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This repository has been archived by the owner on Mar 12, 2018. It is now read-only.
It would be great if we could add a step at the end of the pipeline to automatically create a SeqMonk project.
Not sure if this will be possible due to requiring a SeqMonk installation with reference genomes. Could make it optional? Or just ignore a failure?
SeqMonk importer docs:
SeqMonk Importer - Creating SeqMonk Projects from the command line
SYNOPSIS
seqmonk [--(un)spliced] [--mapq=20] --genome "Mus musculus/GRCm38" --outfile out.smk *.bam
DESCRIPTION
This script allows you to run seqmonk in a non-interactive mode to read in
a number of BAM or Bismark coverage files and save these into a single project
file which you can then transfer to an interactive server to do further
downstream analysis.
The options for the program as as follows:
--genome The genome to use for the import. This is specified as
species/assembly and must match an existing genome in your
seqmonk genomes folder.
--outfile The name of the file you want to write the project to
--spliced Split spliced reads so you only see the exonic parts. Will
be added by default if any of the first 100,000 reads in
the first imported BAM file have a splice site in them.
Adding this flag overrides the auto-detection.
--unspliced No not split spliced reads even if they are present. This
flag overrides the default auto-detection.
--mapq Value to use as a MAPQ cutoff for imported reads. Defaults
to 20 if any of the first 100,000 reads has a value above 20.
-h --help Print this help file and exit
-m --memory Set the starting memory allocation in megabytes. Defaults
to 1300. Minimum allowed value is 500 and values above
1300 should only be set on systems running a 64-bit JRE
The text was updated successfully, but these errors were encountered:
It would be great if we could add a step at the end of the pipeline to automatically create a SeqMonk project.
Not sure if this will be possible due to requiring a SeqMonk installation with reference genomes. Could make it optional? Or just ignore a failure?
SeqMonk importer docs:
The text was updated successfully, but these errors were encountered: