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Whenever I run NINJA with the -F option, it fails with the following output. All other params work fine.
(base) @MAC204241 bin $ python ninja.py -i ~/Desktop/reads/forward.fasta,/Users/massoudmaher/Desktop/reads/reverse.fasta -o ~/Desktop/reads_ninja_F -F NINJA-OPS v1.5.1 NINJA-OPS database directory is /Users/massoudmaher/Documents/Code/NINJA-OPS/databases/greengenes97 Running NINJA-OPS filter... "/Users/massoudmaher/Documents/Code/NINJA-OPS/bin/ninja_filter_mac" "/Users/massoudmaher/Desktop/reads/forward.fasta" PE "/Users/massoudmaher/Desktop/reads/reverse.fasta" "/Users/massoudmaher/Desktop/reads_ninja_F/ninja" D 1 LOG Using paired-end reads /Users/massoudmaher/Desktop/reads/reverse.fasta Log writing enabled. Number of sequences: 60522 Total reads considered: 60522 Reads sorted. 60522 Samples found. ************************************* * WARNING!! WARNING!! WARNING!! * * No. of samples = no. of reads * * Casting # of samples to 1. * ************************************* Number of reads rejected = 0, committed = 60522 Finished. NINJA-OPS filter time: 0.344496232 Running Bowtie2... bowtie2-align-s --no-head -x /Users/massoudmaher/Documents/Code/NINJA-OPS/databases/greengenes97/greengenes97 -S "/Users/massoudmaher/Desktop/reads_ninja_F/alignments.txt" --np 0 --mp "1,1" --rdg "0,1" --rfg "0,1" --score-min "L,0,-0.030000000000000027" --norc -f -1 "/Users/massoudmaher/Desktop/reads_ninja_F/ninja_filt.fa" -2 "/Users/massoudmaher/Desktop/reads_ninja_F/ninja2_filt.fa" --maxins 1400 --no-mixed --no-discordant -p 8 --very-sensitive Bowtie time: 30.91181909 Running NINJA-OPS compact... "/Users/massoudmaher/Documents/Code/NINJA-OPS/bin/ninja_compact_mac" "/Users/massoudmaher/Desktop/reads_ninja_F/alignments_uncompacted.txt" "/Users/massoudmaher/Documents/Code/NINJA-OPS/databases/greengenes97/greengenes97.tcf" "/Users/massoudmaher/Desktop/reads_ninja_F/alignments.txt" Done. NINJA-OPS compact time: 0.7563568209999971 Running NINJA-OPS parse... "/Users/massoudmaher/Documents/Code/NINJA-OPS/bin/ninja_parse_filtered_mac" "/Users/massoudmaher/Desktop/reads_ninja_F/ninja" "/Users/massoudmaher/Desktop/reads_ninja_F/alignments.txt" "/Users/massoudmaher/Documents/Code/NINJA-OPS/databases/greengenes97/greengenes97.db" "/Users/massoudmaher/Documents/Code/NINJA-OPS/databases/greengenes97/greengenes97.taxonomy" LOG Traceback (most recent call last): File "ninja.py", line 609, in <module> main(p) File "ninja.py", line 587, in main logger.log("NINJA-OPS parse time: " + str(t3.timeit(1)) + "\n") File "/Users/massoudmaher/miniconda3/lib/python3.7/timeit.py", line 176, in timeit timing = self.inner(it, self.timer) File "<timeit-src>", line 6, in inner File "ninja.py", line 585, in <lambda> logger, legacy_table, run_with_shell=run_with_shell, print_only=args['print_only']) File "ninja.py", line 427, in ninja_parse raise ValueError("ERROR: Parsing failed. Exiting.") ValueError: ERROR: Parsing failed. Exiting. (base) @MAC204241 bin $
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Also, when this happens, the following output files are empty: ninja_otumap.txt, ninja_filtered.txt. But the rest of the files have content
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Whenever I run NINJA with the -F option, it fails with the following output. All other params work fine.
The text was updated successfully, but these errors were encountered: