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failure to demultiplex manifest #39
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Hey there, Are you still having this problem? Would be curious to see your manifest file if so. Thanks, |
Hi, did you somehow solve the problem with the demultiplexing process? |
Having the same issue. Anybody got any hints? |
Hey Zethson, |
Running
test_manifest.yaml
as well as thefull_test.sh
on the platform performs completely fine. My manifest file loads successfully (contains only a control and a single sample) and the demultiplexing process continues without error, however when UMI tagging begins I receive:[10/11 05:16:15PM][ERROR][guideseq] Error umitagging
[10/11 05:16:15PM][ERROR][guideseq] Traceback (most recent call last): File "guideseq/guideseq.py", line 150, in umitag os.path.join(self.output_folder, 'umitagged')) File "/projects/guideseq/guideseq/umi/umitag.py", line 56, in umitag for r1,r2,i1,i2 in itertools.izip(fq(read1), fq(read2), fq(index1), fq(index2)): File "/projects/guideseq/guideseq/umi/umitag.py", line 26, in fq fastq = open(file, 'r')
IOError: [Errno 2] No such file or directory: './run/output/demultiplexed/control.r1.fastq'
Upon inspection of the demultiplexed output directory, there are almost 1500 files with names such as "AAGAGAAATCCTCT.i1.fastq" and the corresponding i2, r1, r2. It seems the true error is occurring during the demultiplexing step. Any idea what is going on here?
Also, after the demultiplexing step the console outputs
Wrote FASTQs for the 357 sample barcodes out of 128400 with at least 1000 reads.
However my manifest only contains 2 samples, including the control.The text was updated successfully, but these errors were encountered: