From c1f1fdc7cc11f85e669678522414749b5325fa8b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?P=C3=A1draic=20Corcoran?= Date: Wed, 17 Apr 2024 16:31:37 +0200 Subject: [PATCH] Revert "chore: dev to master (#113)" This reverts commit 60d9f7b57e5defe60c9f8b9b95d11e2556df01b4. --- .github/workflows/test-build-docs.yaml | 34 - .readthedocs.yaml | 20 - .tests/integration/config.yaml | 8 - .../1000g.phase3.10k.b38.exome.vcf.gz.dat.UD | 9892 ----------------- .../1000g.phase3.10k.b38.exome.vcf.gz.dat.V | 2504 ----- .../1000g.phase3.10k.b38.exome.vcf.gz.dat.bed | 9892 ----------------- .../1000g.phase3.10k.b38.exome.vcf.gz.dat.mu | 9892 ----------------- README.md | 4 - config/config.yaml | 7 - docs/extra.css | 50 - docs/images/generate_dag.txt | 5 - docs/images/qc.dot | 38 - docs/images/qc.png | Bin 74250 -> 0 bytes docs/includes/abbreviations.md | 25 - docs/index.md | 8 - docs/intro.md | 41 - docs/requirements.txt | 10 - docs/softwares.md | 325 - images/qc.svg | 212 +- mkdocs.yml | 34 - requirements.txt | 3 +- workflow/Snakefile | 1 - workflow/rules/common.smk | 11 +- workflow/rules/gatk.smk | 2 +- workflow/rules/peddy.smk | 10 +- workflow/rules/picard.smk | 10 +- workflow/rules/samtools.smk | 10 +- workflow/rules/verifybamid2.smk | 33 - workflow/schemas/config.schema.yaml | 30 +- workflow/schemas/resources.schema.yaml | 42 +- workflow/schemas/rules.schema.yaml | 479 - 31 files changed, 144 insertions(+), 33488 deletions(-) delete mode 100644 .github/workflows/test-build-docs.yaml delete mode 100644 .readthedocs.yaml delete mode 100644 .tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.UD delete mode 100644 .tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.V delete mode 100644 .tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.bed delete mode 100644 .tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.mu delete mode 100644 docs/extra.css delete mode 100644 docs/images/generate_dag.txt delete mode 100644 docs/images/qc.dot delete mode 100644 docs/images/qc.png delete mode 100644 docs/includes/abbreviations.md delete mode 100644 docs/index.md delete mode 100644 docs/intro.md delete mode 100644 docs/requirements.txt delete mode 100644 docs/softwares.md delete mode 100644 mkdocs.yml delete mode 100644 workflow/rules/verifybamid2.smk delete mode 100644 workflow/schemas/rules.schema.yaml diff --git a/.github/workflows/test-build-docs.yaml b/.github/workflows/test-build-docs.yaml deleted file mode 100644 index 6eab04f..0000000 --- a/.github/workflows/test-build-docs.yaml +++ /dev/null @@ -1,34 +0,0 @@ - -name: build mkdocs - -on: - push: - branches: - - develop - - master - pull_request: - branches: - - develop - - master - workflow_dispatch: - -jobs: - build-mkdocs: - name: build mkdocs - runs-on: ubuntu-22.04 - steps: - - uses: actions/checkout@v3 - - name: Set up Python 3.8 - uses: actions/setup-python@v3 - with: - python-version: 3.8 - - name: Install requirements.txt - run: | - pip install -r requirements.txt - - name: Install requirements.test.txt - run: | - pip install -r requirements.test.txt - pip install -r docs/requirements.txt - - name: build mkdocs - run: | - mkdocs build \ No newline at end of file diff --git a/.readthedocs.yaml b/.readthedocs.yaml deleted file mode 100644 index 0688f13..0000000 --- a/.readthedocs.yaml +++ /dev/null @@ -1,20 +0,0 @@ -# .readthedocs.yaml -# Read the Docs configuration file -# See https://docs.readthedocs.io/en/stable/config-file/v2.html for details - -# Required -version: 2 - -# Set the version of Python and other tools you might need -build: - os: ubuntu-22.04 - tools: - python: "3.11" - -mkdocs: - configuration: mkdocs.yml - -# Optionally declare the Python requirements required to build your docs -python: - install: - - requirements: docs/requirements.txt \ No newline at end of file diff --git a/.tests/integration/config.yaml b/.tests/integration/config.yaml index 1b02918..6460101 100644 --- a/.tests/integration/config.yaml +++ b/.tests/integration/config.yaml @@ -36,8 +36,6 @@ multiqc: included_unit_types: ['N', 'T'] qc_files: - "qc/fastqc/{sample}_{type}_{flowcell}_{lane}_{barcode}_{read}_fastqc.zip" - - "qc/peddy/peddy.sex_check.csv" - - "qc/peddy/peddy.ped_check.csv" - "qc/picard_collect_alignment_summary_metrics/{sample}_{type}.alignment_summary_metrics.txt" - "qc/picard_collect_duplication_metrics/{sample}_{type}.duplication_metrics.txt" - "qc/picard_collect_gc_bias_metrics/{sample}_{type}.gc_bias.summary_metrics" @@ -91,9 +89,3 @@ rseqc_gene_body_coverage: rseqc_inner_distance: bed: "reference/gene_model.bed" container: "docker://hydragenetics/rseqc:4.0.0" - -verifybamid2: - container: "docker://hydragenetics/verifybamid2:2.0.1" - svd_mu: "verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.mu" - - diff --git a/.tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.UD b/.tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.UD deleted file mode 100644 index 000b927..0000000 --- a/.tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.UD +++ /dev/null @@ -1,9892 +0,0 @@ -12.9725 -3.63367 -2.84459 -0.29684 1.05918 0.47123 0.953943 -0.763596 -0.377155 -0.137909 -4.02375 -1.75411 -1.66905 -1.11804 0.0148311 -0.149117 0.254592 0.561472 0.276899 0.580447 --0.0103642 -0.0274306 0.01326 -0.018384 0.0134284 0.0276165 -0.0163701 0.00443246 -0.0327239 0.000859063 -2.27939 4.53707 -1.20388 1.05311 0.128261 -0.224966 0.0416705 -0.0713082 0.242734 -0.0673482 --5.67543 4.75144 -1.32992 0.712501 0.846635 -0.575778 1.79286 0.262567 0.141958 -1.5481 --2.70586 1.21734 1.40142 0.233118 -0.100503 -0.738681 -0.27311 0.434026 -0.477335 0.631455 -14.6285 10.0237 -3.92717 4.38528 -2.50393 2.09952 2.43915 0.273969 0.592771 -0.734365 -14.5058 10.1826 -3.91245 4.36365 -2.55051 2.10825 2.6444 0.292124 0.507263 -0.845044 -0.730002 1.0227 -0.259263 -0.246298 -0.154324 0.106423 0.368884 0.296687 -0.15508 -0.127252 --6.52929 -7.52717 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0.873733 --5.32824 -11.5833 10.8384 2.76203 -1.07283 0.786387 -1.86156 0.384853 0.129065 -0.349577 --17.3734 -13.3536 9.31698 4.5534 -0.460441 1.36531 -0.353411 0.557419 0.272328 0.309264 --7.51439 10.0771 -3.05762 -4.55409 -0.255087 -0.623467 -0.101859 -0.397499 -0.824659 -1.42689 -22.4738 1.88574 -5.17976 2.1044 -0.0859045 1.1126 0.0411997 0.334507 -0.740384 1.01538 --5.16631 4.05815 2.10671 -2.31614 1.72608 0.887548 -1.02147 -0.165296 0.276639 -0.464649 --6.02502 -2.70656 -9.90992 -0.484754 -1.69122 1.29168 0.00684411 -0.517676 -0.343203 -0.0436629 --14.9295 -1.75217 -0.782262 -0.0447701 -0.0452556 -0.250731 -0.340387 -0.19139 0.402019 1.07021 -8.83347 -5.14188 -8.16191 -1.12269 -1.52623 1.04122 1.497 1.71827 -1.46806 -2.20414 --1.04352 2.29268 -1.9303 0.105727 -1.07265 0.152487 0.304104 0.131368 -0.776013 -0.065234 --1.69452 0.435902 -3.6029 -2.01416 -2.24881 2.11055 0.480775 -0.591031 -0.12392 -0.732111 --3.44159 2.26782 -1.53162 -1.21863 -1.13524 -0.984466 0.278216 -0.118809 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0.0334149 -0.299293 -0.264284 0.189052 -0.252951 --6.64694 3.71659 4.29358 3.34832 0.825369 1.14845 -0.343285 -0.170142 -0.69732 0.984728 --6.67119 3.73854 4.32616 3.35357 0.83079 1.12319 -0.318747 -0.1561 -0.619634 0.98234 --8.89729 -5.48672 -2.89401 9.79415 -1.70758 -0.0928921 0.91342 -0.470714 -0.427172 0.0215349 --19.872 6.20285 0.633298 -6.83471 1.94615 0.370775 -0.501915 0.46063 -0.1159 -0.569862 -7.34007 0.796323 0.403194 0.032029 -0.0622165 0.0546306 0.0685421 -0.150068 0.489604 0.695231 --7.90632 -4.96793 2.40179 -4.43441 1.5883 0.17851 0.239038 -0.228906 0.292366 -0.330096 --1.70413 -8.404 1.90854 0.631616 0.522818 0.995867 1.23656 0.561876 -1.86066 -3.25847 --6.9262 8.83273 -4.81062 -0.613378 -1.06047 0.394228 -0.250106 0.368212 1.17415 1.01875 --6.1736 -0.207766 3.38989 6.46914 -1.18159 -0.390887 0.114548 0.0558805 0.415011 -0.171377 --4.82468 -1.39683 -3.98587 0.648706 -0.485565 -0.434409 -0.101363 0.594992 -0.284794 -0.103031 -2.52248 0.284688 0.134824 -0.0432027 0.166809 0.00552287 -0.613069 -0.962287 0.245141 0.523936 --4.62909 -0.632355 1.57786 -1.33804 1.64397 0.0883587 -1.95777 0.182268 -0.107971 -0.00886338 -1.8594 -1.05192 0.573515 -1.57066 -0.228425 0.791316 0.683979 0.375523 -0.152273 0.377299 -2.31191 0.268495 0.130168 0.0705807 -0.0206935 0.0382067 -0.175513 -0.12418 -0.00619422 -0.67946 --4.12647 4.37766 -1.87504 -2.23911 1.12171 -1.4804 -0.609976 -0.0442533 0.259538 0.113121 -0.278906 -2.30124 0.410226 2.64487 -0.282869 -0.920749 0.637151 0.120061 -0.282935 1.38207 -2.9715 -7.10627 -1.27614 3.1872 -0.682945 0.600923 -0.75597 0.272844 1.1208 -1.45633 -7.29266 0.843578 0.282959 -0.173445 0.335494 0.149871 -1.3026 -1.0924 -0.331163 -0.317304 --11.6301 -1.34252 -2.24191 2.73076 -0.54142 -2.91152 0.0919558 -0.977996 -0.568258 -2.02895 --0.287901 0.510478 0.182474 -0.14897 0.19619 -0.0802799 0.0617756 0.0353652 0.0282038 0.0628078 -2.3787 0.312527 0.0872964 -0.00375933 -0.0463265 0.0799529 0.27766 0.281946 -0.11643 -0.349687 --2.35168 10.5476 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2.411 1.648 1.6635 -0.623884 -2.12354 -0.753042 -0.435603 0.700853 --2.34876 -0.277081 -0.190152 -0.0974768 0.023081 0.0519943 -0.188013 -0.00381462 0.0824854 0.372291 -4.76258 0.524024 0.297008 0.0466402 0.125952 0.048068 0.748753 0.0465423 -0.149909 -0.381058 --6.7553 1.54852 1.77285 2.5075 -3.20115 1.19724 -2.26741 -0.463277 -0.0948674 1.9022 --1.53848 -3.36479 0.599629 -0.0610719 -0.219631 -1.90693 0.331983 -0.180839 -0.410287 0.300989 -4.77785 5.90312 -1.46744 5.14751 1.11771 0.626649 -1.57895 -0.914199 -1.07173 -1.97815 -11.0467 6.67667 -1.05951 5.21557 1.15237 0.582706 -1.47635 -0.232645 -0.860085 -0.872008 -6.01937 0.563747 0.331599 -0.121227 0.104778 0.0372963 -1.00534 -1.05092 -0.229538 -1.44446 -7.65782 1.21378 3.72785 3.8677 0.782194 0.589823 -1.42846 -0.828189 0.152562 0.541698 --9.87507 4.00733 3.83843 3.87417 1.66368 -1.35617 0.000245833 -1.1417 1.76921 -0.395278 -3.95258 2.97011 -1.09236 -0.997915 -2.76358 0.425282 0.449658 -0.638931 0.631533 -0.838002 --11.272 2.69028 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0.559862 1.52332 --15.562 5.97749 5.39853 -1.81548 -0.81229 0.988931 2.21801 -0.299746 0.408141 0.799053 -12.4667 2.20538 -5.48394 3.00832 -1.8503 -1.62504 0.359995 0.588937 -1.58817 -1.50589 --3.96821 10.1623 -0.751228 0.649737 1.49414 0.483886 -1.04615 2.13755 0.241092 -1.18514 -11.9356 -0.852575 2.48368 2.15982 -2.27444 -0.570004 0.539704 -0.32265 0.975512 -2.69841 -4.92333 0.554058 0.17044 0.000465198 0.0997886 -0.0733904 -0.884457 -0.272005 0.151483 1.53808 -18.5011 -4.28462 4.70157 0.565008 -2.42786 -1.75024 -0.343224 -1.01108 0.904771 -1.23015 --9.7567 2.59097 2.9343 -0.683212 -2.58414 -3.255 0.378312 0.313093 -0.0455352 -0.836338 --2.13599 -0.752333 -2.2454 0.209265 0.0112654 0.139437 -0.36295 0.105891 -0.432471 0.0575368 -0.242709 0.611056 -0.184156 0.376408 -0.0684575 -0.6253 -0.117813 -0.033828 0.159788 0.00623097 --2.06796 -1.73395 2.10788 1.69925 0.182123 0.478429 -0.157683 0.0280122 -0.00316251 -0.226006 -5.43871 1.61106 -0.411511 -0.612157 -0.0722158 0.607457 -0.277867 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0.363323 --0.204917 16.7196 -1.06007 1.96329 -0.369455 -1.4175 -0.350951 0.712526 0.345788 -0.880343 -0.814994 3.54709 -5.51454 2.50356 1.48336 -3.19652 0.545843 2.02989 0.960969 2.17766 -1.71711 0.107488 0.128005 -0.0672814 -0.0439902 -0.077333 0.218158 -0.0396949 0.155754 0.680153 -0.292375 -12.0978 -3.75235 0.901316 0.479268 1.20721 0.11831 -1.62017 1.04648 0.629771 -3.01947 -15.3346 -1.49089 -0.343798 0.567772 1.35186 0.354881 -1.6141 0.72383 -0.827792 --4.60243 -2.70394 -2.71852 -3.21104 -0.135003 2.09655 0.283055 0.730595 -0.867578 -1.64424 --3.72711 -2.66633 -0.456016 -0.274883 -0.882111 2.59053 -1.51533 -1.0595 0.0567067 -1.08098 --5.75078 4.4214 -1.08964 -1.11753 -0.717574 -0.758288 0.133302 0.535047 -0.0429239 -0.155164 --2.86811 -4.3502 -0.0708303 -1.88858 2.34316 -1.25071 0.132552 0.224018 0.107163 0.305383 -2.28235 0.226922 0.146549 0.0200223 0.0042505 -0.0528789 0.328478 -0.0581428 -0.195908 -0.358374 --22.8426 -0.925619 -1.35385 -0.134013 0.491534 0.319314 0.225101 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0.62423 -0.112107 -0.492353 -0.789334 0.994844 --0.590323 5.98918 -0.0185635 -2.19904 -0.0629588 -2.16753 -0.646327 0.0105398 0.525216 0.835413 -1.33001 0.159483 0.0848476 0.00807898 -0.0387321 0.101313 0.114072 -0.0376813 -0.0372915 -0.195192 -5.05977 0.107683 -1.23601 0.673363 -0.199056 -0.0644626 0.0595297 0.435399 0.182265 0.0605161 --10.6592 0.949391 1.58634 -2.60297 0.887661 -0.899868 -0.562215 0.461715 -0.227149 1.20932 -8.3938 2.56478 0.860637 -0.831739 0.692024 -1.21539 -2.72102 -0.436073 0.330618 3.32294 --12.9659 -0.815778 -5.51098 -6.37297 0.988275 -0.558712 0.403201 0.952694 -0.146245 0.0833851 -2.72172 0.321808 0.0859006 -0.0651599 -0.00418421 0.124618 0.181708 0.101635 -0.023889 -0.572981 -12.3228 9.22576 -1.03106 1.2126 1.2861 -0.631004 -0.246737 1.80532 -2.5687 -0.276825 --11.1474 -0.118186 0.17951 -0.0844417 0.146332 0.29427 0.951532 1.37101 0.0443664 0.194321 --6.37282 -0.652864 -0.35843 -0.000244587 0.150567 0.13859 0.670227 0.543449 -0.504619 -0.135728 --10.2197 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0.0253545 -0.186676 -0.108878 --1.41944 -1.11949 -7.92044 1.0877 1.12425 0.803778 0.14191 2.24662 -0.504616 0.403846 --5.36703 -1.6661 0.268451 1.87129 -1.04148 0.848076 -0.377194 -0.514388 -0.855941 -1.32746 --21.3257 8.41387 3.05892 -3.29419 -2.31067 0.198686 -1.33311 0.158184 -1.14964 -0.13705 -2.00121 -0.29268 -0.476396 0.191738 -0.0928544 0.23707 -0.0354008 -0.28348 0.170021 -0.721845 -1.44421 0.153511 0.0243752 0.00431581 0.0245401 0.0519117 0.277186 -0.00732189 -0.0888744 -0.0895519 --9.8332 -1.10325 -0.545771 0.0312417 -0.0747415 -0.00790575 -0.124372 -0.258458 -0.0420044 0.670551 --21.3048 -4.19388 -2.41414 5.6958 -0.424333 -2.16502 1.09417 -0.953272 0.417597 -0.613493 --21.9867 -2.89896 -3.48928 6.25595 -0.0500359 -1.40678 1.16885 -0.640241 0.46331 -0.280454 --1.12362 -1.68072 -0.172184 -0.739276 0.0139645 0.460973 0.14878 0.0451308 0.277477 0.201754 --4.79268 -2.53768 1.66097 3.41494 -0.134661 -0.0861877 -1.17054 -0.938227 1.4329 2.32554 --3.08107 1.67885 -0.689539 -0.111958 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0.041468 -0.725389 --19.7361 -7.32243 1.16338 1.87172 -0.279348 -1.0639 -0.406152 0.667261 0.797083 -1.03463 --0.192754 -1.12827 0.0408041 -0.536846 -1.06611 -0.192928 0.728964 0.451199 -0.504153 -0.0640266 --3.35205 -0.348869 -0.202331 0.00580347 0.0928345 -0.00595067 -0.457558 -0.672225 0.114264 0.157663 -1.82877 0.223364 0.0774424 0.00943439 0.0032232 0.0647001 -0.436185 -0.627776 -0.103703 -0.381371 -1.01324 0.123845 0.0655392 -0.0274274 -0.0505005 -0.0153755 -0.451779 -0.204125 0.147238 0.174693 --14.3857 8.87279 -0.780756 -0.0467335 1.52251 0.014536 0.901338 -1.40136 1.00173 1.17867 --2.19616 -5.01755 2.39101 -0.993848 -1.36967 -2.08597 -1.4997 0.139593 -0.255493 0.725072 --14.4664 7.78465 0.625687 -0.48066 0.86907 0.356906 1.20816 -0.803334 1.08758 1.62198 --1.33899 -6.62591 3.28511 1.69785 -0.179532 -0.113542 1.35993 0.772019 -0.438431 -0.262006 -2.22421 0.249478 0.0995728 -0.00833094 -0.0204839 0.0412198 0.124315 0.0493106 0.00925251 -0.0936696 --0.160248 -0.438372 0.159978 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0.191904 -0.257197 0.0545065 0.43088 -0.192567 1.1057 --5.87558 4.58222 6.87103 -0.196727 -0.2494 1.32559 1.40516 0.740305 0.723846 1.14123 --7.08011 16.3701 -3.15267 0.346416 0.348369 0.300652 -0.696038 -1.42213 -0.312353 0.675235 --2.16824 5.04361 4.57884 5.43281 0.874663 0.00858348 0.0994053 -0.177794 0.812363 -0.711337 -3.24482 -3.90997 0.278125 -1.47172 -2.01338 -4.55924 0.540027 -0.198429 0.112885 -0.246131 --19.4561 11.2046 -4.60716 2.84419 2.44965 1.88498 -0.421341 0.590914 -0.114496 1.06915 --4.10222 3.68663 -0.00297217 4.2213 0.647705 0.559666 0.314466 0.362537 0.105725 0.717128 --5.11402 3.37438 -1.79342 0.523204 -1.86111 1.35476 -0.813666 0.0879349 -0.257608 0.749175 --2.59444 4.15909 9.56929 -2.7128 -0.281465 0.485113 -0.369835 0.263649 0.77922 0.169836 --9.6667 7.50443 -3.84606 -1.25791 2.86538 0.56604 0.244337 0.265901 1.03159 -0.483883 -10.4157 -3.25831 -10.0238 -7.57588 -2.05081 1.44761 0.448214 -1.23306 -1.52816 -0.0766157 -17.208 1.50598 2.60874 2.98628 0.65098 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1.8177 0.704752 4.72181 -2.36812 -0.660166 -0.581644 -0.488894 -0.937572 0.00137655 --11.908 3.41674 7.8523 -3.14145 -1.46963 -0.429894 0.199614 -0.583777 -0.344724 0.431961 -3.47807 0.422875 0.149513 -0.11043 0.12713 0.234215 0.299897 0.330422 -0.151794 -0.564549 --1.18019 -0.840762 0.52468 1.60495 0.038273 -0.287551 -0.127186 -0.119529 -0.187312 -0.0202648 -3.45871 0.382918 0.104242 -0.0827669 0.0518325 0.252296 0.278778 0.329977 -0.234265 -0.533822 -0.387044 -0.68187 -0.104354 -1.08024 0.0881793 -0.0705627 -0.768557 -0.303975 0.0699784 -0.537681 -0.717691 -12.4861 -0.248421 4.76494 0.806454 -1.91217 2.07496 0.670505 -0.0610122 -1.63542 --3.32107 -7.22644 4.90152 4.89105 0.704507 -0.261486 1.85385 -0.214022 0.23748 -0.868827 --0.759592 1.52257 0.196246 -0.586684 0.86047 0.0277223 0.147595 -0.0415819 0.143982 -0.126888 -4.98846 0.476334 0.333235 0.0336942 0.0160641 -0.0857459 -0.47584 -0.775185 0.0992346 0.0184409 -3.84357 0.60788 0.251375 -0.0380608 -0.0305404 -0.160985 -0.634723 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0.160749 --1.84207 4.80835 -0.249618 1.06457 2.38336 3.4543 -1.45669 -1.67577 0.170293 -1.60939 -5.26259 -4.04795 1.14672 -1.48504 0.233116 0.74604 0.328156 1.03374 0.193466 0.31203 -4.18118 2.06379 -4.94507 -1.56044 -0.953236 -2.03298 1.96822 0.662485 0.438043 -0.269202 --8.07918 8.35495 3.47898 4.90274 -0.297666 0.333857 -1.26144 -0.430455 -0.0477431 1.21365 -8.44385 8.81299 2.28397 1.8556 -1.4548 0.277988 -0.656061 0.971302 -0.129653 0.27448 --4.67902 3.70786 -0.0256449 2.54675 -3.32679 0.722619 -0.835415 0.606882 -0.593315 -0.533321 -11.0576 1.13781 0.586856 0.0318707 0.102965 -0.0898484 -0.53846 -0.371886 -0.211842 1.2048 -6.71133 -8.09916 5.40631 1.66125 -2.50352 0.178433 0.129634 -0.0684448 0.0061608 0.00959867 -1.53597 0.250754 0.341717 -1.4318 -1.97919 -0.0253677 0.212155 1.37894 -0.819941 -1.17238 --2.60971 -4.63723 -3.17922 1.33514 -0.141469 1.00945 1.54023 1.89399 -0.92564 0.308548 -10.8493 1.18301 0.631245 0.16005 0.164042 0.384337 -0.880408 -0.513323 -0.0756443 -0.485443 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0.0211289 0.00514909 0.0236599 -0.00864073 --5.28652 6.79902 -4.82156 -1.24436 1.99185 -1.29886 0.236277 0.981152 -1.08469 0.490211 --5.8436 5.67239 -4.27603 -2.03712 1.90087 -0.379959 0.322981 0.844738 -1.15984 0.462808 -11.1551 1.26238 0.373232 0.0991032 -0.124993 -0.231325 1.61528 1.19234 0.304964 0.547794 --1.30877 -4.20683 1.85067 -1.8505 0.00137396 -0.00426931 0.250167 0.345856 0.482544 0.127533 --9.71815 0.370247 0.294019 8.61338 0.781609 -0.661211 -0.309643 -0.162005 1.15156 1.08938 --8.5466 3.27408 -0.192152 5.26506 -2.75053 -0.275454 0.798187 0.196973 -0.368953 -0.374498 --8.29436 -7.60578 -2.14908 4.94024 -3.44592 -2.69007 0.808354 1.13112 -0.935521 -0.631713 -12.6372 2.12992 0.934683 -0.497421 0.0459219 0.731369 -0.153927 0.117761 -0.917192 -1.65495 --2.47021 4.1729 0.306556 -5.11932 -1.27474 0.09371 -0.0537976 -0.784748 0.0173061 0.00062641 --0.669899 -1.79297 0.486265 -0.670288 0.0971859 0.292362 0.0631773 -0.235074 -0.118208 0.437566 -3.52897 0.364197 0.244791 0.0370269 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0.47379 0.226744 -0.277892 --11.6873 14.266 -8.36837 4.28999 0.122826 -0.344101 0.0282739 0.17505 0.391323 2.64214 --0.715378 -1.37715 -0.148951 -0.255087 -0.0616651 0.596415 -0.0734682 -0.0913803 0.0910867 0.11088 -5.73243 -16.2826 7.86432 -3.83915 -0.404992 0.367399 -0.426368 -0.391825 -0.0445254 -3.32567 -2.00014 -3.05575 -2.88788 0.454719 -0.71185 -0.234949 0.616011 0.495923 -0.223596 -0.312761 --7.25184 12.8986 3.94972 0.986734 -0.0702071 0.884956 -0.191853 0.708059 0.0837102 -1.01909 -10.5602 -2.05531 8.6735 -0.415699 0.200626 0.786511 -0.677808 0.713213 0.190013 -0.127459 -7.62928 -2.35987 8.98802 -0.304811 -0.0958628 1.22035 -1.39211 -0.140889 0.519389 -1.1568 -1.39454 3.2975 3.9428 -1.82838 -2.54619 -0.181621 0.430951 1.0847 1.14844 1.89206 --1.58517 1.17835 -2.25425 2.4235 1.56381 -0.0808743 -0.115483 0.850672 -0.0167385 -0.234728 -4.87389 0.35186 0.354619 -0.0420845 -0.0791472 -0.138356 0.359533 0.173146 0.00314587 -0.463116 --4.67604 2.60284 3.37896 -2.13517 -2.2779 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1.46407 -1.15121 0.0581313 0.267172 -0.0848626 -0.136169 0.0858206 0.183689 --19.8825 7.2163 1.46171 3.0697 -0.333095 1.1305 0.891141 0.626412 -1.65937 -0.15357 -0.148853 0.38392 -0.214399 0.152361 -0.0117639 -0.0165098 0.0088188 0.0354074 0.162642 -0.00680413 -7.42806 -2.68016 -4.99238 -9.31845 0.638164 -0.210914 -1.09866 0.576145 -0.689037 0.0926241 -4.75222 7.05181 -5.00653 6.30596 -2.54521 -0.239861 0.313066 -0.282687 0.107567 -0.135696 -15.4648 -9.85145 -3.30576 -1.16916 0.297861 -0.77907 0.596163 0.847334 1.98044 2.0185 --4.00787 -0.262551 -5.5152 -2.29458 2.35672 -0.224525 -0.919954 -0.175424 -0.959448 -1.14757 -3.97493 -3.07481 -7.04308 -2.59807 2.4544 -0.761872 -0.433272 0.255627 0.404467 -0.399433 -7.58206 -2.71372 -0.895703 -0.381068 0.318987 -0.419573 0.486041 0.443098 1.4433 0.449633 -6.24313 -17.7909 -3.6322 -0.259179 -0.48334 0.00766609 0.37015 0.876592 -1.58662 0.973283 -17.7654 -11.7163 -3.9668 1.99415 -1.24399 1.12982 0.0415397 0.821251 -0.309835 1.53813 --3.5911 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0.189344 0.364225 --6.51854 3.52099 0.225082 0.0589286 -0.337904 -0.755823 0.77544 -0.967772 0.895417 -0.43069 --2.86879 4.39788 0.906687 -2.32325 0.172688 0.0165477 0.788241 -0.326191 0.662342 -0.372454 --9.69468 9.49393 -3.42688 -2.43071 -3.63012 0.708217 14.2871 -13.2515 6.36478 0.69024 --6.12355 10.0281 -3.2388 -2.43037 -3.84796 0.761602 15.5101 -13.8456 6.27608 0.597174 --6.17618 10.1561 -3.27107 -2.43741 -3.78404 0.793264 15.5452 -13.8858 6.30121 0.574608 --2.2923 1.69446 3.92971 4.88162 1.46795 -0.522863 -1.29964 1.47744 0.119052 -0.185196 --2.41415 -1.75809 -3.269 6.19472 -0.949411 0.440318 0.680904 -0.933571 -0.891253 0.0385176 --6.51321 -9.24288 -1.94174 -2.24256 0.953976 2.50148 -4.54221 5.04823 -1.89598 -0.281729 --2.10166 14.9538 -3.47287 -4.18937 -3.71828 -0.252461 17.3405 -16.0719 6.42793 1.18869 -2.07526 15.5852 -5.68642 2.23175 -4.39257 1.35954 17.3457 -17.7513 4.74651 0.716777 --9.96719 9.69355 -3.72377 -2.38782 -3.7784 0.668792 14.5393 -13.5285 6.62012 0.701617 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1.77439 -1.43507 -1.29798 --24.9131 -3.31442 -2.03096 -2.23361 -0.663358 0.457101 -0.107367 0.845316 0.214112 -0.357516 -2.72652 0.356947 0.0482717 -0.01113 0.0397026 0.139603 -0.117516 -0.0957371 0.0224523 0.246379 -3.74373 0.374922 0.221315 0.00373848 -0.0467871 0.0734035 0.46348 0.569025 -0.22807 -0.277486 -2.43516 0.244437 0.146732 0.00782875 -0.0252139 0.0578293 0.139841 0.260769 -0.0428342 -0.239997 -2.53508 0.25581 0.157684 -0.0133075 -0.0319607 0.0483455 0.149542 0.320908 -0.0651632 -0.299812 --2.2933 6.50142 2.54642 0.0110819 0.40054 2.24544 -0.966848 -1.67558 -0.336876 -2.04026 -6.18899 6.03795 -0.222675 -1.87283 -0.206176 -0.690875 0.650163 0.51665 0.00424744 1.71913 -5.07959 4.13271 -0.17772 0.172231 -0.110679 -0.646755 -0.00593039 0.855683 -1.095 -0.15296 --14.1601 5.69764 1.30458 0.216727 -0.954758 -0.173718 -0.151377 -1.70044 1.89395 -0.062745 --21.4386 3.09151 2.56024 -0.970129 0.0538051 0.0830631 0.329115 -0.590278 1.29166 0.400886 -15.7903 1.12991 2.11862 0.802157 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3.7642 1.22685 0.391244 0.472156 0.602135 -1.3831 1.22142 0.495351 --10.8342 3.98025 3.12529 1.14845 0.700259 0.608471 -0.297846 -2.05486 1.29563 1.27402 --0.13539 5.12615 3.77023 1.19764 0.49935 0.464508 0.617978 -1.34886 1.07571 0.431913 --0.804259 -0.316095 0.816102 0.545956 -0.467379 -0.450816 -0.461711 -0.0777195 0.016635 0.145453 --6.84972 7.27348 -0.304886 -0.618785 -0.127993 0.123757 0.355943 0.0278441 -0.298317 0.0326638 --14.7395 -9.25205 7.47454 0.235338 0.180183 -2.17478 -0.599279 -0.16763 0.188343 1.10801 -11.6148 1.38134 0.53422 0.00423327 -0.0270174 0.422298 -0.571943 -0.570757 0.311737 -0.810284 --2.89202 2.35475 1.48475 -0.174603 -1.61338 0.100183 -0.973305 -0.052397 -0.624542 0.529725 -0.610003 6.7369 -0.326585 -3.17376 -1.73648 0.403027 -1.00117 -1.48317 -0.747686 0.0301921 --7.41717 4.0706 -0.957028 3.12747 -0.483027 0.242354 -0.862417 0.134859 -0.244144 0.311918 --0.597859 3.2028 -9.99097 2.05899 -0.644014 -0.427832 0.63556 0.665712 -0.568868 -0.00794072 --12.2199 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0.207899 --14.481 -12.5842 8.00763 7.53559 0.752794 2.49158 0.00335689 0.480424 -0.256398 0.594925 --4.79274 9.36735 0.334387 3.99723 -1.29155 1.97677 -0.307255 -0.260621 -1.28573 -1.25857 -8.01388 0.333106 7.48462 -2.79392 -0.923151 -1.17715 1.03452 1.03804 0.738302 0.0439718 --1.66461 -3.71547 0.892097 -1.24789 -0.208836 -0.36611 0.117509 0.162138 -0.152118 -0.0336203 -2.48109 -3.28562 1.09745 -1.31176 -0.118568 -0.17672 -0.40315 -0.0359174 -0.1957 0.166572 --0.718694 -3.57306 1.00135 -1.25275 -0.15962 -0.325357 -0.101724 -0.122654 -0.0887876 -0.236395 -19.132 3.61077 5.55387 -3.76274 -2.05291 -0.786684 1.23287 1.11133 0.0354735 -1.17434 -4.90969 -5.35866 -1.65815 -0.436939 -0.302244 -0.722812 1.09928 1.55652 -0.254624 -1.17236 -1.09089 5.0801 4.94649 -2.99485 -0.981058 0.258287 0.807986 0.331995 -0.05057 -2.52435 --1.82653 -0.43405 6.10047 -1.78516 -1.5261 -2.9663 0.0341974 -1.45803 0.880415 -2.93843 -0.517921 1.11441 0.173141 0.390743 0.154058 0.182557 -0.0141799 0.182279 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0.147881 0.210269 0.180528 0.5719 diff --git a/.tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.V b/.tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.V deleted file mode 100644 index 29e1fe2..0000000 --- a/.tests/integration/verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.V +++ /dev/null @@ -1,2504 +0,0 @@ -HG00096 -0.00934532 -0.0285322 0.0139627 -0.0240514 0.00165181 0.00925792 -0.00974456 0.00349031 0.0223958 -0.0251693 -HG00097 -0.0105492 -0.0300035 0.00782959 -0.0162958 0.0147195 0.00199866 -0.0229665 -0.000287406 0.0157828 -0.00367114 -HG00099 -0.0106384 -0.0250886 0.00962413 -0.0165426 -0.000828718 0.0263838 -0.0206387 0.0163013 0.0194342 -0.027439 -HG00100 -0.0102672 -0.0283912 0.00987838 -0.0152448 -0.00289898 0.02364 -0.029971 0.0140522 0.0289266 -0.0222436 -HG00101 -0.0108366 -0.0271183 0.0109671 -0.0139828 0.00450677 0.0138331 0.0109932 -0.0121049 0.00360593 0.0165312 -HG00102 -0.0104739 -0.0252184 0.0132152 -0.0216763 0.0145939 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1.61981 -chr9:137112039 0.341853 -chr9:137156786 0.28155 -chr9:137215822 1.79832 -chr9:137356366 1.84145 -chr9:137395309 0.0171725 -chr9:137478113 0.0163738 -chr9:137540635 0.0555112 -chr9:137914847 0.0215655 -chr9:137986862 0.843051 -chr9:138017218 0.79353 -chr9:138043731 0.915335 -chr9:138058673 0.0650958 diff --git a/README.md b/README.md index 6882cdb..31ecfdd 100644 --- a/README.md +++ b/README.md @@ -1,7 +1,3 @@ -

-https://hydra-genetics-qc.readthedocs.io -

- # hydra-genetics/qc #### Snakemake module containing steps for quality control of sequencing data diff --git a/config/config.yaml b/config/config.yaml index a7838df..d2fa855 100644 --- a/config/config.yaml +++ b/config/config.yaml @@ -75,10 +75,3 @@ picard_collect_multiple_metrics: picard_collect_wgs_metrics: container: "docker://hydragenetics/picard:2.25.0" - -verifybamid2: - container: "docker://hydragenetics/verifybamid2:2.0.1" - svd_mu: "verifybamid2/1000g.phase3.10k.b38.exome.vcf.gz.dat.mu" - - - diff --git a/docs/extra.css b/docs/extra.css deleted file mode 100644 index cac7c9e..0000000 --- a/docs/extra.css +++ /dev/null @@ -1,50 +0,0 @@ -.wy-nav-content { - max-width: 1600px !important; -} -h1,h2 { - margin-top: 50px; - margin-bottom: 10px !important; -} -h3 { - font-size: 18px; - margin-top: 30px; - margin-bottom: 10px !important; -} -h4 { - font-size: 17px; - font-style: italic; - margin-top: 30px; - margin-bottom: 10px !important; - -} -h5 { - font-size: 16px; - font-style: italic; - margin-top: 30px; - margin-bottom: 10px !important; - -} -p { - margin-top: 5px !important; -} -* { - margin-bottom: 0px !important; -} - -table td { - min-width: 10px; - max-width: 600px; -} -table td:last-child { - width: 100%; -} - -hr { - margin-top: 50px; - border: 1px solid black; -} - -.twemoji { - width: 20px; - color: black; -} diff --git a/docs/images/generate_dag.txt b/docs/images/generate_dag.txt deleted file mode 100644 index 9cb5350..0000000 --- a/docs/images/generate_dag.txt +++ /dev/null @@ -1,5 +0,0 @@ -# Generate a dag graph and place it here -cd .tests/integration -snakemake --rulegraph -s ../../workflow/Snakefile --configfile config.yaml > qc.dot -# move and modify dot-file manually and then -dot -Tpng qc.dot > qc.png diff --git a/docs/images/qc.dot b/docs/images/qc.dot deleted file mode 100644 index 486f985..0000000 --- a/docs/images/qc.dot +++ /dev/null @@ -1,38 +0,0 @@ -digraph snakemake_dag { - rankdir="LR" - graph[bgcolor=white, margin=0]; - node[shape=box, style=rounded, fontname=sans, fontsize=10, penwidth=2]; - edge[penwidth=2, color=grey]; - 0[label = "html report", color = "0.0 0.0 0.0", style="dotted"]; - 1[label = "multiqc", color = "0.35 0.6 0.85", style="rounded"]; - 2[label = "peddy", color = "0.04 0.6 0.85", style="rounded"]; - 4[label = "fastqc", color = "0.63 0.6 0.85", style="rounded"]; - 5[label = "picard:\nalignment_summary_metrics\nduplication_metrics\ngc_bias_metrics\nhs_metrics\ninsert_size_metric1s\nmultiple_metrics\nwgs_metrics", color = "0.42 0.6 0.85", style="rounded"]; - 9[label = "samtools:\nstats\nidxstats", color = "0.11 0.6 0.85", style="rounded"]; - 10[label = "mosdepth", color = "0.21 0.6 0.85", style="rounded"]; - 14[label = "rseqc:\ngene_body_coverage\ninner_distance", color = "0.00 0.6 0.85", style="rounded"]; - 16[label = "gatk_calculate_contamination", color = "0.60 0.6 0.85", style="rounded"]; - 17[label = "gatk_get_pileup_summaries", color = "0.49 0.6 0.85", style="rounded"]; - 18[label = "bam", color = "0.0 0.0 0.0", style="dotted"]; - 19[label = "fastq", color = "0.0 0.0 0.0", style="dotted"]; - 20[label = "vcf", color = "0.0 0.0 0.0", style="dotted"]; - 21[label = "verifybamid2", color = "0.2 0.9 0.45", style="rounded"]; - 20 -> 2 - 19 -> 4 - 18 -> 5 - 18 -> 9 - 18 -> 10 - 18 -> 14 - 18 -> 17 - 18 -> 21 - 1 -> 0 - 16 -> 1 - 5 -> 1 - 14 -> 1 - 2 -> 1 - 10 -> 1 - 9 -> 1 - 4 -> 1 - 17 -> 16 - 21 -> 1 -} diff --git a/docs/images/qc.png b/docs/images/qc.png deleted file mode 100644 index ce0d7967ea6c4bec5cc99d9a9f48109b922d36ac..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 74250 zcmagG2{@Jg_y2n%LxmJkGLwYJkaGkPd1g`8mi`COGAiXe2zeW|-@jj@ECN3E3w;>_MmPtmcKHL+6I)NTMq`H)0PAyLpKS z=)FiJQSx7-h>_D@U8)^EfBK6hgB5QQNdkIahf;a`*UTR$Cnv+%9)ApY`}S=$J{cKV zSXdbHU1Eda2|Kx9c4?_2RdEpWji1Z*YL5lXI-Iqx9qFdM9y&{?;Pq_7JNC*&{ZJ

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-picard_collect_wgs_metrics + +picard_collect_gc_bias_metrics - + 3->1 - - + + 4 - -fastqc + +samtools_stats - + 4->1 - - + + 5 - -mosdepth_bed + +picard_collect_alignment_summary_metrics - + 5->1 - - + + 6 - -picard_collect_duplication_metrics + +mosdepth_bed - + 6->1 - - + + 7 - -picard_collect_gc_bias_metrics + +picard_collect_multiple_metrics - + 7->1 - - + + 8 - -mosdepth + +mosdepth - + 8->1 - - + + 9 - -picard_collect_hs_metrics + +fastqc - + 9->1 - - + + 10 - -samtools_stats + +picard_collect_insert_size_metrics - + 10->1 - - + + 11 - -picard_collect_alignment_summary_metrics + +picard_collect_hs_metrics - + 11->1 - - + + 12 - -picard_collect_multiple_metrics + +picard_collect_duplication_metrics - + 12->1 - - + + 13 - -picard_collect_insert_size_metrics + +rseqc_inner_distance - + 13->1 - - + + 14 - -rseqc_inner_distance + +rseqc_gene_body_coverage - + 14->1 - - + + 15 - -rseqc_gene_body_coverage + +gatk_calculate_contamination - - -15->1 - - + + +15->0 + + 16 - -gatk_calculate_contamination + +gatk_get_pileup_summaries - - -16->0 - - + + +16->15 + + 17 - -gatk_get_pileup_summaries - - - -17->16 - - - - - -18 - -verifybamid2 - - - -18->0 - - + +peddy + + + +17->0 + + diff --git a/mkdocs.yml b/mkdocs.yml deleted file mode 100644 index bb99edd..0000000 --- a/mkdocs.yml +++ /dev/null @@ -1,34 +0,0 @@ -site_name: qc -nav: - - Introduction: intro.md - - Softwares: softwares.md - -theme: readthedocs -extra_css: [extra.css] -markdown_extensions: - - abbr - - attr_list - - pymdownx.details - - pymdownx.snippets: - auto_append: - - includes/abbreviations.md - base_path: ["docs/", "workflow/rules/"] - url_download: "True" - - mdx_spanner - - admonition - - pymdownx.emoji: - emoji_index: !!python/name:materialx.emoji.twemoji - emoji_generator: !!python/name:materialx.emoji.to_svg - -plugins: - - yaml-schema: - yaml_files: - - file: "workflow/schemas/config.schema.yaml" - tag: "CONFIGSCHEMA" - - file: "workflow/schemas/resources.schema.yaml" - tag: "RESOURCESSCHEMA" - - snakemake-rule: - rule_folders: - - 'workflow/rules' - schemas: - - 'workflow/schemas/rules.schema.yaml' diff --git a/requirements.txt b/requirements.txt index 907c1eb..c618cf2 100644 --- a/requirements.txt +++ b/requirements.txt @@ -3,7 +3,6 @@ hydra-genetics==0.15.0 jinja2==3.0.1 networkx==2.5 pandas>=1.3.1 -pulp<2.8 singularity==3.0.0 -snakemake>=7.13.0,<8 +snakemake==7.8.0 tabulate<0.9.0 diff --git a/workflow/Snakefile b/workflow/Snakefile index 19379a3..217ca9c 100644 --- a/workflow/Snakefile +++ b/workflow/Snakefile @@ -13,7 +13,6 @@ include: "rules/peddy.smk" include: "rules/picard.smk" include: "rules/rseqc.smk" include: "rules/samtools.smk" -include: "rules/verifybamid2.smk" rule all: diff --git a/workflow/rules/common.smk b/workflow/rules/common.smk index 52f80fc..d4f511b 100644 --- a/workflow/rules/common.smk +++ b/workflow/rules/common.smk @@ -60,19 +60,10 @@ def compile_output_list(wildcards): for qc_type, value in config.get("multiqc", {}).get("reports", {}).items(): if not set(value.get("included_unit_types", [])).isdisjoint(types): output_files.append("qc/multiqc/multiqc_{}.html".format(qc_type)) - files = { - "qc/gatk_calculate_contamination": ["contamination.table"], - # "qc/verifybamid2": ["selfSM", "ancestry"], - } - # Since it is not possible to create integration test without a large dataset verifybamid2 will not be subjected to integration - # testing and we can not guarantee that it will work - output_files += [ - "%s/%s_%s.%s" % (prefix, sample, unit_type, suffix) - for prefix in files.keys() + "qc/gatk_calculate_contamination/%s_%s.contamination.table" % (sample, unit_type) for sample in get_samples(samples) for unit_type in get_unit_types(units, sample) - for suffix in files[prefix] if unit_type != "R" ] output_files += [ diff --git a/workflow/rules/gatk.smk b/workflow/rules/gatk.smk index a4d4f79..e574cc6 100644 --- a/workflow/rules/gatk.smk +++ b/workflow/rules/gatk.smk @@ -6,8 +6,8 @@ __license__ = "GPL-3" rule gatk_get_pileup_summaries: input: - bam="alignment/samtools_merge_bam/{sample}_{type}.bam", bai="alignment/samtools_merge_bam/{sample}_{type}.bam.bai", + bam="alignment/samtools_merge_bam/{sample}_{type}.bam", sites=config.get("gatk_get_pileup_summaries", {}).get("sites", ""), variants=config.get("gatk_get_pileup_summaries", {}).get("variants", ""), output: diff --git a/workflow/rules/peddy.smk b/workflow/rules/peddy.smk index b2ff6bf..26abd0d 100644 --- a/workflow/rules/peddy.smk +++ b/workflow/rules/peddy.smk @@ -7,19 +7,19 @@ __license__ = "GPL-3" rule peddy: input: vcf="qc/peddy/all.vcf.gz", - tbi="qc/peddy/all.vcf.gz.tbi", ped="qc/peddy/all.ped", + tbi="qc/peddy/all.vcf.gz.tbi", output: - het_check=temp("qc/peddy/peddy.het_check.csv"), - pca=temp("qc/peddy/peddy.background_pca.json"), ped=temp("qc/peddy/peddy.peddy.ped"), ped_check=temp("qc/peddy/peddy.ped_check.csv"), + sex_check=temp("qc/peddy/peddy.sex_check.csv"), + het_check=temp("qc/peddy/peddy.het_check.csv"), ped_html=temp("qc/peddy/peddy.html"), ped_vs_html=temp("qc/peddy/peddy.vs.html"), - sex_check=temp("qc/peddy/peddy.sex_check.csv"), + pca=temp("qc/peddy/peddy.background_pca.json"), params: - extra=config.get("peddy", {}).get("extra", ""), prefix=lambda wildcards, input: os.path.split(input.ped)[0], + extra=config.get("peddy", {}).get("extra", ""), log: "qc/peddy/peddy.output.log", benchmark: diff --git a/workflow/rules/picard.smk b/workflow/rules/picard.smk index faa7aac..e2ba384 100644 --- a/workflow/rules/picard.smk +++ b/workflow/rules/picard.smk @@ -7,7 +7,6 @@ __license__ = "GPL-3" rule picard_collect_alignment_summary_metrics: input: bam="alignment/samtools_merge_bam/{sample}_{type}.bam", - bai="alignment/samtools_merge_bam/{sample}_{type}.bam.bai", ref=config.get("reference", {}).get("fasta", ""), output: metrics=temp("qc/picard_collect_alignment_summary_metrics/{sample}_{type}.alignment_summary_metrics.txt"), @@ -42,7 +41,6 @@ rule picard_collect_alignment_summary_metrics: rule picard_collect_duplication_metrics: input: bam="alignment/samtools_merge_bam/{sample}_{type}.bam", - bai="alignment/samtools_merge_bam/{sample}_{type}.bam.bai", output: metrics=temp("qc/picard_collect_duplication_metrics/{sample}_{type}.duplication_metrics.txt"), log: @@ -75,7 +73,6 @@ rule picard_collect_duplication_metrics: rule picard_collect_gc_bias_metrics: input: bam="alignment/samtools_merge_bam/{sample}_{type}.bam", - bai="alignment/samtools_merge_bam/{sample}_{type}.bam.bai", ref=config.get("reference", {}).get("fasta", ""), output: chart=temp("qc/picard_collect_gc_bias_metrics/{sample}_{type}.gc_bias.pdf"), @@ -110,12 +107,11 @@ rule picard_collect_gc_bias_metrics: rule picard_collect_hs_metrics: input: bam="alignment/samtools_merge_bam/{sample}_{type}.bam", - bai="alignment/samtools_merge_bam/{sample}_{type}.bam.bai", bait_intervals=config.get("reference", {}).get("design_intervals", ""), reference=config.get("reference", {}).get("fasta", ""), target_intervals=config.get("reference", {}).get("design_intervals", ""), output: - metrics=temp("qc/picard_collect_hs_metrics/{sample}_{type}.HsMetrics.txt"), + temp("qc/picard_collect_hs_metrics/{sample}_{type}.HsMetrics.txt"), params: extra=config.get("picard_collect_hs_metrics", {}).get("extra", "COVERAGE_CAP=5000"), log: @@ -175,10 +171,9 @@ rule picard_collect_insert_size_metrics: rule picard_collect_multiple_metrics: input: bam="alignment/samtools_merge_bam/{sample}_{type}.bam", - bai="alignment/samtools_merge_bam/{sample}_{type}.bam.bai", ref=config.get("reference", {}).get("fasta", ""), output: - metrices=expand( + expand( "qc/picard_collect_multiple_metrics/{{sample}}_{{type}}.{ext}", ext=config.get("picard_collect_multiple_metrics", {}).get("output_ext", ""), ), @@ -211,7 +206,6 @@ rule picard_collect_multiple_metrics: rule picard_collect_wgs_metrics: input: bam="alignment/samtools_merge_bam/{sample}_{type}.bam", - bai="alignment/samtools_merge_bam/{sample}_{type}.bam.bai", ref=config.get("reference", {}).get("fasta", ""), interval=config.get("reference", {}).get("wgs_intervals", ""), output: diff --git a/workflow/rules/samtools.smk b/workflow/rules/samtools.smk index 999b732..b3d6266 100644 --- a/workflow/rules/samtools.smk +++ b/workflow/rules/samtools.smk @@ -8,9 +8,13 @@ rule samtools_stats: input: bam="alignment/samtools_merge_bam/{sample}_{type}.bam", output: - stats=temp("qc/samtools_stats/{sample}_{type}.samtools-stats.txt"), + temp("qc/samtools_stats/{sample}_{type}.samtools-stats.txt"), params: - extra=config.get("samtools_stats", {}).get("extra", ""), + extra="%s -t %s" + % ( + config.get("samtools_stats", {}).get("extra", ""), + config.get("reference", {}).get("design_bed", ""), + ), log: "qc/samtools_stats/{sample}_{type}.samtools-stats.txt.log", benchmark: @@ -30,7 +34,7 @@ rule samtools_stats: message: "{rule}: calculate qc using samtools for {input.bam}" wrapper: - "v3.0.0/bio/samtools/stats" + "0.79.0/bio/samtools/stats" rule samtools_idxstats: diff --git a/workflow/rules/verifybamid2.smk b/workflow/rules/verifybamid2.smk deleted file mode 100644 index b516dd8..0000000 --- a/workflow/rules/verifybamid2.smk +++ /dev/null @@ -1,33 +0,0 @@ -__author__ = "Padraic Corcoran and Magdalena" -__copyright__ = "Copyright 2023, Padraic Corcoran and Magdalena" -__email__ = "padraic.corcoran@scilifelab.uu.se" -__license__ = "GPL-3" - - -rule verifybamid2: - input: - bam="alignment/samtools_merge_bam/{sample}_{type}.bam", - ref=config.get("reference", {}).get("fasta", ""), - svd_mu=config.get("verifybamid2", {}).get("svd_mu", ""), - output: - selfsm=temp("qc/verifybamid2/{sample}_{type}.selfSM"), - ancestry=temp("qc/verifybamid2/{sample}_{type}.ancestry"), - params: - extra=config.get("verifybamid2", {}).get("extra", ""), - log: - "qc/verifybamid2/{sample}_{type}.selfsm.log", - benchmark: - repeat("qc/verifybamid2/{sample}_{type}.output.benchmark.tsv", config.get("verifybamid2", {}).get("benchmark_repeats", 1)) - threads: config.get("verifybamid2", {}).get("threads", config["default_resources"]["threads"]) - resources: - mem_mb=config.get("verifybamid2", {}).get("mem_mb", config["default_resources"]["mem_mb"]), - mem_per_cpu=config.get("verifybamid2", {}).get("mem_per_cpu", config["default_resources"]["mem_per_cpu"]), - partition=config.get("verifybamid2", {}).get("partition", config["default_resources"]["partition"]), - threads=config.get("verifybamid2", {}).get("threads", config["default_resources"]["threads"]), - time=config.get("verifybamid2", {}).get("time", config["default_resources"]["time"]), - container: - config.get("verifybamid2", {}).get("container", config["default_container"]) - message: - "{rule}: estimate contamination from {input.bam}" - wrapper: - "v2.6.0-35-g755343f/bio/verifybamid/verifybamid2" diff --git a/workflow/schemas/config.schema.yaml b/workflow/schemas/config.schema.yaml index 5458d5c..6f65e2d 100644 --- a/workflow/schemas/config.schema.yaml +++ b/workflow/schemas/config.schema.yaml @@ -43,6 +43,12 @@ properties: container: type: string description: name or path to docker/singularity container + outdir: + type: string + description: path to output directory + tmp: + type: string + description: path to temporary directory gatk_calculate_contamination: type: object @@ -144,8 +150,8 @@ properties: type: string required: - qc_files - required: - - reports + required: + - reports peddy: type: object @@ -212,9 +218,7 @@ properties: description: name or path to docker/singularity container extra: type: string - description: | - parameters that should be forwarded - NOTE: Increase COVERAGE_CAP=5000 if you have high coverage data or RNA data + description: parameters that should be forwarded picard_collect_insert_size_metrics: type: object @@ -277,22 +281,6 @@ properties: type: string description: parameters that should be forwarded - - - verifybamid2: - type: object - description: parameters for verifybamid2 - properties: - benchmark_repeats: - type: integer - description: set number of times benchmark should be repeated - container: - type: string - description: name or path to docker/singularity container - extra: - type: string - description: parameters that should be forwarded - required: - samples - units diff --git a/workflow/schemas/resources.schema.yaml b/workflow/schemas/resources.schema.yaml index 19d6e97..7007753 100644 --- a/workflow/schemas/resources.schema.yaml +++ b/workflow/schemas/resources.schema.yaml @@ -48,7 +48,27 @@ properties: type: string description: max execution time - gatk_calculate_contamination: + fastqc: + type: object + description: resource definitions for fastqc + properties: + mem_mb: + type: integer + description: max memory in MB to be available + mem_per_cpu: + type: integer + description: memory in MB used per cpu + partition: + type: string + description: partition to use on cluster + threads: + type: integer + description: number of threads to be available + time: + type: string + description: max execution time + + gatk_get_pileup_summaries: type: object description: resource definitions for gatk_calculate_contamination properties: @@ -328,25 +348,5 @@ properties: type: string description: max execution time - verifybamid2: - type: object - description: resource definitions for verifybamid2 - properties: - mem_mb: - type: integer - description: max memory in MB to be available - mem_per_cpu: - type: integer - description: memory in MB used per cpu - partition: - type: string - description: partition to use on cluster - threads: - type: integer - description: number of threads to be available - time: - type: string - description: max execution time - required: - default_resources diff --git a/workflow/schemas/rules.schema.yaml b/workflow/schemas/rules.schema.yaml deleted file mode 100644 index 212f9e9..0000000 --- a/workflow/schemas/rules.schema.yaml +++ /dev/null @@ -1,479 +0,0 @@ -$schema: "http://json-schema.org/draft-04/schema#" -description: snakemake rule input and output files description file -type: object -properties: - fastqc: - type: object - description: input and output parameters for fastqc - properties: - input: - type: object - description: list of inputs - properties: - fastq: - type: string - description: List of fastq files - output: - type: object - description: list of outputs - properties: - html: - type: string - description: Html report containing sequencing qc - zip: - type: string - description: Zipped qc data that can be used as input to MultiQC - - - gatk_calculate_contamination: - type: object - description: input and output parameters for gatk_calculate_contamination - properties: - input: - type: object - description: list of inputs - properties: - pileups_table: - type: string - description: Pileup generated by gatk_get_pileup_summaries - output: - type: object - description: list of outputs - properties: - contamination_table: - type: string - description: | - Measure of sample contamination - NOTE: needs modification to be used as input to MultiQC - - gatk_get_pileup_summaries: - type: object - description: input and output parameters for gatk_get_pileup_summaries - properties: - input: - type: object - description: list of inputsw - properties: - bam: - type: string - description: Aligned bam file - bai: - type: string - description: Bam index file - sites: - type: string - description: Vcf-file with common SNPs for example from GNOMAD - variants: - type: string - description: Vcf-file with common SNPs for example from GNOMAD - output: - type: object - description: list of outputs - properties: - pileups_table: - type: string - description: Pileup file with allele information for selected sites - - mosdepth: - type: object - description: input and output parameters for mosdepth - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Aligned bam file - bai: - type: string - description: Bam index file - output: - type: object - description: list of outputs - properties: - bed: - type: string - description: Bed file with coverage per region - csi: - type: string - description: Bed coverage index file - glob: - type: string - description: Global statistics used by MultiQC - region: - type: string - description: Statistics for the regions specified (--by parameter) used by MultiQC - summary: - type: string - description: Summary statistics - - - mosdepth_bed: - type: object - description: input and output parameters for mosdepth_bed - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Aligned bam file - bai: - type: string - description: Bam index file - bed: - type: string - description: Bed file regions used for per-base coverage - output: - type: object - description: list of outputs - properties: - bed: - type: string - description: Bed file with coverage per region - bed_csi: - type: string - description: Bed coverage index file - coverage: - type: string - description: Coverage per base in the regions - coverage_csi: - type: string - description: Coverage index file - glob: - type: string - description: Global statistics used by MultiQC - region: - type: string - description: Statistics for the regions specified (--by parameter) used by MultiQC - summary: - type: string - description: Summary statistics - - multiqc: - type: object - description: input and output parameters for multiqc - properties: - input: - type: object - description: list of inputs - properties: - files: - type: string - description: List of files based on `config.yaml` - value: list of qc files that will be parsed by multiqc - output: - type: object - description: list of outputs - properties: - html: - type: string - description: MultiQC html report - data: - type: string - description: Data directory with the underlying data found in the html report - - peddy: - type: object - description: input and output parameters for peddy - properties: - input: - type: object - description: list of inputs - properties: - vcf: - type: string - description: Jointly called vcf file from for example glnexus - tbi: - type: string - description: tabix vcf index file - ped: - type: string - description: Pedigree file - output: - type: object - description: list of outputs - properties: - het_check: - type: string - description: General variant QC - pca: - type: string - description: PCA data with ancestry data in .json format - ped: - type: string - description: Calculated pedigree file - ped_check: - type: string - description: Relatedness info - ped_html: - type: string - description: Peddy QC html report - ped_vs_html: - type: string - description: Relatedness compared to input pedigree file reported in html-format - sex_check: - type: string - description: Sex info - - picard_collect_alignment_summary_metrics: - type: object - description: input and output parameters for picard_collect_alignment_summary_metrics - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - bai: - type: string - description: Bam index file - ref: - type: string - description: fasta reference file - output: - type: object - description: list of outputs - properties: - metrics: - type: string - description: Alignment statistics used by MultiQC - - picard_collect_duplication_metrics: - type: object - description: input and output parameters for picard_collect_duplication_metrics - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - bai: - type: string - description: Bam index file - output: - type: object - description: list of outputs - properties: - metrics: - type: string - description: Read duplication statistics used by MultiQC - - picard_collect_gc_bias_metrics: - type: object - description: input and output parameters for picard_collect_gc_bias_metrics - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - bai: - type: string - description: Bam index file - ref: - type: string - description: fasta reference file - output: - type: object - description: list of outputs - properties: - chart: - type: string - description: GC bias figure in pdf format - metrics: - type: string - description: Detailed GC bias statistics - summary: - type: string - description: Summary GC bias statistics - - picard_collect_hs_metrics: - type: object - description: input and output parameters for picard_collect_hs_metrics - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - bai: - type: string - description: Bam index file - bait_intervals: - type: string - description: | - Picard style interval_list file with bait or target regions - NOTE: Can be same file as target_intervals if bait information is missing - reference: - type: string - description: fasta reference file - target_intervals: - type: string - description: Picard style interval_list file with target regions - output: - type: object - description: list of outputs - properties: - metrics: - type: string - description: Panel specific statistics used by MultiQC - - picard_collect_insert_size_metrics: - type: object - description: input and output parameters for picard_collect_insert_size_metrics - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - output: - type: object - description: list of outputs - properties: - pdf: - type: string - description: Pdf with a insert size histogram - txt: - type: string - description: Insert size statistics used by MultiQC - - picard_collect_multiple_metrics: - type: object - description: input and output parameters for picard_collect_multiple_metrics - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - bai: - type: string - description: Bam index file - ref: - type: string - description: fasta reference file - output: - type: object - description: list of outputs - properties: - metrices: - type: string - description: List of statistics analysis files used by MultiQC - - picard_collect_wgs_metrics: - type: object - description: input and output parameters for picard_collect_wgs_metrics - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - bai: - type: string - description: Bam index file - ref: - type: string - description: fasta reference file - interval: - type: string - description: Picard style interval_list file with regions to be analyzed - output: - type: object - description: list of outputs - properties: - metrics: - type: string - description: WGS statistics used by MultiQC - - samtools_idxstats: - type: object - description: input and output parameters for samtools_idxstats - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Aligned bam file - bai: - type: string - description: Bam index file - output: - type: object - description: list of outputs - properties: - stats: - type: string - description: Alignment summary statistics from samtools idxstats - - samtools_stats: - type: object - description: input and output parameters for samtools_stats - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Aligned bam file - output: - type: object - description: list of outputs - properties: - stats: - type: string - description: Alignment summary statistics from samtools stats - - verifybamid2: - type: object - description: input and output parameters for verifybamid2 - properties: - input: - type: object - description: list of inputs - properties: - bam: - type: string - description: Alignment bam file - ref: - type: string - description: Fasta reference file - svd_mu: - type: string - description: Resource file for verifybamid2 - output: - type: object - description: list of outputs - properties: - selfsm: - type: string - description: Text file with the estimated contamination level - ancestry: - type: string - description: Text file containing the PC coordinates for both intended sample and contaminating sample - - - - -