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back_spliced_junctions.bed #59

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ericbrzoska opened this issue Feb 16, 2022 · 0 comments
Open

back_spliced_junctions.bed #59

ericbrzoska opened this issue Feb 16, 2022 · 0 comments

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@ericbrzoska
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Hi,

my task is to compute the coverage from the tophat2 accepted_hits.bam with the back_spliced_junction.bed, but I don´t know if the results I got from...

samtools depth -H -o Coverage/cov_TT17 -b back_spliced_junction.bed accepted_hits.bam

...are right. Can someone explain me what the columns after the first three [chrom, start, end] ones describe? Or does someone know if they are even used by samtools?
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@ericbrzoska