buffer_creation_hilic.py

# Creates the equilibration, binding, and wash buffer buffer for the HILIC protocol
from opentrons import protocol_api
import math

metadata = {
    "protocolName": "Buffer Creation in the HILIC protocol",
    "author": "Nico To",
    "description": '''
    Creates the equilibration, binding, and wash buffer for the HILIC protocol
    Equilibration Buffer: 100mM ammonium acetate pH 4.5, 15%% acetonitrile
    Binding 'Buffer: 200 mM ammonium acetate pH 4.5, 30%% acetonitrile
    Wash Buffer: 95%% acetonitrile (5%% water)
    ''',
}

requirements = {"robotType": "Flex", "apiLevel": "2.19"}

def add_parameters(parameters: protocol_api.Parameters):
    parameters.add_int(
        variable_name="ammoniumAcetate_conc",
        display_name="Concentration of Ammonium Acetate Stock",
        description="_______ mM Ammonium Acetate (4.5pH)",
        default=300,
        minimum=100,
        maximum=1000,
        unit="mM"
    )
    
    parameters.add_float(
        variable_name="ammoniumAcetate_amt",
        display_name="Amount of Ammonium Acetate Stock",
        description="_______ ml Ammonium Acetate (4.5pH) in 50ml falcon",
        default=50,
        minimum=5,
        maximum=50,
        unit="ml"
    )

    parameters.add_float(
        variable_name="acn_amt",
        display_name="Amount of 100% Acetonitrile Stock",
        description="_______ ml 100% Acetonitrile in 50ml falcon",
        default=50,
        minimum=5,
        maximum=50,
        unit="ml"
    )
    
    parameters.add_float(
        variable_name="water_amt",
        display_name="Amount of H2O Stock",
        description="_______ ml water in 50ml falcon",
        default=50,
        minimum=5,
        maximum=50,
        unit="ml"
    )

    parameters.add_float(
        variable_name="eq_buff_amt",
        display_name="Equilibration Buffer Amount",
        description="Amount of equilibration buffer to create",
        default=5,
        minimum=1,
        maximum=15,
        unit="ml"
    )

    parameters.add_float(
        variable_name="binding_buff_amt",
        display_name="Binding Buffer Amount",
        description="Amount of binding buffer to create",
        default=5,
        minimum=1,
        maximum=15,
        unit="ml"
    )
    
    parameters.add_float(
        variable_name="wash_buff_amt",
        display_name="Wash Buffer Amount",
        description="Amount of wash buffer to create",
        default=5,
        minimum=1,
        maximum=15,
        unit="ml"
    )

def get_height_50ml_falcon(volume):
    '''
    Get's the height of the liquid in the tube
    Volume: volume of liquid in tube in µl
    Return: hieght from bottom of tube in millimeters
    '''
    #y=1.8x+12
    if volume >=5000 and volume <=50000:
        xxx
    if volume <5000:
        return 0