diff --git a/.github/workflows/api-tests.yml b/.github/workflows/api-tests.yml
index e1c0bf33a4ff31..2cd0b2a7d430de 100644
--- a/.github/workflows/api-tests.yml
+++ b/.github/workflows/api-tests.yml
@@ -50,6 +50,9 @@ jobs:
       - name: Run ModelRuntime
         run: poetry run -C api bash dev/pytest/pytest_model_runtime.sh
 
+      - name: Run dify config tests
+        run: poetry run -C api python dev/pytest/pytest_config_tests.py
+
       - name: Run Tool
         run: poetry run -C api bash dev/pytest/pytest_tools.sh
 
diff --git a/dev/pytest/pytest_config_tests.py b/dev/pytest/pytest_config_tests.py
new file mode 100644
index 00000000000000..08adc9ebe999b1
--- /dev/null
+++ b/dev/pytest/pytest_config_tests.py
@@ -0,0 +1,111 @@
+import yaml  # type: ignore
+from dotenv import dotenv_values
+from pathlib import Path
+
+BASE_API_AND_DOCKER_CONFIG_SET_DIFF = {
+    "APP_MAX_EXECUTION_TIME",
+    "BATCH_UPLOAD_LIMIT",
+    "CELERY_BEAT_SCHEDULER_TIME",
+    "CODE_EXECUTION_API_KEY",
+    "HTTP_REQUEST_MAX_CONNECT_TIMEOUT",
+    "HTTP_REQUEST_MAX_READ_TIMEOUT",
+    "HTTP_REQUEST_MAX_WRITE_TIMEOUT",
+    "KEYWORD_DATA_SOURCE_TYPE",
+    "LOGIN_LOCKOUT_DURATION",
+    "LOG_FORMAT",
+    "OCI_ACCESS_KEY",
+    "OCI_BUCKET_NAME",
+    "OCI_ENDPOINT",
+    "OCI_REGION",
+    "OCI_SECRET_KEY",
+    "REDIS_DB",
+    "RESEND_API_URL",
+    "RESPECT_XFORWARD_HEADERS_ENABLED",
+    "SENTRY_DSN",
+    "SSRF_DEFAULT_CONNECT_TIME_OUT",
+    "SSRF_DEFAULT_MAX_RETRIES",
+    "SSRF_DEFAULT_READ_TIME_OUT",
+    "SSRF_DEFAULT_TIME_OUT",
+    "SSRF_DEFAULT_WRITE_TIME_OUT",
+    "UPSTASH_VECTOR_TOKEN",
+    "UPSTASH_VECTOR_URL",
+    "USING_UGC_INDEX",
+    "WEAVIATE_BATCH_SIZE",
+    "WEAVIATE_GRPC_ENABLED",
+}
+
+BASE_API_AND_DOCKER_COMPOSE_CONFIG_SET_DIFF = {
+    "BATCH_UPLOAD_LIMIT",
+    "CELERY_BEAT_SCHEDULER_TIME",
+    "HTTP_REQUEST_MAX_CONNECT_TIMEOUT",
+    "HTTP_REQUEST_MAX_READ_TIMEOUT",
+    "HTTP_REQUEST_MAX_WRITE_TIMEOUT",
+    "KEYWORD_DATA_SOURCE_TYPE",
+    "LOGIN_LOCKOUT_DURATION",
+    "LOG_FORMAT",
+    "OPENDAL_FS_ROOT",
+    "OPENDAL_S3_ACCESS_KEY_ID",
+    "OPENDAL_S3_BUCKET",
+    "OPENDAL_S3_ENDPOINT",
+    "OPENDAL_S3_REGION",
+    "OPENDAL_S3_ROOT",
+    "OPENDAL_S3_SECRET_ACCESS_KEY",
+    "OPENDAL_S3_SERVER_SIDE_ENCRYPTION",
+    "PGVECTOR_MAX_CONNECTION",
+    "PGVECTOR_MIN_CONNECTION",
+    "PGVECTO_RS_DATABASE",
+    "PGVECTO_RS_HOST",
+    "PGVECTO_RS_PASSWORD",
+    "PGVECTO_RS_PORT",
+    "PGVECTO_RS_USER",
+    "RESPECT_XFORWARD_HEADERS_ENABLED",
+    "SCARF_NO_ANALYTICS",
+    "SSRF_DEFAULT_CONNECT_TIME_OUT",
+    "SSRF_DEFAULT_MAX_RETRIES",
+    "SSRF_DEFAULT_READ_TIME_OUT",
+    "SSRF_DEFAULT_TIME_OUT",
+    "SSRF_DEFAULT_WRITE_TIME_OUT",
+    "STORAGE_OPENDAL_SCHEME",
+    "SUPABASE_API_KEY",
+    "SUPABASE_BUCKET_NAME",
+    "SUPABASE_URL",
+    "USING_UGC_INDEX",
+    "VIKINGDB_CONNECTION_TIMEOUT",
+    "VIKINGDB_SOCKET_TIMEOUT",
+    "WEAVIATE_BATCH_SIZE",
+    "WEAVIATE_GRPC_ENABLED",
+}
+
+API_CONFIG_SET = set(dotenv_values(Path("api") / Path(".env.example")).keys())
+DOCKER_CONFIG_SET = set(dotenv_values(Path("docker") / Path(".env.example")).keys())
+DOCKER_COMPOSE_CONFIG_SET = set()
+
+with open(Path("docker") / Path("docker-compose.yaml")) as f:
+    DOCKER_COMPOSE_CONFIG_SET = set(yaml.safe_load(f.read())["x-shared-env"].keys())
+
+
+def test_yaml_config():
+    # python set == operator is used to compare two sets
+    DIFF_API_WITH_DOCKER = (
+        API_CONFIG_SET - DOCKER_CONFIG_SET - BASE_API_AND_DOCKER_CONFIG_SET_DIFF
+    )
+    if DIFF_API_WITH_DOCKER:
+        print(
+            f"API and Docker config sets are different with key: {DIFF_API_WITH_DOCKER}"
+        )
+        raise Exception("API and Docker config sets are different")
+    DIFF_API_WITH_DOCKER_COMPOSE = (
+        API_CONFIG_SET
+        - DOCKER_COMPOSE_CONFIG_SET
+        - BASE_API_AND_DOCKER_COMPOSE_CONFIG_SET_DIFF
+    )
+    if DIFF_API_WITH_DOCKER_COMPOSE:
+        print(
+            f"API and Docker Compose config sets are different with key: {DIFF_API_WITH_DOCKER_COMPOSE}"
+        )
+        raise Exception("API and Docker Compose config sets are different")
+    print("All tests passed!")
+
+
+if __name__ == "__main__":
+    test_yaml_config()