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QIIME-Compatible Databases

Primer Information

16S ITS2 ITS1 acdS nifH AMF 18S nosZ
V4_515F_New: 5'-GTGYCAGCMGCCGCGGTAA
V4_806R_New: 5'-GGACTACNVGGGTWTCTAAT		 ITS3: 5'-GCATCGATGAAGAACGCAGC
ITS4: 5'-TCCTCCGCTTATTGATATGC		 ITS1: 5'-GAACCWGCGGARGGATCA
ITS2: 5'-GCTGCGTTCTTCATCGATGC		 acdSF5: 5'-GGCAACAAGMYSCGCAAGCT
acdSR8: 5'-CTGCACSAGSACGCACTTCA		 nifH2: 5'-TGYGAYCCNAARGCNGA
nifH1: 5'-ADNGCCATCATYTCNCC		 AMDGR_NS31: 5'-TTGGAGGGCAAGTCTGGTGCC
AMDGR: 5'-CCCAACTATCCCTATTAATCAT		 nosZ1mod_f: 5'-WCSYTSTTCMTSGAYAGCCAG
nosZmod_R: 5'-ATRTCGATSARCTGVKCRTTYTC

18S QIIME-Compatible Database

The 18S QIIME-compatible database is designed for analyzing eukaryotic microbial communities using the 18S ribosomal RNA (rRNA) gene as a molecular marker. The database includes:

  • reads.qza: Processed 18S rRNA gene sequence reads
  • taxa.qza: Taxonomic annotations linked to the reads
  • classifier.qza: Trained classifier for accurate taxonomic classification

Download 18S Database | Alternative Link

16S QIIME-Compatible Database

The 16S QIIME-compatible database is tailored for exploring bacterial and archaeal communities, utilizing the 16S ribosomal RNA (rRNA) gene as a marker. The database includes:

  • reads.qza: Processed sequence reads
  • taxa.qza: Taxonomic annotations for the reads
  • classifier.qza: Trained classifier for precise taxonomic assignments

Download 16S Database | Alternative Link

ITS QIIME-Compatible Database

The ITS QIIME-compatible database is developed for analyzing fungal communities using the Internal Transcribed Spacer (ITS) region as a gene marker. The database includes:

UNITE-V8

  • reads.qza: Processed ITS sequence reads
  • taxa.qza: Taxonomic information associated with the reads
  • classifier.qza: Trained classifier for accurate taxonomic classification

Download UNITE-V8 ITS Database | Alternative Link

UNITE-V9

  • reads.qza: Processed ITS sequence reads
  • taxa.qza: Taxonomic information associated with the reads
  • classifier.qza: Trained classifier for accurate taxonomic classification

Download UNITE-V9 ITS Database | Alternative Link

nifH QIIME-Compatible Database

The nifH QIIME-compatible database focuses on nitrogen-fixing bacteria, utilizing the nitrogenase reductase (nifH) gene as a marker. The database includes:

  • reads.qza: Processed nifH gene sequence reads
  • taxa.qza: Taxonomic annotations for the reads
  • classifier.qza: Trained classifier for precise taxonomic assignments

Download nifH Database | Alternative Link

acdS QIIME-Compatible Database

The acdS QIIME-compatible database is customized for studying bacterial communities involved in the biogeochemical cycling of ethylene, employing the 1-aminocyclopropane-1-carboxylate deaminase (acdS) gene as a marker. The database includes:

  • reads.qza: Processed acdS gene sequence reads
  • taxa.qza: Taxonomic annotations for the reads
  • classifier.qza: Trained classifier for accurate taxonomic classification

Download acdS Database | Alternative Link

Eukaryota QIIME-Compatible Database

The Eukaryota QIIME-compatible database is tailored for analyzing eukaryotic communities, utilizing various gene markers such as ITS & 18S rRNA. The database includes:

  • reads.qza: Processed sequence reads for eukaryotic genes
  • taxa.qza: Taxonomic annotations associated with the sequence reads
  • classifier.qza: Trained classifier for precise taxonomic classification of eukaryotic sequences

Download Eukaryota Database | Alternative Link

nosZ QIIME-Compatible Database

The nosZ QIIME-compatible database focuses on denitrifying bacteria, utilizing the nitrous oxide reductase (nosZ) gene as a marker. The database includes:

  • reads.qza: Processed nosZ gene sequence reads
  • taxa.qza: Taxonomic annotations for the reads
  • classifier.qza: Trained classifier for precise taxonomic assignments

Download nosZ Database | Alternative Link

QIIME2-Compatible-library by Mitra & Marjan Ghotbi is licensed under Attribution 4.0 International