From aeb5cc04485dc04b6dd4caeab0c1bdd845e3da47 Mon Sep 17 00:00:00 2001 From: SietsmaRJ Date: Thu, 29 Sep 2022 09:39:16 +0200 Subject: [PATCH 1/5] feat: Simplified BCFTools - Simplified the BCFTools conversion script to no longer use a hardcoded header as a requirement, this is now replaced by using the CSQ field together with the -A flag. --- scripts/convert_vep_vcf_to_tsv_capice.sh | 10 +++------- 1 file changed, 3 insertions(+), 7 deletions(-) diff --git a/scripts/convert_vep_vcf_to_tsv_capice.sh b/scripts/convert_vep_vcf_to_tsv_capice.sh index 516c1ea6..59c5d4c0 100755 --- a/scripts/convert_vep_vcf_to_tsv_capice.sh +++ b/scripts/convert_vep_vcf_to_tsv_capice.sh @@ -3,9 +3,6 @@ # Stops script if any error occurs. set -e -# Possibly variable variables -PRE_HEADER="%CHROM\t%POS\t%REF\t%ALT\t%Consequence\t%SYMBOL\t%SYMBOL_SOURCE\t%Gene\t%Feature\t%Feature_type\t%cDNA_position\t%CDS_position\t%Protein_position\t%Amino_acids\t%STRAND\t%SIFT\t%PolyPhen\t%EXON\t%INTRON\t%SpliceAI_pred_DP_AG\t%SpliceAI_pred_DP_AL\t%SpliceAI_pred_DP_DG\t%SpliceAI_pred_DP_DL\t%SpliceAI_pred_DS_AG\t%SpliceAI_pred_DS_AL\t%SpliceAI_pred_DS_DG\t%SpliceAI_pred_DS_DL\t%gnomAD_AF" - # Defines error echo. errcho() { echo "$@" 1>&2; } @@ -125,12 +122,11 @@ processFile() { local output="${output%.gz}" # Strips '.gz' to better work with code below. local output_tmp="${output}.tmp" - local format="${PRE_HEADER}\n" - local args=() args+=("+split-vep") args+=("-d") - args+=("-f" "${format}") + args+=("-f" "%CHROM\t%POS\t%REF\t%ALT\t%CSQ\n") + args+=("-A" "tab") args+=("-o" "${output_tmp}") args+=("${input}") @@ -140,7 +136,7 @@ processFile() { echo "BCFTools finished, building output file." - echo -e "${PRE_HEADER}" | cat - "${output_tmp}" > "${output}" && rm "${output_tmp}" + echo -e "CHROM\tPOS\tREF\tALT\t$(bcftools +split-vep -l ${input} | cut -f 2 | tr '\n' '\t' | sed 's/\t$//')" | cat - "${output_tmp}" > "${output}" && rm "${output_tmp}" echo "Output file ready, gzipping." From 30a7a0656424f21e917416376257d0c82cc59536 Mon Sep 17 00:00:00 2001 From: SietsmaRJ Date: Thu, 29 Sep 2022 10:25:22 +0200 Subject: [PATCH 2/5] - Removed the correction of the % sign --- .../capice/utilities/load_file_postprocessor.py | 15 +-------------- 1 file changed, 1 insertion(+), 14 deletions(-) diff --git a/src/molgenis/capice/utilities/load_file_postprocessor.py b/src/molgenis/capice/utilities/load_file_postprocessor.py index e347f3d2..9f3b5366 100644 --- a/src/molgenis/capice/utilities/load_file_postprocessor.py +++ b/src/molgenis/capice/utilities/load_file_postprocessor.py @@ -20,24 +20,11 @@ def process(self): dataset : pandas.DataFrame Processed dataset with corrected % sign and renamed columns. """ - self.log.debug('Starting correcting % sign.') - self._correct_percentage_sign() - self.log.debug('% sign corrected, starting renaming of columns.') + self.log.info('LoadFilePostProcessor starting.') self._col_renamer() self.log.info('LoadFilePostProcessor successful.') return self.dataset - def _correct_percentage_sign(self): - new_columns = [] - for column in self.dataset.columns: - if column.startswith('%'): - new_columns.append(column.split('%')[1]) - elif column.startswith('#'): - new_columns.append(column.split('#')[1]) - else: - new_columns.append(column) - self.dataset.columns = new_columns - def _col_renamer(self): """ Function to rename "Gene, Feature, SYMBOL, INTRON and EXON" to From 970cfb47d3e2aafb67ec9ff1cfa55fb23e570480 Mon Sep 17 00:00:00 2001 From: SietsmaRJ Date: Thu, 29 Sep 2022 11:02:22 +0200 Subject: [PATCH 3/5] - Added support for the -t flag --- scripts/convert_vep_vcf_to_tsv_capice.sh | 11 +++++++---- 1 file changed, 7 insertions(+), 4 deletions(-) diff --git a/scripts/convert_vep_vcf_to_tsv_capice.sh b/scripts/convert_vep_vcf_to_tsv_capice.sh index 59c5d4c0..959fdab2 100755 --- a/scripts/convert_vep_vcf_to_tsv_capice.sh +++ b/scripts/convert_vep_vcf_to_tsv_capice.sh @@ -54,8 +54,11 @@ digestCommandLine() { if [[ ${TRAIN} == true ]] then - id="\t%ID" - PRE_HEADER="$PRE_HEADER$id" + HEADER="CHROM\tPOS\tID\tREF\tALT\t" + FORMAT="%CHROM\t%POS\t%ID\t%REF\t%ALT\t%CSQ\n" + else + HEADER="CHROM\tPOS\tREF\tALT\t" + FORMAT="%CHROM\t%POS\t%REF\t%ALT\t%CSQ\n" fi validateCommandLine @@ -125,7 +128,7 @@ processFile() { local args=() args+=("+split-vep") args+=("-d") - args+=("-f" "%CHROM\t%POS\t%REF\t%ALT\t%CSQ\n") + args+=("-f" "${FORMAT}") args+=("-A" "tab") args+=("-o" "${output_tmp}") args+=("${input}") @@ -136,7 +139,7 @@ processFile() { echo "BCFTools finished, building output file." - echo -e "CHROM\tPOS\tREF\tALT\t$(bcftools +split-vep -l ${input} | cut -f 2 | tr '\n' '\t' | sed 's/\t$//')" | cat - "${output_tmp}" > "${output}" && rm "${output_tmp}" + echo -e "${HEADER}$(bcftools +split-vep -l "${input}" | cut -f 2 | tr '\n' '\t' | sed 's/\t$//')" | cat - "${output_tmp}" > "${output}" && rm "${output_tmp}" echo "Output file ready, gzipping." From 4fb6ebb90518d407c5f49ba7b0f1204080bf9045 Mon Sep 17 00:00:00 2001 From: SietsmaRJ Date: Thu, 29 Sep 2022 15:02:25 +0200 Subject: [PATCH 4/5] - Updated testing resources --- resources/predict_input.tsv.gz | Bin 466 -> 465 bytes resources/train_input.tsv.gz | Bin 125762 -> 125751 bytes .../utilities/test_file_postprocessor.py | 18 +++++++++--------- .../utilities/test_load_file_postprocessor.py | 4 ++-- tests/resources/breakends_vep.tsv.gz | Bin 431 -> 429 bytes tests/resources/edge_cases_vep.tsv.gz | Bin 556 -> 554 bytes tests/resources/symbolic_alleles_vep.tsv.gz | Bin 568 -> 569 bytes 7 files changed, 11 insertions(+), 11 deletions(-) diff --git a/resources/predict_input.tsv.gz b/resources/predict_input.tsv.gz index 00d9e5fe21b0d10636b11f6174083237ae51c982..450a5a4a573157e3fb5fd9dcfeb10afc66909e7d 100644 GIT binary patch literal 465 zcmV;?0WSU@iwFqSl{I4m|8R0;WNBk`UukY|b#yLtb9Mlvkx_4(FcgKK@BT}`*9IFN zDI{q$0ug5G_GBSqX-KVMKqqy-es+>JslwX?J{OLydp{kgk5##cLn)zJZy?EQNP9Es 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