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hisat2-build failed for Segmentation fault #427

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yqlan opened this issue Apr 1, 2024 · 0 comments
Open

hisat2-build failed for Segmentation fault #427

yqlan opened this issue Apr 1, 2024 · 0 comments

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@yqlan
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yqlan commented Apr 1, 2024

I run hisat2-build for a yeast genome using the following command line:

hisat2-build $ref_dir/sacCer3.genome.fa ./ref_idx/sacCer3.genome

it failed with the following message:
"Settings:
Output files: "./ref_idx/sacCer3.genome..ht2"
Line rate: 6 (line is 64 bytes)
Lines per side: 1 (side is 64 bytes)
Offset rate: 4 (one in 16)
FTable chars: 10
Strings: unpacked
Local offset rate: 3 (one in 8)
Local fTable chars: 6
Local sequence length: 57344
Local sequence overlap between two consecutive indexes: 1024
Endianness: little
Actual local endianness: little
Sanity checking: disabled
Assertions: disabled
Random seed: 0
Sizeofs: void
:8, int:4, long:8, size_t:8
Input files DNA, FASTA:
/project2/araman/yqlan/yeastAdaptation_seqData/yeast_reference_genome-genes/sacCer3_buklRNAseq_ref/sacCer3.genome.fa
Reading reference sizes
Segmentation fault"

the genome size is 12M, and I used 50G memory

I'll appreciate if anyone can help. Thanks.
yq

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