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It seems that a circRNA had 0 reads yet it was annotated (ENST00000361204). Why? What does it mean?
For ENST00000255784 transcript, it is annotated twice as two different circRNAs. How would I know the weight of this transcript? How sure am I that it would not be a 3rd transcript? Should I use bedfiles? At which step?
After the annotation step, how do you actually cross the information between the circRNAs that were aligned and annotated? How do you extract the exact coordinates from the reads per gene file?
Thanks a lot for your help.
The text was updated successfully, but these errors were encountered:
For instance, let's say that my reads per gene contains the following information for these two transcripts (Reads per gene file obtained with STAR):
So 0 reads per ENST00000361204 transcript and 842 reads per ENST00000255784 transcript.
And the file generated during annotation step contains lines like the following:
Questions for you:
Thanks a lot for your help.
The text was updated successfully, but these errors were encountered: