPtr()) { + if (sa->isFuzzy()) { + auto saTmp = sa; + processFuzzySa(bps, it, saTmp); + } + } + it->removeMarkedFuzzies(); + } + for (auto &bp : bps) { + bp.removeMarkedFuzzies(); + } + } -void DeFuzzier::processFuzzySa(vector& bps, - vector::iterator startingIt, - SuppAlignmentAnno* startingSa) const { - auto consensusSa = startingSa; - vector processedSas { startingSa }; - if (!startingSa->isEncounteredM()) { - dbSweep(bps, startingIt, 1, consensusSa, processedSas); - dbSweep(bps, startingIt, -1, consensusSa, processedSas); - } else { - dbSweep(bps, startingIt, -1, consensusSa, processedSas); - dbSweep(bps, startingIt, 1, consensusSa, processedSas); - } - selectBestSa(processedSas, consensusSa); -} + void DeFuzzier::processFuzzySa(vector& bps, + vector::iterator startingIt, + SuppAlignmentAnno* startingSa) const { + auto consensusSa = startingSa; + vector processedSas { startingSa }; + if (!startingSa->isEncounteredM()) { + dbSweep(bps, startingIt, 1, consensusSa, processedSas); + dbSweep(bps, startingIt, -1, consensusSa, processedSas); + } else { + dbSweep(bps, startingIt, -1, consensusSa, processedSas); + dbSweep(bps, startingIt, 1, consensusSa, processedSas); + } + selectBestSa(processedSas, consensusSa); + } -void DeFuzzier::dbSweep(vector& bps, - vector::iterator startingIt, - int increment, - SuppAlignmentAnno* consensusSa, - vector& processedSas) const { - auto it = startingIt; - if (it == bps.begin() || it == bps.end()) { - return; - } - advance(it, increment); - while (it != bps.begin() && it != bps.end()) { - auto res = it->searchFuzzySa(*consensusSa); - if (!res && abs(startingIt->getPos() - it->getPos()) > MAXDISTANCE) { - break; - } else { - if (res) { - processedSas.push_back(res); - if (res->isFuzzy()) { - consensusSa->extendSuppAlignment(min(res->getPos(), - consensusSa->getPos()), - max(res->getExtendedPos(), - consensusSa->getExtendedPos())); - } - } - } - advance(it, increment); - } -} + void DeFuzzier::dbSweep(vector& bps, + vector::iterator startingIt, + int increment, + SuppAlignmentAnno* consensusSa, + vector& processedSas) const { + auto it = startingIt; + if (it == bps.begin() || it == bps.end()) { + return; + } + advance(it, increment); + while (it != bps.begin() && it != bps.end()) { + auto res = it->searchFuzzySa(*consensusSa); + if (!res && abs(startingIt->getPos() - it->getPos()) > MAXDISTANCE) { + break; + } else { + if (res) { + processedSas.push_back(res); + if (res->isFuzzy()) { + consensusSa->extendSuppAlignment(min(res->getPos(), + consensusSa->getPos()), + max(res->getExtendedPos(), + consensusSa->getExtendedPos())); + } + } + } + advance(it, increment); + } + } -void DeFuzzier::selectBestSa(vector& processedSas, - SuppAlignmentAnno* consensusSa) const { - auto maxMateScore = -1; - auto maxExpectedDiscordants = -1; - auto index = 0; - vector nonFuzzyIndices { }; - for (auto &sa : processedSas) { - if (sa->getMateSupport() > maxMateScore) { - maxMateScore = sa->getMateSupport(); - } - if (sa->getExpectedDiscordants() > maxExpectedDiscordants) { - maxExpectedDiscordants = sa->getExpectedDiscordants(); - } - if (!sa->isFuzzy()) { - nonFuzzyIndices.push_back(index); - } - sa->setToRemove(true); - ++index; - } - SuppAlignmentAnno* selectedSa = nullptr; - if (!nonFuzzyIndices.empty()) { - auto bestElement = max_element(nonFuzzyIndices.begin(), nonFuzzyIndices.end(), // - [&](int a, int b) { - return processedSas[a]->getSupport()+processedSas[a]->getSecondarySupport() < processedSas[b]->getSupport()+processedSas[b]->getSecondarySupport(); - }); - selectedSa = processedSas[*bestElement]; - } else { - auto bestElement = max_element(processedSas.begin(), processedSas.end(), // - [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) { - return a->getMateSupport() < b->getMateSupport(); - }); - selectedSa = *bestElement; - selectedSa->extendSuppAlignment(consensusSa->getPos(), consensusSa->getExtendedPos()); - } - selectedSa->setToRemove(false); - selectedSa->setMateSupport(maxMateScore); - selectedSa->setExpectedDiscordants(maxExpectedDiscordants); -} + void DeFuzzier::selectBestSa(vector& processedSas, + SuppAlignmentAnno* consensusSa) const { + auto maxMateScore = -1; + auto maxExpectedDiscordants = -1; + auto index = 0; + vector nonFuzzyIndices { }; + for (auto &sa : processedSas) { + if (sa->getMateSupport() > maxMateScore) { + maxMateScore = sa->getMateSupport(); + } + if (sa->getExpectedDiscordants() > maxExpectedDiscordants) { + maxExpectedDiscordants = sa->getExpectedDiscordants(); + } + if (!sa->isFuzzy()) { + nonFuzzyIndices.push_back(index); + } + sa->setToRemove(true); + ++index; + } + SuppAlignmentAnno* selectedSa = nullptr; + if (!nonFuzzyIndices.empty()) { + auto bestElement = max_element(nonFuzzyIndices.begin(), nonFuzzyIndices.end(), // + [&](int a, int b) { + return processedSas[a]->getSupport()+processedSas[a]->getSecondarySupport() < processedSas[b]->getSupport()+processedSas[b]->getSecondarySupport(); + }); + selectedSa = processedSas[*bestElement]; + } else { + auto bestElement = max_element(processedSas.begin(), processedSas.end(), // + [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) { + return a->getMateSupport() < b->getMateSupport(); + }); + selectedSa = *bestElement; + selectedSa->extendSuppAlignment(consensusSa->getPos(), consensusSa->getExtendedPos()); + } + selectedSa->setToRemove(false); + selectedSa->setMateSupport(maxMateScore); + selectedSa->setExpectedDiscordants(maxExpectedDiscordants); + } -void DeFuzzier::deFuzzyDb(vector& bps) const { - for (auto it = bps.begin(); it != bps.end(); ++it) { - if (it->getPos() == -1) { - continue; - } - for (auto &sa : it->getSupplementsPtr()) { - if (sa->isToRemove()) { - continue; - } - if (sa->isFuzzy() || sa->isStrictFuzzy()) { - auto saTmp = sa; - processFuzzySa(bps, it, saTmp); - } - } - it->removeMarkedFuzzies(); - if (it->getValidityScore() == 0 && it->getSuppAlignments().empty()) { - it->setAsInvalid(); - } - } - for (auto &bp : bps) { - if (bp.getValidityScore() == -1 || bp.getPos() == -1) { - bp.setAsInvalid(); - } - } -} + void DeFuzzier::deFuzzyDb(vector& bps) const { + for (auto it = bps.begin(); it != bps.end(); ++it) { + if (it->getPos() == -1) { + continue; + } + for (auto &sa : it->getSupplementsPtr()) { + if (sa->isToRemove()) { + continue; + } + if (sa->isFuzzy() || sa->isStrictFuzzy()) { + auto saTmp = sa; + processFuzzySa(bps, it, saTmp); + } + } + it->removeMarkedFuzzies(); + if (it->getValidityScore() == 0 && it->getSuppAlignments().empty()) { + it->setAsInvalid(); + } + } + for (auto &bp : bps) { + if (bp.getValidityScore() == -1 || bp.getPos() == -1) { + bp.setAsInvalid(); + } + } + } -void DeFuzzier::processFuzzySa(vector& bps, - vector::iterator startingIt, - SuppAlignmentAnno* startingSa) const { - auto consensusSa = startingSa; - unordered_set fileIndices { }; - for (auto fileIndex : startingIt->getFileIndices()) { - fileIndices.insert(fileIndex); - } - vector processedSas { startingSa }; - if (!startingSa->isEncounteredM()) { - dbSweep(bps, startingIt, fileIndices, 1, consensusSa, processedSas); - dbSweep(bps, startingIt, fileIndices, -1, consensusSa, processedSas); - } else { - dbSweep(bps, startingIt, fileIndices, -1, consensusSa, processedSas); - dbSweep(bps, startingIt, fileIndices, 1, consensusSa, processedSas); - } - selectBestSa(processedSas, consensusSa, fileIndices); -} + void DeFuzzier::processFuzzySa(vector& bps, + vector::iterator startingIt, + SuppAlignmentAnno* startingSa) const { + auto consensusSa = startingSa; + unordered_set fileIndices { }; + for (auto fileIndex : startingIt->getFileIndices()) { + fileIndices.insert(fileIndex); + } + vector processedSas { startingSa }; + if (!startingSa->isEncounteredM()) { + dbSweep(bps, startingIt, fileIndices, 1, consensusSa, processedSas); + dbSweep(bps, startingIt, fileIndices, -1, consensusSa, processedSas); + } else { + dbSweep(bps, startingIt, fileIndices, -1, consensusSa, processedSas); + dbSweep(bps, startingIt, fileIndices, 1, consensusSa, processedSas); + } + selectBestSa(processedSas, consensusSa, fileIndices); + } -void DeFuzzier::dbSweep(vector& bps, - vector::iterator startingIt, - unordered_set& fileIndices, - int increment, - SuppAlignmentAnno* consensusSa, - vector& processedSas) const { - auto it = startingIt; - if (it == bps.begin() || it == bps.end()) { - return; - } - advance(it, increment); - while (it != bps.begin() && it != bps.end()) { - if (it->getPos() != -1) { - auto res = it->searchFuzzySa(*consensusSa); - if (!res && abs(startingIt->getPos() - it->getPos()) > MAXDISTANCE) { - break; - } else { - if (res) { - for (auto fileIndex : it->getFileIndices()) { - fileIndices.insert(fileIndex); - } - processedSas.push_back(res); - if (res->isFuzzy()) { - consensusSa->extendSuppAlignment(min(res->getPos(), - consensusSa->getPos()), - max(res->getExtendedPos(), - consensusSa->getExtendedPos())); - } - } - } - } - advance(it, increment); - } -} + void DeFuzzier::dbSweep(vector& bps, + vector::iterator startingIt, + unordered_set& fileIndices, + int increment, + SuppAlignmentAnno* consensusSa, + vector& processedSas) const { + auto it = startingIt; + if (it == bps.begin() || it == bps.end()) { + return; + } + advance(it, increment); + while (it != bps.begin() && it != bps.end()) { + if (it->getPos() != -1) { + auto res = it->searchFuzzySa(*consensusSa); + if (!res && abs(startingIt->getPos() - it->getPos()) > MAXDISTANCE) { + break; + } else { + if (res) { + for (auto fileIndex : it->getFileIndices()) { + fileIndices.insert(fileIndex); + } + processedSas.push_back(res); + if (res->isFuzzy()) { + consensusSa->extendSuppAlignment(min(res->getPos(), + consensusSa->getPos()), + max(res->getExtendedPos(), + consensusSa->getExtendedPos())); + } + } + } + } + advance(it, increment); + } + } -void DeFuzzier::selectBestSa(vector& processedSas, - SuppAlignmentAnno* consensusSa, - const unordered_set& fileIndices) const { - auto maxMateScore = -1; - auto maxExpectedDiscordants = -1; - auto index = 0; - vector nonFuzzyIndices { }; - for (auto &sa : processedSas) { - if (sa->getMateSupport() > maxMateScore) { - maxMateScore = sa->getMateSupport(); - } - if (sa->getExpectedDiscordants() > maxExpectedDiscordants) { - maxExpectedDiscordants = sa->getExpectedDiscordants(); - } - if (!sa->isFuzzy()) { - nonFuzzyIndices.push_back(index); - } - sa->setToRemove(true); - ++index; - } - SuppAlignmentAnno* selectedSa = nullptr; - if (!nonFuzzyIndices.empty()) { - auto bestElement = max_element(nonFuzzyIndices.begin(), nonFuzzyIndices.end(), // - [&](int a, int b) { - return processedSas[a]->getSupportingIndices().size() < processedSas[b]->getSupportingIndices().size(); - }); - selectedSa = processedSas[*bestElement]; - } else { - auto bestElement = max_element(processedSas.begin(), processedSas.end(), // - [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) { - return a->getSupportingIndices().size() < b->getSupportingIndices().size(); - }); - if ((*bestElement)->getSupport() + (*bestElement)->getSecondarySupport() == 0) { - if (consensusSa->isEncounteredM()) { - selectedSa = processedSas.back(); - } else { - selectedSa = processedSas[0]; - } - } else { - selectedSa = *bestElement; - } - selectedSa->extendSuppAlignment(consensusSa->getPos(), consensusSa->getExtendedPos()); - } - selectedSa->setToRemove(false); - selectedSa->setMateSupport(maxMateScore); - selectedSa->setExpectedDiscordants(maxExpectedDiscordants); - selectedSa->mrefSaConsensus(fileIndices); -} + void DeFuzzier::selectBestSa(vector& processedSas, + SuppAlignmentAnno* consensusSa, + const unordered_set& fileIndices) const { + auto maxMateScore = -1; + auto maxExpectedDiscordants = -1; + auto index = 0; + vector nonFuzzyIndices { }; + for (auto &sa : processedSas) { + if (sa->getMateSupport() > maxMateScore) { + maxMateScore = sa->getMateSupport(); + } + if (sa->getExpectedDiscordants() > maxExpectedDiscordants) { + maxExpectedDiscordants = sa->getExpectedDiscordants(); + } + if (!sa->isFuzzy()) { + nonFuzzyIndices.push_back(index); + } + sa->setToRemove(true); + ++index; + } + SuppAlignmentAnno* selectedSa = nullptr; + if (!nonFuzzyIndices.empty()) { + auto bestElement = max_element(nonFuzzyIndices.begin(), nonFuzzyIndices.end(), // + [&](int a, int b) { + return processedSas[a]->getSupportingIndices().size() < processedSas[b]->getSupportingIndices().size(); + }); + selectedSa = processedSas[*bestElement]; + } else { + auto bestElement = max_element(processedSas.begin(), processedSas.end(), // + [&](SuppAlignmentAnno* a, SuppAlignmentAnno* b) { + return a->getSupportingIndices().size() < b->getSupportingIndices().size(); + }); + if ((*bestElement)->getSupport() + (*bestElement)->getSecondarySupport() == 0) { + if (consensusSa->isEncounteredM()) { + selectedSa = processedSas.back(); + } else { + selectedSa = processedSas[0]; + } + } else { + selectedSa = *bestElement; + } + selectedSa->extendSuppAlignment(consensusSa->getPos(), consensusSa->getExtendedPos()); + } + selectedSa->setToRemove(false); + selectedSa->setMateSupport(maxMateScore); + selectedSa->setExpectedDiscordants(maxExpectedDiscordants); + selectedSa->mrefSaConsensus(fileIndices); + } -} +} /* namespace sophia */ diff --git a/src/GermlineMatch.cpp b/src/GermlineMatch.cpp index 9ad359f..5bbe662 100644 --- a/src/GermlineMatch.cpp +++ b/src/GermlineMatch.cpp @@ -26,17 +26,15 @@ namespace sophia { -using namespace std; - -GermlineMatch::GermlineMatch( - double clonalityIn, double conservativeClonalityIn, - const vector> &suppMatchesIn) - : clonality{clonalityIn}, conservativeClonality{conservativeClonalityIn}, - suppMatches{}, clonalities{} { - for (const auto &saPair : suppMatchesIn) { - suppMatches.push_back(saPair.first); - clonalities.push_back(saPair.second); + GermlineMatch::GermlineMatch( + double clonalityIn, double conservativeClonalityIn, + const vector> &suppMatchesIn) + : clonality{clonalityIn}, conservativeClonality{conservativeClonalityIn}, + suppMatches{}, clonalities{} { + for (const auto &saPair : suppMatchesIn) { + suppMatches.push_back(saPair.first); + clonalities.push_back(saPair.second); + } } -} } /* namespace sophia */ diff --git a/src/GlobalAppConfig.cpp b/src/GlobalAppConfig.cpp index ed19901..b31bb09 100644 --- a/src/GlobalAppConfig.cpp +++ b/src/GlobalAppConfig.cpp @@ -26,8 +26,6 @@ namespace sophia { - using namespace std; - GlobalAppConfig* GlobalAppConfig::instance_ = nullptr; std::mutex GlobalAppConfig::mutex_ = std::mutex(); @@ -59,4 +57,4 @@ namespace sophia { } } -} \ No newline at end of file +} /* namespace sophia */ \ No newline at end of file diff --git a/src/HelperFunctions.cpp b/src/HelperFunctions.cpp index f5bdd57..6184874 100644 --- a/src/HelperFunctions.cpp +++ b/src/HelperFunctions.cpp @@ -24,8 +24,6 @@ namespace sophia { - using namespace std; - istream & error_terminating_getline(istream &is, string &str) { getline(is, str); diff --git a/src/Hg37ChrConverter.cpp b/src/Hg37ChrConverter.cpp index e57dddd..d90bac8 100644 --- a/src/Hg37ChrConverter.cpp +++ b/src/Hg37ChrConverter.cpp @@ -24,11 +24,9 @@ namespace sophia { - using namespace std; - namespace hg37 { - static const vector indexToChr { + static const vector indexToChr { "0", "1", "2", "3", "4", "5", "6", "7", "8", "9", "10", "11", "12", "13", "14", @@ -231,7 +229,7 @@ namespace sophia { "995", "996", "997", "998", "hs37d5", "NC_007605", "MT", "phiX174", "INVALID"}; - static const vector indexToChrCompressedMref { + static const vector indexToChrCompressedMref { "1", "2", "3", "4", "5", "6", "7", "8", "9", "10", "11", "12", "13", "14", "15", @@ -324,7 +322,7 @@ namespace sophia { } /* namespace hg37 */ - const string Hg37ChrConverter::assembly_name = "hg37"; + const std::string Hg37ChrConverter::assembly_name = "hg37"; Hg37ChrConverter::Hg37ChrConverter() : ChrConverter(hg37::indexToChr, @@ -334,7 +332,7 @@ namespace sophia { /* This is parsing code. It takes a position in a character stream, and translates the following character(s) into index positions (see ChrConverter::indexToChr). */ - int Hg37ChrConverter::readChromosomeIndex(string::const_iterator startIt, + int Hg37ChrConverter::readChromosomeIndex(std::string::const_iterator startIt, char stopChar) const { int chrIndex {0}; if (isdigit(*startIt)) { diff --git a/src/Hg38ChrConverter.cpp b/src/Hg38ChrConverter.cpp index 2ca7ade..9ef4691 100644 --- a/src/Hg38ChrConverter.cpp +++ b/src/Hg38ChrConverter.cpp @@ -24,11 +24,9 @@ namespace sophia { - using namespace std; - namespace hg38 { - static const vector indexToChr { + static const vector indexToChr { "0", "1", "2", "3", "4", "5", "6", "7", "8", "9", "10", "11", "12", "13", "14", @@ -231,7 +229,7 @@ namespace sophia { "995", "996", "997", "998", "hs37d5", "NC_007605", "MT", "phiX174", "INVALID"}; - static const vector indexToChrCompressedMref { + static const vector indexToChrCompressedMref { "1", "2", "3", "4", "5", "6", "7", "8", "9", "10", "11", "12", "13", "14", "15", @@ -324,7 +322,7 @@ namespace sophia { } /* namespace hg38 */ - const string Hg38ChrConverter::assembly_name = "hg38"; + const std::string Hg38ChrConverter::assembly_name = "hg38"; Hg38ChrConverter::Hg38ChrConverter() : ChrConverter(hg38::indexToChr, @@ -334,7 +332,7 @@ namespace sophia { /* This is parsing code. It takes a position in a character stream, and translates the following character(s) into index positions (see ChrConverter::indexToChr). */ - int Hg38ChrConverter::readChromosomeIndex(string::const_iterator startIt, + int Hg38ChrConverter::readChromosomeIndex(std::string::const_iterator startIt, char stopChar) const { int chrIndex {0}; if (isdigit(*startIt)) { diff --git a/src/MasterRefProcessor.cpp b/src/MasterRefProcessor.cpp index b4ac069..ed4b1f7 100644 --- a/src/MasterRefProcessor.cpp +++ b/src/MasterRefProcessor.cpp @@ -36,144 +36,142 @@ namespace sophia { -using namespace std; + MasterRefProcessor::MasterRefProcessor(const vector &filesIn, + const string &outputRootName, + const string &version, + const int defaultReadLengthIn) + : NUMPIDS{static_cast(filesIn.size())}, + DEFAULTREADLENGTH{defaultReadLengthIn}, + mrefDb{} { -MasterRefProcessor::MasterRefProcessor(const vector &filesIn, - const string &outputRootName, - const string &version, - const int defaultReadLengthIn) - : NUMPIDS{static_cast(filesIn.size())}, - DEFAULTREADLENGTH{defaultReadLengthIn}, - mrefDb{} { - - const vector &chrSizes = - GlobalAppConfig::getInstance().getChrConverter().chrSizesCompressedMref; - for (std::vector::size_type i = 0; i < chrSizes.size(); ++i) { - // mrefDbPtrs.emplace_back(chrSizes[i] + 1, nullptr); - mrefDb.emplace_back(chrSizes[i] + 1, MrefEntry{}); - } - vector header{"#chr", "start", "end"}; - for (const auto &gzFile : filesIn) { - int posOnVersion = version.size() - 1; - bool counting{false}; - string realPidName; - for (auto rit = gzFile.crbegin(); rit != gzFile.crend(); ++rit) { - if (!counting) { - if (*rit != version[posOnVersion]) { - posOnVersion = version.size() - 1; - } else { - --posOnVersion; - if (posOnVersion == -1) { - ++rit; - counting = true; + const vector &chrSizes = + GlobalAppConfig::getInstance().getChrConverter().chrSizesCompressedMref; + for (std::vector::size_type i = 0; i < chrSizes.size(); ++i) { + // mrefDbPtrs.emplace_back(chrSizes[i] + 1, nullptr); + mrefDb.emplace_back(chrSizes[i] + 1, MrefEntry{}); + } + vector header{"#chr", "start", "end"}; + for (const auto &gzFile : filesIn) { + int posOnVersion = version.size() - 1; + bool counting{false}; + string realPidName; + for (auto rit = gzFile.crbegin(); rit != gzFile.crend(); ++rit) { + if (!counting) { + if (*rit != version[posOnVersion]) { + posOnVersion = version.size() - 1; + } else { + --posOnVersion; + if (posOnVersion == -1) { + ++rit; + counting = true; + } } - } - } else { - if (*rit == '/') { - break; } else { - realPidName.push_back(*rit); + if (*rit == '/') { + break; + } else { + realPidName.push_back(*rit); + } } } + reverse(realPidName.begin(), realPidName.end()); + header.push_back(realPidName); } - reverse(realPidName.begin(), realPidName.end()); - header.push_back(realPidName); - } - mergedBpsOutput = make_unique( - outputRootName + "_" + strtk::type_to_string(NUMPIDS) + - "_mergedBpCounts.bed"); - short fileIndex{0}; - for (const auto &gzFile : filesIn) { - chrono::time_point start = - chrono::steady_clock::now(); - auto newBreakpoints = processFile(gzFile, fileIndex); - chrono::time_point end = - chrono::steady_clock::now(); - chrono::seconds diff = - chrono::duration_cast(end - start); - ++fileIndex; - cerr << gzFile << "\t" << diff.count() << "\t" << newBreakpoints << "\t" - << fileIndex << "\t" << 100 * (fileIndex + 0.0) / NUMPIDS << "%\n"; - } - auto defuzzier = DeFuzzier{DEFAULTREADLENGTH * 3, true}; - auto i = 84; - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - while (!mrefDb.empty()) { - mrefDb.back().erase( - remove_if(mrefDb.back().begin(), mrefDb.back().end(), - [](const MrefEntry &bp) { return bp.getPos() == -1; }), - mrefDb.back().end()); - defuzzier.deFuzzyDb(mrefDb.back()); - mrefDb.back().erase( - remove_if(mrefDb.back().begin(), mrefDb.back().end(), - [](const MrefEntry &bp) { return bp.getPos() == -1; }), - mrefDb.back().end()); - auto chromosome = chrConverter.indexToChrCompressedMref[i]; - --i; - for (auto &bp : mrefDb.back()) { - if (bp.getPos() != -1 && bp.getValidityScore() != -1) { - // cout << - //bp.printArtifactRatios(chromosome); - *mergedBpsOutput << bp.printBpInfo(chromosome); + mergedBpsOutput = make_unique( + outputRootName + "_" + strtk::type_to_string(NUMPIDS) + + "_mergedBpCounts.bed"); + short fileIndex{0}; + for (const auto &gzFile : filesIn) { + chrono::time_point start = + chrono::steady_clock::now(); + auto newBreakpoints = processFile(gzFile, fileIndex); + chrono::time_point end = + chrono::steady_clock::now(); + chrono::seconds diff = + chrono::duration_cast(end - start); + ++fileIndex; + cerr << gzFile << "\t" << diff.count() << "\t" << newBreakpoints << "\t" + << fileIndex << "\t" << 100 * (fileIndex + 0.0) / NUMPIDS << "%\n"; + } + auto defuzzier = DeFuzzier{DEFAULTREADLENGTH * 3, true}; + auto i = 84; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + while (!mrefDb.empty()) { + mrefDb.back().erase( + remove_if(mrefDb.back().begin(), mrefDb.back().end(), + [](const MrefEntry &bp) { return bp.getPos() == -1; }), + mrefDb.back().end()); + defuzzier.deFuzzyDb(mrefDb.back()); + mrefDb.back().erase( + remove_if(mrefDb.back().begin(), mrefDb.back().end(), + [](const MrefEntry &bp) { return bp.getPos() == -1; }), + mrefDb.back().end()); + auto chromosome = chrConverter.indexToChrCompressedMref[i]; + --i; + for (auto &bp : mrefDb.back()) { + if (bp.getPos() != -1 && bp.getValidityScore() != -1) { + // cout << + //bp.printArtifactRatios(chromosome); + *mergedBpsOutput << bp.printBpInfo(chromosome); + } } + mrefDb.pop_back(); } - mrefDb.pop_back(); } -} -unsigned long long -MasterRefProcessor::processFile(const string &gzPath, short fileIndex) { - unsigned long long newBreakpoints{0}; - ifstream refHandle(gzPath, ios_base::in | ios_base::binary); - boost::iostreams::filtering_istream gzStream{}; - gzStream.push(boost::iostreams::gzip_decompressor()); - gzStream.push(refHandle); - string sophiaLine{}; - vector> fileBps{85, vector{}}; - auto lineIndex = 0; - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - while (error_terminating_getline(gzStream, sophiaLine)) { - if (sophiaLine[0] != '#') { - auto chrIndex = - chrConverter.indexConverter[chrConverter.readChromosomeIndex( - sophiaLine.cbegin(), '\t')]; - if (chrIndex < 0) { - continue; + unsigned long long + MasterRefProcessor::processFile(const string &gzPath, short fileIndex) { + unsigned long long newBreakpoints{0}; + ifstream refHandle(gzPath, ios_base::in | ios_base::binary); + boost::iostreams::filtering_istream gzStream{}; + gzStream.push(boost::iostreams::gzip_decompressor()); + gzStream.push(refHandle); + string sophiaLine{}; + vector> fileBps{85, vector{}}; + auto lineIndex = 0; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + while (error_terminating_getline(gzStream, sophiaLine)) { + if (sophiaLine[0] != '#') { + auto chrIndex = + chrConverter.indexConverter[chrConverter.readChromosomeIndex( + sophiaLine.cbegin(), '\t')]; + if (chrIndex < 0) { + continue; + } + Breakpoint tmpBp{sophiaLine, true}; + fileBps[chrIndex].emplace_back( + tmpBp, lineIndex++, + (sophiaLine.back() != '.' && sophiaLine.back() != '#')); } - Breakpoint tmpBp{sophiaLine, true}; - fileBps[chrIndex].emplace_back( - tmpBp, lineIndex++, - (sophiaLine.back() != '.' && sophiaLine.back() != '#')); } - } - auto chrIndex = 0; - for (auto &chromosome : fileBps) { - DeFuzzier deFuzzierControl{DEFAULTREADLENGTH * 6, false}; - deFuzzierControl.deFuzzyDb(chromosome); - for (auto &bp : chromosome) { - if (processBp(bp, chrIndex, fileIndex)) { - ++newBreakpoints; + auto chrIndex = 0; + for (auto &chromosome : fileBps) { + DeFuzzier deFuzzierControl{DEFAULTREADLENGTH * 6, false}; + deFuzzierControl.deFuzzyDb(chromosome); + for (auto &bp : chromosome) { + if (processBp(bp, chrIndex, fileIndex)) { + ++newBreakpoints; + } } + ++chrIndex; } - ++chrIndex; + return newBreakpoints; } - return newBreakpoints; -} -bool -MasterRefProcessor::processBp(BreakpointReduced &bp, int chrIndex, - short fileIndex) { - MrefEntry tmpMrefEntry{}; - tmpMrefEntry.addEntry(bp, fileIndex); - auto validitiyInit = - mrefDb[chrIndex][tmpMrefEntry.getPos()].getValidityScore(); - mrefDb[chrIndex][tmpMrefEntry.getPos()].mergeMrefEntries(tmpMrefEntry); - auto validitiyFinal = - mrefDb[chrIndex][tmpMrefEntry.getPos()].getValidityScore(); - if (validitiyFinal > validitiyInit) { - return true; + bool + MasterRefProcessor::processBp(BreakpointReduced &bp, int chrIndex, + short fileIndex) { + MrefEntry tmpMrefEntry{}; + tmpMrefEntry.addEntry(bp, fileIndex); + auto validitiyInit = + mrefDb[chrIndex][tmpMrefEntry.getPos()].getValidityScore(); + mrefDb[chrIndex][tmpMrefEntry.getPos()].mergeMrefEntries(tmpMrefEntry); + auto validitiyFinal = + mrefDb[chrIndex][tmpMrefEntry.getPos()].getValidityScore(); + if (validitiyFinal > validitiyInit) { + return true; + } + return false; } - return false; -} } // namespace sophia diff --git a/src/MrefEntry.cpp b/src/MrefEntry.cpp index dc4823f..00403b6 100644 --- a/src/MrefEntry.cpp +++ b/src/MrefEntry.cpp @@ -31,235 +31,233 @@ namespace sophia { -using namespace std; + boost::format MrefEntry::doubleFormatter { "%.5f" }; + int MrefEntry::NUMPIDS { }; + int MrefEntry::DEFAULTREADLENGTH { }; -boost::format MrefEntry::doubleFormatter { "%.5f" }; -int MrefEntry::NUMPIDS { }; -int MrefEntry::DEFAULTREADLENGTH { }; + MrefEntry::MrefEntry() : + validity { -1 }, + pos { -1 }, + fileIndices { }, + fileIndicesWithArtifactRatios { }, + artifactRatios { }, + suppAlignments { } { -MrefEntry::MrefEntry() : - validity { -1 }, - pos { -1 }, - fileIndices { }, - fileIndicesWithArtifactRatios { }, - artifactRatios { }, - suppAlignments { } { + } -} + void MrefEntry::addEntry(BreakpointReduced& tmpBreakpoint, int fileIndex) { + pos = tmpBreakpoint.getPos(); + auto artifactBreakTotal = + tmpBreakpoint.getLowQualBreaksSoft() + + tmpBreakpoint.getLowQualBreaksHard() + + tmpBreakpoint.getRepetitiveOverhangBreaks(); + auto eventTotal = + tmpBreakpoint.getPairedBreaksSoft() + + tmpBreakpoint.getPairedBreaksHard() + + tmpBreakpoint.getUnpairedBreaksSoft() + + tmpBreakpoint.getUnpairedBreaksHard() + + tmpBreakpoint.getBreaksShortIndel(); + auto breakTotal = eventTotal + artifactBreakTotal; + if (breakTotal < 200) { + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + for (auto saPtr : tmpBreakpoint.getSupplementsPtr()) { + if (saPtr->isSuspicious() + || saPtr->isToRemove() + || (saPtr->getChrIndex() != 1001 + && chrConverter.indexConverter[saPtr->getChrIndex()] < 0)) { + continue; + } + auto qualCheck = false; + auto splitTotal = saPtr->getSupport() + saPtr->getSecondarySupport(); + if (saPtr->isDistant()) { + auto clonalityCondition = + (((0.0 + saPtr->getMateSupport()) / saPtr->getExpectedDiscordants()) >= 0.5); + if (!clonalityCondition) { + continue; + } + qualCheck = (splitTotal > 4 && saPtr->getMateSupport() > 2); + if (!qualCheck) { + qualCheck = (saPtr->getMateSupport() > 4); + } + } else { + qualCheck = (splitTotal > 4) + || (splitTotal > 2 && saPtr->getSupport() > 0 + && saPtr->getSecondarySupport() > 0); + } + if (qualCheck) { + if (!saMatcher(saPtr)) { + auto saTmp = *saPtr; + saTmp.mrefSaTransform(fileIndex); + suppAlignments.push_back(saTmp); + } + } + } + } + auto covValidity = (tmpBreakpoint.getBreaksShortIndel() > 2 || breakTotal > 9); + if (!covValidity) { + if (breakTotal > 4) { + auto clonality = ((breakTotal + 0.0) / (tmpBreakpoint.getNormalSpans() + breakTotal)); + if (clonality > 0.3) { + covValidity = true; + } else if (clonality > 0.1) { + if (tmpBreakpoint.hasOverhang) { + covValidity = true; + } + } + } + } + if (eventTotal + artifactBreakTotal > 0) { + if (covValidity) { + auto eventTotalStrict = + tmpBreakpoint.getPairedBreaksSoft() + + tmpBreakpoint.getUnpairedBreaksSoft() + + tmpBreakpoint.getPairedBreaksHard(); + auto artifactTotalRelaxed = + tmpBreakpoint.getLowQualBreaksSoft() + + tmpBreakpoint.getLowQualSpansSoft() + + tmpBreakpoint.getRepetitiveOverhangBreaks(); + if ((eventTotalStrict + artifactTotalRelaxed) > 0) { + artifactRatios.push_back( + (0.0 + artifactTotalRelaxed) / (eventTotalStrict + artifactTotalRelaxed)); + fileIndicesWithArtifactRatios.push_back(fileIndex); + } + } + } + if (covValidity) { + fileIndices.push_back(fileIndex); + validity = 1; + } else if (!suppAlignments.empty()) { + fileIndices.push_back(fileIndex); + validity = 0; + } + } -void MrefEntry::addEntry(BreakpointReduced& tmpBreakpoint, int fileIndex) { - pos = tmpBreakpoint.getPos(); - auto artifactBreakTotal = - tmpBreakpoint.getLowQualBreaksSoft() - + tmpBreakpoint.getLowQualBreaksHard() - + tmpBreakpoint.getRepetitiveOverhangBreaks(); - auto eventTotal = - tmpBreakpoint.getPairedBreaksSoft() - + tmpBreakpoint.getPairedBreaksHard() - + tmpBreakpoint.getUnpairedBreaksSoft() - + tmpBreakpoint.getUnpairedBreaksHard() - + tmpBreakpoint.getBreaksShortIndel(); - auto breakTotal = eventTotal + artifactBreakTotal; - if (breakTotal < 200) { - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - for (auto saPtr : tmpBreakpoint.getSupplementsPtr()) { - if (saPtr->isSuspicious() - || saPtr->isToRemove() - || (saPtr->getChrIndex() != 1001 - && chrConverter.indexConverter[saPtr->getChrIndex()] < 0)) { - continue; - } - auto qualCheck = false; - auto splitTotal = saPtr->getSupport() + saPtr->getSecondarySupport(); - if (saPtr->isDistant()) { - auto clonalityCondition = - (((0.0 + saPtr->getMateSupport()) / saPtr->getExpectedDiscordants()) >= 0.5); - if (!clonalityCondition) { - continue; - } - qualCheck = (splitTotal > 4 && saPtr->getMateSupport() > 2); - if (!qualCheck) { - qualCheck = (saPtr->getMateSupport() > 4); - } - } else { - qualCheck = (splitTotal > 4) - || (splitTotal > 2 && saPtr->getSupport() > 0 - && saPtr->getSecondarySupport() > 0); - } - if (qualCheck) { - if (!saMatcher(saPtr)) { - auto saTmp = *saPtr; - saTmp.mrefSaTransform(fileIndex); - suppAlignments.push_back(saTmp); - } - } - } - } - auto covValidity = (tmpBreakpoint.getBreaksShortIndel() > 2 || breakTotal > 9); - if (!covValidity) { - if (breakTotal > 4) { - auto clonality = ((breakTotal + 0.0) / (tmpBreakpoint.getNormalSpans() + breakTotal)); - if (clonality > 0.3) { - covValidity = true; - } else if (clonality > 0.1) { - if (tmpBreakpoint.hasOverhang) { - covValidity = true; - } - } - } - } - if (eventTotal + artifactBreakTotal > 0) { - if (covValidity) { - auto eventTotalStrict = - tmpBreakpoint.getPairedBreaksSoft() - + tmpBreakpoint.getUnpairedBreaksSoft() - + tmpBreakpoint.getPairedBreaksHard(); - auto artifactTotalRelaxed = - tmpBreakpoint.getLowQualBreaksSoft() - + tmpBreakpoint.getLowQualSpansSoft() - + tmpBreakpoint.getRepetitiveOverhangBreaks(); - if ((eventTotalStrict + artifactTotalRelaxed) > 0) { - artifactRatios.push_back( - (0.0 + artifactTotalRelaxed) / (eventTotalStrict + artifactTotalRelaxed)); - fileIndicesWithArtifactRatios.push_back(fileIndex); - } - } - } - if (covValidity) { - fileIndices.push_back(fileIndex); - validity = 1; - } else if (!suppAlignments.empty()) { - fileIndices.push_back(fileIndex); - validity = 0; - } -} + void MrefEntry::mergeMrefEntries(MrefEntry& entry2) { + pos = entry2.getPos(); + for (auto artifactRatio : entry2.getArtifactRatios()) { + artifactRatios.push_back(artifactRatio); + } + for (auto fileIndex : entry2.getFileIndicesWithArtifactRatios()) { + fileIndicesWithArtifactRatios.push_back(fileIndex); + } + for (auto fileIndex : entry2.getFileIndices()) { + fileIndices.push_back(fileIndex); + } + for (auto saPtr : entry2.getSupplementsPtr()) { + if (!saMatcher(saPtr)) { + suppAlignments.push_back(*saPtr); + } + } + validity = max(validity, entry2.getValidityScore()); + } -void MrefEntry::mergeMrefEntries(MrefEntry& entry2) { - pos = entry2.getPos(); - for (auto artifactRatio : entry2.getArtifactRatios()) { - artifactRatios.push_back(artifactRatio); - } - for (auto fileIndex : entry2.getFileIndicesWithArtifactRatios()) { - fileIndicesWithArtifactRatios.push_back(fileIndex); - } - for (auto fileIndex : entry2.getFileIndices()) { - fileIndices.push_back(fileIndex); - } - for (auto saPtr : entry2.getSupplementsPtr()) { - if (!saMatcher(saPtr)) { - suppAlignments.push_back(*saPtr); - } - } - validity = max(validity, entry2.getValidityScore()); -} + string MrefEntry::printBpInfo(const string& chromosome) { + finalizeFileIndices(); + vector outputFields { }; + outputFields.emplace_back(chromosome); + outputFields.emplace_back(strtk::type_to_string(pos)); + outputFields.emplace_back(strtk::type_to_string(pos + 1)); + outputFields.emplace_back(strtk::type_to_string(fileIndices.size())); + outputFields.emplace_back(strtk::type_to_string(fileIndicesWithArtifactRatios.size())); + outputFields.emplace_back(boost::str(doubleFormatter % ((fileIndices.size() + 0.0) / NUMPIDS))); + outputFields.emplace_back(boost::str(doubleFormatter % ((fileIndicesWithArtifactRatios.size() + 0.0) / NUMPIDS))); + if (!artifactRatios.empty()) { + outputFields.emplace_back(boost::str(doubleFormatter % (accumulate(artifactRatios.cbegin(), + artifactRatios.cend(), 0.0) / artifactRatios.size()))); + } else { + outputFields.emplace_back("NA"); + } + if (suppAlignments.empty()) { + outputFields.emplace_back("."); + } else { + vector saFields { }; + saFields.reserve(suppAlignments.size()); + for (auto &sa : suppAlignments) { + sa.finalizeSupportingIndices(); + if (suppAlignments.size() < 11 || sa.getSupport() >= 0.2 * fileIndices.size()) { + saFields.emplace_back(sa.print()); + } + } + if (saFields.empty()) { + outputFields.emplace_back("."); + } else { + outputFields.emplace_back(boost::join(saFields, ";")); + } + } + vector fileIndicesStr { }; + transform(fileIndices.begin(), + fileIndices.end(), + back_inserter(fileIndicesStr), + [](int fileIndex) {return strtk::type_to_string(fileIndex);}); + outputFields.emplace_back(boost::join(fileIndicesStr, ",")); + return boost::join(outputFields, "\t").append("\n"); + } -string MrefEntry::printBpInfo(const string& chromosome) { - finalizeFileIndices(); - vector outputFields { }; - outputFields.emplace_back(chromosome); - outputFields.emplace_back(strtk::type_to_string(pos)); - outputFields.emplace_back(strtk::type_to_string(pos + 1)); - outputFields.emplace_back(strtk::type_to_string(fileIndices.size())); - outputFields.emplace_back(strtk::type_to_string(fileIndicesWithArtifactRatios.size())); - outputFields.emplace_back(boost::str(doubleFormatter % ((fileIndices.size() + 0.0) / NUMPIDS))); - outputFields.emplace_back(boost::str(doubleFormatter % ((fileIndicesWithArtifactRatios.size() + 0.0) / NUMPIDS))); - if (!artifactRatios.empty()) { - outputFields.emplace_back(boost::str(doubleFormatter % (accumulate(artifactRatios.cbegin(), - artifactRatios.cend(), 0.0) / artifactRatios.size()))); - } else { - outputFields.emplace_back("NA"); - } - if (suppAlignments.empty()) { - outputFields.emplace_back("."); - } else { - vector saFields { }; - saFields.reserve(suppAlignments.size()); - for (auto &sa : suppAlignments) { - sa.finalizeSupportingIndices(); - if (suppAlignments.size() < 11 || sa.getSupport() >= 0.2 * fileIndices.size()) { - saFields.emplace_back(sa.print()); - } - } - if (saFields.empty()) { - outputFields.emplace_back("."); - } else { - outputFields.emplace_back(boost::join(saFields, ";")); - } - } - vector fileIndicesStr { }; - transform(fileIndices.begin(), - fileIndices.end(), - back_inserter(fileIndicesStr), - [](int fileIndex) {return strtk::type_to_string(fileIndex);}); - outputFields.emplace_back(boost::join(fileIndicesStr, ",")); - return boost::join(outputFields, "\t").append("\n"); -} + string MrefEntry::printArtifactRatios(const string& chromosome) { + vector outputFields { }; + outputFields.reserve(NUMPIDS + 3); + outputFields.emplace_back(chromosome); + outputFields.emplace_back(strtk::type_to_string(pos)); + outputFields.emplace_back(strtk::type_to_string(pos + 1)); + vector artifactRatiosOutput(NUMPIDS, "."); + for (auto i = 0; i < static_cast(fileIndicesWithArtifactRatios.size()); ++i) { + artifactRatiosOutput[fileIndicesWithArtifactRatios[i]] = + boost::str(doubleFormatter % artifactRatios[i]); + } + for (const auto &artifactRatio : artifactRatiosOutput) { + outputFields.push_back(artifactRatio); + } + return boost::join(outputFields, "\t").append("\n"); + } -string MrefEntry::printArtifactRatios(const string& chromosome) { - vector outputFields { }; - outputFields.reserve(NUMPIDS + 3); - outputFields.emplace_back(chromosome); - outputFields.emplace_back(strtk::type_to_string(pos)); - outputFields.emplace_back(strtk::type_to_string(pos + 1)); - vector artifactRatiosOutput(NUMPIDS, "."); - for (auto i = 0; i < static_cast(fileIndicesWithArtifactRatios.size()); ++i) { - artifactRatiosOutput[fileIndicesWithArtifactRatios[i]] = - boost::str(doubleFormatter % artifactRatios[i]); - } - for (const auto &artifactRatio : artifactRatiosOutput) { - outputFields.push_back(artifactRatio); - } - return boost::join(outputFields, "\t").append("\n"); -} + SuppAlignmentAnno* MrefEntry::searchFuzzySa(const SuppAlignmentAnno& fuzzySa) { + SuppAlignmentAnno* match = nullptr; + for (auto &sa : suppAlignments) { + if (sa.isToRemove()) { + continue; + } + if (sa.saClosenessDirectional(fuzzySa, 1)) { + match = &sa; + return match; + } + } + return nullptr; + } -SuppAlignmentAnno* MrefEntry::searchFuzzySa(const SuppAlignmentAnno& fuzzySa) { - SuppAlignmentAnno* match = nullptr; - for (auto &sa : suppAlignments) { - if (sa.isToRemove()) { - continue; - } - if (sa.saClosenessDirectional(fuzzySa, 1)) { - match = &sa; - return match; - } - } - return nullptr; -} + bool MrefEntry::saMatcher(SuppAlignmentAnno* saPtr) { + if (saPtr->isToRemove() + || saPtr->isSuspicious() + || (saPtr->getExpectedDiscordants() > 0 + && !(saPtr->getMateSupport() / (saPtr->getExpectedDiscordants() + 0.0) > 0.1))) { + return true; + } + for (auto &sa : suppAlignments) { + if (sa.saCloseness(*saPtr, 100)) { + if (sa.isFuzzy()) { + if (saPtr->isFuzzy()) { + sa.extendSuppAlignment(saPtr->getPos(), saPtr->getExtendedPos()); + } else { + sa.removeFuzziness(*saPtr); + } + } + sa.addSupportingIndices(saPtr->getSupportingIndices()); + sa.mergeMrefSa(*saPtr); + saPtr->setToRemove(true); + return true; + } + } + return false; + } -bool MrefEntry::saMatcher(SuppAlignmentAnno* saPtr) { - if (saPtr->isToRemove() - || saPtr->isSuspicious() - || (saPtr->getExpectedDiscordants() > 0 - && !(saPtr->getMateSupport() / (saPtr->getExpectedDiscordants() + 0.0) > 0.1))) { - return true; - } - for (auto &sa : suppAlignments) { - if (sa.saCloseness(*saPtr, 100)) { - if (sa.isFuzzy()) { - if (saPtr->isFuzzy()) { - sa.extendSuppAlignment(saPtr->getPos(), saPtr->getExtendedPos()); - } else { - sa.removeFuzziness(*saPtr); - } - } - sa.addSupportingIndices(saPtr->getSupportingIndices()); - sa.mergeMrefSa(*saPtr); - saPtr->setToRemove(true); - return true; - } - } - return false; -} - -void MrefEntry::finalizeFileIndices() { - for (const auto &sa : suppAlignments) { - auto tmpIndices = sa.getSupportingIndices(); - copy(tmpIndices.begin(), tmpIndices.end(), inserter(fileIndices, fileIndices.end())); - } - sort(fileIndices.begin(), fileIndices.end()); - auto last = unique(fileIndices.begin(), fileIndices.end()); - fileIndices.erase(last, fileIndices.end()); -} + void MrefEntry::finalizeFileIndices() { + for (const auto &sa : suppAlignments) { + auto tmpIndices = sa.getSupportingIndices(); + copy(tmpIndices.begin(), tmpIndices.end(), inserter(fileIndices, fileIndices.end())); + } + sort(fileIndices.begin(), fileIndices.end()); + auto last = unique(fileIndices.begin(), fileIndices.end()); + fileIndices.erase(last, fileIndices.end()); + } } /* namespace sophia */ diff --git a/src/MrefEntryAnno.cpp b/src/MrefEntryAnno.cpp index c1afb19..0e78e4f 100644 --- a/src/MrefEntryAnno.cpp +++ b/src/MrefEntryAnno.cpp @@ -27,72 +27,71 @@ #include namespace sophia { - using namespace std; -boost::format MrefEntryAnno::doubleFormatter { "%.5f" }; -int MrefEntryAnno::DEFAULTREADLENGTH { }; -int MrefEntryAnno::PIDSINMREF { }; + boost::format MrefEntryAnno::doubleFormatter { "%.5f" }; + int MrefEntryAnno::DEFAULTREADLENGTH { }; + int MrefEntryAnno::PIDSINMREF { }; -MrefEntryAnno::MrefEntryAnno(const string& mrefEntryIn) : - pos { 0 }, - numHits { 0 }, - suppAlignments { } { - auto index = 0; - vector bpChunkPositions { }; - bpChunkPositions.reserve(7); - auto cit = mrefEntryIn.cbegin(); - if (mrefEntryIn.back() != '.') { - while (bpChunkPositions.size() < 8) { - if (*cit == '\t') { - bpChunkPositions.push_back(index); - } - ++index; - ++cit; - } - string saStr { }; - for (auto i = bpChunkPositions[7] + 1; i < static_cast(mrefEntryIn.length()); ++i) { - if (mrefEntryIn[i] == ';') { - suppAlignments.emplace_back(saStr); - saStr.clear(); - } else { - saStr.push_back(mrefEntryIn[i]); - } - } - suppAlignments.emplace_back(saStr); - } else { - while (bpChunkPositions.size() < 5) { - if (*cit == '\t') { - bpChunkPositions.push_back(index); - } - ++index; - ++cit; - } - } + MrefEntryAnno::MrefEntryAnno(const string& mrefEntryIn) : + pos { 0 }, + numHits { 0 }, + suppAlignments { } { + auto index = 0; + vector bpChunkPositions { }; + bpChunkPositions.reserve(7); + auto cit = mrefEntryIn.cbegin(); + if (mrefEntryIn.back() != '.') { + while (bpChunkPositions.size() < 8) { + if (*cit == '\t') { + bpChunkPositions.push_back(index); + } + ++index; + ++cit; + } + string saStr { }; + for (auto i = bpChunkPositions[7] + 1; i < static_cast(mrefEntryIn.length()); ++i) { + if (mrefEntryIn[i] == ';') { + suppAlignments.emplace_back(saStr); + saStr.clear(); + } else { + saStr.push_back(mrefEntryIn[i]); + } + } + suppAlignments.emplace_back(saStr); + } else { + while (bpChunkPositions.size() < 5) { + if (*cit == '\t') { + bpChunkPositions.push_back(index); + } + ++index; + ++cit; + } + } - for (auto i = bpChunkPositions[0] + 1; i < bpChunkPositions[1]; ++i) { - pos = pos * 10 + (mrefEntryIn[i] - '0'); - } - for (auto i = bpChunkPositions[2] + 1; i < bpChunkPositions[3]; ++i) { - numHits = numHits * 10 + (mrefEntryIn[i] - '0'); - } - if (mrefEntryIn[0] == 'Y') { - numHits = min(PIDSINMREF, 2 * numHits); - } - for (auto &sa : suppAlignments) { - sa.setSecondarySupport(numHits); - } + for (auto i = bpChunkPositions[0] + 1; i < bpChunkPositions[1]; ++i) { + pos = pos * 10 + (mrefEntryIn[i] - '0'); + } + for (auto i = bpChunkPositions[2] + 1; i < bpChunkPositions[3]; ++i) { + numHits = numHits * 10 + (mrefEntryIn[i] - '0'); + } + if (mrefEntryIn[0] == 'Y') { + numHits = min(PIDSINMREF, 2 * numHits); + } + for (auto &sa : suppAlignments) { + sa.setSecondarySupport(numHits); + } -} + } -//SuppAlignmentAnno* MrefEntryAnno::searchFuzzySa(const SuppAlignmentAnno& fuzzySa) { -// SuppAlignmentAnno* match = nullptr; -// for (auto &sa : suppAlignments) { -// if (sa.saCloseness(fuzzySa, 1)) { -// match = &sa; -// return match; -// } -// } -// return nullptr; -//} + //SuppAlignmentAnno* MrefEntryAnno::searchFuzzySa(const SuppAlignmentAnno& fuzzySa) { + // SuppAlignmentAnno* match = nullptr; + // for (auto &sa : suppAlignments) { + // if (sa.saCloseness(fuzzySa, 1)) { + // match = &sa; + // return match; + // } + // } + // return nullptr; + //} } /* namespace sophia */ diff --git a/src/MrefMatch.cpp b/src/MrefMatch.cpp index ddac1e4..5dfe766 100644 --- a/src/MrefMatch.cpp +++ b/src/MrefMatch.cpp @@ -27,11 +27,11 @@ namespace sophia { -MrefMatch::MrefMatch(short numHitsIn, short numConsevativeHitsIn, - int offsetDistanceIn, - const std::vector &suppMatchesIn) - : numHits{numHitsIn}, numConsevativeHits{numConsevativeHitsIn}, - offsetDistance{offsetDistanceIn}, suppMatches{suppMatchesIn} {} + MrefMatch::MrefMatch(short numHitsIn, short numConsevativeHitsIn, + int offsetDistanceIn, + const std::vector &suppMatchesIn) + : numHits{numHitsIn}, numConsevativeHits{numConsevativeHitsIn}, + offsetDistance{offsetDistanceIn}, suppMatches{suppMatchesIn} {} } // namespace sophia /* namespace sophia */ diff --git a/src/SamSegmentMapper.cpp b/src/SamSegmentMapper.cpp index 38be542..fc15caa 100644 --- a/src/SamSegmentMapper.cpp +++ b/src/SamSegmentMapper.cpp @@ -30,324 +30,287 @@ namespace sophia { -using namespace std; + SamSegmentMapper::SamSegmentMapper(int defaultReadLengthIn) + : STARTTIME{time(nullptr)}, + PROPERPARIRCOMPENSATIONMODE{Breakpoint::PROPERPAIRCOMPENSATIONMODE}, + DISCORDANTLEFTRANGE{static_cast(round(defaultReadLengthIn * 3))}, + DISCORDANTRIGHTRANGE{static_cast(round(defaultReadLengthIn * 2.51))}, + printedBps{0u}, + chrIndexCurrent{0}, + minPos{-1}, + maxPos{-1}, + breakpointsCurrent{}, + discordantAlignmentsPool{}, + discordantAlignmentCandidatesPool{}, + discordantLowQualAlignmentsPool{} {} -SamSegmentMapper::SamSegmentMapper(int defaultReadLengthIn) - : STARTTIME{time(nullptr)}, - PROPERPARIRCOMPENSATIONMODE{Breakpoint::PROPERPAIRCOMPENSATIONMODE}, - DISCORDANTLEFTRANGE{static_cast(round(defaultReadLengthIn * 3))}, - DISCORDANTRIGHTRANGE{static_cast(round(defaultReadLengthIn * 2.51))}, - printedBps{0u}, - chrIndexCurrent{0}, - minPos{-1}, - maxPos{-1}, - breakpointsCurrent{}, - discordantAlignmentsPool{}, - discordantAlignmentCandidatesPool{}, - discordantLowQualAlignmentsPool{} {} - -void -SamSegmentMapper::parseSamStream() { - while (true) { - auto alignment = make_shared(); - if (alignment->getChrIndex() > 1000) { - continue; - } - if (alignment->isValidLine()) { - if (alignment->getChrIndex() != chrIndexCurrent) { - switchChromosome(*alignment); + void + SamSegmentMapper::parseSamStream() { + while (true) { + auto alignment = make_shared(); + if (alignment->getChrIndex() > 1000) { + continue; + } + if (alignment->isValidLine()) { + if (alignment->getChrIndex() != chrIndexCurrent) { + switchChromosome(*alignment); + } + alignment->continueConstruction(); + printBps(alignment->getStartPos()); + incrementCoverages(*alignment); + assignBps(alignment); + } else { + break; } - alignment->continueConstruction(); - printBps(alignment->getStartPos()); - incrementCoverages(*alignment); - assignBps(alignment); - } else { - break; } - } - // EOF event for the samtools pipe. printing the end of the very last - // chromosome, - printBps(numeric_limits::max()); -} - -void -SamSegmentMapper::switchChromosome(const Alignment &alignment) { - // As we entered a new chromosome here, now print the previous chromosome's - // unprinted regions - if (chrIndexCurrent != 0) { + // EOF event for the samtools pipe. printing the end of the very last + // chromosome, printBps(numeric_limits::max()); } - chrIndexCurrent = alignment.getChrIndex(); - breakpointsCurrent.clear(); - coverageProfiles.clear(); - discordantAlignmentsPool.clear(); - if (PROPERPARIRCOMPENSATIONMODE) { - discordantAlignmentCandidatesPool.clear(); + + void + SamSegmentMapper::switchChromosome(const Alignment &alignment) { + // As we entered a new chromosome here, now print the previous chromosome's + // unprinted regions + if (chrIndexCurrent != 0) { + printBps(numeric_limits::max()); + } + chrIndexCurrent = alignment.getChrIndex(); + breakpointsCurrent.clear(); + coverageProfiles.clear(); + discordantAlignmentsPool.clear(); + if (PROPERPARIRCOMPENSATIONMODE) { + discordantAlignmentCandidatesPool.clear(); + } + discordantLowQualAlignmentsPool.clear(); + minPos = -1; + maxPos = -1; } - discordantLowQualAlignmentsPool.clear(); - minPos = -1; - maxPos = -1; -} -void -SamSegmentMapper::printBps(int alignmentStart) { - for (auto &bp : breakpointsCurrent) { - if (!bp.second.isCovFinalized() && ((bp.first) + 1 < alignmentStart)) { - auto posDiff = bp.first - minPos; - if (bp.first != minPos) { - bp.second.setLeftCoverage( - coverageProfiles[bp.first - minPos - 1].getCoverage()); - } else { - bp.second.setLeftCoverage(0); + void + SamSegmentMapper::printBps(int alignmentStart) { + for (auto &bp : breakpointsCurrent) { + if (!bp.second.isCovFinalized() && ((bp.first) + 1 < alignmentStart)) { + auto posDiff = bp.first - minPos; + if (bp.first != minPos) { + bp.second.setLeftCoverage( + coverageProfiles[bp.first - minPos - 1].getCoverage()); + } else { + bp.second.setLeftCoverage(0); + } + bp.second.setRightCoverage(coverageProfiles[posDiff].getCoverage()); + bp.second.setNormalSpans( + coverageProfiles[posDiff].getNormalSpans()); + bp.second.setLowQualSpansSoft( + coverageProfiles[posDiff].getLowQualSpansSoft()); + bp.second.setLowQualSpansHard( + coverageProfiles[posDiff].getLowQualSpansHard()); + bp.second.setUnpairedBreaksSoft( + coverageProfiles[posDiff].getNormalBpsSoft()); + bp.second.setUnpairedBreaksHard( + coverageProfiles[posDiff].getNormalBpsHard()); + bp.second.setBreaksShortIndel( + coverageProfiles[posDiff].getNormalBpsShortIndel()); + bp.second.setLowQualBreaksSoft( + coverageProfiles[posDiff].getLowQualBpsSoft()); + bp.second.setLowQualBreaksHard( + coverageProfiles[posDiff].getLowQualBpsHard()); + bp.second.setCovFinalized(true); } - bp.second.setRightCoverage(coverageProfiles[posDiff].getCoverage()); - bp.second.setNormalSpans( - coverageProfiles[posDiff].getNormalSpans()); - bp.second.setLowQualSpansSoft( - coverageProfiles[posDiff].getLowQualSpansSoft()); - bp.second.setLowQualSpansHard( - coverageProfiles[posDiff].getLowQualSpansHard()); - bp.second.setUnpairedBreaksSoft( - coverageProfiles[posDiff].getNormalBpsSoft()); - bp.second.setUnpairedBreaksHard( - coverageProfiles[posDiff].getNormalBpsHard()); - bp.second.setBreaksShortIndel( - coverageProfiles[posDiff].getNormalBpsShortIndel()); - bp.second.setLowQualBreaksSoft( - coverageProfiles[posDiff].getLowQualBpsSoft()); - bp.second.setLowQualBreaksHard( - coverageProfiles[posDiff].getLowQualBpsHard()); - bp.second.setCovFinalized(true); } - } - if (minPos != -1) { - while (minPos + 2 + DISCORDANTLEFTRANGE < alignmentStart) { - if (minPos != maxPos) { - coverageProfiles.pop_front(); - ++minPos; + if (minPos != -1) { + while (minPos + 2 + DISCORDANTLEFTRANGE < alignmentStart) { + if (minPos != maxPos) { + coverageProfiles.pop_front(); + ++minPos; + } else { + coverageProfiles.clear(); + minPos = -1; + maxPos = -1; + break; + } + } + } + for (auto bpIt = breakpointsCurrent.begin(); + bpIt != breakpointsCurrent.end();) { + if ((bpIt->first) + DISCORDANTRIGHTRANGE < alignmentStart) { + if (bpIt->second.finalizeBreakpoint( + discordantAlignmentsPool, discordantLowQualAlignmentsPool, + discordantAlignmentCandidatesPool)) { + ++printedBps; + } + bpIt = breakpointsCurrent.erase(bpIt); } else { - coverageProfiles.clear(); - minPos = -1; - maxPos = -1; break; } } - } - for (auto bpIt = breakpointsCurrent.begin(); - bpIt != breakpointsCurrent.end();) { - if ((bpIt->first) + DISCORDANTRIGHTRANGE < alignmentStart) { - if (bpIt->second.finalizeBreakpoint( - discordantAlignmentsPool, discordantLowQualAlignmentsPool, - discordantAlignmentCandidatesPool)) { - ++printedBps; + while (!discordantAlignmentsPool.empty() && + (discordantAlignmentsPool.front().readStartPos + + DISCORDANTLEFTRANGE + DISCORDANTRIGHTRANGE < + alignmentStart)) { + discordantAlignmentsPool.pop_front(); + } + if (PROPERPARIRCOMPENSATIONMODE) { + while (!discordantAlignmentCandidatesPool.empty() && + (discordantAlignmentCandidatesPool.front().readStartPos + + DISCORDANTLEFTRANGE + DISCORDANTRIGHTRANGE < + alignmentStart)) { + discordantAlignmentCandidatesPool.pop_front(); } - bpIt = breakpointsCurrent.erase(bpIt); - } else { - break; } - } - while (!discordantAlignmentsPool.empty() && - (discordantAlignmentsPool.front().readStartPos + - DISCORDANTLEFTRANGE + DISCORDANTRIGHTRANGE < - alignmentStart)) { - discordantAlignmentsPool.pop_front(); - } - if (PROPERPARIRCOMPENSATIONMODE) { - while (!discordantAlignmentCandidatesPool.empty() && - (discordantAlignmentCandidatesPool.front().readStartPos + + while (!discordantLowQualAlignmentsPool.empty() && + (discordantLowQualAlignmentsPool.front().readStartPos + DISCORDANTLEFTRANGE + DISCORDANTRIGHTRANGE < alignmentStart)) { - discordantAlignmentCandidatesPool.pop_front(); + discordantLowQualAlignmentsPool.pop_front(); } } - while (!discordantLowQualAlignmentsPool.empty() && - (discordantLowQualAlignmentsPool.front().readStartPos + - DISCORDANTLEFTRANGE + DISCORDANTRIGHTRANGE < - alignmentStart)) { - discordantLowQualAlignmentsPool.pop_front(); - } -} -void -SamSegmentMapper::incrementCoverages(const Alignment &alignment) { - if (minPos == -1) { - for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { - coverageProfiles.emplace_back(); - } - minPos = alignment.getStartPos(); - maxPos = alignment.getEndPos() - 1; - } else { - while (alignment.getStartPos() > maxPos) { - coverageProfiles.emplace_back(); - ++maxPos; - } - } - switch (alignment.getReadType()) { - case 0: - case 3: - for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { - if (i > maxPos) { + void + SamSegmentMapper::incrementCoverages(const Alignment &alignment) { + if (minPos == -1) { + for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { coverageProfiles.emplace_back(); - ++maxPos; } - coverageProfiles[i - minPos].incrementCoverage(); - coverageProfiles[i - minPos].incrementNormalSpans(); - } - if (PROPERPARIRCOMPENSATIONMODE) { - discordantAlignmentCandidatesPool.emplace_back( - alignment.getStartPos(), alignment.getEndPos(), -1, -1, -1, - false); - } - break; - case 1: - for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { - if (i > maxPos) { + minPos = alignment.getStartPos(); + maxPos = alignment.getEndPos() - 1; + } else { + while (alignment.getStartPos() > maxPos) { coverageProfiles.emplace_back(); ++maxPos; } - coverageProfiles[i - minPos].incrementCoverage(); - coverageProfiles[i - minPos].incrementNormalSpans(); } - break; - case 4: - for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { - if (i > maxPos) { - coverageProfiles.emplace_back(); - ++maxPos; + switch (alignment.getReadType()) { + case 0: + case 3: + for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { + if (i > maxPos) { + coverageProfiles.emplace_back(); + ++maxPos; + } + coverageProfiles[i - minPos].incrementCoverage(); + coverageProfiles[i - minPos].incrementNormalSpans(); } - coverageProfiles[i - minPos].incrementCoverage(); - coverageProfiles[i - minPos].incrementNormalSpans(); - } - if (alignment.getMateChrIndex() < 1002 && - !(alignment.getMateChrIndex() == 2 && - (alignment.getMatePos() / 10000 == 3314))) { if (PROPERPARIRCOMPENSATIONMODE) { discordantAlignmentCandidatesPool.emplace_back( - alignment.getStartPos(), alignment.getEndPos(), - alignment.getMateChrIndex(), alignment.getMatePos(), 2, - alignment.isInvertedMate()); - } - if (!alignment.isNullMapq()) { - discordantAlignmentsPool.emplace_back( - alignment.getStartPos(), alignment.getEndPos(), - alignment.getMateChrIndex(), alignment.getMatePos(), 2, - alignment.isInvertedMate()); - } else { - discordantLowQualAlignmentsPool.emplace_back( - alignment.getStartPos(), alignment.getEndPos(), - alignment.getMateChrIndex(), alignment.getMatePos(), 2, - alignment.isInvertedMate()); + alignment.getStartPos(), alignment.getEndPos(), -1, -1, -1, + false); } - } - break; - case 2: - if (!(alignment.isLowMapq() || alignment.isNullMapq())) { - for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); - ++i) { + break; + case 1: + for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { if (i > maxPos) { coverageProfiles.emplace_back(); ++maxPos; } + coverageProfiles[i - minPos].incrementCoverage(); + coverageProfiles[i - minPos].incrementNormalSpans(); } - } else { - for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); - ++i) { + break; + case 4: + for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { if (i > maxPos) { coverageProfiles.emplace_back(); ++maxPos; } - coverageProfiles[i - minPos].incrementLowQualSpansHard(); - } - } - break; - case 5: - for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { - if (i > maxPos) { - coverageProfiles.emplace_back(); - ++maxPos; + coverageProfiles[i - minPos].incrementCoverage(); + coverageProfiles[i - minPos].incrementNormalSpans(); } - coverageProfiles[i - minPos].incrementLowQualSpansSoft(); - } - if (!alignment.isSupplementary() && - alignment.getMateChrIndex() < 1002 && alignment.isDistantMate()) { - if (!(alignment.getMateChrIndex() == 2 && + if (alignment.getMateChrIndex() < 1002 && + !(alignment.getMateChrIndex() == 2 && (alignment.getMatePos() / 10000 == 3314))) { - discordantLowQualAlignmentsPool.emplace_back( - alignment.getStartPos(), alignment.getEndPos(), - alignment.getMateChrIndex(), alignment.getMatePos(), 2, - alignment.isInvertedMate(), alignment.getReadBreakpoints()); - } - } - break; - default: - break; - } - switch (alignment.getReadType()) { - case 1: - for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { - auto bpPos = alignment.getReadBreakpoints()[j]; - if (bpPos > maxPos) { - coverageProfiles.emplace_back(); - ++maxPos; + if (PROPERPARIRCOMPENSATIONMODE) { + discordantAlignmentCandidatesPool.emplace_back( + alignment.getStartPos(), alignment.getEndPos(), + alignment.getMateChrIndex(), alignment.getMatePos(), 2, + alignment.isInvertedMate()); + } + if (!alignment.isNullMapq()) { + discordantAlignmentsPool.emplace_back( + alignment.getStartPos(), alignment.getEndPos(), + alignment.getMateChrIndex(), alignment.getMatePos(), 2, + alignment.isInvertedMate()); + } else { + discordantLowQualAlignmentsPool.emplace_back( + alignment.getStartPos(), alignment.getEndPos(), + alignment.getMateChrIndex(), alignment.getMatePos(), 2, + alignment.isInvertedMate()); + } } - switch (alignment.getReadBreakpointTypes()[j]) { - case 'S': - if (bpPos == alignment.getStartPos()) { - coverageProfiles[bpPos - minPos].decrementNormalSpans(); + break; + case 2: + if (!(alignment.isLowMapq() || alignment.isNullMapq())) { + for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); + ++i) { + if (i > maxPos) { + coverageProfiles.emplace_back(); + ++maxPos; + } } - coverageProfiles[bpPos - minPos].incrementNormalBpsSoft(); - break; - case 'I': - coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); - break; - case 'D': - coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); - for (auto k = 0; k != alignment.getReadBreakpointsSizes()[j]; - ++k) { - coverageProfiles[bpPos - minPos + k].decrementNormalSpans(); + } else { + for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); + ++i) { + if (i > maxPos) { + coverageProfiles.emplace_back(); + ++maxPos; + } + coverageProfiles[i - minPos].incrementLowQualSpansHard(); } - break; - default: - break; } - } - break; - case 3: - for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { - auto bpPos = alignment.getReadBreakpoints()[j]; - if (bpPos > maxPos) { - coverageProfiles.emplace_back(); - ++maxPos; + break; + case 5: + for (auto i = alignment.getStartPos(); i < alignment.getEndPos(); ++i) { + if (i > maxPos) { + coverageProfiles.emplace_back(); + ++maxPos; + } + coverageProfiles[i - minPos].incrementLowQualSpansSoft(); } - switch (alignment.getReadBreakpointTypes()[j]) { - case 'I': - coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); - break; - case 'D': - coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); - for (auto k = 0; k != alignment.getReadBreakpointsSizes()[j]; - ++k) { - coverageProfiles[bpPos - minPos + k].decrementNormalSpans(); + if (!alignment.isSupplementary() && + alignment.getMateChrIndex() < 1002 && alignment.isDistantMate()) { + if (!(alignment.getMateChrIndex() == 2 && + (alignment.getMatePos() / 10000 == 3314))) { + discordantLowQualAlignmentsPool.emplace_back( + alignment.getStartPos(), alignment.getEndPos(), + alignment.getMateChrIndex(), alignment.getMatePos(), 2, + alignment.isInvertedMate(), alignment.getReadBreakpoints()); } - break; - default: - break; } + break; + default: + break; } - break; - case 2: - if (!(alignment.isLowMapq() || alignment.isNullMapq())) { + switch (alignment.getReadType()) { + case 1: for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { auto bpPos = alignment.getReadBreakpoints()[j]; if (bpPos > maxPos) { coverageProfiles.emplace_back(); ++maxPos; } - if (alignment.getReadBreakpointTypes()[j] == 'H') { - coverageProfiles[bpPos - minPos].incrementNormalBpsHard(); + switch (alignment.getReadBreakpointTypes()[j]) { + case 'S': + if (bpPos == alignment.getStartPos()) { + coverageProfiles[bpPos - minPos].decrementNormalSpans(); + } + coverageProfiles[bpPos - minPos].incrementNormalBpsSoft(); + break; + case 'I': + coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); + break; + case 'D': + coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); + for (auto k = 0; k != alignment.getReadBreakpointsSizes()[j]; + ++k) { + coverageProfiles[bpPos - minPos + k].decrementNormalSpans(); + } + break; + default: + break; } } - } else { + break; + case 3: for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { auto bpPos = alignment.getReadBreakpoints()[j]; if (bpPos > maxPos) { @@ -355,132 +318,167 @@ SamSegmentMapper::incrementCoverages(const Alignment &alignment) { ++maxPos; } switch (alignment.getReadBreakpointTypes()[j]) { - case 'S': - if (bpPos == alignment.getStartPos()) { - coverageProfiles[bpPos - minPos] - .decrementLowQualSpansHard(); + case 'I': + coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); + break; + case 'D': + coverageProfiles[bpPos - minPos].incrementNormalBpsShortIndel(); + for (auto k = 0; k != alignment.getReadBreakpointsSizes()[j]; + ++k) { + coverageProfiles[bpPos - minPos + k].decrementNormalSpans(); } - coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); break; + default: + break; + } + } + break; + case 2: + if (!(alignment.isLowMapq() || alignment.isNullMapq())) { + for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { + auto bpPos = alignment.getReadBreakpoints()[j]; + if (bpPos > maxPos) { + coverageProfiles.emplace_back(); + ++maxPos; + } + if (alignment.getReadBreakpointTypes()[j] == 'H') { + coverageProfiles[bpPos - minPos].incrementNormalBpsHard(); + } + } + } else { + for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { + auto bpPos = alignment.getReadBreakpoints()[j]; + if (bpPos > maxPos) { + coverageProfiles.emplace_back(); + ++maxPos; + } + switch (alignment.getReadBreakpointTypes()[j]) { + case 'S': + if (bpPos == alignment.getStartPos()) { + coverageProfiles[bpPos - minPos] + .decrementLowQualSpansHard(); + } + coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); + break; + case 'H': + if (bpPos == alignment.getStartPos()) { + coverageProfiles[bpPos - minPos] + .decrementLowQualSpansHard(); + } + coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); + break; + case 'I': + coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); + break; + case 'D': + coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); + for (auto k = 0; + k != alignment.getReadBreakpointsSizes()[j]; ++k) { + coverageProfiles[bpPos - minPos + k] + .decrementLowQualSpansHard(); + } + break; + default: + break; + } + } + } + break; + case 5: + for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { + auto bpPos = alignment.getReadBreakpoints()[j]; + if (bpPos > maxPos) { + coverageProfiles.emplace_back(); + ++maxPos; + } + switch (alignment.getReadBreakpointTypes()[j]) { + case 'S': case 'H': if (bpPos == alignment.getStartPos()) { coverageProfiles[bpPos - minPos] - .decrementLowQualSpansHard(); + .decrementLowQualSpansSoft(); } - coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); + coverageProfiles[bpPos - minPos].incrementLowQualBpsSoft(); break; case 'I': - coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); + coverageProfiles[bpPos - minPos].incrementLowQualBpsSoft(); break; case 'D': - coverageProfiles[bpPos - minPos].incrementLowQualBpsHard(); - for (auto k = 0; - k != alignment.getReadBreakpointsSizes()[j]; ++k) { + coverageProfiles[bpPos - minPos].incrementLowQualBpsSoft(); + for (auto k = 0; k != alignment.getReadBreakpointsSizes()[j]; + ++k) { coverageProfiles[bpPos - minPos + k] - .decrementLowQualSpansHard(); + .decrementLowQualSpansSoft(); } break; default: break; } } + break; + default: + break; } - break; - case 5: - for (auto j = 0u; j < alignment.getReadBreakpoints().size(); ++j) { - auto bpPos = alignment.getReadBreakpoints()[j]; - if (bpPos > maxPos) { - coverageProfiles.emplace_back(); - ++maxPos; - } - switch (alignment.getReadBreakpointTypes()[j]) { - case 'S': - case 'H': - if (bpPos == alignment.getStartPos()) { - coverageProfiles[bpPos - minPos] - .decrementLowQualSpansSoft(); - } - coverageProfiles[bpPos - minPos].incrementLowQualBpsSoft(); - break; - case 'I': - coverageProfiles[bpPos - minPos].incrementLowQualBpsSoft(); - break; - case 'D': - coverageProfiles[bpPos - minPos].incrementLowQualBpsSoft(); - for (auto k = 0; k != alignment.getReadBreakpointsSizes()[j]; - ++k) { - coverageProfiles[bpPos - minPos + k] - .decrementLowQualSpansSoft(); - } - break; - default: - break; - } - } - break; - default: - break; } -} -void -SamSegmentMapper::assignBps(shared_ptr &alignment) { - switch (alignment->getReadType()) { - case 1: - for (auto i = 0u; i < alignment->getReadBreakpoints().size(); ++i) { - if (alignment->getReadBreakpointTypes()[i] == 'S') { - auto bpLoc = alignment->getReadBreakpoints()[i]; - auto it = breakpointsCurrent.find(bpLoc); - if (it == breakpointsCurrent.end()) { - auto newIt = breakpointsCurrent.emplace( - piecewise_construct, forward_as_tuple(bpLoc), - forward_as_tuple(chrIndexCurrent, bpLoc)); - newIt.first->second.addSoftAlignment(alignment); - } else { - it->second.addSoftAlignment(alignment); + void + SamSegmentMapper::assignBps(shared_ptr &alignment) { + switch (alignment->getReadType()) { + case 1: + for (auto i = 0u; i < alignment->getReadBreakpoints().size(); ++i) { + if (alignment->getReadBreakpointTypes()[i] == 'S') { + auto bpLoc = alignment->getReadBreakpoints()[i]; + auto it = breakpointsCurrent.find(bpLoc); + if (it == breakpointsCurrent.end()) { + auto newIt = breakpointsCurrent.emplace( + piecewise_construct, forward_as_tuple(bpLoc), + forward_as_tuple(chrIndexCurrent, bpLoc)); + newIt.first->second.addSoftAlignment(alignment); + } else { + it->second.addSoftAlignment(alignment); + } } } - } - break; - case 2: - for (auto i = 0u; i < alignment->getReadBreakpoints().size(); ++i) { - if (alignment->getReadBreakpointTypes()[i] == 'H') { - auto bpLoc = alignment->getReadBreakpoints()[i]; - auto it = breakpointsCurrent.find(bpLoc); - if (it == breakpointsCurrent.end()) { - auto newIt = breakpointsCurrent.emplace( - piecewise_construct, forward_as_tuple(bpLoc), - forward_as_tuple(chrIndexCurrent, bpLoc)); - newIt.first->second.addHardAlignment(alignment); - } else { - it->second.addHardAlignment(alignment); + break; + case 2: + for (auto i = 0u; i < alignment->getReadBreakpoints().size(); ++i) { + if (alignment->getReadBreakpointTypes()[i] == 'H') { + auto bpLoc = alignment->getReadBreakpoints()[i]; + auto it = breakpointsCurrent.find(bpLoc); + if (it == breakpointsCurrent.end()) { + auto newIt = breakpointsCurrent.emplace( + piecewise_construct, forward_as_tuple(bpLoc), + forward_as_tuple(chrIndexCurrent, bpLoc)); + newIt.first->second.addHardAlignment(alignment); + } else { + it->second.addHardAlignment(alignment); + } } } + break; + default: + break; } - break; - default: - break; } -} -// void SamSegmentMapper::printMetadata(int ISIZESIGMALEVEL) { -// auto elapsedTime = div(difftime(time(nullptr), STARTTIME), 60); -// *metaOutputHandle << "#Using soft/hard clip length threshold " << -//Alignment::CLIPPEDNUCLEOTIDECOUNTTHRESHOLD << endl; *metaOutputHandle << -//"#Using low quality clipped overhang length threshold " << -//Alignment::LOWQUALCLIPTHRESHOLD << endl; *metaOutputHandle << "#Using Base -//Quality Threshold " << Alignment::BASEQUALITYTHRESHOLD << endl; -// *metaOutputHandle << "#Using Base Quality Threshold Low " << -//Alignment::BASEQUALITYTHRESHOLDLOW << endl; *metaOutputHandle << "#Using -//sigmas = " << ISIZESIGMALEVEL << " away from the median insert size for -//'distant' classification" << endl; *metaOutputHandle << "#Using minimum isize -//for 'distant' classification = " << Alignment::ISIZEMAX << " bps" << endl; -// *metaOutputHandle << "#Using minimum reads supporting a breakpoint " << -//Breakpoint::BPSUPPORTTHRESHOLD << endl; *metaOutputHandle << "#Using minimum -//reads supporting a discordant mate contig " << Breakpoint::BPSUPPORTTHRESHOLD -//<< endl; *metaOutputHandle << "#Using (-F 0x600 -f 0x001)" << endl; -// *metaOutputHandle << "#done\t" << printedBps << " lines printed in " << -//elapsedTime.quot << " minutes, " << elapsedTime.rem << " seconds" << endl; -// } + // void SamSegmentMapper::printMetadata(int ISIZESIGMALEVEL) { + // auto elapsedTime = div(difftime(time(nullptr), STARTTIME), 60); + // *metaOutputHandle << "#Using soft/hard clip length threshold " << + //Alignment::CLIPPEDNUCLEOTIDECOUNTTHRESHOLD << endl; *metaOutputHandle << + //"#Using low quality clipped overhang length threshold " << + //Alignment::LOWQUALCLIPTHRESHOLD << endl; *metaOutputHandle << "#Using Base + //Quality Threshold " << Alignment::BASEQUALITYTHRESHOLD << endl; + // *metaOutputHandle << "#Using Base Quality Threshold Low " << + //Alignment::BASEQUALITYTHRESHOLDLOW << endl; *metaOutputHandle << "#Using + //sigmas = " << ISIZESIGMALEVEL << " away from the median insert size for + //'distant' classification" << endl; *metaOutputHandle << "#Using minimum isize + //for 'distant' classification = " << Alignment::ISIZEMAX << " bps" << endl; + // *metaOutputHandle << "#Using minimum reads supporting a breakpoint " << + //Breakpoint::BPSUPPORTTHRESHOLD << endl; *metaOutputHandle << "#Using minimum + //reads supporting a discordant mate contig " << Breakpoint::BPSUPPORTTHRESHOLD + //<< endl; *metaOutputHandle << "#Using (-F 0x600 -f 0x001)" << endl; + // *metaOutputHandle << "#done\t" << printedBps << " lines printed in " << + //elapsedTime.quot << " minutes, " << elapsedTime.rem << " seconds" << endl; + // } } /* namespace sophia */ diff --git a/src/Sdust.cpp b/src/Sdust.cpp index e00c31b..0f58bf2 100644 --- a/src/Sdust.cpp +++ b/src/Sdust.cpp @@ -27,122 +27,120 @@ namespace sophia { -using namespace std; - -Sdust::Sdust(const vector &overhangIn) - : res{}, P{}, w{}, L{0}, rW{0}, rV{0}, cW{vector(WINDOWSIZE, 0)}, - cV{vector(WINDOWSIZE, 0)} { - auto wStart = 0; - for (auto wFinish = 2; wFinish < static_cast(overhangIn.size()); - ++wFinish) { - wStart = max(wFinish - WINDOWSIZE + 1, 0); - saveMaskedRegions(wStart); - auto t = triplet(overhangIn, wFinish - 2); - shiftWindow(t); - if ((rW * 10) > (L * SCORETHRESHOLD)) { - findPerfectRegions(wStart, rV, cV); + Sdust::Sdust(const vector &overhangIn) + : res{}, P{}, w{}, L{0}, rW{0}, rV{0}, cW{vector(WINDOWSIZE, 0)}, + cV{vector(WINDOWSIZE, 0)} { + auto wStart = 0; + for (auto wFinish = 2; wFinish < static_cast(overhangIn.size()); + ++wFinish) { + wStart = max(wFinish - WINDOWSIZE + 1, 0); + saveMaskedRegions(wStart); + auto t = triplet(overhangIn, wFinish - 2); + shiftWindow(t); + if ((rW * 10) > (L * SCORETHRESHOLD)) { + findPerfectRegions(wStart, rV, cV); + } + } + wStart = max(0, static_cast(overhangIn.size()) - WINDOWSIZE + 1); + while (!P.empty()) { + saveMaskedRegions(wStart); + ++wStart; } } - wStart = max(0, static_cast(overhangIn.size()) - WINDOWSIZE + 1); - while (!P.empty()) { - saveMaskedRegions(wStart); - ++wStart; - } -} -void -Sdust::saveMaskedRegions(int wStart) { - if (!P.empty() && P.rbegin()->startIndex < wStart) { - if (!res.empty()) { - auto interval = res.back(); - if (P.rbegin()->startIndex <= (interval.endIndex + 1)) { - res[res.size() - 1].endIndex = - max(P.rbegin()->endIndex, interval.endIndex); + void + Sdust::saveMaskedRegions(int wStart) { + if (!P.empty() && P.rbegin()->startIndex < wStart) { + if (!res.empty()) { + auto interval = res.back(); + if (P.rbegin()->startIndex <= (interval.endIndex + 1)) { + res[res.size() - 1].endIndex = + max(P.rbegin()->endIndex, interval.endIndex); + } else { + res.push_back(PerfectInterval{P.rbegin()->startIndex, + P.rbegin()->endIndex, 0.0}); + } } else { res.push_back(PerfectInterval{P.rbegin()->startIndex, P.rbegin()->endIndex, 0.0}); } - } else { - res.push_back(PerfectInterval{P.rbegin()->startIndex, - P.rbegin()->endIndex, 0.0}); - } - for (;;) { - if (!P.empty() && P.rbegin()->startIndex < wStart) { - P.erase(prev(P.end())); - } else { - break; + for (;;) { + if (!P.empty() && P.rbegin()->startIndex < wStart) { + P.erase(prev(P.end())); + } else { + break; + } } } } -} -void -Sdust::findPerfectRegions(int wStart, int r, vector c) { - auto maxScore = 0.0; - for (auto i = static_cast(w.size()) - L - 1; i >= 0; --i) { - auto t = w[i]; - addTripletInfo(r, c, t); - auto newScore = r / (static_cast(w.size()) - i - 1.0); - if ((newScore * 10) > SCORETHRESHOLD) { - auto cit = P.cbegin(); - while (cit != P.cend()) { - if (cit->startIndex < i + wStart) { - break; + void + Sdust::findPerfectRegions(int wStart, int r, vector c) { + auto maxScore = 0.0; + for (auto i = static_cast(w.size()) - L - 1; i >= 0; --i) { + auto t = w[i]; + addTripletInfo(r, c, t); + auto newScore = r / (static_cast(w.size()) - i - 1.0); + if ((newScore * 10) > SCORETHRESHOLD) { + auto cit = P.cbegin(); + while (cit != P.cend()) { + if (cit->startIndex < i + wStart) { + break; + } + maxScore = max(maxScore, cit->score); + ++cit; + } + if (newScore >= maxScore) { + P.emplace_hint(cit, PerfectInterval{i + wStart, + static_cast(w.size()) + + 1 + wStart, + newScore}); } - maxScore = max(maxScore, cit->score); - ++cit; - } - if (newScore >= maxScore) { - P.emplace_hint(cit, PerfectInterval{i + wStart, - static_cast(w.size()) + - 1 + wStart, - newScore}); } } } -} -void -Sdust::shiftWindow(int t) { - if (w.size() >= WINDOWSIZE - 2) { - auto s = w.front(); - w.pop_front(); - removeTripletInfo(rW, cW, s); - if (L > static_cast(w.size())) { - --L; - removeTripletInfo(rV, cV, s); + void + Sdust::shiftWindow(int t) { + if (w.size() >= WINDOWSIZE - 2) { + auto s = w.front(); + w.pop_front(); + removeTripletInfo(rW, cW, s); + if (L > static_cast(w.size())) { + --L; + removeTripletInfo(rV, cV, s); + } + } + w.push_back(t); + ++L; + addTripletInfo(rW, cW, t); + addTripletInfo(rV, cV, t); + if ((cV[t] * 10) > (SCORETHRESHOLD * 2)) { + int s{0}; + do { + s = w[w.size() - L]; + removeTripletInfo(rV, cV, s); + --L; + } while (s != t); } } - w.push_back(t); - ++L; - addTripletInfo(rW, cW, t); - addTripletInfo(rV, cV, t); - if ((cV[t] * 10) > (SCORETHRESHOLD * 2)) { - int s{0}; - do { - s = w[w.size() - L]; - removeTripletInfo(rV, cV, s); - --L; - } while (s != t); - } -} -void -Sdust::addTripletInfo(int &r, vector &c, int t) { - r += c[t]; - ++c[t]; -} + void + Sdust::addTripletInfo(int &r, vector &c, int t) { + r += c[t]; + ++c[t]; + } -void -Sdust::removeTripletInfo(int &r, vector &c, int t) { - --c[t]; - r -= c[t]; -} + void + Sdust::removeTripletInfo(int &r, vector &c, int t) { + --c[t]; + r -= c[t]; + } -int -Sdust::triplet(const vector &overhangIn, int indexPos) { - return 16 * overhangIn[indexPos] + 4 * overhangIn[indexPos + 1] + - overhangIn[indexPos + 2]; -} + int + Sdust::triplet(const vector &overhangIn, int indexPos) { + return 16 * overhangIn[indexPos] + 4 * overhangIn[indexPos + 1] + + overhangIn[indexPos + 2]; + } } /* namespace sophia */ diff --git a/src/SuppAlignment.cpp b/src/SuppAlignment.cpp index 099d37a..a633239 100644 --- a/src/SuppAlignment.cpp +++ b/src/SuppAlignment.cpp @@ -30,379 +30,377 @@ namespace sophia { -using namespace std; + double SuppAlignment::ISIZEMAX { }; -double SuppAlignment::ISIZEMAX { }; + int SuppAlignment::DEFAULTREADLENGTH { }; -int SuppAlignment::DEFAULTREADLENGTH { }; + SuppAlignment::SuppAlignment(string::const_iterator saCbegin, + string::const_iterator saCend, + bool primaryIn, + bool lowMapqSourceIn, + bool nullMapqSourceIn, + bool alignmentOnForwardStrand, + bool bpEncounteredM, + int originIndexIn, + int bpChrIndex, + int bpPos) : + matchFuzziness { 5 * DEFAULTREADLENGTH }, + chrIndex { 0 }, + pos { 0 }, + extendedPos { 0 }, + mapq { 0 }, + supportingIndices { }, + supportingIndicesSecondary { }, + distinctReads { 1 }, + support { 0 }, + secondarySupport { 0 }, + mateSupport { 0 }, + expectedDiscordants { 0 }, + encounteredM { bpEncounteredM }, + toRemove { false }, + inverted { false }, + fuzzy { false }, + strictFuzzy { false }, + distant { false }, + lowMapqSource { lowMapqSourceIn }, + nullMapqSource { nullMapqSourceIn }, + suspicious { false }, + semiSuspicious { false }, + properPairErrorProne { false }, + primary { primaryIn } { + if (primary) { + supportingIndices.push_back(originIndexIn); + } else { + supportingIndicesSecondary.push_back(originIndexIn); + } + // //"SA:Z:10,24753146,+,68S33M,48,1;X,135742083,-,47S22M32S,0,0;8,72637925,-,29S19M53S,0,0;" + // //"10,24753146,+,68S33M,48,1" + // for (auto cigarString_cit = saCbegin; cigarString_cit != saCend; ++cigarString_cit) { + // cerr << *cigarString_cit; + // } + // cerr << endl; -SuppAlignment::SuppAlignment(string::const_iterator saCbegin, - string::const_iterator saCend, - bool primaryIn, - bool lowMapqSourceIn, - bool nullMapqSourceIn, - bool alignmentOnForwardStrand, - bool bpEncounteredM, - int originIndexIn, - int bpChrIndex, - int bpPos) : - matchFuzziness { 5 * DEFAULTREADLENGTH }, - chrIndex { 0 }, - pos { 0 }, - extendedPos { 0 }, - mapq { 0 }, - supportingIndices { }, - supportingIndicesSecondary { }, - distinctReads { 1 }, - support { 0 }, - secondarySupport { 0 }, - mateSupport { 0 }, - expectedDiscordants { 0 }, - encounteredM { bpEncounteredM }, - toRemove { false }, - inverted { false }, - fuzzy { false }, - strictFuzzy { false }, - distant { false }, - lowMapqSource { lowMapqSourceIn }, - nullMapqSource { nullMapqSourceIn }, - suspicious { false }, - semiSuspicious { false }, - properPairErrorProne { false }, - primary { primaryIn } { - if (primary) { - supportingIndices.push_back(originIndexIn); - } else { - supportingIndicesSecondary.push_back(originIndexIn); - } -// //"SA:Z:10,24753146,+,68S33M,48,1;X,135742083,-,47S22M32S,0,0;8,72637925,-,29S19M53S,0,0;" -// //"10,24753146,+,68S33M,48,1" -// for (auto cigarString_cit = saCbegin; cigarString_cit != saCend; ++cigarString_cit) { -// cerr << *cigarString_cit; -// } -// cerr << endl; + vector fieldBegins = { saCbegin }; + vector fieldEnds; + for (auto it = saCbegin; it != saCend; ++it) { + if (*it == ',') { + fieldEnds.push_back(it); + fieldBegins.push_back(it + 1); + } + } + fieldEnds.push_back(saCend); - vector fieldBegins = { saCbegin }; - vector fieldEnds; - for (auto it = saCbegin; it != saCend; ++it) { - if (*it == ',') { - fieldEnds.push_back(it); - fieldBegins.push_back(it + 1); - } - } - fieldEnds.push_back(saCend); + chrIndex = GlobalAppConfig::getInstance(). + getChrConverter().readChromosomeIndex(fieldBegins[0], ','); + if (chrIndex > 1001) { + return; + } + for (auto it = fieldBegins[1]; it != fieldEnds[1]; ++it) { + pos = 10 * pos + (*it - '0'); + } - chrIndex = GlobalAppConfig::getInstance(). - getChrConverter().readChromosomeIndex(fieldBegins[0], ','); - if (chrIndex > 1001) { - return; - } - for (auto it = fieldBegins[1]; it != fieldEnds[1]; ++it) { - pos = 10 * pos + (*it - '0'); - } + //cerr << "guessSupplementOffset" << endl; + vector cigarChunks; + auto cigarEncounteredM = false; + auto cumulativeNucleotideCount = 0, + currentNucleotideCount = 0, + chunkIndex = 0, + bestChunkIndex = 0, + indelAdjustment = 0; + auto largestClip = 0; + auto leftClipAdjustment = 0; + for (auto cigarString_cit = fieldBegins[3]; cigarString_cit != fieldEnds[3]; ++cigarString_cit) { + if (isdigit(*cigarString_cit)) { + currentNucleotideCount = currentNucleotideCount * 10 + (*cigarString_cit - '0'); + } else { + switch (*cigarString_cit) { + case 'M': + cigarEncounteredM = true; + cumulativeNucleotideCount += currentNucleotideCount; + break; + case 'S': + if (!cigarEncounteredM) { + leftClipAdjustment = currentNucleotideCount; + } + cigarChunks.emplace_back(*cigarString_cit, + cigarEncounteredM, + cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, + currentNucleotideCount); + if (largestClip < currentNucleotideCount) { + largestClip = currentNucleotideCount; + bestChunkIndex = chunkIndex; + } + ++chunkIndex; + cumulativeNucleotideCount += currentNucleotideCount; + break; + case 'H': + cigarChunks.emplace_back(*cigarString_cit, + cigarEncounteredM, + cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, + currentNucleotideCount); + if (largestClip < currentNucleotideCount) { + largestClip = currentNucleotideCount; + bestChunkIndex = chunkIndex; + } + ++chunkIndex; + break; + case 'I': + indelAdjustment -= currentNucleotideCount; + cumulativeNucleotideCount += currentNucleotideCount; + break; + case 'D': + indelAdjustment += currentNucleotideCount; + break; + default: + break; + } + currentNucleotideCount = 0; + } + } + if (cigarChunks[bestChunkIndex].encounteredM) { + pos += cigarChunks[bestChunkIndex].startPosOnRead; + } + extendedPos = pos; + //cerr << "done" << endl; + for (auto it = fieldBegins[4]; it != fieldEnds[4]; ++it) { + mapq = 10 * mapq + (*it - '0'); + } + if (alignmentOnForwardStrand) { + inverted = ('+' != *fieldBegins[2]); + } else { + inverted = ('-' != *fieldBegins[2]); + } + distant = (bpChrIndex != chrIndex || (abs(bpPos - pos) > ISIZEMAX)); + if (bpChrIndex == chrIndex) { + matchFuzziness = min(abs(bpPos - pos), matchFuzziness); + } + strictFuzzy = fuzzy || (support + secondarySupport) < 3; + } - //cerr << "guessSupplementOffset" << endl; - vector cigarChunks; - auto cigarEncounteredM = false; - auto cumulativeNucleotideCount = 0, - currentNucleotideCount = 0, - chunkIndex = 0, - bestChunkIndex = 0, - indelAdjustment = 0; - auto largestClip = 0; - auto leftClipAdjustment = 0; - for (auto cigarString_cit = fieldBegins[3]; cigarString_cit != fieldEnds[3]; ++cigarString_cit) { - if (isdigit(*cigarString_cit)) { - currentNucleotideCount = currentNucleotideCount * 10 + (*cigarString_cit - '0'); - } else { - switch (*cigarString_cit) { - case 'M': - cigarEncounteredM = true; - cumulativeNucleotideCount += currentNucleotideCount; - break; - case 'S': - if (!cigarEncounteredM) { - leftClipAdjustment = currentNucleotideCount; - } - cigarChunks.emplace_back(*cigarString_cit, - cigarEncounteredM, - cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, - currentNucleotideCount); - if (largestClip < currentNucleotideCount) { - largestClip = currentNucleotideCount; - bestChunkIndex = chunkIndex; - } - ++chunkIndex; - cumulativeNucleotideCount += currentNucleotideCount; - break; - case 'H': - cigarChunks.emplace_back(*cigarString_cit, - cigarEncounteredM, - cumulativeNucleotideCount + indelAdjustment - leftClipAdjustment, - currentNucleotideCount); - if (largestClip < currentNucleotideCount) { - largestClip = currentNucleotideCount; - bestChunkIndex = chunkIndex; - } - ++chunkIndex; - break; - case 'I': - indelAdjustment -= currentNucleotideCount; - cumulativeNucleotideCount += currentNucleotideCount; - break; - case 'D': - indelAdjustment += currentNucleotideCount; - break; - default: - break; - } - currentNucleotideCount = 0; - } - } - if (cigarChunks[bestChunkIndex].encounteredM) { - pos += cigarChunks[bestChunkIndex].startPosOnRead; - } - extendedPos = pos; - //cerr << "done" << endl; - for (auto it = fieldBegins[4]; it != fieldEnds[4]; ++it) { - mapq = 10 * mapq + (*it - '0'); - } - if (alignmentOnForwardStrand) { - inverted = ('+' != *fieldBegins[2]); - } else { - inverted = ('-' != *fieldBegins[2]); - } - distant = (bpChrIndex != chrIndex || (abs(bpPos - pos) > ISIZEMAX)); - if (bpChrIndex == chrIndex) { - matchFuzziness = min(abs(bpPos - pos), matchFuzziness); - } - strictFuzzy = fuzzy || (support + secondarySupport) < 3; -} + void SuppAlignment::finalizeSupportingIndices() { + sort(supportingIndices.begin(), supportingIndices.end()); + sort(supportingIndicesSecondary.begin(), supportingIndicesSecondary.end()); + supportingIndices.erase(unique(supportingIndices.begin(), supportingIndices.end()), + supportingIndices.end()); + supportingIndicesSecondary.erase(unique(supportingIndicesSecondary.begin(), + supportingIndicesSecondary.end()), + supportingIndicesSecondary.end()); + support = static_cast(supportingIndices.size()); + secondarySupport = static_cast(supportingIndicesSecondary.size()); + } -void SuppAlignment::finalizeSupportingIndices() { - sort(supportingIndices.begin(), supportingIndices.end()); - sort(supportingIndicesSecondary.begin(), supportingIndicesSecondary.end()); - supportingIndices.erase(unique(supportingIndices.begin(), supportingIndices.end()), - supportingIndices.end()); - supportingIndicesSecondary.erase(unique(supportingIndicesSecondary.begin(), - supportingIndicesSecondary.end()), - supportingIndicesSecondary.end()); - support = static_cast(supportingIndices.size()); - secondarySupport = static_cast(supportingIndicesSecondary.size()); -} + SuppAlignment::SuppAlignment(int chrIndexIn, + int posIn, + int mateSupportIn, + int expectedDiscordantsIn, + bool encounteredMIn, + bool invertedIn, + int extendedPosIn, + bool primaryIn, + bool lowMapqSourceIn, + bool nullMapqSourceIn, + int originIndexIn) : + matchFuzziness { 5 * DEFAULTREADLENGTH }, + chrIndex { chrIndexIn }, + pos { posIn }, + extendedPos { extendedPosIn }, + mapq { 0 }, + supportingIndices { }, + supportingIndicesSecondary { }, + distinctReads { 1 }, + support { 0 }, + secondarySupport { 0 }, + mateSupport { mateSupportIn }, + expectedDiscordants { expectedDiscordantsIn }, + encounteredM { encounteredMIn }, + toRemove { false }, + inverted { invertedIn }, + fuzzy { extendedPosIn != posIn }, + strictFuzzy { false }, + distant { true }, + lowMapqSource { lowMapqSourceIn }, + nullMapqSource { nullMapqSourceIn }, + suspicious { false }, + semiSuspicious { false }, + properPairErrorProne { false }, + primary { primaryIn } { + if (originIndexIn != -1) { + if (primary) { + supportingIndices.push_back(originIndexIn); + } else { + supportingIndicesSecondary.push_back(originIndexIn); + } + } else { + distinctReads = 0; + } + strictFuzzy = fuzzy || (support + secondarySupport) < 3; + } -SuppAlignment::SuppAlignment(int chrIndexIn, - int posIn, - int mateSupportIn, - int expectedDiscordantsIn, - bool encounteredMIn, - bool invertedIn, - int extendedPosIn, - bool primaryIn, - bool lowMapqSourceIn, - bool nullMapqSourceIn, - int originIndexIn) : - matchFuzziness { 5 * DEFAULTREADLENGTH }, - chrIndex { chrIndexIn }, - pos { posIn }, - extendedPos { extendedPosIn }, - mapq { 0 }, - supportingIndices { }, - supportingIndicesSecondary { }, - distinctReads { 1 }, - support { 0 }, - secondarySupport { 0 }, - mateSupport { mateSupportIn }, - expectedDiscordants { expectedDiscordantsIn }, - encounteredM { encounteredMIn }, - toRemove { false }, - inverted { invertedIn }, - fuzzy { extendedPosIn != posIn }, - strictFuzzy { false }, - distant { true }, - lowMapqSource { lowMapqSourceIn }, - nullMapqSource { nullMapqSourceIn }, - suspicious { false }, - semiSuspicious { false }, - properPairErrorProne { false }, - primary { primaryIn } { - if (originIndexIn != -1) { - if (primary) { - supportingIndices.push_back(originIndexIn); - } else { - supportingIndicesSecondary.push_back(originIndexIn); - } - } else { - distinctReads = 0; - } - strictFuzzy = fuzzy || (support + secondarySupport) < 3; -} + string SuppAlignment::print() const { + string outStr; + outStr.reserve(36); + string invStr { }; + if (inverted) { + invStr.append("_INV"); + } + if (encounteredM) { + outStr.append("|"); + } else { + invStr.append("|"); + } + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + if (!fuzzy) { + outStr. + append(chrConverter.indexToChr[chrIndex]). + append(":"). + append(strtk::type_to_string(pos)); + } else { + outStr. + append(chrConverter.indexToChr[chrIndex]). + append(":"). + append(strtk::type_to_string(pos)). + append("-"). + append(strtk::type_to_string(extendedPos)); + } + outStr. + append(invStr). + append("("). + append(strtk::type_to_string(support)). + append(","). + append(strtk::type_to_string(secondarySupport)). + append(","); + if (!suspicious) { + outStr.append(strtk::type_to_string(mateSupport)); + if (semiSuspicious || nullMapqSource) { + outStr.append("?"); + } + } else { + outStr.append("!"); + } + outStr.append("/").append(strtk::type_to_string(expectedDiscordants)).append(")"); + if (properPairErrorProne) { + outStr.append("#"); + } + return outStr; + } -string SuppAlignment::print() const { - string outStr; - outStr.reserve(36); - string invStr { }; - if (inverted) { - invStr.append("_INV"); - } - if (encounteredM) { - outStr.append("|"); - } else { - invStr.append("|"); - } - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - if (!fuzzy) { - outStr. - append(chrConverter.indexToChr[chrIndex]). - append(":"). - append(strtk::type_to_string(pos)); - } else { - outStr. - append(chrConverter.indexToChr[chrIndex]). - append(":"). - append(strtk::type_to_string(pos)). - append("-"). - append(strtk::type_to_string(extendedPos)); - } - outStr. - append(invStr). - append("("). - append(strtk::type_to_string(support)). - append(","). - append(strtk::type_to_string(secondarySupport)). - append(","); - if (!suspicious) { - outStr.append(strtk::type_to_string(mateSupport)); - if (semiSuspicious || nullMapqSource) { - outStr.append("?"); - } - } else { - outStr.append("!"); - } - outStr.append("/").append(strtk::type_to_string(expectedDiscordants)).append(")"); - if (properPairErrorProne) { - outStr.append("#"); - } - return outStr; -} + SuppAlignment::SuppAlignment(const string& saIn) : + matchFuzziness { 5 * DEFAULTREADLENGTH }, + chrIndex { 0 }, + pos { 0 }, + extendedPos { 0 }, + mapq { 0 }, + distinctReads { 1 }, + support { 0 }, + secondarySupport { 0 }, + mateSupport { 0 }, + expectedDiscordants { 0 }, + encounteredM { saIn[0] == '|' }, + toRemove { false }, + inverted { false }, + fuzzy { false }, + strictFuzzy { false }, + distant { false }, + lowMapqSource { false }, + nullMapqSource { false }, + suspicious { false }, + semiSuspicious { false }, + properPairErrorProne { saIn.back() == '#' }, + primary { true } { + auto index = 0; + if (encounteredM) { + ++index; + } + chrIndex = GlobalAppConfig::getInstance(). + getChrConverter().readChromosomeIndex(next(saIn.cbegin(), index), ':'); + if (chrIndex > 1001) { + return; + } + while (saIn[index] != ':') { + ++index; + } + ++index; + for (; saIn[index] != '('; ++index) { + if (saIn[index] == '-') { + fuzzy = true; + } else if (saIn[index] == '_') { + inverted = true; + while (saIn[index] != '(') { + ++index; + } + break; + } else if (saIn[index] != '|') { + if (!fuzzy) { + pos = 10 * pos + (saIn[index] - '0'); + } else { + extendedPos = 10 * extendedPos + (saIn[index] - '0'); + } + } + } + if (!fuzzy) { + extendedPos = pos; + } + ++index; + for (; saIn[index] != ','; ++index) { + support = 10 * support + (saIn[index] - '0'); + } + ++index; + for (; saIn[index] != ','; ++index) { + secondarySupport = 10 * secondarySupport + (saIn[index] - '0'); + } + ++index; + if (saIn[index] == '!') { + suspicious = true; + index += 2; + } else { + for (; saIn[index] != '/'; ++index) { + if (saIn[index] == '?') { + semiSuspicious = true; + } else { + mateSupport = 10 * mateSupport + (saIn[index] - '0'); + } + } + ++index; + } + for (; saIn[index] != ')'; ++index) { + expectedDiscordants = 10 * expectedDiscordants + (saIn[index] - '0'); + } + distant = expectedDiscordants > 0 || suspicious; + strictFuzzy = fuzzy || (support + secondarySupport) < 3; + } -SuppAlignment::SuppAlignment(const string& saIn) : - matchFuzziness { 5 * DEFAULTREADLENGTH }, - chrIndex { 0 }, - pos { 0 }, - extendedPos { 0 }, - mapq { 0 }, - distinctReads { 1 }, - support { 0 }, - secondarySupport { 0 }, - mateSupport { 0 }, - expectedDiscordants { 0 }, - encounteredM { saIn[0] == '|' }, - toRemove { false }, - inverted { false }, - fuzzy { false }, - strictFuzzy { false }, - distant { false }, - lowMapqSource { false }, - nullMapqSource { false }, - suspicious { false }, - semiSuspicious { false }, - properPairErrorProne { saIn.back() == '#' }, - primary { true } { - auto index = 0; - if (encounteredM) { - ++index; - } - chrIndex = GlobalAppConfig::getInstance(). - getChrConverter().readChromosomeIndex(next(saIn.cbegin(), index), ':'); - if (chrIndex > 1001) { - return; - } - while (saIn[index] != ':') { - ++index; - } - ++index; - for (; saIn[index] != '('; ++index) { - if (saIn[index] == '-') { - fuzzy = true; - } else if (saIn[index] == '_') { - inverted = true; - while (saIn[index] != '(') { - ++index; - } - break; - } else if (saIn[index] != '|') { - if (!fuzzy) { - pos = 10 * pos + (saIn[index] - '0'); - } else { - extendedPos = 10 * extendedPos + (saIn[index] - '0'); - } - } - } - if (!fuzzy) { - extendedPos = pos; - } - ++index; - for (; saIn[index] != ','; ++index) { - support = 10 * support + (saIn[index] - '0'); - } - ++index; - for (; saIn[index] != ','; ++index) { - secondarySupport = 10 * secondarySupport + (saIn[index] - '0'); - } - ++index; - if (saIn[index] == '!') { - suspicious = true; - index += 2; - } else { - for (; saIn[index] != '/'; ++index) { - if (saIn[index] == '?') { - semiSuspicious = true; - } else { - mateSupport = 10 * mateSupport + (saIn[index] - '0'); - } - } - ++index; - } - for (; saIn[index] != ')'; ++index) { - expectedDiscordants = 10 * expectedDiscordants + (saIn[index] - '0'); - } - distant = expectedDiscordants > 0 || suspicious; - strictFuzzy = fuzzy || (support + secondarySupport) < 3; -} + bool SuppAlignment::saCloseness(const SuppAlignment& rhs, int fuzziness) const { + if (inverted == rhs.isInverted() + && chrIndex == rhs.getChrIndex() + && encounteredM == rhs.isEncounteredM()) { + if (strictFuzzy || rhs.isStrictFuzzy()) { + fuzziness = 2.5 * DEFAULTREADLENGTH; + return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && + (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); + } else { + return abs(pos - rhs.getPos()) <= fuzziness; + } + } else { + return false; + } + } -bool SuppAlignment::saCloseness(const SuppAlignment& rhs, int fuzziness) const { - if (inverted == rhs.isInverted() - && chrIndex == rhs.getChrIndex() - && encounteredM == rhs.isEncounteredM()) { - if (strictFuzzy || rhs.isStrictFuzzy()) { - fuzziness = 2.5 * DEFAULTREADLENGTH; - return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && - (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); - } else { - return abs(pos - rhs.getPos()) <= fuzziness; - } - } else { - return false; - } -} - -bool SuppAlignment::saDistHomologyRescueCloseness(const SuppAlignment& rhs, int fuzziness) const { - if (!distant || !rhs.isDistant()) { - return false; - } - if (chrIndex == rhs.getChrIndex() && encounteredM == rhs.isEncounteredM()) { - if (strictFuzzy || rhs.isStrictFuzzy()) { - return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && - (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); - } else { - return abs(pos - rhs.getPos()) <= fuzziness; - } - } else { - return false; - } -} + bool SuppAlignment::saDistHomologyRescueCloseness(const SuppAlignment& rhs, int fuzziness) const { + if (!distant || !rhs.isDistant()) { + return false; + } + if (chrIndex == rhs.getChrIndex() && encounteredM == rhs.isEncounteredM()) { + if (strictFuzzy || rhs.isStrictFuzzy()) { + return (rhs.getPos() - fuzziness) <= (extendedPos + fuzziness) && + (pos - fuzziness) <= (rhs.getExtendedPos() + fuzziness); + } else { + return abs(pos - rhs.getPos()) <= fuzziness; + } + } else { + return false; + } + } }/* namespace sophia */ diff --git a/src/SuppAlignmentAnno.cpp b/src/SuppAlignmentAnno.cpp index 9794157..66e4202 100644 --- a/src/SuppAlignmentAnno.cpp +++ b/src/SuppAlignmentAnno.cpp @@ -29,273 +29,270 @@ #include #include #include -// #include namespace sophia { -using namespace std; + double SuppAlignmentAnno::ISIZEMAX{}; + int SuppAlignmentAnno::DEFAULTREADLENGTH{}; -double SuppAlignmentAnno::ISIZEMAX{}; -int SuppAlignmentAnno::DEFAULTREADLENGTH{}; - -SuppAlignmentAnno::SuppAlignmentAnno(const string &saStrIn) - : chrIndex{0}, pos{0}, extendedPos{0}, support{0}, secondarySupport{0}, - mateSupport{0}, expectedDiscordants{0}, encounteredM{saStrIn[0] == '|'}, - toRemove{false}, inverted{false}, fuzzy{false}, strictFuzzy{false}, - strictFuzzyCandidate{false}, distant{false}, suspicious{false}, - semiSuspicious{false}, properPairErrorProne{saStrIn.back() == '#'}, - supportingIndices{} { - auto index = 0; - if (encounteredM) { - ++index; - } - chrIndex = - GlobalAppConfig::getInstance(). - getChrConverter().readChromosomeIndex(next(saStrIn.cbegin(), index), ':'); - if (chrIndex > 1001) { - return; - } - while (saStrIn[index] != ':') { + SuppAlignmentAnno::SuppAlignmentAnno(const string &saStrIn) + : chrIndex{0}, pos{0}, extendedPos{0}, support{0}, secondarySupport{0}, + mateSupport{0}, expectedDiscordants{0}, encounteredM{saStrIn[0] == '|'}, + toRemove{false}, inverted{false}, fuzzy{false}, strictFuzzy{false}, + strictFuzzyCandidate{false}, distant{false}, suspicious{false}, + semiSuspicious{false}, properPairErrorProne{saStrIn.back() == '#'}, + supportingIndices{} { + auto index = 0; + if (encounteredM) { + ++index; + } + chrIndex = + GlobalAppConfig::getInstance(). + getChrConverter().readChromosomeIndex(next(saStrIn.cbegin(), index), ':'); + if (chrIndex > 1001) { + return; + } + while (saStrIn[index] != ':') { + ++index; + } ++index; - } - ++index; - for (; saStrIn[index] != '('; ++index) { - if (saStrIn[index] == '-') { - fuzzy = true; - } else if (saStrIn[index] == '_') { - inverted = true; - while (saStrIn[index] != '(') { - ++index; - } - break; - } else if (saStrIn[index] != '|') { - if (!fuzzy) { - pos = 10 * pos + (saStrIn[index] - '0'); - } else { - extendedPos = 10 * extendedPos + (saStrIn[index] - '0'); + for (; saStrIn[index] != '('; ++index) { + if (saStrIn[index] == '-') { + fuzzy = true; + } else if (saStrIn[index] == '_') { + inverted = true; + while (saStrIn[index] != '(') { + ++index; + } + break; + } else if (saStrIn[index] != '|') { + if (!fuzzy) { + pos = 10 * pos + (saStrIn[index] - '0'); + } else { + extendedPos = 10 * extendedPos + (saStrIn[index] - '0'); + } } } - } - if (!fuzzy) { - extendedPos = pos; - } - ++index; - for (; saStrIn[index] != ','; ++index) { - support = 10 * support + (saStrIn[index] - '0'); - } - ++index; - for (; saStrIn[index] != ','; ++index) { - secondarySupport = 10 * secondarySupport + (saStrIn[index] - '0'); - } - ++index; - if (saStrIn[index] == '!') { - suspicious = true; - index += 2; - } else { - for (; saStrIn[index] != '/'; ++index) { - if (saStrIn[index] == '?') { - semiSuspicious = true; - } else { - mateSupport = 10 * mateSupport + (saStrIn[index] - '0'); - } + if (!fuzzy) { + extendedPos = pos; } ++index; + for (; saStrIn[index] != ','; ++index) { + support = 10 * support + (saStrIn[index] - '0'); + } + ++index; + for (; saStrIn[index] != ','; ++index) { + secondarySupport = 10 * secondarySupport + (saStrIn[index] - '0'); + } + ++index; + if (saStrIn[index] == '!') { + suspicious = true; + index += 2; + } else { + for (; saStrIn[index] != '/'; ++index) { + if (saStrIn[index] == '?') { + semiSuspicious = true; + } else { + mateSupport = 10 * mateSupport + (saStrIn[index] - '0'); + } + } + ++index; + } + for (; saStrIn[index] != ')'; ++index) { + expectedDiscordants = 10 * expectedDiscordants + (saStrIn[index] - '0'); + } + if (support + secondarySupport == 0) { + fuzzy = true; + } + distant = expectedDiscordants > 0 || suspicious; + strictFuzzyCandidate = (support + secondarySupport) < 3; + strictFuzzy = fuzzy || strictFuzzyCandidate; } - for (; saStrIn[index] != ')'; ++index) { - expectedDiscordants = 10 * expectedDiscordants + (saStrIn[index] - '0'); - } - if (support + secondarySupport == 0) { - fuzzy = true; - } - distant = expectedDiscordants > 0 || suspicious; - strictFuzzyCandidate = (support + secondarySupport) < 3; - strictFuzzy = fuzzy || strictFuzzyCandidate; -} -SuppAlignmentAnno::SuppAlignmentAnno(const SuppAlignment &saIn) - : chrIndex{saIn.getChrIndex()}, pos{saIn.getPos()}, - extendedPos{saIn.getExtendedPos()}, support{saIn.getSupport()}, - secondarySupport{saIn.getSecondarySupport()}, - mateSupport{saIn.getMateSupport()}, - expectedDiscordants{saIn.getExpectedDiscordants()}, - encounteredM{saIn.isEncounteredM()}, toRemove{false}, - inverted{saIn.isInverted()}, fuzzy{saIn.isFuzzy()}, strictFuzzy{false}, - strictFuzzyCandidate{false}, distant{false}, - suspicious{saIn.isSuspicious()}, semiSuspicious{saIn.isSemiSuspicious()}, - properPairErrorProne{saIn.isProperPairErrorProne()}, - supportingIndices{saIn.getSupportingIndices()} { - distant = expectedDiscordants > 0 || suspicious; - if (support + secondarySupport == 0) { - fuzzy = true; + SuppAlignmentAnno::SuppAlignmentAnno(const SuppAlignment &saIn) + : chrIndex{saIn.getChrIndex()}, pos{saIn.getPos()}, + extendedPos{saIn.getExtendedPos()}, support{saIn.getSupport()}, + secondarySupport{saIn.getSecondarySupport()}, + mateSupport{saIn.getMateSupport()}, + expectedDiscordants{saIn.getExpectedDiscordants()}, + encounteredM{saIn.isEncounteredM()}, toRemove{false}, + inverted{saIn.isInverted()}, fuzzy{saIn.isFuzzy()}, strictFuzzy{false}, + strictFuzzyCandidate{false}, distant{false}, + suspicious{saIn.isSuspicious()}, semiSuspicious{saIn.isSemiSuspicious()}, + properPairErrorProne{saIn.isProperPairErrorProne()}, + supportingIndices{saIn.getSupportingIndices()} { + distant = expectedDiscordants > 0 || suspicious; + if (support + secondarySupport == 0) { + fuzzy = true; + } + strictFuzzyCandidate = (support + secondarySupport) < 3; + strictFuzzy = fuzzy || strictFuzzyCandidate; } - strictFuzzyCandidate = (support + secondarySupport) < 3; - strictFuzzy = fuzzy || strictFuzzyCandidate; -} -SuppAlignmentAnno::SuppAlignmentAnno(const SuppAlignmentAnno &saAnnoIn) - : chrIndex{saAnnoIn.getChrIndex()}, pos{saAnnoIn.getPos()}, - extendedPos{saAnnoIn.getExtendedPos()}, support{saAnnoIn.getSupport()}, - secondarySupport{saAnnoIn.getSecondarySupport()}, - mateSupport{saAnnoIn.getMateSupport()}, - expectedDiscordants{saAnnoIn.getExpectedDiscordants()}, - encounteredM{saAnnoIn.isEncounteredM()}, toRemove{false}, - inverted{saAnnoIn.isInverted()}, fuzzy{saAnnoIn.isFuzzy()}, - strictFuzzy{saAnnoIn.isStrictFuzzy()}, - strictFuzzyCandidate{saAnnoIn.isStrictFuzzyCandidate()}, - distant{saAnnoIn.isDistant()}, suspicious{saAnnoIn.isSuspicious()}, - semiSuspicious{saAnnoIn.isSemiSuspicious()}, - properPairErrorProne{saAnnoIn.isProperPairErrorProne()}, - supportingIndices{saAnnoIn.getSupportingIndices()} {} + SuppAlignmentAnno::SuppAlignmentAnno(const SuppAlignmentAnno &saAnnoIn) + : chrIndex{saAnnoIn.getChrIndex()}, pos{saAnnoIn.getPos()}, + extendedPos{saAnnoIn.getExtendedPos()}, support{saAnnoIn.getSupport()}, + secondarySupport{saAnnoIn.getSecondarySupport()}, + mateSupport{saAnnoIn.getMateSupport()}, + expectedDiscordants{saAnnoIn.getExpectedDiscordants()}, + encounteredM{saAnnoIn.isEncounteredM()}, toRemove{false}, + inverted{saAnnoIn.isInverted()}, fuzzy{saAnnoIn.isFuzzy()}, + strictFuzzy{saAnnoIn.isStrictFuzzy()}, + strictFuzzyCandidate{saAnnoIn.isStrictFuzzyCandidate()}, + distant{saAnnoIn.isDistant()}, suspicious{saAnnoIn.isSuspicious()}, + semiSuspicious{saAnnoIn.isSemiSuspicious()}, + properPairErrorProne{saAnnoIn.isProperPairErrorProne()}, + supportingIndices{saAnnoIn.getSupportingIndices()} {} -SuppAlignmentAnno::SuppAlignmentAnno(int emittingBpChrIndex, int emittingBpPos, - const SuppAlignmentAnno &saAnnoIn) - : chrIndex{emittingBpChrIndex}, - pos{saAnnoIn.isDistant() - ? max(1, static_cast( - round(emittingBpPos - 1.5 * DEFAULTREADLENGTH))) - : emittingBpPos}, - extendedPos{ - saAnnoIn.isDistant() - ? static_cast(emittingBpPos + 1.5 * DEFAULTREADLENGTH) - : emittingBpPos}, - support{0}, secondarySupport{0}, mateSupport{0}, expectedDiscordants{0}, - encounteredM{true}, toRemove{true}, inverted{saAnnoIn.isInverted()}, - fuzzy{saAnnoIn.isDistant()}, strictFuzzy{saAnnoIn.isDistant()}, - strictFuzzyCandidate{true}, distant{saAnnoIn.isDistant()}, - suspicious{saAnnoIn.isSuspicious()}, - semiSuspicious{saAnnoIn.isSemiSuspicious()}, - properPairErrorProne{saAnnoIn.isProperPairErrorProne()}, - supportingIndices{} {} + SuppAlignmentAnno::SuppAlignmentAnno(int emittingBpChrIndex, int emittingBpPos, + const SuppAlignmentAnno &saAnnoIn) + : chrIndex{emittingBpChrIndex}, + pos{saAnnoIn.isDistant() + ? max(1, static_cast( + round(emittingBpPos - 1.5 * DEFAULTREADLENGTH))) + : emittingBpPos}, + extendedPos{ + saAnnoIn.isDistant() + ? static_cast(emittingBpPos + 1.5 * DEFAULTREADLENGTH) + : emittingBpPos}, + support{0}, secondarySupport{0}, mateSupport{0}, expectedDiscordants{0}, + encounteredM{true}, toRemove{true}, inverted{saAnnoIn.isInverted()}, + fuzzy{saAnnoIn.isDistant()}, strictFuzzy{saAnnoIn.isDistant()}, + strictFuzzyCandidate{true}, distant{saAnnoIn.isDistant()}, + suspicious{saAnnoIn.isSuspicious()}, + semiSuspicious{saAnnoIn.isSemiSuspicious()}, + properPairErrorProne{saAnnoIn.isProperPairErrorProne()}, + supportingIndices{} {} -string -SuppAlignmentAnno::print() const { - string outStr; - outStr.reserve(36); - string invStr{}; - if (inverted) { - invStr.append("_INV"); - } - if (encounteredM) { - outStr.append("|"); - } else { - invStr.append("|"); - } - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - if (!fuzzy || pos == extendedPos) { - outStr.append(chrConverter.indexToChr[chrIndex]) - .append(":") - .append(strtk::type_to_string(pos)); - } else { - outStr.append(chrConverter.indexToChr[chrIndex]) - .append(":") - .append(strtk::type_to_string(pos)) - .append("-") - .append(strtk::type_to_string(extendedPos)); - } - outStr.append(invStr) - .append("(") - .append(strtk::type_to_string(support)) - .append(",") - .append(strtk::type_to_string(secondarySupport)) - .append(","); - if (!suspicious) { - outStr.append(strtk::type_to_string(mateSupport)); - if (semiSuspicious) { - outStr.append("?"); + string + SuppAlignmentAnno::print() const { + string outStr; + outStr.reserve(36); + string invStr{}; + if (inverted) { + invStr.append("_INV"); } - } else { - outStr.append("!"); - } - outStr.append("/") - .append(strtk::type_to_string(expectedDiscordants)) - .append(")"); - if (properPairErrorProne) { - outStr.append("#"); + if (encounteredM) { + outStr.append("|"); + } else { + invStr.append("|"); + } + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + if (!fuzzy || pos == extendedPos) { + outStr.append(chrConverter.indexToChr[chrIndex]) + .append(":") + .append(strtk::type_to_string(pos)); + } else { + outStr.append(chrConverter.indexToChr[chrIndex]) + .append(":") + .append(strtk::type_to_string(pos)) + .append("-") + .append(strtk::type_to_string(extendedPos)); + } + outStr.append(invStr) + .append("(") + .append(strtk::type_to_string(support)) + .append(",") + .append(strtk::type_to_string(secondarySupport)) + .append(","); + if (!suspicious) { + outStr.append(strtk::type_to_string(mateSupport)); + if (semiSuspicious) { + outStr.append("?"); + } + } else { + outStr.append("!"); + } + outStr.append("/") + .append(strtk::type_to_string(expectedDiscordants)) + .append(")"); + if (properPairErrorProne) { + outStr.append("#"); + } + return outStr; } - return outStr; -} -bool -SuppAlignmentAnno::saCloseness(const SuppAlignmentAnno &rhs, - int fuzziness) const { - // inverted == rhs.isInverted() && //test - if (chrIndex == rhs.getChrIndex()) { - if (strictFuzzy || rhs.isStrictFuzzy()) { - if (pos <= rhs.getExtendedPos() && rhs.getPos() <= extendedPos) { - return true; - } - fuzziness = 2.5 * DEFAULTREADLENGTH; - if (rhs.getPos() >= extendedPos) { - return (rhs.getPos() - extendedPos) <= fuzziness; + bool + SuppAlignmentAnno::saCloseness(const SuppAlignmentAnno &rhs, + int fuzziness) const { + // inverted == rhs.isInverted() && //test + if (chrIndex == rhs.getChrIndex()) { + if (strictFuzzy || rhs.isStrictFuzzy()) { + if (pos <= rhs.getExtendedPos() && rhs.getPos() <= extendedPos) { + return true; + } + fuzziness = 2.5 * DEFAULTREADLENGTH; + if (rhs.getPos() >= extendedPos) { + return (rhs.getPos() - extendedPos) <= fuzziness; + } + return (pos - rhs.getExtendedPos()) <= fuzziness; + // return (rhs.getPos() - fuzziness) <= (extendedPos + + //fuzziness) && (pos - fuzziness) <= (rhs.getExtendedPos() + + //fuzziness); + } else { + return abs(pos - rhs.getPos()) <= fuzziness; } - return (pos - rhs.getExtendedPos()) <= fuzziness; - // return (rhs.getPos() - fuzziness) <= (extendedPos + - //fuzziness) && (pos - fuzziness) <= (rhs.getExtendedPos() + - //fuzziness); } else { - return abs(pos - rhs.getPos()) <= fuzziness; + return false; } - } else { - return false; } -} -bool -SuppAlignmentAnno::saClosenessDirectional(const SuppAlignmentAnno &rhs, - int fuzziness) const { - // inverted == rhs.isInverted() && //test - if (chrIndex == rhs.getChrIndex() && encounteredM == rhs.isEncounteredM()) { - if (strictFuzzy || rhs.isStrictFuzzy()) { - if (pos <= rhs.getExtendedPos() && rhs.getPos() <= extendedPos) { - return true; - } - fuzziness = 2.5 * DEFAULTREADLENGTH; - if (rhs.getPos() >= extendedPos) { - return (rhs.getPos() - extendedPos) <= fuzziness; + bool + SuppAlignmentAnno::saClosenessDirectional(const SuppAlignmentAnno &rhs, + int fuzziness) const { + // inverted == rhs.isInverted() && //test + if (chrIndex == rhs.getChrIndex() && encounteredM == rhs.isEncounteredM()) { + if (strictFuzzy || rhs.isStrictFuzzy()) { + if (pos <= rhs.getExtendedPos() && rhs.getPos() <= extendedPos) { + return true; + } + fuzziness = 2.5 * DEFAULTREADLENGTH; + if (rhs.getPos() >= extendedPos) { + return (rhs.getPos() - extendedPos) <= fuzziness; + } + return (pos - rhs.getExtendedPos()) <= fuzziness; + } else { + return abs(pos - rhs.getPos()) <= fuzziness; } - return (pos - rhs.getExtendedPos()) <= fuzziness; } else { - return abs(pos - rhs.getPos()) <= fuzziness; + return false; } - } else { - return false; } -} -void -SuppAlignmentAnno::mergeMrefSa(const SuppAlignmentAnno &mrefSa) { - support = max(support, mrefSa.getSupport()); - secondarySupport = max(secondarySupport, mrefSa.getSecondarySupport()); - for (auto index : mrefSa.getSupportingIndices()) { - supportingIndices.push_back(index); - } - sort(supportingIndices.begin(), supportingIndices.end()); - supportingIndices.erase( - unique(supportingIndices.begin(), supportingIndices.end()), - supportingIndices.end()); - if (mrefSa.getExpectedDiscordants() > 0 && expectedDiscordants > 0) { - if ((0.0 + mrefSa.getMateSupport()) / mrefSa.getExpectedDiscordants() > - (0.0 + mateSupport) / expectedDiscordants) { + void + SuppAlignmentAnno::mergeMrefSa(const SuppAlignmentAnno &mrefSa) { + support = max(support, mrefSa.getSupport()); + secondarySupport = max(secondarySupport, mrefSa.getSecondarySupport()); + for (auto index : mrefSa.getSupportingIndices()) { + supportingIndices.push_back(index); + } + sort(supportingIndices.begin(), supportingIndices.end()); + supportingIndices.erase( + unique(supportingIndices.begin(), supportingIndices.end()), + supportingIndices.end()); + if (mrefSa.getExpectedDiscordants() > 0 && expectedDiscordants > 0) { + if ((0.0 + mrefSa.getMateSupport()) / mrefSa.getExpectedDiscordants() > + (0.0 + mateSupport) / expectedDiscordants) { + mateSupport = mrefSa.getMateSupport(); + expectedDiscordants = mrefSa.getExpectedDiscordants(); + } + } else if (mrefSa.getExpectedDiscordants() > 0) { mateSupport = mrefSa.getMateSupport(); expectedDiscordants = mrefSa.getExpectedDiscordants(); } - } else if (mrefSa.getExpectedDiscordants() > 0) { - mateSupport = mrefSa.getMateSupport(); - expectedDiscordants = mrefSa.getExpectedDiscordants(); - } - if (!mrefSa.isSemiSuspicious() && semiSuspicious) { - semiSuspicious = false; + if (!mrefSa.isSemiSuspicious() && semiSuspicious) { + semiSuspicious = false; + } } -} -void -SuppAlignmentAnno::finalizeSupportingIndices() { - sort(supportingIndices.begin(), supportingIndices.end()); - supportingIndices.erase( - unique(supportingIndices.begin(), supportingIndices.end()), - supportingIndices.end()); - support = static_cast(supportingIndices.size()); - secondarySupport = 0; -} + void + SuppAlignmentAnno::finalizeSupportingIndices() { + sort(supportingIndices.begin(), supportingIndices.end()); + supportingIndices.erase( + unique(supportingIndices.begin(), supportingIndices.end()), + supportingIndices.end()); + support = static_cast(supportingIndices.size()); + secondarySupport = 0; + } } /* namespace sophia */ diff --git a/src/SvEvent.cpp b/src/SvEvent.cpp index 1e7e3b7..acc062c 100644 --- a/src/SvEvent.cpp +++ b/src/SvEvent.cpp @@ -29,1804 +29,1816 @@ namespace sophia { -using namespace std; + int SvEvent::GERMLINEOFFSETTHRESHOLD{}; + double SvEvent::RELAXEDBPFREQTHRESHOLD{}; + double SvEvent::BPFREQTHRESHOLD{}; + double SvEvent::ARTIFACTFREQLOWTHRESHOLD{}; + double SvEvent::ARTIFACTFREQHIGHTHRESHOLD{}; + double SvEvent::CLONALITYLOWTHRESHOLD{}; + double SvEvent::CLONALITYSTRICTLOWTHRESHOLD{}; + double SvEvent::CLONALITYHIGHTHRESHOLD{}; + int SvEvent::HALFDEFAULTREADLENGTH{}; + int SvEvent::GERMLINEDBLIMIT{}; + string SvEvent::PIDSINMREFSTR{}; + boost::format SvEvent::doubleFormatter{"%.3f"}; + bool SvEvent::ABRIDGEDOUTPUT{true}; + bool SvEvent::NOCONTROLMODE{false}; + bool SvEvent::DEBUGMODE{false}; + const vector SvEvent::EVENTTYPES{"UNKNOWN", "DEL", "DUP", + "TRA", "INV", "CONTAMINATION"}; -int SvEvent::GERMLINEOFFSETTHRESHOLD{}; -double SvEvent::RELAXEDBPFREQTHRESHOLD{}; -double SvEvent::BPFREQTHRESHOLD{}; -double SvEvent::ARTIFACTFREQLOWTHRESHOLD{}; -double SvEvent::ARTIFACTFREQHIGHTHRESHOLD{}; -double SvEvent::CLONALITYLOWTHRESHOLD{}; -double SvEvent::CLONALITYSTRICTLOWTHRESHOLD{}; -double SvEvent::CLONALITYHIGHTHRESHOLD{}; -int SvEvent::HALFDEFAULTREADLENGTH{}; -int SvEvent::GERMLINEDBLIMIT{}; -string SvEvent::PIDSINMREFSTR{}; -boost::format SvEvent::doubleFormatter{"%.3f"}; -bool SvEvent::ABRIDGEDOUTPUT{true}; -bool SvEvent::NOCONTROLMODE{false}; -bool SvEvent::DEBUGMODE{false}; -const vector SvEvent::EVENTTYPES{"UNKNOWN", "DEL", "DUP", - "TRA", "INV", "CONTAMINATION"}; - -SvEvent::SvEvent(const BreakpointReduced &bp1In, const BreakpointReduced &bp2In, - const SuppAlignmentAnno &sa1In, const SuppAlignmentAnno &sa2In, - const vector> &overhangDb) - : toRemove{false}, contaminationCandidate{0}, - chrIndex1{bp1In.getChrIndex()}, pos1{bp1In.getPos()}, - chrIndex2{bp2In.getChrIndex()}, pos2{bp2In.getPos()}, - lineIndex1{bp1In.getLineIndex()}, - lineIndex2{bp2In.getLineIndex()}, eventType{0}, eventSize{0}, - inverted{sa1In.isInverted() || sa2In.isInverted()}, distant{false}, - overhang1Compensation{false}, overhang2Compensation{false}, - overhang1Index{-1}, overhang2Index{-1}, overhang1lengthRatio{0}, - overhang2lengthRatio{0}, inputScore{2}, eventScore{0}, - totalEvidence1{sa1In.getSupport() + sa1In.getSecondarySupport() + - sa1In.getMateSupport()}, - span1{bp1In.getNormalSpans()}, - totalEvidence2{sa2In.getSupport() + sa2In.getSecondarySupport() + - sa2In.getMateSupport()}, - span2{bp2In.getNormalSpans()}, evidenceLevel1{0}, - evidenceLevel2{0}, mrefHits1{bp1In.getMrefHits().getNumConsevativeHits()}, - mrefHits1Conservative{true}, - mrefHits2{bp2In.getMrefHits().getNumConsevativeHits()}, - mrefHits2Conservative{true}, germline{false}, - germlineClonality1{bp1In.getGermlineInfo().getConservativeClonality()}, - germlineStatus1{bp1In.getGermlineInfo().getConservativeClonality() > - 0.15}, - germlineClonality2{bp2In.getGermlineInfo().getConservativeClonality()}, - germlineStatus2{bp2In.getGermlineInfo().getConservativeClonality() > - 0.15}, - selectedSa1{sa1In}, selectedSa2{sa2In}, - mateRatio1{sa1In.getExpectedDiscordants() > 0 - ? sa1In.getMateSupport() / - (0.0 + sa1In.getExpectedDiscordants()) - : 1.0}, - mateRatio2{sa2In.getExpectedDiscordants() > 0 - ? sa2In.getMateSupport() / - (0.0 + sa2In.getExpectedDiscordants()) - : 1.0}, - suspicious{0}, semiSuspicious{sa1In.isSemiSuspicious() || - sa2In.isSemiSuspicious()} { - // auto messageMode = selectedSa1.getChrIndex() == 11 && - //(selectedSa1.getPos() == 2261373 && selectedSa2.getPos() == 2148480); - auto posDifferential = pos1 - pos2; - determineEventTypeAndSize(posDifferential, selectedSa2.isEncounteredM()); - if (chrIndex1 != chrIndex2) { - distant = true; - } else if (selectedSa1.getExpectedDiscordants() > 0 || - selectedSa2.getExpectedDiscordants() > 0) { - distant = true; - } else if (eventSize > 1500) { - distant = true; - } - if (distant && chrIndex1 == chrIndex2 && - (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate() || - selectedSa2.isFuzzy() || selectedSa2.isStrictFuzzyCandidate())) { - if (eventSize < 5000) { - distant = false; + SvEvent::SvEvent(const BreakpointReduced &bp1In, const BreakpointReduced &bp2In, + const SuppAlignmentAnno &sa1In, const SuppAlignmentAnno &sa2In, + const vector> &overhangDb) + : toRemove{false}, contaminationCandidate{0}, + chrIndex1{bp1In.getChrIndex()}, pos1{bp1In.getPos()}, + chrIndex2{bp2In.getChrIndex()}, pos2{bp2In.getPos()}, + lineIndex1{bp1In.getLineIndex()}, + lineIndex2{bp2In.getLineIndex()}, eventType{0}, eventSize{0}, + inverted{sa1In.isInverted() || sa2In.isInverted()}, distant{false}, + overhang1Compensation{false}, overhang2Compensation{false}, + overhang1Index{-1}, overhang2Index{-1}, overhang1lengthRatio{0}, + overhang2lengthRatio{0}, inputScore{2}, eventScore{0}, + totalEvidence1{sa1In.getSupport() + sa1In.getSecondarySupport() + + sa1In.getMateSupport()}, + span1{bp1In.getNormalSpans()}, + totalEvidence2{sa2In.getSupport() + sa2In.getSecondarySupport() + + sa2In.getMateSupport()}, + span2{bp2In.getNormalSpans()}, evidenceLevel1{0}, + evidenceLevel2{0}, mrefHits1{bp1In.getMrefHits().getNumConsevativeHits()}, + mrefHits1Conservative{true}, + mrefHits2{bp2In.getMrefHits().getNumConsevativeHits()}, + mrefHits2Conservative{true}, germline{false}, + germlineClonality1{bp1In.getGermlineInfo().getConservativeClonality()}, + germlineStatus1{bp1In.getGermlineInfo().getConservativeClonality() > + 0.15}, + germlineClonality2{bp2In.getGermlineInfo().getConservativeClonality()}, + germlineStatus2{bp2In.getGermlineInfo().getConservativeClonality() > + 0.15}, + selectedSa1{sa1In}, selectedSa2{sa2In}, + mateRatio1{sa1In.getExpectedDiscordants() > 0 + ? sa1In.getMateSupport() / + (0.0 + sa1In.getExpectedDiscordants()) + : 1.0}, + mateRatio2{sa2In.getExpectedDiscordants() > 0 + ? sa2In.getMateSupport() / + (0.0 + sa2In.getExpectedDiscordants()) + : 1.0}, + suspicious{0}, semiSuspicious{sa1In.isSemiSuspicious() || + sa2In.isSemiSuspicious()} { + // auto messageMode = selectedSa1.getChrIndex() == 11 && + //(selectedSa1.getPos() == 2261373 && selectedSa2.getPos() == 2148480); + auto posDifferential = pos1 - pos2; + determineEventTypeAndSize(posDifferential, selectedSa2.isEncounteredM()); + if (chrIndex1 != chrIndex2) { + distant = true; + } else if (selectedSa1.getExpectedDiscordants() > 0 || + selectedSa2.getExpectedDiscordants() > 0) { + distant = true; + } else if (eventSize > 1500) { + distant = true; + } + if (distant && chrIndex1 == chrIndex2 && + (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate() || + selectedSa2.isFuzzy() || selectedSa2.isStrictFuzzyCandidate())) { + if (eventSize < 5000) { + distant = false; + } } - } - auto clonalityRes1 = assessSvClonality( - bp1In, selectedSa1.getSupport() + selectedSa1.getSecondarySupport() + - selectedSa1.getMateSupport()); - artifactRatio1 = clonalityRes1.first; - clonalityRatio1 = clonalityRes1.second; - clonalityStatus1 = - assessBreakpointClonalityStatus(clonalityRatio1, bp1In, bp2In); - auto clonalityRes2 = assessSvClonality( - bp2In, selectedSa2.getSupport() + selectedSa2.getSecondarySupport() + - selectedSa2.getMateSupport()); - artifactRatio2 = clonalityRes2.first; - clonalityRatio2 = clonalityRes2.second; - clonalityStatus2 = - assessBreakpointClonalityStatus(clonalityRatio2, bp1In, bp2In); + auto clonalityRes1 = assessSvClonality( + bp1In, selectedSa1.getSupport() + selectedSa1.getSecondarySupport() + + selectedSa1.getMateSupport()); + artifactRatio1 = clonalityRes1.first; + clonalityRatio1 = clonalityRes1.second; + clonalityStatus1 = + assessBreakpointClonalityStatus(clonalityRatio1, bp1In, bp2In); + auto clonalityRes2 = assessSvClonality( + bp2In, selectedSa2.getSupport() + selectedSa2.getSecondarySupport() + + selectedSa2.getMateSupport()); + artifactRatio2 = clonalityRes2.first; + clonalityRatio2 = clonalityRes2.second; + clonalityStatus2 = + assessBreakpointClonalityStatus(clonalityRatio2, bp1In, bp2In); - auto res1 = assessOverhangQualityCompensation(lineIndex1, overhangDb); - overhang1Index = res1.second; - overhang1Compensation = (clonalityStatus1 != EXTREME_SUBCLONAL) && - selectedSa1.isDistant() && res1.first; - auto res2 = assessOverhangQualityCompensation(lineIndex2, overhangDb); - overhang2Index = res2.second; - overhang2Compensation = (clonalityStatus2 != EXTREME_SUBCLONAL) && - selectedSa2.isDistant() && res2.first; + auto res1 = assessOverhangQualityCompensation(lineIndex1, overhangDb); + overhang1Index = res1.second; + overhang1Compensation = (clonalityStatus1 != EXTREME_SUBCLONAL) && + selectedSa1.isDistant() && res1.first; + auto res2 = assessOverhangQualityCompensation(lineIndex2, overhangDb); + overhang2Index = res2.second; + overhang2Compensation = (clonalityStatus2 != EXTREME_SUBCLONAL) && + selectedSa2.isDistant() && res2.first; - auto doubleSemiSuspicious = - (selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious()); - germlineClonality1 = - determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); - germlineStatus1 = germlineClonality1 > 0.15; - germlineClonality2 = - determineGermlineClonalityBp(bp2In, selectedSa2, germlineClonality2); - germlineStatus2 = germlineClonality2 > 0.15; + auto doubleSemiSuspicious = + (selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious()); + germlineClonality1 = + determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); + germlineStatus1 = germlineClonality1 > 0.15; + germlineClonality2 = + determineGermlineClonalityBp(bp2In, selectedSa2, germlineClonality2); + germlineStatus2 = germlineClonality2 > 0.15; - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && - !selectedSa2.isProperPairErrorProne() && - chrConverter.indexConverter[chrIndex1] < 23 && - chrConverter.indexConverter[chrIndex2] < 23; - auto splitSupportThreshold1 = - (strictNonDecoy && !selectedSa1.isSemiSuspicious() && - (mateRatio1 >= 0.6)) - ? 0 - : 2; - auto splitSupportThreshold2 = - (strictNonDecoy && !selectedSa2.isSemiSuspicious() && - (mateRatio2 >= 0.6)) - ? 0 - : 2; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && + !selectedSa2.isProperPairErrorProne() && + chrConverter.indexConverter[chrIndex1] < 23 && + chrConverter.indexConverter[chrIndex2] < 23; + auto splitSupportThreshold1 = + (strictNonDecoy && !selectedSa1.isSemiSuspicious() && + (mateRatio1 >= 0.6)) + ? 0 + : 2; + auto splitSupportThreshold2 = + (strictNonDecoy && !selectedSa2.isSemiSuspicious() && + (mateRatio2 >= 0.6)) + ? 0 + : 2; - if (selectedSa1.getSupport() > splitSupportThreshold1) { - ++evidenceLevel1; - } else { - if (strictNonDecoy && selectedSa1.getSupport() > 0 && - selectedSa1.getSecondarySupport() > splitSupportThreshold1) { + if (selectedSa1.getSupport() > splitSupportThreshold1) { ++evidenceLevel1; + } else { + if (strictNonDecoy && selectedSa1.getSupport() > 0 && + selectedSa1.getSecondarySupport() > splitSupportThreshold1) { + ++evidenceLevel1; + } } - } - if (selectedSa1.getSecondarySupport() > splitSupportThreshold1) { - ++evidenceLevel1; - } else { - if (strictNonDecoy && selectedSa1.getSecondarySupport() > 0 && - selectedSa1.getSupport() > splitSupportThreshold1) { + if (selectedSa1.getSecondarySupport() > splitSupportThreshold1) { ++evidenceLevel1; + } else { + if (strictNonDecoy && selectedSa1.getSecondarySupport() > 0 && + selectedSa1.getSupport() > splitSupportThreshold1) { + ++evidenceLevel1; + } } - } - if (selectedSa1.isDistant()) { - auto mateQuality = mateQualityConditions(selectedSa1); - if (selectedSa1.getMateSupport() > mateQuality.first && - mateRatio1 >= mateQuality.second) { - ++evidenceLevel1; - if (evidenceLevel1 < 3 && - (overhang1Compensation || - (!semiSuspicious && overhang2Compensation)) && - !doubleSemiSuspicious && strictNonDecoy) { - if ((selectedSa1.getMateSupport() > 2) || - (selectedSa1.getMateSupport() < 3 && - selectedSa1.getExpectedDiscordants() == - selectedSa1.getMateSupport())) { - ++evidenceLevel1; + if (selectedSa1.isDistant()) { + auto mateQuality = mateQualityConditions(selectedSa1); + if (selectedSa1.getMateSupport() > mateQuality.first && + mateRatio1 >= mateQuality.second) { + ++evidenceLevel1; + if (evidenceLevel1 < 3 && + (overhang1Compensation || + (!semiSuspicious && overhang2Compensation)) && + !doubleSemiSuspicious && strictNonDecoy) { + if ((selectedSa1.getMateSupport() > 2) || + (selectedSa1.getMateSupport() < 3 && + selectedSa1.getExpectedDiscordants() == + selectedSa1.getMateSupport())) { + ++evidenceLevel1; + } } } } - } - if (selectedSa2.getSupport() > splitSupportThreshold2) { - ++evidenceLevel2; - } else { - if (strictNonDecoy && selectedSa2.getSupport() > 0 && - selectedSa2.getSecondarySupport() > splitSupportThreshold2) { + if (selectedSa2.getSupport() > splitSupportThreshold2) { ++evidenceLevel2; + } else { + if (strictNonDecoy && selectedSa2.getSupport() > 0 && + selectedSa2.getSecondarySupport() > splitSupportThreshold2) { + ++evidenceLevel2; + } } - } - if (selectedSa2.getSecondarySupport() > splitSupportThreshold2) { - ++evidenceLevel2; - } else { - if (strictNonDecoy && selectedSa2.getSecondarySupport() > 0 && - selectedSa2.getSupport() > splitSupportThreshold2) { + if (selectedSa2.getSecondarySupport() > splitSupportThreshold2) { ++evidenceLevel2; + } else { + if (strictNonDecoy && selectedSa2.getSecondarySupport() > 0 && + selectedSa2.getSupport() > splitSupportThreshold2) { + ++evidenceLevel2; + } } - } - if (selectedSa2.isDistant()) { - auto mateQuality = mateQualityConditions(selectedSa2); - if (selectedSa2.getMateSupport() > mateQuality.first && - mateRatio2 >= mateQuality.second) { - ++evidenceLevel2; - if (evidenceLevel2 < 3 && - ((!semiSuspicious && overhang1Compensation) || - overhang2Compensation) && - !doubleSemiSuspicious && strictNonDecoy) { - if ((selectedSa2.getMateSupport() > 2) || - (selectedSa2.getMateSupport() < 3 && - selectedSa2.getExpectedDiscordants() == - selectedSa2.getMateSupport())) { - ++evidenceLevel2; + if (selectedSa2.isDistant()) { + auto mateQuality = mateQualityConditions(selectedSa2); + if (selectedSa2.getMateSupport() > mateQuality.first && + mateRatio2 >= mateQuality.second) { + ++evidenceLevel2; + if (evidenceLevel2 < 3 && + ((!semiSuspicious && overhang1Compensation) || + overhang2Compensation) && + !doubleSemiSuspicious && strictNonDecoy) { + if ((selectedSa2.getMateSupport() > 2) || + (selectedSa2.getMateSupport() < 3 && + selectedSa2.getExpectedDiscordants() == + selectedSa2.getMateSupport())) { + ++evidenceLevel2; + } } } } - } - auto mrefHits1Tmp = - processMrefHits(bp1In.getMrefHits(), selectedSa1, evidenceLevel1); - mrefHits1 = mrefHits1Tmp.second; - mrefHits1Conservative = mrefHits1Tmp.first; - auto mrefHits2Tmp = - processMrefHits(bp2In.getMrefHits(), selectedSa2, evidenceLevel2); - mrefHits2 = mrefHits2Tmp.second; - mrefHits2Conservative = mrefHits2Tmp.first; - if (!germlineStatus1 && germlineClonality1 > 0 && - mrefHits1 > GERMLINEDBLIMIT) { - germlineStatus1 = true; - } - if (!germlineStatus2 && germlineClonality2 > 0 && - mrefHits2 > GERMLINEDBLIMIT) { - germlineStatus2 = true; - } - germline = - (germlineStatus1 || germlineStatus2) && - !((selectedSa1.getSupport() + selectedSa1.getSecondarySupport()) > - 200 && - (selectedSa1.getSupport() + selectedSa2.getSecondarySupport()) > 200); + auto mrefHits1Tmp = + processMrefHits(bp1In.getMrefHits(), selectedSa1, evidenceLevel1); + mrefHits1 = mrefHits1Tmp.second; + mrefHits1Conservative = mrefHits1Tmp.first; + auto mrefHits2Tmp = + processMrefHits(bp2In.getMrefHits(), selectedSa2, evidenceLevel2); + mrefHits2 = mrefHits2Tmp.second; + mrefHits2Conservative = mrefHits2Tmp.first; + if (!germlineStatus1 && germlineClonality1 > 0 && + mrefHits1 > GERMLINEDBLIMIT) { + germlineStatus1 = true; + } + if (!germlineStatus2 && germlineClonality2 > 0 && + mrefHits2 > GERMLINEDBLIMIT) { + germlineStatus2 = true; + } + germline = + (germlineStatus1 || germlineStatus2) && + !((selectedSa1.getSupport() + selectedSa1.getSecondarySupport()) > + 200 && + (selectedSa1.getSupport() + selectedSa2.getSecondarySupport()) > 200); - assessSvArtifactStatus(bp1In, bp2In); - if (!selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious()) { - // auto messageMode = selectedSa1.getChrIndex() == 21 && - //selectedSa1.getPos() == 48119076; - auto score1 = filterMatch(bp1In, bp2In); - auto score2 = filterMatchUnknown(bp1In); - // if (messageMode) cerr << score1 << " " << score2 << - //"\n"; - if (score2 == 0 && score1 != 0) { - suspicious = score2; - semiSuspicious = false; + assessSvArtifactStatus(bp1In, bp2In); + if (!selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious()) { + // auto messageMode = selectedSa1.getChrIndex() == 21 && + //selectedSa1.getPos() == 48119076; + auto score1 = filterMatch(bp1In, bp2In); + auto score2 = filterMatchUnknown(bp1In); + // if (messageMode) cerr << score1 << " " << score2 << + //"\n"; + if (score2 == 0 && score1 != 0) { + suspicious = score2; + semiSuspicious = false; + } else { + suspicious = score1; + } } else { - suspicious = score1; + suspicious = filterMatch(bp1In, bp2In); + } + eventScore = assessEventScore(false, inputScore); + if (suspicious == 0 && eventScore > 2) { + assessContamination(overhangDb); } - } else { - suspicious = filterMatch(bp1In, bp2In); - } - eventScore = assessEventScore(false, inputScore); - if (suspicious == 0 && eventScore > 2) { - assessContamination(overhangDb); } -} -SvEvent::SvEvent(const BreakpointReduced &bp1In, const BreakpointReduced &bp2In, - const SuppAlignmentAnno &sa1In, - const vector> &overhangDb, - const SuppAlignmentAnno &dummySaIn) - : toRemove{false}, contaminationCandidate{0}, - chrIndex1{bp1In.getChrIndex()}, pos1{bp1In.getPos()}, - chrIndex2{bp2In.getChrIndex()}, pos2{bp2In.getPos()}, - lineIndex1{bp1In.getLineIndex()}, lineIndex2{bp2In.getLineIndex()}, - eventType{0}, eventSize{0}, inverted{sa1In.isInverted()}, distant{false}, - overhang1Compensation{false}, overhang2Compensation{false}, - overhang1Index{-1}, overhang2Index{-1}, overhang1lengthRatio{0}, - overhang2lengthRatio{0}, inputScore{1}, eventScore{0}, - totalEvidence1{sa1In.getSupport() + sa1In.getSecondarySupport() + - sa1In.getMateSupport()}, - span1{bp1In.getNormalSpans()}, - totalEvidence2{bp2In.getPairedBreaksSoft() + bp2In.getPairedBreaksHard() + - bp2In.getUnpairedBreaksSoft() + - bp2In.getUnpairedBreaksHard() + - bp2In.getBreaksShortIndel() + bp2In.getMateSupport()}, - span2{bp2In.getNormalSpans()}, evidenceLevel1{0}, - evidenceLevel2{0}, mrefHits1{bp1In.getMrefHits().getNumConsevativeHits()}, - mrefHits1Conservative{true}, - mrefHits2{bp2In.getMrefHits().getNumConsevativeHits()}, - mrefHits2Conservative{true}, germline{false}, - germlineClonality1{bp1In.getGermlineInfo().getConservativeClonality()}, - germlineStatus1{bp1In.getGermlineInfo().getConservativeClonality() > - 0.15}, - germlineClonality2{bp2In.getGermlineInfo().getConservativeClonality()}, - germlineStatus2{bp2In.getGermlineInfo().getConservativeClonality() > - 0.15}, - selectedSa1{sa1In}, selectedSa2{dummySaIn}, - mateRatio1{sa1In.getExpectedDiscordants() > 0 - ? sa1In.getMateSupport() / - (0.0 + sa1In.getExpectedDiscordants()) - : 1.0}, - mateRatio2{1.0}, suspicious{0}, semiSuspicious{sa1In.isSemiSuspicious()} { + SvEvent::SvEvent(const BreakpointReduced &bp1In, const BreakpointReduced &bp2In, + const SuppAlignmentAnno &sa1In, + const vector> &overhangDb, + const SuppAlignmentAnno &dummySaIn) + : toRemove{false}, + contaminationCandidate{0}, + chrIndex1{bp1In.getChrIndex()}, + pos1{bp1In.getPos()}, + chrIndex2{bp2In.getChrIndex()}, + pos2{bp2In.getPos()}, + lineIndex1{bp1In.getLineIndex()}, + lineIndex2{bp2In.getLineIndex()}, + eventType{0}, + eventSize{0}, + inverted{sa1In.isInverted()}, + distant{false}, + overhang1Compensation{false}, + overhang2Compensation{false}, + overhang1Index{-1}, + overhang2Index{-1}, + overhang1lengthRatio{0}, + overhang2lengthRatio{0}, + inputScore{1}, + eventScore{0}, + totalEvidence1{sa1In.getSupport() + sa1In.getSecondarySupport() + sa1In.getMateSupport()}, + span1{bp1In.getNormalSpans()}, + totalEvidence2{bp2In.getPairedBreaksSoft() + bp2In.getPairedBreaksHard() + + bp2In.getUnpairedBreaksSoft() + + bp2In.getUnpairedBreaksHard() + + bp2In.getBreaksShortIndel() + bp2In.getMateSupport()}, + span2{bp2In.getNormalSpans()}, + evidenceLevel1{0}, + evidenceLevel2{0}, + mrefHits1{bp1In.getMrefHits().getNumConsevativeHits()}, + mrefHits1Conservative{true}, + mrefHits2{bp2In.getMrefHits().getNumConsevativeHits()}, + mrefHits2Conservative{true}, + germline{false}, + germlineClonality1{bp1In.getGermlineInfo().getConservativeClonality()}, + germlineStatus1{bp1In.getGermlineInfo().getConservativeClonality() > 0.15}, + germlineClonality2{bp2In.getGermlineInfo().getConservativeClonality()}, + germlineStatus2{bp2In.getGermlineInfo().getConservativeClonality() > 0.15}, + selectedSa1{sa1In}, selectedSa2{dummySaIn}, + mateRatio1{sa1In.getExpectedDiscordants() > 0 + ? sa1In.getMateSupport() / + (0.0 + sa1In.getExpectedDiscordants()) + : 1.0}, + mateRatio2{1.0}, + suspicious{0}, + semiSuspicious{sa1In.isSemiSuspicious()} { - auto posDifferential = pos1 - pos2; - determineEventTypeAndSize( - posDifferential, (bp2In.getLeftCoverage() > bp2In.getRightCoverage())); - if (chrIndex1 != chrIndex2) { - distant = true; - } else if (selectedSa1.getExpectedDiscordants() > 0) { - distant = true; - } else if (eventSize > 1500) { - distant = true; - } - if (distant && chrIndex1 == chrIndex2 && - (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate())) { - if (eventSize < 5000) { + auto posDifferential = pos1 - pos2; + determineEventTypeAndSize( + posDifferential, (bp2In.getLeftCoverage() > bp2In.getRightCoverage())); + if (chrIndex1 != chrIndex2) { + distant = true; + } else if (selectedSa1.getExpectedDiscordants() > 0) { + distant = true; + } else if (eventSize > 1500) { + distant = true; + } + if (distant && chrIndex1 == chrIndex2 && + (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate())) { + if (eventSize < 5000) { + distant = false; + } + } + if (distant && (eventSize > 0) && (eventSize < 1000)) { distant = false; } - } - if (distant && (eventSize > 0) && (eventSize < 1000)) { - distant = false; - } - auto res1 = assessOverhangQualityCompensation(lineIndex1, overhangDb); - overhang1Index = res1.second; - auto res2 = assessOverhangQualityCompensation(lineIndex2, overhangDb); - overhang2Index = res2.second; + auto res1 = assessOverhangQualityCompensation(lineIndex1, overhangDb); + overhang1Index = res1.second; + auto res2 = assessOverhangQualityCompensation(lineIndex2, overhangDb); + overhang2Index = res2.second; - auto mateEvidence1 = false; - auto mateEvidence2 = false; - germlineClonality1 = - determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); - germlineStatus1 = germlineClonality1 > 0.15; + auto mateEvidence1 = false; + auto mateEvidence2 = false; + germlineClonality1 = + determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); + germlineStatus1 = germlineClonality1 > 0.15; - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && - chrConverter.indexConverter[chrIndex1] < 23 && - chrConverter.indexConverter[chrIndex2] < 23; - auto splitSupportThreshold = - (strictNonDecoy && !selectedSa2.isSemiSuspicious() && - (mateRatio1 >= 0.66)) - ? 0 - : 2; + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && + chrConverter.indexConverter[chrIndex1] < 23 && + chrConverter.indexConverter[chrIndex2] < 23; + auto splitSupportThreshold = + (strictNonDecoy && !selectedSa2.isSemiSuspicious() && + (mateRatio1 >= 0.66)) + ? 0 + : 2; - if (selectedSa1.getSupport() > splitSupportThreshold) { - ++evidenceLevel1; - } else { - if (strictNonDecoy && selectedSa1.getSupport() > 0 && - selectedSa1.getSecondarySupport() > splitSupportThreshold) { + if (selectedSa1.getSupport() > splitSupportThreshold) { ++evidenceLevel1; + } else { + if (strictNonDecoy && selectedSa1.getSupport() > 0 && + selectedSa1.getSecondarySupport() > splitSupportThreshold) { + ++evidenceLevel1; + } } - } - if (selectedSa1.getSecondarySupport() > splitSupportThreshold) { - ++evidenceLevel1; - } else { - if (strictNonDecoy && selectedSa1.getSecondarySupport() > 0 && - selectedSa1.getSupport() > splitSupportThreshold) { + if (selectedSa1.getSecondarySupport() > splitSupportThreshold) { ++evidenceLevel1; - } - } - if (selectedSa1.isDistant()) { - if (!selectedSa1.isStrictFuzzyCandidate() || - (selectedSa1.isStrictFuzzyCandidate() && - selectedSa1.getMateSupport() > 4)) { - auto mateQualityCriteria = selectedSa1.isProperPairErrorProne() || - selectedSa1.isSemiSuspicious() || - selectedSa1.isStrictFuzzyCandidate(); - if (mateRatio1 >= 0.4 && - (!mateQualityCriteria || - (mateQualityCriteria && selectedSa1.getMateSupport() > 4))) { + } else { + if (strictNonDecoy && selectedSa1.getSecondarySupport() > 0 && + selectedSa1.getSupport() > splitSupportThreshold) { ++evidenceLevel1; - mateEvidence1 = true; } } - } + if (selectedSa1.isDistant()) { + if (!selectedSa1.isStrictFuzzyCandidate() || + (selectedSa1.isStrictFuzzyCandidate() && + selectedSa1.getMateSupport() > 4)) { + auto mateQualityCriteria = selectedSa1.isProperPairErrorProne() || + selectedSa1.isSemiSuspicious() || + selectedSa1.isStrictFuzzyCandidate(); + if (mateRatio1 >= 0.4 && + (!mateQualityCriteria || + (mateQualityCriteria && selectedSa1.getMateSupport() > 4))) { + ++evidenceLevel1; + mateEvidence1 = true; + } + } + } - if (bp2In.getPairedBreaksSoft() + bp2In.getUnpairedBreaksSoft() > 0) { - ++evidenceLevel2; - } - if (bp2In.getPairedBreaksHard() + bp2In.getUnpairedBreaksHard() > 0) { - ++evidenceLevel2; - } - if (selectedSa1.isDistant()) { - if (bp2In.getMateSupport() > 4) { + if (bp2In.getPairedBreaksSoft() + bp2In.getUnpairedBreaksSoft() > 0) { ++evidenceLevel2; - mateEvidence2 = true; } - } - auto mrefHits1Tmp = - processMrefHits(bp1In.getMrefHits(), selectedSa1, evidenceLevel1); - mrefHits1 = mrefHits1Tmp.second; - mrefHits1Conservative = mrefHits1Tmp.first; - auto mrefHits2Tmp = - processMrefHits(bp2In.getMrefHits(), selectedSa2, evidenceLevel2); - mrefHits2 = mrefHits2Tmp.second; - mrefHits2Conservative = mrefHits2Tmp.first; - if (!germlineStatus1 && germlineClonality1 > 0 && - mrefHits1 > GERMLINEDBLIMIT) { - germlineStatus1 = true; - } - if (!germlineStatus2 && germlineClonality2 > 0 && - mrefHits2 > GERMLINEDBLIMIT) { - germlineStatus2 = true; - } - germline = (germlineStatus1 || germlineStatus2) && // - !((selectedSa1.getSupport() + - selectedSa1.getSecondarySupport()) > 200 && - (bp2In.getPairedBreaksSoft() + bp2In.getPairedBreaksHard() + - bp2In.getUnpairedBreaksSoft() + - bp2In.getUnpairedBreaksHard()) > 200); - - if (!distant) { - if (mateEvidence1) { - --evidenceLevel1; - totalEvidence1 -= selectedSa1.getMateSupport(); + if (bp2In.getPairedBreaksHard() + bp2In.getUnpairedBreaksHard() > 0) { + ++evidenceLevel2; } - if (mateEvidence2) { - --evidenceLevel2; - totalEvidence2 -= bp2In.getMateSupport(); + if (selectedSa1.isDistant()) { + if (bp2In.getMateSupport() > 4) { + ++evidenceLevel2; + mateEvidence2 = true; + } } - } - auto clonalityRes1 = assessSvClonality( - bp1In, selectedSa1.getSupport() + selectedSa1.getSecondarySupport() + - selectedSa1.getMateSupport()); - artifactRatio1 = clonalityRes1.first; - clonalityRatio1 = clonalityRes1.second; - clonalityStatus1 = - assessBreakpointClonalityStatusSingle(clonalityRatio1, bp1In, bp2In); - auto clonalityRes2 = assessSvClonality( - bp2In, bp2In.getPairedBreaksSoft() + bp2In.getPairedBreaksHard() + - bp2In.getUnpairedBreaksSoft()); - artifactRatio2 = clonalityRes2.first; - clonalityRatio2 = clonalityRes2.second; - clonalityStatus2 = - assessBreakpointClonalityStatusSingle(clonalityRatio2, bp1In, bp2In); + auto mrefHits1Tmp = + processMrefHits(bp1In.getMrefHits(), selectedSa1, evidenceLevel1); + mrefHits1 = mrefHits1Tmp.second; + mrefHits1Conservative = mrefHits1Tmp.first; + auto mrefHits2Tmp = + processMrefHits(bp2In.getMrefHits(), selectedSa2, evidenceLevel2); + mrefHits2 = mrefHits2Tmp.second; + mrefHits2Conservative = mrefHits2Tmp.first; + if (!germlineStatus1 && germlineClonality1 > 0 && + mrefHits1 > GERMLINEDBLIMIT) { + germlineStatus1 = true; + } + if (!germlineStatus2 && germlineClonality2 > 0 && + mrefHits2 > GERMLINEDBLIMIT) { + germlineStatus2 = true; + } + germline = (germlineStatus1 || germlineStatus2) && // + !((selectedSa1.getSupport() + + selectedSa1.getSecondarySupport()) > 200 && + (bp2In.getPairedBreaksSoft() + bp2In.getPairedBreaksHard() + + bp2In.getUnpairedBreaksSoft() + + bp2In.getUnpairedBreaksHard()) > 200); - assessSvArtifactStatus(bp1In, bp2In); - suspicious = filterMatchSingle(bp1In, bp2In); - auto hardClipSuspiciousCall = - selectedSa1.getSupport() == 0 && - selectedSa1.getSecondarySupport() > 0 && - (selectedSa1.getMateSupport() <= selectedSa1.getSecondarySupport() + 4); - eventScore = assessEventScore(hardClipSuspiciousCall, inputScore); - if (suspicious == 0 && eventScore > 2) { - assessContamination(overhangDb); - } -} + if (!distant) { + if (mateEvidence1) { + --evidenceLevel1; + totalEvidence1 -= selectedSa1.getMateSupport(); + } + if (mateEvidence2) { + --evidenceLevel2; + totalEvidence2 -= bp2In.getMateSupport(); + } + } + auto clonalityRes1 = assessSvClonality( + bp1In, selectedSa1.getSupport() + selectedSa1.getSecondarySupport() + + selectedSa1.getMateSupport()); + artifactRatio1 = clonalityRes1.first; + clonalityRatio1 = clonalityRes1.second; + clonalityStatus1 = + assessBreakpointClonalityStatusSingle(clonalityRatio1, bp1In, bp2In); + auto clonalityRes2 = assessSvClonality( + bp2In, bp2In.getPairedBreaksSoft() + bp2In.getPairedBreaksHard() + + bp2In.getUnpairedBreaksSoft()); + artifactRatio2 = clonalityRes2.first; + clonalityRatio2 = clonalityRes2.second; + clonalityStatus2 = + assessBreakpointClonalityStatusSingle(clonalityRatio2, bp1In, bp2In); -SvEvent::SvEvent(const BreakpointReduced &bp1In, const SuppAlignmentAnno &sa1In, - GermlineMatch germlineInfo2, MrefMatch hitsInMref2In, - const vector> &overhangDb, - const SuppAlignmentAnno &dummySaIn) - : toRemove{false}, contaminationCandidate{0}, - chrIndex1{bp1In.getChrIndex()}, pos1{bp1In.getPos()}, - chrIndex2{sa1In.getChrIndex()}, pos2{sa1In.getPos()}, - lineIndex1{bp1In.getLineIndex()}, lineIndex2{-1}, eventType{0}, - eventSize{0}, inverted{sa1In.isInverted()}, distant{false}, - overhang1Compensation{false}, overhang2Compensation{false}, - overhang1Index{-1}, overhang2Index{-1}, overhang1lengthRatio{0}, - overhang2lengthRatio{0}, inputScore{0}, eventScore{0}, - totalEvidence1{sa1In.getSupport() + sa1In.getSecondarySupport() + - sa1In.getMateSupport()}, - span1{bp1In.getNormalSpans()}, totalEvidence2{0}, evidenceLevel1{0}, - evidenceLevel2{0}, mrefHits1{bp1In.getMrefHits().getNumConsevativeHits()}, - mrefHits1Conservative{true}, - mrefHits2{hitsInMref2In.getNumConsevativeHits()}, - mrefHits2Conservative{true}, germline{false}, - germlineClonality1{bp1In.getGermlineInfo().getConservativeClonality()}, - germlineStatus1{bp1In.getGermlineInfo().getConservativeClonality() > - 0.15}, - germlineClonality2{germlineInfo2.getClonality()}, - germlineStatus2{germlineInfo2.getClonality() > 0.15}, selectedSa1{sa1In}, - selectedSa2{dummySaIn}, - mateRatio1{sa1In.getExpectedDiscordants() > 0 - ? sa1In.getMateSupport() / - (0.0 + sa1In.getExpectedDiscordants()) - : 1.0}, - mateRatio2{1.0}, suspicious{0}, semiSuspicious{sa1In.isSemiSuspicious()} { - auto truePos2 = pos2; - if (chrIndex1 == chrIndex2) { - if (abs(pos1 - pos2) > abs(pos1 - sa1In.getExtendedPos())) { - truePos2 = sa1In.getExtendedPos(); + assessSvArtifactStatus(bp1In, bp2In); + suspicious = filterMatchSingle(bp1In, bp2In); + auto hardClipSuspiciousCall = + selectedSa1.getSupport() == 0 && + selectedSa1.getSecondarySupport() > 0 && + (selectedSa1.getMateSupport() <= selectedSa1.getSecondarySupport() + 4); + eventScore = assessEventScore(hardClipSuspiciousCall, inputScore); + if (suspicious == 0 && eventScore > 2) { + assessContamination(overhangDb); } } - auto posDifferential = pos1 - truePos2; - determineEventTypeAndSize(posDifferential, !selectedSa1.isEncounteredM()); - if (chrIndex1 != chrIndex2) { - distant = true; - } else if (selectedSa1.getExpectedDiscordants() > 0) { - distant = true; - } else if (eventSize > 2500) { - distant = true; - } - if (distant && selectedSa1.isFuzzy() && - bp1In.getChrIndex() == selectedSa1.getChrIndex() && - selectedSa1.isStrictFuzzyCandidate()) { - auto fuzDiff = selectedSa1.getExtendedPos() - selectedSa1.getPos(); - if (max(0, pos1 - fuzDiff) <= selectedSa1.getExtendedPos() && - selectedSa1.getPos() <= (pos1 + fuzDiff)) { - distant = false; - } else if (eventSize < 5000) { + + SvEvent::SvEvent(const BreakpointReduced &bp1In, const SuppAlignmentAnno &sa1In, + GermlineMatch germlineInfo2, MrefMatch hitsInMref2In, + const vector> &overhangDb, + const SuppAlignmentAnno &dummySaIn) + : toRemove{false}, contaminationCandidate{0}, + chrIndex1{bp1In.getChrIndex()}, pos1{bp1In.getPos()}, + chrIndex2{sa1In.getChrIndex()}, pos2{sa1In.getPos()}, + lineIndex1{bp1In.getLineIndex()}, lineIndex2{-1}, eventType{0}, + eventSize{0}, inverted{sa1In.isInverted()}, distant{false}, + overhang1Compensation{false}, overhang2Compensation{false}, + overhang1Index{-1}, overhang2Index{-1}, overhang1lengthRatio{0}, + overhang2lengthRatio{0}, inputScore{0}, eventScore{0}, + totalEvidence1{sa1In.getSupport() + sa1In.getSecondarySupport() + + sa1In.getMateSupport()}, + span1{bp1In.getNormalSpans()}, totalEvidence2{0}, evidenceLevel1{0}, + evidenceLevel2{0}, mrefHits1{bp1In.getMrefHits().getNumConsevativeHits()}, + mrefHits1Conservative{true}, + mrefHits2{hitsInMref2In.getNumConsevativeHits()}, + mrefHits2Conservative{true}, germline{false}, + germlineClonality1{bp1In.getGermlineInfo().getConservativeClonality()}, + germlineStatus1{bp1In.getGermlineInfo().getConservativeClonality() > + 0.15}, + germlineClonality2{germlineInfo2.getClonality()}, + germlineStatus2{germlineInfo2.getClonality() > 0.15}, selectedSa1{sa1In}, + selectedSa2{dummySaIn}, + mateRatio1{sa1In.getExpectedDiscordants() > 0 + ? sa1In.getMateSupport() / + (0.0 + sa1In.getExpectedDiscordants()) + : 1.0}, + mateRatio2{1.0}, suspicious{0}, semiSuspicious{sa1In.isSemiSuspicious()} { + auto truePos2 = pos2; + if (chrIndex1 == chrIndex2) { + if (abs(pos1 - pos2) > abs(pos1 - sa1In.getExtendedPos())) { + truePos2 = sa1In.getExtendedPos(); + } + } + auto posDifferential = pos1 - truePos2; + determineEventTypeAndSize(posDifferential, !selectedSa1.isEncounteredM()); + if (chrIndex1 != chrIndex2) { + distant = true; + } else if (selectedSa1.getExpectedDiscordants() > 0) { + distant = true; + } else if (eventSize > 2500) { + distant = true; + } + if (distant && selectedSa1.isFuzzy() && + bp1In.getChrIndex() == selectedSa1.getChrIndex() && + selectedSa1.isStrictFuzzyCandidate()) { + auto fuzDiff = selectedSa1.getExtendedPos() - selectedSa1.getPos(); + if (max(0, pos1 - fuzDiff) <= selectedSa1.getExtendedPos() && + selectedSa1.getPos() <= (pos1 + fuzDiff)) { + distant = false; + } else if (eventSize < 5000) { + distant = false; + } + } + if (distant && (eventSize > 0) && (eventSize < 2000)) { distant = false; } - } - if (distant && (eventSize > 0) && (eventSize < 2000)) { - distant = false; - } - if (chrIndex1 == chrIndex2 && !distant && eventType == 3) { - suspicious = 3; - return; - } - auto clonalityRes1 = assessSvClonality( - bp1In, selectedSa1.getSupport() + selectedSa1.getSecondarySupport() + - selectedSa1.getMateSupport()); - artifactRatio1 = clonalityRes1.first; - clonalityRatio1 = clonalityRes1.second; - clonalityStatus1 = - assessBreakpointClonalityStatusUnknown(clonalityRatio1, bp1In); + if (chrIndex1 == chrIndex2 && !distant && eventType == 3) { + suspicious = 3; + return; + } + auto clonalityRes1 = assessSvClonality( + bp1In, selectedSa1.getSupport() + selectedSa1.getSecondarySupport() + + selectedSa1.getMateSupport()); + artifactRatio1 = clonalityRes1.first; + clonalityRatio1 = clonalityRes1.second; + clonalityStatus1 = + assessBreakpointClonalityStatusUnknown(clonalityRatio1, bp1In); - auto res1 = assessOverhangQualityCompensation(lineIndex1, overhangDb); - overhang1Index = res1.second; - overhang1Compensation = (clonalityStatus1 != EXTREME_SUBCLONAL) && - selectedSa1.isDistant() && res1.first; + auto res1 = assessOverhangQualityCompensation(lineIndex1, overhangDb); + overhang1Index = res1.second; + overhang1Compensation = (clonalityStatus1 != EXTREME_SUBCLONAL) && + selectedSa1.isDistant() && res1.first; - auto mateEvidence1 = false; - auto additionalEvidence1 = false; - germlineClonality1 = - determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); - germlineStatus1 = germlineClonality1 > 0.15; + auto mateEvidence1 = false; + auto additionalEvidence1 = false; + germlineClonality1 = + determineGermlineClonalityBp(bp1In, selectedSa1, germlineClonality1); + germlineStatus1 = germlineClonality1 > 0.15; - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && - chrConverter.indexConverter[chrIndex1] < 23 && - chrConverter.indexConverter[chrIndex2] < 23; - auto splitSupportThreshold = - (strictNonDecoy && (mateRatio1 >= 0.66) ? 0 : 2); + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + auto strictNonDecoy = !selectedSa1.isProperPairErrorProne() && + chrConverter.indexConverter[chrIndex1] < 23 && + chrConverter.indexConverter[chrIndex2] < 23; + auto splitSupportThreshold = + (strictNonDecoy && (mateRatio1 >= 0.66) ? 0 : 2); - if (selectedSa1.getSupport() > splitSupportThreshold) { - ++evidenceLevel1; - } else { - if (strictNonDecoy && selectedSa1.getSupport() > 0 && - selectedSa1.getSecondarySupport() > splitSupportThreshold) { + if (selectedSa1.getSupport() > splitSupportThreshold) { ++evidenceLevel1; + } else { + if (strictNonDecoy && selectedSa1.getSupport() > 0 && + selectedSa1.getSecondarySupport() > splitSupportThreshold) { + ++evidenceLevel1; + } } - } - if (selectedSa1.getSecondarySupport() > splitSupportThreshold) { - ++evidenceLevel1; - } else { - if (strictNonDecoy && selectedSa1.getSecondarySupport() > 0 && - selectedSa1.getSupport() > splitSupportThreshold) { + if (selectedSa1.getSecondarySupport() > splitSupportThreshold) { ++evidenceLevel1; - } - } - if (selectedSa1.isDistant()) { - if (mateRatio1 >= 0.4) { - if (!(selectedSa1.isStrictFuzzyCandidate() || - selectedSa1.isProperPairErrorProne()) || - ((selectedSa1.isStrictFuzzyCandidate() || - selectedSa1.isProperPairErrorProne()) && - selectedSa1.getMateSupport() > 4)) { + } else { + if (strictNonDecoy && selectedSa1.getSecondarySupport() > 0 && + selectedSa1.getSupport() > splitSupportThreshold) { ++evidenceLevel1; - mateEvidence1 = true; - if (evidenceLevel1 < 3 && overhang1Compensation && - strictNonDecoy) { - if ((selectedSa1.getMateSupport() > 2) || - (selectedSa1.getMateSupport() < 3 && - selectedSa1.getExpectedDiscordants() == - selectedSa1.getMateSupport())) { - ++evidenceLevel1; - additionalEvidence1 = true; + } + } + if (selectedSa1.isDistant()) { + if (mateRatio1 >= 0.4) { + if (!(selectedSa1.isStrictFuzzyCandidate() || + selectedSa1.isProperPairErrorProne()) || + ((selectedSa1.isStrictFuzzyCandidate() || + selectedSa1.isProperPairErrorProne()) && + selectedSa1.getMateSupport() > 4)) { + ++evidenceLevel1; + mateEvidence1 = true; + if (evidenceLevel1 < 3 && overhang1Compensation && + strictNonDecoy) { + if ((selectedSa1.getMateSupport() > 2) || + (selectedSa1.getMateSupport() < 3 && + selectedSa1.getExpectedDiscordants() == + selectedSa1.getMateSupport())) { + ++evidenceLevel1; + additionalEvidence1 = true; + } } } } } - } - auto mrefHits1Tmp = - processMrefHits(bp1In.getMrefHits(), selectedSa1, evidenceLevel1); - mrefHits1 = mrefHits1Tmp.second; - mrefHits1Conservative = mrefHits1Tmp.first; - auto mrefHits2Tmp = processMrefHits(hitsInMref2In, selectedSa2, 0); - mrefHits2 = mrefHits2Tmp.second; - mrefHits2Conservative = mrefHits2Tmp.first; - if (!germlineStatus1 && germlineClonality1 > 0 && - mrefHits1 > GERMLINEDBLIMIT) { - germlineStatus1 = true; - } - if (!germlineStatus2 && germlineClonality2 > 0 && - mrefHits2 > GERMLINEDBLIMIT) { - germlineStatus2 = true; - } + auto mrefHits1Tmp = + processMrefHits(bp1In.getMrefHits(), selectedSa1, evidenceLevel1); + mrefHits1 = mrefHits1Tmp.second; + mrefHits1Conservative = mrefHits1Tmp.first; + auto mrefHits2Tmp = processMrefHits(hitsInMref2In, selectedSa2, 0); + mrefHits2 = mrefHits2Tmp.second; + mrefHits2Conservative = mrefHits2Tmp.first; + if (!germlineStatus1 && germlineClonality1 > 0 && + mrefHits1 > GERMLINEDBLIMIT) { + germlineStatus1 = true; + } + if (!germlineStatus2 && germlineClonality2 > 0 && + mrefHits2 > GERMLINEDBLIMIT) { + germlineStatus2 = true; + } - germline = - (germlineStatus1 || germlineStatus2) && - !((selectedSa1.getSupport() + selectedSa1.getSecondarySupport()) > 200); - if (!distant) { - if (mateEvidence1) { - --evidenceLevel1; - totalEvidence1 -= selectedSa1.getMateSupport(); + germline = + (germlineStatus1 || germlineStatus2) && + !((selectedSa1.getSupport() + selectedSa1.getSecondarySupport()) > 200); + if (!distant) { + if (mateEvidence1) { + --evidenceLevel1; + totalEvidence1 -= selectedSa1.getMateSupport(); + } + if (additionalEvidence1) { + --evidenceLevel1; + } } - if (additionalEvidence1) { - --evidenceLevel1; + assessSvArtifactStatusUnknown(); + suspicious = filterMatchUnknown(bp1In); + auto hardClipSuspiciousCall = + selectedSa1.getSupport() == 0 && + selectedSa1.getSecondarySupport() > 0 && + (selectedSa1.getMateSupport() <= selectedSa1.getSecondarySupport() + 4); + eventScore = assessEventScore(hardClipSuspiciousCall, inputScore); + if (suspicious == 0 && eventScore > 2) { + assessContamination(overhangDb); } } - assessSvArtifactStatusUnknown(); - suspicious = filterMatchUnknown(bp1In); - auto hardClipSuspiciousCall = - selectedSa1.getSupport() == 0 && - selectedSa1.getSecondarySupport() > 0 && - (selectedSa1.getMateSupport() <= selectedSa1.getSecondarySupport() + 4); - eventScore = assessEventScore(hardClipSuspiciousCall, inputScore); - if (suspicious == 0 && eventScore > 2) { - assessContamination(overhangDb); - } -} -void -SvEvent::determineEventTypeAndSize(int posDifferential, - bool matchEncounteredM) { - // static vector EVENTTYPES { "UNKNOWN", "DEL", "DUP", "TRA", - //"INV", "CONTAMINATION" }; - if (chrIndex1 != chrIndex2) { - eventType = 3; - eventSize = -1; - } else { - eventSize = abs(posDifferential); - if (posDifferential < 0) { - if (inverted) { - eventType = 4; - } else { - if (selectedSa1.isEncounteredM() && !matchEncounteredM) { - eventType = 1; - } else if (!selectedSa1.isEncounteredM() && matchEncounteredM) { - eventType = 2; + void + SvEvent::determineEventTypeAndSize(int posDifferential, + bool matchEncounteredM) { + // static vector EVENTTYPES { "UNKNOWN", "DEL", "DUP", "TRA", + //"INV", "CONTAMINATION" }; + if (chrIndex1 != chrIndex2) { + eventType = 3; + eventSize = -1; + } else { + eventSize = abs(posDifferential); + if (posDifferential < 0) { + if (inverted) { + eventType = 4; } else { - eventType = 3; + if (selectedSa1.isEncounteredM() && !matchEncounteredM) { + eventType = 1; + } else if (!selectedSa1.isEncounteredM() && matchEncounteredM) { + eventType = 2; + } else { + eventType = 3; + } } - } - } else if (posDifferential > 0) { - if (inverted) { - eventType = 4; - } else { - if (selectedSa1.isEncounteredM() && !matchEncounteredM) { - eventType = 2; - } else if (!selectedSa1.isEncounteredM() && matchEncounteredM) { - eventType = 1; + } else if (posDifferential > 0) { + if (inverted) { + eventType = 4; } else { - eventType = 3; + if (selectedSa1.isEncounteredM() && !matchEncounteredM) { + eventType = 2; + } else if (!selectedSa1.isEncounteredM() && matchEncounteredM) { + eventType = 1; + } else { + eventType = 3; + } } + } else { + eventType = 3; + suspicious = 3; } - } else { - eventType = 3; - suspicious = 3; } } -} - -pair -SvEvent::mateQualityConditions(const SuppAlignmentAnno &sa) { - // auto messageMode = sa.getChrIndex() == 11 && (sa.getPos() == 2261373 || - //sa.getPos() == 2148480); - auto doubleSemiSuspicious = - (selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious()) || - (selectedSa1.isSemiSuspicious() && - selectedSa2.isProperPairErrorProne()) || - (selectedSa1.isProperPairErrorProne() && - selectedSa2.isSemiSuspicious()); - auto mateLowQualityCriteriaTier1 = sa.isProperPairErrorProne() || - doubleSemiSuspicious || - (mateRatio1 + mateRatio2) < 1.1; - auto mateLowQualityCriteriaTier2 = - sa.isSemiSuspicious() && sa.isStrictFuzzyCandidate(); - auto mateLowQualityCriteriaTier3 = sa.isSemiSuspicious() || sa.isFuzzy(); - auto mateLowQualityCriteriaTier4 = sa.isStrictFuzzyCandidate(); - if (mateLowQualityCriteriaTier1 && mateLowQualityCriteriaTier2) { - return {9, 0.8}; - } else if (mateLowQualityCriteriaTier1 || inputScore == 0) { - return {4, 0.8}; - } else if (mateLowQualityCriteriaTier2) { - return {4, 0.6}; - } else if (mateLowQualityCriteriaTier3) { - return {2, 0.6}; - } else if (mateLowQualityCriteriaTier4) { - return {0, 0.6}; - } else if (sa.getMateSupport() < 10) { - return {0, 0.4}; - } else { - return {0, 0.33}; - } -} -pair -SvEvent::assessOverhangQualityCompensation( - int lineIndex, const vector> &overhangDb) const { - auto overhangIndex = -1; - auto compensation = false; - pair dummy{lineIndex, ""}; - auto lower = lower_bound(overhangDb.cbegin(), overhangDb.cend(), dummy); - auto it = overhangDb.cend(); - if (lower != overhangDb.cend()) { - if (lower->first == lineIndex) { - it = lower; - } else if (next(lower) != overhangDb.cend() && - next(lower)->first == lineIndex) { - it = next(lower); - } else if (lower != overhangDb.cbegin() && - prev(lower)->first == lineIndex) { - it = prev(lower); + pair + SvEvent::mateQualityConditions(const SuppAlignmentAnno &sa) { + // auto messageMode = sa.getChrIndex() == 11 && (sa.getPos() == 2261373 || + //sa.getPos() == 2148480); + auto doubleSemiSuspicious = + (selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious()) || + (selectedSa1.isSemiSuspicious() && + selectedSa2.isProperPairErrorProne()) || + (selectedSa1.isProperPairErrorProne() && + selectedSa2.isSemiSuspicious()); + auto mateLowQualityCriteriaTier1 = sa.isProperPairErrorProne() || + doubleSemiSuspicious || + (mateRatio1 + mateRatio2) < 1.1; + auto mateLowQualityCriteriaTier2 = + sa.isSemiSuspicious() && sa.isStrictFuzzyCandidate(); + auto mateLowQualityCriteriaTier3 = sa.isSemiSuspicious() || sa.isFuzzy(); + auto mateLowQualityCriteriaTier4 = sa.isStrictFuzzyCandidate(); + if (mateLowQualityCriteriaTier1 && mateLowQualityCriteriaTier2) { + return {9, 0.8}; + } else if (mateLowQualityCriteriaTier1 || inputScore == 0) { + return {4, 0.8}; + } else if (mateLowQualityCriteriaTier2) { + return {4, 0.6}; + } else if (mateLowQualityCriteriaTier3) { + return {2, 0.6}; + } else if (mateLowQualityCriteriaTier4) { + return {0, 0.6}; + } else if (sa.getMateSupport() < 10) { + return {0, 0.4}; + } else { + return {0, 0.33}; } } - if (it != overhangDb.cend()) { - auto overhangLength = 0; - auto maxOverhangLength = 0; - auto counts = 0; - overhangIndex = it - overhangDb.cbegin(); - for (const auto c : it->second) { - switch (c) { - case '(': - maxOverhangLength = max(maxOverhangLength, overhangLength); - overhangLength = 0; - break; - case ':': - overhangLength = 0; - ++counts; - break; - default: - ++overhangLength; - break; + + pair + SvEvent::assessOverhangQualityCompensation( + int lineIndex, const vector> &overhangDb) const { + auto overhangIndex = -1; + auto compensation = false; + pair dummy{lineIndex, ""}; + auto lower = lower_bound(overhangDb.cbegin(), overhangDb.cend(), dummy); + auto it = overhangDb.cend(); + if (lower != overhangDb.cend()) { + if (lower->first == lineIndex) { + it = lower; + } else if (next(lower) != overhangDb.cend() && + next(lower)->first == lineIndex) { + it = next(lower); + } else if (lower != overhangDb.cbegin() && + prev(lower)->first == lineIndex) { + it = prev(lower); } } - if (counts < 3) { - auto lengthRatio = (0.0 + maxOverhangLength) / - SuppAlignmentAnno::DEFAULTREADLENGTH; - compensation = 0.25 <= lengthRatio && lengthRatio <= 0.8; + if (it != overhangDb.cend()) { + auto overhangLength = 0; + auto maxOverhangLength = 0; + auto counts = 0; + overhangIndex = it - overhangDb.cbegin(); + for (const auto c : it->second) { + switch (c) { + case '(': + maxOverhangLength = max(maxOverhangLength, overhangLength); + overhangLength = 0; + break; + case ':': + overhangLength = 0; + ++counts; + break; + default: + ++overhangLength; + break; + } + } + if (counts < 3) { + auto lengthRatio = (0.0 + maxOverhangLength) / + SuppAlignmentAnno::DEFAULTREADLENGTH; + compensation = 0.25 <= lengthRatio && lengthRatio <= 0.8; + } } + return {compensation, overhangIndex}; } - return {compensation, overhangIndex}; -} -pair -SvEvent::processMrefHits(const MrefMatch &hitsInMref, - const SuppAlignmentAnno &sa, - int evidenceLevelIn) const { - auto initScore = hitsInMref.getNumConsevativeHits(); - auto distantHit = false; - auto saMatch = false; - short maxScore{0}; - short hits{initScore}; - for (const auto &saRef : hitsInMref.getSuppMatches()) { - if (saRef.saCloseness(sa, SuppAlignmentAnno::DEFAULTREADLENGTH * 6)) { - saMatch = true; - auto score = saRef.getSecondarySupport(); - if (score > maxScore) { - maxScore = score; - if (maxScore > initScore) { - hits = maxScore; - distantHit = true; + pair + SvEvent::processMrefHits(const MrefMatch &hitsInMref, + const SuppAlignmentAnno &sa, + int evidenceLevelIn) const { + auto initScore = hitsInMref.getNumConsevativeHits(); + auto distantHit = false; + auto saMatch = false; + short maxScore{0}; + short hits{initScore}; + for (const auto &saRef : hitsInMref.getSuppMatches()) { + if (saRef.saCloseness(sa, SuppAlignmentAnno::DEFAULTREADLENGTH * 6)) { + saMatch = true; + auto score = saRef.getSecondarySupport(); + if (score > maxScore) { + maxScore = score; + if (maxScore > initScore) { + hits = maxScore; + distantHit = true; + } } } } + if (!distantHit && + (selectedSa1.isStrictFuzzy() || selectedSa2.isStrictFuzzy()) && + evidenceLevelIn < 3) { + hits = hitsInMref.getNumHits(); + } + return {!saMatch, hits}; } - if (!distantHit && - (selectedSa1.isStrictFuzzy() || selectedSa2.isStrictFuzzy()) && - evidenceLevelIn < 3) { - hits = hitsInMref.getNumHits(); - } - return {!saMatch, hits}; -} -double -SvEvent::determineGermlineClonalityBp(const BreakpointReduced &bp1, - const SuppAlignmentAnno &sa, - double clonalityInit) const { - auto maxClonality = clonalityInit; - if (sa.isDistant()) { - auto i = 0; - for (const auto &saRef : bp1.getGermlineInfo().getSuppMatches()) { - if (saRef.saCloseness(sa, - SuppAlignmentAnno::DEFAULTREADLENGTH * 6)) { - auto clonality = bp1.getGermlineInfo().getClonalities()[i]; - if (clonality > maxClonality) { - maxClonality = clonality; + double + SvEvent::determineGermlineClonalityBp(const BreakpointReduced &bp1, + const SuppAlignmentAnno &sa, + double clonalityInit) const { + auto maxClonality = clonalityInit; + if (sa.isDistant()) { + auto i = 0; + for (const auto &saRef : bp1.getGermlineInfo().getSuppMatches()) { + if (saRef.saCloseness(sa, + SuppAlignmentAnno::DEFAULTREADLENGTH * 6)) { + auto clonality = bp1.getGermlineInfo().getClonalities()[i]; + if (clonality > maxClonality) { + maxClonality = clonality; + } + break; } - break; + ++i; } - ++i; } + return maxClonality; } - return maxClonality; -} -int -SvEvent::filterMatch(const BreakpointReduced &bp1, - const BreakpointReduced &bp2) { - if (suspicious != 0) { - return suspicious; - } - if (selectedSa1.isSuspicious() || selectedSa2.isSuspicious() || - (selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious() && - !(evidenceLevel1 == 3 && evidenceLevel2 == 3))) { - return 1; - } - if ((selectedSa1.getExpectedDiscordants() > 0 && mateRatio1 < 0.1) || - (selectedSa2.getExpectedDiscordants() > 0 && mateRatio2 < 0.1)) { - return 2; - } - if (mrefHits1 > GERMLINEDBLIMIT) { - if (selectedSa1.isSemiSuspicious() && evidenceLevel1 < 3) { - if (!(selectedSa1.getSupport() > 9 || - selectedSa1.getSecondarySupport() > 9 || - selectedSa1.getMateSupport() > 9 || overhang1Compensation)) { - return 4; - } + int + SvEvent::filterMatch(const BreakpointReduced &bp1, + const BreakpointReduced &bp2) { + if (suspicious != 0) { + return suspicious; } - } - if (mrefHits2 > GERMLINEDBLIMIT) { - if (selectedSa2.isSemiSuspicious() && evidenceLevel2 < 3) { - if (!(selectedSa2.getSupport() > 9 || - selectedSa2.getSecondarySupport() > 9 || - selectedSa1.getMateSupport() > 9 || overhang2Compensation)) { - return 5; - } + if (selectedSa1.isSuspicious() || selectedSa2.isSuspicious() || + (selectedSa1.isSemiSuspicious() && selectedSa2.isSemiSuspicious() && + !(evidenceLevel1 == 3 && evidenceLevel2 == 3))) { + return 1; } - } - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - if (clonalityRatio1 < CLONALITYSTRICTLOWTHRESHOLD && - clonalityRatio2 < CLONALITYSTRICTLOWTHRESHOLD) { - return 4; - } else if ((!germlineStatus1 || !germlineStatus2) && // - (mrefHits1 > GERMLINEDBLIMIT || - mrefHits2 > GERMLINEDBLIMIT) && - (eventSize > 0) && (eventSize < HALFDEFAULTREADLENGTH)) { - return 5; + if ((selectedSa1.getExpectedDiscordants() > 0 && mateRatio1 < 0.1) || + (selectedSa2.getExpectedDiscordants() > 0 && mateRatio2 < 0.1)) { + return 2; } - } - if (!distant) { - if (semiSuspicious) { - return 13; + if (mrefHits1 > GERMLINEDBLIMIT) { + if (selectedSa1.isSemiSuspicious() && evidenceLevel1 < 3) { + if (!(selectedSa1.getSupport() > 9 || + selectedSa1.getSecondarySupport() > 9 || + selectedSa1.getMateSupport() > 9 || overhang1Compensation)) { + return 4; + } + } } - if (selectedSa1.isStrictFuzzy() || selectedSa2.isStrictFuzzy()) { - return 13; + if (mrefHits2 > GERMLINEDBLIMIT) { + if (selectedSa2.isSemiSuspicious() && evidenceLevel2 < 3) { + if (!(selectedSa2.getSupport() > 9 || + selectedSa2.getSecondarySupport() > 9 || + selectedSa1.getMateSupport() > 9 || overhang2Compensation)) { + return 5; + } + } } - if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { - if (totalEvidence1 < 5 || totalEvidence2 < 5) { - return 13; + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + if (clonalityRatio1 < CLONALITYSTRICTLOWTHRESHOLD && + clonalityRatio2 < CLONALITYSTRICTLOWTHRESHOLD) { + return 4; + } else if ((!germlineStatus1 || !germlineStatus2) && // + (mrefHits1 > GERMLINEDBLIMIT || + mrefHits2 > GERMLINEDBLIMIT) && + (eventSize > 0) && (eventSize < HALFDEFAULTREADLENGTH)) { + return 5; } } - } else { - if (bp1.getChrIndex() != bp2.getChrIndex()) { - if (selectedSa1.getMateSupport() == 0) { - return 6; - } else if (selectedSa2.getMateSupport() == 0) { - return 7; - } - } - auto threshold = - (semiSuspicious || selectedSa1.isProperPairErrorProne() || - selectedSa2.isProperPairErrorProne()) - ? 5 - : 3; - if (selectedSa1.getSupport() < threshold && - selectedSa1.getSecondarySupport() < threshold && - selectedSa2.getMateSupport() < threshold && - selectedSa2.getSupport() < threshold && - selectedSa2.getSecondarySupport() < threshold && - selectedSa2.getMateSupport() < threshold) { - return 8; - } - if (mateRatio1 < 0.4 && mateRatio2 < 0.4) { - return 11; - } - if (mateRatio1 < 0.25 || mateRatio2 < 0.25) { - return 12; - } - if (selectedSa1.isFuzzy() || selectedSa2.isFuzzy()) { - if (bp1.getChrIndex() == bp2.getChrIndex()) { - if (selectedSa1.isStrictFuzzy() || - selectedSa2.isStrictFuzzy()) { - if (abs(bp1.getPos() - bp2.getPos()) < 5000) { - return 14; - } - } + if (!distant) { + if (semiSuspicious) { + return 13; + } + if (selectedSa1.isStrictFuzzy() || selectedSa2.isStrictFuzzy()) { + return 13; } if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { - return 173; + if (totalEvidence1 < 5 || totalEvidence2 < 5) { + return 13; + } } - if (selectedSa1.isFuzzy() && selectedSa2.isFuzzy()) { - if (mateRatio1 < 0.5 || mateRatio2 < 0.5) { - return 174; + } else { + if (bp1.getChrIndex() != bp2.getChrIndex()) { + if (selectedSa1.getMateSupport() == 0) { + return 6; + } else if (selectedSa2.getMateSupport() == 0) { + return 7; } } - if (selectedSa1.isFuzzy() && selectedSa1.getSupport() == 0 && - selectedSa1.getSecondarySupport() == 0 && - selectedSa2.isSemiSuspicious()) { - return 175; + auto threshold = + (semiSuspicious || selectedSa1.isProperPairErrorProne() || + selectedSa2.isProperPairErrorProne()) + ? 5 + : 3; + if (selectedSa1.getSupport() < threshold && + selectedSa1.getSecondarySupport() < threshold && + selectedSa2.getMateSupport() < threshold && + selectedSa2.getSupport() < threshold && + selectedSa2.getSecondarySupport() < threshold && + selectedSa2.getMateSupport() < threshold) { + return 8; } - if (selectedSa2.isFuzzy() && selectedSa2.getSupport() == 0 && - selectedSa2.getSecondarySupport() == 0 && - selectedSa1.isSemiSuspicious()) { - return 176; + if (mateRatio1 < 0.4 && mateRatio2 < 0.4) { + return 11; } - } - } - if (bp1.getChrIndex() != bp2.getChrIndex() || - abs(bp1.getPos() - bp2.getPos()) > 150) { - auto eventTotal1 = bp1.getPairedBreaksSoft() + - bp1.getPairedBreaksHard() + - bp1.getUnpairedBreaksSoft(); - auto eventTotal2 = bp2.getPairedBreaksSoft() + - bp2.getPairedBreaksHard() + - bp2.getUnpairedBreaksSoft(); - if (bp1.getBreaksShortIndel() > eventTotal1 || - bp2.getBreaksShortIndel() > eventTotal2) { - return 16; - } - } - if (clonalityStatus1 == EXTREME_SUBCLONAL) { - return 39; - } - if (clonalityStatus2 == EXTREME_SUBCLONAL) { - return 40; - } - if (artifactStatus == ARTIFACT) { - return 41; - } - if (NOCONTROLMODE || germlineStatus1 || germlineStatus2) { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - return 42; - } - } - if (mrefHits1 > BPFREQTHRESHOLD) { - if (NOCONTROLMODE || germlineStatus1 || germlineStatus2 || - mrefHits2 > GERMLINEDBLIMIT) { - return 431; - } - if (mrefHits1 > RELAXEDBPFREQTHRESHOLD * 2.5) { - return 431; - } - if (!(mrefHits1Conservative && evidenceLevel1 == 3 && - evidenceLevel2 > 1 && mrefHits2 == 0 && !selectedSa1.isFuzzy() && - !selectedSa2.isFuzzy())) { - if (mrefHits1 > RELAXEDBPFREQTHRESHOLD) { - return 431; + if (mateRatio1 < 0.25 || mateRatio2 < 0.25) { + return 12; } - } - } - if (mrefHits2 > BPFREQTHRESHOLD) { - if (NOCONTROLMODE || germlineStatus1 || germlineStatus2 || - mrefHits1 > GERMLINEDBLIMIT) { - return 432; - } - if (mrefHits2 > RELAXEDBPFREQTHRESHOLD * 2.5) { - return 432; - } - if (!(mrefHits2Conservative && evidenceLevel1 > 1 && - evidenceLevel2 == 3 && mrefHits1 == 0 && !selectedSa1.isFuzzy() && - !selectedSa2.isFuzzy())) { - if (mrefHits2 > RELAXEDBPFREQTHRESHOLD) { - return 432; + if (selectedSa1.isFuzzy() || selectedSa2.isFuzzy()) { + if (bp1.getChrIndex() == bp2.getChrIndex()) { + if (selectedSa1.isStrictFuzzy() || + selectedSa2.isStrictFuzzy()) { + if (abs(bp1.getPos() - bp2.getPos()) < 5000) { + return 14; + } + } + } + if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { + return 173; + } + if (selectedSa1.isFuzzy() && selectedSa2.isFuzzy()) { + if (mateRatio1 < 0.5 || mateRatio2 < 0.5) { + return 174; + } + } + if (selectedSa1.isFuzzy() && selectedSa1.getSupport() == 0 && + selectedSa1.getSecondarySupport() == 0 && + selectedSa2.isSemiSuspicious()) { + return 175; + } + if (selectedSa2.isFuzzy() && selectedSa2.getSupport() == 0 && + selectedSa2.getSecondarySupport() == 0 && + selectedSa1.isSemiSuspicious()) { + return 176; + } } } - } - return suspicious; -} - -int -SvEvent::filterMatchSingle(const BreakpointReduced &bp1, - const BreakpointReduced &bp2) { - if (suspicious != 0) { - return suspicious; - } - if (selectedSa1.isSuspicious() || semiSuspicious) { - return 1; - } - if (selectedSa1.getExpectedDiscordants() > 0 && mateRatio1 < 0.1) { - return 2; - } - if (mrefHits1 > GERMLINEDBLIMIT && - !(bp1.getChrIndex() == 999 || bp1.getChrIndex() == 1000)) { - return 171; - } - if (mrefHits2 > GERMLINEDBLIMIT && - !(bp2.getChrIndex() == 999 || bp2.getChrIndex() == 1000)) { - return 171; - } - if (!distant) { - if (inverted) { - return 22; - } - if (eventSize > 0 && eventSize < HALFDEFAULTREADLENGTH) { - return 23; - } - if (eventType == 3) { - return 23; - } - if (eventSize > 150) { + if (bp1.getChrIndex() != bp2.getChrIndex() || + abs(bp1.getPos() - bp2.getPos()) > 150) { auto eventTotal1 = bp1.getPairedBreaksSoft() + bp1.getPairedBreaksHard() + bp1.getUnpairedBreaksSoft(); auto eventTotal2 = bp2.getPairedBreaksSoft() + bp2.getPairedBreaksHard() + bp2.getUnpairedBreaksSoft(); - if ((bp1.getBreaksShortIndel() / (0.0 + eventTotal1) > 0.5)) { - return 20; - } else if ((bp2.getBreaksShortIndel() / (0.0 + eventTotal2)) > - 0.5) { - return 21; + if (bp1.getBreaksShortIndel() > eventTotal1 || + bp2.getBreaksShortIndel() > eventTotal2) { + return 16; } } - if (selectedSa1.isFuzzy() || selectedSa1.isSemiSuspicious() || - evidenceLevel1 == 1 || - (selectedSa1.getSupport() + selectedSa1.getSecondarySupport()) < - 3) { - return 25; + if (clonalityStatus1 == EXTREME_SUBCLONAL) { + return 39; } - if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { - if (totalEvidence1 < 5) { - return 25; - } + if (clonalityStatus2 == EXTREME_SUBCLONAL) { + return 40; } - } else { - if (selectedSa1.getSupport() < 3 && - selectedSa1.getSecondarySupport() < 3 && - selectedSa1.getMateSupport() < 3) { - return 18; + if (artifactStatus == ARTIFACT) { + return 41; } - if (bp1.getChrIndex() != bp2.getChrIndex()) { - if (selectedSa1.getMateSupport() == 0) { - return 18; - } else if (evidenceLevel1 == 1 && bp2.getMateSupport() == 0) { - return 19; + if (NOCONTROLMODE || germlineStatus1 || germlineStatus2) { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + return 42; } } - if (selectedSa1.getMateSupport() < 3 || mateRatio1 < 0.4) { - return 24; - } - if (selectedSa1.isFuzzy() || (selectedSa1.getSupport() + - selectedSa1.getSecondarySupport()) < 3) { - if (bp1.getChrIndex() == bp2.getChrIndex()) { - if (abs(bp1.getPos() - bp2.getPos()) < 5000) { - return 26; - } - if (inverted && abs(bp1.getPos() - bp2.getPos()) < 10000) { - return 27; + if (mrefHits1 > BPFREQTHRESHOLD) { + if (NOCONTROLMODE || germlineStatus1 || germlineStatus2 || + mrefHits2 > GERMLINEDBLIMIT) { + return 431; + } + if (mrefHits1 > RELAXEDBPFREQTHRESHOLD * 2.5) { + return 431; + } + if (!(mrefHits1Conservative && evidenceLevel1 == 3 && + evidenceLevel2 > 1 && mrefHits2 == 0 && !selectedSa1.isFuzzy() && + !selectedSa2.isFuzzy())) { + if (mrefHits1 > RELAXEDBPFREQTHRESHOLD) { + return 431; } } - if (selectedSa1.isFuzzy() && selectedSa1.getSupport() == 0 && - selectedSa1.getSecondarySupport() == 0) { - return 271; + } + if (mrefHits2 > BPFREQTHRESHOLD) { + if (NOCONTROLMODE || germlineStatus1 || germlineStatus2 || + mrefHits1 > GERMLINEDBLIMIT) { + return 432; + } + if (mrefHits2 > RELAXEDBPFREQTHRESHOLD * 2.5) { + return 432; + } + if (!(mrefHits2Conservative && evidenceLevel1 > 1 && + evidenceLevel2 == 3 && mrefHits1 == 0 && !selectedSa1.isFuzzy() && + !selectedSa2.isFuzzy())) { + if (mrefHits2 > RELAXEDBPFREQTHRESHOLD) { + return 432; + } } } + return suspicious; } - auto eventTotal1 = bp1.getPairedBreaksSoft() + bp1.getPairedBreaksHard() + - bp1.getUnpairedBreaksSoft(); - auto eventTotal2 = bp2.getPairedBreaksSoft() + bp2.getPairedBreaksHard() + - bp2.getUnpairedBreaksSoft(); - if (bp1.getBreaksShortIndel() > eventTotal1 || - bp2.getBreaksShortIndel() > eventTotal2) { - return 28; - } - if (clonalityStatus1 == EXTREME_SUBCLONAL) { - return 39; - } - if (clonalityStatus2 == EXTREME_SUBCLONAL) { - return 40; - } - if (artifactStatus == ARTIFACT) { - return 41; - } - if (NOCONTROLMODE || germlineStatus1 || germlineStatus2) { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - return 42; + + int + SvEvent::filterMatchSingle(const BreakpointReduced &bp1, + const BreakpointReduced &bp2) { + if (suspicious != 0) { + return suspicious; } - } - if (mrefHits1 > BPFREQTHRESHOLD) { - if (chrIndex1 != 999 || chrIndex2 == 999 || - mrefHits2 > BPFREQTHRESHOLD) { - return 431; + if (selectedSa1.isSuspicious() || semiSuspicious) { + return 1; } - if (mrefHits1 > RELAXEDBPFREQTHRESHOLD || NOCONTROLMODE || - germlineStatus1 || germlineStatus2 || mrefHits2 > GERMLINEDBLIMIT) { - return 431; + if (selectedSa1.getExpectedDiscordants() > 0 && mateRatio1 < 0.1) { + return 2; } - } - if (mrefHits2 > BPFREQTHRESHOLD) { - if (chrIndex1 == 999 || chrIndex2 != 999 || - mrefHits1 > BPFREQTHRESHOLD) { - return 432; + if (mrefHits1 > GERMLINEDBLIMIT && + !(bp1.getChrIndex() == 999 || bp1.getChrIndex() == 1000)) { + return 171; } - if (mrefHits2 > RELAXEDBPFREQTHRESHOLD || NOCONTROLMODE || - germlineStatus1 || germlineStatus2 || mrefHits1 > GERMLINEDBLIMIT) { - return 432; + if (mrefHits2 > GERMLINEDBLIMIT && + !(bp2.getChrIndex() == 999 || bp2.getChrIndex() == 1000)) { + return 171; } - } - return suspicious; -} - -int -SvEvent::filterMatchUnknown(const BreakpointReduced &bp1) { - if (suspicious != 0) { - return suspicious; - } - if (selectedSa1.isSuspicious() || selectedSa1.isSemiSuspicious()) { - return 1; - } - if (selectedSa1.getExpectedDiscordants() > 0 && mateRatio1 < 0.1) { - return 2; - } - auto eventTotal1 = bp1.getPairedBreaksSoft() + bp1.getPairedBreaksHard() + - bp1.getUnpairedBreaksSoft(); - if (eventTotal1 + selectedSa1.getMateSupport() + bp1.getNormalSpans() < - 10) { - return 29; - } - if (mrefHits1 > GERMLINEDBLIMIT && - !(bp1.getChrIndex() == 999 || bp1.getChrIndex() == 1000)) { - return 301; - } - if (mrefHits2 > GERMLINEDBLIMIT && !(selectedSa1.getChrIndex() == 999 || - selectedSa1.getChrIndex() == 1000)) { - return 302; - } - if (!distant) { - if (inverted || totalEvidence1 < 5 || evidenceLevel1 == 1) { - return 30; - } - if (eventSize > 0 && eventSize < HALFDEFAULTREADLENGTH) { - return 31; + if (!distant) { + if (inverted) { + return 22; + } + if (eventSize > 0 && eventSize < HALFDEFAULTREADLENGTH) { + return 23; + } + if (eventType == 3) { + return 23; + } + if (eventSize > 150) { + auto eventTotal1 = bp1.getPairedBreaksSoft() + + bp1.getPairedBreaksHard() + + bp1.getUnpairedBreaksSoft(); + auto eventTotal2 = bp2.getPairedBreaksSoft() + + bp2.getPairedBreaksHard() + + bp2.getUnpairedBreaksSoft(); + if ((bp1.getBreaksShortIndel() / (0.0 + eventTotal1) > 0.5)) { + return 20; + } else if ((bp2.getBreaksShortIndel() / (0.0 + eventTotal2)) > + 0.5) { + return 21; + } + } + if (selectedSa1.isFuzzy() || selectedSa1.isSemiSuspicious() || + evidenceLevel1 == 1 || + (selectedSa1.getSupport() + selectedSa1.getSecondarySupport()) < + 3) { + return 25; + } + if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { + if (totalEvidence1 < 5) { + return 25; + } + } + } else { + if (selectedSa1.getSupport() < 3 && + selectedSa1.getSecondarySupport() < 3 && + selectedSa1.getMateSupport() < 3) { + return 18; + } + if (bp1.getChrIndex() != bp2.getChrIndex()) { + if (selectedSa1.getMateSupport() == 0) { + return 18; + } else if (evidenceLevel1 == 1 && bp2.getMateSupport() == 0) { + return 19; + } + } + if (selectedSa1.getMateSupport() < 3 || mateRatio1 < 0.4) { + return 24; + } + if (selectedSa1.isFuzzy() || (selectedSa1.getSupport() + + selectedSa1.getSecondarySupport()) < 3) { + if (bp1.getChrIndex() == bp2.getChrIndex()) { + if (abs(bp1.getPos() - bp2.getPos()) < 5000) { + return 26; + } + if (inverted && abs(bp1.getPos() - bp2.getPos()) < 10000) { + return 27; + } + } + if (selectedSa1.isFuzzy() && selectedSa1.getSupport() == 0 && + selectedSa1.getSecondarySupport() == 0) { + return 271; + } + } } - if (eventType == 3) { - return 31; + auto eventTotal1 = bp1.getPairedBreaksSoft() + bp1.getPairedBreaksHard() + + bp1.getUnpairedBreaksSoft(); + auto eventTotal2 = bp2.getPairedBreaksSoft() + bp2.getPairedBreaksHard() + + bp2.getUnpairedBreaksSoft(); + if (bp1.getBreaksShortIndel() > eventTotal1 || + bp2.getBreaksShortIndel() > eventTotal2) { + return 28; } - if (selectedSa1.isFuzzy() || selectedSa1.isSemiSuspicious() || - selectedSa1.isStrictFuzzyCandidate()) { - return 31; + if (clonalityStatus1 == EXTREME_SUBCLONAL) { + return 39; } - if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { - if (totalEvidence1 < 5) { - return 31; - } + if (clonalityStatus2 == EXTREME_SUBCLONAL) { + return 40; } - } else { - if (selectedSa1.getSupport() < 3 && - selectedSa1.getSecondarySupport() < 3 && - selectedSa1.getMateSupport() < 3) { - return 18; + if (artifactStatus == ARTIFACT) { + return 41; } - if (selectedSa1.getMateSupport() < 3) { - if ((mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) || - (evidenceLevel1 < 3 && selectedSa1.getSupport() < 5)) { - return 32; + if (NOCONTROLMODE || germlineStatus1 || germlineStatus2) { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + return 42; } } - if (mateRatio1 < 0.33 || - (evidenceLevel1 != 3 && totalEvidence1 < 5 && mateRatio1 < 0.5)) { - return 33; + if (mrefHits1 > BPFREQTHRESHOLD) { + if (chrIndex1 != 999 || chrIndex2 == 999 || + mrefHits2 > BPFREQTHRESHOLD) { + return 431; + } + if (mrefHits1 > RELAXEDBPFREQTHRESHOLD || NOCONTROLMODE || + germlineStatus1 || germlineStatus2 || mrefHits2 > GERMLINEDBLIMIT) { + return 431; + } } - if (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate()) { - if (selectedSa1.getSupport() == 0 && - selectedSa1.getSecondarySupport() == 0) { - return 36; + if (mrefHits2 > BPFREQTHRESHOLD) { + if (chrIndex1 == 999 || chrIndex2 != 999 || + mrefHits1 > BPFREQTHRESHOLD) { + return 432; + } + if (mrefHits2 > RELAXEDBPFREQTHRESHOLD || NOCONTROLMODE || + germlineStatus1 || germlineStatus2 || mrefHits1 > GERMLINEDBLIMIT) { + return 432; } } + return suspicious; } - if ((bp1.getBreaksShortIndel() > eventTotal1)) { - return 38; - } - if (clonalityStatus1 == EXTREME_SUBCLONAL) { - return 44; - } - if (artifactStatus == ARTIFACT) { - return 45; - } - if (NOCONTROLMODE || (germlineStatus1 || germlineStatus2)) { - if (NOCONTROLMODE) { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - return 46; + + int + SvEvent::filterMatchUnknown(const BreakpointReduced &bp1) { + if (suspicious != 0) { + return suspicious; + } + if (selectedSa1.isSuspicious() || selectedSa1.isSemiSuspicious()) { + return 1; + } + if (selectedSa1.getExpectedDiscordants() > 0 && mateRatio1 < 0.1) { + return 2; + } + auto eventTotal1 = bp1.getPairedBreaksSoft() + bp1.getPairedBreaksHard() + + bp1.getUnpairedBreaksSoft(); + if (eventTotal1 + selectedSa1.getMateSupport() + bp1.getNormalSpans() < + 10) { + return 29; + } + if (mrefHits1 > GERMLINEDBLIMIT && + !(bp1.getChrIndex() == 999 || bp1.getChrIndex() == 1000)) { + return 301; + } + if (mrefHits2 > GERMLINEDBLIMIT && !(selectedSa1.getChrIndex() == 999 || + selectedSa1.getChrIndex() == 1000)) { + return 302; + } + if (!distant) { + if (inverted || totalEvidence1 < 5 || evidenceLevel1 == 1) { + return 30; + } + if (eventSize > 0 && eventSize < HALFDEFAULTREADLENGTH) { + return 31; + } + if (eventType == 3) { + return 31; + } + if (selectedSa1.isFuzzy() || selectedSa1.isSemiSuspicious() || + selectedSa1.isStrictFuzzyCandidate()) { + return 31; } - } else { if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { - return 47; + if (totalEvidence1 < 5) { + return 31; + } + } + } else { + if (selectedSa1.getSupport() < 3 && + selectedSa1.getSecondarySupport() < 3 && + selectedSa1.getMateSupport() < 3) { + return 18; + } + if (selectedSa1.getMateSupport() < 3) { + if ((mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) || + (evidenceLevel1 < 3 && selectedSa1.getSupport() < 5)) { + return 32; + } + } + if (mateRatio1 < 0.33 || + (evidenceLevel1 != 3 && totalEvidence1 < 5 && mateRatio1 < 0.5)) { + return 33; + } + if (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate()) { + if (selectedSa1.getSupport() == 0 && + selectedSa1.getSecondarySupport() == 0) { + return 36; + } } } - } - if (mrefHits1 > BPFREQTHRESHOLD) { - if (chrIndex1 != 999 || selectedSa1.getChrIndex() == 999 || - mrefHits2 > BPFREQTHRESHOLD) { - return 471; + if ((bp1.getBreaksShortIndel() > eventTotal1)) { + return 38; } - if (mrefHits1 > 3 * BPFREQTHRESHOLD || NOCONTROLMODE || - germlineStatus1 || germlineStatus2 || mrefHits2 > GERMLINEDBLIMIT) { - return 471; + if (clonalityStatus1 == EXTREME_SUBCLONAL) { + return 44; } - } - if (mrefHits2 > BPFREQTHRESHOLD) { - if (chrIndex2 == 999 || selectedSa1.getChrIndex() != 999 || - mrefHits1 > BPFREQTHRESHOLD) { - return 472; + if (artifactStatus == ARTIFACT) { + return 45; } - if (mrefHits2 > 3 * BPFREQTHRESHOLD || NOCONTROLMODE || - germlineStatus1 || germlineStatus2 || mrefHits1 > GERMLINEDBLIMIT) { - return 472; + if (NOCONTROLMODE || (germlineStatus1 || germlineStatus2)) { + if (NOCONTROLMODE) { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + return 46; + } + } else { + if (mrefHits1 > GERMLINEDBLIMIT || mrefHits2 > GERMLINEDBLIMIT) { + return 47; + } + } + } + if (mrefHits1 > BPFREQTHRESHOLD) { + if (chrIndex1 != 999 || selectedSa1.getChrIndex() == 999 || + mrefHits2 > BPFREQTHRESHOLD) { + return 471; + } + if (mrefHits1 > 3 * BPFREQTHRESHOLD || NOCONTROLMODE || + germlineStatus1 || germlineStatus2 || mrefHits2 > GERMLINEDBLIMIT) { + return 471; + } } + if (mrefHits2 > BPFREQTHRESHOLD) { + if (chrIndex2 == 999 || selectedSa1.getChrIndex() != 999 || + mrefHits1 > BPFREQTHRESHOLD) { + return 472; + } + if (mrefHits2 > 3 * BPFREQTHRESHOLD || NOCONTROLMODE || + germlineStatus1 || germlineStatus2 || mrefHits1 > GERMLINEDBLIMIT) { + return 472; + } + } + return suspicious; } - return suspicious; -} -pair -SvEvent::assessSvClonality(const BreakpointReduced &bp, - int eventSupportTotal) const { - auto artifactTotal1 = bp.getLowQualSpansSoft() + bp.getLowQualBreaksSoft() + - bp.getRepetitiveOverhangBreaks(); - auto eventTotal1 = eventSupportTotal + bp.getUnpairedBreaksSoft(); - auto artifactRatio = - (artifactTotal1 + 0.0) / (artifactTotal1 + eventTotal1); - auto clonalityRatio = - (eventTotal1 + 0.0) / (eventTotal1 + bp.getNormalSpans()); - return {artifactRatio, clonalityRatio}; -} + pair + SvEvent::assessSvClonality(const BreakpointReduced &bp, + int eventSupportTotal) const { + auto artifactTotal1 = bp.getLowQualSpansSoft() + bp.getLowQualBreaksSoft() + + bp.getRepetitiveOverhangBreaks(); + auto eventTotal1 = eventSupportTotal + bp.getUnpairedBreaksSoft(); + auto artifactRatio = + (artifactTotal1 + 0.0) / (artifactTotal1 + eventTotal1); + auto clonalityRatio = + (eventTotal1 + 0.0) / (eventTotal1 + bp.getNormalSpans()); + return {artifactRatio, clonalityRatio}; + } -ClonalityStatus -SvEvent::assessBreakpointClonalityStatus(double clonalityRatioIn, - const BreakpointReduced &bp1, - const BreakpointReduced &bp2) const { - if (clonalityRatioIn < CLONALITYLOWTHRESHOLD) { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + ClonalityStatus + SvEvent::assessBreakpointClonalityStatus(double clonalityRatioIn, + const BreakpointReduced &bp1, + const BreakpointReduced &bp2) const { + if (clonalityRatioIn < CLONALITYLOWTHRESHOLD) { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + return EXTREME_SUBCLONAL; + } + if (distant) { + if ((selectedSa1.getMateSupport() > 9 && mateRatio1 >= 0.4) || + (selectedSa1.getMateSupport() > 4 && mateRatio1 >= 0.6)) { + return SUBCLONAL; + } + if ((selectedSa2.getMateSupport() > 9 && mateRatio2 >= 0.4) || + (selectedSa2.getMateSupport() > 4 && mateRatio2 >= 0.6)) { + return SUBCLONAL; + } + } return EXTREME_SUBCLONAL; + } else if (clonalityRatioIn >= CLONALITYHIGHTHRESHOLD) { + return HOMO; + } else { + return HETERO; } - if (distant) { - if ((selectedSa1.getMateSupport() > 9 && mateRatio1 >= 0.4) || - (selectedSa1.getMateSupport() > 4 && mateRatio1 >= 0.6)) { - return SUBCLONAL; + } + + ClonalityStatus + SvEvent::assessBreakpointClonalityStatusSingle( + double clonalityRatioIn, const BreakpointReduced &bp1, + const BreakpointReduced &bp2) const { + if (clonalityRatioIn < CLONALITYLOWTHRESHOLD) { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + return EXTREME_SUBCLONAL; } - if ((selectedSa2.getMateSupport() > 9 && mateRatio2 >= 0.4) || - (selectedSa2.getMateSupport() > 4 && mateRatio2 >= 0.6)) { + if (distant && + ((selectedSa1.getMateSupport() > 9 && mateRatio1 >= 0.5) || + (selectedSa1.getMateSupport() > 4 && mateRatio1 >= 0.8))) { return SUBCLONAL; + } else { + return EXTREME_SUBCLONAL; } + } else if (clonalityRatioIn >= CLONALITYHIGHTHRESHOLD) { + return HOMO; + } else { + return HETERO; } - return EXTREME_SUBCLONAL; - } else if (clonalityRatioIn >= CLONALITYHIGHTHRESHOLD) { - return HOMO; - } else { - return HETERO; } -} -ClonalityStatus -SvEvent::assessBreakpointClonalityStatusSingle( - double clonalityRatioIn, const BreakpointReduced &bp1, - const BreakpointReduced &bp2) const { - if (clonalityRatioIn < CLONALITYLOWTHRESHOLD) { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - return EXTREME_SUBCLONAL; - } - if (distant && - ((selectedSa1.getMateSupport() > 9 && mateRatio1 >= 0.5) || - (selectedSa1.getMateSupport() > 4 && mateRatio1 >= 0.8))) { - return SUBCLONAL; + ClonalityStatus + SvEvent::assessBreakpointClonalityStatusUnknown( + double clonalityRatioIn, const BreakpointReduced &bp1) const { + if (clonalityRatioIn < CLONALITYLOWTHRESHOLD) { + if (mrefHits1 > GERMLINEDBLIMIT) { + return EXTREME_SUBCLONAL; + } + if (distant && + ((selectedSa1.getMateSupport() > 9 && mateRatio1 >= 0.5) || + (selectedSa1.getMateSupport() > 4 && mateRatio1 >= 0.8))) { + return SUBCLONAL; + } else { + return EXTREME_SUBCLONAL; + } + } else if (clonalityRatioIn >= CLONALITYHIGHTHRESHOLD) { + return HOMO; } else { - return EXTREME_SUBCLONAL; + return HETERO; } - } else if (clonalityRatioIn >= CLONALITYHIGHTHRESHOLD) { - return HOMO; - } else { - return HETERO; } -} -ClonalityStatus -SvEvent::assessBreakpointClonalityStatusUnknown( - double clonalityRatioIn, const BreakpointReduced &bp1) const { - if (clonalityRatioIn < CLONALITYLOWTHRESHOLD) { - if (mrefHits1 > GERMLINEDBLIMIT) { - return EXTREME_SUBCLONAL; + void + SvEvent::assessSvArtifactStatus(const BreakpointReduced &bp1, + const BreakpointReduced &bp2) { + if (artifactRatio1 < ARTIFACTFREQLOWTHRESHOLD && + artifactRatio2 < ARTIFACTFREQLOWTHRESHOLD) { + artifactStatus = CLEAN; + return; } - if (distant && - ((selectedSa1.getMateSupport() > 9 && mateRatio1 >= 0.5) || - (selectedSa1.getMateSupport() > 4 && mateRatio1 >= 0.8))) { - return SUBCLONAL; - } else { - return EXTREME_SUBCLONAL; + if (artifactRatio1 > 0.85 && artifactRatio2 > 0.85) { + artifactStatus = ARTIFACT; + return; } - } else if (clonalityRatioIn >= CLONALITYHIGHTHRESHOLD) { - return HOMO; - } else { - return HETERO; - } -} - -void -SvEvent::assessSvArtifactStatus(const BreakpointReduced &bp1, - const BreakpointReduced &bp2) { - if (artifactRatio1 < ARTIFACTFREQLOWTHRESHOLD && - artifactRatio2 < ARTIFACTFREQLOWTHRESHOLD) { - artifactStatus = CLEAN; - return; - } - if (artifactRatio1 > 0.85 && artifactRatio2 > 0.85) { - artifactStatus = ARTIFACT; - return; - } - if (artifactRatio1 > 0.85 || artifactRatio2 > 0.85) { - if (!(evidenceLevel1 >= 2 || evidenceLevel2 >= 2)) { + if (artifactRatio1 > 0.85 || artifactRatio2 > 0.85) { + if (!(evidenceLevel1 >= 2 || evidenceLevel2 >= 2)) { + artifactStatus = ARTIFACT; + return; + } + } + if (artifactRatio1 > ARTIFACTFREQHIGHTHRESHOLD && + artifactRatio2 > ARTIFACTFREQHIGHTHRESHOLD && + (mrefHits1 > GERMLINEDBLIMIT || mrefHits1 > GERMLINEDBLIMIT)) { artifactStatus = ARTIFACT; return; } + if ((artifactRatio1 > ARTIFACTFREQHIGHTHRESHOLD || + artifactRatio2 > ARTIFACTFREQHIGHTHRESHOLD) && + (clonalityStatus1 == EXTREME_SUBCLONAL || + clonalityStatus2 == EXTREME_SUBCLONAL)) { + artifactStatus = ARTIFACT; + return; + } + artifactStatus = BORDERLINE; } - if (artifactRatio1 > ARTIFACTFREQHIGHTHRESHOLD && - artifactRatio2 > ARTIFACTFREQHIGHTHRESHOLD && - (mrefHits1 > GERMLINEDBLIMIT || mrefHits1 > GERMLINEDBLIMIT)) { - artifactStatus = ARTIFACT; - return; - } - if ((artifactRatio1 > ARTIFACTFREQHIGHTHRESHOLD || - artifactRatio2 > ARTIFACTFREQHIGHTHRESHOLD) && - (clonalityStatus1 == EXTREME_SUBCLONAL || - clonalityStatus2 == EXTREME_SUBCLONAL)) { - artifactStatus = ARTIFACT; - return; - } - artifactStatus = BORDERLINE; -} -void -SvEvent::assessSvArtifactStatusUnknown() { - if (artifactRatio1 < ARTIFACTFREQLOWTHRESHOLD && - artifactRatio2 < ARTIFACTFREQLOWTHRESHOLD) { - artifactStatus = CLEAN; - return; - } - if (artifactRatio1 > ARTIFACTFREQHIGHTHRESHOLD) { - artifactStatus = ARTIFACT; - return; + void + SvEvent::assessSvArtifactStatusUnknown() { + if (artifactRatio1 < ARTIFACTFREQLOWTHRESHOLD && + artifactRatio2 < ARTIFACTFREQLOWTHRESHOLD) { + artifactStatus = CLEAN; + return; + } + if (artifactRatio1 > ARTIFACTFREQHIGHTHRESHOLD) { + artifactStatus = ARTIFACT; + return; + } + artifactStatus = BORDERLINE; } - artifactStatus = BORDERLINE; -} -int -SvEvent::assessEventScore(bool hardClipSuspiciousCall, int inputScoreCategory) { - // inputscore 0: UNKNOWN partner - // inputscore 1: known partner with no SA signal - // inputscore 2: known partner with matching SA signal - if (inputScoreCategory == 0) { - if (distant) { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - if (selectedSa1.getSupport() > 30 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 10) { - eventType = 5; - return 1; - } - if (selectedSa1.getSupport() > 100 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 20) { - eventType = 5; - return 1; - } - } else if (mrefHits1 > GERMLINEDBLIMIT) { - if (selectedSa1.getSupport() > 50 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 10) { - eventType = 5; - return 1; + int + SvEvent::assessEventScore(bool hardClipSuspiciousCall, int inputScoreCategory) { + // inputscore 0: UNKNOWN partner + // inputscore 1: known partner with no SA signal + // inputscore 2: known partner with matching SA signal + if (inputScoreCategory == 0) { + if (distant) { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + if (selectedSa1.getSupport() > 30 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 10) { + eventType = 5; + return 1; + } + if (selectedSa1.getSupport() > 100 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 20) { + eventType = 5; + return 1; + } + } else if (mrefHits1 > GERMLINEDBLIMIT) { + if (selectedSa1.getSupport() > 50 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 10) { + eventType = 5; + return 1; + } + if (selectedSa1.getSupport() > 100 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 20) { + eventType = 5; + return 1; + } } - if (selectedSa1.getSupport() > 100 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 20) { - eventType = 5; + if ((selectedSa1.isFuzzy() || + selectedSa1.isStrictFuzzyCandidate())) { + if (evidenceLevel1 == 3 || + !selectedSa1.isStrictFuzzyCandidate()) { + if ((selectedSa1.getMateSupport() > 9 && + mateRatio1 >= 0.5) // + || (selectedSa1.getMateSupport() > 5 && + (selectedSa1.getExpectedDiscordants() - + selectedSa1.getMateSupport()) < 3)) { + if (mrefHits1 == 0 || mrefHits2 == 0) { + return 4; + } + } + } return 1; } - } - if ((selectedSa1.isFuzzy() || - selectedSa1.isStrictFuzzyCandidate())) { - if (evidenceLevel1 == 3 || + if (!hardClipSuspiciousCall && !selectedSa1.isStrictFuzzyCandidate()) { - if ((selectedSa1.getMateSupport() > 9 && - mateRatio1 >= 0.5) // - || (selectedSa1.getMateSupport() > 5 && - (selectedSa1.getExpectedDiscordants() - - selectedSa1.getMateSupport()) < 3)) { - if (mrefHits1 == 0 || mrefHits2 == 0) { - return 4; - } + if (selectedSa1.getSupport() > 4 || evidenceLevel1 == 3 || + selectedSa1.getMateSupport() > 9) { + return 4; } } - return 1; - } - if (!hardClipSuspiciousCall && - !selectedSa1.isStrictFuzzyCandidate()) { - if (selectedSa1.getSupport() > 4 || evidenceLevel1 == 3 || - selectedSa1.getMateSupport() > 9) { - return 4; - } - } - } - return 1; - } else if (inputScoreCategory == 2) { - // auto messageMode = selectedSa1.getChrIndex() == 11 && - //(selectedSa1.getPos() == 2261373 && selectedSa2.getPos() == 2148480); - if (!distant) { - if (totalEvidence1 < 5 && totalEvidence2 < 5) { - if (mrefHits1 > GERMLINEDBLIMIT || - mrefHits2 > GERMLINEDBLIMIT) { - return 1; - } - return 3; } - } else { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - if (selectedSa1.getSupport() > 30 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 10) { - eventType = 5; - return 2; - } - if (selectedSa1.getSupport() > 100 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 20) { - eventType = 5; - return 2; - } - if (selectedSa2.getSupport() > 30 && - selectedSa2.getSecondarySupport() < 10 && - selectedSa2.getMateSupport() < 10) { - eventType = 5; - return 2; - } - if (selectedSa2.getSupport() > 100 && - selectedSa2.getSecondarySupport() < 10 && - selectedSa2.getMateSupport() < 20) { - eventType = 5; - return 2; - } - } else if (mrefHits1 > GERMLINEDBLIMIT) { - if (selectedSa1.getSupport() > 50 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 10) { - eventType = 5; - return 2; - } - if (selectedSa1.getSupport() > 100 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 20) { - eventType = 5; - return 2; + return 1; + } else if (inputScoreCategory == 2) { + // auto messageMode = selectedSa1.getChrIndex() == 11 && + //(selectedSa1.getPos() == 2261373 && selectedSa2.getPos() == 2148480); + if (!distant) { + if (totalEvidence1 < 5 && totalEvidence2 < 5) { + if (mrefHits1 > GERMLINEDBLIMIT || + mrefHits2 > GERMLINEDBLIMIT) { + return 1; + } + return 3; } + } else { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + if (selectedSa1.getSupport() > 30 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 10) { + eventType = 5; + return 2; + } + if (selectedSa1.getSupport() > 100 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 20) { + eventType = 5; + return 2; + } + if (selectedSa2.getSupport() > 30 && + selectedSa2.getSecondarySupport() < 10 && + selectedSa2.getMateSupport() < 10) { + eventType = 5; + return 2; + } + if (selectedSa2.getSupport() > 100 && + selectedSa2.getSecondarySupport() < 10 && + selectedSa2.getMateSupport() < 20) { + eventType = 5; + return 2; + } + } else if (mrefHits1 > GERMLINEDBLIMIT) { + if (selectedSa1.getSupport() > 50 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 10) { + eventType = 5; + return 2; + } + if (selectedSa1.getSupport() > 100 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 20) { + eventType = 5; + return 2; + } - } else if (mrefHits2 > GERMLINEDBLIMIT) { - if (selectedSa2.getSupport() > 50 && - selectedSa2.getSecondarySupport() < 10 && - selectedSa2.getMateSupport() < 10) { - eventType = 5; - return 2; - } - if (selectedSa2.getSupport() > 100 && - selectedSa2.getSecondarySupport() < 10 && - selectedSa2.getMateSupport() < 20) { - eventType = 5; - return 2; - } - } - if (semiSuspicious) { - if (selectedSa1.isSemiSuspicious() && evidenceLevel1 < 3) { - return 2; + } else if (mrefHits2 > GERMLINEDBLIMIT) { + if (selectedSa2.getSupport() > 50 && + selectedSa2.getSecondarySupport() < 10 && + selectedSa2.getMateSupport() < 10) { + eventType = 5; + return 2; + } + if (selectedSa2.getSupport() > 100 && + selectedSa2.getSecondarySupport() < 10 && + selectedSa2.getMateSupport() < 20) { + eventType = 5; + return 2; + } } - if (selectedSa1.isSemiSuspicious() && - (selectedSa1.getSecondarySupport() == 0 || - selectedSa1.getSupport() == 0) && - evidenceLevel2 == 0) { - return 2; + if (semiSuspicious) { + if (selectedSa1.isSemiSuspicious() && evidenceLevel1 < 3) { + return 2; + } + if (selectedSa1.isSemiSuspicious() && + (selectedSa1.getSecondarySupport() == 0 || + selectedSa1.getSupport() == 0) && + evidenceLevel2 == 0) { + return 2; + } + if (selectedSa2.isSemiSuspicious()) { + if (evidenceLevel1 < 3) { + auto oneSidedScore = + assessEventScore(hardClipSuspiciousCall, 0); + if (oneSidedScore > 2) { + semiSuspicious = false; + return oneSidedScore; + } else { + return 2; + } + } + } } - if (selectedSa2.isSemiSuspicious()) { - if (evidenceLevel1 < 3) { - auto oneSidedScore = - assessEventScore(hardClipSuspiciousCall, 0); - if (oneSidedScore > 2) { - semiSuspicious = false; - return oneSidedScore; - } else { + if (selectedSa1.isFuzzy() && selectedSa2.isFuzzy()) { + if (!semiSuspicious && mrefHits1 == 0 && mrefHits2 == 0 && + !(selectedSa1.isProperPairErrorProne() || + selectedSa2.isProperPairErrorProne())) { + if (evidenceLevel1 > 1 || evidenceLevel2 > 1) { + if (mateRatio1 > 0.5 && mateRatio2 > 0.5) { + return 3; + } + } + } + if (evidenceLevel1 < 3 || evidenceLevel2 < 3) { + return 2; + } + } else { + if (selectedSa1.isFuzzy()) { + if (evidenceLevel1 < 2 && evidenceLevel2 < 2) { return 2; } } - } - } - if (selectedSa1.isFuzzy() && selectedSa2.isFuzzy()) { - if (!semiSuspicious && mrefHits1 == 0 && mrefHits2 == 0 && - !(selectedSa1.isProperPairErrorProne() || - selectedSa2.isProperPairErrorProne())) { - if (evidenceLevel1 > 1 || evidenceLevel2 > 1) { - if (mateRatio1 > 0.5 && mateRatio2 > 0.5) { - return 3; + if (selectedSa2.isFuzzy()) { + if (evidenceLevel2 < 2 && evidenceLevel1 < 2) { + return 2; } } } - if (evidenceLevel1 < 3 || evidenceLevel2 < 3) { - return 2; - } - } else { - if (selectedSa1.isFuzzy()) { - if (evidenceLevel1 < 2 && evidenceLevel2 < 2) { + if (evidenceLevel1 < 3 && evidenceLevel2 < 3) { + if ((selectedSa1.getMateSupport() < 10 && mateRatio1 < 0.5) || + (selectedSa2.getMateSupport() < 10 && mateRatio2 < 0.5)) { return 2; } - } - if (selectedSa2.isFuzzy()) { - if (evidenceLevel2 < 2 && evidenceLevel1 < 2) { + if (selectedSa1.getMateSupport() < 10 || + selectedSa2.getMateSupport() < 10) { + if ((mateRatio1 + mateRatio2) < 1.1) { + return 2; + } + } + if (!(evidenceLevel1 > 1 || evidenceLevel2 > 1)) { + if (!semiSuspicious && mrefHits1 == 0 && mrefHits2 == 0 && + !(selectedSa1.isProperPairErrorProne() || + selectedSa2.isProperPairErrorProne())) { + if (mateRatio1 >= 0.8 && mateRatio2 >= 0.8) { + if (selectedSa1.getMateSupport() > 4 && + selectedSa2.getMateSupport() > 4) { + if (selectedSa1.getMateSupport() > 9 || + selectedSa2.getMateSupport() > 9) { + return 3; + } + } + } + } return 2; } - } - } - if (evidenceLevel1 < 3 && evidenceLevel2 < 3) { - if ((selectedSa1.getMateSupport() < 10 && mateRatio1 < 0.5) || - (selectedSa2.getMateSupport() < 10 && mateRatio2 < 0.5)) { - return 2; - } - if (selectedSa1.getMateSupport() < 10 || - selectedSa2.getMateSupport() < 10) { - if ((mateRatio1 + mateRatio2) < 1.1) { + if (evidenceLevel2 == 1) { + if ((mateRatio2 >= 0.8 && + selectedSa2.getMateSupport() > 9)) { + auto oneSidedScore = + assessEventScore(hardClipSuspiciousCall, 0); + if (oneSidedScore > 2) { + return oneSidedScore; + } + } return 2; } - } - if (!(evidenceLevel1 > 1 || evidenceLevel2 > 1)) { - if (!semiSuspicious && mrefHits1 == 0 && mrefHits2 == 0 && + if (evidenceLevel1 == 1) { + if (mateRatio1 >= 0.8 && selectedSa1.getMateSupport() > 9 && + mateRatio2 >= 0.6) { + return 5; + } + return 2; + } + if (!semiSuspicious && mrefHits1 < GERMLINEDBLIMIT && + mrefHits2 < GERMLINEDBLIMIT && !(selectedSa1.isProperPairErrorProne() || selectedSa2.isProperPairErrorProne())) { if (mateRatio1 >= 0.8 && mateRatio2 >= 0.8) { if (selectedSa1.getMateSupport() > 4 && selectedSa2.getMateSupport() > 4) { - if (selectedSa1.getMateSupport() > 9 || - selectedSa2.getMateSupport() > 9) { - return 3; - } + return 5; } } } return 2; } - if (evidenceLevel2 == 1) { - if ((mateRatio2 >= 0.8 && - selectedSa2.getMateSupport() > 9)) { - auto oneSidedScore = - assessEventScore(hardClipSuspiciousCall, 0); - if (oneSidedScore > 2) { - return oneSidedScore; - } + } + return 5; + } else if (inputScoreCategory == 1) { + if (distant) { + if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { + if (selectedSa1.getSupport() > 30 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 10) { + eventType = 5; + return 1; } - return 2; - } - if (evidenceLevel1 == 1) { - if (mateRatio1 >= 0.8 && selectedSa1.getMateSupport() > 9 && - mateRatio2 >= 0.6) { - return 5; + if (selectedSa1.getSupport() > 100 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 20) { + eventType = 5; + return 1; } - return 2; - } - if (!semiSuspicious && mrefHits1 < GERMLINEDBLIMIT && - mrefHits2 < GERMLINEDBLIMIT && - !(selectedSa1.isProperPairErrorProne() || - selectedSa2.isProperPairErrorProne())) { - if (mateRatio1 >= 0.8 && mateRatio2 >= 0.8) { - if (selectedSa1.getMateSupport() > 4 && - selectedSa2.getMateSupport() > 4) { - return 5; - } + } else if (mrefHits1 > GERMLINEDBLIMIT) { + if (selectedSa1.getSupport() > 50 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 10) { + eventType = 5; + return 1; + } + if (selectedSa1.getSupport() > 100 && + selectedSa1.getSecondarySupport() < 10 && + selectedSa1.getMateSupport() < 20) { + eventType = 5; + return 1; } } - return 2; - } - } - return 5; - } else if (inputScoreCategory == 1) { - if (distant) { - if (mrefHits1 > GERMLINEDBLIMIT && mrefHits2 > GERMLINEDBLIMIT) { - if (selectedSa1.getSupport() > 30 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 10) { - eventType = 5; - return 1; - } - if (selectedSa1.getSupport() > 100 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 20) { - eventType = 5; - return 1; + if (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate()) { + if (evidenceLevel1 == 3) { + return 3; + } + return 0; } - } else if (mrefHits1 > GERMLINEDBLIMIT) { - if (selectedSa1.getSupport() > 50 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 10) { - eventType = 5; + } else { + if (totalEvidence1 < 5) { return 1; } - if (selectedSa1.getSupport() > 100 && - selectedSa1.getSecondarySupport() < 10 && - selectedSa1.getMateSupport() < 20) { - eventType = 5; + if (selectedSa1.getSupport() < 2 || + selectedSa1.getSecondarySupport() < 2) { return 1; } } - if (selectedSa1.isFuzzy() || selectedSa1.isStrictFuzzyCandidate()) { + if (!hardClipSuspiciousCall && !selectedSa1.isStrictFuzzyCandidate()) { if (evidenceLevel1 == 3) { return 3; + } else { + return 1; } - return 0; - } - } else { - if (totalEvidence1 < 5) { - return 1; - } - if (selectedSa1.getSupport() < 2 || - selectedSa1.getSecondarySupport() < 2) { - return 1; - } - } - if (!hardClipSuspiciousCall && !selectedSa1.isStrictFuzzyCandidate()) { - if (evidenceLevel1 == 3) { - return 3; - } else { - return 1; } + return 0; } return 0; } - return 0; -} -void -SvEvent::assessContamination(const vector> &overhangDb) { - if (eventType == 5) { - contaminationCandidate = 2; - return; - } - auto score1 = 0; - auto score2 = 0; - if (inputScore == 2) { - if (overhang2Index != -1) { - auto res = assessContaminationSingleBp(overhang2Index, overhangDb, - selectedSa2); - score2 = res.first; - overhang2lengthRatio = res.second; + void + SvEvent::assessContamination(const vector> &overhangDb) { + if (eventType == 5) { + contaminationCandidate = 2; + return; } - } - if (contaminationCandidate < 2) { - if (overhang1Index != -1) { - auto res = assessContaminationSingleBp(overhang1Index, overhangDb, - selectedSa1); - score1 = res.first; - overhang1lengthRatio = res.second; + auto score1 = 0; + auto score2 = 0; + if (inputScore == 2) { + if (overhang2Index != -1) { + auto res = assessContaminationSingleBp(overhang2Index, overhangDb, + selectedSa2); + score2 = res.first; + overhang2lengthRatio = res.second; + } } - } - if (score1 > 1 || score2 > 1) { - contaminationCandidate = 2; - } else if (score1 == 1 && score2 == 1) { - contaminationCandidate = 1; - } else if (score1 == 1 && score2 == 0) { - contaminationCandidate = 0; - } else if (score1 == 0 && score2 == 1) { - contaminationCandidate = 0; - } -} - -pair -SvEvent::assessContaminationSingleBp( - int overhangIndex, const vector> &overhangDb, - const SuppAlignmentAnno &selectedSa) { - auto overhangLengthMax = 0; - auto overhangLength = 0; - for (auto cit = overhangDb[overhangIndex].second.cbegin(); - cit != overhangDb[overhangIndex].second.cend(); ++cit) { - switch (*cit) { - case '(': - overhangLengthMax = max(overhangLengthMax, overhangLength); - overhangLength = 0; - break; - case ':': - overhangLength = 0; - break; - default: - ++overhangLength; - break; + if (contaminationCandidate < 2) { + if (overhang1Index != -1) { + auto res = assessContaminationSingleBp(overhang1Index, overhangDb, + selectedSa1); + score1 = res.first; + overhang1lengthRatio = res.second; + } + } + if (score1 > 1 || score2 > 1) { + contaminationCandidate = 2; + } else if (score1 == 1 && score2 == 1) { + contaminationCandidate = 1; + } else if (score1 == 1 && score2 == 0) { + contaminationCandidate = 0; + } else if (score1 == 0 && score2 == 1) { + contaminationCandidate = 0; } } - auto maxOverhangLengthRatio = - (overhangLengthMax + 0.0) / SuppAlignmentAnno::DEFAULTREADLENGTH; - if (selectedSa.getSecondarySupport() > 4) { - return {0, maxOverhangLengthRatio}; - } - if (maxOverhangLengthRatio > 0.7) { - if (selectedSa.getSecondarySupport() == 0) { - return {3, maxOverhangLengthRatio}; + + pair + SvEvent::assessContaminationSingleBp( + int overhangIndex, const vector> &overhangDb, + const SuppAlignmentAnno &selectedSa) { + auto overhangLengthMax = 0; + auto overhangLength = 0; + for (auto cit = overhangDb[overhangIndex].second.cbegin(); + cit != overhangDb[overhangIndex].second.cend(); ++cit) { + switch (*cit) { + case '(': + overhangLengthMax = max(overhangLengthMax, overhangLength); + overhangLength = 0; + break; + case ':': + overhangLength = 0; + break; + default: + ++overhangLength; + break; + } } - if (selectedSa.getSecondarySupport() < 3 || - (inputScore < 2 && selectedSa.getSecondarySupport() < 10)) { - return {2, maxOverhangLengthRatio}; + auto maxOverhangLengthRatio = + (overhangLengthMax + 0.0) / SuppAlignmentAnno::DEFAULTREADLENGTH; + if (selectedSa.getSecondarySupport() > 4) { + return {0, maxOverhangLengthRatio}; } - } else if (maxOverhangLengthRatio > 0.6) { - if (selectedSa.getSecondarySupport() < 3) { - return {1, maxOverhangLengthRatio}; + if (maxOverhangLengthRatio > 0.7) { + if (selectedSa.getSecondarySupport() == 0) { + return {3, maxOverhangLengthRatio}; + } + if (selectedSa.getSecondarySupport() < 3 || + (inputScore < 2 && selectedSa.getSecondarySupport() < 10)) { + return {2, maxOverhangLengthRatio}; + } + } else if (maxOverhangLengthRatio > 0.6) { + if (selectedSa.getSecondarySupport() < 3) { + return {1, maxOverhangLengthRatio}; + } } + return {0, maxOverhangLengthRatio}; } - return {0, maxOverhangLengthRatio}; -} -string -SvEvent::printMatch(const vector> &overhangDb) const { - vector outputFields; - outputFields.reserve(20); - const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); - outputFields.emplace_back(chrConverter.indexToChr[chrIndex1]); - outputFields.emplace_back(strtk::type_to_string(pos1 - 1)); - outputFields.emplace_back(strtk::type_to_string(pos1)); - outputFields.emplace_back(chrConverter.indexToChr[chrIndex2]); - outputFields.emplace_back(strtk::type_to_string(pos2 - 1)); - outputFields.emplace_back( - inputScore > 0 - ? strtk::type_to_string(pos2) - : strtk::type_to_string(selectedSa1.getExtendedPos())); - - if (!germlineStatus1) { + string + SvEvent::printMatch(const vector> &overhangDb) const { + vector outputFields; + outputFields.reserve(20); + const ChrConverter &chrConverter = GlobalAppConfig::getInstance().getChrConverter(); + outputFields.emplace_back(chrConverter.indexToChr[chrIndex1]); + outputFields.emplace_back(strtk::type_to_string(pos1 - 1)); + outputFields.emplace_back(strtk::type_to_string(pos1)); + outputFields.emplace_back(chrConverter.indexToChr[chrIndex2]); + outputFields.emplace_back(strtk::type_to_string(pos2 - 1)); outputFields.emplace_back( - "SOMATIC(" + strtk::type_to_string(mrefHits1) + "/" + - PIDSINMREFSTR + - "):" + boost::str(doubleFormatter % germlineClonality1)); - } else { - if (germline || (mrefHits1 > GERMLINEDBLIMIT)) { + inputScore > 0 + ? strtk::type_to_string(pos2) + : strtk::type_to_string(selectedSa1.getExtendedPos())); + + if (!germlineStatus1) { outputFields.emplace_back( - "GERMLINE(" + strtk::type_to_string(mrefHits1) + "/" + + "SOMATIC(" + strtk::type_to_string(mrefHits1) + "/" + PIDSINMREFSTR + "):" + boost::str(doubleFormatter % germlineClonality1)); } else { - outputFields.emplace_back( - "RESCUED(" + strtk::type_to_string(mrefHits1) + "/" + - PIDSINMREFSTR + - "):" + boost::str(doubleFormatter % germlineClonality1)); + if (germline || (mrefHits1 > GERMLINEDBLIMIT)) { + outputFields.emplace_back( + "GERMLINE(" + strtk::type_to_string(mrefHits1) + "/" + + PIDSINMREFSTR + + "):" + boost::str(doubleFormatter % germlineClonality1)); + } else { + outputFields.emplace_back( + "RESCUED(" + strtk::type_to_string(mrefHits1) + "/" + + PIDSINMREFSTR + + "):" + boost::str(doubleFormatter % germlineClonality1)); + } } - } - if (!germlineStatus2) { - if (inputScore > 0) { - outputFields.emplace_back( - "SOMATIC(" + strtk::type_to_string(mrefHits2) + "/" + - PIDSINMREFSTR + - "):" + boost::str(doubleFormatter % germlineClonality2)); + if (!germlineStatus2) { + if (inputScore > 0) { + outputFields.emplace_back( + "SOMATIC(" + strtk::type_to_string(mrefHits2) + "/" + + PIDSINMREFSTR + + "):" + boost::str(doubleFormatter % germlineClonality2)); + } else { + outputFields.emplace_back( + "UNKNOWN(" + strtk::type_to_string(mrefHits2) + "/" + + PIDSINMREFSTR + + "):" + boost::str(doubleFormatter % germlineClonality2)); + } + } else { - outputFields.emplace_back( - "UNKNOWN(" + strtk::type_to_string(mrefHits2) + "/" + - PIDSINMREFSTR + - "):" + boost::str(doubleFormatter % germlineClonality2)); + if (germline || (mrefHits2 > GERMLINEDBLIMIT)) { + outputFields.emplace_back( + "GERMLINE(" + strtk::type_to_string(mrefHits2) + "/" + + PIDSINMREFSTR + + "):" + boost::str(doubleFormatter % germlineClonality2)); + } else { + outputFields.emplace_back( + "RESCUED(" + strtk::type_to_string(mrefHits2) + "/" + + PIDSINMREFSTR + + "):" + boost::str(doubleFormatter % germlineClonality2)); + } } + outputFields.emplace_back(EVENTTYPES[eventType]); + outputFields.emplace_back((suspicious == 0) + ? strtk::type_to_string(eventScore) + : strtk::type_to_string(-suspicious)); + outputFields.emplace_back( + (eventSize > 0) ? strtk::type_to_string(eventSize) : "NA"); + outputFields.emplace_back(inverted ? "INV" : "NORMAL"); - } else { - if (germline || (mrefHits2 > GERMLINEDBLIMIT)) { + outputFields.emplace_back(strtk::type_to_string(totalEvidence1)); + outputFields.emplace_back(boost::str( + doubleFormatter % (totalEvidence1 / (totalEvidence1 + span1 + 0.0)))); + if (inputScore > 0) { + outputFields.emplace_back(strtk::type_to_string(totalEvidence2)); outputFields.emplace_back( - "GERMLINE(" + strtk::type_to_string(mrefHits2) + "/" + - PIDSINMREFSTR + - "):" + boost::str(doubleFormatter % germlineClonality2)); + (totalEvidence2 == 0 && span2 == 0) + ? "0.000" + : boost::str( + doubleFormatter % + (totalEvidence2 / (totalEvidence2 + span2 + 0.0)))); } else { - outputFields.emplace_back( - "RESCUED(" + strtk::type_to_string(mrefHits2) + "/" + - PIDSINMREFSTR + - "):" + boost::str(doubleFormatter % germlineClonality2)); + outputFields.emplace_back("UNKNOWN"); + outputFields.emplace_back("UNKNOWN"); } - } - outputFields.emplace_back(EVENTTYPES[eventType]); - outputFields.emplace_back((suspicious == 0) - ? strtk::type_to_string(eventScore) - : strtk::type_to_string(-suspicious)); - outputFields.emplace_back( - (eventSize > 0) ? strtk::type_to_string(eventSize) : "NA"); - outputFields.emplace_back(inverted ? "INV" : "NORMAL"); - - outputFields.emplace_back(strtk::type_to_string(totalEvidence1)); - outputFields.emplace_back(boost::str( - doubleFormatter % (totalEvidence1 / (totalEvidence1 + span1 + 0.0)))); - if (inputScore > 0) { - outputFields.emplace_back(strtk::type_to_string(totalEvidence2)); - outputFields.emplace_back( - (totalEvidence2 == 0 && span2 == 0) - ? "0.000" - : boost::str( - doubleFormatter % - (totalEvidence2 / (totalEvidence2 + span2 + 0.0)))); - } else { - outputFields.emplace_back("UNKNOWN"); - outputFields.emplace_back("UNKNOWN"); - } - outputFields.emplace_back(selectedSa1.print()); - outputFields.emplace_back(inputScore == 2 ? selectedSa2.print() : "_"); + outputFields.emplace_back(selectedSa1.print()); + outputFields.emplace_back(inputScore == 2 ? selectedSa2.print() : "_"); - outputFields.emplace_back( - overhang1Index != -1 ? overhangDb[overhang1Index].second : "."); - outputFields.emplace_back( - overhang2Index != -1 ? overhangDb[overhang2Index].second : "."); + outputFields.emplace_back( + overhang1Index != -1 ? overhangDb[overhang1Index].second : "."); + outputFields.emplace_back( + overhang2Index != -1 ? overhangDb[overhang2Index].second : "."); - return collapseRange(outputFields, "\t").append("\n"); -} -// vector SvEvent::getKey() const { -// if (!DEBUGMODE && (suspicious != 0 || eventScore == 0)) { -// return {}; -// } -// auto keyScore = (suspicious == 0) ? eventScore : suspicious; -// if (chrIndex1 < chrIndex2 || (chrIndex1 == chrIndex2 && pos1 < pos2)) { -// return {chrIndex1,pos1,chrIndex2,pos2,keyScore}; -// } else { -// return {chrIndex2,pos2,chrIndex1,pos1,keyScore}; -// } -// } + return collapseRange(outputFields, "\t").append("\n"); + } + // vector SvEvent::getKey() const { + // if (!DEBUGMODE && (suspicious != 0 || eventScore == 0)) { + // return {}; + // } + // auto keyScore = (suspicious == 0) ? eventScore : suspicious; + // if (chrIndex1 < chrIndex2 || (chrIndex1 == chrIndex2 && pos1 < pos2)) { + // return {chrIndex1,pos1,chrIndex2,pos2,keyScore}; + // } else { + // return {chrIndex2,pos2,chrIndex1,pos1,keyScore}; + // } + // } -string -SvEvent::getKey() const { - if (!DEBUGMODE && (suspicious != 0 || eventScore == 0)) { - return {}; - } - auto keyScore = (suspicious == 0) ? eventScore : suspicious; - if (chrIndex1 < chrIndex2 || (chrIndex1 == chrIndex2 && pos1 < pos2)) { - return collapseRange({to_string(chrIndex1), to_string(pos1), - to_string(chrIndex2), to_string(pos2), - to_string(keyScore)}, - "_"); - } else { - return collapseRange({to_string(chrIndex2), to_string(pos2), - to_string(chrIndex1), to_string(pos1), - to_string(keyScore)}, - "_"); + string + SvEvent::getKey() const { + if (!DEBUGMODE && (suspicious != 0 || eventScore == 0)) { + return {}; + } + auto keyScore = (suspicious == 0) ? eventScore : suspicious; + if (chrIndex1 < chrIndex2 || (chrIndex1 == chrIndex2 && pos1 < pos2)) { + return collapseRange({to_string(chrIndex1), to_string(pos1), + to_string(chrIndex2), to_string(pos2), + to_string(keyScore)}, + "_"); + } else { + return collapseRange({to_string(chrIndex2), to_string(pos2), + to_string(chrIndex1), to_string(pos1), + to_string(keyScore)}, + "_"); + } } -} } /* namespace sophia */ diff --git a/src/sophia.cpp b/src/sophia.cpp index 0ecbb21..36e051b 100644 --- a/src/sophia.cpp +++ b/src/sophia.cpp @@ -22,18 +22,17 @@ #include "GlobalAppConfig.h" -using namespace std; -using namespace sophia; - -pair getIsizeParameters(const string &ISIZEFILE); +std::pair getIsizeParameters(const std::string &ISIZEFILE); int main(int argc, char** argv) { - ios_base::sync_with_stdio(false); + using namespace sophia; + + std::ios_base::sync_with_stdio(false); cin.tie(nullptr); boost::program_options::options_description desc("Allowed options"); desc.add_options() // ("help", "produce help message") // - ("assemblyname", boost::program_options::value(), "assembly name (hg37, hg38)") // - ("mergedisizes", boost::program_options::value(), "insertsize distribution file for the merged bam") // + ("assemblyname", boost::program_options::value(), "assembly name (hg37, hg38)") // + ("mergedisizes", boost::program_options::value(), "insertsize distribution file for the merged bam") // ("medianisize", boost::program_options::value(), "median insert size for the merged bam") // ("stdisizepercentage", boost::program_options::value(), "percentage standard deviation of the insert size for the merged bam") // ("defaultreadlength", boost::program_options::value(), "Default read length for the technology used in sequencing 101,151 etc.") // @@ -99,9 +98,9 @@ int main(int argc, char** argv) { } } - string mergedIsizeFile; + std::string mergedIsizeFile; if (inputVariables.count("mergedisizes")) { - mergedIsizeFile = inputVariables["mergedisizes"].as(); + mergedIsizeFile = inputVariables["mergedisizes"].as(); auto isizeparams = getIsizeParameters(mergedIsizeFile); Alignment::ISIZEMAX = min(4000.0, isizeparams.first + isizeSigmaLevel * isizeparams.second); SuppAlignment::ISIZEMAX = Alignment::ISIZEMAX; @@ -124,12 +123,12 @@ int main(int argc, char** argv) { unique_ptr chrConverter; if (!inputVariables.count("assemblyname") || - inputVariables["assemblyname"].as() == Hg37ChrConverter::assembly_name) { + inputVariables["assemblyname"].as() == Hg37ChrConverter::assembly_name) { chrConverter = unique_ptr(new Hg37ChrConverter()); - } else if (inputVariables["assemblyname"].as() == Hg38ChrConverter::assembly_name) { + } else if (inputVariables["assemblyname"].as() == Hg38ChrConverter::assembly_name) { chrConverter = unique_ptr(new Hg38ChrConverter()); } else { - cerr << "Unknown assembly name " << inputVariables["assemblyname"].as() << ". I know " + cerr << "Unknown assembly name " << inputVariables["assemblyname"].as() << ". I know " << Hg37ChrConverter::assembly_name << " and " << Hg38ChrConverter::assembly_name << endl; return 1; @@ -156,12 +155,12 @@ int main(int argc, char** argv) { return 0; } -pair getIsizeParameters(const string &ISIZEFILE) { - pair isizeMedianStd { }; - ifstream infile { ISIZEFILE }; - string line; +std::pair getIsizeParameters(const std::string &ISIZEFILE) { + std::pair isizeMedianStd { }; + std::ifstream infile { ISIZEFILE }; + std::string line; auto i = 0; - while (error_terminating_getline(infile, line)) { + while (sophia::error_terminating_getline(infile, line)) { boost::algorithm::trim_right(line); switch (i) { case 0: diff --git a/src/sophiaAnnotate.cpp b/src/sophiaAnnotate.cpp index 194f7b9..a097b1c 100644 --- a/src/sophiaAnnotate.cpp +++ b/src/sophiaAnnotate.cpp @@ -28,7 +28,6 @@ int main(int argc, char** argv) { - using namespace std; using namespace sophia; ios_base::sync_with_stdio(false); diff --git a/src/sophiaMref.cpp b/src/sophiaMref.cpp index b31634a..dc48a1c 100644 --- a/src/sophiaMref.cpp +++ b/src/sophiaMref.cpp @@ -21,7 +21,6 @@ int main(int argc, char** argv) { - using namespace std; using namespace sophia; boost::program_options::options_description desc("Allowed options"); From 57f8be45f9df142807147b5fc449684d18d39951 Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Wed, 13 Dec 2023 13:33:54 +0100 Subject: [PATCH 06/79] Documentation for hg37 chromosome name parser. Signed-off-by: Philip R. Kensche --- include/ChrConverter.h | 3 ++- src/Hg37ChrConverter.cpp | 22 +++++++++++++++++++--- 2 files changed, 21 insertions(+), 4 deletions(-) diff --git a/include/ChrConverter.h b/include/ChrConverter.h index 71be479..a3cc126 100644 --- a/include/ChrConverter.h +++ b/include/ChrConverter.h @@ -59,7 +59,8 @@ namespace sophia { /** Mapping chromosome names to indices. */ const vector indexConverter; - /** Parse chromosome index. */ + /** Parse chromosome index. It takes a position in a character stream, and translates the + following character(s) into index positions (using ChrConverter::indexToChr). */ virtual int readChromosomeIndex(string::const_iterator startIt, char stopChar) const = 0; size_t n_chromosomes() { diff --git a/src/Hg37ChrConverter.cpp b/src/Hg37ChrConverter.cpp index d90bac8..792d60a 100644 --- a/src/Hg37ChrConverter.cpp +++ b/src/Hg37ChrConverter.cpp @@ -331,7 +331,23 @@ namespace sophia { hg37::indexConverter) {} /* This is parsing code. It takes a position in a character stream, and translates the - following character(s) into index positions (see ChrConverter::indexToChr). */ + following character(s) into index positions (see ChrConverter::indexToChr). + + If the first position is a digit, read up to the next stopChar. + If the first position is *not* a digit return indices according to the following rules: + + * h -> 999 + * X -> 40 + * Y -> 41 + * MT -> 1001 + * G?(\d+)\. -> \d+ + * N -> 1000 + * p -> 1002 + + NOTE: In none of these cases, the termination by a stopChar is actually checked. + + All identifiers not matching any of these rules, mapped to 1003. + */ int Hg37ChrConverter::readChromosomeIndex(std::string::const_iterator startIt, char stopChar) const { int chrIndex {0}; @@ -346,14 +362,14 @@ namespace sophia { return 999; case 'X': return 40; - case 'G': + case 'G': // Match GL...... chromosomes. for (auto cit = next(startIt, 2); *cit != '.'; ++cit) { chrIndex = 10 * chrIndex + *cit - '0'; } return chrIndex; case 'Y': return 41; - case 'M': + case 'M': // Match "MT" ++startIt; if (*startIt == 'T') { return 1001; From f4f4dce8a9391bdaed87aa6b8051fbe493f7475a Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Wed, 13 Dec 2023 13:41:13 +0100 Subject: [PATCH 07/79] Replace obscure -2 into NA variable to get more self-descriptive code. Signed-off-by: Philip R. Kensche --- src/Hg37ChrConverter.cpp | 104 +++++++++++++++++++------------------- src/Hg38ChrConverter.cpp | 105 ++++++++++++++++++++------------------- 2 files changed, 107 insertions(+), 102 deletions(-) diff --git a/src/Hg37ChrConverter.cpp b/src/Hg37ChrConverter.cpp index 792d60a..5eda8db 100644 --- a/src/Hg37ChrConverter.cpp +++ b/src/Hg37ChrConverter.cpp @@ -265,60 +265,62 @@ namespace sophia { 36652, 38155, 36423, 39787, 38503, 35477944, 171824}; + static const int NA = -2; + static const vector indexConverter { - -2, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, - 18, 19, 20, 21, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, 22, 23, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, -2, - -2, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, + NA, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, + 18, 19, 20, 21, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, 22, 23, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, + NA, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, - 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'0'); } return chrIndex; From cbcf2ddff5c9b4a962a687e4a72321a62e1a8c5f Mon Sep 17 00:00:00 2001 From: "Philip R. Kensche" Date: Fri, 15 Dec 2023 16:32:09 +0100 Subject: [PATCH 08/79] Major refactoring to have a map-based hg38 index/converter. * Added Makefile to replace build scripts. Signed-off-by: Philip R. Kensche --- GRCh38.contigInformation.selection.tsv | 811 ++++++ GRCh38.contigInformation.tsv | 3368 ++++++++++++++++++++++++ Makefile | 104 + README.md | 31 +- include/ChrConverter.h | 59 +- include/GlobalAppConfig.h | 6 +- include/Hg37ChrConverter.h | 51 +- include/Hg38ChrConverter.h | 64 +- include/MasterRefProcessor.h | 32 +- sophiaMref/makefile | 58 - sophiaMref/src/Alignment.d | 28 - sophiaMref/src/Alignment.o | Bin 303824 -> 0 bytes sophiaMref/src/AnnotationProcessor.d | 64 - sophiaMref/src/AnnotationProcessor.o | Bin 1143736 -> 0 bytes sophiaMref/src/Breakpoint.d | 31 - sophiaMref/src/Breakpoint.o | Bin 498200 -> 0 bytes sophiaMref/src/BreakpointReduced.d | 43 - sophiaMref/src/BreakpointReduced.o | Bin 489792 -> 0 bytes sophiaMref/src/ChosenBp.d | 10 - sophiaMref/src/ChosenBp.o | Bin 44608 -> 0 bytes sophiaMref/src/ChrConverter.d | 4 - sophiaMref/src/ChrConverter.o | Bin 179000 -> 0 bytes sophiaMref/src/DeFuzzier.d | 46 - sophiaMref/src/DeFuzzier.o | Bin 134600 -> 0 bytes sophiaMref/src/GermlineMatch.d | 13 - sophiaMref/src/GermlineMatch.o | Bin 37776 -> 0 bytes sophiaMref/src/MasterRefProcessor.d | 52 - sophiaMref/src/MasterRefProcessor.o | Bin 897168 -> 0 bytes sophiaMref/src/MrefEntry.d | 49 - sophiaMref/src/MrefEntry.o | Bin 650728 -> 0 bytes sophiaMref/src/MrefEntryAnno.d | 46 - sophiaMref/src/MrefEntryAnno.o | Bin 301992 -> 0 bytes sophiaMref/src/MrefMatch.d | 13 - sophiaMref/src/MrefMatch.o | Bin 19848 -> 0 bytes sophiaMref/src/SamSegmentMapper.d | 31 - sophiaMref/src/SamSegmentMapper.o | Bin 214240 -> 0 bytes sophiaMref/src/Sdust.d | 4 - sophiaMref/src/Sdust.o | Bin 93808 -> 0 bytes sophiaMref/src/SuppAlignment.d | 10 - sophiaMref/src/SuppAlignment.o | Bin 143632 -> 0 bytes sophiaMref/src/SuppAlignmentAnno.d | 13 - sophiaMref/src/SuppAlignmentAnno.o | Bin 98808 -> 0 bytes sophiaMref/src/SvEvent.d | 43 - sophiaMref/src/SvEvent.o | Bin 693432 -> 0 bytes sophiaMref/src/subdir.mk | 72 - src/Alignment.cpp | 4 +- src/AnnotationProcessor.cpp | 12 +- src/Breakpoint.cpp | 5 +- src/BreakpointReduced.cpp | 10 +- src/ChrConverter.cpp | 16 - src/GlobalAppConfig.cpp | 10 +- src/Hg37ChrConverter.cpp | 81 +- src/Hg38ChrConverter.cpp | 458 +--- src/MasterRefProcessor.cpp | 15 +- src/MrefEntry.cpp | 2 +- src/SuppAlignment.cpp | 8 +- src/SuppAlignmentAnno.cpp | 6 +- src/SvEvent.cpp | 16 +- src/sophia.cpp | 11 +- src/sophiaAnnotate.cpp | 11 +- src/sophiaMref.cpp | 10 +- 61 files changed, 4706 insertions(+), 1125 deletions(-) create mode 100644 GRCh38.contigInformation.selection.tsv create mode 100644 GRCh38.contigInformation.tsv create mode 100644 Makefile delete mode 100644 sophiaMref/makefile delete mode 100644 sophiaMref/src/Alignment.d delete mode 100644 sophiaMref/src/Alignment.o delete mode 100644 sophiaMref/src/AnnotationProcessor.d delete mode 100644 sophiaMref/src/AnnotationProcessor.o delete mode 100644 sophiaMref/src/Breakpoint.d delete mode 100644 sophiaMref/src/Breakpoint.o delete mode 100644 sophiaMref/src/BreakpointReduced.d delete mode 100644 sophiaMref/src/BreakpointReduced.o delete mode 100644 sophiaMref/src/ChosenBp.d delete mode 100644 sophiaMref/src/ChosenBp.o delete mode 100644 sophiaMref/src/ChrConverter.d delete mode 100644 sophiaMref/src/ChrConverter.o delete mode 100644 sophiaMref/src/DeFuzzier.d delete mode 100644 sophiaMref/src/DeFuzzier.o delete mode 100644 sophiaMref/src/GermlineMatch.d delete mode 100644 sophiaMref/src/GermlineMatch.o delete mode 100644 sophiaMref/src/MasterRefProcessor.d delete mode 100644 sophiaMref/src/MasterRefProcessor.o delete mode 100644 sophiaMref/src/MrefEntry.d delete mode 100644 sophiaMref/src/MrefEntry.o delete mode 100644 sophiaMref/src/MrefEntryAnno.d delete mode 100644 sophiaMref/src/MrefEntryAnno.o delete mode 100644 sophiaMref/src/MrefMatch.d delete mode 100644 sophiaMref/src/MrefMatch.o delete mode 100644 sophiaMref/src/SamSegmentMapper.d delete mode 100644 sophiaMref/src/SamSegmentMapper.o delete mode 100644 sophiaMref/src/Sdust.d delete mode 100644 sophiaMref/src/Sdust.o delete mode 100644 sophiaMref/src/SuppAlignment.d delete mode 100644 sophiaMref/src/SuppAlignment.o delete mode 100644 sophiaMref/src/SuppAlignmentAnno.d delete mode 100644 sophiaMref/src/SuppAlignmentAnno.o delete mode 100644 sophiaMref/src/SvEvent.d delete mode 100644 sophiaMref/src/SvEvent.o delete mode 100644 sophiaMref/src/subdir.mk diff --git a/GRCh38.contigInformation.selection.tsv b/GRCh38.contigInformation.selection.tsv new file mode 100644 index 0000000..fa3fd57 --- /dev/null +++ b/GRCh38.contigInformation.selection.tsv @@ -0,0 +1,811 @@ +chr1 248956422 TRUE +chr2 242193529 TRUE +chr3 198295559 TRUE +chr4 190214555 TRUE +chr5 181538259 TRUE +chr6 170805979 TRUE +chr7 159345973 TRUE +chr8 145138636 TRUE +chr9 138394717 TRUE +chr10 133797422 TRUE +chr11 135086622 TRUE +chr12 133275309 TRUE +chr13 114364328 TRUE +chr14 107043718 TRUE +chr15 101991189 TRUE +chr16 90338345 TRUE +chr17 83257441 TRUE +chr18 80373285 TRUE +chr19 58617616 TRUE +chr20 64444167 TRUE +chr21 46709983 TRUE +chr22 50818468 TRUE +chrX 156040895 TRUE +chrY 57227415 TRUE +chr1_KI270762v1_alt 354444 TRUE +chr1_KI270766v1_alt 256271 TRUE +chr1_KI270760v1_alt 109528 TRUE +chr1_KI270765v1_alt 185285 TRUE +chr1_GL383518v1_alt 182439 TRUE +chr1_GL383519v1_alt 110268 TRUE +chr1_GL383520v2_alt 366580 TRUE +chr1_KI270764v1_alt 50258 TRUE +chr1_KI270763v1_alt 911658 TRUE +chr1_KI270759v1_alt 425601 TRUE +chr1_KI270761v1_alt 165834 TRUE +chr2_KI270770v1_alt 136240 TRUE +chr2_KI270773v1_alt 70887 TRUE +chr2_KI270774v1_alt 223625 TRUE +chr2_KI270769v1_alt 120616 TRUE +chr2_GL383521v1_alt 143390 TRUE +chr2_KI270772v1_alt 133041 TRUE +chr2_KI270775v1_alt 138019 TRUE +chr2_KI270771v1_alt 110395 TRUE +chr2_KI270768v1_alt 110099 TRUE +chr2_GL582966v2_alt 96131 TRUE +chr2_GL383522v1_alt 123821 TRUE +chr2_KI270776v1_alt 174166 TRUE +chr2_KI270767v1_alt 161578 TRUE +chr3_JH636055v2_alt 173151 TRUE +chr3_KI270783v1_alt 109187 TRUE +chr3_KI270780v1_alt 224108 TRUE +chr3_GL383526v1_alt 180671 TRUE +chr3_KI270777v1_alt 173649 TRUE +chr3_KI270778v1_alt 248252 TRUE +chr3_KI270781v1_alt 113034 TRUE +chr3_KI270779v1_alt 205312 TRUE +chr3_KI270782v1_alt 162429 TRUE +chr3_KI270784v1_alt 184404 TRUE +chr4_KI270790v1_alt 220246 TRUE +chr4_GL383528v1_alt 376187 TRUE +chr4_KI270787v1_alt 111943 TRUE +chr4_GL000257v2_alt 586476 TRUE +chr4_KI270788v1_alt 158965 TRUE +chr4_GL383527v1_alt 164536 TRUE +chr4_KI270785v1_alt 119912 TRUE +chr4_KI270789v1_alt 205944 TRUE +chr4_KI270786v1_alt 244096 TRUE +chr5_KI270793v1_alt 126136 TRUE +chr5_KI270792v1_alt 179043 TRUE +chr5_KI270791v1_alt 195710 TRUE +chr5_GL383532v1_alt 82728 TRUE +chr5_GL949742v1_alt 226852 TRUE +chr5_KI270794v1_alt 164558 TRUE +chr5_GL339449v2_alt 1612928 TRUE +chr5_GL383530v1_alt 101241 TRUE +chr5_KI270796v1_alt 172708 TRUE +chr5_GL383531v1_alt 173459 TRUE +chr5_KI270795v1_alt 131892 TRUE +chr6_GL000250v2_alt 4672374 TRUE +chr6_KI270800v1_alt 175808 TRUE +chr6_KI270799v1_alt 152148 TRUE +chr6_GL383533v1_alt 124736 TRUE +chr6_KI270801v1_alt 870480 TRUE +chr6_KI270802v1_alt 75005 TRUE +chr6_KB021644v2_alt 185823 TRUE +chr6_KI270797v1_alt 197536 TRUE +chr6_KI270798v1_alt 271782 TRUE +chr7_KI270804v1_alt 157952 TRUE +chr7_KI270809v1_alt 209586 TRUE +chr7_KI270806v1_alt 158166 TRUE +chr7_GL383534v2_alt 119183 TRUE +chr7_KI270803v1_alt 1111570 TRUE +chr7_KI270808v1_alt 271455 TRUE +chr7_KI270807v1_alt 126434 TRUE +chr7_KI270805v1_alt 209988 TRUE +chr8_KI270818v1_alt 145606 TRUE +chr8_KI270812v1_alt 282736 TRUE +chr8_KI270811v1_alt 292436 TRUE +chr8_KI270821v1_alt 985506 TRUE +chr8_KI270813v1_alt 300230 TRUE +chr8_KI270822v1_alt 624492 TRUE +chr8_KI270814v1_alt 141812 TRUE +chr8_KI270810v1_alt 374415 TRUE +chr8_KI270819v1_alt 133535 TRUE +chr8_KI270820v1_alt 36640 TRUE +chr8_KI270817v1_alt 158983 TRUE +chr8_KI270816v1_alt 305841 TRUE +chr8_KI270815v1_alt 132244 TRUE +chr9_GL383539v1_alt 162988 TRUE +chr9_GL383540v1_alt 71551 TRUE +chr9_GL383541v1_alt 171286 TRUE +chr9_GL383542v1_alt 60032 TRUE +chr9_KI270823v1_alt 439082 TRUE +chr10_GL383545v1_alt 179254 TRUE +chr10_KI270824v1_alt 181496 TRUE +chr10_GL383546v1_alt 309802 TRUE +chr10_KI270825v1_alt 188315 TRUE +chr11_KI270832v1_alt 210133 TRUE +chr11_KI270830v1_alt 177092 TRUE +chr11_KI270831v1_alt 296895 TRUE +chr11_KI270829v1_alt 204059 TRUE +chr11_GL383547v1_alt 154407 TRUE +chr11_JH159136v1_alt 200998 TRUE +chr11_JH159137v1_alt 191409 TRUE +chr11_KI270827v1_alt 67707 TRUE +chr11_KI270826v1_alt 186169 TRUE +chr12_GL877875v1_alt 167313 TRUE +chr12_GL877876v1_alt 408271 TRUE +chr12_KI270837v1_alt 40090 TRUE +chr12_GL383549v1_alt 120804 TRUE +chr12_KI270835v1_alt 238139 TRUE +chr12_GL383550v2_alt 169178 TRUE +chr12_GL383552v1_alt 138655 TRUE +chr12_GL383553v2_alt 152874 TRUE +chr12_KI270834v1_alt 119498 TRUE +chr12_GL383551v1_alt 184319 TRUE +chr12_KI270833v1_alt 76061 TRUE +chr12_KI270836v1_alt 56134 TRUE +chr13_KI270840v1_alt 191684 TRUE +chr13_KI270839v1_alt 180306 TRUE +chr13_KI270843v1_alt 103832 TRUE +chr13_KI270841v1_alt 169134 TRUE +chr13_KI270838v1_alt 306913 TRUE +chr13_KI270842v1_alt 37287 TRUE +chr14_KI270844v1_alt 322166 TRUE +chr14_KI270847v1_alt 1511111 TRUE +chr14_KI270845v1_alt 180703 TRUE +chr14_KI270846v1_alt 1351393 TRUE +chr15_KI270852v1_alt 478999 TRUE +chr15_KI270851v1_alt 263054 TRUE +chr15_KI270848v1_alt 327382 TRUE +chr15_GL383554v1_alt 296527 TRUE +chr15_KI270849v1_alt 244917 TRUE +chr15_GL383555v2_alt 388773 TRUE +chr15_KI270850v1_alt 430880 TRUE +chr16_KI270854v1_alt 134193 TRUE +chr16_KI270856v1_alt 63982 TRUE +chr16_KI270855v1_alt 232857 TRUE +chr16_KI270853v1_alt 2659700 TRUE +chr16_GL383556v1_alt 192462 TRUE +chr16_GL383557v1_alt 89672 TRUE +chr17_GL383563v3_alt 375691 TRUE +chr17_KI270862v1_alt 391357 TRUE +chr17_KI270861v1_alt 196688 TRUE +chr17_KI270857v1_alt 2877074 TRUE +chr17_JH159146v1_alt 278131 TRUE +chr17_JH159147v1_alt 70345 TRUE +chr17_GL383564v2_alt 133151 TRUE +chr17_GL000258v2_alt 1821992 TRUE +chr17_GL383565v1_alt 223995 TRUE +chr17_KI270858v1_alt 235827 TRUE +chr17_KI270859v1_alt 108763 TRUE +chr17_GL383566v1_alt 90219 TRUE +chr17_KI270860v1_alt 178921 TRUE +chr18_KI270864v1_alt 111737 TRUE +chr18_GL383567v1_alt 289831 TRUE +chr18_GL383570v1_alt 164789 TRUE +chr18_GL383571v1_alt 198278 TRUE +chr18_GL383568v1_alt 104552 TRUE +chr18_GL383569v1_alt 167950 TRUE +chr18_GL383572v1_alt 159547 TRUE +chr18_KI270863v1_alt 167999 TRUE +chr19_KI270868v1_alt 61734 TRUE +chr19_KI270865v1_alt 52969 TRUE +chr19_GL383573v1_alt 385657 TRUE +chr19_GL383575v2_alt 170222 TRUE +chr19_GL383576v1_alt 188024 TRUE +chr19_GL383574v1_alt 155864 TRUE +chr19_KI270866v1_alt 43156 TRUE +chr19_KI270867v1_alt 233762 TRUE +chr19_GL949746v1_alt 987716 TRUE +chr20_GL383577v2_alt 128386 TRUE +chr20_KI270869v1_alt 118774 TRUE +chr20_KI270871v1_alt 58661 TRUE +chr20_KI270870v1_alt 183433 TRUE +chr21_GL383578v2_alt 63917 TRUE +chr21_KI270874v1_alt 166743 TRUE +chr21_KI270873v1_alt 143900 TRUE +chr21_GL383579v2_alt 201197 TRUE +chr21_GL383580v2_alt 74653 TRUE +chr21_GL383581v2_alt 116689 TRUE +chr21_KI270872v1_alt 82692 TRUE +chr22_KI270875v1_alt 259914 TRUE +chr22_KI270878v1_alt 186262 TRUE +chr22_KI270879v1_alt 304135 TRUE +chr22_KI270876v1_alt 263666 TRUE +chr22_KI270877v1_alt 101331 TRUE +chr22_GL383583v2_alt 96924 TRUE +chr22_GL383582v2_alt 162811 TRUE +chrX_KI270880v1_alt 284869 TRUE +chrX_KI270881v1_alt 144206 TRUE +chr19_KI270882v1_alt 248807 TRUE +chr19_KI270883v1_alt 170399 TRUE +chr19_KI270884v1_alt 157053 TRUE +chr19_KI270885v1_alt 171027 TRUE +chr19_KI270886v1_alt 204239 TRUE +chr19_KI270887v1_alt 209512 TRUE +chr19_KI270888v1_alt 155532 TRUE +chr19_KI270889v1_alt 170698 TRUE +chr19_KI270890v1_alt 184499 TRUE +chr19_KI270891v1_alt 170680 TRUE +chr1_KI270892v1_alt 162212 TRUE +chr2_KI270894v1_alt 214158 TRUE +chr2_KI270893v1_alt 161218 TRUE +chr3_KI270895v1_alt 162896 TRUE +chr4_KI270896v1_alt 378547 TRUE +chr5_KI270897v1_alt 1144418 TRUE +chr5_KI270898v1_alt 130957 TRUE +chr6_GL000251v2_alt 4795265 TRUE +chr7_KI270899v1_alt 190869 TRUE +chr8_KI270901v1_alt 136959 TRUE +chr8_KI270900v1_alt 318687 TRUE +chr11_KI270902v1_alt 106711 TRUE +chr11_KI270903v1_alt 214625 TRUE +chr12_KI270904v1_alt 572349 TRUE +chr15_KI270906v1_alt 196384 TRUE +chr15_KI270905v1_alt 5161414 TRUE +chr17_KI270907v1_alt 137721 TRUE +chr17_KI270910v1_alt 157099 TRUE +chr17_KI270909v1_alt 325800 TRUE +chr17_JH159148v1_alt 88070 TRUE +chr17_KI270908v1_alt 1423190 TRUE +chr18_KI270912v1_alt 174061 TRUE +chr18_KI270911v1_alt 157710 TRUE +chr19_GL949747v2_alt 729520 TRUE +chr22_KB663609v1_alt 74013 TRUE +chrX_KI270913v1_alt 274009 TRUE +chr19_KI270914v1_alt 205194 TRUE +chr19_KI270915v1_alt 170665 TRUE +chr19_KI270916v1_alt 184516 TRUE +chr19_KI270917v1_alt 190932 TRUE +chr19_KI270918v1_alt 123111 TRUE +chr19_KI270919v1_alt 170701 TRUE +chr19_KI270920v1_alt 198005 TRUE +chr19_KI270921v1_alt 282224 TRUE +chr19_KI270922v1_alt 187935 TRUE +chr19_KI270923v1_alt 189352 TRUE +chr3_KI270924v1_alt 166540 TRUE +chr4_KI270925v1_alt 555799 TRUE +chr6_GL000252v2_alt 4604811 TRUE +chr8_KI270926v1_alt 229282 TRUE +chr11_KI270927v1_alt 218612 TRUE +chr19_GL949748v2_alt 1064304 TRUE +chr22_KI270928v1_alt 176103 TRUE +chr19_KI270929v1_alt 186203 TRUE +chr19_KI270930v1_alt 200773 TRUE +chr19_KI270931v1_alt 170148 TRUE +chr19_KI270932v1_alt 215732 TRUE +chr19_KI270933v1_alt 170537 TRUE +chr19_GL000209v2_alt 177381 TRUE +chr3_KI270934v1_alt 163458 TRUE +chr6_GL000253v2_alt 4677643 TRUE +chr19_GL949749v2_alt 1091841 TRUE +chr3_KI270935v1_alt 197351 TRUE +chr6_GL000254v2_alt 4827813 TRUE +chr19_GL949750v2_alt 1066390 TRUE +chr3_KI270936v1_alt 164170 TRUE +chr6_GL000255v2_alt 4606388 TRUE +chr19_GL949751v2_alt 1002683 TRUE +chr3_KI270937v1_alt 165607 TRUE +chr6_GL000256v2_alt 4929269 TRUE +chr19_GL949752v1_alt 987100 TRUE +chr6_KI270758v1_alt 76752 TRUE +chr19_GL949753v2_alt 796479 TRUE +chr19_KI270938v1_alt 1066800 TRUE +chrEBV 171823 TRUE +HLA-A*01:01:01:01 3503 TRUE +HLA-A*01:01:01:02N 3291 TRUE 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3309 TRUE +HLA-A*02:89 3371 TRUE +HLA-A*02:95 3388 TRUE +HLA-A*03:01:01:01 3502 TRUE +HLA-A*03:01:01:02N 3373 TRUE +HLA-A*03:01:01:03 3094 TRUE +HLA-A*03:02:01 3502 TRUE +HLA-A*03:11N 3404 TRUE +HLA-A*03:21N 3095 TRUE +HLA-A*03:36N 3142 TRUE +HLA-A*11:01:01 3503 TRUE +HLA-A*11:01:18 3503 TRUE +HLA-A*11:02:01 3503 TRUE +HLA-A*11:05 3373 TRUE +HLA-A*11:110 2903 TRUE +HLA-A*11:25 3073 TRUE +HLA-A*11:50Q 3362 TRUE +HLA-A*11:60 3241 TRUE +HLA-A*11:69N 3500 TRUE +HLA-A*11:74 3227 TRUE +HLA-A*11:75 3184 TRUE +HLA-A*11:77 3233 TRUE +HLA-A*23:01:01 3502 TRUE +HLA-A*23:09 3104 TRUE +HLA-A*23:38N 3020 TRUE +HLA-A*24:02:01:01 3502 TRUE +HLA-A*24:02:01:02L 3502 TRUE +HLA-A*24:02:01:03 3075 TRUE +HLA-A*24:02:03Q 3247 TRUE +HLA-A*24:02:10 3356 TRUE +HLA-A*24:03:01 3502 TRUE +HLA-A*24:07:01 3502 TRUE +HLA-A*24:08 3502 TRUE +HLA-A*24:09N 3502 TRUE +HLA-A*24:10:01 3502 TRUE +HLA-A*24:11N 3503 TRUE +HLA-A*24:152 3176 TRUE +HLA-A*24:20 3502 TRUE +HLA-A*24:215 3116 TRUE +HLA-A*24:61 3043 TRUE +HLA-A*24:86N 3415 TRUE +HLA-A*25:01:01 2917 TRUE +HLA-A*26:01:01 3517 TRUE +HLA-A*26:11N 3091 TRUE +HLA-A*26:15 3217 TRUE +HLA-A*26:50 3141 TRUE +HLA-A*29:01:01:01 3518 TRUE +HLA-A*29:01:01:02N 3303 TRUE +HLA-A*29:02:01:01 3518 TRUE +HLA-A*29:02:01:02 3518 TRUE +HLA-A*29:46 3310 TRUE +HLA-A*30:01:01 3503 TRUE +HLA-A*30:02:01:01 2903 TRUE +HLA-A*30:02:01:02 3374 TRUE +HLA-A*30:04:01 3503 TRUE +HLA-A*30:89 2903 TRUE +HLA-A*31:01:02 3518 TRUE +HLA-A*31:01:23 2918 TRUE +HLA-A*31:04 2918 TRUE +HLA-A*31:14N 3090 TRUE +HLA-A*31:46 3075 TRUE +HLA-A*32:01:01 3518 TRUE +HLA-A*32:06 3389 TRUE +HLA-A*33:01:01 3518 TRUE +HLA-A*33:03:01 3518 TRUE +HLA-A*33:07 3389 TRUE +HLA-A*34:01:01 3517 TRUE +HLA-A*34:02:01 3096 TRUE +HLA-A*36:01 2903 TRUE +HLA-A*43:01 3388 TRUE +HLA-A*66:01:01 3517 TRUE +HLA-A*66:17 3075 TRUE +HLA-A*68:01:01:01 2930 TRUE +HLA-A*68:01:01:02 3517 TRUE +HLA-A*68:01:02:01 3517 TRUE +HLA-A*68:01:02:02 3388 TRUE +HLA-A*68:02:01:01 3517 TRUE +HLA-A*68:02:01:02 3506 TRUE +HLA-A*68:02:01:03 2909 TRUE 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+HLA-B*27:32 3325 TRUE +HLA-B*35:01:01:01 3327 TRUE +HLA-B*35:01:01:02 3327 TRUE +HLA-B*35:01:22 2806 TRUE +HLA-B*35:02:01 3327 TRUE +HLA-B*35:03:01 2689 TRUE +HLA-B*35:05:01 2690 TRUE +HLA-B*35:08:01 2689 TRUE +HLA-B*35:14:02 3327 TRUE +HLA-B*35:241 3042 TRUE +HLA-B*35:41 3327 TRUE +HLA-B*37:01:01 3324 TRUE +HLA-B*37:01:05 2687 TRUE +HLA-B*38:01:01 3312 TRUE +HLA-B*38:02:01 3312 TRUE +HLA-B*38:14 2738 TRUE +HLA-B*39:01:01:01 3155 TRUE +HLA-B*39:01:01:02L 3153 TRUE +HLA-B*39:01:01:03 3312 TRUE +HLA-B*39:01:03 3155 TRUE +HLA-B*39:01:16 3155 TRUE +HLA-B*39:01:21 3312 TRUE +HLA-B*39:05:01 2675 TRUE +HLA-B*39:06:02 2674 TRUE +HLA-B*39:10:01 3027 TRUE +HLA-B*39:13:02 3255 TRUE +HLA-B*39:14 2765 TRUE +HLA-B*39:34 3254 TRUE +HLA-B*39:38Q 2675 TRUE +HLA-B*40:01:01 2676 TRUE +HLA-B*40:01:02 3323 TRUE +HLA-B*40:02:01 3258 TRUE +HLA-B*40:03 2677 TRUE +HLA-B*40:06:01:01 3325 TRUE +HLA-B*40:06:01:02 3299 TRUE +HLA-B*40:10:01 3304 TRUE +HLA-B*40:150 2800 TRUE +HLA-B*40:40 2677 TRUE +HLA-B*40:72:01 3283 TRUE +HLA-B*40:79 3257 TRUE +HLA-B*41:01:01 3322 TRUE +HLA-B*41:02:01 3322 TRUE +HLA-B*42:01:01 3322 TRUE +HLA-B*42:02 2675 TRUE +HLA-B*42:08 3165 TRUE +HLA-B*44:02:01:01 3323 TRUE +HLA-B*44:02:01:02S 3152 TRUE +HLA-B*44:02:01:03 3152 TRUE +HLA-B*44:02:17 3323 TRUE +HLA-B*44:02:27 2872 TRUE +HLA-B*44:03:01 3323 TRUE +HLA-B*44:03:02 2676 TRUE +HLA-B*44:04 3239 TRUE +HLA-B*44:09 3317 TRUE +HLA-B*44:138Q 3043 TRUE +HLA-B*44:150 2676 TRUE +HLA-B*44:23N 3323 TRUE +HLA-B*44:26 2804 TRUE +HLA-B*44:46 3323 TRUE +HLA-B*44:49 3039 TRUE +HLA-B*44:56N 2676 TRUE +HLA-B*45:01:01 3338 TRUE +HLA-B*45:04 3339 TRUE +HLA-B*46:01:01 3336 TRUE +HLA-B*46:01:05 2891 TRUE +HLA-B*47:01:01:01 3041 TRUE +HLA-B*47:01:01:02 3041 TRUE +HLA-B*48:01:01 3323 TRUE +HLA-B*48:03:01 2676 TRUE +HLA-B*48:04 2676 TRUE +HLA-B*48:08 3323 TRUE +HLA-B*49:01:01 3340 TRUE +HLA-B*49:32 3340 TRUE +HLA-B*50:01:01 3340 TRUE +HLA-B*51:01:01 3327 TRUE +HLA-B*51:01:02 3043 TRUE +HLA-B*51:02:01 3327 TRUE +HLA-B*51:07:01 3327 TRUE +HLA-B*51:42 2962 TRUE +HLA-B*52:01:01:01 3327 TRUE +HLA-B*52:01:01:02 3327 TRUE +HLA-B*52:01:01:03 3327 TRUE +HLA-B*52:01:02 3327 TRUE +HLA-B*53:01:01 3327 TRUE +HLA-B*53:11 3274 TRUE +HLA-B*54:01:01 3332 TRUE +HLA-B*54:18 2813 TRUE +HLA-B*55:01:01 3332 TRUE +HLA-B*55:01:03 3332 TRUE +HLA-B*55:02:01 3333 TRUE +HLA-B*55:12 3332 TRUE +HLA-B*55:24 3332 TRUE +HLA-B*55:48 2980 TRUE +HLA-B*56:01:01 2688 TRUE +HLA-B*56:03 2688 TRUE +HLA-B*56:04 2688 TRUE +HLA-B*57:01:01 3337 TRUE +HLA-B*57:03:01 2689 TRUE +HLA-B*57:06 3284 TRUE +HLA-B*57:11 3306 TRUE +HLA-B*57:29 3337 TRUE +HLA-B*58:01:01 3336 TRUE +HLA-B*58:31N 3004 TRUE +HLA-B*59:01:01:01 3333 TRUE +HLA-B*59:01:01:02 3332 TRUE +HLA-B*67:01:01 3312 TRUE +HLA-B*67:01:02 2675 TRUE +HLA-B*67:02 3307 TRUE +HLA-B*73:01 3323 TRUE +HLA-B*78:01:01 3327 TRUE +HLA-B*81:01 2676 TRUE +HLA-B*82:02:01 3050 TRUE +HLA-C*01:02:01 3349 TRUE +HLA-C*01:02:11 3057 TRUE +HLA-C*01:02:29 3349 TRUE +HLA-C*01:02:30 3333 TRUE +HLA-C*01:03 3349 TRUE +HLA-C*01:06 2895 TRUE +HLA-C*01:08 3349 TRUE +HLA-C*01:14 2895 TRUE +HLA-C*01:21 2895 TRUE +HLA-C*01:30 3349 TRUE +HLA-C*01:40 2968 TRUE +HLA-C*02:02:02:01 3347 TRUE +HLA-C*02:02:02:02 3347 TRUE +HLA-C*02:10 2893 TRUE +HLA-C*02:11 3320 TRUE +HLA-C*02:16:02 3029 TRUE +HLA-C*02:69 2933 TRUE +HLA-C*02:85 3347 TRUE +HLA-C*02:86 3347 TRUE +HLA-C*02:87 3064 TRUE +HLA-C*03:02:01 2894 TRUE +HLA-C*03:02:02:01 3348 TRUE +HLA-C*03:02:02:02 2896 TRUE +HLA-C*03:02:02:03 3348 TRUE +HLA-C*03:03:01 3348 TRUE +HLA-C*03:04:01:01 3348 TRUE +HLA-C*03:04:01:02 3348 TRUE +HLA-C*03:04:02 2877 TRUE +HLA-C*03:04:04 2966 TRUE +HLA-C*03:05 2894 TRUE +HLA-C*03:06 2894 TRUE +HLA-C*03:100 3034 TRUE +HLA-C*03:13:01 3065 TRUE +HLA-C*03:20N 3321 TRUE +HLA-C*03:219 3070 TRUE +HLA-C*03:261 3348 TRUE +HLA-C*03:40:01 2894 TRUE +HLA-C*03:41:02 3328 TRUE +HLA-C*03:46 2997 TRUE +HLA-C*03:61 2894 TRUE +HLA-C*04:01:01:01 3349 TRUE +HLA-C*04:01:01:02 3349 TRUE +HLA-C*04:01:01:03 3349 TRUE +HLA-C*04:01:01:04 3012 TRUE +HLA-C*04:01:01:05 2931 TRUE +HLA-C*04:01:62 3329 TRUE +HLA-C*04:03:01 3349 TRUE +HLA-C*04:06 3349 TRUE +HLA-C*04:09N 2991 TRUE +HLA-C*04:128 3086 TRUE +HLA-C*04:161 3237 TRUE +HLA-C*04:177 3349 TRUE +HLA-C*04:70 3058 TRUE +HLA-C*04:71 3086 TRUE +HLA-C*05:01:01:01 3349 TRUE +HLA-C*05:01:01:02 3349 TRUE +HLA-C*05:08 3059 TRUE +HLA-C*05:09:01 3322 TRUE +HLA-C*05:93 2946 TRUE +HLA-C*06:02:01:01 3349 TRUE +HLA-C*06:02:01:02 3349 TRUE +HLA-C*06:02:01:03 3349 TRUE +HLA-C*06:23 3349 TRUE +HLA-C*06:24 3349 TRUE +HLA-C*06:46N 2987 TRUE +HLA-C*07:01:01:01 3354 TRUE +HLA-C*07:01:01:02 3093 TRUE +HLA-C*07:01:02 3352 TRUE +HLA-C*07:01:19 3354 TRUE +HLA-C*07:01:27 3195 TRUE +HLA-C*07:01:45 3354 TRUE +HLA-C*07:02:01:01 3354 TRUE +HLA-C*07:02:01:02 3074 TRUE +HLA-C*07:02:01:03 3354 TRUE +HLA-C*07:02:01:04 3353 TRUE +HLA-C*07:02:01:05 3354 TRUE +HLA-C*07:02:05 2903 TRUE +HLA-C*07:02:06 3354 TRUE +HLA-C*07:02:64 3354 TRUE +HLA-C*07:04:01 3354 TRUE +HLA-C*07:04:02 3343 TRUE +HLA-C*07:06 3354 TRUE +HLA-C*07:149 3098 TRUE +HLA-C*07:18 3353 TRUE +HLA-C*07:19 3222 TRUE +HLA-C*07:26 3069 TRUE +HLA-C*07:30 2903 TRUE +HLA-C*07:32N 3334 TRUE +HLA-C*07:384 3349 TRUE +HLA-C*07:385 3354 TRUE +HLA-C*07:386 3183 TRUE +HLA-C*07:391 3354 TRUE +HLA-C*07:392 3354 TRUE +HLA-C*07:49 2935 TRUE +HLA-C*07:56:02 3354 TRUE +HLA-C*07:66 3354 TRUE +HLA-C*07:67 3354 TRUE +HLA-C*08:01:01 3349 TRUE +HLA-C*08:01:03 2998 TRUE +HLA-C*08:02:01:01 3349 TRUE +HLA-C*08:02:01:02 3349 TRUE +HLA-C*08:03:01 3349 TRUE +HLA-C*08:04:01 2895 TRUE +HLA-C*08:112 3178 TRUE +HLA-C*08:20 3349 TRUE +HLA-C*08:21 3349 TRUE +HLA-C*08:22 3349 TRUE +HLA-C*08:24 2895 TRUE +HLA-C*08:27 3349 TRUE +HLA-C*08:36N 3097 TRUE +HLA-C*08:40 2978 TRUE +HLA-C*08:41 3019 TRUE +HLA-C*08:62 3086 TRUE +HLA-C*12:02:02 3349 TRUE +HLA-C*12:03:01:01 3349 TRUE +HLA-C*12:03:01:02 3348 TRUE +HLA-C*12:08 3066 TRUE +HLA-C*12:13 3058 TRUE +HLA-C*12:19 3349 TRUE +HLA-C*12:22 2895 TRUE +HLA-C*12:99 3349 TRUE +HLA-C*14:02:01 3349 TRUE +HLA-C*14:03 3349 TRUE +HLA-C*14:21N 3099 TRUE +HLA-C*14:23 2976 TRUE +HLA-C*15:02:01 3349 TRUE +HLA-C*15:05:01 3349 TRUE +HLA-C*15:05:02 3349 TRUE +HLA-C*15:13 2895 TRUE +HLA-C*15:16 3066 TRUE +HLA-C*15:17 3349 TRUE +HLA-C*15:96Q 3349 TRUE +HLA-C*16:01:01 3349 TRUE +HLA-C*16:02:01 2895 TRUE +HLA-C*16:04:01 3349 TRUE +HLA-C*17:01:01:01 3368 TRUE +HLA-C*17:01:01:02 3368 TRUE +HLA-C*17:01:01:03 3368 TRUE +HLA-C*17:03 3197 TRUE +HLA-C*18:01 3346 TRUE +HLA-DQA1*01:01:02 6489 TRUE +HLA-DQA1*01:02:01:01 6484 TRUE +HLA-DQA1*01:02:01:02 6485 TRUE +HLA-DQA1*01:02:01:03 6485 TRUE +HLA-DQA1*01:02:01:04 6492 TRUE +HLA-DQA1*01:03:01:01 6485 TRUE +HLA-DQA1*01:03:01:02 6492 TRUE +HLA-DQA1*01:04:01:01 6484 TRUE +HLA-DQA1*01:04:01:02 6485 TRUE +HLA-DQA1*01:05:01 6485 TRUE +HLA-DQA1*01:07 5959 TRUE +HLA-DQA1*01:10 5790 TRUE +HLA-DQA1*01:11 5926 TRUE +HLA-DQA1*02:01 6403 TRUE +HLA-DQA1*03:01:01 6437 TRUE +HLA-DQA1*03:02 6437 TRUE +HLA-DQA1*03:03:01 6437 TRUE +HLA-DQA1*04:01:02:01 5853 TRUE +HLA-DQA1*04:01:02:02 5666 TRUE +HLA-DQA1*04:02 6210 TRUE +HLA-DQA1*05:01:01:01 5806 TRUE +HLA-DQA1*05:01:01:02 6529 TRUE +HLA-DQA1*05:03 6121 TRUE +HLA-DQA1*05:05:01:01 6593 TRUE +HLA-DQA1*05:05:01:02 6597 TRUE +HLA-DQA1*05:05:01:03 6393 TRUE +HLA-DQA1*05:11 6589 TRUE +HLA-DQA1*06:01:01 5878 TRUE +HLA-DQB1*02:01:01 7480 TRUE +HLA-DQB1*02:02:01 7471 TRUE +HLA-DQB1*03:01:01:01 7231 TRUE +HLA-DQB1*03:01:01:02 7230 TRUE +HLA-DQB1*03:01:01:03 7231 TRUE +HLA-DQB1*03:02:01 7126 TRUE +HLA-DQB1*03:03:02:01 7126 TRUE +HLA-DQB1*03:03:02:02 7126 TRUE +HLA-DQB1*03:03:02:03 6800 TRUE +HLA-DQB1*03:05:01 6934 TRUE +HLA-DQB1*05:01:01:01 7090 TRUE +HLA-DQB1*05:01:01:02 7090 TRUE +HLA-DQB1*05:03:01:01 7089 TRUE +HLA-DQB1*05:03:01:02 7089 TRUE +HLA-DQB1*06:01:01 7111 TRUE +HLA-DQB1*06:02:01 7102 TRUE +HLA-DQB1*06:03:01 7103 TRUE +HLA-DQB1*06:09:01 7102 TRUE +HLA-DRB1*01:01:01 10741 TRUE +HLA-DRB1*01:02:01 11229 TRUE +HLA-DRB1*03:01:01:01 13908 TRUE +HLA-DRB1*03:01:01:02 13426 TRUE +HLA-DRB1*04:03:01 15246 TRUE +HLA-DRB1*07:01:01:01 16110 TRUE +HLA-DRB1*07:01:01:02 16120 TRUE +HLA-DRB1*08:03:02 13562 TRUE +HLA-DRB1*09:21 16039 TRUE +HLA-DRB1*10:01:01 13501 TRUE +HLA-DRB1*11:01:01 13921 TRUE +HLA-DRB1*11:01:02 13931 TRUE +HLA-DRB1*11:04:01 13919 TRUE +HLA-DRB1*12:01:01 13404 TRUE +HLA-DRB1*12:17 11260 TRUE +HLA-DRB1*13:01:01 13935 TRUE +HLA-DRB1*13:02:01 13941 TRUE +HLA-DRB1*14:05:01 13933 TRUE +HLA-DRB1*14:54:01 13936 TRUE +HLA-DRB1*15:01:01:01 11080 TRUE +HLA-DRB1*15:01:01:02 11571 TRUE +HLA-DRB1*15:01:01:03 11056 TRUE +HLA-DRB1*15:01:01:04 11056 TRUE +HLA-DRB1*15:02:01 10313 TRUE +HLA-DRB1*15:03:01:01 11567 TRUE +HLA-DRB1*15:03:01:02 11569 TRUE +HLA-DRB1*16:02:01 11005 TRUE diff --git a/GRCh38.contigInformation.tsv b/GRCh38.contigInformation.tsv new file mode 100644 index 0000000..7864c66 --- /dev/null +++ b/GRCh38.contigInformation.tsv @@ -0,0 +1,3368 @@ +chromosome size compressed_mref +chr1 248956422 TRUE +chr2 242193529 TRUE +chr3 198295559 TRUE +chr4 190214555 TRUE +chr5 181538259 TRUE +chr6 170805979 TRUE +chr7 159345973 TRUE +chr8 145138636 TRUE +chr9 138394717 TRUE +chr10 133797422 TRUE +chr11 135086622 TRUE +chr12 133275309 TRUE +chr13 114364328 TRUE +chr14 107043718 TRUE +chr15 101991189 TRUE +chr16 90338345 TRUE +chr17 83257441 TRUE +chr18 80373285 TRUE +chr19 58617616 TRUE +chr20 64444167 TRUE +chr21 46709983 TRUE +chr22 50818468 TRUE +chrX 156040895 TRUE +chrY 57227415 TRUE +chrM 16569 FALSE +chr1_KI270706v1_random 175055 FALSE +chr1_KI270707v1_random 32032 FALSE +chr1_KI270708v1_random 127682 FALSE +chr1_KI270709v1_random 66860 FALSE +chr1_KI270710v1_random 40176 FALSE +chr1_KI270711v1_random 42210 FALSE +chr1_KI270712v1_random 176043 FALSE +chr1_KI270713v1_random 40745 FALSE +chr1_KI270714v1_random 41717 FALSE +chr2_KI270715v1_random 161471 FALSE +chr2_KI270716v1_random 153799 FALSE +chr3_GL000221v1_random 155397 FALSE +chr4_GL000008v2_random 209709 FALSE +chr5_GL000208v1_random 92689 FALSE +chr9_KI270717v1_random 40062 FALSE +chr9_KI270718v1_random 38054 FALSE +chr9_KI270719v1_random 176845 FALSE +chr9_KI270720v1_random 39050 FALSE +chr11_KI270721v1_random 100316 FALSE +chr14_GL000009v2_random 201709 FALSE +chr14_GL000225v1_random 211173 FALSE +chr14_KI270722v1_random 194050 FALSE +chr14_GL000194v1_random 191469 FALSE +chr14_KI270723v1_random 38115 FALSE +chr14_KI270724v1_random 39555 FALSE +chr14_KI270725v1_random 172810 FALSE +chr14_KI270726v1_random 43739 FALSE +chr15_KI270727v1_random 448248 FALSE +chr16_KI270728v1_random 1872759 FALSE +chr17_GL000205v2_random 185591 FALSE +chr17_KI270729v1_random 280839 FALSE +chr17_KI270730v1_random 112551 FALSE +chr22_KI270731v1_random 150754 FALSE +chr22_KI270732v1_random 41543 FALSE +chr22_KI270733v1_random 179772 FALSE +chr22_KI270734v1_random 165050 FALSE +chr22_KI270735v1_random 42811 FALSE +chr22_KI270736v1_random 181920 FALSE +chr22_KI270737v1_random 103838 FALSE +chr22_KI270738v1_random 99375 FALSE +chr22_KI270739v1_random 73985 FALSE +chrY_KI270740v1_random 37240 FALSE +chrUn_KI270302v1 2274 FALSE +chrUn_KI270304v1 2165 FALSE +chrUn_KI270303v1 1942 FALSE +chrUn_KI270305v1 1472 FALSE +chrUn_KI270322v1 21476 FALSE +chrUn_KI270320v1 4416 FALSE +chrUn_KI270310v1 1201 FALSE +chrUn_KI270316v1 1444 FALSE +chrUn_KI270315v1 2276 FALSE +chrUn_KI270312v1 998 FALSE +chrUn_KI270311v1 12399 FALSE +chrUn_KI270317v1 37690 FALSE +chrUn_KI270412v1 1179 FALSE +chrUn_KI270411v1 2646 FALSE +chrUn_KI270414v1 2489 FALSE +chrUn_KI270419v1 1029 FALSE +chrUn_KI270418v1 2145 FALSE +chrUn_KI270420v1 2321 FALSE +chrUn_KI270424v1 2140 FALSE +chrUn_KI270417v1 2043 FALSE +chrUn_KI270422v1 1445 FALSE +chrUn_KI270423v1 981 FALSE +chrUn_KI270425v1 1884 FALSE +chrUn_KI270429v1 1361 FALSE +chrUn_KI270442v1 392061 FALSE +chrUn_KI270466v1 1233 FALSE +chrUn_KI270465v1 1774 FALSE +chrUn_KI270467v1 3920 FALSE +chrUn_KI270435v1 92983 FALSE +chrUn_KI270438v1 112505 FALSE +chrUn_KI270468v1 4055 FALSE +chrUn_KI270510v1 2415 FALSE +chrUn_KI270509v1 2318 FALSE +chrUn_KI270518v1 2186 FALSE +chrUn_KI270508v1 1951 FALSE +chrUn_KI270516v1 1300 FALSE +chrUn_KI270512v1 22689 FALSE +chrUn_KI270519v1 138126 FALSE +chrUn_KI270522v1 5674 FALSE +chrUn_KI270511v1 8127 FALSE +chrUn_KI270515v1 6361 FALSE +chrUn_KI270507v1 5353 FALSE +chrUn_KI270517v1 3253 FALSE +chrUn_KI270529v1 1899 FALSE +chrUn_KI270528v1 2983 FALSE +chrUn_KI270530v1 2168 FALSE +chrUn_KI270539v1 993 FALSE +chrUn_KI270538v1 91309 FALSE +chrUn_KI270544v1 1202 FALSE +chrUn_KI270548v1 1599 FALSE +chrUn_KI270583v1 1400 FALSE +chrUn_KI270587v1 2969 FALSE +chrUn_KI270580v1 1553 FALSE +chrUn_KI270581v1 7046 FALSE +chrUn_KI270579v1 31033 FALSE +chrUn_KI270589v1 44474 FALSE +chrUn_KI270590v1 4685 FALSE +chrUn_KI270584v1 4513 FALSE +chrUn_KI270582v1 6504 FALSE +chrUn_KI270588v1 6158 FALSE +chrUn_KI270593v1 3041 FALSE +chrUn_KI270591v1 5796 FALSE +chrUn_KI270330v1 1652 FALSE +chrUn_KI270329v1 1040 FALSE +chrUn_KI270334v1 1368 FALSE +chrUn_KI270333v1 2699 FALSE +chrUn_KI270335v1 1048 FALSE +chrUn_KI270338v1 1428 FALSE +chrUn_KI270340v1 1428 FALSE +chrUn_KI270336v1 1026 FALSE +chrUn_KI270337v1 1121 FALSE +chrUn_KI270363v1 1803 FALSE +chrUn_KI270364v1 2855 FALSE +chrUn_KI270362v1 3530 FALSE +chrUn_KI270366v1 8320 FALSE +chrUn_KI270378v1 1048 FALSE +chrUn_KI270379v1 1045 FALSE +chrUn_KI270389v1 1298 FALSE +chrUn_KI270390v1 2387 FALSE +chrUn_KI270387v1 1537 FALSE +chrUn_KI270395v1 1143 FALSE +chrUn_KI270396v1 1880 FALSE +chrUn_KI270388v1 1216 FALSE +chrUn_KI270394v1 970 FALSE +chrUn_KI270386v1 1788 FALSE +chrUn_KI270391v1 1484 FALSE +chrUn_KI270383v1 1750 FALSE +chrUn_KI270393v1 1308 FALSE +chrUn_KI270384v1 1658 FALSE +chrUn_KI270392v1 971 FALSE +chrUn_KI270381v1 1930 FALSE +chrUn_KI270385v1 990 FALSE +chrUn_KI270382v1 4215 FALSE +chrUn_KI270376v1 1136 FALSE +chrUn_KI270374v1 2656 FALSE +chrUn_KI270372v1 1650 FALSE +chrUn_KI270373v1 1451 FALSE +chrUn_KI270375v1 2378 FALSE +chrUn_KI270371v1 2805 FALSE +chrUn_KI270448v1 7992 FALSE +chrUn_KI270521v1 7642 FALSE +chrUn_GL000195v1 182896 FALSE +chrUn_GL000219v1 179198 FALSE +chrUn_GL000220v1 161802 FALSE +chrUn_GL000224v1 179693 FALSE +chrUn_KI270741v1 157432 FALSE +chrUn_GL000226v1 15008 FALSE +chrUn_GL000213v1 164239 FALSE +chrUn_KI270743v1 210658 FALSE +chrUn_KI270744v1 168472 FALSE +chrUn_KI270745v1 41891 FALSE +chrUn_KI270746v1 66486 FALSE +chrUn_KI270747v1 198735 FALSE +chrUn_KI270748v1 93321 FALSE +chrUn_KI270749v1 158759 FALSE +chrUn_KI270750v1 148850 FALSE +chrUn_KI270751v1 150742 FALSE +chrUn_KI270752v1 27745 FALSE +chrUn_KI270753v1 62944 FALSE +chrUn_KI270754v1 40191 FALSE +chrUn_KI270755v1 36723 FALSE +chrUn_KI270756v1 79590 FALSE +chrUn_KI270757v1 71251 FALSE +chrUn_GL000214v1 137718 FALSE +chrUn_KI270742v1 186739 FALSE +chrUn_GL000216v2 176608 FALSE +chrUn_GL000218v1 161147 FALSE +chr1_KI270762v1_alt 354444 TRUE +chr1_KI270766v1_alt 256271 TRUE +chr1_KI270760v1_alt 109528 TRUE +chr1_KI270765v1_alt 185285 TRUE +chr1_GL383518v1_alt 182439 TRUE +chr1_GL383519v1_alt 110268 TRUE +chr1_GL383520v2_alt 366580 TRUE +chr1_KI270764v1_alt 50258 TRUE +chr1_KI270763v1_alt 911658 TRUE +chr1_KI270759v1_alt 425601 TRUE +chr1_KI270761v1_alt 165834 TRUE +chr2_KI270770v1_alt 136240 TRUE +chr2_KI270773v1_alt 70887 TRUE +chr2_KI270774v1_alt 223625 TRUE +chr2_KI270769v1_alt 120616 TRUE +chr2_GL383521v1_alt 143390 TRUE +chr2_KI270772v1_alt 133041 TRUE +chr2_KI270775v1_alt 138019 TRUE +chr2_KI270771v1_alt 110395 TRUE +chr2_KI270768v1_alt 110099 TRUE +chr2_GL582966v2_alt 96131 TRUE +chr2_GL383522v1_alt 123821 TRUE +chr2_KI270776v1_alt 174166 TRUE +chr2_KI270767v1_alt 161578 TRUE +chr3_JH636055v2_alt 173151 TRUE +chr3_KI270783v1_alt 109187 TRUE +chr3_KI270780v1_alt 224108 TRUE +chr3_GL383526v1_alt 180671 TRUE +chr3_KI270777v1_alt 173649 TRUE +chr3_KI270778v1_alt 248252 TRUE +chr3_KI270781v1_alt 113034 TRUE +chr3_KI270779v1_alt 205312 TRUE +chr3_KI270782v1_alt 162429 TRUE +chr3_KI270784v1_alt 184404 TRUE +chr4_KI270790v1_alt 220246 TRUE +chr4_GL383528v1_alt 376187 TRUE +chr4_KI270787v1_alt 111943 TRUE +chr4_GL000257v2_alt 586476 TRUE +chr4_KI270788v1_alt 158965 TRUE +chr4_GL383527v1_alt 164536 TRUE +chr4_KI270785v1_alt 119912 TRUE +chr4_KI270789v1_alt 205944 TRUE +chr4_KI270786v1_alt 244096 TRUE +chr5_KI270793v1_alt 126136 TRUE +chr5_KI270792v1_alt 179043 TRUE +chr5_KI270791v1_alt 195710 TRUE +chr5_GL383532v1_alt 82728 TRUE +chr5_GL949742v1_alt 226852 TRUE +chr5_KI270794v1_alt 164558 TRUE +chr5_GL339449v2_alt 1612928 TRUE +chr5_GL383530v1_alt 101241 TRUE +chr5_KI270796v1_alt 172708 TRUE +chr5_GL383531v1_alt 173459 TRUE +chr5_KI270795v1_alt 131892 TRUE +chr6_GL000250v2_alt 4672374 TRUE +chr6_KI270800v1_alt 175808 TRUE +chr6_KI270799v1_alt 152148 TRUE +chr6_GL383533v1_alt 124736 TRUE +chr6_KI270801v1_alt 870480 TRUE +chr6_KI270802v1_alt 75005 TRUE +chr6_KB021644v2_alt 185823 TRUE +chr6_KI270797v1_alt 197536 TRUE +chr6_KI270798v1_alt 271782 TRUE +chr7_KI270804v1_alt 157952 TRUE +chr7_KI270809v1_alt 209586 TRUE +chr7_KI270806v1_alt 158166 TRUE +chr7_GL383534v2_alt 119183 TRUE +chr7_KI270803v1_alt 1111570 TRUE +chr7_KI270808v1_alt 271455 TRUE +chr7_KI270807v1_alt 126434 TRUE +chr7_KI270805v1_alt 209988 TRUE +chr8_KI270818v1_alt 145606 TRUE +chr8_KI270812v1_alt 282736 TRUE +chr8_KI270811v1_alt 292436 TRUE +chr8_KI270821v1_alt 985506 TRUE +chr8_KI270813v1_alt 300230 TRUE +chr8_KI270822v1_alt 624492 TRUE +chr8_KI270814v1_alt 141812 TRUE +chr8_KI270810v1_alt 374415 TRUE +chr8_KI270819v1_alt 133535 TRUE +chr8_KI270820v1_alt 36640 TRUE +chr8_KI270817v1_alt 158983 TRUE +chr8_KI270816v1_alt 305841 TRUE +chr8_KI270815v1_alt 132244 TRUE +chr9_GL383539v1_alt 162988 TRUE +chr9_GL383540v1_alt 71551 TRUE +chr9_GL383541v1_alt 171286 TRUE +chr9_GL383542v1_alt 60032 TRUE +chr9_KI270823v1_alt 439082 TRUE +chr10_GL383545v1_alt 179254 TRUE +chr10_KI270824v1_alt 181496 TRUE +chr10_GL383546v1_alt 309802 TRUE +chr10_KI270825v1_alt 188315 TRUE +chr11_KI270832v1_alt 210133 TRUE +chr11_KI270830v1_alt 177092 TRUE +chr11_KI270831v1_alt 296895 TRUE +chr11_KI270829v1_alt 204059 TRUE +chr11_GL383547v1_alt 154407 TRUE +chr11_JH159136v1_alt 200998 TRUE +chr11_JH159137v1_alt 191409 TRUE +chr11_KI270827v1_alt 67707 TRUE +chr11_KI270826v1_alt 186169 TRUE +chr12_GL877875v1_alt 167313 TRUE +chr12_GL877876v1_alt 408271 TRUE +chr12_KI270837v1_alt 40090 TRUE +chr12_GL383549v1_alt 120804 TRUE +chr12_KI270835v1_alt 238139 TRUE +chr12_GL383550v2_alt 169178 TRUE +chr12_GL383552v1_alt 138655 TRUE +chr12_GL383553v2_alt 152874 TRUE +chr12_KI270834v1_alt 119498 TRUE +chr12_GL383551v1_alt 184319 TRUE +chr12_KI270833v1_alt 76061 TRUE +chr12_KI270836v1_alt 56134 TRUE +chr13_KI270840v1_alt 191684 TRUE +chr13_KI270839v1_alt 180306 TRUE +chr13_KI270843v1_alt 103832 TRUE +chr13_KI270841v1_alt 169134 TRUE +chr13_KI270838v1_alt 306913 TRUE +chr13_KI270842v1_alt 37287 TRUE +chr14_KI270844v1_alt 322166 TRUE +chr14_KI270847v1_alt 1511111 TRUE +chr14_KI270845v1_alt 180703 TRUE +chr14_KI270846v1_alt 1351393 TRUE +chr15_KI270852v1_alt 478999 TRUE +chr15_KI270851v1_alt 263054 TRUE +chr15_KI270848v1_alt 327382 TRUE +chr15_GL383554v1_alt 296527 TRUE +chr15_KI270849v1_alt 244917 TRUE +chr15_GL383555v2_alt 388773 TRUE +chr15_KI270850v1_alt 430880 TRUE +chr16_KI270854v1_alt 134193 TRUE +chr16_KI270856v1_alt 63982 TRUE +chr16_KI270855v1_alt 232857 TRUE +chr16_KI270853v1_alt 2659700 TRUE +chr16_GL383556v1_alt 192462 TRUE +chr16_GL383557v1_alt 89672 TRUE +chr17_GL383563v3_alt 375691 TRUE +chr17_KI270862v1_alt 391357 TRUE +chr17_KI270861v1_alt 196688 TRUE +chr17_KI270857v1_alt 2877074 TRUE +chr17_JH159146v1_alt 278131 TRUE +chr17_JH159147v1_alt 70345 TRUE +chr17_GL383564v2_alt 133151 TRUE +chr17_GL000258v2_alt 1821992 TRUE +chr17_GL383565v1_alt 223995 TRUE +chr17_KI270858v1_alt 235827 TRUE +chr17_KI270859v1_alt 108763 TRUE +chr17_GL383566v1_alt 90219 TRUE +chr17_KI270860v1_alt 178921 TRUE +chr18_KI270864v1_alt 111737 TRUE +chr18_GL383567v1_alt 289831 TRUE +chr18_GL383570v1_alt 164789 TRUE +chr18_GL383571v1_alt 198278 TRUE +chr18_GL383568v1_alt 104552 TRUE +chr18_GL383569v1_alt 167950 TRUE +chr18_GL383572v1_alt 159547 TRUE +chr18_KI270863v1_alt 167999 TRUE +chr19_KI270868v1_alt 61734 TRUE +chr19_KI270865v1_alt 52969 TRUE +chr19_GL383573v1_alt 385657 TRUE +chr19_GL383575v2_alt 170222 TRUE +chr19_GL383576v1_alt 188024 TRUE +chr19_GL383574v1_alt 155864 TRUE +chr19_KI270866v1_alt 43156 TRUE +chr19_KI270867v1_alt 233762 TRUE +chr19_GL949746v1_alt 987716 TRUE +chr20_GL383577v2_alt 128386 TRUE +chr20_KI270869v1_alt 118774 TRUE +chr20_KI270871v1_alt 58661 TRUE +chr20_KI270870v1_alt 183433 TRUE +chr21_GL383578v2_alt 63917 TRUE +chr21_KI270874v1_alt 166743 TRUE +chr21_KI270873v1_alt 143900 TRUE +chr21_GL383579v2_alt 201197 TRUE +chr21_GL383580v2_alt 74653 TRUE +chr21_GL383581v2_alt 116689 TRUE +chr21_KI270872v1_alt 82692 TRUE +chr22_KI270875v1_alt 259914 TRUE +chr22_KI270878v1_alt 186262 TRUE +chr22_KI270879v1_alt 304135 TRUE +chr22_KI270876v1_alt 263666 TRUE +chr22_KI270877v1_alt 101331 TRUE +chr22_GL383583v2_alt 96924 TRUE +chr22_GL383582v2_alt 162811 TRUE +chrX_KI270880v1_alt 284869 TRUE +chrX_KI270881v1_alt 144206 TRUE +chr19_KI270882v1_alt 248807 TRUE +chr19_KI270883v1_alt 170399 TRUE +chr19_KI270884v1_alt 157053 TRUE +chr19_KI270885v1_alt 171027 TRUE +chr19_KI270886v1_alt 204239 TRUE +chr19_KI270887v1_alt 209512 TRUE +chr19_KI270888v1_alt 155532 TRUE +chr19_KI270889v1_alt 170698 TRUE +chr19_KI270890v1_alt 184499 TRUE +chr19_KI270891v1_alt 170680 TRUE +chr1_KI270892v1_alt 162212 TRUE +chr2_KI270894v1_alt 214158 TRUE +chr2_KI270893v1_alt 161218 TRUE +chr3_KI270895v1_alt 162896 TRUE +chr4_KI270896v1_alt 378547 TRUE +chr5_KI270897v1_alt 1144418 TRUE +chr5_KI270898v1_alt 130957 TRUE +chr6_GL000251v2_alt 4795265 TRUE +chr7_KI270899v1_alt 190869 TRUE +chr8_KI270901v1_alt 136959 TRUE +chr8_KI270900v1_alt 318687 TRUE +chr11_KI270902v1_alt 106711 TRUE +chr11_KI270903v1_alt 214625 TRUE +chr12_KI270904v1_alt 572349 TRUE +chr15_KI270906v1_alt 196384 TRUE +chr15_KI270905v1_alt 5161414 TRUE +chr17_KI270907v1_alt 137721 TRUE +chr17_KI270910v1_alt 157099 TRUE +chr17_KI270909v1_alt 325800 TRUE +chr17_JH159148v1_alt 88070 TRUE +chr17_KI270908v1_alt 1423190 TRUE +chr18_KI270912v1_alt 174061 TRUE +chr18_KI270911v1_alt 157710 TRUE +chr19_GL949747v2_alt 729520 TRUE +chr22_KB663609v1_alt 74013 TRUE +chrX_KI270913v1_alt 274009 TRUE +chr19_KI270914v1_alt 205194 TRUE +chr19_KI270915v1_alt 170665 TRUE +chr19_KI270916v1_alt 184516 TRUE +chr19_KI270917v1_alt 190932 TRUE +chr19_KI270918v1_alt 123111 TRUE +chr19_KI270919v1_alt 170701 TRUE +chr19_KI270920v1_alt 198005 TRUE +chr19_KI270921v1_alt 282224 TRUE +chr19_KI270922v1_alt 187935 TRUE +chr19_KI270923v1_alt 189352 TRUE +chr3_KI270924v1_alt 166540 TRUE +chr4_KI270925v1_alt 555799 TRUE +chr6_GL000252v2_alt 4604811 TRUE +chr8_KI270926v1_alt 229282 TRUE +chr11_KI270927v1_alt 218612 TRUE +chr19_GL949748v2_alt 1064304 TRUE +chr22_KI270928v1_alt 176103 TRUE +chr19_KI270929v1_alt 186203 TRUE +chr19_KI270930v1_alt 200773 TRUE +chr19_KI270931v1_alt 170148 TRUE +chr19_KI270932v1_alt 215732 TRUE +chr19_KI270933v1_alt 170537 TRUE +chr19_GL000209v2_alt 177381 TRUE +chr3_KI270934v1_alt 163458 TRUE +chr6_GL000253v2_alt 4677643 TRUE +chr19_GL949749v2_alt 1091841 TRUE +chr3_KI270935v1_alt 197351 TRUE +chr6_GL000254v2_alt 4827813 TRUE +chr19_GL949750v2_alt 1066390 TRUE +chr3_KI270936v1_alt 164170 TRUE +chr6_GL000255v2_alt 4606388 TRUE +chr19_GL949751v2_alt 1002683 TRUE +chr3_KI270937v1_alt 165607 TRUE +chr6_GL000256v2_alt 4929269 TRUE +chr19_GL949752v1_alt 987100 TRUE +chr6_KI270758v1_alt 76752 TRUE +chr19_GL949753v2_alt 796479 TRUE +chr19_KI270938v1_alt 1066800 TRUE +chrEBV 171823 TRUE +chrUn_KN707606v1_decoy 2200 FALSE +chrUn_KN707607v1_decoy 3033 FALSE +chrUn_KN707608v1_decoy 3112 FALSE +chrUn_KN707609v1_decoy 1642 FALSE +chrUn_KN707610v1_decoy 1393 FALSE +chrUn_KN707611v1_decoy 1103 FALSE +chrUn_KN707612v1_decoy 1039 FALSE +chrUn_KN707613v1_decoy 1619 FALSE +chrUn_KN707614v1_decoy 3122 FALSE +chrUn_KN707615v1_decoy 1934 FALSE +chrUn_KN707616v1_decoy 3111 FALSE +chrUn_KN707617v1_decoy 2545 FALSE +chrUn_KN707618v1_decoy 2295 FALSE +chrUn_KN707619v1_decoy 1551 FALSE +chrUn_KN707620v1_decoy 2046 FALSE +chrUn_KN707621v1_decoy 1222 FALSE +chrUn_KN707622v1_decoy 1535 FALSE +chrUn_KN707623v1_decoy 3784 FALSE +chrUn_KN707624v1_decoy 1329 FALSE +chrUn_KN707625v1_decoy 1238 FALSE +chrUn_KN707626v1_decoy 5623 FALSE +chrUn_KN707627v1_decoy 5821 FALSE +chrUn_KN707628v1_decoy 2960 FALSE +chrUn_KN707629v1_decoy 1848 FALSE +chrUn_KN707630v1_decoy 2315 FALSE +chrUn_KN707631v1_decoy 1945 FALSE +chrUn_KN707632v1_decoy 1424 FALSE +chrUn_KN707633v1_decoy 1274 FALSE +chrUn_KN707634v1_decoy 1007 FALSE +chrUn_KN707635v1_decoy 1414 FALSE +chrUn_KN707636v1_decoy 1725 FALSE +chrUn_KN707637v1_decoy 5354 FALSE +chrUn_KN707638v1_decoy 2189 FALSE +chrUn_KN707639v1_decoy 1294 FALSE +chrUn_KN707640v1_decoy 1831 FALSE +chrUn_KN707641v1_decoy 1647 FALSE +chrUn_KN707642v1_decoy 2943 FALSE +chrUn_KN707643v1_decoy 2857 FALSE +chrUn_KN707644v1_decoy 1030 FALSE +chrUn_KN707645v1_decoy 1070 FALSE +chrUn_KN707646v1_decoy 1735 FALSE +chrUn_KN707647v1_decoy 1982 FALSE +chrUn_KN707648v1_decoy 1564 FALSE +chrUn_KN707649v1_decoy 1775 FALSE +chrUn_KN707650v1_decoy 1540 FALSE +chrUn_KN707651v1_decoy 2013 FALSE +chrUn_KN707652v1_decoy 1176 FALSE +chrUn_KN707653v1_decoy 1890 FALSE +chrUn_KN707654v1_decoy 3644 FALSE +chrUn_KN707655v1_decoy 2785 FALSE +chrUn_KN707656v1_decoy 1017 FALSE +chrUn_KN707657v1_decoy 1068 FALSE +chrUn_KN707658v1_decoy 1007 FALSE +chrUn_KN707659v1_decoy 2605 FALSE +chrUn_KN707660v1_decoy 8410 FALSE +chrUn_KN707661v1_decoy 5534 FALSE +chrUn_KN707662v1_decoy 2173 FALSE +chrUn_KN707663v1_decoy 1065 FALSE +chrUn_KN707664v1_decoy 8683 FALSE +chrUn_KN707665v1_decoy 2670 FALSE +chrUn_KN707666v1_decoy 2420 FALSE +chrUn_KN707667v1_decoy 2189 FALSE +chrUn_KN707668v1_decoy 2093 FALSE +chrUn_KN707669v1_decoy 1184 FALSE +chrUn_KN707670v1_decoy 1205 FALSE +chrUn_KN707671v1_decoy 2786 FALSE +chrUn_KN707672v1_decoy 2794 FALSE +chrUn_KN707673v1_decoy 19544 FALSE +chrUn_KN707674v1_decoy 2848 FALSE +chrUn_KN707675v1_decoy 10556 FALSE +chrUn_KN707676v1_decoy 9066 FALSE +chrUn_KN707677v1_decoy 7267 FALSE +chrUn_KN707678v1_decoy 2462 FALSE +chrUn_KN707679v1_decoy 1774 FALSE +chrUn_KN707680v1_decoy 1297 FALSE +chrUn_KN707681v1_decoy 4379 FALSE +chrUn_KN707682v1_decoy 4208 FALSE +chrUn_KN707683v1_decoy 4068 FALSE +chrUn_KN707684v1_decoy 2940 FALSE +chrUn_KN707685v1_decoy 3938 FALSE +chrUn_KN707686v1_decoy 2072 FALSE +chrUn_KN707687v1_decoy 1136 FALSE +chrUn_KN707688v1_decoy 4248 FALSE +chrUn_KN707689v1_decoy 5823 FALSE +chrUn_KN707690v1_decoy 3715 FALSE +chrUn_KN707691v1_decoy 4885 FALSE +chrUn_KN707692v1_decoy 4813 FALSE +chrUn_KN707693v1_decoy 2899 FALSE +chrUn_KN707694v1_decoy 1228 FALSE +chrUn_KN707695v1_decoy 3119 FALSE +chrUn_KN707696v1_decoy 3828 FALSE +chrUn_KN707697v1_decoy 1186 FALSE +chrUn_KN707698v1_decoy 1908 FALSE +chrUn_KN707699v1_decoy 2795 FALSE +chrUn_KN707700v1_decoy 3703 FALSE +chrUn_KN707701v1_decoy 6722 FALSE +chrUn_KN707702v1_decoy 6466 FALSE +chrUn_KN707703v1_decoy 2235 FALSE +chrUn_KN707704v1_decoy 2871 FALSE +chrUn_KN707705v1_decoy 4632 FALSE +chrUn_KN707706v1_decoy 4225 FALSE +chrUn_KN707707v1_decoy 4339 FALSE +chrUn_KN707708v1_decoy 2305 FALSE +chrUn_KN707709v1_decoy 3273 FALSE +chrUn_KN707710v1_decoy 5701 FALSE +chrUn_KN707711v1_decoy 4154 FALSE +chrUn_KN707712v1_decoy 1243 FALSE +chrUn_KN707713v1_decoy 1308 FALSE +chrUn_KN707714v1_decoy 2922 FALSE +chrUn_KN707715v1_decoy 3044 FALSE +chrUn_KN707716v1_decoy 2888 FALSE +chrUn_KN707717v1_decoy 1742 FALSE +chrUn_KN707718v1_decoy 4969 FALSE +chrUn_KN707719v1_decoy 3270 FALSE +chrUn_KN707720v1_decoy 6028 FALSE +chrUn_KN707721v1_decoy 1105 FALSE +chrUn_KN707722v1_decoy 2884 FALSE +chrUn_KN707723v1_decoy 1124 FALSE +chrUn_KN707724v1_decoy 1454 FALSE +chrUn_KN707725v1_decoy 2565 FALSE +chrUn_KN707726v1_decoy 2149 FALSE +chrUn_KN707727v1_decoy 2630 FALSE +chrUn_KN707728v1_decoy 14625 FALSE +chrUn_KN707729v1_decoy 7431 FALSE +chrUn_KN707730v1_decoy 5776 FALSE +chrUn_KN707731v1_decoy 4820 FALSE +chrUn_KN707732v1_decoy 1227 FALSE +chrUn_KN707733v1_decoy 7503 FALSE +chrUn_KN707734v1_decoy 9652 FALSE +chrUn_KN707735v1_decoy 1091 FALSE +chrUn_KN707736v1_decoy 2467 FALSE +chrUn_KN707737v1_decoy 1270 FALSE +chrUn_KN707738v1_decoy 4365 FALSE +chrUn_KN707739v1_decoy 4284 FALSE +chrUn_KN707740v1_decoy 10282 FALSE +chrUn_KN707741v1_decoy 5601 FALSE +chrUn_KN707742v1_decoy 4758 FALSE +chrUn_KN707743v1_decoy 1624 FALSE +chrUn_KN707744v1_decoy 4024 FALSE +chrUn_KN707745v1_decoy 1276 FALSE +chrUn_KN707746v1_decoy 5083 FALSE +chrUn_KN707747v1_decoy 2075 FALSE +chrUn_KN707748v1_decoy 3553 FALSE +chrUn_KN707749v1_decoy 7010 FALSE +chrUn_KN707750v1_decoy 4718 FALSE +chrUn_KN707751v1_decoy 3546 FALSE +chrUn_KN707752v1_decoy 2873 FALSE +chrUn_KN707753v1_decoy 2144 FALSE +chrUn_KN707754v1_decoy 2243 FALSE +chrUn_KN707755v1_decoy 5343 FALSE +chrUn_KN707756v1_decoy 4877 FALSE +chrUn_KN707757v1_decoy 3034 FALSE +chrUn_KN707758v1_decoy 2826 FALSE +chrUn_KN707759v1_decoy 1221 FALSE +chrUn_KN707760v1_decoy 1169 FALSE +chrUn_KN707761v1_decoy 2319 FALSE +chrUn_KN707762v1_decoy 3450 FALSE +chrUn_KN707763v1_decoy 2674 FALSE +chrUn_KN707764v1_decoy 3912 FALSE +chrUn_KN707765v1_decoy 6020 FALSE +chrUn_KN707766v1_decoy 2303 FALSE +chrUn_KN707767v1_decoy 2552 FALSE +chrUn_KN707768v1_decoy 3656 FALSE +chrUn_KN707769v1_decoy 1591 FALSE +chrUn_KN707770v1_decoy 1209 FALSE +chrUn_KN707771v1_decoy 3176 FALSE +chrUn_KN707772v1_decoy 8915 FALSE +chrUn_KN707773v1_decoy 4902 FALSE +chrUn_KN707774v1_decoy 3324 FALSE +chrUn_KN707775v1_decoy 5997 FALSE +chrUn_KN707776v1_decoy 2618 FALSE +chrUn_KN707777v1_decoy 10311 FALSE +chrUn_KN707778v1_decoy 2440 FALSE +chrUn_KN707779v1_decoy 12444 FALSE +chrUn_KN707780v1_decoy 5691 FALSE +chrUn_KN707781v1_decoy 2717 FALSE +chrUn_KN707782v1_decoy 5277 FALSE +chrUn_KN707783v1_decoy 4373 FALSE +chrUn_KN707784v1_decoy 3224 FALSE +chrUn_KN707785v1_decoy 2631 FALSE +chrUn_KN707786v1_decoy 5385 FALSE +chrUn_KN707787v1_decoy 3678 FALSE +chrUn_KN707788v1_decoy 1412 FALSE +chrUn_KN707789v1_decoy 1443 FALSE +chrUn_KN707790v1_decoy 1098 FALSE +chrUn_KN707791v1_decoy 3240 FALSE +chrUn_KN707792v1_decoy 1915 FALSE +chrUn_KN707793v1_decoy 4667 FALSE +chrUn_KN707794v1_decoy 7219 FALSE +chrUn_KN707795v1_decoy 3277 FALSE +chrUn_KN707796v1_decoy 3473 FALSE +chrUn_KN707797v1_decoy 4243 FALSE +chrUn_KN707798v1_decoy 17599 FALSE +chrUn_KN707799v1_decoy 5095 FALSE +chrUn_KN707800v1_decoy 2237 FALSE +chrUn_KN707801v1_decoy 2901 FALSE +chrUn_KN707802v1_decoy 2666 FALSE +chrUn_KN707803v1_decoy 5336 FALSE +chrUn_KN707804v1_decoy 4383 FALSE +chrUn_KN707805v1_decoy 5446 FALSE +chrUn_KN707806v1_decoy 6252 FALSE +chrUn_KN707807v1_decoy 4616 FALSE +chrUn_KN707808v1_decoy 3021 FALSE +chrUn_KN707809v1_decoy 3667 FALSE +chrUn_KN707810v1_decoy 4563 FALSE +chrUn_KN707811v1_decoy 1120 FALSE +chrUn_KN707812v1_decoy 3845 FALSE +chrUn_KN707813v1_decoy 2272 FALSE +chrUn_KN707814v1_decoy 4764 FALSE +chrUn_KN707815v1_decoy 5410 FALSE +chrUn_KN707816v1_decoy 7150 FALSE +chrUn_KN707817v1_decoy 1762 FALSE +chrUn_KN707818v1_decoy 1207 FALSE +chrUn_KN707819v1_decoy 1331 FALSE +chrUn_KN707820v1_decoy 8307 FALSE +chrUn_KN707821v1_decoy 2276 FALSE +chrUn_KN707822v1_decoy 2575 FALSE +chrUn_KN707823v1_decoy 3970 FALSE +chrUn_KN707824v1_decoy 1352 FALSE +chrUn_KN707825v1_decoy 3040 FALSE +chrUn_KN707826v1_decoy 2070 FALSE +chrUn_KN707827v1_decoy 2913 FALSE +chrUn_KN707828v1_decoy 2389 FALSE +chrUn_KN707829v1_decoy 1835 FALSE +chrUn_KN707830v1_decoy 4807 FALSE +chrUn_KN707831v1_decoy 2201 FALSE +chrUn_KN707832v1_decoy 1265 FALSE +chrUn_KN707833v1_decoy 1961 FALSE +chrUn_KN707834v1_decoy 1064 FALSE +chrUn_KN707835v1_decoy 1932 FALSE +chrUn_KN707836v1_decoy 3213 FALSE +chrUn_KN707837v1_decoy 1178 FALSE +chrUn_KN707838v1_decoy 2926 FALSE +chrUn_KN707839v1_decoy 1038 FALSE +chrUn_KN707840v1_decoy 3298 FALSE +chrUn_KN707841v1_decoy 8992 FALSE +chrUn_KN707842v1_decoy 6698 FALSE +chrUn_KN707843v1_decoy 4880 FALSE +chrUn_KN707844v1_decoy 1766 FALSE +chrUn_KN707845v1_decoy 3532 FALSE +chrUn_KN707846v1_decoy 2297 FALSE +chrUn_KN707847v1_decoy 1234 FALSE +chrUn_KN707848v1_decoy 1205 FALSE +chrUn_KN707849v1_decoy 2790 FALSE +chrUn_KN707850v1_decoy 2006 FALSE +chrUn_KN707851v1_decoy 4593 FALSE +chrUn_KN707852v1_decoy 1579 FALSE +chrUn_KN707853v1_decoy 9597 FALSE +chrUn_KN707854v1_decoy 10451 FALSE +chrUn_KN707855v1_decoy 3219 FALSE +chrUn_KN707856v1_decoy 2300 FALSE +chrUn_KN707857v1_decoy 5985 FALSE +chrUn_KN707858v1_decoy 2959 FALSE +chrUn_KN707859v1_decoy 1340 FALSE +chrUn_KN707860v1_decoy 3148 FALSE +chrUn_KN707861v1_decoy 2242 FALSE +chrUn_KN707862v1_decoy 16513 FALSE +chrUn_KN707863v1_decoy 7821 FALSE +chrUn_KN707864v1_decoy 2159 FALSE +chrUn_KN707865v1_decoy 2114 FALSE +chrUn_KN707866v1_decoy 4109 FALSE +chrUn_KN707867v1_decoy 1544 FALSE +chrUn_KN707868v1_decoy 1005 FALSE +chrUn_KN707869v1_decoy 8632 FALSE +chrUn_KN707870v1_decoy 1012 FALSE +chrUn_KN707871v1_decoy 4728 FALSE +chrUn_KN707872v1_decoy 2165 FALSE +chrUn_KN707873v1_decoy 7591 FALSE +chrUn_KN707874v1_decoy 5202 FALSE +chrUn_KN707875v1_decoy 4241 FALSE +chrUn_KN707876v1_decoy 4131 FALSE +chrUn_KN707877v1_decoy 2272 FALSE +chrUn_KN707878v1_decoy 2085 FALSE +chrUn_KN707879v1_decoy 4346 FALSE +chrUn_KN707880v1_decoy 1208 FALSE +chrUn_KN707881v1_decoy 4543 FALSE +chrUn_KN707882v1_decoy 2772 FALSE +chrUn_KN707883v1_decoy 2490 FALSE +chrUn_KN707884v1_decoy 4568 FALSE +chrUn_KN707885v1_decoy 1776 FALSE +chrUn_KN707886v1_decoy 2699 FALSE +chrUn_KN707887v1_decoy 3534 FALSE +chrUn_KN707888v1_decoy 2424 FALSE +chrUn_KN707889v1_decoy 1747 FALSE +chrUn_KN707890v1_decoy 1088 FALSE +chrUn_KN707891v1_decoy 1143 FALSE +chrUn_KN707892v1_decoy 2530 FALSE +chrUn_KN707893v1_decoy 8049 FALSE +chrUn_KN707894v1_decoy 1366 FALSE +chrUn_KN707895v1_decoy 4284 FALSE +chrUn_KN707896v1_decoy 33125 FALSE +chrUn_KN707897v1_decoy 2137 FALSE +chrUn_KN707898v1_decoy 3840 FALSE +chrUn_KN707899v1_decoy 3087 FALSE +chrUn_KN707900v1_decoy 2041 FALSE +chrUn_KN707901v1_decoy 3344 FALSE +chrUn_KN707902v1_decoy 2921 FALSE +chrUn_KN707903v1_decoy 6581 FALSE +chrUn_KN707904v1_decoy 3968 FALSE +chrUn_KN707905v1_decoy 2339 FALSE +chrUn_KN707906v1_decoy 1243 FALSE +chrUn_KN707907v1_decoy 7776 FALSE +chrUn_KN707908v1_decoy 19837 FALSE +chrUn_KN707909v1_decoy 1737 FALSE +chrUn_KN707910v1_decoy 1098 FALSE +chrUn_KN707911v1_decoy 1893 FALSE +chrUn_KN707912v1_decoy 1281 FALSE +chrUn_KN707913v1_decoy 1527 FALSE +chrUn_KN707914v1_decoy 2055 FALSE +chrUn_KN707915v1_decoy 2527 FALSE +chrUn_KN707916v1_decoy 3275 FALSE +chrUn_KN707917v1_decoy 1265 FALSE +chrUn_KN707918v1_decoy 2623 FALSE +chrUn_KN707919v1_decoy 4850 FALSE +chrUn_KN707920v1_decoy 3584 FALSE +chrUn_KN707921v1_decoy 2561 FALSE +chrUn_KN707922v1_decoy 3041 FALSE +chrUn_KN707923v1_decoy 1409 FALSE +chrUn_KN707924v1_decoy 4596 FALSE +chrUn_KN707925v1_decoy 11555 FALSE +chrUn_KN707926v1_decoy 1266 FALSE +chrUn_KN707927v1_decoy 1079 FALSE +chrUn_KN707928v1_decoy 1087 FALSE +chrUn_KN707929v1_decoy 1226 FALSE +chrUn_KN707930v1_decoy 1131 FALSE +chrUn_KN707931v1_decoy 1199 FALSE +chrUn_KN707932v1_decoy 1084 FALSE +chrUn_KN707933v1_decoy 2038 FALSE +chrUn_KN707934v1_decoy 1070 FALSE +chrUn_KN707935v1_decoy 1312 FALSE +chrUn_KN707936v1_decoy 4031 FALSE +chrUn_KN707937v1_decoy 7445 FALSE +chrUn_KN707938v1_decoy 1770 FALSE +chrUn_KN707939v1_decoy 5600 FALSE +chrUn_KN707940v1_decoy 1882 FALSE +chrUn_KN707941v1_decoy 1170 FALSE +chrUn_KN707942v1_decoy 1300 FALSE +chrUn_KN707943v1_decoy 5325 FALSE +chrUn_KN707944v1_decoy 2043 FALSE +chrUn_KN707945v1_decoy 1072 FALSE +chrUn_KN707946v1_decoy 2463 FALSE +chrUn_KN707947v1_decoy 1010 FALSE +chrUn_KN707948v1_decoy 1432 FALSE +chrUn_KN707949v1_decoy 1162 FALSE +chrUn_KN707950v1_decoy 1095 FALSE +chrUn_KN707951v1_decoy 1118 FALSE +chrUn_KN707952v1_decoy 1383 FALSE +chrUn_KN707953v1_decoy 2289 FALSE +chrUn_KN707954v1_decoy 1648 FALSE +chrUn_KN707955v1_decoy 2203 FALSE +chrUn_KN707956v1_decoy 3270 FALSE +chrUn_KN707957v1_decoy 11499 FALSE +chrUn_KN707958v1_decoy 2474 FALSE +chrUn_KN707959v1_decoy 2294 FALSE +chrUn_KN707960v1_decoy 1238 FALSE +chrUn_KN707961v1_decoy 3410 FALSE +chrUn_KN707962v1_decoy 1523 FALSE +chrUn_KN707963v1_decoy 62955 FALSE +chrUn_KN707964v1_decoy 6282 FALSE +chrUn_KN707965v1_decoy 3836 FALSE +chrUn_KN707966v1_decoy 6486 FALSE +chrUn_KN707967v1_decoy 15368 FALSE +chrUn_KN707968v1_decoy 9572 FALSE +chrUn_KN707969v1_decoy 6413 FALSE +chrUn_KN707970v1_decoy 4104 FALSE +chrUn_KN707971v1_decoy 12943 FALSE +chrUn_KN707972v1_decoy 4650 FALSE +chrUn_KN707973v1_decoy 3080 FALSE +chrUn_KN707974v1_decoy 3134 FALSE +chrUn_KN707975v1_decoy 6211 FALSE +chrUn_KN707976v1_decoy 1126 FALSE +chrUn_KN707977v1_decoy 1101 FALSE +chrUn_KN707978v1_decoy 1101 FALSE +chrUn_KN707979v1_decoy 2648 FALSE +chrUn_KN707980v1_decoy 2973 FALSE 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8131 FALSE +chrUn_JTFH01000016v1_decoy 8051 FALSE +chrUn_JTFH01000017v1_decoy 7832 FALSE +chrUn_JTFH01000018v1_decoy 7710 FALSE +chrUn_JTFH01000019v1_decoy 7702 FALSE +chrUn_JTFH01000020v1_decoy 7479 FALSE +chrUn_JTFH01000021v1_decoy 7368 FALSE +chrUn_JTFH01000022v1_decoy 7162 FALSE +chrUn_JTFH01000023v1_decoy 7065 FALSE +chrUn_JTFH01000024v1_decoy 7019 FALSE +chrUn_JTFH01000025v1_decoy 6997 FALSE +chrUn_JTFH01000026v1_decoy 6994 FALSE +chrUn_JTFH01000027v1_decoy 6979 FALSE +chrUn_JTFH01000028v1_decoy 6797 FALSE +chrUn_JTFH01000029v1_decoy 6525 FALSE +chrUn_JTFH01000030v1_decoy 6246 FALSE +chrUn_JTFH01000031v1_decoy 5926 FALSE +chrUn_JTFH01000032v1_decoy 5914 FALSE +chrUn_JTFH01000033v1_decoy 5898 FALSE +chrUn_JTFH01000034v1_decoy 5879 FALSE +chrUn_JTFH01000035v1_decoy 5834 FALSE +chrUn_JTFH01000036v1_decoy 5743 FALSE +chrUn_JTFH01000037v1_decoy 5577 FALSE +chrUn_JTFH01000038v1_decoy 5413 FALSE +chrUn_JTFH01000039v1_decoy 5250 FALSE +chrUn_JTFH01000040v1_decoy 5246 FALSE 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+chrUn_JTFH01000391v1_decoy 1859 FALSE +chrUn_JTFH01000392v1_decoy 1856 FALSE +chrUn_JTFH01000393v1_decoy 1856 FALSE +chrUn_JTFH01000394v1_decoy 1854 FALSE +chrUn_JTFH01000395v1_decoy 1850 FALSE +chrUn_JTFH01000396v1_decoy 1849 FALSE +chrUn_JTFH01000397v1_decoy 1849 FALSE +chrUn_JTFH01000398v1_decoy 1847 FALSE +chrUn_JTFH01000399v1_decoy 1839 FALSE +chrUn_JTFH01000400v1_decoy 1834 FALSE +chrUn_JTFH01000401v1_decoy 1821 FALSE +chrUn_JTFH01000402v1_decoy 1815 FALSE +chrUn_JTFH01000403v1_decoy 1811 FALSE +chrUn_JTFH01000404v1_decoy 1808 FALSE +chrUn_JTFH01000405v1_decoy 1808 FALSE +chrUn_JTFH01000406v1_decoy 1807 FALSE +chrUn_JTFH01000407v1_decoy 1807 FALSE +chrUn_JTFH01000408v1_decoy 1802 FALSE +chrUn_JTFH01000409v1_decoy 1801 FALSE +chrUn_JTFH01000410v1_decoy 1800 FALSE +chrUn_JTFH01000411v1_decoy 1795 FALSE +chrUn_JTFH01000412v1_decoy 1794 FALSE +chrUn_JTFH01000413v1_decoy 1792 FALSE +chrUn_JTFH01000414v1_decoy 1788 FALSE +chrUn_JTFH01000415v1_decoy 1786 FALSE +chrUn_JTFH01000416v1_decoy 1782 FALSE +chrUn_JTFH01000417v1_decoy 1782 FALSE +chrUn_JTFH01000418v1_decoy 1781 FALSE +chrUn_JTFH01000419v1_decoy 1781 FALSE +chrUn_JTFH01000420v1_decoy 1779 FALSE +chrUn_JTFH01000421v1_decoy 1777 FALSE +chrUn_JTFH01000422v1_decoy 1764 FALSE +chrUn_JTFH01000423v1_decoy 1762 FALSE +chrUn_JTFH01000424v1_decoy 1755 FALSE +chrUn_JTFH01000425v1_decoy 1749 FALSE +chrUn_JTFH01000426v1_decoy 1747 FALSE +chrUn_JTFH01000427v1_decoy 1746 FALSE +chrUn_JTFH01000428v1_decoy 1745 FALSE +chrUn_JTFH01000429v1_decoy 1744 FALSE +chrUn_JTFH01000430v1_decoy 1742 FALSE +chrUn_JTFH01000431v1_decoy 1740 FALSE +chrUn_JTFH01000432v1_decoy 1740 FALSE +chrUn_JTFH01000433v1_decoy 1736 FALSE +chrUn_JTFH01000434v1_decoy 1735 FALSE +chrUn_JTFH01000435v1_decoy 1732 FALSE +chrUn_JTFH01000436v1_decoy 1732 FALSE +chrUn_JTFH01000437v1_decoy 1730 FALSE +chrUn_JTFH01000438v1_decoy 1727 FALSE +chrUn_JTFH01000439v1_decoy 1722 FALSE +chrUn_JTFH01000440v1_decoy 1718 FALSE +chrUn_JTFH01000441v1_decoy 1716 FALSE +chrUn_JTFH01000442v1_decoy 1710 FALSE +chrUn_JTFH01000443v1_decoy 1708 FALSE +chrUn_JTFH01000444v1_decoy 1707 FALSE +chrUn_JTFH01000445v1_decoy 1706 FALSE +chrUn_JTFH01000446v1_decoy 1705 FALSE +chrUn_JTFH01000447v1_decoy 1704 FALSE +chrUn_JTFH01000448v1_decoy 1699 FALSE +chrUn_JTFH01000449v1_decoy 1698 FALSE +chrUn_JTFH01000450v1_decoy 1697 FALSE +chrUn_JTFH01000451v1_decoy 1697 FALSE +chrUn_JTFH01000452v1_decoy 1695 FALSE +chrUn_JTFH01000453v1_decoy 1695 FALSE +chrUn_JTFH01000454v1_decoy 1693 FALSE +chrUn_JTFH01000455v1_decoy 1687 FALSE +chrUn_JTFH01000456v1_decoy 1686 FALSE +chrUn_JTFH01000457v1_decoy 1680 FALSE +chrUn_JTFH01000458v1_decoy 1679 FALSE +chrUn_JTFH01000459v1_decoy 1679 FALSE +chrUn_JTFH01000460v1_decoy 1678 FALSE +chrUn_JTFH01000461v1_decoy 1674 FALSE +chrUn_JTFH01000462v1_decoy 1674 FALSE +chrUn_JTFH01000463v1_decoy 1671 FALSE +chrUn_JTFH01000464v1_decoy 1669 FALSE +chrUn_JTFH01000465v1_decoy 1665 FALSE +chrUn_JTFH01000466v1_decoy 1663 FALSE +chrUn_JTFH01000467v1_decoy 1657 FALSE +chrUn_JTFH01000468v1_decoy 1653 FALSE +chrUn_JTFH01000469v1_decoy 1652 FALSE +chrUn_JTFH01000470v1_decoy 1650 FALSE +chrUn_JTFH01000471v1_decoy 1649 FALSE +chrUn_JTFH01000472v1_decoy 1649 FALSE +chrUn_JTFH01000473v1_decoy 1640 FALSE +chrUn_JTFH01000474v1_decoy 1638 FALSE +chrUn_JTFH01000475v1_decoy 1636 FALSE +chrUn_JTFH01000476v1_decoy 1632 FALSE +chrUn_JTFH01000477v1_decoy 1631 FALSE +chrUn_JTFH01000478v1_decoy 1630 FALSE +chrUn_JTFH01000479v1_decoy 1627 FALSE +chrUn_JTFH01000480v1_decoy 1624 FALSE +chrUn_JTFH01000481v1_decoy 1617 FALSE +chrUn_JTFH01000482v1_decoy 1616 FALSE +chrUn_JTFH01000483v1_decoy 1615 FALSE +chrUn_JTFH01000484v1_decoy 1611 FALSE +chrUn_JTFH01000485v1_decoy 1611 FALSE +chrUn_JTFH01000486v1_decoy 1606 FALSE +chrUn_JTFH01000487v1_decoy 1605 FALSE +chrUn_JTFH01000488v1_decoy 1605 FALSE +chrUn_JTFH01000489v1_decoy 1600 FALSE +chrUn_JTFH01000490v1_decoy 1598 FALSE 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+chrUn_JTFH01000516v1_decoy 1546 FALSE +chrUn_JTFH01000517v1_decoy 1541 FALSE +chrUn_JTFH01000518v1_decoy 1536 FALSE +chrUn_JTFH01000519v1_decoy 1533 FALSE +chrUn_JTFH01000520v1_decoy 1532 FALSE +chrUn_JTFH01000521v1_decoy 1532 FALSE +chrUn_JTFH01000522v1_decoy 1530 FALSE +chrUn_JTFH01000523v1_decoy 1527 FALSE +chrUn_JTFH01000524v1_decoy 1526 FALSE +chrUn_JTFH01000525v1_decoy 1524 FALSE +chrUn_JTFH01000526v1_decoy 1523 FALSE +chrUn_JTFH01000527v1_decoy 1523 FALSE +chrUn_JTFH01000528v1_decoy 1522 FALSE +chrUn_JTFH01000529v1_decoy 1522 FALSE +chrUn_JTFH01000530v1_decoy 1519 FALSE +chrUn_JTFH01000531v1_decoy 1513 FALSE +chrUn_JTFH01000532v1_decoy 1508 FALSE +chrUn_JTFH01000533v1_decoy 1508 FALSE +chrUn_JTFH01000534v1_decoy 1505 FALSE +chrUn_JTFH01000535v1_decoy 1503 FALSE +chrUn_JTFH01000536v1_decoy 1496 FALSE +chrUn_JTFH01000537v1_decoy 1491 FALSE +chrUn_JTFH01000538v1_decoy 1490 FALSE +chrUn_JTFH01000539v1_decoy 1490 FALSE +chrUn_JTFH01000540v1_decoy 1487 FALSE +chrUn_JTFH01000541v1_decoy 1486 FALSE +chrUn_JTFH01000542v1_decoy 1485 FALSE +chrUn_JTFH01000543v1_decoy 1484 FALSE +chrUn_JTFH01000544v1_decoy 1483 FALSE +chrUn_JTFH01000545v1_decoy 1479 FALSE +chrUn_JTFH01000546v1_decoy 1479 FALSE +chrUn_JTFH01000547v1_decoy 1476 FALSE +chrUn_JTFH01000548v1_decoy 1475 FALSE +chrUn_JTFH01000549v1_decoy 1472 FALSE +chrUn_JTFH01000550v1_decoy 1469 FALSE +chrUn_JTFH01000551v1_decoy 1468 FALSE +chrUn_JTFH01000552v1_decoy 1467 FALSE +chrUn_JTFH01000553v1_decoy 1465 FALSE +chrUn_JTFH01000554v1_decoy 1464 FALSE +chrUn_JTFH01000555v1_decoy 1463 FALSE +chrUn_JTFH01000556v1_decoy 1463 FALSE +chrUn_JTFH01000557v1_decoy 1459 FALSE +chrUn_JTFH01000558v1_decoy 1459 FALSE +chrUn_JTFH01000559v1_decoy 1458 FALSE +chrUn_JTFH01000560v1_decoy 1458 FALSE +chrUn_JTFH01000561v1_decoy 1454 FALSE +chrUn_JTFH01000562v1_decoy 1449 FALSE +chrUn_JTFH01000563v1_decoy 1449 FALSE +chrUn_JTFH01000564v1_decoy 1448 FALSE +chrUn_JTFH01000565v1_decoy 1446 FALSE 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+chrUn_JTFH01000616v1_decoy 1375 FALSE +chrUn_JTFH01000617v1_decoy 1374 FALSE +chrUn_JTFH01000618v1_decoy 1372 FALSE +chrUn_JTFH01000619v1_decoy 1371 FALSE +chrUn_JTFH01000620v1_decoy 1370 FALSE +chrUn_JTFH01000621v1_decoy 1370 FALSE +chrUn_JTFH01000622v1_decoy 1366 FALSE +chrUn_JTFH01000623v1_decoy 1363 FALSE +chrUn_JTFH01000624v1_decoy 1360 FALSE +chrUn_JTFH01000625v1_decoy 1356 FALSE +chrUn_JTFH01000626v1_decoy 1355 FALSE +chrUn_JTFH01000627v1_decoy 1355 FALSE +chrUn_JTFH01000628v1_decoy 1352 FALSE +chrUn_JTFH01000629v1_decoy 1345 FALSE +chrUn_JTFH01000630v1_decoy 1344 FALSE +chrUn_JTFH01000631v1_decoy 1344 FALSE +chrUn_JTFH01000632v1_decoy 1342 FALSE +chrUn_JTFH01000633v1_decoy 1342 FALSE +chrUn_JTFH01000634v1_decoy 1336 FALSE +chrUn_JTFH01000635v1_decoy 1334 FALSE +chrUn_JTFH01000636v1_decoy 1334 FALSE +chrUn_JTFH01000637v1_decoy 1333 FALSE +chrUn_JTFH01000638v1_decoy 1332 FALSE +chrUn_JTFH01000639v1_decoy 1328 FALSE +chrUn_JTFH01000640v1_decoy 1328 FALSE +chrUn_JTFH01000641v1_decoy 1328 FALSE +chrUn_JTFH01000642v1_decoy 1327 FALSE +chrUn_JTFH01000643v1_decoy 1325 FALSE +chrUn_JTFH01000644v1_decoy 1322 FALSE +chrUn_JTFH01000645v1_decoy 1320 FALSE +chrUn_JTFH01000646v1_decoy 1319 FALSE +chrUn_JTFH01000647v1_decoy 1318 FALSE +chrUn_JTFH01000648v1_decoy 1315 FALSE +chrUn_JTFH01000649v1_decoy 1314 FALSE +chrUn_JTFH01000650v1_decoy 1313 FALSE +chrUn_JTFH01000651v1_decoy 1313 FALSE +chrUn_JTFH01000652v1_decoy 1312 FALSE +chrUn_JTFH01000653v1_decoy 1310 FALSE +chrUn_JTFH01000654v1_decoy 1309 FALSE +chrUn_JTFH01000655v1_decoy 1309 FALSE +chrUn_JTFH01000656v1_decoy 1307 FALSE +chrUn_JTFH01000657v1_decoy 1307 FALSE +chrUn_JTFH01000658v1_decoy 1305 FALSE +chrUn_JTFH01000659v1_decoy 1304 FALSE +chrUn_JTFH01000660v1_decoy 1303 FALSE +chrUn_JTFH01000661v1_decoy 1302 FALSE +chrUn_JTFH01000662v1_decoy 1302 FALSE +chrUn_JTFH01000663v1_decoy 1301 FALSE +chrUn_JTFH01000664v1_decoy 1301 FALSE +chrUn_JTFH01000665v1_decoy 1300 FALSE +chrUn_JTFH01000666v1_decoy 1299 FALSE +chrUn_JTFH01000667v1_decoy 1297 FALSE +chrUn_JTFH01000668v1_decoy 1295 FALSE +chrUn_JTFH01000669v1_decoy 1294 FALSE +chrUn_JTFH01000670v1_decoy 1293 FALSE +chrUn_JTFH01000671v1_decoy 1291 FALSE +chrUn_JTFH01000672v1_decoy 1291 FALSE +chrUn_JTFH01000673v1_decoy 1289 FALSE +chrUn_JTFH01000674v1_decoy 1288 FALSE +chrUn_JTFH01000675v1_decoy 1288 FALSE +chrUn_JTFH01000676v1_decoy 1287 FALSE +chrUn_JTFH01000677v1_decoy 1287 FALSE +chrUn_JTFH01000678v1_decoy 1287 FALSE +chrUn_JTFH01000679v1_decoy 1286 FALSE +chrUn_JTFH01000680v1_decoy 1283 FALSE +chrUn_JTFH01000681v1_decoy 1281 FALSE +chrUn_JTFH01000682v1_decoy 1277 FALSE +chrUn_JTFH01000683v1_decoy 1274 FALSE +chrUn_JTFH01000684v1_decoy 1270 FALSE +chrUn_JTFH01000685v1_decoy 1267 FALSE +chrUn_JTFH01000686v1_decoy 1266 FALSE +chrUn_JTFH01000687v1_decoy 1260 FALSE +chrUn_JTFH01000688v1_decoy 1259 FALSE +chrUn_JTFH01000689v1_decoy 1258 FALSE +chrUn_JTFH01000690v1_decoy 1258 FALSE +chrUn_JTFH01000691v1_decoy 1258 FALSE +chrUn_JTFH01000692v1_decoy 1256 FALSE +chrUn_JTFH01000693v1_decoy 1255 FALSE +chrUn_JTFH01000694v1_decoy 1254 FALSE +chrUn_JTFH01000695v1_decoy 1254 FALSE +chrUn_JTFH01000696v1_decoy 1253 FALSE +chrUn_JTFH01000697v1_decoy 1250 FALSE +chrUn_JTFH01000698v1_decoy 1249 FALSE +chrUn_JTFH01000699v1_decoy 1248 FALSE +chrUn_JTFH01000700v1_decoy 1248 FALSE +chrUn_JTFH01000701v1_decoy 1247 FALSE +chrUn_JTFH01000702v1_decoy 1242 FALSE +chrUn_JTFH01000703v1_decoy 1242 FALSE +chrUn_JTFH01000704v1_decoy 1241 FALSE +chrUn_JTFH01000705v1_decoy 1241 FALSE +chrUn_JTFH01000706v1_decoy 1241 FALSE +chrUn_JTFH01000707v1_decoy 1239 FALSE +chrUn_JTFH01000708v1_decoy 1238 FALSE +chrUn_JTFH01000709v1_decoy 1237 FALSE +chrUn_JTFH01000710v1_decoy 1236 FALSE +chrUn_JTFH01000711v1_decoy 1235 FALSE +chrUn_JTFH01000712v1_decoy 1234 FALSE +chrUn_JTFH01000713v1_decoy 1234 FALSE +chrUn_JTFH01000714v1_decoy 1234 FALSE +chrUn_JTFH01000715v1_decoy 1233 FALSE +chrUn_JTFH01000716v1_decoy 1232 FALSE +chrUn_JTFH01000717v1_decoy 1232 FALSE +chrUn_JTFH01000718v1_decoy 1231 FALSE +chrUn_JTFH01000719v1_decoy 1230 FALSE +chrUn_JTFH01000720v1_decoy 1228 FALSE +chrUn_JTFH01000721v1_decoy 1227 FALSE +chrUn_JTFH01000722v1_decoy 1227 FALSE +chrUn_JTFH01000723v1_decoy 1226 FALSE +chrUn_JTFH01000724v1_decoy 1224 FALSE +chrUn_JTFH01000725v1_decoy 1224 FALSE +chrUn_JTFH01000726v1_decoy 1220 FALSE +chrUn_JTFH01000727v1_decoy 1220 FALSE +chrUn_JTFH01000728v1_decoy 1219 FALSE +chrUn_JTFH01000729v1_decoy 1217 FALSE +chrUn_JTFH01000730v1_decoy 1216 FALSE +chrUn_JTFH01000731v1_decoy 1215 FALSE +chrUn_JTFH01000732v1_decoy 1214 FALSE +chrUn_JTFH01000733v1_decoy 1214 FALSE +chrUn_JTFH01000734v1_decoy 1214 FALSE +chrUn_JTFH01000735v1_decoy 1213 FALSE +chrUn_JTFH01000736v1_decoy 1212 FALSE +chrUn_JTFH01000737v1_decoy 1209 FALSE +chrUn_JTFH01000738v1_decoy 1208 FALSE +chrUn_JTFH01000739v1_decoy 1207 FALSE +chrUn_JTFH01000740v1_decoy 1207 FALSE 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+chrUn_JTFH01000966v1_decoy 4041 FALSE +chrUn_JTFH01000967v1_decoy 3841 FALSE +chrUn_JTFH01000968v1_decoy 3754 FALSE +chrUn_JTFH01000969v1_decoy 3743 FALSE +chrUn_JTFH01000970v1_decoy 3702 FALSE +chrUn_JTFH01000971v1_decoy 3625 FALSE +chrUn_JTFH01000972v1_decoy 3529 FALSE +chrUn_JTFH01000973v1_decoy 3508 FALSE +chrUn_JTFH01000974v1_decoy 3359 FALSE +chrUn_JTFH01000975v1_decoy 3320 FALSE +chrUn_JTFH01000976v1_decoy 3231 FALSE +chrUn_JTFH01000977v1_decoy 3220 FALSE +chrUn_JTFH01000978v1_decoy 3212 FALSE +chrUn_JTFH01000979v1_decoy 3192 FALSE +chrUn_JTFH01000980v1_decoy 3092 FALSE +chrUn_JTFH01000981v1_decoy 3087 FALSE +chrUn_JTFH01000982v1_decoy 3048 FALSE +chrUn_JTFH01000983v1_decoy 3005 FALSE +chrUn_JTFH01000984v1_decoy 3004 FALSE +chrUn_JTFH01000985v1_decoy 2959 FALSE +chrUn_JTFH01000986v1_decoy 2934 FALSE +chrUn_JTFH01000987v1_decoy 2933 FALSE +chrUn_JTFH01000988v1_decoy 2827 FALSE +chrUn_JTFH01000989v1_decoy 2794 FALSE +chrUn_JTFH01000990v1_decoy 2749 FALSE 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+chrUn_JTFH01001016v1_decoy 2098 FALSE +chrUn_JTFH01001017v1_decoy 2066 FALSE +chrUn_JTFH01001018v1_decoy 2066 FALSE +chrUn_JTFH01001019v1_decoy 2059 FALSE +chrUn_JTFH01001020v1_decoy 2047 FALSE +chrUn_JTFH01001021v1_decoy 2040 FALSE +chrUn_JTFH01001022v1_decoy 2030 FALSE +chrUn_JTFH01001023v1_decoy 2024 FALSE +chrUn_JTFH01001024v1_decoy 2001 FALSE +chrUn_JTFH01001025v1_decoy 1992 FALSE +chrUn_JTFH01001026v1_decoy 1981 FALSE +chrUn_JTFH01001027v1_decoy 1979 FALSE +chrUn_JTFH01001028v1_decoy 1957 FALSE +chrUn_JTFH01001029v1_decoy 1953 FALSE +chrUn_JTFH01001030v1_decoy 1944 FALSE +chrUn_JTFH01001031v1_decoy 1936 FALSE +chrUn_JTFH01001032v1_decoy 1932 FALSE +chrUn_JTFH01001033v1_decoy 1882 FALSE +chrUn_JTFH01001034v1_decoy 1878 FALSE +chrUn_JTFH01001035v1_decoy 1870 FALSE +chrUn_JTFH01001036v1_decoy 1821 FALSE +chrUn_JTFH01001037v1_decoy 1813 FALSE +chrUn_JTFH01001038v1_decoy 1809 FALSE +chrUn_JTFH01001039v1_decoy 1804 FALSE +chrUn_JTFH01001040v1_decoy 1797 FALSE +chrUn_JTFH01001041v1_decoy 1791 FALSE +chrUn_JTFH01001042v1_decoy 1781 FALSE +chrUn_JTFH01001043v1_decoy 1766 FALSE +chrUn_JTFH01001044v1_decoy 1764 FALSE +chrUn_JTFH01001045v1_decoy 1743 FALSE +chrUn_JTFH01001046v1_decoy 1741 FALSE +chrUn_JTFH01001047v1_decoy 1709 FALSE +chrUn_JTFH01001048v1_decoy 1706 FALSE +chrUn_JTFH01001049v1_decoy 1701 FALSE +chrUn_JTFH01001050v1_decoy 1689 FALSE +chrUn_JTFH01001051v1_decoy 1646 FALSE +chrUn_JTFH01001052v1_decoy 1641 FALSE +chrUn_JTFH01001053v1_decoy 1639 FALSE +chrUn_JTFH01001054v1_decoy 1636 FALSE +chrUn_JTFH01001055v1_decoy 1632 FALSE +chrUn_JTFH01001056v1_decoy 1629 FALSE +chrUn_JTFH01001057v1_decoy 1623 FALSE +chrUn_JTFH01001058v1_decoy 1622 FALSE +chrUn_JTFH01001059v1_decoy 1622 FALSE +chrUn_JTFH01001060v1_decoy 1619 FALSE +chrUn_JTFH01001061v1_decoy 1606 FALSE +chrUn_JTFH01001062v1_decoy 1593 FALSE +chrUn_JTFH01001063v1_decoy 1592 FALSE +chrUn_JTFH01001064v1_decoy 1558 FALSE +chrUn_JTFH01001065v1_decoy 1545 FALSE +chrUn_JTFH01001066v1_decoy 1542 FALSE +chrUn_JTFH01001067v1_decoy 1540 FALSE +chrUn_JTFH01001068v1_decoy 1529 FALSE +chrUn_JTFH01001069v1_decoy 1518 FALSE +chrUn_JTFH01001070v1_decoy 1515 FALSE +chrUn_JTFH01001071v1_decoy 1513 FALSE +chrUn_JTFH01001072v1_decoy 1507 FALSE +chrUn_JTFH01001073v1_decoy 1504 FALSE +chrUn_JTFH01001074v1_decoy 1499 FALSE +chrUn_JTFH01001075v1_decoy 1495 FALSE +chrUn_JTFH01001076v1_decoy 1495 FALSE +chrUn_JTFH01001077v1_decoy 1492 FALSE +chrUn_JTFH01001078v1_decoy 1492 FALSE +chrUn_JTFH01001079v1_decoy 1489 FALSE +chrUn_JTFH01001080v1_decoy 1485 FALSE +chrUn_JTFH01001081v1_decoy 1483 FALSE +chrUn_JTFH01001082v1_decoy 1473 FALSE +chrUn_JTFH01001083v1_decoy 1470 FALSE +chrUn_JTFH01001084v1_decoy 1463 FALSE +chrUn_JTFH01001085v1_decoy 1460 FALSE +chrUn_JTFH01001086v1_decoy 1458 FALSE +chrUn_JTFH01001087v1_decoy 1456 FALSE +chrUn_JTFH01001088v1_decoy 1453 FALSE +chrUn_JTFH01001089v1_decoy 1443 FALSE +chrUn_JTFH01001090v1_decoy 1441 FALSE +chrUn_JTFH01001091v1_decoy 1426 FALSE +chrUn_JTFH01001092v1_decoy 1425 FALSE +chrUn_JTFH01001093v1_decoy 1418 FALSE +chrUn_JTFH01001094v1_decoy 1413 FALSE +chrUn_JTFH01001095v1_decoy 1413 FALSE +chrUn_JTFH01001096v1_decoy 1412 FALSE +chrUn_JTFH01001097v1_decoy 1407 FALSE +chrUn_JTFH01001098v1_decoy 1406 FALSE +chrUn_JTFH01001099v1_decoy 1396 FALSE +chrUn_JTFH01001100v1_decoy 1390 FALSE +chrUn_JTFH01001101v1_decoy 1382 FALSE +chrUn_JTFH01001102v1_decoy 1376 FALSE +chrUn_JTFH01001103v1_decoy 1375 FALSE +chrUn_JTFH01001104v1_decoy 1371 FALSE +chrUn_JTFH01001105v1_decoy 1367 FALSE +chrUn_JTFH01001106v1_decoy 1364 FALSE +chrUn_JTFH01001107v1_decoy 1356 FALSE +chrUn_JTFH01001108v1_decoy 1355 FALSE +chrUn_JTFH01001109v1_decoy 1352 FALSE +chrUn_JTFH01001110v1_decoy 1350 FALSE +chrUn_JTFH01001111v1_decoy 1346 FALSE +chrUn_JTFH01001112v1_decoy 1345 FALSE +chrUn_JTFH01001113v1_decoy 1340 FALSE +chrUn_JTFH01001114v1_decoy 1330 FALSE +chrUn_JTFH01001115v1_decoy 1329 FALSE +chrUn_JTFH01001116v1_decoy 1324 FALSE +chrUn_JTFH01001117v1_decoy 1316 FALSE +chrUn_JTFH01001118v1_decoy 1307 FALSE +chrUn_JTFH01001119v1_decoy 1304 FALSE +chrUn_JTFH01001120v1_decoy 1304 FALSE +chrUn_JTFH01001121v1_decoy 1303 FALSE +chrUn_JTFH01001122v1_decoy 1301 FALSE +chrUn_JTFH01001123v1_decoy 1300 FALSE +chrUn_JTFH01001124v1_decoy 1297 FALSE +chrUn_JTFH01001125v1_decoy 1296 FALSE +chrUn_JTFH01001126v1_decoy 1290 FALSE +chrUn_JTFH01001127v1_decoy 1284 FALSE +chrUn_JTFH01001128v1_decoy 1282 FALSE +chrUn_JTFH01001129v1_decoy 1281 FALSE +chrUn_JTFH01001130v1_decoy 1280 FALSE +chrUn_JTFH01001131v1_decoy 1279 FALSE +chrUn_JTFH01001132v1_decoy 1272 FALSE +chrUn_JTFH01001133v1_decoy 1267 FALSE +chrUn_JTFH01001134v1_decoy 1267 FALSE +chrUn_JTFH01001135v1_decoy 1266 FALSE +chrUn_JTFH01001136v1_decoy 1264 FALSE +chrUn_JTFH01001137v1_decoy 1264 FALSE +chrUn_JTFH01001138v1_decoy 1264 FALSE +chrUn_JTFH01001139v1_decoy 1263 FALSE +chrUn_JTFH01001140v1_decoy 1249 FALSE 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+chrUn_JTFH01001166v1_decoy 1169 FALSE +chrUn_JTFH01001167v1_decoy 1167 FALSE +chrUn_JTFH01001168v1_decoy 1166 FALSE +chrUn_JTFH01001169v1_decoy 1165 FALSE +chrUn_JTFH01001170v1_decoy 1164 FALSE +chrUn_JTFH01001171v1_decoy 1163 FALSE +chrUn_JTFH01001172v1_decoy 1158 FALSE +chrUn_JTFH01001173v1_decoy 1158 FALSE +chrUn_JTFH01001174v1_decoy 1157 FALSE +chrUn_JTFH01001175v1_decoy 1157 FALSE +chrUn_JTFH01001176v1_decoy 1157 FALSE +chrUn_JTFH01001177v1_decoy 1155 FALSE +chrUn_JTFH01001178v1_decoy 1154 FALSE +chrUn_JTFH01001179v1_decoy 1149 FALSE +chrUn_JTFH01001180v1_decoy 1148 FALSE +chrUn_JTFH01001181v1_decoy 1148 FALSE +chrUn_JTFH01001182v1_decoy 1146 FALSE +chrUn_JTFH01001183v1_decoy 1144 FALSE +chrUn_JTFH01001184v1_decoy 1140 FALSE +chrUn_JTFH01001185v1_decoy 1136 FALSE +chrUn_JTFH01001186v1_decoy 1134 FALSE +chrUn_JTFH01001187v1_decoy 1133 FALSE +chrUn_JTFH01001188v1_decoy 1129 FALSE +chrUn_JTFH01001189v1_decoy 1127 FALSE +chrUn_JTFH01001190v1_decoy 1127 FALSE +chrUn_JTFH01001191v1_decoy 1118 FALSE +chrUn_JTFH01001192v1_decoy 1110 FALSE +chrUn_JTFH01001193v1_decoy 1104 FALSE +chrUn_JTFH01001194v1_decoy 1104 FALSE +chrUn_JTFH01001195v1_decoy 1101 FALSE +chrUn_JTFH01001196v1_decoy 1098 FALSE +chrUn_JTFH01001197v1_decoy 1096 FALSE +chrUn_JTFH01001198v1_decoy 1094 FALSE +chrUn_JTFH01001199v1_decoy 1091 FALSE +chrUn_JTFH01001200v1_decoy 1089 FALSE +chrUn_JTFH01001201v1_decoy 1086 FALSE +chrUn_JTFH01001202v1_decoy 1085 FALSE +chrUn_JTFH01001203v1_decoy 1084 FALSE +chrUn_JTFH01001204v1_decoy 1083 FALSE +chrUn_JTFH01001205v1_decoy 1083 FALSE +chrUn_JTFH01001206v1_decoy 1079 FALSE +chrUn_JTFH01001207v1_decoy 1076 FALSE +chrUn_JTFH01001208v1_decoy 1069 FALSE +chrUn_JTFH01001209v1_decoy 1068 FALSE +chrUn_JTFH01001210v1_decoy 1067 FALSE +chrUn_JTFH01001211v1_decoy 1067 FALSE +chrUn_JTFH01001212v1_decoy 1067 FALSE +chrUn_JTFH01001213v1_decoy 1063 FALSE +chrUn_JTFH01001214v1_decoy 1062 FALSE +chrUn_JTFH01001215v1_decoy 1059 FALSE +chrUn_JTFH01001216v1_decoy 1058 FALSE +chrUn_JTFH01001217v1_decoy 1058 FALSE +chrUn_JTFH01001218v1_decoy 1055 FALSE +chrUn_JTFH01001219v1_decoy 1054 FALSE +chrUn_JTFH01001220v1_decoy 1054 FALSE +chrUn_JTFH01001221v1_decoy 1053 FALSE +chrUn_JTFH01001222v1_decoy 1053 FALSE +chrUn_JTFH01001223v1_decoy 1052 FALSE +chrUn_JTFH01001224v1_decoy 1051 FALSE +chrUn_JTFH01001225v1_decoy 1049 FALSE +chrUn_JTFH01001226v1_decoy 1047 FALSE +chrUn_JTFH01001227v1_decoy 1044 FALSE +chrUn_JTFH01001228v1_decoy 1043 FALSE +chrUn_JTFH01001229v1_decoy 1043 FALSE +chrUn_JTFH01001230v1_decoy 1042 FALSE +chrUn_JTFH01001231v1_decoy 1042 FALSE +chrUn_JTFH01001232v1_decoy 1041 FALSE +chrUn_JTFH01001233v1_decoy 1040 FALSE +chrUn_JTFH01001234v1_decoy 1039 FALSE +chrUn_JTFH01001235v1_decoy 1038 FALSE +chrUn_JTFH01001236v1_decoy 1037 FALSE +chrUn_JTFH01001237v1_decoy 1037 FALSE +chrUn_JTFH01001238v1_decoy 1035 FALSE +chrUn_JTFH01001239v1_decoy 1027 FALSE +chrUn_JTFH01001240v1_decoy 1021 FALSE +chrUn_JTFH01001241v1_decoy 1021 FALSE +chrUn_JTFH01001242v1_decoy 1019 FALSE +chrUn_JTFH01001243v1_decoy 1019 FALSE +chrUn_JTFH01001244v1_decoy 1016 FALSE +chrUn_JTFH01001245v1_decoy 1014 FALSE +chrUn_JTFH01001246v1_decoy 1013 FALSE +chrUn_JTFH01001247v1_decoy 1009 FALSE +chrUn_JTFH01001248v1_decoy 1008 FALSE +chrUn_JTFH01001249v1_decoy 1007 FALSE +chrUn_JTFH01001250v1_decoy 1004 FALSE +chrUn_JTFH01001251v1_decoy 1004 FALSE +chrUn_JTFH01001252v1_decoy 1003 FALSE +chrUn_JTFH01001253v1_decoy 1001 FALSE +chrUn_JTFH01001254v1_decoy 1000 FALSE +chrUn_JTFH01001255v1_decoy 1000 FALSE +chrUn_JTFH01001256v1_decoy 1000 FALSE +chrUn_JTFH01001257v1_decoy 17929 FALSE +chrUn_JTFH01001258v1_decoy 9749 FALSE +chrUn_JTFH01001259v1_decoy 8053 FALSE +chrUn_JTFH01001260v1_decoy 7826 FALSE +chrUn_JTFH01001261v1_decoy 7768 FALSE +chrUn_JTFH01001262v1_decoy 5691 FALSE +chrUn_JTFH01001263v1_decoy 5444 FALSE +chrUn_JTFH01001264v1_decoy 5077 FALSE +chrUn_JTFH01001265v1_decoy 4990 FALSE +chrUn_JTFH01001266v1_decoy 4545 FALSE +chrUn_JTFH01001267v1_decoy 4544 FALSE +chrUn_JTFH01001268v1_decoy 4202 FALSE +chrUn_JTFH01001269v1_decoy 4195 FALSE +chrUn_JTFH01001270v1_decoy 3807 FALSE +chrUn_JTFH01001271v1_decoy 3741 FALSE +chrUn_JTFH01001272v1_decoy 3699 FALSE +chrUn_JTFH01001273v1_decoy 3640 FALSE +chrUn_JTFH01001274v1_decoy 3531 FALSE +chrUn_JTFH01001275v1_decoy 3455 FALSE +chrUn_JTFH01001276v1_decoy 3411 FALSE +chrUn_JTFH01001277v1_decoy 3387 FALSE +chrUn_JTFH01001278v1_decoy 3358 FALSE +chrUn_JTFH01001279v1_decoy 3285 FALSE +chrUn_JTFH01001280v1_decoy 3273 FALSE +chrUn_JTFH01001281v1_decoy 3262 FALSE +chrUn_JTFH01001282v1_decoy 3259 FALSE +chrUn_JTFH01001283v1_decoy 3222 FALSE +chrUn_JTFH01001284v1_decoy 3127 FALSE +chrUn_JTFH01001285v1_decoy 3110 FALSE +chrUn_JTFH01001286v1_decoy 3104 FALSE +chrUn_JTFH01001287v1_decoy 3071 FALSE +chrUn_JTFH01001288v1_decoy 3063 FALSE +chrUn_JTFH01001289v1_decoy 3059 FALSE +chrUn_JTFH01001290v1_decoy 2990 FALSE +chrUn_JTFH01001291v1_decoy 2986 FALSE +chrUn_JTFH01001292v1_decoy 2928 FALSE +chrUn_JTFH01001293v1_decoy 2922 FALSE +chrUn_JTFH01001294v1_decoy 2875 FALSE +chrUn_JTFH01001295v1_decoy 2859 FALSE +chrUn_JTFH01001296v1_decoy 2850 FALSE +chrUn_JTFH01001297v1_decoy 2813 FALSE +chrUn_JTFH01001298v1_decoy 2785 FALSE +chrUn_JTFH01001299v1_decoy 2736 FALSE +chrUn_JTFH01001300v1_decoy 2688 FALSE +chrUn_JTFH01001301v1_decoy 2658 FALSE +chrUn_JTFH01001302v1_decoy 2643 FALSE +chrUn_JTFH01001303v1_decoy 2618 FALSE +chrUn_JTFH01001304v1_decoy 2605 FALSE +chrUn_JTFH01001305v1_decoy 2583 FALSE +chrUn_JTFH01001306v1_decoy 2534 FALSE +chrUn_JTFH01001307v1_decoy 2512 FALSE +chrUn_JTFH01001308v1_decoy 2500 FALSE +chrUn_JTFH01001309v1_decoy 2481 FALSE +chrUn_JTFH01001310v1_decoy 2478 FALSE +chrUn_JTFH01001311v1_decoy 2473 FALSE +chrUn_JTFH01001312v1_decoy 2467 FALSE +chrUn_JTFH01001313v1_decoy 2442 FALSE +chrUn_JTFH01001314v1_decoy 2430 FALSE +chrUn_JTFH01001315v1_decoy 2417 FALSE +chrUn_JTFH01001316v1_decoy 2408 FALSE +chrUn_JTFH01001317v1_decoy 2395 FALSE +chrUn_JTFH01001318v1_decoy 2352 FALSE +chrUn_JTFH01001319v1_decoy 2337 FALSE +chrUn_JTFH01001320v1_decoy 2322 FALSE +chrUn_JTFH01001321v1_decoy 2307 FALSE +chrUn_JTFH01001322v1_decoy 2306 FALSE +chrUn_JTFH01001323v1_decoy 2292 FALSE +chrUn_JTFH01001324v1_decoy 2271 FALSE +chrUn_JTFH01001325v1_decoy 2265 FALSE +chrUn_JTFH01001326v1_decoy 2260 FALSE +chrUn_JTFH01001327v1_decoy 2240 FALSE +chrUn_JTFH01001328v1_decoy 2238 FALSE +chrUn_JTFH01001329v1_decoy 2228 FALSE +chrUn_JTFH01001330v1_decoy 2215 FALSE +chrUn_JTFH01001331v1_decoy 2205 FALSE +chrUn_JTFH01001332v1_decoy 2191 FALSE +chrUn_JTFH01001333v1_decoy 2191 FALSE +chrUn_JTFH01001334v1_decoy 2190 FALSE +chrUn_JTFH01001335v1_decoy 2184 FALSE +chrUn_JTFH01001336v1_decoy 2166 FALSE +chrUn_JTFH01001337v1_decoy 2165 FALSE +chrUn_JTFH01001338v1_decoy 2162 FALSE +chrUn_JTFH01001339v1_decoy 2146 FALSE +chrUn_JTFH01001340v1_decoy 2116 FALSE +chrUn_JTFH01001341v1_decoy 2112 FALSE +chrUn_JTFH01001342v1_decoy 2108 FALSE +chrUn_JTFH01001343v1_decoy 2106 FALSE +chrUn_JTFH01001344v1_decoy 2106 FALSE +chrUn_JTFH01001345v1_decoy 2106 FALSE +chrUn_JTFH01001346v1_decoy 2097 FALSE +chrUn_JTFH01001347v1_decoy 2081 FALSE +chrUn_JTFH01001348v1_decoy 2058 FALSE +chrUn_JTFH01001349v1_decoy 2055 FALSE +chrUn_JTFH01001350v1_decoy 2054 FALSE +chrUn_JTFH01001351v1_decoy 2037 FALSE +chrUn_JTFH01001352v1_decoy 2032 FALSE +chrUn_JTFH01001353v1_decoy 2032 FALSE +chrUn_JTFH01001354v1_decoy 2020 FALSE +chrUn_JTFH01001355v1_decoy 2018 FALSE +chrUn_JTFH01001356v1_decoy 2014 FALSE +chrUn_JTFH01001357v1_decoy 2001 FALSE +chrUn_JTFH01001358v1_decoy 2001 FALSE +chrUn_JTFH01001359v1_decoy 1991 FALSE +chrUn_JTFH01001360v1_decoy 1990 FALSE +chrUn_JTFH01001361v1_decoy 1983 FALSE +chrUn_JTFH01001362v1_decoy 1981 FALSE +chrUn_JTFH01001363v1_decoy 1981 FALSE +chrUn_JTFH01001364v1_decoy 1979 FALSE +chrUn_JTFH01001365v1_decoy 1963 FALSE +chrUn_JTFH01001366v1_decoy 1932 FALSE +chrUn_JTFH01001367v1_decoy 1929 FALSE +chrUn_JTFH01001368v1_decoy 1881 FALSE +chrUn_JTFH01001369v1_decoy 1874 FALSE +chrUn_JTFH01001370v1_decoy 1849 FALSE +chrUn_JTFH01001371v1_decoy 1849 FALSE +chrUn_JTFH01001372v1_decoy 1833 FALSE +chrUn_JTFH01001373v1_decoy 1832 FALSE +chrUn_JTFH01001374v1_decoy 1826 FALSE +chrUn_JTFH01001375v1_decoy 1814 FALSE +chrUn_JTFH01001376v1_decoy 1814 FALSE +chrUn_JTFH01001377v1_decoy 1791 FALSE +chrUn_JTFH01001378v1_decoy 1789 FALSE +chrUn_JTFH01001379v1_decoy 1786 FALSE +chrUn_JTFH01001380v1_decoy 1778 FALSE +chrUn_JTFH01001381v1_decoy 1776 FALSE +chrUn_JTFH01001382v1_decoy 1762 FALSE +chrUn_JTFH01001383v1_decoy 1758 FALSE +chrUn_JTFH01001384v1_decoy 1757 FALSE +chrUn_JTFH01001385v1_decoy 1754 FALSE +chrUn_JTFH01001386v1_decoy 1752 FALSE +chrUn_JTFH01001387v1_decoy 1751 FALSE +chrUn_JTFH01001388v1_decoy 1749 FALSE +chrUn_JTFH01001389v1_decoy 1738 FALSE +chrUn_JTFH01001390v1_decoy 1729 FALSE +chrUn_JTFH01001391v1_decoy 1726 FALSE +chrUn_JTFH01001392v1_decoy 1716 FALSE +chrUn_JTFH01001393v1_decoy 1712 FALSE +chrUn_JTFH01001394v1_decoy 1711 FALSE +chrUn_JTFH01001395v1_decoy 1703 FALSE +chrUn_JTFH01001396v1_decoy 1702 FALSE +chrUn_JTFH01001397v1_decoy 1699 FALSE +chrUn_JTFH01001398v1_decoy 1686 FALSE +chrUn_JTFH01001399v1_decoy 1684 FALSE +chrUn_JTFH01001400v1_decoy 1680 FALSE +chrUn_JTFH01001401v1_decoy 1678 FALSE +chrUn_JTFH01001402v1_decoy 1678 FALSE +chrUn_JTFH01001403v1_decoy 1677 FALSE +chrUn_JTFH01001404v1_decoy 1676 FALSE +chrUn_JTFH01001405v1_decoy 1672 FALSE +chrUn_JTFH01001406v1_decoy 1669 FALSE +chrUn_JTFH01001407v1_decoy 1668 FALSE +chrUn_JTFH01001408v1_decoy 1663 FALSE +chrUn_JTFH01001409v1_decoy 1660 FALSE +chrUn_JTFH01001410v1_decoy 1660 FALSE +chrUn_JTFH01001411v1_decoy 1658 FALSE +chrUn_JTFH01001412v1_decoy 1656 FALSE +chrUn_JTFH01001413v1_decoy 1656 FALSE +chrUn_JTFH01001414v1_decoy 1652 FALSE +chrUn_JTFH01001415v1_decoy 1647 FALSE +chrUn_JTFH01001416v1_decoy 1645 FALSE +chrUn_JTFH01001417v1_decoy 1641 FALSE +chrUn_JTFH01001418v1_decoy 1638 FALSE +chrUn_JTFH01001419v1_decoy 1633 FALSE +chrUn_JTFH01001420v1_decoy 1626 FALSE +chrUn_JTFH01001421v1_decoy 1614 FALSE +chrUn_JTFH01001422v1_decoy 1612 FALSE +chrUn_JTFH01001423v1_decoy 1605 FALSE +chrUn_JTFH01001424v1_decoy 1603 FALSE +chrUn_JTFH01001425v1_decoy 1599 FALSE +chrUn_JTFH01001426v1_decoy 1589 FALSE +chrUn_JTFH01001427v1_decoy 1588 FALSE +chrUn_JTFH01001428v1_decoy 1585 FALSE +chrUn_JTFH01001429v1_decoy 1584 FALSE +chrUn_JTFH01001430v1_decoy 1584 FALSE +chrUn_JTFH01001431v1_decoy 1580 FALSE +chrUn_JTFH01001432v1_decoy 1572 FALSE +chrUn_JTFH01001433v1_decoy 1570 FALSE +chrUn_JTFH01001434v1_decoy 1569 FALSE +chrUn_JTFH01001435v1_decoy 1568 FALSE +chrUn_JTFH01001436v1_decoy 1567 FALSE +chrUn_JTFH01001437v1_decoy 1565 FALSE +chrUn_JTFH01001438v1_decoy 1559 FALSE +chrUn_JTFH01001439v1_decoy 1559 FALSE +chrUn_JTFH01001440v1_decoy 1556 FALSE +chrUn_JTFH01001441v1_decoy 1554 FALSE +chrUn_JTFH01001442v1_decoy 1549 FALSE +chrUn_JTFH01001443v1_decoy 1542 FALSE +chrUn_JTFH01001444v1_decoy 1541 FALSE +chrUn_JTFH01001445v1_decoy 1538 FALSE +chrUn_JTFH01001446v1_decoy 1537 FALSE +chrUn_JTFH01001447v1_decoy 1535 FALSE +chrUn_JTFH01001448v1_decoy 1530 FALSE +chrUn_JTFH01001449v1_decoy 1528 FALSE +chrUn_JTFH01001450v1_decoy 1522 FALSE +chrUn_JTFH01001451v1_decoy 1514 FALSE +chrUn_JTFH01001452v1_decoy 1509 FALSE +chrUn_JTFH01001453v1_decoy 1507 FALSE +chrUn_JTFH01001454v1_decoy 1500 FALSE +chrUn_JTFH01001455v1_decoy 1499 FALSE +chrUn_JTFH01001456v1_decoy 1499 FALSE +chrUn_JTFH01001457v1_decoy 1497 FALSE +chrUn_JTFH01001458v1_decoy 1496 FALSE +chrUn_JTFH01001459v1_decoy 1488 FALSE +chrUn_JTFH01001460v1_decoy 1486 FALSE +chrUn_JTFH01001461v1_decoy 1485 FALSE +chrUn_JTFH01001462v1_decoy 1481 FALSE +chrUn_JTFH01001463v1_decoy 1479 FALSE +chrUn_JTFH01001464v1_decoy 1472 FALSE +chrUn_JTFH01001465v1_decoy 1472 FALSE +chrUn_JTFH01001466v1_decoy 1470 FALSE +chrUn_JTFH01001467v1_decoy 1466 FALSE +chrUn_JTFH01001468v1_decoy 1465 FALSE +chrUn_JTFH01001469v1_decoy 1461 FALSE +chrUn_JTFH01001470v1_decoy 1458 FALSE +chrUn_JTFH01001471v1_decoy 1457 FALSE +chrUn_JTFH01001472v1_decoy 1448 FALSE +chrUn_JTFH01001473v1_decoy 1447 FALSE +chrUn_JTFH01001474v1_decoy 1444 FALSE +chrUn_JTFH01001475v1_decoy 1443 FALSE +chrUn_JTFH01001476v1_decoy 1443 FALSE +chrUn_JTFH01001477v1_decoy 1438 FALSE +chrUn_JTFH01001478v1_decoy 1432 FALSE +chrUn_JTFH01001479v1_decoy 1430 FALSE +chrUn_JTFH01001480v1_decoy 1430 FALSE +chrUn_JTFH01001481v1_decoy 1429 FALSE +chrUn_JTFH01001482v1_decoy 1429 FALSE +chrUn_JTFH01001483v1_decoy 1429 FALSE +chrUn_JTFH01001484v1_decoy 1426 FALSE +chrUn_JTFH01001485v1_decoy 1426 FALSE +chrUn_JTFH01001486v1_decoy 1420 FALSE +chrUn_JTFH01001487v1_decoy 1416 FALSE +chrUn_JTFH01001488v1_decoy 1416 FALSE +chrUn_JTFH01001489v1_decoy 1415 FALSE +chrUn_JTFH01001490v1_decoy 1415 FALSE +chrUn_JTFH01001491v1_decoy 1414 FALSE +chrUn_JTFH01001492v1_decoy 1413 FALSE +chrUn_JTFH01001493v1_decoy 1410 FALSE +chrUn_JTFH01001494v1_decoy 1405 FALSE +chrUn_JTFH01001495v1_decoy 1402 FALSE +chrUn_JTFH01001496v1_decoy 1398 FALSE +chrUn_JTFH01001497v1_decoy 1397 FALSE +chrUn_JTFH01001498v1_decoy 1395 FALSE +chrUn_JTFH01001499v1_decoy 1392 FALSE +chrUn_JTFH01001500v1_decoy 1388 FALSE +chrUn_JTFH01001501v1_decoy 1386 FALSE +chrUn_JTFH01001502v1_decoy 1382 FALSE +chrUn_JTFH01001503v1_decoy 1381 FALSE +chrUn_JTFH01001504v1_decoy 1379 FALSE +chrUn_JTFH01001505v1_decoy 1376 FALSE +chrUn_JTFH01001506v1_decoy 1374 FALSE +chrUn_JTFH01001507v1_decoy 1374 FALSE +chrUn_JTFH01001508v1_decoy 1373 FALSE +chrUn_JTFH01001509v1_decoy 1373 FALSE +chrUn_JTFH01001510v1_decoy 1372 FALSE +chrUn_JTFH01001511v1_decoy 1370 FALSE +chrUn_JTFH01001512v1_decoy 1367 FALSE +chrUn_JTFH01001513v1_decoy 1365 FALSE +chrUn_JTFH01001514v1_decoy 1364 FALSE +chrUn_JTFH01001515v1_decoy 1361 FALSE +chrUn_JTFH01001516v1_decoy 1361 FALSE +chrUn_JTFH01001517v1_decoy 1355 FALSE +chrUn_JTFH01001518v1_decoy 1355 FALSE +chrUn_JTFH01001519v1_decoy 1354 FALSE +chrUn_JTFH01001520v1_decoy 1353 FALSE +chrUn_JTFH01001521v1_decoy 1349 FALSE +chrUn_JTFH01001522v1_decoy 1345 FALSE +chrUn_JTFH01001523v1_decoy 1344 FALSE +chrUn_JTFH01001524v1_decoy 1343 FALSE +chrUn_JTFH01001525v1_decoy 1338 FALSE +chrUn_JTFH01001526v1_decoy 1338 FALSE +chrUn_JTFH01001527v1_decoy 1338 FALSE +chrUn_JTFH01001528v1_decoy 1336 FALSE +chrUn_JTFH01001529v1_decoy 1333 FALSE +chrUn_JTFH01001530v1_decoy 1333 FALSE +chrUn_JTFH01001531v1_decoy 1332 FALSE +chrUn_JTFH01001532v1_decoy 1324 FALSE +chrUn_JTFH01001533v1_decoy 1323 FALSE +chrUn_JTFH01001534v1_decoy 1323 FALSE +chrUn_JTFH01001535v1_decoy 1320 FALSE +chrUn_JTFH01001536v1_decoy 1320 FALSE +chrUn_JTFH01001537v1_decoy 1317 FALSE +chrUn_JTFH01001538v1_decoy 1316 FALSE +chrUn_JTFH01001539v1_decoy 1304 FALSE +chrUn_JTFH01001540v1_decoy 1304 FALSE 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+HLA-DRB1*16:02:01 11005 TRUE +phix 5386 FALSE diff --git a/Makefile b/Makefile new file mode 100644 index 0000000..20ebdce --- /dev/null +++ b/Makefile @@ -0,0 +1,104 @@ +# Compiler +CXX = x86_64-conda_cos6-linux-gnu-g++ + +INCLUDE_DIR = ./include +BUILD_DIR = ./build +SRC_DIR = ./src + + +INCLUDE_FLAGS= -Iinclude -I$$CONDA_PREFIX/include +LIBDIR_FLAGS = -L$$CONDA_PREFIX/lib + +# Compiler flags +CXXFLAGS = -O3 -Wall -Wextra -static -static-libgcc -static-libstdc++ -flto -c -fmessage-length=0 -Wno-attributes +ifeq ($(STATIC), "true") + CXXFLAGS := -static -static-libgcc -static-libstdc++ $(CXXFLAGS) +endif +CXXFLAGS := $(LIBDIR_FLAGS) -std=c++17 $(INCLUDE_FLAGS) $(CXXFLAGS) + +# Source files +SOURCES = $(wildcard src/*.cpp) + +# Object files should have .o instead of .cpp. +OBJECTS = $(SOURCES:.cpp=.o) + +# Binaries +BINARIES = sophia sophiaAnnotate sophiaMref + +# Default rule +all: $(BINARIES) + +$(BUILD_DIR): + mkdir -p $@ + +# Rule for object files +$(BUILD_DIR)/%.o: %.cpp | $(BUILD_DIR) + $(CXX) $(INCLUDE_FLAGS) $(LIBDIR_FLAGS) $(CXXFLAGS) -c $< -o $@ + +download_strtk: include/strtk.hpp + wget -c https://github.com/ArashPartow/strtk/raw/master/strtk.hpp -O include/strtk.hpp + +vpath %.h includes +vpath %.cpp src + +# Rule for sophia +sophia: $(BUILD_DIR)/Alignment.o \ + $(BUILD_DIR)/Breakpoint.o \ + $(BUILD_DIR)/ChosenBp.o \ + $(BUILD_DIR)/ChrConverter.o \ + $(BUILD_DIR)/Hg37ChrConverter.o \ + $(BUILD_DIR)/Hg38ChrConverter.o \ + $(BUILD_DIR)/SamSegmentMapper.o \ + $(BUILD_DIR)/Sdust.o \ + $(BUILD_DIR)/SuppAlignment.o \ + $(BUILD_DIR)/HelperFunctions.o \ + $(BUILD_DIR)/GlobalAppConfig.o \ + $(BUILD_DIR)/sophia.o \ + download_strtk + $(CXX) $(LIBDIR_FLAGS) -lboost_program_options -o $@ $^ + +# Rule for sophiaAnnotate +sophiaAnnotate: $(BUILD_DIR)/AnnotationProcessor.o \ + $(BUILD_DIR)/Breakpoint.o \ + $(BUILD_DIR)/BreakpointReduced.o \ + $(BUILD_DIR)/ChrConverter.o \ + $(BUILD_DIR)/Hg37ChrConverter.o \ + $(BUILD_DIR)/Hg38ChrConverter.o \ + $(BUILD_DIR)/DeFuzzier.o \ + $(BUILD_DIR)/GermlineMatch.o \ + $(BUILD_DIR)/MrefEntry.o \ + $(BUILD_DIR)/MrefEntryAnno.o \ + $(BUILD_DIR)/MrefMatch.o \ + $(BUILD_DIR)/SuppAlignment.o \ + $(BUILD_DIR)/SuppAlignmentAnno.o \ + $(BUILD_DIR)/SvEvent.o \ + $(BUILD_DIR)/HelperFunctions.o \ + $(BUILD_DIR)/GlobalAppConfig.o \ + $(BUILD_DIR)/sophiaAnnotate.o \ + download_strtk + $(CXX) $(LIBDIR_FLAGS) -lz -lboost_system -lboost_iostreams $(CXXFLAGS) -o $@ $^ + +# Rule for sophiaMref +sophiaMref: $(BUILD_DIR)/GlobalAppConfig.o \ + $(BUILD_DIR)/ChrConverter.o \ + $(BUILD_DIR)/Hg37ChrConverter.o \ + $(BUILD_DIR)/Hg38ChrConverter.o \ + $(BUILD_DIR)/HelperFunctions.o \ + $(BUILD_DIR)/SuppAlignment.o \ + $(BUILD_DIR)/SuppAlignmentAnno.o \ + $(BUILD_DIR)/MrefEntry.o \ + $(BUILD_DIR)/MrefEntryAnno.o \ + $(BUILD_DIR)/MrefMatch.o \ + $(BUILD_DIR)/MasterRefProcessor.o \ + $(BUILD_DIR)/Breakpoint.o \ + $(BUILD_DIR)/BreakpointReduced.o \ + $(BUILD_DIR)/GermlineMatch.o \ + $(BUILD_DIR)/DeFuzzier.o \ + $(BUILD_DIR)/sophiaMref.o \ + download_strtk + $(CXX) $(LIBDIR_FLAGS) -lz -lboost_system -lboost_iostreams -lboost_program_options $(CXXFLAGS) -o $@ $^ + +# Rule for clean +.PHONY: clean +clean: + rm -f $(OBJECTS) $(BINARIES) diff --git a/README.md b/README.md index 61ec2ce..3e36450 100644 --- a/README.md +++ b/README.md @@ -25,40 +25,47 @@ You can cite Sophia as follows: Umut Toprak (2019). DOI 10.11588/heidok.000274296 +### Tools + +* `sophia` - The main tool for SV calling. It takes a BAM file as input and outputs a list of SVs in mref format. +* `sophiaAnnotate` - Tool for annotating SVs with gene information. It reads in an mref file created by `sophiaMref` and annotates the SVs in the input file with gene information. +* `sophiaMref` - The `sophiaMref` tool processes a list of gzipped control bed files and generates a reference that can be used by `sophiaAnnotate` for annotating structural variants with gene information. + +For instructions on commandline parameters, invoke the tool with `--help`. + ## Runtime Dependencies -The only dependency is Boost 1.70.0 (currently). E.g. you can do +The only dependency is Boost 1.82.0 (currently). E.g. you can do ```bash -conda create -n sophia boost=1.70.0 +conda create -n sophia boost=1.82.0 ``` ## Building -### Build-time Dependencies - -* g++ >= 7 -* Boost 1.70.0 - ### Dynamic Build With Conda you can do ```bash -conda create -n sophia gxx_linux-64=8 boost=1.70.0 +conda create -n sophia gxx_linux-64=8 boost=1.82.0 ``` -to create an environment to build the `sophia` and `sophiaAnnotate` binaries. +to create an environment to build the SOPHIA binaries binaries. To build you need to do ```bash source activate sophia -cd Release_sophia -build-sophia.sh + +cd Release_sophiaMref +./build-sophiaMref.sh + +cd ../Release_sophia +./build-sophia.sh cd ../Release_sophiaAnnotate -build-sophiaAnnotate.sh +./build-sophiaAnnotate.sh ``` Note that the build-scripts are for when you manage your dependencies with Conda. diff --git a/include/ChrConverter.h b/include/ChrConverter.h index a3cc126..d96fb4b 100644 --- a/include/ChrConverter.h +++ b/include/ChrConverter.h @@ -26,51 +26,52 @@ namespace sophia { - using namespace std; + // These two are only to make the code clearer, but are not type checked. There are no opaque + // or strongly type-checked typedefs in C++17. + typedef size_t ChrIndex; + typedef size_t CompressedMrefIndex; - /** ChrConverter contains information the names of chromosomes in an assembly. */ - class ChrConverter { - protected: - - /** The constructor should be used to initialize the fields from subclasses. It does - additional checks of the dimensions of the input vectors. */ - ChrConverter(const vector& indexToChr, - const vector& indexToChrCompressedMref, - const vector& chrSizesCompressedMref, - const vector& indexConverter); + /** ChrConverter manages information on chromosomes names, sizes, and index positions in + data arrays. + This probably needs a redesign. The current situation is just an intermediate step away + from the former completely procedural implementation that heavily leaked implementation + details into the calling code and was highly tuned but very inflexible. */ + class ChrConverter { public: virtual ~ChrConverter(); /** The name of the assembly. */ - static const string assembly_name; + static const std::string assemlyName; - /** Mapping indices to chromosome names. */ - const vector indexToChr; + /** Number of chromosomes. */ + virtual int nChromosomes() const = 0; - /** Mapping indices to chromosome names for compressed mref files. */ - const vector indexToChrCompressedMref; + /** Number of compressed mref chromosomes. */ + virtual int nChromosomesCompressedMref() const = 0; - /** Chromosome sizes in base pairs. */ - const vector chrSizesCompressedMref; + /** Map an index position to a chromosome name. */ + virtual std::string indexToChrName(ChrIndex index) const = 0; - /** Mapping chromosome names to indices. */ - const vector indexConverter; + /** Map an index position to a chromosome name for compressed mref files. */ + virtual std::string indexToChrNameCompressedMref(CompressedMrefIndex index) const = 0; - /** Parse chromosome index. It takes a position in a character stream, and translates the - following character(s) into index positions (using ChrConverter::indexToChr). */ - virtual int readChromosomeIndex(string::const_iterator startIt, char stopChar) const = 0; + /** Map the compressed mref index to the uncompressed mref index. */ + virtual ChrIndex compressedMrefIndexToIndex(CompressedMrefIndex index) const = 0; - size_t n_chromosomes() { - return indexToChr.size(); - }; + /** Map compressed mref index to chromosome size. */ + virtual int chrSizeCompressedMref(CompressedMrefIndex index) const = 0; - size_t n_chromosomes_compressed_mref() { - return indexToChrCompressedMref.size(); - }; + /** Map a chromosome name to an index position. */ + virtual ChrIndex chrNameToIndex(std::string chrName) const = 0; + /** Parse chromosome index. It takes a position in a character stream, and translates the + following character(s) into index positions (using ChrConverter::indexToChr). + If the name cannot be parsed, throws a domain_error exception. */ + virtual ChrIndex parseChrAndReturnIndex(std::string::const_iterator startIt, + char stopChar) const = 0; }; diff --git a/include/GlobalAppConfig.h b/include/GlobalAppConfig.h index 2fa80c2..dd0bb3e 100644 --- a/include/GlobalAppConfig.h +++ b/include/GlobalAppConfig.h @@ -38,12 +38,12 @@ namespace sophia { protected: - GlobalAppConfig(unique_ptr chrConverter); + GlobalAppConfig(std::unique_ptr chrConverter); ~GlobalAppConfig(); /** The chromosome converter. */ - const unique_ptr chrConverter; + const std::unique_ptr chrConverter; public: @@ -56,7 +56,7 @@ namespace sophia { void operator=(const GlobalAppConfig &) = delete; /** Factory method. */ - static GlobalAppConfig &init(unique_ptr chrConverter); + static GlobalAppConfig &init(std::unique_ptr chrConverter); /** Getter. */ static const GlobalAppConfig &getInstance(); diff --git a/include/Hg37ChrConverter.h b/include/Hg37ChrConverter.h index 31bfa1b..733a2dc 100644 --- a/include/Hg37ChrConverter.h +++ b/include/Hg37ChrConverter.h @@ -26,16 +26,59 @@ namespace sophia { - using namespace std; - + /** Hard-coded chromosome converter for hg37. This tries to encapsulate the implementation + details of the original version. */ class Hg37ChrConverter: public ChrConverter { + protected: + + /** The constructor does additional checks of the dimensions of the input vectors. */ + Hg37ChrConverter(const std::vector& indexToChr, + const std::vector& indexToChrCompressedMref, + const std::vector& chrSizesCompressedMref, + const std::vector& indexConverter); + + /** Mapping indices to chromosome names. */ + const std::vector indexToChr; + + /** Mapping indices to chromosome names for compressed mref indices. */ + const std::vector indexToChrCompressedMref; + + /** Chromosome sizes in base pairs, only for compressed mref chromosomes. */ + const std::vector chrSizesCompressedMref; + + /** Mapping compressed mref indices names to indices. */ + const std::vector indexConverter; + public: - static const string assembly_name; + static const std::string assemblyName; Hg37ChrConverter(); - int readChromosomeIndex(string::const_iterator startIt, char stopChar) const; + /** Return the number of chromosomes. */ + int nChromosomes() const; + + /** Number of compressed mref chromosomes. */ + int nChromosomesCompressedMref() const; + + /** Map an index position to a chromosome name. */ + std::string indexToChrName(ChrIndex index) const; + + /** Map an index position to a chromosome name for compressed mref files. */ + std::string indexToChrNameCompressedMref(CompressedMrefIndex index) const; + + /** Map the compressed mref index to the uncompressed mref index. */ + ChrIndex compressedMrefIndexToIndex(CompressedMrefIndex index) const; + + /** Map compressed mref index to chromosome size. */ + int chrSizeCompressedMref(CompressedMrefIndex index) const; + + /** Map a chromosome name to an index position for compressed mref files. */ + CompressedMrefIndex chrNameToIndexCompressedMref(std::string chrName) const; + + /** Parse chromosome name given a iterator (start) and termination character. + Validate against the pre-declared chromosome names. */ + ChrIndex parseChrAndReturnIndex(std::string::const_iterator startIt, char stopChar) const; }; diff --git a/include/Hg38ChrConverter.h b/include/Hg38ChrConverter.h index 53db591..45a7e8e 100644 --- a/include/Hg38ChrConverter.h +++ b/include/Hg38ChrConverter.h @@ -22,20 +22,78 @@ #include "ChrConverter.h" #include #include +#include namespace sophia { - using namespace std; + using namespace boost::unordered; class Hg38ChrConverter: public ChrConverter { + + protected: + + /* This makes a search structure to map chromosome names to index positions in an array. + This is useful mostly during the initial parsing of chromosome names from the input. + Afterwards, we continue working with the positions. */ + unordered_map::size_type> makeChrToIndex( + const std::vector& chromosomes + ) const; + + /** Given the index-maps for all chromosomes, and the selected "compressed mref" chromosomes, + create a mapping of mref indices to all chromosome indices. */ + std::vector makeCompressedMrefIndexToIndexConverter( + const unordered_map &allChromosomeLookup, + const unordered_map &compressedMrefChromosomeLookup + ) const; + + const unordered_map allChromosomeLookup; + + const unordered_map compressedMrefChromosomeLookup; + + const std::vector allChromosomeLengths; + + const std::vector compressedMrefIndexToIndexLookup; + const std::vector compressedMrefChromosomes; + + Hg38ChrConverter(const std::vector &allChromosomes, + const std::vector &allChromosomeLengths, + const std::vector &compressedMrefChromosomes); + public: - static const string assembly_name; + static const std::string assemblyName; + /** This default constructor only makes sense, as long as the hg38 chromosome names are + hard-coded. */ Hg38ChrConverter(); - int readChromosomeIndex(string::const_iterator startIt, char stopChar) const; + /** Number of chromosomes. */ + int nChromosomes() const; + + /** Number of compressed mref chromosomes. */ + int nChromosomesCompressedMref() const; + + /** Map an index position to a chromosome name. */ + std::string indexToChrName(ChrIndex index) const; + + /** Map an index position to a chromosome name for compressed mref files. */ + std::string indexToChrNameCompressedMref(CompressedMrefIndex index) const; + + /** Map the compressed mref index to the uncompressed mref index. */ + ChrIndex compressedMrefIndexToIndex(CompressedMrefIndex index) const; + + /** Map compressed mref index to chromosome size. */ + int chrSizeCompressedMref(CompressedMrefIndex index) const; + + /** Map a chromosome name to an index position. */ + ChrIndex chrNameToIndex(std::string chrName) const; + + /** Parse chromosome index. It takes a position in a character stream, and translates the + following character(s) into index positions (using ChrConverter::indexToChr). + If the name cannot be parsed, throws a domain_error exception. */ + ChrIndex parseChrAndReturnIndex(std::string::const_iterator startIt, + char stopChar) const; }; diff --git a/include/MasterRefProcessor.h b/include/MasterRefProcessor.h index 2601905..d98c9c1 100644 --- a/include/MasterRefProcessor.h +++ b/include/MasterRefProcessor.h @@ -41,26 +41,24 @@ namespace sophia { -using namespace std; + class MasterRefProcessor { + public: + MasterRefProcessor(const vector &filesIn, + const string &outputRootName, + const string &version, + const int defaultReadLengthIn); -class MasterRefProcessor { - public: - MasterRefProcessor(const vector &filesIn, - const string &outputRootName, const string &version, - const int defaultReadLengthIn); + ~MasterRefProcessor() = default; - ~MasterRefProcessor() = default; - - private: - unsigned long long processFile(const string &gzPath, short fileIndex); - bool processBp(BreakpointReduced &bp, int chrIndex, short fileIndex); - const int NUMPIDS; - const int DEFAULTREADLENGTH; - unique_ptr mergedBpsOutput; - vector> mrefDb; -}; + private: + unsigned long long processFile(const string &gzPath, short fileIndex); + bool processBp(BreakpointReduced &bp, int chrIndex, short fileIndex); + const int NUMPIDS; + const int DEFAULTREADLENGTH; + unique_ptr mergedBpsOutput; + vector> mrefDb; + }; } // namespace sophia -/* namespace sophiaMref */ #endif /* MASTERREFPROCESSOR_H_ */ diff --git a/sophiaMref/makefile b/sophiaMref/makefile deleted file mode 100644 index fd0bb29..0000000 --- a/sophiaMref/makefile +++ /dev/null @@ -1,58 +0,0 @@ -################################################################################ -# Automatically-generated file. Do not edit! -################################################################################ - --include ../makefile.init - -RM := rm -rf - -# All of the sources participating in the build are defined here --include sources.mk --include src/subdir.mk --include subdir.mk --include objects.mk - -ifneq ($(MAKECMDGOALS),clean) -ifneq ($(strip $(CC_DEPS)),) --include $(CC_DEPS) -endif -ifneq ($(strip $(C++_DEPS)),) --include $(C++_DEPS) -endif -ifneq ($(strip $(C_UPPER_DEPS)),) --include $(C_UPPER_DEPS) -endif -ifneq ($(strip $(CXX_DEPS)),) --include $(CXX_DEPS) -endif -ifneq ($(strip $(CPP_DEPS)),) --include $(CPP_DEPS) -endif -ifneq ($(strip $(C_DEPS)),) --include $(C_DEPS) -endif -endif - --include ../makefile.defs - -# Add inputs and outputs from these tool invocations to the build variables - -# All Target -all: sophiaMref - -# Tool invocations -sophiaMref: $(OBJS) $(USER_OBJS) - @echo 'Building target: $@' - @echo 'Invoking: GCC C++ Linker' - g++ -static -static-libgcc -static-libstdc++ -flto -o "sophiaMref" $(OBJS) $(USER_OBJS) $(LIBS) - @echo 'Finished building target: $@' - @echo ' ' - -# Other Targets -clean: - -$(RM) $(CC_DEPS)$(C++_DEPS)$(EXECUTABLES)$(C_UPPER_DEPS)$(CXX_DEPS)$(OBJS)$(CPP_DEPS)$(C_DEPS) sophiaMref - -@echo ' ' - -.PHONY: all clean dependents - --include ../makefile.targets diff --git a/sophiaMref/src/Alignment.d b/sophiaMref/src/Alignment.d deleted file mode 100644 index 25c1c20..0000000 --- a/sophiaMref/src/Alignment.d +++ /dev/null @@ -1,28 +0,0 @@ -src/Alignment.o: ../src/Alignment.cpp \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/Alignment.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/OverhangRange.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/CigarChunk.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/SuppAlignment.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/ChosenBp.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/CoverageAtBase.h \ - 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/home/umuttoprak/cppProjectsCevelop/sophia/include/ChrConverter.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/Breakpoint.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/DeFuzzier.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/MrefEntry.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/BreakpointReduced.h \ - /home/umuttoprak/cppProjectsCevelop/sophia/include/SuppAlignment.h - -/home/umuttoprak/cppProjectsCevelop/sophia/include/AnnotationProcessor.h: - -/home/umuttoprak/cppProjectsCevelop/sophia/include/BreakpointReduced.h: - -/home/umuttoprak/cppProjectsCevelop/sophia/include/SuppAlignmentAnno.h: - -/home/umuttoprak/cppProjectsCevelop/sophia/include/CigarChunk.h: - -/home/umuttoprak/cppProjectsCevelop/sophia/include/SuppAlignment.h: - -/home/umuttoprak/cppProjectsCevelop/sophia/include/Breakpoint.h: - -/home/umuttoprak/cppProjectsCevelop/sophia/include/Alignment.h: - -/home/umuttoprak/cppProjectsCevelop/sophia/include/OverhangRange.h: - 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