From 3ec2536b4447eaa4e2b48b33822cd39d91eeb5bc Mon Sep 17 00:00:00 2001 From: Alex Bartlett <74612800+abartlett004@users.noreply.github.com> Date: Tue, 1 Oct 2024 14:15:31 -0400 Subject: [PATCH] Update README.md --- inst/templates/rnaseq/README.md | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/inst/templates/rnaseq/README.md b/inst/templates/rnaseq/README.md index 6de2117..e6e6cbc 100644 --- a/inst/templates/rnaseq/README.md +++ b/inst/templates/rnaseq/README.md @@ -5,8 +5,8 @@ Make sure there is a project name for this. ## Run data with nf-core rnaseq - Make sure you have access to our [Seqera WorkSpace](https://cloud.seqera.io/orgs/HBC/workspaces/core_production/launchpad) -- Transfer data to HCBC S3: Ask Alex/Lorena. Files will be at our S3 bucket `input/rawdata` folder -- Prepare the CSV file according this [instructions](https://nf-co.re/rnaseq/3.14.0/docs/usage#multiple-runs-of-the-same-sample). File should look like this: +- Transfer data to HCBC S3: Ask Alex/Lorena. Files will be at our S3 bucket `input` folder +- Prepare the CSV file according these [instructions](https://nf-co.re/rnaseq/3.14.0/docs/usage#multiple-runs-of-the-same-sample). File should look like this: ```csv sample,fastq_1,fastq_2,strandedness @@ -22,11 +22,11 @@ You can add more columns to this file with more metadata, and use this file as t - Upload file to our `Datasets` in Seqera using the name of the project but starting with `rnaseq-pi_lastname-hbc_code` - Go to `Launchpad`, select `nf-core_rnaseq` pipeline, and select the previous created `Datasets` in the `input` parameter after clicking in `Browser` - Select an output directory with the same name used for the `Dataset` inside the `results` folder in S3 -- When pipeline is down, data will be copied to our on-premise HPC in the scratch system under `scratch/groups/hsph/hbc/bcbio/` folder +- When pipeline is done, data will be copied to our on-premise HPC in the scratch system under `scratch/groups/hsph/hbc/bcbio/` folder ## Downstream analysis -Please, modify `information.R` with the right information. You can use this file with any other Rmd to include the project/analysis information. +Modify `information.R` with the right information. You can use this file with any other Rmd to include the project/analysis information. ### QC @@ -37,7 +37,7 @@ Read instruction in the R and Rmd scripts to render it. ### DE -`DE/DEG.Rmd` is a template for two groups comparison. `params_de.R` has the information of the input files to load. You can point to `bcbio` or `nf-core/rnaseq` output files. +`DE/DEG.Rmd` is a template for comparison between two groups. `params_de.R` has the information for the input files to load. You can point to `bcbio` or `nf-core/rnaseq` output files. On the `YAML` header file of the `Rmd` you can specify some parameters or just set them up in the first chunk of code of the template. This template has examples of: