From 70c1a9a0880c9cb1f86198f3e9187f1372172eaa Mon Sep 17 00:00:00 2001 From: Will Bradshaw Date: Fri, 12 Apr 2024 15:58:51 -0400 Subject: [PATCH] Added Rosario analysis --- data/2024-04-12_rosario/adapters.fasta | 41 + .../blast/putative-viral.blast.gz | Bin 0 -> 1724317 bytes data/2024-04-12_rosario/bracken_counts.tsv | 105 + .../2024-04-12_rosario/hv_clade_counts.tsv.gz | Bin 0 -> 6122 bytes .../hv_hits_putative_filtered.tsv.gz | Bin 0 -> 181530 bytes .../kraken/SRR5853122.report.gz | Bin 0 -> 75579 bytes .../kraken/SRR5853123.report.gz | Bin 0 -> 71174 bytes .../kraken/SRR5853124.report.gz | Bin 0 -> 83026 bytes .../kraken/SRR5853125.report.gz | Bin 0 -> 80996 bytes .../kraken/SRR5853127.report.gz | Bin 0 -> 68941 bytes .../kraken/SRR5853128.report.gz | Bin 0 -> 83277 bytes .../kraken/SRR5853129.report.gz | Bin 0 -> 77275 bytes .../kraken/SRR5853130.report.gz | Bin 0 -> 87661 bytes .../kraken/SRR5853131.report.gz | Bin 0 -> 70115 bytes .../kraken/SRR5853132.report.gz | Bin 0 -> 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+240 1.298097449424205 SRR5853149 polya 2 ribo_secondary diff --git a/data/2024-04-12_rosario/qc_basic_stats.tsv b/data/2024-04-12_rosario/qc_basic_stats.tsv new file mode 100644 index 0000000..6b66173 --- /dev/null +++ b/data/2024-04-12_rosario/qc_basic_stats.tsv @@ -0,0 +1,131 @@ +sample percent_gc mean_seq_len n_read_pairs percent_duplicates n_bases_approx per_base_sequence_quality per_sequence_quality_scores per_base_sequence_content per_sequence_gc_content per_base_n_content sequence_length_distribution sequence_duplication_levels overrepresented_sequences adapter_content stage +SRR5853122 45 251 587623 6.487984624144509 294800000 fail/fail pass/pass fail/fail warn/warn pass/pass pass/pass pass/pass warn/warn fail/fail raw_concat +SRR5853123 45.5 251 486838 13.830693851954464 244200000 pass/fail pass/pass warn/fail warn/warn pass/pass pass/pass pass/pass warn/warn fail/fail raw_concat +SRR5853124 44 251 789167 6.19355990885731 396000000 fail/fail pass/pass warn/fail warn/warn 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0000000..a3b5498 --- /dev/null +++ b/data/2024-04-12_rosario/sample-metadata.csv @@ -0,0 +1,27 @@ +sample,library,room,na_type +SRR5853122,SRR5853122,Female room 3,DNA +SRR5853123,SRR5853123,Female room 4,DNA +SRR5853125,SRR5853125,Male room 3,DNA +SRR5853128,SRR5853128,Male room 5,DNA +SRR5853132,SRR5853132,Male room 6,RNA +SRR5853133,SRR5853133,Female room 6,RNA +SRR5853135,SRR5853135,NegativeControl,RNA +SRR5853137,SRR5853137,Pool,DNA +SRR5853139,SRR5853139,Male room 6,DNA +SRR5853124,SRR5853124,Male room 4,DNA +SRR5853127,SRR5853127,Male room 2,DNA +SRR5853129,SRR5853129,Female room 1,DNA +SRR5853130,SRR5853130,Male room 1,DNA +SRR5853131,SRR5853131,Negative Control,DNA +SRR5853134,SRR5853134,Male room 1,RNA +SRR5853136,SRR5853136,Female room 6,DNA +SRR5853138,SRR5853138,Female room 5,DNA +SRR5853140,SRR5853140,Pool,RNA +SRR5853141,SRR5853141,Male room 2,RNA +SRR5853143,SRR5853143,Female room 4,RNA +SRR5853144,SRR5853144,Female room 3,RNA +SRR5853145,SRR5853145,Male room 5,RNA +SRR5853146,SRR5853146,Male room 4,RNA +SRR5853147,SRR5853147,Female room 2,RNA +SRR5853148,SRR5853148,Male room 3,RNA +SRR5853149,SRR5853149,Female room 1,RNA diff --git a/data/2024-04-12_rosario/taxid-names.tsv.gz b/data/2024-04-12_rosario/taxid-names.tsv.gz new file mode 120000 index 0000000..7977acc --- /dev/null +++ b/data/2024-04-12_rosario/taxid-names.tsv.gz @@ -0,0 +1 @@ +../2024-04-11_prussin/taxid-names.tsv.gz \ No newline at end of file diff --git a/data/2024-04-12_rosario/taxonomic_composition.tsv b/data/2024-04-12_rosario/taxonomic_composition.tsv new file mode 100644 index 0000000..f43e4d3 --- /dev/null +++ b/data/2024-04-12_rosario/taxonomic_composition.tsv @@ -0,0 +1,209 @@ +sample classification n_reads p_reads +SRR5853122 Filtered 38566 0.0656305148028583 +SRR5853122 Duplicate 30128 0.05127096795053972 +SRR5853122 Ribosomal 486 8.27060887677984e-4 +SRR5853122 Unassigned 474940 0.8082392962835016 +SRR5853122 Bacterial 18602 0.03165635109585568 +SRR5853122 Archaeal 46 7.828148319585857e-5 +SRR5853122 Viral 160 2.7228341981168195e-4 +SRR5853122 Human 24695 0.04202524407655929 +SRR5853123 Filtered 32320 0.06638758683586737 +SRR5853123 Duplicate 56417 0.11588454475616118 +SRR5853123 Ribosomal 437 8.976291908191226e-4 +SRR5853123 Unassigned 371209 0.76248978099491 +SRR5853123 Bacterial 15823 0.03250157136460178 +SRR5853123 Archaeal 54 1.1091985424309523e-4 +SRR5853123 Viral 55 1.1297392561796737e-4 +SRR5853123 Human 10523 0.021614993077779466 +SRR5853124 Filtered 47675 0.060411801304413384 +SRR5853124 Duplicate 38525 0.048817297226062414 +SRR5853124 Ribosomal 931 0.0011797249504857654 +SRR5853124 Unassigned 512043 0.6488398526547613 +SRR5853124 Bacterial 44421 0.05628846619283371 +SRR5853124 Archaeal 62 7.856385277133991e-5 +SRR5853124 Viral 313 3.966207406036999e-4 +SRR5853124 Human 145197 0.1839876730780684 +SRR5853125 Filtered 43849 0.06438138139019971 +SRR5853125 Duplicate 45822 0.0672782425611013 +SRR5853125 Ribosomal 603 8.853559483292761e-4 +SRR5853125 Unassigned 536897 0.788300087214168 +SRR5853125 Bacterial 20646 0.030313530529363572 +SRR5853125 Archaeal 73 1.0718239507137173e-4 +SRR5853125 Viral 100 1.4682519872790648e-4 +SRR5853125 Human 33092 0.04858739476303881 +SRR5853127 Filtered 54862 0.11519410738319857 +SRR5853127 Duplicate 32649 0.06855332310076283 +SRR5853127 Ribosomal 390 8.188856016814451e-4 +SRR5853127 Unassigned 367360 0.7713482426505017 +SRR5853127 Bacterial 13598 0.02855181131195972 +SRR5853127 Archaeal 30 6.299120012934193e-5 +SRR5853127 Viral 43 9.028738685205676e-5 +SRR5853127 Human 7325 0.015380351364914321 +SRR5853128 Filtered 53909 0.07473120664511514 +SRR5853128 Duplicate 35819 0.04965399266952419 +SRR5853128 Ribosomal 639 8.858120359537104e-4 +SRR5853128 Unassigned 597874 0.8288012287696224 +SRR5853128 Bacterial 23151 0.03209301165002246 +SRR5853128 Archaeal 59 8.178859173907499e-5 +SRR5853128 Viral 93 1.2892100053786396e-4 +SRR5853128 Human 9828 0.013624038637485236 +SRR5853129 Filtered 59138 0.09458432828301407 +SRR5853129 Duplicate 40886 0.06539238469646104 +SRR5853129 Ribosomal 518 8.28480537904584e-4 +SRR5853129 Unassigned 486972 0.778854873560755 +SRR5853129 Bacterial 19451 0.03110960413664491 +SRR5853129 Archaeal 39 6.237594783451502e-5 +SRR5853129 Viral 117 1.8712784350354505e-4 +SRR5853129 Human 18120 0.02898082499388236 +SRR5853130 Filtered 51481 0.0708081573594077 +SRR5853130 Duplicate 36257 0.04986871586371758 +SRR5853130 Ribosomal 1401 0.0019269677834643883 +SRR5853130 Unassigned 585737 0.805636208838744 +SRR5853130 Bacterial 24346 0.03348605114648394 +SRR5853130 Archaeal 52 7.152200195585167e-5 +SRR5853130 Viral 267 3.672379715810076e-4 +SRR5853130 Human 27508 0.03783513903464553 +SRR5853131 Filtered 27032 0.06102568849176338 +SRR5853131 Duplicate 8999 0.020315558254564173 +SRR5853131 Ribosomal 625 0.0014109594298369382 +SRR5853131 Unassigned 326306 0.7366472443397951 +SRR5853131 Bacterial 52698 0.11896758405367515 +SRR5853131 Archaeal 32 7.224112280765124e-5 +SRR5853131 Viral 37 8.352879824634674e-5 +SRR5853131 Human 27232 0.0614771955093112 +SRR5853132 Filtered 180032 0.2860106853663225 +SRR5853132 Duplicate 188298 0.2991425970555668 +SRR5853132 Ribosomal 6163 0.009790947464409915 +SRR5853132 Unassigned 55010 0.08739250689878134 +SRR5853132 Bacterial 27309 0.043384874948169776 +SRR5853132 Archaeal 9 1.4297992403000037e-5 +SRR5853132 Viral 706 0.001121598070724225 +SRR5853132 Human 171932 0.27314249220362247 +SRR5853133 Filtered 140657 0.20170938945255082 +SRR5853133 Duplicate 171784 0.24634711217868283 +SRR5853133 Ribosomal 8505 0.012196608468074428 +SRR5853133 Unassigned 309288 0.44353493708098807 +SRR5853133 Bacterial 26364 0.0378073351736995 +SRR5853133 Archaeal 16 2.294482486645395e-5 +SRR5853133 Viral 1018 0.0014598644821281325 +SRR5853133 Human 39693 0.05692180833900979 +SRR5853134 Filtered 213037 0.3716107312308122 +SRR5853134 Duplicate 282874 0.49343078425900083 +SRR5853134 Ribosomal 2197 0.0038323332403014236 +SRR5853134 Unassigned 24519 0.04276967624895339 +SRR5853134 Bacterial 8722 0.015214205972648618 +SRR5853134 Archaeal 2 3.488696622941669e-6 +SRR5853134 Viral 411 7.16927156014513e-4 +SRR5853134 Human 41518 0.0724218531956461 +SRR5853135 Filtered 229989 0.3639463802218913 +SRR5853135 Duplicate 323512 0.5119419683478101 +SRR5853135 Ribosomal 2612 0.004133362661429808 +SRR5853135 Unassigned 14575 0.023064226948828273 +SRR5853135 Bacterial 11279 0.017848467633333386 +SRR5853135 Archaeal 7 1.1077158740432104e-5 +SRR5853135 Viral 40 6.32980499453263e-5 +SRR5853135 Human 49917 0.07899121897802133 +SRR5853136 Filtered 63023 0.07485094657830352 +SRR5853136 Duplicate 38526 0.045756431269151285 +SRR5853136 Ribosomal 1062 0.0012613126202522624 +SRR5853136 Unassigned 672012 0.7981329722796265 +SRR5853136 Bacterial 42760 0.05078505427682368 +SRR5853136 Archaeal 90 1.0689090002137819e-4 +SRR5853136 Viral 1210 0.0014370887669540844 +SRR5853136 Human 23297 0.027669303308867195 +SRR5853137 Filtered 50348 0.07353830424304389 +SRR5853137 Duplicate 26704 0.039003870590812825 +SRR5853137 Ribosomal 830 0.0012122982545826335 +SRR5853137 Unassigned 536555 0.7836923975754035 +SRR5853137 Bacterial 32773 0.04786825385233331 +SRR5853137 Archaeal 60 8.763601840356387e-5 +SRR5853137 Viral 465 6.791791426276199e-4 +SRR5853137 Human 36915 0.053918060322792664 +SRR5853138 Filtered 49074 0.07166608982138241 +SRR5853138 Duplicate 22909 0.0334555661188827 +SRR5853138 Ribosomal 691 0.001009114155491202 +SRR5853138 Unassigned 581361 0.8490008893639952 +SRR5853138 Bacterial 25732 0.03757818444153344 +SRR5853138 Archaeal 72 1.0514648219300513e-4 +SRR5853138 Viral 237 3.461071705519752e-4 +SRR5853138 Human 4683 0.006838902445970042 +SRR5853139 Filtered 71179 0.09694611365654607 +SRR5853139 Duplicate 71795 0.09778510838831292 +SRR5853139 Ribosomal 726 9.88815219582355e-4 +SRR5853139 Unassigned 554369 0.7550530364526867 +SRR5853139 Bacterial 27634 0.037637630548125064 +SRR5853139 Archaeal 64 8.716828381993212e-5 +SRR5853139 Viral 459 6.251600355210757e-4 +SRR5853139 Human 7986 0.010876967415405905 +SRR5853140 Filtered 204090 0.31619604185581 +SRR5853140 Duplicate 199424 0.3089670216622718 +SRR5853140 Ribosomal 5362 0.008307330963941659 +SRR5853140 Unassigned 117246 0.18164888590046696 +SRR5853140 Bacterial 48788 0.07558710612994884 +SRR5853140 Archaeal 9 1.3943673755217259e-5 +SRR5853140 Viral 2142 0.0033185943537417076 +SRR5853140 Human 68393 0.10596107546006377 +SRR5853141 Filtered 123160 0.20426678077477414 +SRR5853141 Duplicate 151417 0.2511323736974178 +SRR5853141 Ribosomal 11146 0.018486176831078536 +SRR5853141 Unassigned 200657 0.3327992808535552 +SRR5853141 Bacterial 47177 0.07824532247979474 +SRR5853141 Archaeal 9 1.492693266460675e-5 +SRR5853141 Viral 317 5.257597394089267e-4 +SRR5853141 Human 69054 0.11452937869130606 +SRR5853143 Filtered 106621 0.17727089838359453 +SRR5853143 Duplicate 158098 0.2628579219164098 +SRR5853143 Ribosomal 17765 0.02953655949376349 +SRR5853143 Unassigned 70970 0.11799660159146608 +SRR5853143 Bacterial 42329 0.07037731645434926 +SRR5853143 Archaeal 17 2.8264650233266494e-5 +SRR5853143 Viral 239 3.9736772975004076e-4 +SRR5853143 Human 205419 0.3415350697804335 +SRR5853144 Filtered 255004 0.3901082342142502 +SRR5853144 Duplicate 111161 0.17005545569281372 +SRR5853144 Ribosomal 9743 0.014904960416108923 +SRR5853144 Unassigned 146268 0.22376257314414655 +SRR5853144 Bacterial 33171 0.050745401002027 +SRR5853144 Archaeal 8 1.223849772440433e-5 +SRR5853144 Viral 221 3.380884996366696e-4 +SRR5853144 Human 98099 0.15007304853329254 +SRR5853145 Filtered 100561 0.14096690899201814 +SRR5853145 Duplicate 149229 0.2091899529834615 +SRR5853145 Ribosomal 185389 0.25987922048429557 +SRR5853145 Unassigned 156596 0.219517050153778 +SRR5853145 Bacterial 25056 0.03512362517978149 +SRR5853145 Archaeal 13 1.8223464532932603e-5 +SRR5853145 Viral 68 9.532273755687824e-5 +SRR5853145 Human 96454 0.1352096960045755 +SRR5853146 Filtered 260400 0.35004563767050273 +SRR5853146 Duplicate 202822 0.27264576161139287 +SRR5853146 Ribosomal 25024 0.033638794305171506 +SRR5853146 Unassigned 155426 0.20893315391926098 +SRR5853146 Bacterial 36379 0.04890288115520437 +SRR5853146 Archaeal 113 1.5190152479557147e-4 +SRR5853146 Viral 2833 0.0038082922101403006 +SRR5853146 Human 60906 0.08187357760353164 +SRR5853147 Filtered 203157 0.2736906344850442 +SRR5853147 Duplicate 222062 0.2991592200860314 +SRR5853147 Ribosomal 55676 0.07500602866546228 +SRR5853147 Unassigned 85321 0.11494341137592333 +SRR5853147 Bacterial 51692 0.0696388324192664 +SRR5853147 Archaeal 17 2.2902192817602896e-5 +SRR5853147 Viral 339 4.5669666853925773e-4 +SRR5853147 Human 124023 0.1670822741069155 +SRR5853148 Filtered 342549 0.5256326253780561 +SRR5853148 Duplicate 153817 0.23602822818859917 +SRR5853148 Ribosomal 8515 0.013066048375835713 +SRR5853148 Unassigned 75681 0.11613054693266267 +SRR5853148 Bacterial 28099 0.0431171924031248 +SRR5853148 Archaeal 5 7.672371330496601e-6 +SRR5853148 Viral 233 3.5753250400114163e-4 +SRR5853148 Human 42790 0.06566015384638992 +SRR5853149 Filtered 38636 0.052449455834859424 +SRR5853149 Duplicate 12549 0.017035620179926776 +SRR5853149 Ribosomal 1524 0.0020688728308397805 +SRR5853149 Unassigned 65794 0.08931720408941766 +SRR5853149 Bacterial 2813 0.00381872655718655 +SRR5853149 Archaeal 2 2.7150562084511556e-6 +SRR5853149 Viral 12 1.6290337250706933e-5 +SRR5853149 Human 615303 0.8352911151143106 diff --git a/data/2024-04-12_rosario/viral-taxids.tsv.gz b/data/2024-04-12_rosario/viral-taxids.tsv.gz new file mode 120000 index 0000000..954db4a --- /dev/null +++ b/data/2024-04-12_rosario/viral-taxids.tsv.gz @@ -0,0 +1 @@ +../2024-04-11_prussin/viral-taxids.tsv.gz \ No newline at end of file diff --git a/docs/index.html b/docs/index.html index 4090106..84cd8a9 100644 --- a/docs/index.html +++ b/docs/index.html @@ -164,10 +164,10 @@

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