Question on: merge.group

[apoosakkannu]

Hi, I am working on mothur for processing my data. I need to make a following command for several samples.
make.group(fasta=sample1.fasta-sample2.fasta-sample3.fasta, groups=A-B-C)

I find it difficult to type 351 sample names in the above command. Could you give me any pointer on it to proceed for multiple samples?

I am working on high performance computing remote server.

Thanks in advance!

[pschloss]

You could do this with bash commands like cat and sed from the command line. For example, with the example above...

cat sample1.fasta sample2.fasta sample3.fasta > samples.fasta

> sample.groups
for fasta in sample?.fasta
do
sample_id=`echo $fasta | sed s/.fasta//`
grep ">" $fasta | sed "s/>//" | sed "s/$/\t$sample_id/" >> sample.groups
done

This will use the file name (except for the .fasta) as the group name in the groups file.

[mothur-westcott]

@pschloss, perhaps this would be a good place for some enhancements?

1. Modify make.file so it could look for fasta files in a folder, and generate a file that could be used with the make.group command.
2. Add the file option to make.group

mothur > make.file(inputdir=./, type=fasta)
mothur > make.group(file=current)

[pschloss]

I like that - good idea! Pat

…

On Mon, Aug 8, 2022 at 9:17 AM Sarah Westcott ***@***.***> wrote: @pschloss <https://github.com/pschloss>, perhaps this would be a good place for some enhancements? 1. Modify make.file so it could look for fasta files in a folder, and generate a file that could be used with the make.group command. 2. Add the file option to make.group mothur > make.file(inputdir=./, type=fasta) mothur > make.group(file=current) — Reply to this email directly, view it on GitHub <#90 (comment)>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/AAJUUBGXM2NY7VTGN6NRACLVYECHFANCNFSM55SVSIHA> . You are receiving this because you were mentioned.Message ID: ***@***.***>