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unsupported operand type(s) #55
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I also have the same issue as you but I follow all the previous steps, no skip of the UMI step. |
Are you able to a) check how many reads are in the input SAM file, and b) post a few of the lines from the SAM file? |
Using the following command For and example please see the file below |
Thank you for providing the information. To help debug further, could you please provide your outputs from the (1) UMItag step and (2) the align step? Thank you. |
I did not use the UMItag step and the align step. The raw data I used does not have any UMI data. The github stated the program can be run in individual parts so I only used already aligned data with the guideseq program for identifying off-target sites. |
Hi, I had the same problem. I didn't use UMI. So I just started from "align".
Do you solve this problem? |
Same problem here, thanks |
Hi, I had the same problem. I didn't use UMI. Do you solve this problem? |
HI Anna,
sorry for not being very helpful but I was not able to find an
answer/solution for that.
I got frustrated because it seems to be a common problem if you are not
using UMIs but nobody could provide a clear solution.
All the best,
Laura
El lun, 26 sept 2022 a las 13:57, Anna-xu408 ***@***.***>)
escribió:
… Hi, I had the same problem. I didn't use UMI. Do you solve this problem?
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Laura Hernández Hernández
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I am having this problem using the SAM file to identify the off-target
python ~/bin/guideseq-1.0.2/guideseq/guideseq.py identify --aligned ~/Desktop/WORKS/CODE/download/AA_1.sam --target_sequence ctgcgcgctcgctcgctcactgaggccgcccgg --description ABM --genome ~/Desktop/WORKS/CODE/download/ucsc_mm10.fa --outfolder ~/Desktop/WORKS/Analisi/Auricchio/Manel/identify/
[07/08 09:20:09AM][INFO][guideseq] Identifying offtarget sites...
[07/08 09:20:09AM][INFO][identifyOfftargetSites] Processing SAM file /home/francesco/Desktop/WORKS/CODE/download/AA_1.sam
[07/08 09:20:09AM][ERROR][guideseq] Error identifying offtarget sites.
[07/08 09:20:09AM][ERROR][guideseq] Traceback (most recent call last):
File "/home/francesco/bin/guideseq-1.0.2/guideseq/guideseq.py", line 224, in identifyOfftargetSites
identifyOfftargetSites.analyze(samfile, self.reference_genome, self.identified[sample], annotations)
File "/home/francesco/bin/guideseq-1.0.2/guideseq/identifyOfftargetSites.py", line 217, in analyze
chromosome_position.addPositionBarcode(chromosome, read_position, strand, barcode, primer, count)
File "/home/francesco/bin/guideseq-1.0.2/guideseq/identifyOfftargetSites.py", line 66, in addPositionBarcode
self.chromosome_barcode_dict[chromosome][position][strand][barcode] += count
TypeError: unsupported operand type(s) for +=: 'int' and 'NoneType'
Do you know what can be the problem?
I did not use the UMI and started from "align" step
Thanks a lot for youtr time
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