diff --git a/README.md b/README.md index 86f7415..cd52369 100644 --- a/README.md +++ b/README.md @@ -9,6 +9,7 @@ PALADIN-plugins can be chained in a pipeline configuration using a single or mul - Difference: Analyze relative differences between two PALADIN taxonomy reports. Can also report specific contributing reads for each difference - GO: Perform gene ontology term grouping and abundance reporting - HPC: Distribute PALADIN execution across cluster nodes +- Pathways: Perform metabolic pathway participation reporting - Plotting: Generate plots in PNG format from pipeline generated data (this feature is currently alpha) - Taxonomy: Perform taxonomic grouping and abundance reporting - Uniprot: Download custom UniProt reports for a PALADIN prepared SAM alignment @@ -51,6 +52,16 @@ Group GO terms and write abundances to a file, filtering for a mapping quality o paladin-plugins.py @@go -i input.tsv -q 20 @@write output.txt ``` +Report the metabolic pathway participation across all phyla for all detected pathways +``` +paladin-plugins.py @@pathways -i input.tsv -q 20 -l 2 +``` + +Report the enzyme counts for all firmicutes in the Streptomycin biosynthesis pathway (ec00521) +``` +paladin-plugins.py @@pathways -i input.tsv -q 20 -s Firmicutes -p ec00521 +``` + Group all bacterial species and write abundances to a file, then plot data to a pie chart, filtering for a mapping quality of 30 and limited number of values shown on graph to 10 ```