From 2caee95b7578b82cba5c2e49b353b368b8c87c39 Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 26 Jan 2024 11:48:11 +0100 Subject: [PATCH 01/16] Remove Gnomad_HN --- resources/train_features.json | 3 +-- tests/capice/test_main_train.py | 3 +-- 2 files changed, 2 insertions(+), 4 deletions(-) diff --git a/resources/train_features.json b/resources/train_features.json index a3fa66e5..160a6d1a 100644 --- a/resources/train_features.json +++ b/resources/train_features.json @@ -17,6 +17,5 @@ "SpliceAI_pred_DS_DG": null, "SpliceAI_pred_DS_DL": null, "Grantham": null, - "phyloP": null, - "gnomAD_HN": null + "phyloP": null } \ No newline at end of file diff --git a/tests/capice/test_main_train.py b/tests/capice/test_main_train.py index b651e3e3..5207ad19 100644 --- a/tests/capice/test_main_train.py +++ b/tests/capice/test_main_train.py @@ -106,8 +106,7 @@ def test_integration_reset_train_features(self): 'is_NMD_transcript_variant', 'is_feature_elongation', 'is_feature_truncation', 'SpliceAI_pred_DP_AG', 'SpliceAI_pred_DP_AL', 'SpliceAI_pred_DP_DG', 'SpliceAI_pred_DP_DL', 'SpliceAI_pred_DS_AG', 'SpliceAI_pred_DS_AL', - 'SpliceAI_pred_DS_DG', 'SpliceAI_pred_DS_DL', 'Type', 'Length', 'Grantham', 'phyloP', - 'gnomAD_HN' + 'SpliceAI_pred_DS_DG', 'SpliceAI_pred_DS_DL', 'Type', 'Length', 'Grantham', 'phyloP' ] self.assertSetEqual(set(observed), set(expected)) From 609e74514aa5e4ea38caa10ece9944630a982508 Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 26 Jan 2024 12:55:28 +0100 Subject: [PATCH 02/16] Fix travis --- sonar-project.properties | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/sonar-project.properties b/sonar-project.properties index f526c3bb..94c828bd 100644 --- a/sonar-project.properties +++ b/sonar-project.properties @@ -7,4 +7,5 @@ sonar.tests = tests/ sonar.python.xunit.reportPath=results.xml sonar.python.coverage.reportPaths=coverage.xml -sonar.python.version=3.10 \ No newline at end of file +sonar.python.version=3.10 +sonar.java.version=17.0.10 \ No newline at end of file From 2b11b25482891a983fa4ed4fc57fa1856bcbedb3 Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 26 Jan 2024 13:53:53 +0100 Subject: [PATCH 03/16] try to fix sonar --- sonar-project.properties | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/sonar-project.properties b/sonar-project.properties index 94c828bd..b03bf9d2 100644 --- a/sonar-project.properties +++ b/sonar-project.properties @@ -8,4 +8,5 @@ sonar.python.xunit.reportPath=results.xml sonar.python.coverage.reportPaths=coverage.xml sonar.python.version=3.10 -sonar.java.version=17.0.10 \ No newline at end of file +sonar.java.source=21 +sonar.java.target=21 \ No newline at end of file From e48c278da9ae1777236bedec0630d7051d61a66f Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 26 Jan 2024 13:59:38 +0100 Subject: [PATCH 04/16] try to fix sonar attempt 4 --- .travis.yml | 2 +- sonar-project.properties | 4 +--- 2 files changed, 2 insertions(+), 4 deletions(-) diff --git a/.travis.yml b/.travis.yml index ba215be9..cc367259 100644 --- a/.travis.yml +++ b/.travis.yml @@ -1,5 +1,5 @@ os: linux -dist: focal +dist: jammy language: python python: - '3.10' diff --git a/sonar-project.properties b/sonar-project.properties index b03bf9d2..f526c3bb 100644 --- a/sonar-project.properties +++ b/sonar-project.properties @@ -7,6 +7,4 @@ sonar.tests = tests/ sonar.python.xunit.reportPath=results.xml sonar.python.coverage.reportPaths=coverage.xml -sonar.python.version=3.10 -sonar.java.source=21 -sonar.java.target=21 \ No newline at end of file +sonar.python.version=3.10 \ No newline at end of file From f931e559ff5d8f48c1efde9dbf36f7b6d9bf05cf Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 26 Jan 2024 14:05:29 +0100 Subject: [PATCH 05/16] try to fix sonar attempt 5 --- .travis.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.travis.yml b/.travis.yml index cc367259..4a513a3a 100644 --- a/.travis.yml +++ b/.travis.yml @@ -3,6 +3,7 @@ dist: jammy language: python python: - '3.10' +jdk: openjdk17 cache: pip branches: only: From 54aba9fbad02c52a56c2686022f152c5cac41a0e Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 26 Jan 2024 14:12:00 +0100 Subject: [PATCH 06/16] try to fix sonar attempt 6 --- .travis.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.travis.yml b/.travis.yml index 4a513a3a..5ce79d9d 100644 --- a/.travis.yml +++ b/.travis.yml @@ -19,6 +19,7 @@ script: - sonar-scanner addons: sonarcloud: + jdk: openjdk17 organization: molgenis token: secure: qFkmx02PjcBy6nCpf05evyhQBcwOqq3BHycZbmyYkKE9AS9AbYH7eiGboTOvO5N45d3UoZaUud22JK+rD4mKTm9fMzhxQLrexww+EUOCs0EYRDaQkBtl9HcgnDWnHtRl55151agb8VJ2l9AFMC6vlfJjs+nYGeFimhoat0s4NxWlhQV/fEdmmlJCj9k37Z1We0SSoSuw8A+kJ5S3Lzf+7mhm2o/BF1rvjipW9m66e6uOyNmm9n2FMi9em06OdQRDoeLIXjgqvF2SvphcOwXfiL3TFJR4R3wjajRqgGlRmCcdt/HSMe5eTjcNvHiE3HmeAKSLoItixSqxBxzLrW/lBziWPp6E4GqRpyePhprUzJDIeB4nPg28CHS5mbqtsEiJFkGKwcaDWd+jY/KHIhN/5ECMGGQrBsiB0v7ENwasa46pMzsZ9m9b1oZzMO+dKxBl9N7BGmcjZp37nRv7n7zo5SGn1NMSs+w+8C542mAuVT0TCemKf1178auK2zeHguUxt+/GHw67lGyegZC17a9DAVN4IBCU+hkrFSwhfk1VqXulVM/b+TDpHggOaRLTNR5u45j+ibNTASTvTHwVDlbaGOOrcBSjoMY8yf+/777g3KzQFofXyyaiWRcVQAg1pl8XJUVNSJkULxCZlxy9CXc0dmnwRpui6fGtHgNpn/hwXvk= From c2dc9431063cb6788d03be9cf40b7bf1d49b1af1 Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 26 Jan 2024 14:21:28 +0100 Subject: [PATCH 07/16] try to fix sonar attempt 7 --- .travis.yml | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/.travis.yml b/.travis.yml index 5ce79d9d..07efedd7 100644 --- a/.travis.yml +++ b/.travis.yml @@ -1,6 +1,6 @@ os: linux dist: jammy -language: python +language: java python: - '3.10' jdk: openjdk17 @@ -19,7 +19,6 @@ script: - sonar-scanner addons: sonarcloud: - jdk: openjdk17 organization: molgenis token: secure: 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 From 61b9cd48d99c51dd61a27c3085cca53b1057f3bf Mon 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za^+A%KsluI<#*eOYfC{$GY>yC=8#!z5C``2e?9f>f)!&qK`<}VK4I*hz0e*t(u<1|0l|$BV<0m?D zIr7;=5v?Smxo9Z->HEWv{lIHMQ4=#N>Ids21WD0e5Yq1l4tg>JuP?;VC0`@wo=?Az zJ{XlqNrel-MGs+}8U1@qSy+8jP%?D~gI~2PkE}#w|sy zAIg>#bm@ML@LK7g{X%-y4}{F>wX!7JZ6X6|v`V712>Q$a#nsC2heZ9HxsVzD!2h4C zo1O9h^38i%ET4Zw@3#f_s2Dr-$mzF8D+gu=A$7?xsg)d;{cDe(NA<47D0rQ<9}=&fi%xR^2y3-lmyMdC}(ZcWt_PEo4oxepj1_ z#jGR=@!OT#9a~%@URSOs)sO2XpC7+3U*_ZAi=(Qs{79ZcL?@#mLoX7KwbzfYWq{Oq zf6951+e4ifh>+@txFTD}qeqQ%uhT*YWqnw^jsH13lIh|a1{T7ZgI#jNbp{q0W{tL& z;Hj_Dd%Qd!k1I7Z47P&4Y9|xex$gp!GR7jyhi>Lj6I;CI Date: Fri, 26 Jan 2024 14:51:22 +0100 Subject: [PATCH 09/16] Update VEP cmd in readme --- README.md | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/README.md b/README.md index 9b955f70..8e2dcd13 100644 --- a/README.md +++ b/README.md @@ -99,11 +99,10 @@ vep --input_file --format vcf --output_file --dont_skip --allow_non_variant --use_given_ref --exclude_predicted \ --flag_pick_allele --plugin Grantham \ --plugin SpliceAI,snv=,indel= \ ---custom ",gnomAD,vcf,exact,0,AF,HN" \ ---custom ",phyloP,bigwig,exact,0" \ +--custom ",gnomAD,vcf,exact,0,AF" \ +--custom " ``` -**IMPORTANT: Ensure the right files are used based on GRCH37 or GRCH38!!!** Note: Certain arguments might not be needed if training/predicting without using all possible features offered by CAPICE. From 27ee59df1a36045e79f6e7c72c46eccb8cee90e7 Mon Sep 17 00:00:00 2001 From: "bart.charbon" Date: Fri, 2 Feb 2024 11:24:25 +0100 Subject: [PATCH 10/16] Build38 testdata --- resources/breakends_grch38.vcf.gz | Bin 2245 -> 0 bytes resources/edge_cases_grch38.vcf.gz | Bin 2362 -> 0 bytes resources/predict_input.tsv.gz | Bin 758 -> 0 bytes resources/predict_input_raw.vcf.gz | Bin 675 -> 0 bytes resources/predict_input_raw_grch38.vcf.gz | Bin 2299 -> 0 bytes resources/symbolic_alleles_grch38.vcf.gz | Bin 2890 -> 0 bytes resources/test_input.vcf | 22 +++++++++++----------- resources/train_input.tsv.gz | Bin 150137 -> 0 bytes resources/train_input_raw.vcf.gz | Bin 33940 -> 0 bytes resources/train_input_raw_grch38.vcf.gz | Bin 40434 -> 0 bytes tests/resources/breakends.vcf.gz | Bin 447 -> 2245 bytes tests/resources/edge_cases.vcf.gz | Bin 790 -> 2362 bytes tests/resources/symbolic_alleles.vcf.gz | Bin 961 -> 2890 bytes 13 files changed, 11 insertions(+), 11 deletions(-) delete mode 100644 resources/breakends_grch38.vcf.gz delete mode 100644 resources/edge_cases_grch38.vcf.gz delete mode 100644 resources/predict_input.tsv.gz delete mode 100644 resources/predict_input_raw_grch38.vcf.gz delete mode 100644 resources/symbolic_alleles_grch38.vcf.gz delete mode 100644 resources/train_input.tsv.gz delete mode 100644 resources/train_input_raw_grch38.vcf.gz mode change 100755 => 100644 tests/resources/breakends.vcf.gz mode change 100755 => 100644 tests/resources/edge_cases.vcf.gz mode change 100755 => 100644 tests/resources/symbolic_alleles.vcf.gz diff --git a/resources/breakends_grch38.vcf.gz b/resources/breakends_grch38.vcf.gz deleted file mode 100644 index 9856f8e2e0b7200992c659763d0a5382cd9eee85..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2245 zcmV;$2s-y4iwFoAeX?Z$17dPzVQXb>WOH9VH|4r5>+0l(`{yFNJ-^8E>M?8K|EJgY zS-zLep7WO?dnk%3+w2#}=4geitg>bK)YkA()|Az}*eO`$>vdTzvZqbG+C5le&-o{kkwC}mhHUVZt`XJl5fhqYQIzXH(31t4p_5SI*8HZcIWy0IjE+$* z{$_T2{^k7k?mS!Pn|xKY;MmU4E`i$yTz=ekx!8$ZgX=e)>)`eSkrc@{QD#18e&k*AIjsZd_G>(FHga?ag5OijEq4= zYb}8>2_uv=02*U#j|tN1peQ}A$9NHhjh?{x9%G|6fYD=2=rP7h9h`P!Ozbf_=xDt3 zV~i9Dqc5T#5YD}r+*NFhziTP+CJWQw&k+8M0^0ZPFZUKs5q1xnEtGDax_wOk+W zC^Q7Y5LuIbxPw;K8zTu!AMPNu2cs!HeYkCK(mP3HMD@{j27aU?GNSrm8|Ougfyln< zV-4w$!8js7|4A%z!U(94G3INp#w+EGBp|=_YMgach-bfEDGzyaFLKtk^?H>kZUt2* zy@(%)XeBhot%F1uFQU8)oJbsrI2W9FTSAJFgb|QXMnaAyl#<*Uiij4ZiO6*?q6xD+ z$>@WG8B0jA3#fyH9h>DObPh&@$h(D3LiO3%f#(XqFID%Zg#CQ!2UEMGeV>&|O1C_>uh#K=zM`0;;9WqZvSO`tE zB-w{J5+Rmkhz~MKjrpRal+=a=GOxA~)Q^Vt=XD+UoRE=*>_`1N<9g9SQY|itjF-`o z-0G7EQ88Wxvi5tKamm0YrzvObL4=^D2{89id)`5YXqEvqQDcephpMq77RUe-5w$7$ z!$MuDAv{%XNoLG8*ygA{n?%OMkuVu~YP#%Y2!c9G zk~+zZsbhdSYy^mA8)Q`MGGppU0kaT6og_o_&Zs?62uWm20(eFZN?%_k4AIx11PNdM zsP-aSKsI}d!N9^n4T3^0IH*ogXf;Hh6VxK8p(xQq6}A@ENQLA!coQ3@&lO?90|*9$Gd60S^X-By_iu928X-%*mVq0p-g{%W*5Be8B+7RaZojh1hWe4<B8BHOI9j&?2dyuGBDeb#$Btswd1s6 zW=U?2)Dd$a1TI*(xPby3T_Q?s^sG|6z7K4y@Dasu)2SY82A(?EC#@K&1!n;6nb2HV zTk*k*x{^&wu~(5LcEF=4ck0r(WgEo}kZ^v(T%le~B`7XeXIZEkgo>@f!N$$an%d)& zFBC5a#fQnj4(52s@G)}ph{Aikd*k-u^%`_=mQvx3ZEJs%H<)&PlyCHm*Nfc29tSO! zF+<_gCunxu6z2`*qVpxtlC+@em3n!-c}4+-c_($7AboMO&^_dW&-B;S5jP@Ji#s9O%h+ z-6?mt1qMgHGf0}ttm#4K?B%a^1N`p+(0-4~e7^0w$+76~}DN_f_P z_2s1d^uu(qskiH9x?Rl|KQ89;X}ekrHGL_&Z+E-RmXC0=OA5Ukyw^4B3 zvd7ZS_RnWkJ-+QDN%tL_ci@1U=pEYe}L+WLH#*#lh3Djca#11 TkWPO2boTCld30(FpCteQoHkfK diff --git a/resources/edge_cases_grch38.vcf.gz b/resources/edge_cases_grch38.vcf.gz deleted file mode 100644 index c92b95e60f00869a89aee56835df5bf880a8b776..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2362 zcmV-A3B~pwiwFoKfU;!(17&1qWnW`qb7ga1XL4g`GdM1GV`eUAdM4A72*B>?JQ&Y1x0eynZw1>chv6gWGZc)99*yHM~8$%C^&Du_+h%y8p{n zw#=%(e)$cdMt4tb)M)*d726Z+`u^eb;HdA*+1+mSoE3d}HvYcJ`lEH3&9kB{KGAHkf zRKVwKmMqrr#bOJKo94x=ZRjDZVwYX!tIaZ7Wng4BSS}&#+mn59uz$Tw-+4X;V-LgY zBn2lb!;qgOJ;)}n>06dOXW1Hz?hPbUWTEnCxp*ma_$t{h*3)dSV3lq*5TE3w$XELZ z8xUUPyUOzyi0~el@4uPu3iwbd7O;zClAA?;ltxF*`!6f%Aht6j#fO`ZDGl?U6qTy$So}WzFImg z`x$7nE3yM=run*D%=h7+z83QAK!Uz9AzEjY>S9!bQ8H?)Vi%)N7#V|#)>;B%8jMiV z0H}+xH6}=_gQE1f8skL}HhKc%YmANB07iE)p~e_1b#U5sF|o$zpri596EPGGNc-r? 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zXwdLMqsNAY729Tuupxn?g9eQo8>aluvT?67iuyIhtqltvJEmWKbt_8+mk2fuHkt8% E09F(awEzGB From 4a229356bfb957c9d02e809ae23e170d5c3c5cf0 Mon Sep 17 00:00:00 2001 From: Bart Charbon Date: Tue, 6 Feb 2024 16:25:16 +0100 Subject: [PATCH 15/16] Version bump --- src/molgenis/capice/__init__.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/molgenis/capice/__init__.py b/src/molgenis/capice/__init__.py index 40f5033b..deda00ee 100644 --- a/src/molgenis/capice/__init__.py +++ b/src/molgenis/capice/__init__.py @@ -1 +1 @@ -__version__ = '5.1.1' +__version__ = '5.1.2' From 2f6c447387319b819cac398ffbde474ccb947f23 Mon Sep 17 00:00:00 2001 From: Bart Charbon Date: Tue, 6 Feb 2024 16:37:36 +0100 Subject: [PATCH 16/16] Remove GRCh37 references --- README.md | 18 ++---------------- 1 file changed, 2 insertions(+), 16 deletions(-) diff --git a/README.md b/README.md index 8e2dcd13..4ac93426 100644 --- a/README.md +++ b/README.md @@ -14,24 +14,12 @@ below. CAPICE can be used as online service at http://molgenis.org/capice ## Requirements -The list below is a complete list. Depending on whether GRCh37 and/or GRCh38 is used and whether all -mentioned features are used, some items in the list below can be skipped. - * VEP v107 * Including VEP cache (which needs to be unarchived!): - * [homo_sapiens_refseq_vep_107_GRCh37](http://ftp.ensembl.org/pub/release-107/variation/indexed_vep_cache/homo_sapiens_refseq_vep_107_GRCh37.tar.gz) * [homo_sapiens_refseq_vep_107_GRCh38](http://ftp.ensembl.org/pub/release-107/variation/indexed_vep_cache/homo_sapiens_refseq_vep_107_GRCh38.tar.gz) * Including plugin(s): * [Grantham](https://github.com/molgenis/vip/blob/master/resources/vep/plugins/Grantham.pm) * [SpliceAI](https://github.com/molgenis/vip/blob/master/resources/vep/plugins/SpliceAI.pm) - * Including additional data (GRCh37) [available here](https://download.molgeniscloud.org/downloads/vip/resources/GRCh37/): - * `gnomad.total.r2.1.1.sites.stripped.vcf.gz` - * `gnomad.total.r2.1.1.sites.stripped.vcf.gz.csi` - * `hg19.100way.phyloP100way.bw` - * `spliceai_scores.masked.indel.hg19.vcf.gz` - * `spliceai_scores.masked.indel.hg19.vcf.gz.tbi` - * `spliceai_scores.masked.snv.hg19.vcf.gz` - * `spliceai_scores.masked.snv.hg19.vcf.gz.tbi` * Including additional data (GRCh38) [available here](https://download.molgeniscloud.org/downloads/vip/resources/GRCh38/): * `gnomad.genomes.v3.1.2.sites.stripped.vcf.gz` * `gnomad.genomes.v3.1.2.sites.stripped.vcf.gz.csi` @@ -95,7 +83,7 @@ command: vep --input_file --format vcf --output_file \ --vcf --compress_output gzip --sift s --polyphen s --numbers --symbol \ --shift_3prime 1 --allele_number --refseq --total_length --no_stats --offline --cache \ ---dir_cache --species "homo_sapiens" --assembly \ +--dir_cache --species "homo_sapiens" --assembly \ --fork --dont_skip --allow_non_variant --use_given_ref --exclude_predicted \ --flag_pick_allele --plugin Grantham \ --plugin SpliceAI,snv=,indel= \ @@ -332,19 +320,17 @@ To convert a `.pickle.dat` model to `.ubj`/`.json`, one can do the following (us - `sys.argv[1]`: the input `.pickle.dat` file - `sys.argv[2]`: the output `.json` file 4. Adjust `CAPICE_version` within the new model file to that of the new release (major version should match!). - It is advisable however to ensure that the filename still contains the version it was trained on (f.e. `v4.0.0_grch37_v5.0.0-compatibility.json`) to ensure no confusion will exist on which version it was actually trained. + It is advisable however to ensure that the filename still contains the version it was trained on (e.g. `v4.0.0_grch38_v5.0.0-compatibility.json`) to ensure no confusion will exist on which version it was actually trained. Do note that we recommend using a model trained on a specific major version instead, as other breaking changes might be present as well! ## Data sources ### GnomAD The gnomAD files can be generated through the following scripts (which also download the gnomAD files): -- GRCH37: https://github.com/molgenis/vip/blob/main/utils/create_gnomad_GRCh37.sh - GRCH38: https://github.com/molgenis/vip/blob/main/utils/create_gnomad_GRCh38.sh ### PhyloP PhyloP resources can be downloaded from: -- GRCh37: http://hgdownload.cse.ucsc.edu/goldenpath/hg19/phyloP100way/ - GRCh38: http://hgdownload.cse.ucsc.edu/goldenpath/hg38/phyloP100way/ ### SpliceAI