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Hi, we tried BASALT full pipeline and it worked out great for us.
We understand that the reassembled bins are in the Final_binset folder and we want to quantify those bins in each sample.
Accoring to the author of MetaWrap it is recommended to use the refined but not reassembled bins as inputs for quantification. Since it hadn't been mentioned in the documents, would you help us point out where they are?
Yes, the folder of Final_binset containes those bins generated by BASALT. The file of 'OLC_bin_stats_ext.tsv' was generated by checkM, so you do not need to re-do checkm again. You could run this directory by gtdbtk, but please beware the you may need to change the suffix to those, e.g. fna, for fitting gtdbtk.
And, we found several bugs in BASALT V1.0.1, and we just uploaded a new version of BASALT(1.0.2) to github. Please update the BASALT program. Thank you.
Hi, we tried BASALT full pipeline and it worked out great for us.
We understand that the reassembled bins are in the Final_binset folder and we want to quantify those bins in each sample.
Accoring to the author of MetaWrap it is recommended to use the refined but not reassembled bins as inputs for quantification. Since it hadn't been mentioned in the documents, would you help us point out where they are?
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Yes, the folder of Final_binset containes those bins generated by BASALT. The file of 'OLC_bin_stats_ext.tsv' was generated by checkM, so you do not need to re-do checkm again. You could run this directory by gtdbtk, but please beware the you may need to change the suffix to those, e.g. fna, for fitting gtdbtk.
And, we found several bugs in BASALT V1.0.1, and we just uploaded a new version of BASALT(1.0.2) to github. Please update the BASALT program. Thank you.
Originally posted by @EMBL-PKU in #38 (comment)
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