diff --git a/README.md b/README.md index 1f332a9..f2bba69 100644 --- a/README.md +++ b/README.md @@ -120,10 +120,12 @@ The kmer distribution file is generated using the following command line: ## Step 2: Run Kraken 1.0 or Kraken 2.0 AND Generate a report file Kraken 1.0 requires a 2-step process to generate the report file needed by Bracken + kraken --db ${KRAKEN_DB} --threads ${THREADS} ${SAMPLE}.fq > ${SAMPLE}.kraken kraken-report --db ${KRAKEN_DB} ${SAMPLE}.kraken > ${SAMPLE}.kreport Kraken 2.0 requires the addition of the --report flag + kraken2 --db ${KRAKEN_DB} --threads ${THREADS} --report ${SAMPLE}.kreport ${SAMPLE}.fq > ${SAMPLE}.kraken ## Step 3: Run Bracken for Abundance Estimation