From 07772db6532bae9c2f17aa95a1aa8cf5789afc3c Mon Sep 17 00:00:00 2001 From: maxibor Date: Mon, 29 Apr 2019 15:53:49 +0200 Subject: [PATCH 1/6] change logo location --- README.md | 20 ++++++++++---------- 1 file changed, 10 insertions(+), 10 deletions(-) diff --git a/README.md b/README.md index 001e26d..4f2095e 100644 --- a/README.md +++ b/README.md @@ -3,24 +3,24 @@ [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](http://bioconda.github.io/) [![Docker](https://img.shields.io/docker/automated/nfcore/coproid.svg)](https://hub.docker.com/r/nfcore/coproid) -# ![logo_coproid](docs/source/_static/img/coproid_logo_small.jpg) ![logo_nf_core](assets/img/coproID_nf-core_logo_small.png) +# ![logo_coproid](assets/img/coproid_logo_small.jpg) ![logo_nf_core](assets/img/coproID_nf-core_logo_small.png) ## Documentation The nf-core/coproid pipeline comes with documentation about the pipeline, found in the `docs/` directory: -1. [Installation](https://nf-co.re/usage/installation) -2. Pipeline configuration - * [Local installation](https://nf-co.re/usage/local_installation) - * [Adding your own system config](https://nf-co.re/usage/adding_own_config) - * [Reference genomes](https://nf-co.re/usage/reference_genomes) -3. [Running the pipeline](docs/usage.md) -4. [Output and how to interpret the results](docs/output.md) -5. [Troubleshooting](https://nf-co.re/usage/troubleshooting) +1. [Installation](https://nf-co.re/usage/installation) +2. Pipeline configuration + - [Local installation](https://nf-co.re/usage/local_installation) + - [Adding your own system config](https://nf-co.re/usage/adding_own_config) + - [Reference genomes](https://nf-co.re/usage/reference_genomes) +3. [Running the pipeline](docs/usage.md) +4. [Output and how to interpret the results](docs/output.md) +5. [Troubleshooting](https://nf-co.re/usage/troubleshooting) ## Introduction -**CoproID** helps you to identify the *"true maker"* of a sequenced Coprolite by checking the microbiome composition and the endogenous DNA. +**CoproID** helps you to identify the _"true maker"_ of a sequenced Coprolite by checking the microbiome composition and the endogenous DNA. The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. From 3234dee58354524fc1892bc06a1f1f916decb970 Mon Sep 17 00:00:00 2001 From: maxibor Date: Mon, 29 Apr 2019 15:54:55 +0200 Subject: [PATCH 2/6] add logo in multiqc report --- assets/multiqc_config.yaml | 8 ++++++-- main.nf | 3 ++- 2 files changed, 8 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml index 8537378..8a27b17 100644 --- a/assets/multiqc_config.yaml +++ b/assets/multiqc_config.yaml @@ -1,5 +1,5 @@ -title: "Metrics Results of coproID" -intro_text: "This reports summarises analysis results generated by coproID" +title: "Results of coproID" +intro_text: "This reports summarizes results generated by coproID" report_comment: > This report has been generated by the nf-core/coproid @@ -8,6 +8,10 @@ report_comment: > export_plots: true +custom_logo: 'coproid_logo_small.jpg' +custom_logo_url: 'https://github.com/nf-core/coproid' +custom_logo_title: 'github.com/nf-core/coproid' + report_section_order: nf-core/coproid-software-versions: order: -1000 diff --git a/main.nf b/main.nf index 08c2050..3e4698f 100644 --- a/main.nf +++ b/main.nf @@ -80,6 +80,7 @@ DEFAULT VARIABLE VALUES SETUP bowtie_setting = '' collapse_setting = '' report_template = "$baseDir/templates/coproID_report.ipynb" +coproid_logo = file("$baseDir/assets/img/coproid_logo_small.jpg") // Show help message @@ -1210,7 +1211,6 @@ process get_software_versions { file "software_versions.csv" script: - // TODO nf-core: Get all tools to print their version number here """ echo $workflow.manifest.version > v_pipeline.txt echo $workflow.nextflow.version > v_nextflow.txt @@ -1243,6 +1243,7 @@ process multiqc { file ('DamageProfiler/*') from dmgProf2_ch.collect() file ('software_versions/*') from software_versions_yaml.collect() file(multiqc_conf) from ch_multiqc_config + file logo from coproid_logo output: file 'multiqc_report.html' into multiqc_report From 887e45392b8dfdbce22f690bea906b96c528da40 Mon Sep 17 00:00:00 2001 From: maxibor Date: Mon, 29 Apr 2019 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z^oi-o<6n%*e`^n?@vkDkeqtQ_b<%THR!bzc4o??xG{jF4^v-}8t~a|l41zfXWU+oXP$<{s*hNsXV9Mhk%PdviInb@A;G{^?7!0Vr>=%h9#z zuFlB(fq0aYmS$)3E#ae;@?NKeK3q5zihrgp+4`VkECvE1KoAYiJ%MgtSOAYrw8Ci@ z2Ek~FOmORd3Y-ioZ#Zc<>v-=~Qv(l|ieVtmeuqWF~#e6f_ozlY*4sO%gGL}J~v=)e)!5C0^9 z0`BVKc8vV&wtJm+zHz+H>ZoCf*_y#1%bUug%XhOk z%VZbU-t9sAMT;QR@eXZtfN2@JuT^5XdijK43io|KxP)Vdxp#)Cj;~zi`v~zwZ+CMY NjnApII{ literal 0 HcmV?d00001 From 6284da897d4988c93e46672939f0b36be354d069 Mon Sep 17 00:00:00 2001 From: maxibor Date: Mon, 29 Apr 2019 16:03:00 +0200 Subject: [PATCH 4/6] add python version scraping --- bin/scrape_software_versions.py | 4 ++-- main.nf | 1 + 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index d8e3dff..8fd44f7 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -3,7 +3,6 @@ from collections import OrderedDict import re -# TODO nf-core: Add additional regexes for new tools in process get_software_versions regexes = { 'nf-core/coproid': ['v_pipeline.txt', r"(\S+)"], 'Nextflow': ['v_nextflow.txt', r"(\S+)"], @@ -14,7 +13,8 @@ 'Kraken2': ['v_kraken2.txt', r"Kraken\sversion\s(\S+)"], 'MultiQC': ['v_multiqc.txt', r"multiqc, version (\S+)"], 'Sourcepredict': ['v_sourcepredict.txt', r"SourcePredict\sv(\d*\.\d*)"], - 'Samtools': ['v_samtools.txt', r"samtools\s(\S+)"] + 'Samtools': ['v_samtools.txt', r"samtools\s(\S+)"], + 'Python': ['v_python.txt', r'Python\s(\S+)'] } results = OrderedDict() results['nf-core/coproid'] = 'N/A' diff --git a/main.nf b/main.nf index 3e4698f..77dd9af 100644 --- a/main.nf +++ b/main.nf @@ -1221,6 +1221,7 @@ process get_software_versions { bedtools --version > v_bedtools.txt kraken2 --version > v_kraken2.txt bowtie2 --version > v_bowtie2.txt + python --version > v_python.txt AdapterRemoval --version 2> v_adapterremoval.txt scrape_software_versions.py &> software_versions_mqc.yaml """ From d4ecabc5cb15a17f058f8af78ec0f280159539ac Mon Sep 17 00:00:00 2001 From: maxibor Date: Mon, 29 Apr 2019 16:03:42 +0200 Subject: [PATCH 5/6] make doc both nf-core and sphinx compatible --- .gitignore | 3 + docs/Makefile | 6 +- docs/{source => }/conf.py | 32 +-- docs/configuration.md | 10 + docs/{source => }/index.rst | 12 +- docs/installation.md | 6 + docs/introduction.md | 27 +++ docs/source/_static/img/coproid_dag.png | Bin 351727 -> 0 bytes .../source/_static/img/coproid_logo_small.jpg | Bin 22416 -> 0 bytes docs/source/installation.md | 10 - docs/source/intro.md | 88 --------- docs/source/methods.md | 35 ---- docs/source/output.md | 51 ----- docs/source/troubleshooting.md | 13 -- docs/source/usage.md | 1 - docs/usage.md | 185 ++++++------------ 16 files changed, 134 insertions(+), 345 deletions(-) rename docs/{source => }/conf.py (88%) create mode 100644 docs/configuration.md rename docs/{source => }/index.rst (65%) create mode 100644 docs/installation.md create mode 100644 docs/introduction.md delete mode 100644 docs/source/_static/img/coproid_dag.png delete mode 100644 docs/source/_static/img/coproid_logo_small.jpg delete mode 100644 docs/source/installation.md delete mode 100644 docs/source/intro.md delete mode 100644 docs/source/methods.md delete mode 100644 docs/source/output.md delete mode 100644 docs/source/troubleshooting.md delete mode 120000 docs/source/usage.md diff --git a/.gitignore b/.gitignore index b3877ba..6447efb 100644 --- a/.gitignore +++ b/.gitignore @@ -7,3 +7,6 @@ tests/test_data *.ipynb_checkpoints docs/build/ test/ +_build +_static +_templates diff --git a/docs/Makefile b/docs/Makefile index 575a4bc..1567842 100644 --- a/docs/Makefile +++ b/docs/Makefile @@ -4,9 +4,9 @@ # You can set these variables from the command line. SPHINXOPTS = SPHINXBUILD = sphinx-build -SPHINXPROJ = coproid -SOURCEDIR = source -BUILDDIR = build +SPHINXPROJ = coproID +SOURCEDIR = . +BUILDDIR = _build # Put it first so that "make" without argument is like "make help". help: diff --git a/docs/source/conf.py b/docs/conf.py similarity index 88% rename from docs/source/conf.py rename to docs/conf.py index 2937128..5bdb5ea 100644 --- a/docs/source/conf.py +++ b/docs/conf.py @@ -22,16 +22,17 @@ '.md': CommonMarkParser, } + # -- Project information ----------------------------------------------------- -project = 'coproid' +project = 'nf-core/coproID' copyright = '2019, Maxime Borry' author = 'Maxime Borry' # The short X.Y version version = '' # The full version, including alpha/beta/rc tags -release = '' +release = '1.0' # -- General configuration --------------------------------------------------- @@ -48,9 +49,7 @@ 'sphinx.ext.doctest', 'sphinx.ext.intersphinx', 'sphinx.ext.todo', - 'sphinx.ext.coverage', 'sphinx.ext.mathjax', - 'sphinx.ext.ifconfig', 'sphinx.ext.viewcode', 'sphinx.ext.githubpages', ] @@ -61,8 +60,8 @@ # The suffix(es) of source filenames. # You can specify multiple suffix as a list of string: # -# source_suffix = ['.rst', '.md'] source_suffix = ['.rst', '.md'] +# source_suffix = '.rst' # The master toctree document. master_doc = 'index' @@ -77,7 +76,7 @@ # List of patterns, relative to source directory, that match files and # directories to ignore when looking for source files. # This pattern also affects html_static_path and html_extra_path . -exclude_patterns = [] +exclude_patterns = ['_build', 'Thumbs.db', '.DS_Store'] # The name of the Pygments (syntax highlighting) style to use. pygments_style = 'sphinx' @@ -88,6 +87,7 @@ # The theme to use for HTML and HTML Help pages. See the documentation for # a list of builtin themes. # +# html_theme = 'alabaster' html_theme = 'sphinx_rtd_theme' # Theme options are theme-specific and customize the look and feel of a theme @@ -115,7 +115,7 @@ # -- Options for HTMLHelp output --------------------------------------------- # Output file base name for HTML help builder. -htmlhelp_basename = 'coproiddoc' +htmlhelp_basename = 'coproIDdoc' # -- Options for LaTeX output ------------------------------------------------ @@ -142,7 +142,7 @@ # (source start file, target name, title, # author, documentclass [howto, manual, or own class]). latex_documents = [ - (master_doc, 'coproid.tex', 'coproid Documentation', + (master_doc, 'coproID.tex', 'coproID Documentation', 'Maxime Borry', 'manual'), ] @@ -152,7 +152,7 @@ # One entry per manual page. List of tuples # (source start file, name, description, authors, manual section). man_pages = [ - (master_doc, 'coproid', 'coproid Documentation', + (master_doc, 'coproid', 'coproID Documentation', [author], 1) ] @@ -163,8 +163,8 @@ # (source start file, target name, title, author, # dir menu entry, description, category) texinfo_documents = [ - (master_doc, 'coproid', 'coproid Documentation', - author, 'coproid', 'One line description of project.', + (master_doc, 'coproID', 'coproID Documentation', + author, 'coproID', 'One line description of project.', 'Miscellaneous'), ] @@ -201,3 +201,13 @@ # If true, `todo` and `todoList` produce output, else they produce nothing. todo_include_todos = True + +# doc_root = "file:///Users/borry/Documents/GitHub/coproID/docs/_build/html/" + + +# def setup(app): +# app.add_config_value('recommonmark_config', { +# 'url_resolver': lambda url: doc_root + url, +# 'auto_toc_tree_section': 'Contents', +# }, True) +# app.add_transform(AutoStructify) diff --git a/docs/configuration.md b/docs/configuration.md new file mode 100644 index 0000000..ebf5787 --- /dev/null +++ b/docs/configuration.md @@ -0,0 +1,10 @@ +# Configuration + +This part of the documentation (common to all nf-core pipelines) is hosted on [nf-co.re +](https://nf-co.re/) + +## Pipeline configuration + +- [Local installation](https://nf-co.re/usage/local_installation) +- [Adding your own system config](https://nf-co.re/usage/adding_own_config) +- [Reference genomes](https://nf-co.re/usage/reference_genomes) diff --git a/docs/source/index.rst b/docs/index.rst similarity index 65% rename from docs/source/index.rst rename to docs/index.rst index c0cc7d1..7b67a5b 100644 --- a/docs/source/index.rst +++ b/docs/index.rst @@ -1,21 +1,21 @@ -.. coproid documentation master file, created by - sphinx-quickstart on Tue Apr 23 18:30:28 2019. +.. coproID documentation master file, created by + sphinx-quickstart on Mon Apr 29 14:50:51 2019. You can adapt this file completely to your liking, but it should at least contain the root `toctree` directive. -Welcome to coproid's documentation! +Welcome to coproID's documentation! =================================== .. toctree:: :maxdepth: 2 :caption: Contents: - intro + introduction installation + configuration usage - methods output - troubleshooting + Indices and tables diff --git a/docs/installation.md b/docs/installation.md new file mode 100644 index 0000000..4da1c3d --- /dev/null +++ b/docs/installation.md @@ -0,0 +1,6 @@ +# Installation + +This part of the documentation (common to all nf-core pipelines) is hosted on [nf-co.re +](https://nf-co.re/) + +[nf-co.re/usage/installation](https://nf-co.re/usage/installation) diff --git a/docs/introduction.md b/docs/introduction.md new file mode 100644 index 0000000..be2e958 --- /dev/null +++ b/docs/introduction.md @@ -0,0 +1,27 @@ +# Introduction + +![logo](../assets/img/coproid_logo_small.jpg) ![nf-core-logo](../assets/img/coproID_nf-core_logo_small.png) + +**coproID** (**CO**prolite **ID**entification) is a tool developed at the +[Max Planck insitute for the Science of Human History](http://www.shh.mpg.de/en) +by [Maxime Borry](https://github.com/maxibor) + +The purpose of **coproID** is to help identify the host of given sequence microbiome when there is a doubt between species. + +**coproID** is a pipeline developed using [Nextflow](https://www.nextflow.io/) and made available through [nf-core](https://github.com/nf-core) + +Even though it was developed with coprolite host identification in mind, it can be applied to any microbiome, provided they contain host DNA. + +## Quick start + +Example: + + nextflow run maxibor/coproid --genome1 'GRCh37' --genome2 'CanFam3.1' --name1 'Homo_sapiens' --name2 'Canis_familiaris' --reads '*_R{1,2}.fastq.gz' + +## coproID example workFlow + +![dag](../assets/img/coproid_dag.png) + +## How to cite coproID + +The coproID article is coming. 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[Installation](https://nf-co.re/usage/installation) -2. Pipeline configuration - * [Local installation](https://nf-co.re/usage/local_installation) - * [Adding your own system config](https://nf-co.re/usage/adding_own_config) - * [Reference genomes](https://nf-co.re/usage/reference_genomes) diff --git a/docs/source/intro.md b/docs/source/intro.md deleted file mode 100644 index 741f258..0000000 --- a/docs/source/intro.md +++ /dev/null @@ -1,88 +0,0 @@ -Introduction -============ - - - -**coproID** (**CO**prolite **ID**entification) is a tool developed at the [Max Planck insitute for the Science of Human History](http://www.shh.mpg.de/en) by [Maxime Borry](https://github.com/maxibor) - -The purpose of **coproID** is to help identify the host of given sequenced microbiome when there is a doubt between species. - -**coproID** is a pipeline developed using [Nextflow](https://www.nextflow.io/) and made available through [nf-core](https://github.com/nf-core) - -Even though it was developed with coprolite host identification in mind, it can be applied to any microbiome, provided they contain host DNA. - - -## Quick start - -Example: -``` -nextflow run maxibor/coproid --genome1 'GRCh37' --genome2 'CanFam3.1' --name1 'Homo_sapiens' --name2 'Canis_familiaris' --reads '*_R{1,2}.fastq.gz' -``` - -## coproID help menu - -``` -$ nextflow run nf-core/coproid --help -N E X T F L O W ~ version 19.01.0 -Launching `./main.nf` [nasty_ride] - revision: 362ad5aeaf ----------------------------------------------------- - ,--./,-. - ___ __ __ __ ___ /,-._.--~' - |\ | |__ __ / ` / \ |__) |__ } { - | \| | \__, \__/ | \ |___ \`-._,-`-, - `._,._,' - nf-core/coproid v1.0dev ----------------------------------------------------- - - - coproID: Coprolite Identification - Homepage: https://github.com/nf-core/coproid - Author: Maxime Borry - Version 1.0dev -========================================= -Usage: -The typical command for running the pipeline is as follows: -nextflow run maxibor/coproid --genome1 'GRCh37' --genome2 'CanFam3.1' --name1 'Homo_sapiens' --name2 'Canis_familiaris' --reads '*_R{1,2}.fastq.gz' -Mandatory arguments: - --reads Path to input data (must be surrounded with quotes) - --name1 Name of candidate 1. Example: "Homo_sapiens" - --fasta1 Path to human genome fasta file (must be surrounded with quotes). Must be provided if --genome1 is not provided - --genome1 Name of iGenomes reference for Homo_sapiens. Must be provided if --fasta1 is not provided - --name2 Name of candidate 2. Example: "Canis_familiaris" - --fasta2 Path to canidate organism 2 genome fasta file (must be surrounded with quotes). Must be provided if --genome2 is not provided - --genome2 Name of iGenomes reference for candidate organism 2. Must be provided if --fasta2 is not provided - --krakendb Path to MiniKraken2_v2_8GB Database - -Options: - --name3 Name of candidate 1. Example: "Sus_scrofa" - --fasta2 Path to canidate organism 3 genome fasta file (must be surrounded with quotes). Must be provided if --genome3 is not provided - --genome2 Name of iGenomes reference for candidate organism 3. Must be provided if --fasta3 is not provided - --adna Specified if data is modern (false) or ancient DNA (true). Default = true - --phred Specifies the fastq quality encoding (33 | 64). Defaults to 33 - --singleEnd Specified if reads are single-end (true | false). Default = false - --index1 Path to Bowtie2 index of genome candidate 1, in the form of "/path/to/bowtie_index/basename" - --index2 Path to Bowtie2 index genome candidate 2 Coprolite maker's genome, in the form of "/path/to/bowtie_index/basename" - --index3 Path to Bowtie2 index genome candidate 3 Coprolite maker's genome, in the form of "/path/to/bowtie_index/basename" - --collapse Specifies if AdapterRemoval should merge the paired-end sequences or not (true | false). Default = true - --identity Identity threshold to retain read alignment. Default = 0.95 - --pmdscore Minimum PMDscore to retain read alignment. Default = 3 - --library DNA preparation library type ( classic | UDGhalf). Default = classic - --bowtie Bowtie settings for sensivity (very-fast | very-sensitive). Default = very-sensitive - --minKraken Minimum number of Kraken hits per Taxonomy ID to report. Default = 50 - --removeHuman Remove human reads for metagenomic taxonomic classification. Default = false - - Other options: - --results The output directory where the results will be saved. Defaults to ./results - --email Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - --maxMultiqcEmailFileSize Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - -name Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. - --help --h Shows this help page -``` - -## coproID example workFlow - -![](_static/img/coproid_dag.png) - -## How to cite coproID - -The coproID article is coming. \ No newline at end of file diff --git a/docs/source/methods.md b/docs/source/methods.md deleted file mode 100644 index 74558e5..0000000 --- a/docs/source/methods.md +++ /dev/null @@ -1,35 +0,0 @@ -Methods -======= - -## Pipeline overview: - - **0.1 - Fastqc:** Quality reporting on the `.fastq` files - - - **0.2 - Bowtie2:** Indexing of each genomes (if no index provided) - - - **1 - AdapterRemoval:** Adapter trimming, quality filtering, and read merging (if specified) - - - **2 - Bowtie2:** Reads alignment on each genome - - - **3 - PMDtools:** Filtering out reads without Post Mortem Damage (PMD). - - - **4 - countBp2/3genomes:** Count aligned bp in each read passing an identity threshold, on each genome, and compute ratio - - - **5 - damageProfiler:** DNA damage assessment - - - **6 - generateReport:** Make plots and write html report - - - **8 - MultiQC:** Generates QC report - -## Read ratio computation - -The following equation is used to compute the read ratio. - -$$NormalizedReadRatio_{genome1} = \log2\left(\frac{\frac{N_{\ aDNA\ bp \ aligned \ genome1}}{size_{genome1} }}{\frac{N_{\ aDNA\ bp \ aligned \ genome1}}{size_{genome1} }+\frac{N_{ \ aDNA \ bp \ aligned \ genome2}}{size_{genome2}}}\right)$$ - - -**A read is considered as an aDNA read when `PMDscore > --pmdscore`** - - -Because of the log2, an increase of the NormalizedReadRatio by 1 is equivalent to a two fold increase of the number of base pairs. - -Note that only basepairs originating from Post Mortem Damage carrying reads (as identified by PMDtools) and aligned to a reference genome with an identity greater than the predefined threshold, are taken into account for the calculation of the NormalizedReadRatio. diff --git a/docs/source/output.md b/docs/source/output.md deleted file mode 100644 index da8afa2..0000000 --- a/docs/source/output.md +++ /dev/null @@ -1,51 +0,0 @@ -Output -====== - -This document describes the output produced by the coproID pipeline. - -## `multiqc_report.html` - -### FastQC -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your reads. It provides information about the quality score distribution across your reads, the per base sequence content (%T/A/G/C). You get information about adapter contamination and other overrepresented sequences. - -For further reading and documentation see the [FastQC help](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. - -### AdapterRemoval -[AdapterRemoval](https://github.com/MikkelSchubert/adapterremoval) searches for and removes remnant adapter sequences from High-Throughput Sequencing (HTS) data and (optionally) trims low quality bases from the 3' end of reads following adapter removal. AdapterRemoval can analyze both single end and paired end data, and can be used to merge overlapping paired-ended reads into (longer) consensus sequences. - -### Bowtie2 -[Bowtie 2](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml) is an ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences. -This plot shows the number of reads aligning to the reference in different ways. - -### DamageProfiler -[DamageProfiler](https://github.com/Integrative-Transcriptomics/DamageProfiler) calculates damage profiles of mapped reads. -These plots represents the damage patterns and read length distribution. - -## `coproID_report.html` - -### coproID summary table -This table summarizes the read ratios and microbiome source proportions as computed by coproID and sourcepredict. -You can download the table in `.csv` format by clicking on the green "Download" button. - -### microbiome profile embedding -This interactive plot shows the embedding of the microbiome samples by [sourcepredict](https://github.com/maxibor/sourcepredict) - -### Damage plots -These plots represents the damage patterns computed by DamageProfiler - -## `coproID_result.csv` -This table summarizes the read ratios and microbiome source proportions as computed by coproID and sourcepredict. - -## `kraken` -This directory contains the merged OTU count for all samples of the run, as counted by [Kraken2](https://ccb.jhu.edu/software/kraken2/) - -## `damageprofiler` -This directory contains all of the output files of DamageProfiler (see multiqc section above) - -## `alignments` -This directory contains the alignment `.bam` files for aligned and aligned sequences to each target genome. - -## `pmdtools` -This directory contains the alignment `.bam` files for aligned and aligned **ancient DNA** sequences to each target genome, according to [PMDTools](https://github.com/pontussk/PMDtools). diff --git a/docs/source/troubleshooting.md b/docs/source/troubleshooting.md deleted file mode 100644 index 2ccd5d8..0000000 --- a/docs/source/troubleshooting.md +++ /dev/null @@ -1,13 +0,0 @@ -Troubleshooting -=============== - -## Known issues - -- On Mac OS X, the nextflow log reporting (`-with-report`) may not work with Nextflow v0.31.1. This should be fixed in future Nextflow releases. -- If the DNA damage levels are too low, mapDamage may crash. -- If you run into the following error `CondaVerificationError: The package for openjdk located at...`, running `conda clean --all` might fix it -- If you run into an error stating `Failed to create Conda environment`, please relaunch **coproID** with the flag `-resume`. It will restart just before it crashed without loosing any results. This error is due to a network communication problem that sometimes happens with the anaconda servers. - -## Other issues - -If you find a specific issue, please open an [issue](https://github.com/maxibor/coproid/issues) on [GitHub](https://github.com/maxibor/coproid/issues) diff --git a/docs/source/usage.md b/docs/source/usage.md deleted file mode 120000 index f2c68c1..0000000 --- a/docs/source/usage.md +++ /dev/null @@ -1 +0,0 @@ -../usage.md \ No newline at end of file diff --git a/docs/usage.md b/docs/usage.md index 21cc00d..9b8c3b7 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,75 +1,6 @@ -# nf-core/coproid: Usage - - -* [Table of contents](#Tableofcontents) -* [Introduction](#Introduction) -* [Running the pipeline](#Runningthepipeline) - * [Updating the pipeline](#Updatingthepipeline) - * [Reproducibility](#Reproducibility) -* [Main arguments](#Mainarguments) - * [`-profile`](#-profile) - * [`--reads`](#--reads) - * [`--singleEnd`](#--singleEnd) - * [`--name1`](#--name1) - * [`--name2`](#--name2) - * [`--krakenDB`](#--krakenDB) -* [Reference genomes](#Referencegenomes) - * [`--genome1` (using iGenomes)](#--genome1usingiGenomes) - * [`--fasta1`](#--fasta1) - * [`--fasta2`](#--fasta2) - * [`--genome2`](#--genome2) - * [`--igenomesIgnore`](#--igenomesIgnore) -* [Settings](#Settings) - * [`--adna`](#--adna) - * [`--phred`](#--phred) - * [`--collapse`](#--collapse) - * [`--identity`](#--identity) - * [`--pmdscore`](#--pmdscore) - * [`--library`](#--library) - * [`--bowtie`](#--bowtie) - * [`--minKraken`](#--minKraken) - * [`--endo1`](#--endo1) - * [`--endo2`](#--endo2) - * [`--endo3`](#--endo3) -* [Other coproID parameters](#OthercoproIDparameters) - * [`--name3`](#--name3) - * [`--fasta3`](#--fasta3) - * [`--genome3`](#--genome3) - * [`--index1`](#--index1) - * [`--index2`](#--index2) - * [`--index3`](#--index3) -* [Job resources](#Jobresources) - * [Automatic resubmission](#Automaticresubmission) - * [Custom resource requests](#Customresourcerequests) -* [AWS Batch specific parameters](#AWSBatchspecificparameters) - * [`--awsqueue`](#--awsqueue) - * [`--awsregion`](#--awsregion) -* [Other command line parameters](#Othercommandlineparameters) - * [`--outdir`](#--outdir) - * [`--email`](#--email) - * [`-name`](#-name) - * [`-resume`](#-resume) - * [`-c`](#-c) - * [`--custom_config_version`](#--custom_config_version) - * [`--custom_config_base`](#--custom_config_base) - * [`--max_memory`](#--max_memory) - * [`--max_time`](#--max_time) - * [`--max_cpus`](#--max_cpus) - * [`--plaintext_email`](#--plaintext_email) - * [`--monochrome_logs`](#--monochrome_logs) - * [`--multiqc_config`](#--multiqc_config) - - - - -## Table of contents - - - -## Introduction +# Usage + +## Introduction Nextflow handles job submissions on SLURM or other environments, and supervises running the jobs. Thus the Nextflow process must run until the pipeline is finished. We recommend that you put the process running in the background through `screen` / `tmux` or similar tool. Alternatively you can run nextflow within a cluster job submitted your job scheduler. @@ -79,7 +10,7 @@ It is recommended to limit the Nextflow Java virtual machines memory. We recomme NXF_OPTS='-Xms1g -Xmx4g' ``` -## Running the pipeline +## Running the pipeline The typical command for running the pipeline is as follows: @@ -98,7 +29,7 @@ results # Finished results (configurable, see below) # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` -### Updating the pipeline +### Updating the pipeline When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: @@ -106,7 +37,7 @@ When you run the above command, Nextflow automatically pulls the pipeline code f nextflow pull nf-core/coproid ``` -### Reproducibility +### Reproducibility It's a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. @@ -114,9 +45,9 @@ First, go to the [nf-core/coproid releases page](https://github.com/nf-core/copr This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. -## Main arguments +## Main arguments -### `-profile` +### `-profile` Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. Note that multiple profiles can be loaded, for example: `-profile docker` - the order of arguments is important! @@ -137,7 +68,7 @@ If `-profile` is not specified at all the pipeline will be run locally and expec - A profile with a complete configuration for automated testing - Includes links to test data so needs no other parameters -### `--reads` +### `--reads` Use this to specify the location of your input FastQ files. For example: @@ -153,7 +84,7 @@ Please note the following requirements: If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz` -### `--singleEnd` +### `--singleEnd` By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--singleEnd` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--reads`. For example: @@ -163,23 +94,23 @@ By default, the pipeline expects paired-end data. If you have single-end data, y It is not possible to run a mixture of single-end and paired-end files in one run. -### `--name1` +### `--name1` Name of the first candidate species. Example : `"Homo_sapiens"` -### `--name2` +### `--name2` Name of the second candidate species. Example : `"Canis_familiaris"` -### `--krakenDB` +### `--krakenDB` Path to Path to Kraken2 MiniKraken2_v2_8GB Database. Can be downloaded [here](https://ccb.jhu.edu/software/kraken2/dl/old/minikraken2_v2_8GB.tgz) -## Reference genomes +## Reference genomes The pipeline config files come bundled with paths to the illumina iGenomes reference index files. If running with docker or AWS, the configuration is set up to use the [AWS-iGenomes](https://ewels.github.io/AWS-iGenomes/) resource. -### `--genome1` (using iGenomes) +### `--genome1` (using iGenomes) There are 31 different species supported in the iGenomes references. To run the pipeline, you must specify which to use with the `--genome` flag. @@ -244,7 +175,7 @@ params { } ``` -### `--fasta1` +### `--fasta1` If you prefer, you can specify the full path to your reference genome when you run the pipeline: @@ -252,7 +183,7 @@ If you prefer, you can specify the full path to your reference genome when you r --fasta1 'path/to/fasta/reference.fa' ``` -### `--fasta2` +### `--fasta2` If you prefer, you can specify the full path to your reference genome when you run the pipeline: @@ -260,7 +191,7 @@ If you prefer, you can specify the full path to your reference genome when you r --fasta2 'path/to/fasta/reference.fa' ``` -### `--genome2` (using iGenomes) +### `--genome2` (using iGenomes) Name of iGenomes reference for candidate organism 3. Must be provided if fasta2 is not provided @@ -268,13 +199,13 @@ Name of iGenomes reference for candidate organism 3. Must be provided if fasta2 --genome2 'CanFam3.1' ``` -### `--igenomesIgnore` +### `--igenomesIgnore` Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`. -## Settings +## Settings -### `--adna` +### `--adna` Specified if data is modern (false) or ancient DNA (true). Default = true @@ -288,7 +219,7 @@ or --adna false ``` -### `--phred` +### `--phred` Specifies the fastq quality encoding (33 | 64). Defaults to 33 @@ -302,7 +233,7 @@ or --phred 64 ``` -### `--collapse` +### `--collapse` Specifies if AdapterRemoval should merge the paired-end sequences or not. Default = true @@ -316,7 +247,7 @@ or --collapse false ``` -### `--identity` +### `--identity` Identity threshold to retain read alignment. Default = 0.95 @@ -324,7 +255,7 @@ Identity threshold to retain read alignment. Default = 0.95 --identity 0.95 ``` -### `--pmdscore` +### `--pmdscore` Minimum PMDscore to retain read alignment. Default = 3 @@ -332,7 +263,7 @@ Minimum PMDscore to retain read alignment. Default = 3 --pmdscore 3 ``` -### `--library` +### `--library` DNA preparation library type ( classic | UDGhalf). Default = classic @@ -346,7 +277,7 @@ or --library UDGhalf ``` -### `--bowtie` +### `--bowtie` Bowtie settings for sensivity (very-fast | very-sensitive). Default = very-sensitive @@ -360,7 +291,7 @@ or --bowtie very-sensitive ``` -### `--minKraken` +### `--minKraken` Minimum number of Kraken hits per Taxonomy ID to report. Default = 50 @@ -368,7 +299,7 @@ Minimum number of Kraken hits per Taxonomy ID to report. Default = 50 --minKraken 50 ``` -### `--endo1` +### `--endo1` Proportion of Endogenous DNA in organism 1 target microbiome. Must be between 0 and 1. Default = 0.01 @@ -376,7 +307,7 @@ Proportion of Endogenous DNA in organism 1 target microbiome. Must be between 0 --endo1 0.01 ``` -### `--endo2` +### `--endo2` Proportion of Endogenous DNA in organism 2 target microbiome. Must be between 0 and 1. Default = 0.01 @@ -384,7 +315,7 @@ Proportion of Endogenous DNA in organism 2 target microbiome. Must be between 0 --endo2 0.01 ``` -### `--endo3` +### `--endo3` Proportion of Endogenous DNA in organism 3 target microbiome. Must be between 0 and 1. Default = 0.01 @@ -392,13 +323,13 @@ Proportion of Endogenous DNA in organism 3 target microbiome. Must be between 0 --endo3 0.01 ``` -## Other coproID parameters +## Other coproID parameters -### `--name3` +### `--name3` Name of candidate 1. Example: "Sus_scrofa" -### `--fasta3` +### `--fasta3` Path to canidate organism 3 genome fasta file (must be surrounded with quotes). Must be provided if ### \`genome3 is not provided @@ -406,7 +337,7 @@ Path to canidate organism 3 genome fasta file (must be surrounded with quotes). --fasta3 'path/to/fasta/reference.fa' ``` -### `--genome3` (using iGenomes) +### `--genome3` (using iGenomes) Name of iGenomes reference for candidate organism 3. Must be provided if \`fasta3 is not provided @@ -414,7 +345,7 @@ Name of iGenomes reference for candidate organism 3. Must be provided if \`fasta --genome3 'Sscrofa10.2' ``` -### `--index1` +### `--index1` Path to Bowtie2 index genome candidate 2 Coprolite maker's genome @@ -422,7 +353,7 @@ Path to Bowtie2 index genome candidate 2 Coprolite maker's genome --index1 'path/to/bt_index/basename*' ``` -### `--index2` +### `--index2` Path to Bowtie2 index genome candidate 2 Coprolite maker's genome @@ -430,7 +361,7 @@ Path to Bowtie2 index genome candidate 2 Coprolite maker's genome --index2 'path/to/bt_index/basename*' ``` -### `--index3` +### `--index3` Path to Bowtie2 index genome candidate 3 Coprolite maker's genome @@ -438,13 +369,13 @@ Path to Bowtie2 index genome candidate 3 Coprolite maker's genome --index3 'path/to/bt_index/basename*' ``` -## Job resources +## Job resources -### Automatic resubmission +### Automatic resubmission Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. -### Custom resource requests +### Custom resource requests Wherever process-specific requirements are set in the pipeline, the default value can be changed by creating a custom config file. See the files hosted at [`nf-core/configs`](https://github.com/nf-core/configs/tree/master/conf) for examples. @@ -452,31 +383,31 @@ If you are likely to be running `nf-core` pipelines regularly it may be a good i If you have any questions or issues please send us a message on [Slack](https://nf-core-invite.herokuapp.com/). -## AWS Batch specific parameters +## AWS Batch specific parameters Running the pipeline on AWS Batch requires a couple of specific parameters to be set according to your AWS Batch configuration. Please use the `-awsbatch` profile and then specify all of the following parameters. -### `--awsqueue` +### `--awsqueue` The JobQueue that you intend to use on AWS Batch. -### `--awsregion` +### `--awsregion` The AWS region to run your job in. Default is set to `eu-west-1` but can be adjusted to your needs. Please make sure to also set the `-w/--work-dir` and `--outdir` parameters to a S3 storage bucket of your choice - you'll get an error message notifying you if you didn't. -## Other command line parameters +## Other command line parameters -### `--outdir` +### `--outdir` The output directory where the results will be saved. -### `--email` +### `--email` Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run. -### `-name` +### `-name` Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. @@ -484,7 +415,7 @@ This is used in the MultiQC report (if not default) and in the summary HTML / e- **NB:** Single hyphen (core Nextflow option) -### `-resume` +### `-resume` Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. @@ -492,7 +423,7 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U **NB:** Single hyphen (core Nextflow option) -### `-c` +### `-c` Specify the path to a specific config file (this is a core NextFlow command). @@ -500,7 +431,7 @@ Specify the path to a specific config file (this is a core NextFlow command). Note - you can use this to override pipeline defaults. -### `--custom_config_version` +### `--custom_config_version` Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default is set to `master`. @@ -509,7 +440,7 @@ Provide git commit id for custom Institutional configs hosted at `nf-core/config --custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96 ``` -### `--custom_config_base` +### `--custom_config_base` If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, @@ -530,29 +461,29 @@ nextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs > Note that the nf-core/tools helper package has a `download` command to download all required pipeline > files + singularity containers + institutional configs in one go for you, to make this process easier. -### `--max_memory` +### `--max_memory` Use to set a top-limit for the default memory requirement for each process. Should be a string in the format integer-unit. eg. `--max_memory '8.GB'` -### `--max_time` +### `--max_time` Use to set a top-limit for the default time requirement for each process. Should be a string in the format integer-unit. eg. `--max_time '2.h'` -### `--max_cpus` +### `--max_cpus` Use to set a top-limit for the default CPU requirement for each process. Should be a string in the format integer-unit. eg. `--max_cpus 1` -### `--plaintext_email` +### `--plaintext_email` Set to receive plain-text e-mails instead of HTML formatted. -### `--monochrome_logs` +### `--monochrome_logs` Set to disable colourful command line output and live life in monochrome. -### `--multiqc_config` +### `--multiqc_config` Specify a path to a custom MultiQC configuration file. From 560003fc9367cb65a7cf18b7c6fa42f829c0b2a2 Mon Sep 17 00:00:00 2001 From: maxibor Date: Mon, 29 Apr 2019 16:12:46 +0200 Subject: [PATCH 6/6] add readthedocs badge --- README.md | 1 + 1 file changed, 1 insertion(+) diff --git a/README.md b/README.md index 4f2095e..78a0cce 100644 --- a/README.md +++ b/README.md @@ -2,6 +2,7 @@ [![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A50.32.0-brightgreen.svg)](https://www.nextflow.io/) [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](http://bioconda.github.io/) [![Docker](https://img.shields.io/docker/automated/nfcore/coproid.svg)](https://hub.docker.com/r/nfcore/coproid) +[![Documentation Status](https://readthedocs.org/projects/coproid/badge/?version=latest)](https://coproid.readthedocs.io/en/latest/?badge=latest) # ![logo_coproid](assets/img/coproid_logo_small.jpg) ![logo_nf_core](assets/img/coproID_nf-core_logo_small.png)