diff --git a/src/prefect/_internal/concurrency/services.py b/src/prefect/_internal/concurrency/services.py
index 1f992763ef83..eabee601948a 100644
--- a/src/prefect/_internal/concurrency/services.py
+++ b/src/prefect/_internal/concurrency/services.py
@@ -32,7 +32,7 @@ def __init__(self, *args: Hashable) -> None:
         self._task: Optional[asyncio.Task[None]] = None
         self._stopped: bool = False
         self._started: bool = False
-        self._key = hash(args)
+        self._key = hash((self.__class__, *args))
         self._lock = threading.Lock()
         self._queue_get_thread = WorkerThread(
             # TODO: This thread should not need to be a daemon but when it is not, it
@@ -256,7 +256,7 @@ def instance(cls, *args: Hashable) -> Self:
         If an instance already exists with the given arguments, it will be returned.
         """
         with cls._instance_lock:
-            key = hash(args)
+            key = hash((cls, *args))
             if key not in cls._instances:
                 cls._instances[key] = cls._new_instance(*args)
 
diff --git a/tests/_internal/concurrency/test_services.py b/tests/_internal/concurrency/test_services.py
index fe41e17820b3..15cea7996acc 100644
--- a/tests/_internal/concurrency/test_services.py
+++ b/tests/_internal/concurrency/test_services.py
@@ -97,6 +97,14 @@ def test_instance_returns_new_instance_with_unique_key():
     assert isinstance(new_instance, MockService)
 
 
+def test_different_subclasses_have_unique_instances():
+    instance = MockService.instance()
+    assert isinstance(instance, MockService)
+    new_instance = MockBatchedService.instance()
+    assert new_instance is not instance
+    assert isinstance(new_instance, MockBatchedService)
+
+
 def test_instance_returns_same_instance_after_error():
     event = threading.Event()