From d652a443ea2eee4b182c91e19a09332f15ad9c18 Mon Sep 17 00:00:00 2001 From: ffangliu Date: Wed, 31 Aug 2022 21:37:53 -0400 Subject: [PATCH] Updated documentation. Added the example of freezing solute in the advance tutorial --- autosolvate/generatetrajs.py | 3 ++- docs/_images/advancedTutorial3_1.png | Bin 0 -> 224824 bytes docs/advancedTutorial.rst | 32 +++++++++++++++++++++++++++ docs/tutorial.rst | 3 +++ 4 files changed, 37 insertions(+), 1 deletion(-) create mode 100644 docs/_images/advancedTutorial3_1.png diff --git a/autosolvate/generatetrajs.py b/autosolvate/generatetrajs.py index 5763797..fc438d2 100644 --- a/autosolvate/generatetrajs.py +++ b/autosolvate/generatetrajs.py @@ -560,7 +560,8 @@ def startmd(argumentList): -r, --srunuse option to run inside a slurm job -x, --pmemduse Speed up MM with pmemd.CUDA instead of sander -d, --dryrun Dry run mode: only generate the commands to run MD programs and save them into a file without executing the command - -h, --help short usage description + -z, --freezesolute Freeze the solute structure throughout all MD steps + -h, --help short usage description Returns ------- diff --git 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zsEk2SxvYwz`mc7ucwDBtYLS0^9`1L4E zkjL+dHLIN??F8wc@>p~vZ8hh-EhGl-RX`!R2P{lZ{4W6_8sIp;5^~uw`KX!R5+P$h z29R@x59wz47KzVCv)hyH2sW(0E;j+}d3y3t^3j-pP>aF!+Og$+nr;CL&s@MwoZ8=x z2U7^;y?njRnv>sSVU&+&k_VE5CgHz0mMmkG?UMx-PV^H)a8=V%Wf`}OSg8_xLITU4 z5`7k~T^Q{a=&;X_?(*y}rPx0>tEhctx=+dt0e_1z3fEUMznIb2T*9J*j2l3Ec9tb@&*gYqjHULxK7ip8F3ZO_&~v6~O06C8DmJ1P z&CA;?QeDP5V04!p#hpckyKl*%1abV#@YsdhyLH}^tRu^faQZ0!B?x!o|uJhzwQY7`N{S} GfBY}y(}@cJ literal 0 HcmV?d00001 diff --git a/docs/advancedTutorial.rst b/docs/advancedTutorial.rst index 0013067..70a24a4 100644 --- a/docs/advancedTutorial.rst +++ b/docs/advancedTutorial.rst @@ -77,3 +77,35 @@ The automated recommendation of solvent-solute closeness allows to generate init >>> autosolvate boxgen -m naphthalene_neutral.xyz -s water -c 0 -u 1 -t automated with the option `-t automated` the closeness will be automatically determined from the solvent identity. + +Advanced Example 3: Freeze the solute molecule structure +-------------------------------------------------------- + +In certain situations, the user may want to add the solvation shell without changing the solute structure. This can be easily done turning on the "freeze solute" option in the command line or in the GUI interface. This will enable the `ibelly` option in Amber, freezing the solute molecule throughout all MD steps. + +.. warning:: + + The option "freeze solute" will overwrite all the QM/MM options. QM/MM steps will be set to 0. QM/MM simulation will not run. The reason is that if solute is requested to be frozen, then there is no reason to calculate the QM gradients to update the solute structure. Running QM/MM will be a waste of time, because the expensive QM gradients will never be used. + + +To freeze the solute from command line, simply add the `-z` or `--freezesolute` option to your command. For example, for the naphthalene in water example shown in :ref:`Tutorial Step 2`, the command to run MD with frozen solute is: + +``autosolvate mdrun -f water_solvated -q 0 -u 1 -t 300 -p 1 -m 10000 -n 10000 -o 100 -s 100 -l 250 -z`` + +Here, although the user specified the number of steps for QM/MM minimization (`-o`), QMMM heat (`-s`) and QMMM equilibration (`-l`), these options will be ignored and set to 0 because the `-z` option is turned on. The above command is equivalent to + +``autosolvate mdrun -f water_solvated -q 0 -u 1 -t 300 -p 1 -m 10000 -n 10000 -o 0 -s 0 -l 0 -z`` + +and + +``autosolvate mdrun -f water_solvated -q 0 -u 1 -t 300 -p 1 -m 10000 -n 10000 -z`` + + +To freeze the solute from the GUI interface, select "Yes" for the radial button of the "Freeze solute structure?" option in the *mdrun* GUI, as shown in the picture below. + +.. image:: _images/advancedTutorial3_1.png + :width: 800 + + + + diff --git a/docs/tutorial.rst b/docs/tutorial.rst index 62f2f82..9021fa0 100644 --- a/docs/tutorial.rst +++ b/docs/tutorial.rst @@ -182,6 +182,9 @@ With these three files, we are ready to proceed to the next step! The semi-empirical charge fitting available through Amber performs well for closed-shell systems. However, it is not sufficient for open-shell systems, which will require the use of quantum chemistry charge fitting methods. The methods currently available are bcc fitting in Amber and RESP in Gaussian. RESP is the default setting. + +.. _tutstep2: + Step 2: MD Simulation ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~