diff --git a/admin/server-timing.php b/admin/server-timing.php
index aaead0d989..9c2c7b8e58 100644
--- a/admin/server-timing.php
+++ b/admin/server-timing.php
@@ -43,6 +43,34 @@ function perflab_add_server_timing_page() {
  * @since n.e.x.t
  */
 function perflab_load_server_timing_page() {
+	/*
+	 * This settings section technically includes a field, however it is directly rendered as part of the section
+	 * callback due to requiring custom markup.
+	 */
+	add_settings_section(
+		'output-buffering',
+		__( 'Output Buffering', 'performance-lab' ),
+		'perflab_render_server_timing_page_output_buffering_section',
+		PERFLAB_SERVER_TIMING_SCREEN
+	);
+
+	// Minor style tweaks to improve appearance similar to other core settings screen instances.
+	add_action(
+		'admin_print_styles',
+		static function () {
+			?>
+			<style>
+				.wrap p {
+					max-width: 800px;
+				}
+				.wrap .form-table .td-full {
+					padding-top: 0;
+				}
+			</style>
+			<?php
+		}
+	);
+
 	add_settings_section(
 		'benchmarking',
 		__( 'Benchmarking', 'performance-lab' ),
@@ -58,28 +86,31 @@ static function() {
 					),
 					array( 'code' => array() )
 				);
-				?>
-				<br>
-				<?php
+				echo ' ';
 				echo wp_kses(
 					__( 'For any hook name provided, the <strong>cumulative duration between all callbacks</strong> attached to the hook is measured, in milliseconds.', 'performance-lab' ),
 					array( 'strong' => array() )
 				);
-				if ( ! perflab_server_timing_use_output_buffer() ) {
-					?>
-					<br>
+				?>
+			</p>
+			<?php if ( ! perflab_server_timing_use_output_buffer() ) : ?>
+				<p>
 					<?php
 					echo wp_kses(
 						sprintf(
-							/* translators: 1: Server-Timing, 2: template_include */
-							__( 'Since the %1$s header is sent before the template is loaded, only hooks before the %2$s filter can be measured.', 'performance-lab' ),
+							/* translators: 1: Server-Timing, 2: template_include, 3: anchor link */
+							__( 'Since the %1$s header is sent before the template is loaded, only hooks before the %2$s filter can be measured. Enable <a href="%3$s">Output Buffering</a> to measure hooks during template rendering.', 'performance-lab' ),
 							'<code>Server-Timing</code>',
-							'<code>template_include</code>'
+							'<code>template_include</code>',
+							esc_url( '#server_timing_output_buffering' )
 						),
-						array( 'code' => array() )
+						array(
+							'code' => array(),
+							'a'    => array( 'href' => true ),
+						)
 					);
-				}
-				?>
+					?>
+				<?php endif; ?>
 			</p>
 			<?php
 		},
@@ -95,7 +126,7 @@ static function() {
 		'benchmarking_actions',
 		__( 'Actions', 'performance-lab' ),
 		static function() {
-			perflab_render_server_timing_page_field( 'benchmarking_actions' );
+			perflab_render_server_timing_page_hooks_field( 'benchmarking_actions' );
 		},
 		PERFLAB_SERVER_TIMING_SCREEN,
 		'benchmarking',
@@ -105,7 +136,7 @@ static function() {
 		'benchmarking_filters',
 		__( 'Filters', 'performance-lab' ),
 		static function() {
-			perflab_render_server_timing_page_field( 'benchmarking_filters' );
+			perflab_render_server_timing_page_hooks_field( 'benchmarking_filters' );
 		},
 		PERFLAB_SERVER_TIMING_SCREEN,
 		'benchmarking',
@@ -136,13 +167,13 @@ function perflab_render_server_timing_page() {
 }
 
 /**
- * Renders a field for the given Server-Timing option.
+ * Renders a hooks field for the given Server-Timing option.
  *
  * @since n.e.x.t
  *
  * @param string $slug Slug of the field and sub-key in the Server-Timing option.
  */
-function perflab_render_server_timing_page_field( $slug ) {
+function perflab_render_server_timing_page_hooks_field( $slug ) {
 	$options = (array) get_option( PERFLAB_SERVER_TIMING_SETTING, array() );
 
 	// Value for the sub-key is an array of hook names.
@@ -168,3 +199,71 @@ class="large-text code"
 	</p>
 	<?php
 }
+
+/**
+ * Renders the section for enabling output buffering for Server-Timing.
+ *
+ * @since n.e.x.t
+ */
+function perflab_render_server_timing_page_output_buffering_section() {
+	$slug           = 'output_buffering';
+	$field_id       = "server_timing_{$slug}";
+	$field_name     = PERFLAB_SERVER_TIMING_SETTING . '[' . $slug . ']';
+	$description_id = "{$field_id}_description";
+	$has_filter     = has_filter( 'perflab_server_timing_use_output_buffer' );
+	$is_enabled     = perflab_server_timing_use_output_buffer();
+
+	/*
+	 * The hard-coded .form-table output here overall matches the WordPress core markup generated by
+	 * `do_settings_sections()` and `do_settings_fields()`, however since it is impossible to modify the CSS classes on
+	 * the `<td>` elements, it needs to be hard-coded to achieve the same appearance as e.g. the UI control for the
+	 * `uploads_use_yearmonth_folders` option in the _Settings > Media_ screen, which is hard-coded as well.
+	 */
+	?>
+	<table class="form-table" role="presentation">
+		<tr>
+			<td class="td-full">
+				<label for="<?php echo esc_attr( $field_id ); ?>">
+					<input
+						type="checkbox"
+						id="<?php echo esc_attr( $field_id ); ?>"
+						name="<?php echo esc_attr( $field_name ); ?>"
+						aria-describedby="<?php echo esc_attr( $description_id ); ?>"
+						<?php disabled( $has_filter ); ?>
+						<?php checked( $is_enabled ); ?>
+					>
+					<?php esc_html_e( 'Enable output buffering of template rendering', 'performance-lab' ); ?>
+				</label>
+				<p id="<?php echo esc_attr( $description_id ); ?>" class="description">
+					<?php if ( $has_filter ) : ?>
+						<?php if ( $is_enabled ) : ?>
+							<?php
+							echo wp_kses(
+								sprintf(
+									/* translators: %s: perflab_server_timing_use_output_buffer */
+									__( 'Output buffering has been forcibly enabled via the %s filter.', 'performance-lab' ),
+									'<code>perflab_server_timing_use_output_buffer</code>'
+								),
+								array( 'code' => array() )
+							);
+							?>
+						<?php else : ?>
+							<?php
+							echo wp_kses(
+								sprintf(
+									/* translators: %s: perflab_server_timing_use_output_buffer */
+									__( 'Output buffering has been forcibly disabled via the %s filter.', 'performance-lab' ),
+									'<code>perflab_server_timing_use_output_buffer</code>'
+								),
+								array( 'code' => array() )
+							);
+							?>
+						<?php endif; ?>
+					<?php endif; ?>
+					<?php esc_html_e( 'Output buffering is needed to capture metrics after headers have been sent and while the template is being rendered. Note that output buffering may possibly cause an increase in TTFB if the response would be flushed multiple times.', 'performance-lab' ); ?>
+				</p>
+			</td>
+		</tr>
+	</table>
+	<?php
+}
diff --git a/server-timing/class-perflab-server-timing.php b/server-timing/class-perflab-server-timing.php
index 094d57be3a..833e10a400 100644
--- a/server-timing/class-perflab-server-timing.php
+++ b/server-timing/class-perflab-server-timing.php
@@ -195,6 +195,9 @@ static function( $value ) {
 	 * @return bool True if an output buffer should be used, false otherwise.
 	 */
 	public function use_output_buffer() {
+		$options = (array) get_option( PERFLAB_SERVER_TIMING_SETTING, array() );
+		$enabled = ! empty( $options['output_buffering'] );
+
 		/**
 		 * Filters whether an output buffer should be used to be able to gather additional Server-Timing metrics.
 		 *
@@ -206,7 +209,7 @@ public function use_output_buffer() {
 		 *
 		 * @param bool $use_output_buffer Whether to use an output buffer.
 		 */
-		return apply_filters( 'perflab_server_timing_use_output_buffer', false );
+		return (bool) apply_filters( 'perflab_server_timing_use_output_buffer', $enabled );
 	}
 
 	/**
diff --git a/server-timing/load.php b/server-timing/load.php
index 322f1a0dc7..fa332bcd9d 100644
--- a/server-timing/load.php
+++ b/server-timing/load.php
@@ -163,6 +163,7 @@ function perflab_sanitize_server_timing_setting( $value ) {
 	static $allowed_keys = array(
 		'benchmarking_actions' => true,
 		'benchmarking_filters' => true,
+		'output_buffering'     => true,
 	);
 
 	if ( ! is_array( $value ) ) {
@@ -175,7 +176,7 @@ function perflab_sanitize_server_timing_setting( $value ) {
 	 * Ensure that every element is an indexed array of hook names.
 	 * Any duplicates across a group of hooks are removed.
 	 */
-	foreach ( $value as $key => $hooks ) {
+	foreach ( wp_array_slice_assoc( $value, array( 'benchmarking_actions', 'benchmarking_filters' ) ) as $key => $hooks ) {
 		if ( ! is_array( $hooks ) ) {
 			$hooks = explode( "\n", $hooks );
 		}
@@ -203,5 +204,7 @@ static function( $hookname ) {
 		);
 	}
 
+	$value['output_buffering'] = ! empty( $value['output_buffering'] );
+
 	return $value;
 }
diff --git a/tests/admin/server-timing-tests.php b/tests/admin/server-timing-tests.php
index c764f9dda0..0684ff8409 100644
--- a/tests/admin/server-timing-tests.php
+++ b/tests/admin/server-timing-tests.php
@@ -49,7 +49,7 @@ public function test_perflab_load_server_timing_page() {
 		perflab_load_server_timing_page();
 		$this->assertArrayHasKey( PERFLAB_SERVER_TIMING_SCREEN, $wp_settings_sections );
 		$this->assertEqualSets(
-			array( 'benchmarking' ),
+			array( 'output-buffering', 'benchmarking' ),
 			array_keys( $wp_settings_sections[ PERFLAB_SERVER_TIMING_SCREEN ] )
 		);
 		$this->assertEqualSets(
@@ -75,7 +75,7 @@ public function test_perflab_render_server_timing_page_field() {
 		$slug = 'benchmarking_actions';
 
 		ob_start();
-		perflab_render_server_timing_page_field( $slug );
+		perflab_render_server_timing_page_hooks_field( $slug );
 		$output = ob_get_clean();
 
 		$this->assertStringContainsString( '<textarea', $output );
@@ -87,7 +87,7 @@ public function test_perflab_render_server_timing_page_field_empty_option() {
 		delete_option( PERFLAB_SERVER_TIMING_SETTING );
 
 		ob_start();
-		perflab_render_server_timing_page_field( 'benchmarking_actions' );
+		perflab_render_server_timing_page_hooks_field( 'benchmarking_actions' );
 		$output = ob_get_clean();
 
 		$this->assertStringContainsString( '></textarea>', $output );
@@ -100,7 +100,7 @@ public function test_perflab_render_server_timing_page_field_populated_option()
 		);
 
 		ob_start();
-		perflab_render_server_timing_page_field( 'benchmarking_actions' );
+		perflab_render_server_timing_page_hooks_field( 'benchmarking_actions' );
 		$output = ob_get_clean();
 
 		// Array is formatted/imploded as strings, one per line.
diff --git a/tests/server-timing/load-tests.php b/tests/server-timing/load-tests.php
index b695bdc64d..7d6fd3e2c7 100644
--- a/tests/server-timing/load-tests.php
+++ b/tests/server-timing/load-tests.php
@@ -103,39 +103,43 @@ public function data_perflab_sanitize_server_timing_setting() {
 			),
 			'empty list, array'                           => array(
 				array( 'benchmarking_actions' => array() ),
-				array( 'benchmarking_actions' => array() ),
+				array( 'benchmarking_actions' => array(), 'output_buffering' => false ),
 			),
 			'empty list, string'                          => array(
 				array( 'benchmarking_actions' => '' ),
-				array( 'benchmarking_actions' => array() ),
+				array( 'benchmarking_actions' => array(), 'output_buffering' => false ),
 			),
 			'empty list, string with whitespace'          => array(
 				array( 'benchmarking_actions' => ' ' ),
-				array( 'benchmarking_actions' => array() ),
+				array( 'benchmarking_actions' => array(), 'output_buffering' => false ),
 			),
 			'regular list, array'                         => array(
 				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ) ),
-				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ) ),
+				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ), 'output_buffering' => false ),
 			),
 			'regular list, string'                        => array(
 				array( 'benchmarking_actions' => "after_setup_theme\ninit\nwp_loaded" ),
-				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ) ),
+				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ), 'output_buffering' => false ),
 			),
 			'regular list, string with whitespace'        => array(
 				array( 'benchmarking_actions' => "after_setup_  theme \ninit \n\nwp_loaded\n" ),
-				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ) ),
+				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ), 'output_buffering' => false ),
 			),
 			'regular list, array with duplicates'         => array(
 				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded', 'init' ) ),
-				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ) ),
+				array( 'benchmarking_actions' => array( 'after_setup_theme', 'init', 'wp_loaded' ), 'output_buffering' => false ),
 			),
 			'regular list, array with special hook chars' => array(
 				array( 'benchmarking_actions' => array( 'namespace/hookname', 'namespace.hookname' ) ),
-				array( 'benchmarking_actions' => array( 'namespace/hookname', 'namespace.hookname' ) ),
+				array( 'benchmarking_actions' => array( 'namespace/hookname', 'namespace.hookname' ), 'output_buffering' => false ),
+			),
+			'output buffering enabled' => array(
+				array( 'output_buffering' => 'on' ),
+				array( 'output_buffering' => true ),
 			),
 			'regular list, disallowed key'                => array(
 				array( 'not_allowed' => array( 'after_setup_theme', 'init', 'wp_loaded' ) ),
-				array(),
+				array( 'output_buffering' => false ),
 			),
 		);
 	}
diff --git a/tests/server-timing/perflab-server-timing-tests.php b/tests/server-timing/perflab-server-timing-tests.php
index cc9aa57157..360d4547b1 100644
--- a/tests/server-timing/perflab-server-timing-tests.php
+++ b/tests/server-timing/perflab-server-timing-tests.php
@@ -187,10 +187,58 @@ public function data_get_header() {
 		);
 	}
 
-	public function test_use_output_buffer() {
-		$this->assertFalse( $this->server_timing->use_output_buffer() );
+	public function get_data_to_test_use_output_buffer() {
+		$enable_option = static function () {
+			$option = (array) get_option( PERFLAB_SERVER_TIMING_SETTING );
+			$option['output_buffering'] = true;
+			update_option( PERFLAB_SERVER_TIMING_SETTING, $option );
+		};
+		$disable_option = static function () {
+			$option = (array) get_option( PERFLAB_SERVER_TIMING_SETTING );
+			$option['output_buffering'] = false;
+			update_option( PERFLAB_SERVER_TIMING_SETTING, $option );
+		};
 
-		add_filter( 'perflab_server_timing_use_output_buffer', '__return_true' );
-		$this->assertTrue( $this->server_timing->use_output_buffer() );
+		return array(
+			'default' => array(
+				'set_up'   => static function () {},
+				'expected' => false,
+			),
+			'option-enabled' => array(
+				'set_up'   => $enable_option,
+				'expected' => true,
+			),
+			'option-disabled' => array(
+				'set_up'   => $disable_option,
+				'expected' => false,
+			),
+			'filter-enabled' => array(
+				'set_up'   => static function () use ( $disable_option ) {
+					$disable_option();
+					add_filter( 'perflab_server_timing_use_output_buffer', '__return_true' );
+				},
+				'expected' => true,
+			),
+			'filter-disabled' => array(
+				'set_up'   => static function () use ( $enable_option ) {
+					$enable_option();
+					add_filter( 'perflab_server_timing_use_output_buffer', '__return_false' );
+				},
+				'expected' => false,
+			),
+		);
+	}
+
+	/**
+	 * @covers Perflab_Server_Timing::use_output_buffer
+	 *
+	 * @dataProvider get_data_to_test_use_output_buffer
+	 *
+	 * @param callable $set_up   Set up.
+	 * @param bool     $expected Expected value.
+	 */
+	public function test_use_output_buffer( callable $set_up, $expected ) {
+		$set_up();
+		$this->assertSame( $expected, $this->server_timing->use_output_buffer() );
 	}
 }