diff --git a/pkg/capture/outputlocation/blob.go b/pkg/capture/outputlocation/blob.go
index 0367bff4b51..fb58b085a1c 100644
--- a/pkg/capture/outputlocation/blob.go
+++ b/pkg/capture/outputlocation/blob.go
@@ -34,7 +34,7 @@ func (bu *BlobUpload) Name() string {
 }
 
 func (bu *BlobUpload) Enabled() bool {
-	_, err := readBlobURL()
+	_, err := readBlobSASURL()
 	if err != nil {
 		bu.l.Debug("Output location is not enabled", zap.String("location", bu.Name()))
 		return false
@@ -44,13 +44,13 @@ func (bu *BlobUpload) Enabled() bool {
 
 func (bu *BlobUpload) Output(srcFilePath string) error {
 	bu.l.Info("Upload capture file to blob.", zap.String("location", bu.Name()))
-	blobURL, err := readBlobURL()
+	blobURL, err := readBlobSASURL()
 	if err != nil {
 		bu.l.Error("Failed to read blob url", zap.Error(err))
 		return err
 	}
 
-	if err = validateBlobURL(blobURL); err != nil {
+	if err = validateBlobSASURL(blobURL); err != nil {
 		bu.l.Error("Failed to validate blob url", zap.Error(err))
 		return err
 	}
@@ -85,7 +85,18 @@ func (bu *BlobUpload) Output(srcFilePath string) error {
 	return nil
 }
 
-func readBlobURL() (string, error) {
+func trimBlobSASURL(blobSASURL string) string {
+	// Blob SAS URL from the secret created from a file can have a newline and is surrounded by double quotes,
+	// so we need to trim \" and \n and trimming spaces is for unexpected spaces in the URL by customers.
+	// For example:
+	// "\"https://$storage-account-url/$container-name?$blob-sas-token\"\n"
+	trimedSecret := strings.Trim(blobSASURL, "\"\n")
+	trimedSecret = strings.TrimSpace(trimedSecret)
+
+	return trimedSecret
+}
+
+func readBlobSASURL() (string, error) {
 	secretPath := filepath.Join(captureConstants.CaptureOutputLocationBlobUploadSecretPath, captureConstants.CaptureOutputLocationBlobUploadSecretKey)
 	if runtime.GOOS == "windows" {
 		containerSandboxMountPoint := os.Getenv(captureConstants.ContainerSandboxMountPointEnvKey)
@@ -95,11 +106,15 @@ func readBlobURL() (string, error) {
 		secretPath = filepath.Join(containerSandboxMountPoint, captureConstants.CaptureOutputLocationBlobUploadSecretPath, captureConstants.CaptureOutputLocationBlobUploadSecretKey)
 	}
 	secretBytes, err := os.ReadFile(secretPath)
-	return string(secretBytes), err
+	if err != nil {
+		return "", fmt.Errorf("failed to read file %s: %w", secretPath, err)
+	}
+	secretStr := string(secretBytes)
+	return trimBlobSASURL(secretStr), nil
 }
 
-func validateBlobURL(blobURL string) error {
-	u, err := url.Parse(blobURL)
+func validateBlobSASURL(blobSASURL string) error {
+	u, err := url.Parse(blobSASURL)
 	if err != nil {
 		return err
 	}
@@ -107,7 +122,7 @@ func validateBlobURL(blobURL string) error {
 	// Split the path into storage account container and blob
 	path := strings.TrimPrefix(u.Path, "/")
 	if path == "" {
-		return fmt.Errorf("invalid blob URL")
+		return fmt.Errorf("invalid blob SAS URL") //nolint:goerr113
 	}
 
 	return nil
diff --git a/pkg/capture/outputlocation/blob_test.go b/pkg/capture/outputlocation/blob_test.go
index ce8f982c80f..622eacf65cc 100644
--- a/pkg/capture/outputlocation/blob_test.go
+++ b/pkg/capture/outputlocation/blob_test.go
@@ -9,7 +9,45 @@ import (
 	"testing"
 )
 
-func TestValidateBlobURL(t *testing.T) {
+func TestTrimBlobSASURL(t *testing.T) {
+	tests := []struct {
+		name               string
+		inputURL           string
+		expectedTrimmedURL string
+	}{
+		{
+			name:               "valid input URL with sas token that have a newline and is surrounded by double quotes",
+			inputURL:           "\"https://retina.blob.core.windows.net/container/blob?sas-token\"\n",
+			expectedTrimmedURL: "https://retina.blob.core.windows.net/container/blob?sas-token",
+		},
+		{
+			name:               "valid input URL with sas token that have a newline and is surrounded by double quotes and extra spaces",
+			inputURL:           "\"https://retina.blob.core.windows.net/container/blob?sas-token  \"\n",
+			expectedTrimmedURL: "https://retina.blob.core.windows.net/container/blob?sas-token",
+		},
+		{
+			name:               "valid input URL with sas token that has extra spaces",
+			inputURL:           "https://retina.blob.core.windows.net/container/blob?sas-token  ",
+			expectedTrimmedURL: "https://retina.blob.core.windows.net/container/blob?sas-token",
+		},
+		{
+			name:               "valid input URL with sas token",
+			inputURL:           "\"https://retina.blob.core.windows.net/container/blob?sas-token\"\n",
+			expectedTrimmedURL: "https://retina.blob.core.windows.net/container/blob?sas-token",
+		},
+	}
+
+	for _, tt := range tests {
+		t.Run(tt.name, func(t *testing.T) {
+			actualTrimmedBlobSASURL := trimBlobSASURL(tt.inputURL)
+			if actualTrimmedBlobSASURL != tt.expectedTrimmedURL {
+				t.Errorf("Expected trimmed Blob SAS URL %s, but got %s", tt.expectedTrimmedURL, actualTrimmedBlobSASURL)
+			}
+		})
+	}
+}
+
+func TestValidateBlobSASURL(t *testing.T) {
 	tests := []struct {
 		name          string
 		inputURL      string
@@ -17,7 +55,7 @@ func TestValidateBlobURL(t *testing.T) {
 	}{
 		{
 			name:          "valid input URL with sas token",
-			inputURL:      "https://retina.blob.core.windows.net/container/blob?sp=r&st=2023-02-17T19:13:30Z&se=2023-02-18T03:13:30Z&spr=https&sv=2021-06-08&sr=c&sig=NtSxlRK5Vs4kVs1dIOfr%2FMdLKBVTA4t3uJ0gqLZ9exk%3D",
+			inputURL:      "https://retina.blob.core.windows.net/container/blob?sas-token",
 			expectedError: nil,
 		},
 		{
@@ -44,7 +82,7 @@ func TestValidateBlobURL(t *testing.T) {
 
 	for _, tt := range tests {
 		t.Run(tt.name, func(t *testing.T) {
-			err := validateBlobURL(tt.inputURL)
+			err := validateBlobSASURL(tt.inputURL)
 
 			if err != nil && tt.expectedError == nil {
 				t.Errorf("Unexpected error: %v", err)