diff --git a/CHANGELOG.md b/CHANGELOG.md index 2216d131..b9f3cbfa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -18,13 +18,16 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Removed` +- [#238](https://github.com/genomic-medicine-sweden/nallo/pull/238) - Removed the `--extra_gvcfs` parameter + ### `Fixed` ### Parameters -| Old parameter | New parameter | -| ------------- | -------------------------- | -| | `--deepvariant_model_type` | +| Old parameter | New parameter | +| --------------- | -------------------------- | +| | `--deepvariant_model_type` | +| `--extra_gvcfs` | | ## v0.2.0 - [2024-06-26] diff --git a/docs/usage.md b/docs/usage.md index 82b1f4d0..043ac92d 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -133,20 +133,6 @@ HG01123,/path/to/HG01123_sniffles.snf HG01124,/path/to/HG01124_sniffles.snf ``` -- For SNVs - prepare a samplesheet with gVCF files from DeepVariant to supply with `--extra_gvcfs`: - -> [!NOTE] -> These has to have been generated with the same version of reference genome. - -`extra_gvcfs.csv` - -``` -sample,file -HG01123,/path/to/HG01123.g.vcf.gz -HG01124,/path/to/HG01124.g.vcf.gz -HG01125,/path/to/HG01125.g.vcf.gz -``` - - If running without `--skip_call_paralogs`, the reference genome needs to be hg38 - If running without `--skip_mapping_wf`, a VCF of known polymorphic sites (e.g. [sites.hg38.vcg.gz](https://github.com/brentp/somalier/files/3412456/sites.hg38.vcf.gz)) needs to be supplied with `--somalier_sites`, from which sex will be inferred if possible. @@ -263,7 +249,6 @@ Different processes may need extra input files | Parameter | Description | Type | Default | Required | Hidden | | ---------------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | | `dipcall_par` | Provide a bed file of chrX PAR regions for dipcall | `string` | | | | -| `extra_gvcfs` | Extra input files for GLNexus | `string` | | | | | `extra_snfs` | Extra input files for Sniffles | `string` | | | | | `tandem_repeats` | Tandem repeat BED-file for sniffles | `string` | | | | | `trgt_repeats` | BED-file for repeats to be genotyped | `string` | | | | diff --git a/nextflow.config b/nextflow.config index 70e8cdde..b3a66e92 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,6 @@ params { bed = null dipcall_par = null extra_snfs = null - extra_gvcfs = null tandem_repeats = null trgt_repeats = null snp_db = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 999a7e4d..c0faba57 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -392,14 +392,6 @@ "format": "file-path", "exists": true }, - "extra_gvcfs": { - "type": "string", - "description": "Extra input files for GLNexus", - "pattern": "^\\S+\\.csv$", - "format": "file-path", - "schema": "assets/schema_gvcfs.json", - "exists": true - }, "extra_snfs": { "type": "string", "description": "Extra input files for Sniffles", diff --git a/subworkflows/local/short_variant_calling/main.nf b/subworkflows/local/short_variant_calling/main.nf index 1bfe58cd..3bee882e 100644 --- a/subworkflows/local/short_variant_calling/main.nf +++ b/subworkflows/local/short_variant_calling/main.nf @@ -1,7 +1,5 @@ include { DEEPVARIANT } from '../../../modules/nf-core/deepvariant' include { GLNEXUS } from '../../../modules/nf-core/glnexus' -include { BCFTOOLS_VIEW_REGIONS } from '../../../modules/local/bcftools/view_regions' -include { TABIX_TABIX as TABIX_EXTRA_GVCFS } from '../../../modules/nf-core/tabix/tabix/main' include { TABIX_TABIX as TABIX_DV } from '../../../modules/nf-core/tabix/tabix/main' include { TABIX_TABIX as TABIX_DV_VCF } from '../../../modules/nf-core/tabix/tabix/main' include { TABIX_TABIX as TABIX_GLNEXUS } from '../../../modules/nf-core/tabix/tabix/main' @@ -14,7 +12,6 @@ workflow SHORT_VARIANT_CALLING { take: ch_bam_bai_bed // channel: [ val(meta), path(bam), path(bai), path(call_region_bed) ] - ch_extra_gvcfs // channel: [ val(meta), path(gvcfs) ] ch_fasta // channel: [ val(meta), path(fasta) ] ch_fai // channel: [ val(meta), path(fai) ] ch_bed // channel: [ val(meta), path(input_bed) ] @@ -33,17 +30,6 @@ workflow SHORT_VARIANT_CALLING { // Collect GVCFs ch_snp_calls_gvcf = ch_snp_calls_gvcf.mix(DEEPVARIANT.out.gvcf) - // Extra gVCFs - TABIX_EXTRA_GVCFS(ch_extra_gvcfs) - - ch_extra_gvcfs - .join(TABIX_EXTRA_GVCFS.out.tbi) - .groupTuple() - .set{ ch_bcftools_view_regions_in } - - // This cuts all regions in BED file from extra gVCFS, better than nothing - BCFTOOLS_VIEW_REGIONS( ch_bcftools_view_regions_in, ch_bed ) - // DV gVCFs TABIX_DV(ch_snp_calls_gvcf) @@ -52,7 +38,6 @@ workflow SHORT_VARIANT_CALLING { .join(TABIX_DV.out.tbi.groupTuple()) .set{ bcftools_concat_dv_in } - // Concat into one gVCF per sample & sort BCFTOOLS_CONCAT_DV ( bcftools_concat_dv_in ) ch_versions = ch_versions.mix(BCFTOOLS_CONCAT_DV.out.versions) @@ -79,7 +64,6 @@ workflow SHORT_VARIANT_CALLING { // Put DV and extra gvCFs together -> send to glnexus BCFTOOLS_SORT_DV.out.vcf - .concat(BCFTOOLS_VIEW_REGIONS.out.vcf) .map { meta, gvcf -> [ ['id':'multisample'], gvcf ]} .groupTuple() .set{ ch_glnexus_in } @@ -91,8 +75,6 @@ workflow SHORT_VARIANT_CALLING { // Get versions ch_versions = ch_versions.mix(DEEPVARIANT.out.versions) ch_versions = ch_versions.mix(GLNEXUS.out.versions) - ch_versions = ch_versions.mix(BCFTOOLS_VIEW_REGIONS.out.versions) - ch_versions = ch_versions.mix(TABIX_EXTRA_GVCFS.out.versions) ch_versions = ch_versions.mix(TABIX_DV.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_CONCAT_DV.out.versions) ch_versions = ch_versions.mix(BCFTOOLS_SORT_DV.out.versions) diff --git a/subworkflows/local/short_variant_calling/tests/main.nf.test b/subworkflows/local/short_variant_calling/tests/main.nf.test index 771db68b..497bb74c 100644 --- a/subworkflows/local/short_variant_calling/tests/main.nf.test +++ b/subworkflows/local/short_variant_calling/tests/main.nf.test @@ -5,7 +5,7 @@ nextflow_workflow { config "./nextflow.config" workflow "SHORT_VARIANT_CALLING" - test("1 sample - no bed, [], fasta, fai, []") { + test("1 sample - no bed, fasta, fai, []") { when { workflow { @@ -16,16 +16,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true), [] ] - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [[],[]] + input[3] = [[],[]] """ } } @@ -39,7 +38,7 @@ nextflow_workflow { } - test("1 sample - 1 bed, [], fasta, fai, []") { + test("1 sample - 1 bed, fasta, fai, []") { when { workflow { @@ -50,16 +49,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true), file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [[],[]] + input[3] = [[],[]] """ } } @@ -74,7 +72,7 @@ nextflow_workflow { } - test("1 sample - 1 bed, [], fasta, fai, bed") { + test("1 sample - 1 bed, fasta, fai, bed") { when { workflow { @@ -85,16 +83,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true), file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [ + input[3] = [ [ id:'bed'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] @@ -112,7 +109,7 @@ nextflow_workflow { } - test("1 sample - 2 bed, [], fasta, fai, bed") { + test("1 sample - 2 bed, fasta, fai, bed") { when { workflow { @@ -131,16 +128,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed', checkIfExists: true) ] ) - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [ + input[3] = [ [ id:'bed'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] @@ -158,7 +154,7 @@ nextflow_workflow { } - test("2 samples - 2 bed, [], fasta, fai, bed") { + test("2 samples - 2 bed, fasta, fai, bed") { when { workflow { @@ -189,16 +185,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed', checkIfExists: true) ] ) - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [ + input[3] = [ [ id:'bed'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] @@ -216,7 +211,7 @@ nextflow_workflow { } - test("1 sample - no bed, [], fasta, fai, [] - stub") { + test("1 sample - no bed, fasta, fai, [] - stub") { options "-stub" @@ -229,16 +224,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true), [] ] - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [[],[]] + input[3] = [[],[]] """ } } @@ -253,7 +247,7 @@ nextflow_workflow { } - test("1 sample - 1 bed, [], fasta, fai, [] - stub") { + test("1 sample - 1 bed, fasta, fai, [] - stub") { options "-stub" @@ -266,16 +260,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true), file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [[],[]] + input[3] = [[],[]] """ } } @@ -290,7 +283,7 @@ nextflow_workflow { } - test("1 sample - 1 bed, [], fasta, fai, bed - stub") { + test("1 sample - 1 bed, fasta, fai, bed - stub") { options "-stub" @@ -303,16 +296,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true), file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [ + input[3] = [ [ id:'bed'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] @@ -330,7 +322,7 @@ nextflow_workflow { } - test("1 sample - 2 bed, [], fasta, fai, bed - stub") { + test("1 sample - 2 bed, fasta, fai, bed - stub") { options "-stub" @@ -351,16 +343,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed', checkIfExists: true) ] ) - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [ + input[3] = [ [ id:'bed'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] @@ -378,7 +369,7 @@ nextflow_workflow { } - test("2 samples - 2 bed, [], fasta, fai, bed - stub") { + test("2 samples - 2 bed, fasta, fai, bed - stub") { options "-stub" @@ -411,16 +402,15 @@ nextflow_workflow { file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed', checkIfExists: true) ] ) - input[1] = Channel.empty() - input[2] = [ + input[1] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'genome'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) ] - input[4] = [ + input[3] = [ [ id:'bed'], file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true) ] diff --git a/subworkflows/local/short_variant_calling/tests/main.nf.test.snap b/subworkflows/local/short_variant_calling/tests/main.nf.test.snap index 65c71b94..d4f2085b 100644 --- a/subworkflows/local/short_variant_calling/tests/main.nf.test.snap +++ b/subworkflows/local/short_variant_calling/tests/main.nf.test.snap @@ -1,5 +1,5 @@ { - "1 sample - 1 bed, [], fasta, fai, bed - stub": { + "1 sample - 1 bed, fasta, fai, bed": { "content": [ { "0": [ @@ -8,7 +8,7 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" ] ], "1": [ @@ -16,18 +16,18 @@ { "id": "multisample" }, - "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" + "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" ] ], "2": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -37,7 +37,7 @@ { "id": "multisample" }, - "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" + "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" ] ], "snp_calls_vcf": [ @@ -46,18 +46,18 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" ] ], "versions": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -68,9 +68,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-28T09:08:56.289754473" + "timestamp": "2024-06-28T17:46:21.204025161" }, - "1 sample - 1 bed, [], fasta, fai, [] - stub": { + "2 samples - 2 bed, fasta, fai, bed": { "content": [ { "0": [ @@ -79,7 +79,14 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + ], + [ + { + "id": "test2", + "single_end": false + }, + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,0ce965213b3c4fa10f5e855e0254cc0e" ] ], "1": [ @@ -87,18 +94,33 @@ { "id": "multisample" }, - "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" + "multisample.bcf:md5,b64046133e7bb7c6c9bc5bacfc4592b3" ] ], "2": [ + "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", + "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,da8068d97833cacf511ffe498131adaa", + "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -108,7 +130,7 @@ { "id": "multisample" }, - "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" + "multisample.bcf:md5,b64046133e7bb7c6c9bc5bacfc4592b3" ] ], "snp_calls_vcf": [ @@ -117,18 +139,40 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + ], + [ + { + "id": "test2", + "single_end": false + }, + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,0ce965213b3c4fa10f5e855e0254cc0e" ] ], "versions": [ + "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", + "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,da8068d97833cacf511ffe498131adaa", + "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -139,9 +183,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-28T09:08:43.398200049" + "timestamp": "2024-06-28T17:47:23.709677761" }, - "1 sample - 1 bed, [], fasta, fai, bed": { + "1 sample - 1 bed, fasta, fai, []": { "content": [ { "0": [ @@ -210,9 +254,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-26T21:19:29.875734506" + "timestamp": "2024-06-28T17:45:52.755681185" }, - "1 sample - no bed, [], fasta, fai, []": { + "1 sample - 2 bed, fasta, fai, bed - stub": { "content": [ { "0": [ @@ -221,7 +265,7 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "1": [ @@ -229,18 +273,21 @@ { "id": "multisample" }, - "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" + "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "2": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -250,7 +297,7 @@ { "id": "multisample" }, - "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" + "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "snp_calls_vcf": [ @@ -259,18 +306,21 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "versions": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -281,9 +331,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-28T10:26:42.351783606" + "timestamp": "2024-06-28T17:48:17.863172363" }, - "2 samples - 2 bed, [], fasta, fai, bed": { + "2 samples - 2 bed, fasta, fai, bed - stub": { "content": [ { "0": [ @@ -292,14 +342,14 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ], [ { "id": "test2", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,0ce965213b3c4fa10f5e855e0254cc0e" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "1": [ @@ -307,7 +357,7 @@ { "id": "multisample" }, - "multisample.bcf:md5,b64046133e7bb7c6c9bc5bacfc4592b3" + "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "2": [ @@ -317,21 +367,21 @@ "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", @@ -343,7 +393,7 @@ { "id": "multisample" }, - "multisample.bcf:md5,b64046133e7bb7c6c9bc5bacfc4592b3" + "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "snp_calls_vcf": [ @@ -352,14 +402,14 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ], [ { "id": "test2", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,0ce965213b3c4fa10f5e855e0254cc0e" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "versions": [ @@ -369,21 +419,21 @@ "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", @@ -396,9 +446,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-26T21:21:54.809167844" + "timestamp": "2024-06-28T17:48:33.855199338" }, - "1 sample - 1 bed, [], fasta, fai, []": { + "1 sample - no bed, fasta, fai, []": { "content": [ { "0": [ @@ -467,9 +517,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-26T21:18:55.954908032" + "timestamp": "2024-06-28T17:45:24.32759434" }, - "1 sample - no bed, [], fasta, fai, [] - stub": { + "1 sample - 1 bed, fasta, fai, [] - stub": { "content": [ { "0": [ @@ -538,9 +588,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-28T10:28:58.425875863" + "timestamp": "2024-06-28T17:47:49.907226308" }, - "1 sample - 2 bed, [], fasta, fai, bed": { + "1 sample - 1 bed, fasta, fai, bed - stub": { "content": [ { "0": [ @@ -549,7 +599,7 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "1": [ @@ -557,21 +607,18 @@ { "id": "multisample" }, - "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" + "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "2": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -581,7 +628,7 @@ { "id": "multisample" }, - "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" + "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "snp_calls_vcf": [ @@ -590,21 +637,18 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "versions": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", + "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", + "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", - "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", - "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -615,9 +659,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-26T21:20:20.752118695" + "timestamp": "2024-06-28T17:48:03.133920517" }, - "2 samples - 2 bed, [], fasta, fai, bed - stub": { + "1 sample - no bed, fasta, fai, [] - stub": { "content": [ { "0": [ @@ -627,13 +671,6 @@ "single_end": false }, "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" - ], - [ - { - "id": "test2", - "single_end": false - }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "1": [ @@ -645,29 +682,14 @@ ] ], "2": [ - "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,da8068d97833cacf511ffe498131adaa", - "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -687,39 +709,17 @@ "single_end": false }, "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" - ], - [ - { - "id": "test2", - "single_end": false - }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "versions": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", - "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", - "versions.yml:md5,da8068d97833cacf511ffe498131adaa", - "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -730,9 +730,9 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-28T09:09:26.366065596" + "timestamp": "2024-06-28T17:47:36.537498338" }, - "1 sample - 2 bed, [], fasta, fai, bed - stub": { + "1 sample - 2 bed, fasta, fai, bed": { "content": [ { "0": [ @@ -741,7 +741,7 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" ] ], "1": [ @@ -749,21 +749,21 @@ { "id": "multisample" }, - "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" + "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" ] ], "2": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -773,7 +773,7 @@ { "id": "multisample" }, - "multisample.bcf:md5,d41d8cd98f00b204e9800998ecf8427e" + "multisample.bcf:md5,51e2a9de1a43bfb041040ad04c61b4db" ] ], "snp_calls_vcf": [ @@ -782,21 +782,21 @@ "id": "test", "single_end": false }, - "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + "concat_dv_vcf.vcf.gz_sorted.vcf.gz:md5,d8a35574801cfec345629770bfae2de7" ] ], "versions": [ "versions.yml:md5,38a1582fc609ee8377f3886a5ebca2d5", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", "versions.yml:md5,38fcec4389d89d9ebbdaa63f369600f6", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,7cb036286d153d15eea690e9a10a5ce4", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", - "versions.yml:md5,9745bff5dc75d55770369e32bc8f3ceb", "versions.yml:md5,9fc1f49f5f53c7632a62df4cc241fe87", - "versions.yml:md5,a892c0e39dcdd490a66509569ce4137c", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", + "versions.yml:md5,bde7cbe90248255755f0b32582174a9e", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", "versions.yml:md5,bf43ab2e9ad507743150a308745a1eff", + "versions.yml:md5,c21eaade044c570ce3384fb22ba4c5fb", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", + "versions.yml:md5,c69d0d4be6b316a859ac77cf0f38e0b0", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,da8068d97833cacf511ffe498131adaa", "versions.yml:md5,dce103ef6b2f37c6844db8191418b9e6" @@ -807,6 +807,6 @@ "nf-test": "0.8.4", "nextflow": "24.04.2" }, - "timestamp": "2024-06-28T09:09:10.021442882" + "timestamp": "2024-06-28T17:46:50.879572276" } } \ No newline at end of file diff --git a/workflows/nallo.nf b/workflows/nallo.nf index 914ed0e7..f572e3c1 100644 --- a/workflows/nallo.nf +++ b/workflows/nallo.nf @@ -65,8 +65,6 @@ workflow NALLO { : '' ch_extra_snfs = params.extra_snfs ? Channel.fromSamplesheet('extra_snfs') : Channel.empty() - ch_extra_gvcfs = params.extra_gvcfs ? Channel.fromSamplesheet('extra_gvcfs') - : Channel.empty() ch_tandem_repeats = params.tandem_repeats ? Channel.fromPath(params.tandem_repeats).map{ [ it.getSimpleName(), it]}.collect() : Channel.value([[],[]]) ch_bed = params.bed ? Channel.fromPath(params.bed).map{ [ it.getSimpleName(), it]}.collect() @@ -268,7 +266,7 @@ workflow NALLO { if(!params.skip_short_variant_calling) { // Call SNVs with DeepVariant/DeepTrio - SHORT_VARIANT_CALLING( ch_snv_calling_in , ch_extra_gvcfs, fasta, fai, ch_bed ) + SHORT_VARIANT_CALLING( ch_snv_calling_in, fasta, fai, ch_bed ) ch_versions = ch_versions.mix(SHORT_VARIANT_CALLING.out.versions) if(!params.skip_snv_annotation) {