diff --git a/README.md b/README.md index 33bd8cf..c6342ed 100644 --- a/README.md +++ b/README.md @@ -12,7 +12,7 @@ ## About -TaxTriage a flexible, containerized bioinformatics pipeline for identification of pathogens within short- or long-read metagenomic sequence data, generated from complex samples/specimens (e.g., respiratory swabs, lesion swabs, whole blood). The workflow includes various software packages to perform quality control, classification, read mapping, as well as generation of confidence metrics and a final report listing anticipated pathogens of relevance. +TaxTriage a flexible, containerized bioinformatics pipeline for identification of pathogens within short- (Illumina) or long-read (ONT, PacBio) metagenomic sequence data, generated from complex samples/specimens (e.g., respiratory swabs, lesion swabs, whole blood). The workflow includes various software packages to perform quality control, classification, read mapping, as well as generation of confidence metrics and a final report listing anticipated pathogens of relevance. ![](assets/taxtriage_schematics.png) diff --git a/conf/modules.config b/conf/modules.config index 1805ae1..6b87a96 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -88,6 +88,10 @@ process { ext.singularity_pull_docker_container = true errorStrategy = 'ignore' } + withName: MAP_TAXID_ASSEMBLY { + ext.singularity_pull_docker_container = true + errorStrategy = 'ignore' + } withName: ORGANISM_MERGE_REPORT { ext.singularity_pull_docker_container = true errorStrategy = 'ignore' diff --git a/docs/usage.md b/docs/usage.md index 43c9d41..a93e25a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -76,7 +76,7 @@ Unless you are using Seqera, most of the temporary directories and final outputs | Column | Description | | -------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | **MANDATORY** Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `platform` | **MANDATORY** Platform used, [ILLUMINA, OXFORD]. If omitted or left blank, assumes ILLUMINA | +| `platform` | **MANDATORY** Platform used, [ILLUMINA, OXFORD]. If omitted or left blank, assumes ILLUMINA. If using PacBio, specify OXFORD. An additional update in the near future will allow PACBIO as an input but has not yet been integrated. | | `fastq_1` | **MANDATORY** Full path to FastQ file for Illumina short reads 1 OR OXFORD reads. File MUST be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `fastq_2` | OPTIONAL Full path to FastQ file for Illumina short reads 2. File MUST be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `trim` | OPTIONAL TRUE/FALSE, do you want to run trimming on the sample? |