From 7b289891945c61351b822d12c155662a41ca31fb Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 18 Mar 2024 11:00:16 +0100 Subject: [PATCH 01/80] bumpversion2.1.0dev --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index b3a8c6c8..b8bb11a1 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -3,9 +3,9 @@ custom_logo_url: https://github.com/nf-core/raredisease/ custom_logo_title: "nf-core/raredisease" report_comment: > - This report has been generated by the nf-core/raredisease + This report has been generated by the nf-core/raredisease analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-raredisease-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index b3f5e3e2..5065d2d9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -293,7 +293,7 @@ manifest { description = """call and score variants from WGS/WES of rare disease patients""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.0.0' + version = '2.1.0dev' doi = '' } From 45eb7d5c230f852d29d0461adadad068a0350fcf Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 18 Mar 2024 14:58:56 +0100 Subject: [PATCH 02/80] update changelog --- CHANGELOG.md | 19 +++++++++++++++++++ 1 file changed, 19 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index f676b8d9..b5243d01 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,25 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## 2.1.0 - Obelix [xxxx-xx-xx] + +### `Added` + +### `Changed` + +### `Fixed` + +### Parameters + +| Old parameter | New parameter | +| ------------- | ------------- | + +:::note +Parameter has been updated if both old and new parameter information is present. +Parameter has been added if just the new parameter information is present. +Parameter has been removed if new parameter information isn't present. +::: + ## 2.0.0 - Asterix [2024-03-18] ### `Added` From 701cae8e5c1fa0ac4e13eca35a98ef37ed5d5111 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 11 Apr 2024 13:54:07 +0200 Subject: [PATCH 03/80] patch --- conf/base.config | 6 +- conf/modules/align.config | 45 ++- conf/modules/align_MT.config | 60 ++-- conf/modules/align_bwa_bwamem2.config | 79 +++-- conf/modules/align_sentieon.config | 42 ++- conf/modules/annotate_consequence_pli.config | 71 ++--- conf/modules/annotate_genome_snvs.config | 196 ++++++------- conf/modules/annotate_mobile_elements.config | 7 - conf/modules/annotate_mt_snvs.config | 3 - .../annotate_structural_variants.config | 88 +++--- conf/modules/call_mobile_elements.config | 6 - conf/modules/call_repeat_expansions.config | 3 - conf/modules/call_snv.config | 6 - conf/modules/call_snv_sentieon.config | 62 ++-- conf/modules/call_structural_variants.config | 6 - conf/modules/call_sv_MT.config | 16 +- conf/modules/call_sv_germlinecnvcaller.config | 35 +-- conf/modules/generate_clinical_set.config | 6 +- conf/modules/generate_cytosure_files.config | 63 ++-- conf/modules/gens.config | 33 +-- conf/modules/prepare_references.config | 12 - conf/modules/rank_variants.config | 277 ++++++++---------- conf/modules/raredisease.config | 14 +- conf/test.config | 1 - lib/CustomFunctions.groovy | 12 - modules/local/create_hgncids_file.nf | 61 ++++ subworkflows/local/generate_clinical_set.nf | 8 +- workflows/raredisease.nf | 11 +- 28 files changed, 565 insertions(+), 664 deletions(-) create mode 100644 modules/local/create_hgncids_file.nf diff --git a/conf/base.config b/conf/base.config index 3f84e832..c65c634b 100644 --- a/conf/base.config +++ b/conf/base.config @@ -57,7 +57,9 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false + withName: 'NFCORE_RAREDISEASE:RAREDISEASE:.*' { + publishDir = [ + enabled: false + ] } } diff --git a/conf/modules/align.config b/conf/modules/align.config index 29fda646..84f4a36a 100644 --- a/conf/modules/align.config +++ b/conf/modules/align.config @@ -12,36 +12,27 @@ */ process{ - withName: '.*ALIGN:.*' { + + withName: '.*ALIGN:FASTP' { + ext.args = { [ + params.min_trimmed_length ? "--length_required ${params.min_trimmed_length}" : '', + '--correction', + '--overrepresentation_analysis' + ].join(' ').trim() } publishDir = [ - enabled: false + path: { "${params.outdir}/trimming" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } - if (!params.skip_fastp) { - withName: '.*ALIGN:FASTP' { - ext.args = { [ - params.min_trimmed_length ? "--length_required ${params.min_trimmed_length}" : '', - '--correction', - '--overrepresentation_analysis' - ].join(' ').trim() } - publishDir = [ - path: { "${params.outdir}/trimming" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - } - - if (params.save_mapped_as_cram) { - withName: '.*ALIGN:SAMTOOLS_VIEW' { - ext.args = { '--output-fmt cram --write-index' } - ext.prefix = { "${meta.id}_sorted_md" } - publishDir = [ - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ALIGN:SAMTOOLS_VIEW' { + ext.args = { '--output-fmt cram --write-index' } + ext.prefix = { "${meta.id}_sorted_md" } + publishDir = [ + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index 5ffec1c0..1bd88f14 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -17,28 +17,22 @@ process { - if (params.aligner.equals("bwamem2")) { - withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}_sorted" } - } + withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}_sorted" } } - if (params.aligner.equals("bwa")) { - withName: '.*ALIGN_MT:BWA_MEM_MT' { - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}_sorted" } - } + withName: '.*ALIGN_MT:BWA_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}_sorted" } } - if (params.aligner.equals("sentieon")) { - withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { - ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes"))} - ext.prefix = { "${meta.id}_sorted" } - } + withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { + ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes"))} + ext.prefix = { "${meta.id}_sorted" } } withName: '.*ALIGN_MT:GATK4_MERGEBAMALIGNMENT_MT' { @@ -74,28 +68,22 @@ process { process { - if (params.aligner.equals("bwamem2")) { - withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}_sorted_shifted" } - } + withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}_sorted_shifted" } } - if (params.aligner.equals("sentieon")) { - withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { - ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes"))} - ext.prefix = { "${meta.id}_sorted_shifted" } - } + withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { + ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes"))} + ext.prefix = { "${meta.id}_sorted_shifted" } } - if (params.aligner.equals("bwa")) { - withName: '.*ALIGN_MT_SHIFT:BWA_MEM_MT' { - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } - ext.prefix = { "${meta.id}_sorted_shifted" } - } + withName: '.*ALIGN_MT_SHIFT:BWA_MEM_MT' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.when = { !(params.analysis_type.equals("wes")) } + ext.prefix = { "${meta.id}_sorted_shifted" } } withName: '.*ALIGN_MT_SHIFT:GATK4_MERGEBAMALIGNMENT_MT' { diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index e91360fc..f0725de9 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -17,46 +17,43 @@ process { - if (params.aligner.equals("bwamem2") || params.aligner.equals("bwa")) { - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM2_MEM' { - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.prefix = { "${meta.id}_sorted" } - ext.when = { params.aligner.equals("bwamem2") } - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { - ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.prefix = { "${meta.id}_sorted" } - ext.when = { params.aligner.equals("bwa") } - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_STATS' { - ext.args = '-s --remove-overlaps' - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_MERGE' { - ext.prefix = { "${meta.id}_sorted_merged" } - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:MARKDUPLICATES' { - ext.args = "--TMP_DIR ." - ext.prefix = { "${meta.id}_sorted_md" } - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM2_MEM' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.prefix = { "${meta.id}_sorted" } + ext.when = { params.aligner.equals("bwamem2") } + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { + ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.prefix = { "${meta.id}_sorted" } + ext.when = { params.aligner.equals("bwa") } + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_STATS' { + ext.args = '-s --remove-overlaps' + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_MERGE' { + ext.prefix = { "${meta.id}_sorted_merged" } + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:MARKDUPLICATES' { + ext.args = "--TMP_DIR ." + ext.prefix = { "${meta.id}_sorted_md" } + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/align_sentieon.config b/conf/modules/align_sentieon.config index d5156e58..f3fb359f 100644 --- a/conf/modules/align_sentieon.config +++ b/conf/modules/align_sentieon.config @@ -17,30 +17,28 @@ process { - if (params.aligner.equals("sentieon")) { - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_BWAMEM' { - ext.args = { "-M -K 10000000 -R ${meta.read_group} " } - ext.prefix = { "${meta.id}.sorted" } - } + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_BWAMEM' { + ext.args = { "-M -K 10000000 -R ${meta.read_group} " } + ext.prefix = { "${meta.id}.sorted" } + } - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DATAMETRICS' { - ext.prefix = { "${meta.id}_datametrics" } - } + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DATAMETRICS' { + ext.prefix = { "${meta.id}_datametrics" } + } - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_READWRITER' { - ext.prefix = { "${meta.id}_merged" } - } + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_READWRITER' { + ext.prefix = { "${meta.id}_merged" } + } - withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DEDUP' { - ext.args = { $params.rmdup ? "--rmdup" : '' } - ext.prefix = { "${meta.id}_dedup" } - ext.suffix = ".bam" - publishDir = [ - enabled: !params.save_mapped_as_cram, - path: { "${params.outdir}/alignment" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ALIGN:ALIGN_SENTIEON:SENTIEON_DEDUP' { + ext.args = { $params.rmdup ? "--rmdup" : '' } + ext.prefix = { "${meta.id}_dedup" } + ext.suffix = ".bam" + publishDir = [ + enabled: !params.save_mapped_as_cram, + path: { "${params.outdir}/alignment" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/annotate_consequence_pli.config b/conf/modules/annotate_consequence_pli.config index e9451357..6cda707f 100644 --- a/conf/modules/annotate_consequence_pli.config +++ b/conf/modules/annotate_consequence_pli.config @@ -13,69 +13,46 @@ process { - if (!params.skip_sv_annotation) { - withName: '.*ANN_CSQ_PLI_SV:.*' { - publishDir = [ - enabled: false - ] - } - withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_sv_csq_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_sv_csq_${meta.set}" } + } - withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_sv_csq_pli_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_SV:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_sv_csq_pli_${meta.set}" } + } - withName: '.*ANN_CSQ_PLI_SV:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_sv_csq_pli_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_SV:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_sv_csq_pli_${meta.set}" } } } process { - if (!params.skip_snv_annotation) { - withName: '.*ANN_CSQ_PLI_SNV:.*' { - ext.when = !params.skip_snv_annotation - publishDir = [ - enabled: false - ] - } - withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_snv_csq_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_snv_csq_${meta.set}" } + } - withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_snv_csq_pli_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_SNV:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_snv_csq_pli_${meta.set}" } + } - withName: '.*ANN_CSQ_PLI_SNV:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_snv_csq_pli_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_SNV:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_snv_csq_pli_${meta.set}" } } } process { - if (!params.skip_mt_annotation) { - withName: '.*ANN_CSQ_PLI_MT:.*' { - ext.when = !params.skip_mt_annotation - publishDir = [ - enabled: false - ] - } - withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_mt_csq_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_mt_csq_${meta.set}" } + } - withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_mt_csq_pli_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_MT:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_mt_csq_pli_${meta.set}" } + } - withName: '.*ANN_CSQ_PLI_MT:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_mt_csq_pli_${meta.set}" } - } + withName: '.*ANN_CSQ_PLI_MT:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_mt_csq_pli_${meta.set}" } } } diff --git a/conf/modules/annotate_genome_snvs.config b/conf/modules/annotate_genome_snvs.config index 768aeee3..60bcd259 100644 --- a/conf/modules/annotate_genome_snvs.config +++ b/conf/modules/annotate_genome_snvs.config @@ -17,104 +17,102 @@ process { - if (!params.skip_snv_annotation) { - withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_ROH' { - ext.args = { "--samples ${meta.probands.unique().first()} --skip-indels " } - ext.prefix = { "${meta.id}_roh" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:RHOCALL_ANNOTATE' { - ext.args = { "--v14 " } - } - - withName: '.*ANNOTATE_GENOME_SNVS:ZIP_TABIX_ROHCALL' { - ext.prefix = { "${input.simpleName}" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { - ext.prefix = { "${vcf.simpleName}_vcfanno" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:UPD_SITES' { - ext.prefix = { "${vcf.simpleName}_upd_sites" } - ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} sites"} - } - - withName: '.*ANNOTATE_GENOME_SNVS:UPD_REGIONS' { - ext.prefix = { "${vcf.simpleName}_upd_regions" } - ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} regions --min-size 5 --min-sites 1"} - ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } - } - - withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_SITES' { - ext.prefix = { "${sites.simpleName}_chromograph" } - ext.args = { "--euploid" } - tag = {"${meta7.id}"} - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_REGIONS' { - ext.prefix = { "${regions.simpleName}_chromograph" } - ext.args = { '--euploid' } - ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } - tag = {"${meta6.id}"} - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ANNOTATE_GENOME_SNVS:ZIP_TABIX_VCFANNO' { - ext.prefix = { "${input.simpleName}" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { - ext.prefix = { "${vcf.simpleName}_filter" } - ext.args = { '--output-type z --exclude "INFO/GNOMADAF > 0.70 | INFO/GNOMADAF_popmax > 0.70" ' } - } - - withName: '.*ANNOTATE_GENOME_SNVS:GATK4_SELECTVARIANTS' { - ext.prefix = { "${vcf.simpleName}_${intervals.simpleName}" } - } - - withName: '.*ANNOTATE_GENOME_SNVS:ENSEMBLVEP_SNV' { - ext.prefix = { "${meta.prefix}_vep_${meta.scatterid}" } - ext.args = { [ - '--dir_plugins vep_cache/Plugins', - '--plugin LoFtool,LoFtool_scores.txt', - '--plugin pLI,pLI_values.txt', - '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', - '--distance 5000', - '--buffer_size 20000', - '--format vcf --max_sv_size 248956422', - '--appris --biotype --cache --canonical --ccds --compress_output bgzip', - '--domains --exclude_predicted --force_overwrite', - '--hgvs --humdiv --no_progress --numbers', - '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl', - '--uniprot --vcf' - ].join(' ') } - } - - withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { - ext.prefix = { "${meta.prefix}_vep" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*ANNOTATE_GENOME_SNVS:TABIX_BCFTOOLS_CONCAT' { - publishDir = [ - path: { "${params.outdir}/annotate_snv/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_ROH' { + ext.args = { "--samples ${meta.probands.unique().first()} --skip-indels " } + ext.prefix = { "${meta.id}_roh" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:RHOCALL_ANNOTATE' { + ext.args = { "--v14 " } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ZIP_TABIX_ROHCALL' { + ext.prefix = { "${input.simpleName}" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:VCFANNO' { + ext.prefix = { "${vcf.simpleName}_vcfanno" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:UPD_SITES' { + ext.prefix = { "${vcf.simpleName}_upd_sites" } + ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} sites"} + } + + withName: '.*ANNOTATE_GENOME_SNVS:UPD_REGIONS' { + ext.prefix = { "${vcf.simpleName}_upd_regions" } + ext.args = {"--af-tag GNOMADAF --proband ${meta.upd_child} --mother ${meta.mother} --father ${meta.father} regions --min-size 5 --min-sites 1"} + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample']).size() >= 1) || workflow.stubRun } + } + + withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_SITES' { + ext.prefix = { "${sites.simpleName}_chromograph" } + ext.args = { "--euploid" } + tag = {"${meta7.id}"} + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_GENOME_SNVS:CHROMOGRAPH_REGIONS' { + ext.prefix = { "${regions.simpleName}_chromograph" } + ext.args = { '--euploid' } + ext.when = { !(workflow.profile.tokenize(',').intersect(['test', 'test_one_sample', 'test_sentieon']).size() >= 1) || workflow.stubRun } + tag = {"${meta6.id}"} + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_GENOME_SNVS:ZIP_TABIX_VCFANNO' { + ext.prefix = { "${input.simpleName}" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_VIEW' { + ext.prefix = { "${vcf.simpleName}_filter" } + ext.args = { '--output-type z --exclude "INFO/GNOMADAF > 0.70 | INFO/GNOMADAF_popmax > 0.70" ' } + } + + withName: '.*ANNOTATE_GENOME_SNVS:GATK4_SELECTVARIANTS' { + ext.prefix = { "${vcf.simpleName}_${intervals.simpleName}" } + } + + withName: '.*ANNOTATE_GENOME_SNVS:ENSEMBLVEP_SNV' { + ext.prefix = { "${meta.prefix}_vep_${meta.scatterid}" } + ext.args = { [ + '--dir_plugins vep_cache/Plugins', + '--plugin LoFtool,LoFtool_scores.txt', + '--plugin pLI,pLI_values.txt', + '--plugin SpliceAI,snv=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz,indel=spliceai_21_scores_raw_snv_-v1.3-.vcf.gz', + '--distance 5000', + '--buffer_size 20000', + '--format vcf --max_sv_size 248956422', + '--appris --biotype --cache --canonical --ccds --compress_output bgzip', + '--domains --exclude_predicted --force_overwrite', + '--hgvs --humdiv --no_progress --numbers', + '--merged --polyphen p --protein --offline --regulatory --sift p --symbol --tsl', + '--uniprot --vcf' + ].join(' ') } + } + + withName: '.*ANNOTATE_GENOME_SNVS:BCFTOOLS_CONCAT' { + ext.prefix = { "${meta.prefix}_vep" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_GENOME_SNVS:TABIX_BCFTOOLS_CONCAT' { + publishDir = [ + path: { "${params.outdir}/annotate_snv/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index bed2c0a7..13425a7a 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -17,13 +17,6 @@ process { - withName: '.*ANNOTATE_MOBILE_ELEMENTS:.*' { - ext.when = !params.skip_me_annotation - publishDir = [ - enabled: false - ] - } - withName: '.*ANNOTATE_MOBILE_ELEMENTS:SVDB_QUERY_DB' { ext.args = { [ '--bnd_distance 150', diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index 3e25c36f..8675aff7 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -51,9 +51,6 @@ process { withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { ext.prefix = { "${meta.prefix}_uncompressed" } ext.args = '--offline' - publishDir = [ - enabled: false - ] } withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { diff --git a/conf/modules/annotate_structural_variants.config b/conf/modules/annotate_structural_variants.config index f8666a82..07725c24 100644 --- a/conf/modules/annotate_structural_variants.config +++ b/conf/modules/annotate_structural_variants.config @@ -17,58 +17,50 @@ process { - if (!params.skip_sv_annotation) { - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:.*' { - publishDir = [ - enabled: false - ] - } - - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_BEDPE' { - ext.when = {!params.svdb_query_bedpedbs.equals(null)} - ext.prefix = { "${meta.id}_bedpedb" } - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_BEDPE' { + ext.when = {!params.svdb_query_bedpedbs.equals(null)} + ext.prefix = { "${meta.id}_bedpedb" } + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_DB' { - ext.when = {!params.svdb_query_dbs.equals(null)} - ext.prefix = { "${meta.id}_vcfdb" } - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:SVDB_QUERY_DB' { + ext.when = {!params.svdb_query_dbs.equals(null)} + ext.prefix = { "${meta.id}_vcfdb" } + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:PICARD_SORTVCF' { - ext.prefix = { "${meta.id}_svdbquery" } - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:PICARD_SORTVCF' { + ext.prefix = { "${meta.id}_svdbquery" } + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:BCFTOOLS_VIEW' { - ext.prefix = { "${meta.id}_view" } - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:BCFTOOLS_VIEW' { + ext.prefix = { "${meta.id}_view" } + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:ENSEMBLVEP_SV' { - ext.args = { [ - '--dir_cache vep_cache', - '--dir_plugins vep_cache/Plugins', - '--plugin pLI,pLI_values.txt', - '--appris --biotype --buffer_size 100 --canonical --cache --ccds', - '--compress_output bgzip --distance 5000 --domains', - '--exclude_predicted --force_overwrite --format vcf', - '--fork 4 --hgvs --humdiv --max_sv_size 248956422 --merged', - '--no_progress --numbers --per_gene --polyphen p', - '--protein --offline --regulatory --sift p', - '--symbol --tsl --uniprot --vcf' - ].join(' ') } - ext.prefix = { "${meta.id}_svdbquery_vep" } - publishDir = [ - path: { "${params.outdir}/annotate_sv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:ENSEMBLVEP_SV' { + ext.args = { [ + '--dir_cache vep_cache', + '--dir_plugins vep_cache/Plugins', + '--plugin pLI,pLI_values.txt', + '--appris --biotype --buffer_size 100 --canonical --cache --ccds', + '--compress_output bgzip --distance 5000 --domains', + '--exclude_predicted --force_overwrite --format vcf', + '--fork 4 --hgvs --humdiv --max_sv_size 248956422 --merged', + '--no_progress --numbers --per_gene --polyphen p', + '--protein --offline --regulatory --sift p', + '--symbol --tsl --uniprot --vcf' + ].join(' ') } + ext.prefix = { "${meta.id}_svdbquery_vep" } + publishDir = [ + path: { "${params.outdir}/annotate_sv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } - withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:TABIX_VEP' { - publishDir = [ - path: { "${params.outdir}/annotate_sv" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*ANNOTATE_STRUCTURAL_VARIANTS:TABIX_VEP' { + publishDir = [ + path: { "${params.outdir}/annotate_sv" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/call_mobile_elements.config b/conf/modules/call_mobile_elements.config index 0a5e4faf..fd6bf61c 100644 --- a/conf/modules/call_mobile_elements.config +++ b/conf/modules/call_mobile_elements.config @@ -13,12 +13,6 @@ process { - withName: '.*CALL_MOBILE_ELEMENTS:.*' { - publishDir = [ - enabled: false - ] - } - withName: '.*CALL_MOBILE_ELEMENTS:ME_SPLIT_ALIGNMENT' { ext.args = { [ '--output-fmt bam', diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 7fbcdc4c..743b2a31 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -18,9 +18,6 @@ process { withName: '.*CALL_REPEAT_EXPANSIONS:.*' { ext.when = { params.analysis_type != "wes" } - publishDir = [ - enabled: false - ] } withName: '.*CALL_REPEAT_EXPANSIONS:EXPANSIONHUNTER' { diff --git a/conf/modules/call_snv.config b/conf/modules/call_snv.config index 1170d515..1546df4b 100644 --- a/conf/modules/call_snv.config +++ b/conf/modules/call_snv.config @@ -13,12 +13,6 @@ process { - withName: '.*CALL_SNV:.*' { - publishDir = [ - enabled: false - ] - } - withName: '.*CALL_SNV:GATK4_SELECTVARIANTS' { ext.args = { "--exclude-intervals ${params.mito_name}" } ext.prefix = { "${meta.id}_snv" } diff --git a/conf/modules/call_snv_sentieon.config b/conf/modules/call_snv_sentieon.config index 83cedb9f..869aabc3 100644 --- a/conf/modules/call_snv_sentieon.config +++ b/conf/modules/call_snv_sentieon.config @@ -17,45 +17,43 @@ process { - if(params.variant_caller.equals("sentieon")) { - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNASCOPE' { - ext.prefix = { "${meta.id}_dnascope" } - ext.args2 = { - params.variant_type ? "--var_type ${params.variant_type}" : '' - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNASCOPE' { + ext.prefix = { "${meta.id}_dnascope" } + ext.args2 = { + params.variant_type ? "--var_type ${params.variant_type}" : '' } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNAMODELAPPLY' { - ext.prefix = { "${meta.id}_dnamodelapply" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SENTIEON_DNAMODELAPPLY' { + ext.prefix = { "${meta.id}_dnamodelapply" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_ONE' { - ext.args = "-s 'ML_FAIL' -i 'INFO/ML_PROB <= 0.95' -m x -Oz" - ext.prefix = { "${meta.id}_mlfiltered_0.95" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_ONE' { + ext.args = "-s 'ML_FAIL' -i 'INFO/ML_PROB <= 0.95' -m x -Oz" + ext.prefix = { "${meta.id}_mlfiltered_0.95" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_TWO' { - ext.args = "-i FILTER='\"PASS\"' -m x -Oz" - ext.prefix = { "${meta.id}_passed" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCF_FILTER_TWO' { + ext.args = "-i FILTER='\"PASS\"' -m x -Oz" + ext.prefix = { "${meta.id}_passed" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SPLIT_MULTIALLELICS_SEN' { - ext.args = '--output-type z --multiallelics -both' - ext.prefix = { "${meta.id}_split" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:SPLIT_MULTIALLELICS_SEN' { + ext.args = '--output-type z --multiallelics -both' + ext.prefix = { "${meta.id}_split" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:REMOVE_DUPLICATES_SEN' { - ext.args = '--output-type z --rm-dup none' - ext.prefix = { "${meta.id}_split_rmdup" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:REMOVE_DUPLICATES_SEN' { + ext.args = '--output-type z --rm-dup none' + ext.prefix = { "${meta.id}_split_rmdup" } + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:ADD_VARCALLER_TO_BED' { - ext.args2 = '-s 1 -b 2 -e 3' - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:ADD_VARCALLER_TO_BED' { + ext.args2 = '-s 1 -b 2 -e 3' + } - withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCFTOOLS_ANNOTATE' { - ext.args = "-c CHROM,FROM,TO,FOUND_IN --output-type z" - ext.prefix = { "${meta.id}_split_rmdup_info" } - } + withName: '.*CALL_SNV:CALL_SNV_SENTIEON:BCFTOOLS_ANNOTATE' { + ext.args = "-c CHROM,FROM,TO,FOUND_IN --output-type z" + ext.prefix = { "${meta.id}_split_rmdup_info" } } } diff --git a/conf/modules/call_structural_variants.config b/conf/modules/call_structural_variants.config index f8da7840..23e9325b 100644 --- a/conf/modules/call_structural_variants.config +++ b/conf/modules/call_structural_variants.config @@ -17,12 +17,6 @@ process { - withName: '.*CALL_STRUCTURAL_VARIANTS:.*' { - publishDir = [ - enabled: false - ] - } - withName: '.*CALL_STRUCTURAL_VARIANTS:SVDB_MERGE' { ext.prefix = {"${meta.id}_sv"} ext.args = '--pass_only --same_order' diff --git a/conf/modules/call_sv_MT.config b/conf/modules/call_sv_MT.config index 05f98e84..388fee33 100644 --- a/conf/modules/call_sv_MT.config +++ b/conf/modules/call_sv_MT.config @@ -27,15 +27,13 @@ process { ] } - if (!params.skip_eklipse){ - withName: '.*CALL_SV_MT:EKLIPSE' { - ext.args = "-tmp ." - publishDir = [ - path: { "${params.outdir}/call_sv/mitochondria" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*CALL_SV_MT:EKLIPSE' { + ext.args = "-tmp ." + publishDir = [ + path: { "${params.outdir}/call_sv/mitochondria" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/call_sv_germlinecnvcaller.config b/conf/modules/call_sv_germlinecnvcaller.config index 5f85c0f9..a63da3d3 100644 --- a/conf/modules/call_sv_germlinecnvcaller.config +++ b/conf/modules/call_sv_germlinecnvcaller.config @@ -17,30 +17,21 @@ process { - if (!params.skip_germlinecnvcaller) { - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER.*" { - publishDir = [ - enabled: false - ] - ext.when = !params.skip_germlinecnvcaller - } - - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_COLLECTREADCOUNTS" { - ext.args = "--format TSV --interval-merging-rule OVERLAPPING_ONLY" - } + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_COLLECTREADCOUNTS" { + ext.args = "--format TSV --interval-merging-rule OVERLAPPING_ONLY" + } - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_DETERMINEGERMLINECONTIGPLOIDY" { - ext.prefix = { "${meta.id}_ploidy" } - } + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_DETERMINEGERMLINECONTIGPLOIDY" { + ext.prefix = { "${meta.id}_ploidy" } + } - withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_GERMLINECNVCALLER" { - ext.args = "--run-mode CASE" - ext.prefix = { "${meta.id}_${model.simpleName}" } - } + withName: ".*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:GATK4_GERMLINECNVCALLER" { + ext.args = "--run-mode CASE" + ext.prefix = { "${meta.id}_${model.simpleName}" } + } - withName: '.*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:BCFTOOLS_VIEW' { - ext.prefix = { "${meta.id}_gatkcnv_segments_refiltered" } - ext.args = { '--output-type z --exclude "N_ALT = 0" ' } - } + withName: '.*CALL_STRUCTURAL_VARIANTS:CALL_SV_GERMLINECNVCALLER:BCFTOOLS_VIEW' { + ext.prefix = { "${meta.id}_gatkcnv_segments_refiltered" } + ext.args = { '--output-type z --exclude "N_ALT = 0" ' } } } diff --git a/conf/modules/generate_clinical_set.config b/conf/modules/generate_clinical_set.config index 4ab31579..75502110 100644 --- a/conf/modules/generate_clinical_set.config +++ b/conf/modules/generate_clinical_set.config @@ -21,7 +21,7 @@ process { withName: '.*:GENERATE_CLINICAL_SET_SNV:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_snv_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*:GENERATE_CLINICAL_SET_SNV:TABIX_BGZIP' { @@ -36,7 +36,7 @@ process { withName: '.*:GENERATE_CLINICAL_SET_SV:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${input.simpleName}_sv_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*:GENERATE_CLINICAL_SET_SV:TABIX_BGZIP' { @@ -51,7 +51,7 @@ process { withName: '.*:GENERATE_CLINICAL_SET_MT:ENSEMBLVEP_FILTERVEP' { ext.when = !params.skip_vep_filter ext.prefix = { "${meta.id}_mt_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } } withName: '.*:GENERATE_CLINICAL_SET_MT:TABIX_BGZIP' { diff --git a/conf/modules/generate_cytosure_files.config b/conf/modules/generate_cytosure_files.config index 1cd076f3..4cdb2f21 100644 --- a/conf/modules/generate_cytosure_files.config +++ b/conf/modules/generate_cytosure_files.config @@ -17,41 +17,38 @@ process { - if ( params.analysis_type != "wes" && !params.skip_vcf2cytosure ) { - - withName: '.*GENERATE_CYTOSURE_FILES:TIDDIT_COV_VCF2CYTOSURE' { - ext.args = { '-z 500' } - ext.prefix = { "${meta.id}_cov" } - } + withName: '.*GENERATE_CYTOSURE_FILES:TIDDIT_COV_VCF2CYTOSURE' { + ext.args = { '-z 500' } + ext.prefix = { "${meta.id}_cov" } + } - withName: '.*GENERATE_CYTOSURE_FILES:SPLIT_AND_FILTER_SV_VCF' { - ext.args = { [ - "--samples ${meta.id}", - '--output-type z', - "--exclude 'gnomad_svAF > 0.05'" - ].join(' ') } - ext.prefix = { "${meta.id}" } - } + withName: '.*GENERATE_CYTOSURE_FILES:SPLIT_AND_FILTER_SV_VCF' { + ext.args = { [ + "--samples ${meta.id}", + '--output-type z', + "--exclude 'gnomad_svAF > 0.05'" + ].join(' ') } + ext.prefix = { "${meta.id}" } + } - withName: '.*GENERATE_CYTOSURE_FILES:BCFTOOLS_REHEADER_SV_VCF' { - beforeScript = { "echo ${meta.custid} > ${meta.custid}.txt" } - ext.args = { "--samples ${meta.custid}.txt" } - ext.args2 = "--output-type v" - ext.prefix = { "${meta.custid}" } - } + withName: '.*GENERATE_CYTOSURE_FILES:BCFTOOLS_REHEADER_SV_VCF' { + beforeScript = { "echo ${meta.custid} > ${meta.custid}.txt" } + ext.args = { "--samples ${meta.custid}.txt" } + ext.args2 = "--output-type v" + ext.prefix = { "${meta.custid}" } + } - withName: '.*GENERATE_CYTOSURE_FILES:VCF2CYTOSURE' { - ext.args = { [ - meta.sex.equals(1) ? '--sex male' : '--sex female', - '--size 5000', - '--maxbnd 5000' - ].join(' ') } - ext.prefix = { "${meta.custid}" ? "${meta.custid}" : "${meta.id}" } - publishDir = [ - path: { "${params.outdir}/vcf2cytosure" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - ] - } + withName: '.*GENERATE_CYTOSURE_FILES:VCF2CYTOSURE' { + ext.args = { [ + meta.sex.equals(1) ? '--sex male' : '--sex female', + '--size 5000', + '--maxbnd 5000' + ].join(' ') } + ext.prefix = { "${meta.custid}" ? "${meta.custid}" : "${meta.id}" } + publishDir = [ + path: { "${params.outdir}/vcf2cytosure" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] } } diff --git a/conf/modules/gens.config b/conf/modules/gens.config index 6882775c..691cacd7 100644 --- a/conf/modules/gens.config +++ b/conf/modules/gens.config @@ -16,27 +16,20 @@ // process { - if (!params.skip_gens && params.analysis_type != "wes") { - withName: '.*GENS:.*' { - publishDir = [ - enabled: false - ] - } - withName: '.*GENS:COLLECTREADCOUNTS' { - ext.args = { [ - '--interval-merging-rule OVERLAPPING_ONLY', - '--format HDF5' - ].join(' ') } - } + withName: '.*GENS:COLLECTREADCOUNTS' { + ext.args = { [ + '--interval-merging-rule OVERLAPPING_ONLY', + '--format HDF5' + ].join(' ') } + } - withName: '.*GENS:GENS_GENERATE' { - ext.prefix = { "${meta.id}_gens" } - publishDir = [ - path: { "${params.outdir}/gens" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + withName: '.*GENS:GENS_GENERATE' { + ext.prefix = { "${meta.id}_gens" } + publishDir = [ + path: { "${params.outdir}/gens" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 3721d220..04416bcd 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -78,12 +78,6 @@ process { ext.when = { !(params.analysis_type == "wes")} } - withName: '.*PREPARE_REFERENCES:GET_CHROM_SIZES' { - publishDir = [ - enabled: false, - ] - } - withName: '.*PREPARE_REFERENCES:TABIX_DBSNP' { ext.when = {params.known_dbsnp && !params.known_dbsnp_tbi} } @@ -109,9 +103,6 @@ process { withName: '.*PREPARE_REFERENCES:GATK_ILT' { ext.when = { !params.target_bed.equals(null) } ext.args = { "--PADDING ${params.bait_padding} -SUBDIVISION_MODE INTERVAL_SUBDIVISION --SCATTER_COUNT 2" } - publishDir = [ - enabled: false - ] } withName: '.*PREPARE_REFERENCES:CAT_CAT_BAIT' { @@ -121,9 +112,6 @@ process { withName: '.*PREPARE_REFERENCES:UNTAR_VEP_CACHE' { ext.when = { (params.vep_cache && params.vep_cache.endsWith("tar.gz")) } - publishDir = [ - enabled: false - ] } withName: '.*PREPARE_REFERENCES:GATK_PREPROCESS_WGS' { diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index a17dee05..3d8f1804 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -16,56 +16,49 @@ // process { - if (!params.skip_sv_annotation) { - withName: '.*RANK_VARIANTS_SV:.*' { - publishDir = [ - enabled: false - ] - } - - withName: '.*RANK_VARIANTS_SV:GENMOD_ANNOTATE' { - ext.prefix = { "${meta.id}_sv_genmod_annotate_${meta.set}" } - ext.args = { [ - '--annotate_regions', - params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' - ].join(' ') } - } - - withName: '.*RANK_VARIANTS_SV:GENMOD_MODELS' { - ext.prefix = { "${meta.id}_sv_genmod_models_${meta.set}" } - ext.args = " --whole_gene " - } - - withName: '.*RANK_VARIANTS_SV:GENMOD_SCORE' { - ext.prefix = { "${meta.id}_sv_genmod_score_${meta.set}" } - ext.args = " --rank_results " - } - - withName: '.*RANK_VARIANTS_SV:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_sv_genmod_compound_${meta.set}" } - } - - withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { - ext.args = "--output-type z" - ext.prefix = { "${meta.id}_sv_ranked_${meta.set}" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*RANK_VARIANTS_SV:TABIX_BGZIP' { - ext.when = false - } - - withName: '.*RANK_VARIANTS_SV:TABIX_TABIX' { - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + + withName: '.*RANK_VARIANTS_SV:GENMOD_ANNOTATE' { + ext.prefix = { "${meta.id}_sv_genmod_annotate_${meta.set}" } + ext.args = { [ + '--annotate_regions', + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + ].join(' ') } + } + + withName: '.*RANK_VARIANTS_SV:GENMOD_MODELS' { + ext.prefix = { "${meta.id}_sv_genmod_models_${meta.set}" } + ext.args = " --whole_gene " + } + + withName: '.*RANK_VARIANTS_SV:GENMOD_SCORE' { + ext.prefix = { "${meta.id}_sv_genmod_score_${meta.set}" } + ext.args = " --rank_results " + } + + withName: '.*RANK_VARIANTS_SV:GENMOD_COMPOUND' { + ext.prefix = { "${meta.id}_sv_genmod_compound_${meta.set}" } + } + + withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { + ext.args = "--output-type z" + ext.prefix = { "${meta.id}_sv_ranked_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*RANK_VARIANTS_SV:TABIX_BGZIP' { + ext.when = false + } + + withName: '.*RANK_VARIANTS_SV:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } @@ -74,56 +67,48 @@ process { // process { - if (!params.skip_snv_annotation) { - withName: '.*RANK_VARIANTS_SNV:.*' { - ext.when = !params.skip_snv_annotation - publishDir = [ - enabled: false - ] - } - - withName: '.*RANK_VARIANTS_SNV:GENMOD_ANNOTATE' { - ext.prefix = { "${meta.id}_snv_genmod_annotate_${meta.set}" } - ext.args = { [ - '--annotate_regions', - params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' - ].join(' ') } - } - - withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { - ext.prefix = { "${meta.id}_snv_genmod_models_${meta.set}" } - ext.args = " --whole_gene " - } - - withName: '.*RANK_VARIANTS_SNV:GENMOD_SCORE' { - ext.prefix = { "${meta.id}_snv_genmod_score_${meta.set}" } - ext.args = " --rank_results " - } - - withName: '.*RANK_VARIANTS_SNV:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_snv_genmod_compound_${meta.set}" } - } - - withName: '.*RANK_VARIANTS_SNV:BCFTOOLS_SORT' { - ext.when = false - } - - withName: '.*RANK_VARIANTS_SNV:TABIX_BGZIP' { - ext.prefix = { "${meta.id}_snv_ranked_${meta.set}" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*RANK_VARIANTS_SNV:TABIX_TABIX' { - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + + withName: '.*RANK_VARIANTS_SNV:GENMOD_ANNOTATE' { + ext.prefix = { "${meta.id}_snv_genmod_annotate_${meta.set}" } + ext.args = { [ + '--annotate_regions', + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + ].join(' ') } + } + + withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { + ext.prefix = { "${meta.id}_snv_genmod_models_${meta.set}" } + ext.args = " --whole_gene " + } + + withName: '.*RANK_VARIANTS_SNV:GENMOD_SCORE' { + ext.prefix = { "${meta.id}_snv_genmod_score_${meta.set}" } + ext.args = " --rank_results " + } + + withName: '.*RANK_VARIANTS_SNV:GENMOD_COMPOUND' { + ext.prefix = { "${meta.id}_snv_genmod_compound_${meta.set}" } + } + + withName: '.*RANK_VARIANTS_SNV:BCFTOOLS_SORT' { + ext.when = false + } + + withName: '.*RANK_VARIANTS_SNV:TABIX_BGZIP' { + ext.prefix = { "${meta.id}_snv_ranked_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*RANK_VARIANTS_SNV:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } @@ -132,55 +117,47 @@ process { // process { - if (!params.skip_mt_annotation) { - withName: '.*RANK_VARIANTS_MT:.*' { - ext.when = !params.skip_mt_annotation - publishDir = [ - enabled: false - ] - } - - withName: '.*RANK_VARIANTS_MT:GENMOD_ANNOTATE' { - ext.prefix = { "${meta.id}_mt_genmod_annotate_${meta.set}" } - ext.args = { [ - '--annotate_regions', - params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' - ].join(' ') } - } - - withName: '.*RANK_VARIANTS_MT:GENMOD_MODELS' { - ext.prefix = { "${meta.id}_mt_genmod_models_${meta.set}" } - ext.args = " --whole_gene " - } - - withName: '.*RANK_VARIANTS_MT:GENMOD_SCORE' { - ext.prefix = { "${meta.id}_mt_genmod_score_${meta.set}" } - ext.args = " --rank_results " - } - - withName: '.*RANK_VARIANTS_MT:GENMOD_COMPOUND' { - ext.prefix = { "${meta.id}_mt_genmod_compound_${meta.set}" } - } - - withName: '.*RANK_VARIANTS_MT:BCFTOOLS_SORT' { - ext.when = false - } - - withName: '.*RANK_VARIANTS_MT:TABIX_BGZIP' { - ext.prefix = { "${meta.id}_mt_ranked_${meta.set}" } - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: '.*RANK_VARIANTS_MT:TABIX_TABIX' { - publishDir = [ - path: { "${params.outdir}/rank_and_filter" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } + + withName: '.*RANK_VARIANTS_MT:GENMOD_ANNOTATE' { + ext.prefix = { "${meta.id}_mt_genmod_annotate_${meta.set}" } + ext.args = { [ + '--annotate_regions', + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + ].join(' ') } + } + + withName: '.*RANK_VARIANTS_MT:GENMOD_MODELS' { + ext.prefix = { "${meta.id}_mt_genmod_models_${meta.set}" } + ext.args = " --whole_gene " + } + + withName: '.*RANK_VARIANTS_MT:GENMOD_SCORE' { + ext.prefix = { "${meta.id}_mt_genmod_score_${meta.set}" } + ext.args = " --rank_results " + } + + withName: '.*RANK_VARIANTS_MT:GENMOD_COMPOUND' { + ext.prefix = { "${meta.id}_mt_genmod_compound_${meta.set}" } + } + + withName: '.*RANK_VARIANTS_MT:BCFTOOLS_SORT' { + ext.when = false + } + + withName: '.*RANK_VARIANTS_MT:TABIX_BGZIP' { + ext.prefix = { "${meta.id}_mt_ranked_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*RANK_VARIANTS_MT:TABIX_TABIX' { + publishDir = [ + path: { "${params.outdir}/rank_and_filter" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } } diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index a16e6e0c..a4f1bbac 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -32,14 +32,12 @@ process { process { - if (!params.skip_fastqc) { - withName: '.*RAREDISEASE:FASTQC' { - ext.args = '--quiet' - publishDir = [ - path: { "${params.outdir}/fastqc/${meta.id}" }, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - ] - } + withName: '.*RAREDISEASE:FASTQC' { + ext.args = '--quiet' + publishDir = [ + path: { "${params.outdir}/fastqc/${meta.id}" }, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] } } diff --git a/conf/test.config b/conf/test.config index 5301f96b..adc70962 100644 --- a/conf/test.config +++ b/conf/test.config @@ -60,7 +60,6 @@ params { variant_consequences_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" variant_consequences_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" - vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } diff --git a/lib/CustomFunctions.groovy b/lib/CustomFunctions.groovy index 9ed0a9c0..0ff3eadd 100644 --- a/lib/CustomFunctions.groovy +++ b/lib/CustomFunctions.groovy @@ -51,16 +51,4 @@ class CustomFunctions { return case_info } - // create hgnc list - public static ArrayList parseHgncIds(List text) { - def ids = [] - def lines = text[0].tokenize("\n") - for(int i = 0; i versions.yml + "${task.process}": + create_hgncids_file: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ + + stub: + """ + python3 < versions.yml + "${task.process}": + create_hgncids_file: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} diff --git a/subworkflows/local/generate_clinical_set.nf b/subworkflows/local/generate_clinical_set.nf index 87250ff9..3e71e6b0 100644 --- a/subworkflows/local/generate_clinical_set.nf +++ b/subworkflows/local/generate_clinical_set.nf @@ -15,19 +15,17 @@ workflow GENERATE_CLINICAL_SET { ch_versions = Channel.empty() ch_vcf - .combine(ch_hgnc_ids) - .multiMap { meta, vcf, ids -> - clinical: [ meta + [ set: "clinical", hgnc_ids:ids ], vcf ] + .multiMap { meta, vcf -> + clinical: [ meta + [ set: "clinical" ], vcf ] research: [ meta + [ set: "research" ], vcf ] } .set { ch_clin_research_vcf } ENSEMBLVEP_FILTERVEP( ch_clin_research_vcf.clinical, - [] + ch_hgnc_ids ) .output - .map {meta, vcf -> [ meta - meta.subMap('hgnc_ids'), vcf ]} .set { ch_filtervep_out } TABIX_BGZIP( ch_filtervep_out ) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 96debe46..6553281f 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -112,6 +112,7 @@ include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/mai include { RENAME_ALIGN_FILES as RENAME_BAM_FOR_SMNCALLER } from '../modules/local/rename_align_files' include { RENAME_ALIGN_FILES as RENAME_BAI_FOR_SMNCALLER } from '../modules/local/rename_align_files' +include { CREATE_HGNCIDS_FILE } from '../modules/local/create_hgncids_file' // // SUBWORKFLOWS @@ -285,9 +286,9 @@ workflow RAREDISEASE { : ( params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : Channel.value([]) ) ch_vep_extra_files_unsplit = params.vep_plugin_files ? Channel.fromPath(params.vep_plugin_files).collect() : Channel.value([]) - ch_vep_filters_std_fmt = params.vep_filters ? Channel.fromPath(params.vep_filters).splitCsv().collect() + ch_vep_filters_std_fmt = params.vep_filters ? Channel.fromPath(params.vep_filters).map { it -> [[id:'standard'],it]}.collect() : Channel.empty() - ch_vep_filters_scout_fmt = params.vep_filters_scout_fmt ? Channel.fromPath(params.vep_filters_scout_fmt).collect() + ch_vep_filters_scout_fmt = params.vep_filters_scout_fmt ? Channel.fromPath(params.vep_filters_scout_fmt).map { it -> [[id:'scout'],it]}.collect() : Channel.empty() ch_versions = ch_versions.mix(ch_references.versions) @@ -315,9 +316,11 @@ workflow RAREDISEASE { // Read and store hgnc ids in a channel ch_vep_filters_scout_fmt - .map { it -> CustomFunctions.parseHgncIds(it.text) } .mix (ch_vep_filters_std_fmt) - .toList() + .set {ch_vep_filters} + + CREATE_HGNCIDS_FILE(ch_vep_filters) + .txt .set {ch_hgnc_ids} // Input QC From 7b3c42ef5e67f63a6cc483047b1a4b663fd413a0 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 12 Apr 2024 00:09:00 +0200 Subject: [PATCH 04/80] update output --- modules/local/create_hgncids_file.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/create_hgncids_file.nf b/modules/local/create_hgncids_file.nf index 439e7c4d..a6e1abdb 100644 --- a/modules/local/create_hgncids_file.nf +++ b/modules/local/create_hgncids_file.nf @@ -11,8 +11,8 @@ process CREATE_HGNCIDS_FILE { tuple val(meta), path(input) output: - tuple val(meta), path("*_reformatted.txt"), emit: txt - path "versions.yml" , emit: versions + path("*_reformatted.txt"), emit: txt + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when From 82ad9021c8a763cb0213a53b4092135d830d10b0 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 12 Apr 2024 13:50:39 +0200 Subject: [PATCH 05/80] update me vep --- conf/modules/annotate_consequence_pli.config | 20 ++++++++++++++ conf/modules/annotate_mobile_elements.config | 27 ------------------- conf/modules/generate_clinical_set.config | 14 ++++++++++ .../local/annotate_mobile_elements.nf | 18 +------------ workflows/raredisease.nf | 16 ++++++++++- 5 files changed, 50 insertions(+), 45 deletions(-) diff --git a/conf/modules/annotate_consequence_pli.config b/conf/modules/annotate_consequence_pli.config index 6cda707f..21e2e835 100644 --- a/conf/modules/annotate_consequence_pli.config +++ b/conf/modules/annotate_consequence_pli.config @@ -56,3 +56,23 @@ process { ext.prefix = { "${meta.id}_mt_csq_pli_${meta.set}" } } } + +process { + + withName: '.*ANN_CSQ_PLI_ME:ADD_MOST_SEVERE_CSQ' { + ext.prefix = { "${meta.id}_me_csq_${meta.set}" } + } + + withName: '.*ANN_CSQ_PLI_ME:ADD_MOST_SEVERE_PLI' { + ext.prefix = { "${meta.id}_me_pli_${meta.set}" } + } + + withName: '.*ANN_CSQ_PLI_ME:TABIX_BGZIPTABIX' { + ext.prefix = { "${meta.id}_me_annotated_${meta.set}" } + publishDir = [ + path: { "${params.outdir}/annotate_mobile_elements" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } +} diff --git a/conf/modules/annotate_mobile_elements.config b/conf/modules/annotate_mobile_elements.config index 13425a7a..7d001727 100644 --- a/conf/modules/annotate_mobile_elements.config +++ b/conf/modules/annotate_mobile_elements.config @@ -51,31 +51,4 @@ process { ext.prefix = { "${meta.id}_filter" } } - withName: '.*:ANNOTATE_MOBILE_ELEMENTS:GENERATE_CLINICAL_SET_ME:ENSEMBLVEP_FILTERVEP' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_me_${meta.set}" } - ext.args = { "--filter \"HGNC_ID in ${meta.hgnc_ids.join(',')}\"" } - } - - withName: '.*:ANNOTATE_MOBILE_ELEMENTS:GENERATE_CLINICAL_SET_ME:TABIX_BGZIP' { - ext.when = !params.skip_vep_filter - ext.prefix = { "${meta.id}_me_${meta.set}" } - } - - withName: '.*ANNOTATE_MOBILE_ELEMENTS:ANNOTATE_CSQ_PLI_ME:ADD_MOST_SEVERE_CSQ' { - ext.prefix = { "${meta.id}_me_csq_${meta.set}" } - } - - withName: '.*ANNOTATE_MOBILE_ELEMENTS:ANNOTATE_CSQ_PLI_ME:ADD_MOST_SEVERE_PLI' { - ext.prefix = { "${meta.id}_me_pli_${meta.set}" } - } - - withName: '.*ANNOTATE_MOBILE_ELEMENTS:ANNOTATE_CSQ_PLI_ME:TABIX_BGZIPTABIX' { - ext.prefix = { "${meta.id}_me_annotated_${meta.set}" } - publishDir = [ - path: { "${params.outdir}/annotate_mobile_elements" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } } diff --git a/conf/modules/generate_clinical_set.config b/conf/modules/generate_clinical_set.config index 75502110..de4d1b27 100644 --- a/conf/modules/generate_clinical_set.config +++ b/conf/modules/generate_clinical_set.config @@ -59,3 +59,17 @@ process { ext.prefix = { "${meta.id}_mt_${meta.set}" } } } + +// ME variants +process{ + withName: '.*:GENERATE_CLINICAL_SET_ME:ENSEMBLVEP_FILTERVEP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_me_${meta.set}" } + ext.args = { "--filter \"HGNC_ID in ${feature_file}\"" } + } + + withName: '.*:GENERATE_CLINICAL_SET_ME:TABIX_BGZIP' { + ext.when = !params.skip_vep_filter + ext.prefix = { "${meta.id}_me_${meta.set}" } + } +} diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index 0d22cd80..cb6087a7 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -7,8 +7,6 @@ include { ENSEMBLVEP_VEP as ENSEMBLVEP_ME } from '../../modules/nf-core/en include { PICARD_SORTVCF } from '../../modules/nf-core/picard/sortvcf/main' include { SVDB_QUERY as SVDB_QUERY_DB } from '../../modules/nf-core/svdb/query/main' -include { ANNOTATE_CSQ_PLI as ANNOTATE_CSQ_PLI_ME } from '../../subworkflows/local/annotate_consequence_pli.nf' -include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_ME } from '../../subworkflows/local/generate_clinical_set.nf' workflow ANNOTATE_MOBILE_ELEMENTS { @@ -19,7 +17,6 @@ workflow ANNOTATE_MOBILE_ELEMENTS { ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] ch_vep_cache // channel: [mandatory] [ path(cache) ] ch_variant_consequences // channel: [mandatory] [ path(consequences) ] - ch_hgnc_ids // channel: [mandatory] [ val(hgnc_ids) ] val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] default: 107 ch_vep_extra_files // channel: [mandatory] [ path(files) ] @@ -76,25 +73,12 @@ workflow ANNOTATE_MOBILE_ELEMENTS { BCFTOOLS_VIEW_FILTER( ch_bcftools_filter_input, [], [], [] ) - GENERATE_CLINICAL_SET_ME( - BCFTOOLS_VIEW_FILTER.out.vcf, - ch_hgnc_ids - ) - - ANNOTATE_CSQ_PLI_ME( - GENERATE_CLINICAL_SET_ME.out.vcf, - ch_variant_consequences - ) - ch_versions = ch_versions.mix( SVDB_QUERY_DB.out.versions ) ch_versions = ch_versions.mix( PICARD_SORTVCF.out.versions ) ch_versions = ch_versions.mix( ENSEMBLVEP_ME.out.versions ) ch_versions = ch_versions.mix( BCFTOOLS_VIEW_FILTER.out.versions ) - ch_versions = ch_versions.mix( GENERATE_CLINICAL_SET_ME.out.versions ) - ch_versions = ch_versions.mix( ANNOTATE_CSQ_PLI_ME.out.versions ) emit: - vcf = ANNOTATE_CSQ_PLI_ME.out.vcf_ann // channel: [ val(meta), path(vcf) ] - tbi = ANNOTATE_CSQ_PLI_ME.out.tbi_ann // channel: [ val(meta), path(tbi) ] + vcf = BCFTOOLS_VIEW_FILTER.out.vcf // channel: [ val(meta), path(vcf) ] versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 6553281f..84f68eb6 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -119,6 +119,7 @@ include { CREATE_HGNCIDS_FILE } from '../modules/loca // include { ALIGN } from '../subworkflows/local/align' +include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_ME } from '../subworkflows/local/annotate_consequence_pli.nf' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_MT } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SNV } from '../subworkflows/local/annotate_consequence_pli' include { ANNOTATE_CSQ_PLI as ANN_CSQ_PLI_SV } from '../subworkflows/local/annotate_consequence_pli' @@ -130,6 +131,7 @@ include { CALL_MOBILE_ELEMENTS } from '../subworkf include { CALL_REPEAT_EXPANSIONS } from '../subworkflows/local/call_repeat_expansions' include { CALL_SNV } from '../subworkflows/local/call_snv' include { CALL_STRUCTURAL_VARIANTS } from '../subworkflows/local/call_structural_variants' +include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_ME } from '../subworkflows/local/generate_clinical_set.nf' include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_MT } from '../subworkflows/local/generate_clinical_set' include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_SNV } from '../subworkflows/local/generate_clinical_set' include { GENERATE_CLINICAL_SET as GENERATE_CLINICAL_SET_SV } from '../subworkflows/local/generate_clinical_set' @@ -668,12 +670,24 @@ workflow RAREDISEASE { ch_genome_dictionary, ch_vep_cache, ch_variant_consequences_sv, - ch_hgnc_ids, params.genome, params.vep_cache_version, ch_vep_extra_files ) ch_versions = ch_versions.mix(ANNOTATE_MOBILE_ELEMENTS.out.versions) + + GENERATE_CLINICAL_SET_ME( + ANNOTATE_MOBILE_ELEMENTS.out.vcf, + ch_hgnc_ids + ) + ch_versions = ch_versions.mix( GENERATE_CLINICAL_SET_ME.out.versions ) + + ANN_CSQ_PLI_ME( + GENERATE_CLINICAL_SET_ME.out.vcf, + ch_variant_consequences + ) + ch_versions = ch_versions.mix( ANN_CSQ_PLI_ME.out.versions ) + } // From 6d0f929c2bffb858112364a90e845e00db3aee91 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 12 Apr 2024 14:07:45 +0200 Subject: [PATCH 06/80] remove variant consequences --- subworkflows/local/annotate_mobile_elements.nf | 1 - workflows/raredisease.nf | 3 +-- 2 files changed, 1 insertion(+), 3 deletions(-) diff --git a/subworkflows/local/annotate_mobile_elements.nf b/subworkflows/local/annotate_mobile_elements.nf index cb6087a7..cfd66de9 100644 --- a/subworkflows/local/annotate_mobile_elements.nf +++ b/subworkflows/local/annotate_mobile_elements.nf @@ -16,7 +16,6 @@ workflow ANNOTATE_MOBILE_ELEMENTS { ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] ch_vep_cache // channel: [mandatory] [ path(cache) ] - ch_variant_consequences // channel: [mandatory] [ path(consequences) ] val_vep_genome // string: [mandatory] GRCh37 or GRCh38 val_vep_cache_version // string: [mandatory] default: 107 ch_vep_extra_files // channel: [mandatory] [ path(files) ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 84f68eb6..dc7fde2b 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -669,7 +669,6 @@ workflow RAREDISEASE { ch_genome_fasta, ch_genome_dictionary, ch_vep_cache, - ch_variant_consequences_sv, params.genome, params.vep_cache_version, ch_vep_extra_files @@ -684,7 +683,7 @@ workflow RAREDISEASE { ANN_CSQ_PLI_ME( GENERATE_CLINICAL_SET_ME.out.vcf, - ch_variant_consequences + ch_variant_consequences_sv ) ch_versions = ch_versions.mix( ANN_CSQ_PLI_ME.out.versions ) From d0b512be4b6c542fa8537ea508e7f931e4dab491 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 16 Apr 2024 13:50:37 +0200 Subject: [PATCH 07/80] update test config --- conf/test.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/test.config b/conf/test.config index adc70962..5301f96b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -60,6 +60,7 @@ params { variant_consequences_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" variant_consequences_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" + vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" vep_cache_version = 107 vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" } From 6848a01d739aea984900a2704d5604281263e3c0 Mon Sep 17 00:00:00 2001 From: jemten Date: Fri, 19 Apr 2024 10:04:37 +0200 Subject: [PATCH 08/80] fixing typo --- subworkflows/local/call_snv.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 3e76ab91..72c91ecf 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -74,7 +74,7 @@ workflow CALL_SNV { ch_sentieon_vcf = CALL_SNV_SENTIEON.out.vcf ch_sentieon_tbi = CALL_SNV_SENTIEON.out.tabix ch_sentieon_gvcf = CALL_SNV_SENTIEON.out.gvcf - ch_sentieon_gtbi = CALL_SNV_SENTIEON.out.gtbi + ch_sentieon_gtbi = CALL_SNV_SENTIEON.out.gvcf_tbi ch_versions = ch_versions.mix(CALL_SNV_SENTIEON.out.versions) } From a7e9f95073f0d0762024f6701bda4dfdac264c9b Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 29 Apr 2024 10:43:25 +0200 Subject: [PATCH 09/80] update repeat expansion files --- conf/modules/call_repeat_expansions.config | 21 +++------------------ docs/output.md | 5 ++--- 2 files changed, 5 insertions(+), 21 deletions(-) diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 743b2a31..085d4a41 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -43,27 +43,12 @@ process { } withName: '.*CALL_REPEAT_EXPANSIONS:BCFTOOLS_REHEADER_EXP' { - ext.args = "--temp-prefix ." - ext.prefix = { "${meta.id}_reheader" } - } - - withName: '.*CALL_REPEAT_EXPANSIONS:RENAMESAMPLE_EXP' { + ext.args = "--temp-prefix . -O v" + ext.prefix = { "${meta.id}_repeat_expansion" } publishDir = [ path: { "${params.outdir}/repeat_expansions" }, mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : - filename.contains('_renam.vcf.gz') ? "${meta.id}_repeat_expansion.vcf.gz" : - filename }, - ] - } - - withName: '.*CALL_REPEAT_EXPANSIONS:TABIX_EXP_RENAME' { - publishDir = [ - path: { "${params.outdir}/repeat_expansions" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : - filename.contains('_renam.vcf.gz.tbi') ? "${meta.id}_repeat_expansion.vcf.gz.tbi" : - filename }, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, ] } diff --git a/docs/output.md b/docs/output.md index a0053784..3e44849a 100644 --- a/docs/output.md +++ b/docs/output.md @@ -319,14 +319,13 @@ The pipeline performs variant calling using [Sentieon DNAscope](https://support. #### Expansion Hunter -[Expansion Hunter](https://github.com/Illumina/ExpansionHunter) aims to estimate sizes of repeat sequences by performing a targeted search through alignments that span, flank, and are fully contained in each repeat. +[Expansion Hunter](https://github.com/Illumina/ExpansionHunter) aims to estimate sizes of repeat sequences by performing a targeted search through alignments that span, flank, and are fully contained in each repeat. The files generated are ready to be used with [REViewer](https://github.com/Illumina/REViewer).
Output files - `repeat_expansions/` - - `_repeat_expansion.vcf.gz`: file containing variant calls. - - `_repeat_expansion.vcf.gz.tbi`: index of the file containing variant calls. + - `_repeat_expansion.vcf`: file containing variant calls. - `_exphunter_sorted.bam`: A BAMlet containing alignments of reads that overlap or located in close proximity to each variant identified by ExpansionHunter - `_exphunter_sorted.bam.bai`: Index of the BAMlet file From 8e028140426d218636209eb0843fb2b04f23d249 Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 29 Apr 2024 11:48:33 +0200 Subject: [PATCH 10/80] adding explicit temps --- conf/modules/align_bwa_bwamem2.config | 2 ++ conf/modules/prepare_references.config | 7 ++++++- conf/modules/rank_variants.config | 22 ++++++++++++++-------- conf/modules/raredisease.config | 2 +- 4 files changed, 23 insertions(+), 10 deletions(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index f0725de9..30d14cf4 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -19,12 +19,14 @@ process { withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM2_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwamem2") } } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwa") } } diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 04416bcd..5d24cefa 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -115,7 +115,12 @@ process { } withName: '.*PREPARE_REFERENCES:GATK_PREPROCESS_WGS' { - ext.args = { "--padding 0 --interval-merging-rule OVERLAPPING_ONLY --exclude-intervals ${params.mito_name}" } + ext.args = { [ + '--padding 0', + '--interval-merging-rule OVERLAPPING_ONLY', + "--exclude-intervals ${params.mito_name}", + "--temp-dir ./" + ].join(' ') } ext.when = { params.analysis_type.equals("wgs") && !params.readcount_intervals } } diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index 3d8f1804..66fafd0e 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -21,22 +21,24 @@ process { ext.prefix = { "${meta.id}_sv_genmod_annotate_${meta.set}" } ext.args = { [ '--annotate_regions', - params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38', + '--temp_dir ./' ].join(' ') } } withName: '.*RANK_VARIANTS_SV:GENMOD_MODELS' { ext.prefix = { "${meta.id}_sv_genmod_models_${meta.set}" } - ext.args = " --whole_gene " + ext.args = "--whole_gene --temp_dir ./" } withName: '.*RANK_VARIANTS_SV:GENMOD_SCORE' { ext.prefix = { "${meta.id}_sv_genmod_score_${meta.set}" } - ext.args = " --rank_results " + ext.args = "--rank_results" } withName: '.*RANK_VARIANTS_SV:GENMOD_COMPOUND' { ext.prefix = { "${meta.id}_sv_genmod_compound_${meta.set}" } + ext.args = "--temp_dir ./" } withName: '.*RANK_VARIANTS_SV:BCFTOOLS_SORT' { @@ -73,21 +75,23 @@ process { ext.args = { [ '--annotate_regions', params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + '--temp_dir ./' ].join(' ') } } withName: '.*RANK_VARIANTS_SNV:GENMOD_MODELS' { ext.prefix = { "${meta.id}_snv_genmod_models_${meta.set}" } - ext.args = " --whole_gene " + ext.args = "--whole_gene --temp_dir ./" } withName: '.*RANK_VARIANTS_SNV:GENMOD_SCORE' { ext.prefix = { "${meta.id}_snv_genmod_score_${meta.set}" } - ext.args = " --rank_results " + ext.args = "--rank_results" } withName: '.*RANK_VARIANTS_SNV:GENMOD_COMPOUND' { ext.prefix = { "${meta.id}_snv_genmod_compound_${meta.set}" } + ext.args = "--temp_dir ./" } withName: '.*RANK_VARIANTS_SNV:BCFTOOLS_SORT' { @@ -122,22 +126,24 @@ process { ext.prefix = { "${meta.id}_mt_genmod_annotate_${meta.set}" } ext.args = { [ '--annotate_regions', - params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38', + '--temp_dir ./' ].join(' ') } } withName: '.*RANK_VARIANTS_MT:GENMOD_MODELS' { ext.prefix = { "${meta.id}_mt_genmod_models_${meta.set}" } - ext.args = " --whole_gene " + ext.args = "--whole_gene --temp_dir ./" } withName: '.*RANK_VARIANTS_MT:GENMOD_SCORE' { ext.prefix = { "${meta.id}_mt_genmod_score_${meta.set}" } - ext.args = " --rank_results " + ext.args = "--rank_results" } withName: '.*RANK_VARIANTS_MT:GENMOD_COMPOUND' { ext.prefix = { "${meta.id}_mt_genmod_compound_${meta.set}" } + ext.args = "--temp_dir ./" } withName: '.*RANK_VARIANTS_MT:BCFTOOLS_SORT' { diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index a4f1bbac..12ff0f37 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -33,7 +33,7 @@ process { process { withName: '.*RAREDISEASE:FASTQC' { - ext.args = '--quiet' + ext.args = '--quiet --dir ./' publishDir = [ path: { "${params.outdir}/fastqc/${meta.id}" }, saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, From b2ee216b1f4622ef272cb581ebdcb3dd051f922f Mon Sep 17 00:00:00 2001 From: jemten Date: Mon, 29 Apr 2024 11:56:08 +0200 Subject: [PATCH 11/80] adds trailing comma --- conf/modules/rank_variants.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/rank_variants.config b/conf/modules/rank_variants.config index 66fafd0e..cd5ab7c9 100644 --- a/conf/modules/rank_variants.config +++ b/conf/modules/rank_variants.config @@ -74,7 +74,7 @@ process { ext.prefix = { "${meta.id}_snv_genmod_annotate_${meta.set}" } ext.args = { [ '--annotate_regions', - params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38' + params.genome.equals('GRCh37') ? '--genome-build 37' : '--genome-build 38', '--temp_dir ./' ].join(' ') } } From 77f486e9f5c24d40d3667973f8dc31d1986b8149 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Apr 2024 13:29:34 +0200 Subject: [PATCH 12/80] remove lane from meta --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- subworkflows/local/alignment/align_sentieon.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 10537898..ab5b8d9b 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -44,7 +44,7 @@ workflow ALIGN_BWA_BWAMEM2 { ch_align .map{ meta, bam -> new_id = meta.sample - new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] + new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] - meta.subMap('lane') [groupKey(new_meta, new_meta.num_lanes), bam] } .groupTuple() diff --git a/subworkflows/local/alignment/align_sentieon.nf b/subworkflows/local/alignment/align_sentieon.nf index 366f6cd9..792d9c34 100644 --- a/subworkflows/local/alignment/align_sentieon.nf +++ b/subworkflows/local/alignment/align_sentieon.nf @@ -23,7 +23,7 @@ workflow ALIGN_SENTIEON { .bam_and_bai .map{ meta, bam, bai -> new_id = meta.sample - new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] + new_meta = meta + [id:new_id, read_group:"\'@RG\\tID:" + new_id + "\\tPL:" + val_platform + "\\tSM:" + new_id + "\'"] - meta.subMap('lane') [groupKey(new_meta, new_meta.num_lanes), bam, bai] } .groupTuple() From 62e8e97a68112be6566b2f661933ff647b4fdbd9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Apr 2024 19:15:49 +0200 Subject: [PATCH 13/80] move hmtnote to the top in mt annotation wf --- conf/modules/annotate_mt_snvs.config | 11 ++--- subworkflows/local/annotate_mt_snvs.nf | 57 +++++++++++++++----------- 2 files changed, 40 insertions(+), 28 deletions(-) diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index 8675aff7..ea261a30 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -16,6 +16,11 @@ // process { + withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { + ext.prefix = { "${meta.prefix}" } + ext.args = '--offline' + } + withName: '.*ANNOTATE_MT_SNVS:ENSEMBLVEP_MT' { ext.args = { [ '--dir_plugins vep_cache/Plugins', @@ -38,7 +43,7 @@ process { ext.prefix = { "${meta.prefix}" } } - withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_HMTNOTE' { + withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_VCFANNO_MT' { ext.prefix = { "${meta.prefix}" } publishDir = [ path: { "${params.outdir}/annotate_snv/mitochondria" }, @@ -48,10 +53,6 @@ process { ] } - withName: '.*ANNOTATE_MT_SNVS:HMTNOTE_ANNOTATE' { - ext.prefix = { "${meta.prefix}_uncompressed" } - ext.args = '--offline' - } withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { ext.prefix = { "${meta.prefix}_haplogrep" } diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index 917893b4..f6dbbe5d 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -3,12 +3,13 @@ // include { REPLACE_SPACES_IN_VCFINFO } from '../../modules/local/replace_spaces_in_vcfinfo' -include { TABIX_TABIX as TABIX_TABIX_MT } from '../../modules/nf-core/tabix/tabix/main' +include { TABIX_TABIX as TABIX_TABIX_VEP_MT } from '../../modules/nf-core/tabix/tabix/main' +include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE_MT } from '../../modules/nf-core/tabix/bgziptabix/main' include { ENSEMBLVEP_VEP as ENSEMBLVEP_MT } from '../../modules/nf-core/ensemblvep/vep/main' include { HAPLOGREP2_CLASSIFY as HAPLOGREP2_CLASSIFY_MT } from '../../modules/nf-core/haplogrep2/classify/main' include { VCFANNO as VCFANNO_MT } from '../../modules/nf-core/vcfanno/main' include { ANNOTATE_CADD } from './annotation/annotate_cadd' -include { TABIX_BGZIPTABIX as ZIP_TABIX_HMTNOTE } from '../../modules/nf-core/tabix/bgziptabix/main' +include { TABIX_BGZIPTABIX as ZIP_TABIX_VCFANNO_MT } from '../../modules/nf-core/tabix/bgziptabix/main' include { HMTNOTE_ANNOTATE } from '../../modules/nf-core/hmtnote/annotate/main' workflow ANNOTATE_MT_SNVS { @@ -27,29 +28,45 @@ workflow ANNOTATE_MT_SNVS { ch_vep_extra_files // channel: [mandatory] [ path(files) ] main: - ch_cadd_vcf = Channel.empty() ch_versions = Channel.empty() + // add prefix to meta + ch_mt_vcf + .map { it -> + return [it[0]+ [prefix: it[1].simpleName + "_hmtnote"], it[1]] + } + .set { ch_hmtnote_in } + + // HMTNOTE ANNOTATE + HMTNOTE_ANNOTATE(ch_hmtnote_in) + REPLACE_SPACES_IN_VCFINFO(HMTNOTE_ANNOTATE.out.vcf) + ZIP_TABIX_HMTNOTE_MT(REPLACE_SPACES_IN_VCFINFO.out.vcf) + + ch_hmtnote_vcf = ZIP_TABIX_HMTNOTE_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf]} + ch_hmtnote_tbi = ZIP_TABIX_HMTNOTE_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi]} + // Annotating with CADD if (params.cadd_resources != null) { ANNOTATE_CADD ( - ch_mt_vcf, - ch_mt_tbi, + ch_hmtnote_vcf, + ch_hmtnote_tbi, ch_cadd_header, ch_cadd_resources ) ch_cadd_vcf = ANNOTATE_CADD.out.vcf ch_versions = ch_versions.mix(ANNOTATE_CADD.out.versions) + } else { + ch_cadd_vcf = Channel.empty() } // Pick input for vep - ch_mt_vcf + ch_hmtnote_vcf .join(ch_cadd_vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] merged: it[2].equals(null) - return [it[0]+ [prefix: it[1].simpleName + "_vep"], it[1]] + return [it[0]+ [prefix: it[0].prefix + "_vep"], it[1]] cadd: !(it[2].equals(null)) - return [it[0] + [prefix: it[1].simpleName + "_cadd_vep"], it[2]] + return [it[0] + [prefix: it[0].prefix + "_cadd_vep"], it[2]] } .set { ch_for_mix } @@ -58,7 +75,6 @@ workflow ANNOTATE_MT_SNVS { .map { meta, vcf -> return [meta, vcf, []] } .set { ch_vep_in } - // Annotating with ensembl Vep ENSEMBLVEP_MT( ch_vep_in, @@ -71,34 +87,29 @@ workflow ANNOTATE_MT_SNVS { ) // Running vcfanno - TABIX_TABIX_MT(ENSEMBLVEP_MT.out.vcf) + TABIX_TABIX_VEP_MT(ENSEMBLVEP_MT.out.vcf) ENSEMBLVEP_MT.out.vcf - .join(TABIX_TABIX_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) + .join(TABIX_TABIX_VEP_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) .map { meta, vcf, tbi -> return [meta + [prefix: meta.prefix + "_vcfanno"], vcf, tbi, []]} .set { ch_in_vcfanno } - VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources).vcf - .map { meta, vcf -> return [meta + [prefix: meta.prefix + "_hmtnote"], vcf]} - .set {ch_hmtnote_in} - - // HMTNOTE ANNOTATE - HMTNOTE_ANNOTATE(ch_hmtnote_in) - REPLACE_SPACES_IN_VCFINFO(HMTNOTE_ANNOTATE.out.vcf) - ZIP_TABIX_HMTNOTE(REPLACE_SPACES_IN_VCFINFO.out.vcf) + VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources) + ZIP_TABIX_VCFANNO_MT(VCFANNO_MT.out.vcf) // Prepare output - ch_vcf_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf] } - ch_tbi_out = ZIP_TABIX_HMTNOTE.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi] } + ch_vcf_out = ZIP_TABIX_VCFANNO_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf] } + ch_tbi_out = ZIP_TABIX_VCFANNO_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi] } // Running haplogrep2 HAPLOGREP2_CLASSIFY_MT(ch_haplogrep_in, "vcf.gz") ch_versions = ch_versions.mix(ENSEMBLVEP_MT.out.versions) - ch_versions = ch_versions.mix(TABIX_TABIX_MT.out.versions) + ch_versions = ch_versions.mix(TABIX_TABIX_VEP_MT.out.versions) ch_versions = ch_versions.mix(VCFANNO_MT.out.versions) ch_versions = ch_versions.mix(HMTNOTE_ANNOTATE.out.versions) ch_versions = ch_versions.mix(HAPLOGREP2_CLASSIFY_MT.out.versions) - ch_versions = ch_versions.mix(ZIP_TABIX_HMTNOTE.out.versions) + ch_versions = ch_versions.mix(ZIP_TABIX_VCFANNO_MT.out.versions) + ch_versions = ch_versions.mix(ZIP_TABIX_HMTNOTE_MT.out.versions) ch_versions = ch_versions.mix(REPLACE_SPACES_IN_VCFINFO.out.versions) emit: From c186c5b81e7f46e9b6f8b934dbea0e20c038d6d9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Apr 2024 19:22:22 +0200 Subject: [PATCH 14/80] remove blank line --- conf/modules/annotate_mt_snvs.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index ea261a30..b740176d 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -53,7 +53,6 @@ process { ] } - withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { ext.prefix = { "${meta.prefix}_haplogrep" } publishDir = [ From 9bfeae04d9bbe8029f802f374928a88a5aa9cb4f Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Apr 2024 23:10:47 +0200 Subject: [PATCH 15/80] update pedigree file --- conf/modules/prepare_references.config | 2 +- conf/modules/raredisease.config | 14 +++++++ lib/CustomFunctions.groovy | 16 -------- modules/local/create_pedigree_file.nf | 53 ++++++++++++++++++++++++++ workflows/raredisease.nf | 6 ++- 5 files changed, 73 insertions(+), 18 deletions(-) create mode 100644 modules/local/create_pedigree_file.nf diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 5d24cefa..103a1ad7 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -119,7 +119,7 @@ process { '--padding 0', '--interval-merging-rule OVERLAPPING_ONLY', "--exclude-intervals ${params.mito_name}", - "--temp-dir ./" + "--tmp-dir ./" ].join(' ') } ext.when = { params.analysis_type.equals("wgs") && !params.readcount_intervals } } diff --git a/conf/modules/raredisease.config b/conf/modules/raredisease.config index 12ff0f37..12d77a06 100644 --- a/conf/modules/raredisease.config +++ b/conf/modules/raredisease.config @@ -73,6 +73,20 @@ process { } } +// +// Generate Pedigree File +// + +process { + + withName: '.*RAREDISEASE:CREATE_PEDIGREE_FILE' { + publishDir = [ + path: { "${params.outdir}/pedigree" }, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } +} + // // Multiqc options // diff --git a/lib/CustomFunctions.groovy b/lib/CustomFunctions.groovy index 0ff3eadd..1a8d0227 100644 --- a/lib/CustomFunctions.groovy +++ b/lib/CustomFunctions.groovy @@ -2,22 +2,6 @@ import nextflow.Nextflow class CustomFunctions { - // Function to generate a pedigree file - public static File makePed(samples, outdir) { - - def case_name = samples[0].case_id - def outfile = new File(outdir +"/pipeline_info/${case_name}" + '.ped') - outfile.text = ['#family_id', 'sample_id', 'father', 'mother', 'sex', 'phenotype'].join('\t') - def samples_list = [] - for(int i = 0; i${case_name}.ped + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + create_pedigree_file: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ + + stub: + def case_name = samples[0].case_id + """ + touch ${case_name}.ped + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + create_pedigree_file: v1.0 + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index dc7fde2b..68b1cc1f 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -113,6 +113,7 @@ include { SMNCOPYNUMBERCALLER } from '../modules/nf-core/smncopynumbercaller/mai include { RENAME_ALIGN_FILES as RENAME_BAM_FOR_SMNCALLER } from '../modules/local/rename_align_files' include { RENAME_ALIGN_FILES as RENAME_BAI_FOR_SMNCALLER } from '../modules/local/rename_align_files' include { CREATE_HGNCIDS_FILE } from '../modules/local/create_hgncids_file' +include { CREATE_PEDIGREE_FILE } from '../modules/local/create_pedigree_file' // // SUBWORKFLOWS @@ -163,7 +164,6 @@ workflow RAREDISEASE { ch_multiqc_files = Channel.empty() ch_samples = ch_samplesheet.map { meta, fastqs -> meta} - ch_pedfile = ch_samples.toList().map { file(CustomFunctions.makePed(it, params.outdir)) } ch_case_info = ch_samples.toList().map { CustomFunctions.createCaseChannel(it) } // Initialize file channels for PREPARE_REFERENCES subworkflow @@ -301,6 +301,10 @@ workflow RAREDISEASE { ch_svcaller_priority = Channel.value(["tiddit", "manta", "gcnvcaller", "cnvnator"]) } + + // Generate pedigree file + ch_pedfile = CREATE_PEDIGREE_FILE(ch_samples.toList()) + // Read and store paths in the vep_plugin_files file if (params.vep_plugin_files) { ch_vep_extra_files_unsplit.splitCsv ( header:true ) From e9b15db0d0bbee44100ff0e7d6906dd0cb9be55d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Apr 2024 23:15:44 +0200 Subject: [PATCH 16/80] fix error --- workflows/raredisease.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 68b1cc1f..1bd76e08 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -303,7 +303,8 @@ workflow RAREDISEASE { // Generate pedigree file - ch_pedfile = CREATE_PEDIGREE_FILE(ch_samples.toList()) + ch_pedfile = CREATE_PEDIGREE_FILE(ch_samples.toList()).out.ped + ch_versions = ch_versions.mix(CREATE_PEDIGREE_FILE.out.versions()) // Read and store paths in the vep_plugin_files file if (params.vep_plugin_files) { From eaa87b6c016f184796a04e823ea90fae9fdce2b7 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Apr 2024 23:26:05 +0200 Subject: [PATCH 17/80] fix versions --- workflows/raredisease.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 1bd76e08..00805e43 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -303,8 +303,8 @@ workflow RAREDISEASE { // Generate pedigree file - ch_pedfile = CREATE_PEDIGREE_FILE(ch_samples.toList()).out.ped - ch_versions = ch_versions.mix(CREATE_PEDIGREE_FILE.out.versions()) + ch_pedfile = CREATE_PEDIGREE_FILE(ch_samples.toList()).ped + ch_versions = ch_versions.mix(CREATE_PEDIGREE_FILE.out.versions) // Read and store paths in the vep_plugin_files file if (params.vep_plugin_files) { From 2b3ae4f90ab6bdc1b78c8d15f932eb0851558c86 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 29 Apr 2024 23:43:52 +0200 Subject: [PATCH 18/80] update output docs --- docs/output.md | 9 +++++---- 1 file changed, 5 insertions(+), 4 deletions(-) diff --git a/docs/output.md b/docs/output.md index 3e44849a..a872c036 100644 --- a/docs/output.md +++ b/docs/output.md @@ -66,7 +66,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [CADD](#cadd-1) - [Hmtnote](#hmtnote) - [VEP](#vep-2) - - [Rank variants and filtering](#rank-variants-and-filtering) + - [Filtering and ranking](#filtering-and-ranking) + - [Filter\_vep](#filter_vep) - [GENMOD](#genmod) - [Mobile element analysis](#mobile-element-analysis) - [Calling mobile elements](#calling-mobile-elements) @@ -478,7 +479,7 @@ The pipeline for mitochondrial variant discovery, using Mutect2, uses a high sen Output files - `annotate_snv/mitochondria` - - `_mitochondria_haplogrep.txt`: file containing haplogroup information. + - `*haplogrep.txt`: file containing haplogroup information.
@@ -504,8 +505,8 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files Output files - `annotate_snv/mitochondria` - - `_mitochondria_vep_vcfanno_hmtnote.vcf.gz`: file containing mitochondrial annotations. - - `_mitochondria_vep_vcfanno_hmtnote.vcf.gz.tbi`: index of the file containing mitochondrial annotations. + - `_mitochondria_hmtnote_vep_vcfanno.vcf.gz`: file containing mitochondrial annotations. + - `_mitochondria_hmtnote_vep_vcfanno.vcf.gz.tbi`: index of the file containing mitochondrial annotations. From 4fa280df0bdcec3a38a59ce1c01c7d35da677ee6 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 30 Apr 2024 06:53:58 +0200 Subject: [PATCH 19/80] fix reheader args --- conf/modules/call_repeat_expansions.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 085d4a41..d76ae186 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -43,7 +43,7 @@ process { } withName: '.*CALL_REPEAT_EXPANSIONS:BCFTOOLS_REHEADER_EXP' { - ext.args = "--temp-prefix . -O v" + ext.args = "--temp-prefix ." ext.prefix = { "${meta.id}_repeat_expansion" } publishDir = [ path: { "${params.outdir}/repeat_expansions" }, From 30128fc03a631571b16d935e88eacd02b98231d5 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 30 Apr 2024 13:30:05 +0200 Subject: [PATCH 20/80] ignore error from bwa-mem2 and if output is missing, run bwa --- conf/modules/align_bwa_bwamem2.config | 1 + subworkflows/local/alignment/align_bwa_bwamem2.nf | 10 +++++----- 2 files changed, 6 insertions(+), 5 deletions(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index e91360fc..d82fb5d6 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -23,6 +23,7 @@ process { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwamem2") } + label = 'error_ignore' } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 10537898..6d5847a7 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -24,14 +24,14 @@ workflow ALIGN_BWA_BWAMEM2 { ch_versions = Channel.empty() // Map, sort, and index - if (params.aligner.equals("bwa")) { - BWA_MEM ( ch_reads_input, ch_bwa_index, true ) - ch_align = BWA_MEM.out.bam - ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) - } else { + if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) + else if (params.aligner.equals("bwa") || !BWAMEM2_MEM.out.bam.exists()) { + BWA_MEM ( ch_reads_input, ch_bwa_index, true ) + ch_align = BWA_MEM.out.bam + ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) } SAMTOOLS_INDEX_ALIGN ( ch_align ) From 5accab2506ea70661860f038631c0dedfc9f781f Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 30 Apr 2024 13:30:41 +0200 Subject: [PATCH 21/80] change logical gate --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 6d5847a7..650c5fc6 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -28,7 +28,7 @@ workflow ALIGN_BWA_BWAMEM2 { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - else if (params.aligner.equals("bwa") || !BWAMEM2_MEM.out.bam.exists()) { + else if (params.aligner.equals("bwa") || (params.aligner.equals("bwamem2") && !BWAMEM2_MEM.out.bam.exists())) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_align = BWA_MEM.out.bam ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) From afeefc1a5c221ff174571d4878db92aea3e97e56 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 30 Apr 2024 13:36:47 +0200 Subject: [PATCH 22/80] typo --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 650c5fc6..e26d4bde 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -28,6 +28,7 @@ workflow ALIGN_BWA_BWAMEM2 { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) + } else if (params.aligner.equals("bwa") || (params.aligner.equals("bwamem2") && !BWAMEM2_MEM.out.bam.exists())) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_align = BWA_MEM.out.bam From 7eae508bb1e7b62ce408a671843114ff4c32b995 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 30 Apr 2024 13:48:00 +0200 Subject: [PATCH 23/80] move vcfanno to the top of anno mt snvs workflow --- conf/modules/annotate_mt_snvs.config | 21 +++++++++---- conf/modules/call_repeat_expansions.config | 3 +- docs/output.md | 6 ++-- subworkflows/local/annotate_mt_snvs.nf | 34 ++++++++++------------ 4 files changed, 35 insertions(+), 29 deletions(-) diff --git a/conf/modules/annotate_mt_snvs.config b/conf/modules/annotate_mt_snvs.config index b740176d..0d0026cf 100644 --- a/conf/modules/annotate_mt_snvs.config +++ b/conf/modules/annotate_mt_snvs.config @@ -37,6 +37,21 @@ process { '--uniprot' ].join(' ') } ext.prefix = { "${meta.prefix}" } + publishDir = [ + path: { "${params.outdir}/annotate_snv/mitochondria" }, + mode: params.publish_dir_mode, + pattern: "*{vcf.gz,vcf.gz.tbi}", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: '.*ANNOTATE_MT_SNVS:TABIX_TABIX_VEP_MT' { + publishDir = [ + path: { "${params.outdir}/annotate_snv/mitochondria" }, + mode: params.publish_dir_mode, + pattern: "*{vcf.gz,vcf.gz.tbi}", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] } withName: '.*ANNOTATE_MT_SNVS:VCFANNO_MT' { @@ -45,12 +60,6 @@ process { withName: '.*ANNOTATE_MT_SNVS:ZIP_TABIX_VCFANNO_MT' { ext.prefix = { "${meta.prefix}" } - publishDir = [ - path: { "${params.outdir}/annotate_snv/mitochondria" }, - mode: params.publish_dir_mode, - pattern: "*{vcf.gz,vcf.gz.tbi}", - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] } withName: '.*ANNOTATE_MT_SNVS:HAPLOGREP2_CLASSIFY_MT' { diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index d76ae186..002ddffb 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -43,7 +43,8 @@ process { } withName: '.*CALL_REPEAT_EXPANSIONS:BCFTOOLS_REHEADER_EXP' { - ext.args = "--temp-prefix ." + ext.args = "--temp-prefix ." + ext.args2 = "-O v" ext.prefix = { "${meta.id}_repeat_expansion" } publishDir = [ path: { "${params.outdir}/repeat_expansions" }, diff --git a/docs/output.md b/docs/output.md index a872c036..3241f5d4 100644 --- a/docs/output.md +++ b/docs/output.md @@ -67,7 +67,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Hmtnote](#hmtnote) - [VEP](#vep-2) - [Filtering and ranking](#filtering-and-ranking) - - [Filter\_vep](#filter_vep) + - [Filter_vep](#filter_vep) - [GENMOD](#genmod) - [Mobile element analysis](#mobile-element-analysis) - [Calling mobile elements](#calling-mobile-elements) @@ -505,8 +505,8 @@ We recommend using vcfanno to annotate SNVs with precomputed CADD scores (files Output files - `annotate_snv/mitochondria` - - `_mitochondria_hmtnote_vep_vcfanno.vcf.gz`: file containing mitochondrial annotations. - - `_mitochondria_hmtnote_vep_vcfanno.vcf.gz.tbi`: index of the file containing mitochondrial annotations. + - `_mitochondria_hmtnote_vcfanno_.vcf.gz`: file containing mitochondrial annotations. + - `_mitochondria_hmtnote_vcfanno_.vcf.gz.tbi`: index of the file containing mitochondrial annotations. diff --git a/subworkflows/local/annotate_mt_snvs.nf b/subworkflows/local/annotate_mt_snvs.nf index f6dbbe5d..02554461 100644 --- a/subworkflows/local/annotate_mt_snvs.nf +++ b/subworkflows/local/annotate_mt_snvs.nf @@ -42,14 +42,22 @@ workflow ANNOTATE_MT_SNVS { REPLACE_SPACES_IN_VCFINFO(HMTNOTE_ANNOTATE.out.vcf) ZIP_TABIX_HMTNOTE_MT(REPLACE_SPACES_IN_VCFINFO.out.vcf) - ch_hmtnote_vcf = ZIP_TABIX_HMTNOTE_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf]} - ch_hmtnote_tbi = ZIP_TABIX_HMTNOTE_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi]} + // Vcfanno + ZIP_TABIX_HMTNOTE_MT.out.gz_tbi + .map { meta, vcf, tbi -> return [meta + [prefix: meta.prefix + "_vcfanno"], vcf, tbi, []]} + .set { ch_in_vcfanno } + + VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources) + ZIP_TABIX_VCFANNO_MT(VCFANNO_MT.out.vcf) + + ch_vcfanno_vcf = ZIP_TABIX_VCFANNO_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf]} + ch_vcfanno_tbi = ZIP_TABIX_VCFANNO_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi]} // Annotating with CADD if (params.cadd_resources != null) { ANNOTATE_CADD ( - ch_hmtnote_vcf, - ch_hmtnote_tbi, + ch_vcfanno_vcf, + ch_vcfanno_tbi, ch_cadd_header, ch_cadd_resources ) @@ -60,7 +68,7 @@ workflow ANNOTATE_MT_SNVS { } // Pick input for vep - ch_hmtnote_vcf + ch_vcfanno_vcf .join(ch_cadd_vcf, remainder: true) // If CADD is not run then the third element in this channel will be `null` .branch { it -> // If CADD is run, then "it" will be [[meta],selvar.vcf,cadd.vcf], else [[meta],selvar.vcf,null] merged: it[2].equals(null) @@ -86,19 +94,7 @@ workflow ANNOTATE_MT_SNVS { ch_vep_extra_files ) - // Running vcfanno TABIX_TABIX_VEP_MT(ENSEMBLVEP_MT.out.vcf) - ENSEMBLVEP_MT.out.vcf - .join(TABIX_TABIX_VEP_MT.out.tbi, failOnMismatch:true, failOnDuplicate:true) - .map { meta, vcf, tbi -> return [meta + [prefix: meta.prefix + "_vcfanno"], vcf, tbi, []]} - .set { ch_in_vcfanno } - - VCFANNO_MT(ch_in_vcfanno, ch_vcfanno_toml, [], ch_vcfanno_resources) - ZIP_TABIX_VCFANNO_MT(VCFANNO_MT.out.vcf) - - // Prepare output - ch_vcf_out = ZIP_TABIX_VCFANNO_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, vcf] } - ch_tbi_out = ZIP_TABIX_VCFANNO_MT.out.gz_tbi.map{meta, vcf, tbi -> return [meta, tbi] } // Running haplogrep2 HAPLOGREP2_CLASSIFY_MT(ch_haplogrep_in, "vcf.gz") @@ -114,8 +110,8 @@ workflow ANNOTATE_MT_SNVS { emit: haplog = HAPLOGREP2_CLASSIFY_MT.out.txt // channel: [ val(meta), path(txt) ] - vcf_ann = ch_vcf_out // channel: [ val(meta), path(vcf) ] - tbi = ch_tbi_out // channel: [ val(meta), path(tbi) ] + vcf_ann = ENSEMBLVEP_MT.out.vcf // channel: [ val(meta), path(vcf) ] + tbi = TABIX_TABIX_VEP_MT.out.tbi // channel: [ val(meta), path(tbi) ] report = ENSEMBLVEP_MT.out.report // channel: [ path(html) ] versions = ch_versions // channel: [ path(versions.yml) ] } From 2d2efd2382de6698786686b3572897b1a3e322fd Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 30 Apr 2024 13:49:25 +0200 Subject: [PATCH 24/80] change syntax --- conf/modules/align_bwa_bwamem2.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index d82fb5d6..cf5e79f8 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -23,7 +23,7 @@ process { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwamem2") } - label = 'error_ignore' + errorStrategy = 'ignore' } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { From 52b7caa8411216e3f04e9633a2871851cea504c0 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 30 Apr 2024 13:53:37 +0200 Subject: [PATCH 25/80] change logical gate --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index e26d4bde..6ee8dfc9 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -29,7 +29,7 @@ workflow ALIGN_BWA_BWAMEM2 { ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) } - else if (params.aligner.equals("bwa") || (params.aligner.equals("bwamem2") && !BWAMEM2_MEM.out.bam.exists())) { + if (params.aligner.equals("bwa") || (params.aligner.equals("bwamem2") && !BWAMEM2_MEM.out.bam.exists())) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_align = BWA_MEM.out.bam ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) From 2c0bdb028434045076490960bc51c7ee53ec9dd1 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 30 Apr 2024 14:24:45 +0200 Subject: [PATCH 26/80] change logical gate --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 6ee8dfc9..acf684e0 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -22,14 +22,15 @@ workflow ALIGN_BWA_BWAMEM2 { main: ch_versions = Channel.empty() - + count = 0 // Map, sort, and index if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) + count = BWAMEM2_MEM.out.bam.ifEmpty(1) } - if (params.aligner.equals("bwa") || (params.aligner.equals("bwamem2") && !BWAMEM2_MEM.out.bam.exists())) { + if (params.aligner.equals("bwa") || count == 1) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_align = BWA_MEM.out.bam ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) From ecd62865665c2026f85587607f9661726017ba02 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Thu, 2 May 2024 11:38:53 +0200 Subject: [PATCH 27/80] try a retry with more memory --- conf/modules/align_bwa_bwamem2.config | 2 +- subworkflows/local/alignment/align_bwa_bwamem2.nf | 14 ++++++-------- 2 files changed, 7 insertions(+), 9 deletions(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index cf5e79f8..ff53af13 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -23,7 +23,7 @@ process { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwamem2") } - errorStrategy = 'ignore' + errorStrategy = { task.exitStatus in (1 + (130..145) + 104) ? 'retry' : 'finish' } } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index acf684e0..10537898 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -22,18 +22,16 @@ workflow ALIGN_BWA_BWAMEM2 { main: ch_versions = Channel.empty() - count = 0 + // Map, sort, and index - if (params.aligner.equals("bwamem2")) { - BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) - ch_align = BWAMEM2_MEM.out.bam - ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - count = BWAMEM2_MEM.out.bam.ifEmpty(1) - } - if (params.aligner.equals("bwa") || count == 1) { + if (params.aligner.equals("bwa")) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_align = BWA_MEM.out.bam ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) + } else { + BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) + ch_align = BWAMEM2_MEM.out.bam + ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) } SAMTOOLS_INDEX_ALIGN ( ch_align ) From eee78184da59049ce512d1308aebf28a0ba8d2d3 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Thu, 2 May 2024 13:50:05 +0200 Subject: [PATCH 28/80] try a retry with more memory --- conf/modules/align_bwa_bwamem2.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index ff53af13..04739e9a 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -23,7 +23,7 @@ process { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwamem2") } - errorStrategy = { task.exitStatus in (1 + (130..145) + 104) ? 'retry' : 'finish' } + errorStrategy = { task.exitStatus in (1, (130..145), 104) ? 'retry' : 'finish' } } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { From 85fc2ea7fbcac4848899852ff81b3b9661b6dc28 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Thu, 2 May 2024 14:22:21 +0200 Subject: [PATCH 29/80] try a retry with more memory --- conf/modules/align_bwa_bwamem2.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index 04739e9a..30d961e7 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -23,7 +23,7 @@ process { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwamem2") } - errorStrategy = { task.exitStatus in (1, (130..145), 104) ? 'retry' : 'finish' } + errorStrategy = { task.exitStatus in [1,104,130,131,132,133,134,135,135,136,137,138,139,140,141,142,143,144,145] ? 'retry' : 'finish' } } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { From f6d99dcb548e8e4679842c4d0c862c57bbc2f4be Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Thu, 2 May 2024 16:32:11 +0200 Subject: [PATCH 30/80] change logic --- conf/modules/align_bwa_bwamem2.config | 2 +- .../local/alignment/align_bwa_bwamem2.nf | 17 +++++++++++------ 2 files changed, 12 insertions(+), 7 deletions(-) diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index 30d961e7..cf5e79f8 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -23,7 +23,7 @@ process { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwamem2") } - errorStrategy = { task.exitStatus in [1,104,130,131,132,133,134,135,135,136,137,138,139,140,141,142,143,144,145] ? 'retry' : 'finish' } + errorStrategy = 'ignore' } withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 10537898..3499551e 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -24,14 +24,19 @@ workflow ALIGN_BWA_BWAMEM2 { ch_versions = Channel.empty() // Map, sort, and index - if (params.aligner.equals("bwa")) { - BWA_MEM ( ch_reads_input, ch_bwa_index, true ) - ch_align = BWA_MEM.out.bam - ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) - } else { + if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) - ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) + + + if (BWA_MEM.out.bam.ifEmpty()) { + BWA_MEM ( ch_reads_input, ch_bwa_index, true ) + ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) + ch_align = BWA_MEM.out.bam + + else { + ch_align = BWAMEM2_MEM.out.bam + } } SAMTOOLS_INDEX_ALIGN ( ch_align ) From 17ed5d4900efc57fa0b0c4ad9d183c15056f1130 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Thu, 2 May 2024 16:44:17 +0200 Subject: [PATCH 31/80] change logic --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 3499551e..62fb99df 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -29,11 +29,11 @@ workflow ALIGN_BWA_BWAMEM2 { ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - if (BWA_MEM.out.bam.ifEmpty()) { + if (BWAMEM2_MEM.out.bam.ifEmpty()) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam - + } else { ch_align = BWAMEM2_MEM.out.bam } From 8adc86f77838dae0ed6580e85f3a87e9791f88aa Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Thu, 2 May 2024 17:00:33 +0200 Subject: [PATCH 32/80] change logic --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 62fb99df..8c7a189a 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -29,7 +29,7 @@ workflow ALIGN_BWA_BWAMEM2 { ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - if (BWAMEM2_MEM.out.bam.ifEmpty()) { + if (BWAMEM2_MEM.out.bam.ifEmpty(False)) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam From ad00cbc9474d2479c3c28f85ceb8a18bf5214847 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 3 May 2024 11:01:05 +0200 Subject: [PATCH 33/80] change if statement --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 14 +++++++++----- 1 file changed, 9 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 8c7a189a..79ca1640 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -22,22 +22,26 @@ workflow ALIGN_BWA_BWAMEM2 { main: ch_versions = Channel.empty() + ch_align = Channel.empty() // Map, sort, and index if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) + ch_align = BWAMEM2_MEM.out.bam - - if (BWAMEM2_MEM.out.bam.ifEmpty(False)) { + if (ch_align == Channel.empty) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam } - else { - ch_align = BWAMEM2_MEM.out.bam - } } + else if (params.aligner.equals("bwamem2"){ + BWA_MEM ( ch_reads_input, ch_bwa_index, true ) + ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) + ch_align = BWA_MEM.out.bam + } + SAMTOOLS_INDEX_ALIGN ( ch_align ) From 7ba937f31556c80f6908270bcd3136829b78277a Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 3 May 2024 11:09:58 +0200 Subject: [PATCH 34/80] change if statement --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 79ca1640..78c4974c 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -30,13 +30,13 @@ workflow ALIGN_BWA_BWAMEM2 { ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) ch_align = BWAMEM2_MEM.out.bam - if (ch_align == Channel.empty) { + if (ch_align == Channel.empty()) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam } } - else if (params.aligner.equals("bwamem2"){ + else if (params.aligner.equals("bwa")){ BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam From b2c0eb1855f99060d252e4cc53fc0796e50a5288 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 3 May 2024 11:37:14 +0200 Subject: [PATCH 35/80] change if statement --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 13 ++++--------- 1 file changed, 4 insertions(+), 9 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 78c4974c..acc1546e 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -22,26 +22,21 @@ workflow ALIGN_BWA_BWAMEM2 { main: ch_versions = Channel.empty() - ch_align = Channel.empty() // Map, sort, and index if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - ch_align = BWAMEM2_MEM.out.bam - if (ch_align == Channel.empty()) { + if (!file.exists(BWAMEM2_MEM.out.bam)) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam } - } - else if (params.aligner.equals("bwa")){ - BWA_MEM ( ch_reads_input, ch_bwa_index, true ) - ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) - ch_align = BWA_MEM.out.bam + else { + ch_align = BWAMEM2_MEM.out.bam } - + } SAMTOOLS_INDEX_ALIGN ( ch_align ) From 98e202a017058d041a501186068d412ea385f088 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 3 May 2024 12:10:25 +0200 Subject: [PATCH 36/80] change if statement --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index acc1546e..0871b063 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -28,7 +28,7 @@ workflow ALIGN_BWA_BWAMEM2 { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - if (!file.exists(BWAMEM2_MEM.out.bam)) { + if (!(BWAMEM2_MEM.out.bam).exists()) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam From e811efd82a5523ff38fe53d4b487bb5292504af1 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 3 May 2024 12:53:41 +0200 Subject: [PATCH 37/80] change if statement --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 0871b063..730a95e4 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -28,7 +28,7 @@ workflow ALIGN_BWA_BWAMEM2 { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - if (!(BWAMEM2_MEM.out.bam).exists()) { + if ((BWAMEM2_MEM.out.bam).isEmpty()) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam From 68f9bc892d7cc46c6f4fa1546891f2ba6b34631a Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 3 May 2024 14:31:29 +0200 Subject: [PATCH 38/80] add tmp channel --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 730a95e4..f7e5cb19 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -22,13 +22,13 @@ workflow ALIGN_BWA_BWAMEM2 { main: ch_versions = Channel.empty() - + ch_align_tmp = Channel.empty() // Map, sort, and index if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - - if ((BWAMEM2_MEM.out.bam).isEmpty()) { + ch_align_tmp = BWAMEM2_MEM.out.bam + if (!ch_align_tmp) { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) ch_align = BWA_MEM.out.bam From 8192e855cdf680d621081ffa70f7168bcf677ba2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Mon, 6 May 2024 15:17:57 +0200 Subject: [PATCH 39/80] Include "other" variants for CADD to analyse multallelic indels --- conf/modules/annotate_cadd.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules/annotate_cadd.config b/conf/modules/annotate_cadd.config index 4ac5b618..404e71e5 100644 --- a/conf/modules/annotate_cadd.config +++ b/conf/modules/annotate_cadd.config @@ -21,7 +21,7 @@ process { } withName: '.*:ANNOTATE_CADD:BCFTOOLS_VIEW' { - ext.args = { "--output-type z --types indels" } + ext.args = { "--output-type z --types indels,other" } ext.prefix = { "${vcf.simpleName}_indels" } } From e6b62b7a9504f4db4bd1af7bc38a7d852790a122 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 6 May 2024 16:09:41 +0200 Subject: [PATCH 40/80] update align --- .../local/alignment/align_bwa_bwamem2.nf | 34 +++++++++++++------ 1 file changed, 24 insertions(+), 10 deletions(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index f7e5cb19..16fff1bc 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -3,6 +3,7 @@ // include { BWA_MEM } from '../../../modules/nf-core/bwa/mem/main' +include { BWA_MEM as BWAMEM_FALLBACK } from '../../../modules/nf-core/bwa/mem/main' include { BWAMEM2_MEM } from '../../../modules/nf-core/bwamem2/mem/main' include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_ALIGN } from '../../../modules/nf-core/samtools/index/main' include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_MARKDUP } from '../../../modules/nf-core/samtools/index/main' @@ -22,20 +23,33 @@ workflow ALIGN_BWA_BWAMEM2 { main: ch_versions = Channel.empty() - ch_align_tmp = Channel.empty() + // Map, sort, and index - if (params.aligner.equals("bwamem2")) { + if (params.aligner.equals("bwa")) { + BWA_MEM ( ch_reads_input, ch_bwa_index, true ) + ch_align = BWA_MEM.out.bam + ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) + } else { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) + ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - ch_align_tmp = BWAMEM2_MEM.out.bam - if (!ch_align_tmp) { - BWA_MEM ( ch_reads_input, ch_bwa_index, true ) - ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) - ch_align = BWA_MEM.out.bam - } - else { - ch_align = BWAMEM2_MEM.out.bam + + if (params.align_fallback.equals(true)) { + ch_reads_input + .join(BWAMEM2_MEM.out.bam, remainder: true) + .branch { it -> + ERROR: it[2].equals(null) + return [it[0], it[1]] // return reads + SUCCESS: !it[2].equals(null) + return [it[0], it[2]] // return bam + } + .set { ch_fallback } + + BWAMEM_FALLBACK ( ch_fallback.ERROR, ch_bwa_index, true ) + ch_align = ch_fallback.SUCCESS.mix(BWAMEM_FALLBACK.out.bam) + ch_versions = ch_versions.mix(BWAMEM_FALLBACK.out.versions.first()) } + } SAMTOOLS_INDEX_ALIGN ( ch_align ) From d15cf7e9009cb19259632d4180263b63f2752052 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Marius=20Bj=C3=B8rnstad?= Date: Mon, 6 May 2024 17:30:45 +0200 Subject: [PATCH 41/80] Describe change for #545 --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index b5243d01..98537916 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,6 +11,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Fixed` +- Include multiallelic indel sites in CADD scoring jobs [#545](https://github.com/nf-core/raredisease/pull/545) + ### Parameters | Old parameter | New parameter | From cad7ce23becf08d7033f5621927f5bc4046a6eac Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 7 May 2024 10:40:32 +0200 Subject: [PATCH 42/80] update param --- nextflow.config | 1 + nextflow_schema.json | 5 +++++ subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- 3 files changed, 7 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 5065d2d9..fb385fa6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -22,6 +22,7 @@ params { // Main options analysis_type = 'wgs' + bwa_as_fallback = false bait_padding = 100 run_rtgvcfeval = false save_mapped_as_cram = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 6e2fbc81..1d1049bd 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -459,6 +459,11 @@ "fa_icon": "fas fa-user-cog", "description": "Options used to steer the direction of the pipeline.", "properties": { + "bwa_as_fallback": { + "type": "boolean", + "description": "Specifies whether or not to use bwa as a fallback aligner in case bwamem2 throws an error.", + "fa_icon": "fas fa-toggle-on" + }, "analysis_type": { "type": "string", "default": "wgs", diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 80bf3822..40e93196 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -34,7 +34,7 @@ workflow ALIGN_BWA_BWAMEM2 { ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - if (params.align_fallback.equals(true)) { + if (params.bwa_as_fallback.equals(true)) { ch_reads_input .join(BWAMEM2_MEM.out.bam, remainder: true) .branch { it -> From 7efe3b876169a97e9c03eb214efe703cdbd01e8a Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 7 May 2024 15:04:19 +0200 Subject: [PATCH 43/80] arrange param --- nextflow_schema.json | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 1d1049bd..d70f69ed 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -459,11 +459,6 @@ "fa_icon": "fas fa-user-cog", "description": "Options used to steer the direction of the pipeline.", "properties": { - "bwa_as_fallback": { - "type": "boolean", - "description": "Specifies whether or not to use bwa as a fallback aligner in case bwamem2 throws an error.", - "fa_icon": "fas fa-toggle-on" - }, "analysis_type": { "type": "string", "default": "wgs", @@ -471,6 +466,11 @@ "fa_icon": "fas fa-align-center", "enum": ["wgs", "wes", "mito"] }, + "bwa_as_fallback": { + "type": "boolean", + "description": "Specifies whether or not to use bwa as a fallback aligner in case bwamem2 throws an error.", + "fa_icon": "fas fa-toggle-on" + }, "platform": { "type": "string", "default": "illumina", From 54e957cefe1daebf219c65154b5ca95ce74a4052 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 8 May 2024 14:00:59 +0000 Subject: [PATCH 44/80] Template update for nf-core/tools version 2.14.0 --- .editorconfig | 6 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 10 +- .github/workflows/awstest.yml | 12 +- .github/workflows/ci.yml | 4 +- .github/workflows/download_pipeline.yml | 22 ++- .github/workflows/fix-linting.yml | 6 +- .github/workflows/linting.yml | 18 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 6 +- .nf-core.yml | 1 + .pre-commit-config.yaml | 3 + CHANGELOG.md | 2 +- README.md | 2 +- conf/base.config | 3 - conf/modules.config | 8 - conf/test.config | 2 +- conf/test_full.config | 2 +- docs/usage.md | 2 + modules.json | 4 +- modules/nf-core/fastqc/main.nf | 6 + nextflow.config | 178 +++++++++--------- nextflow_schema.json | 7 + pyproject.toml | 15 -- .../utils_nfcore_raredisease_pipeline/main.nf | 16 +- .../nf-core/utils_nfcore_pipeline/main.nf | 8 +- workflows/raredisease.nf | 46 +++-- 27 files changed, 222 insertions(+), 171 deletions(-) delete mode 100644 pyproject.toml diff --git a/.editorconfig b/.editorconfig index dd9ffa53..72dda289 100644 --- a/.editorconfig +++ b/.editorconfig @@ -28,10 +28,6 @@ indent_style = unset [/assets/email*] indent_size = unset -# ignore Readme -[README.md] -indent_style = unset - -# ignore python +# ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 23097c52..fc4dc8ad 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rare - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/raredisease/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/raredisease _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 2919151f..6026e464 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -8,12 +8,12 @@ on: types: [published] workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS full tests if: github.repository == 'nf-core/raredisease' runs-on: ubuntu-latest steps: - - name: Launch workflow via tower + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) @@ -33,7 +33,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 4ed98bab..7a710d67 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -5,13 +5,13 @@ name: nf-core AWS test on: workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS tests if: github.repository == 'nf-core/raredisease' runs-on: ubuntu-latest steps: - # Launch workflow using Tower CLI tool action - - name: Launch workflow via tower + # Launch workflow using Seqera Platform CLI tool action + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} @@ -27,7 +27,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index e56bcd29..46a43e64 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,10 +28,10 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 08622fd5..2d20d644 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -14,6 +14,8 @@ on: pull_request: types: - opened + - edited + - synchronize branches: - master pull_request_target: @@ -28,11 +30,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: @@ -65,8 +70,17 @@ jobs: - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} - - name: Run the downloaded pipeline + - name: Run the downloaded pipeline (stub) + id: stub_run_pipeline + continue-on-error: true env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + - name: Run the downloaded pipeline (stub run not supported) + id: run_pipeline + if: ${{ job.steps.stub_run_pipeline.status == failure() }} + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 114b102d..4dbed09c 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -32,9 +32,9 @@ jobs: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} # Install and run pre-commit - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" - name: Install pre-commit run: pip install pre-commit diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e1876..a3fb2541 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,12 +14,12 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Set up Python 3.12 + uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" cache: "pip" - name: Install pre-commit @@ -32,14 +32,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - name: Install dependencies @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index b706875f..40acc23f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 + uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index d468aeaa..03ecfcf7 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT + echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: @@ -25,13 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} - ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.10" - name: Install dependencies diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc81..d6daa403 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,2 @@ repository_type: pipeline +nf_core_version: "2.14.0" diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index af57081f..4dc0f1dc 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -3,6 +3,9 @@ repos: rev: "v3.1.0" hooks: - id: prettier + additional_dependencies: + - prettier@3.2.5 + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python rev: "2.7.3" hooks: diff --git a/CHANGELOG.md b/CHANGELOG.md index a1aaaaa1..ec64267b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.2.0dev - [date] +## v2.1.0dev - [date] Initial release of nf-core/raredisease, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index bf3b4c0c..63a3c99b 100644 --- a/README.md +++ b/README.md @@ -13,7 +13,7 @@ [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/raredisease) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/raredisease) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23raredisease-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/raredisease)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/conf/base.config b/conf/base.config index 9a86068f..806d4b89 100644 --- a/conf/base.config +++ b/conf/base.config @@ -59,7 +59,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/conf/modules.config b/conf/modules.config index e3ea8fa6..d203d2b6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,14 +22,6 @@ process { ext.args = '--quiet' } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/conf/test.config b/conf/test.config index 49fc7560..6bf4a9c9 100644 --- a/conf/test.config +++ b/conf/test.config @@ -22,7 +22,7 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' // Genome references genome = 'R64-1-1' diff --git a/conf/test_full.config b/conf/test_full.config index a33b4721..9dd10b7f 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -17,7 +17,7 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' // Genome references genome = 'R64-1-1' diff --git a/docs/usage.md b/docs/usage.md index e710e34e..e3c24bc4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -156,6 +156,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) - `apptainer` - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) +- `wave` + - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). - `conda` - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. diff --git a/modules.json b/modules.json index b773a78a..886ce742 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["modules"] }, "multiqc": { @@ -26,7 +26,7 @@ }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9e19a74c..d79f1c86 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -25,6 +25,11 @@ process FASTQC { def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // FastQC memory value allowed range (100 - 10000) + def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) + """ printf "%s %s\\n" $rename_to | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name @@ -33,6 +38,7 @@ process FASTQC { fastqc \\ $args \\ --threads $task.cpus \\ + --memory $fastqc_memory \\ $renamed_files cat <<-END_VERSIONS > versions.yml diff --git a/nextflow.config b/nextflow.config index 5469bb1b..15f5b2f3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,7 +16,8 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - fasta = null// MultiQC options + + // MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -24,15 +25,16 @@ params { multiqc_methods_description = null // Boilerplate options - outdir = null - publish_dir_mode = 'copy' - email = null - email_on_fail = null - plaintext_email = false - monochrome_logs = false - hook_url = null - help = false - version = false + outdir = null + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + hook_url = null + help = false + version = false + pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options config_profile_name = null @@ -68,103 +70,109 @@ try { } // Load nf-core/raredisease custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! -// try { -// includeConfig "${params.custom_config_base}/pipeline/raredisease.config" -// } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/raredisease profiles: ${params.custom_config_base}/pipeline/raredisease.config") -// } +try { + includeConfig "${params.custom_config_base}/pipeline/raredisease.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config/raredisease profiles: ${params.custom_config_base}/pipeline/raredisease.config") +} profiles { debug { - dumpHashes = true - process.beforeScript = 'echo $HOSTNAME' - cleanup = false + dumpHashes = true + process.beforeScript = 'echo $HOSTNAME' + cleanup = false nextflow.enable.configProcessNamesValidation = true } conda { - conda.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - channels = ['conda-forge', 'bioconda', 'defaults'] - apptainer.enabled = false + conda.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + conda.channels = ['conda-forge', 'bioconda', 'defaults'] + apptainer.enabled = false } mamba { - conda.enabled = true - conda.useMamba = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + conda.enabled = true + conda.useMamba = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } docker { - docker.enabled = true - conda.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { - singularity.enabled = true - singularity.autoMounts = true - conda.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + singularity.enabled = true + singularity.autoMounts = true + conda.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } podman { - podman.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + podman.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } shifter { - shifter.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + shifter.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } charliecloud { - charliecloud.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - apptainer.enabled = false + charliecloud.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + apptainer.enabled = false } apptainer { - apptainer.enabled = true - apptainer.autoMounts = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + apptainer.enabled = true + apptainer.autoMounts = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + wave { + apptainer.ociAutoPull = true + singularity.ociAutoPull = true + wave.enabled = true + wave.freeze = true + wave.strategy = 'conda,container' } gitpod { - executor.name = 'local' - executor.cpus = 4 - executor.memory = 8.GB + executor.name = 'local' + executor.cpus = 4 + executor.memory = 8.GB } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } @@ -231,7 +239,7 @@ manifest { description = """call and score variants from WGS/WES of rare disease patients""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.2.0dev' + version = '2.1.0dev' doi = '' } diff --git a/nextflow_schema.json b/nextflow_schema.json index 2077e3bd..eebcbad7 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -265,6 +265,13 @@ "description": "Validation of parameters in lenient more.", "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + }, + "pipelines_testdata_base_path": { + "type": "string", + "fa_icon": "far fa-check-circle", + "description": "Base URL or local path to location of pipeline test dataset files", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", + "hidden": true } } } diff --git a/pyproject.toml b/pyproject.toml deleted file mode 100644 index 56110621..00000000 --- a/pyproject.toml +++ /dev/null @@ -1,15 +0,0 @@ -# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. -# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.ruff] -line-length = 120 -target-version = "py38" -cache-dir = "~/.cache/ruff" - -[tool.ruff.lint] -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] - -[tool.ruff.lint.isort] -known-first-party = ["nf_core"] - -[tool.ruff.lint.per-file-ignores] -"__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf index 3ec266c7..b95a50e7 100644 --- a/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_raredisease_pipeline/main.nf @@ -140,6 +140,10 @@ workflow PIPELINE_COMPLETION { imNotification(summary_params, hook_url) } } + + workflow.onError { + log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting" + } } /* @@ -230,8 +234,16 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["manifest_map"] = workflow.manifest.toMap() // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
  • " + if (meta.manifest_map.doi) { + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + def temp_doi_ref = "" + String[] manifest_doi = meta.manifest_map.doi.tokenize(",") + for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) + } else meta["doi_text"] = "" + meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
  • " // Tool references meta["tool_citations"] = "" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index a8b55d6f..14558c39 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) { // Citation string for pipeline // def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + - " ${workflow.manifest.doi}\n\n" + + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index c9dd37ed..27bcebe2 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -40,22 +40,44 @@ workflow RAREDISEASE { // Collate and save software versions // softwareVersionsToYAML(ch_versions) - .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) - .set { ch_collated_versions } + .collectFile( + storeDir: "${params.outdir}/pipeline_info", + name: 'nf_core_pipeline_software_mqc_versions.yml', + sort: true, + newLine: true + ).set { ch_collated_versions } // // MODULE: MultiQC // - ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) - ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) + ch_multiqc_config = Channel.fromPath( + "$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? + Channel.fromPath(params.multiqc_config, checkIfExists: true) : + Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? + Channel.fromPath(params.multiqc_logo, checkIfExists: true) : + Channel.empty() + + summary_params = paramsSummaryMap( + workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? + file(params.multiqc_methods_description, checkIfExists: true) : + file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value( + methodsDescriptionText(ch_multiqc_custom_methods_description)) + + ch_multiqc_files = ch_multiqc_files.mix( + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix( + ch_methods_description.collectFile( + name: 'methods_description_mqc.yaml', + sort: true + ) + ) MULTIQC ( ch_multiqc_files.collect(), From b55545c00da19229686bfd99b6b1c1688f3c86a1 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 9 May 2024 11:42:50 +0000 Subject: [PATCH 45/80] Template update for nf-core/tools version 2.14.1 --- .github/workflows/linting.yml | 1 - .nf-core.yml | 2 +- 2 files changed, 1 insertion(+), 2 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index a3fb2541..1fcafe88 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -20,7 +20,6 @@ jobs: uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.12" - cache: "pip" - name: Install pre-commit run: pip install pre-commit diff --git a/.nf-core.yml b/.nf-core.yml index d6daa403..e0b85a77 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,2 +1,2 @@ repository_type: pipeline -nf_core_version: "2.14.0" +nf_core_version: "2.14.1" From 2ca958fd17f2dbc9ffc9cbe41f19880367b26c82 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 14 May 2024 13:13:28 +0200 Subject: [PATCH 46/80] update test one sample --- conf/test_one_sample.config | 50 ++++++++++++++++++------------------- 1 file changed, 25 insertions(+), 25 deletions(-) diff --git a/conf/test_one_sample.config b/conf/test_one_sample.config index 404fe607..4f641aac 100644 --- a/conf/test_one_sample.config +++ b/conf/test_one_sample.config @@ -34,33 +34,33 @@ params { skip_peddy = true // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/testdata/samplesheet_single.csv' + input = params.pipelines_testdata_base_path + 'raredisease/testdata/samplesheet_single.csv' // Genome references - fasta = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta" - fai = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reference.fasta.fai" + fasta = params.pipelines_testdata_base_path + 'raredisease/reference/reference.fasta' + fai = params.pipelines_testdata_base_path + 'raredisease/reference/reference.fasta.fai' genome = 'GRCh37' - gnomad_af = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/gnomad_reformated.tab.gz" - intervals_wgs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target_wgs.interval_list" - intervals_y = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/targetY.interval_list" - known_dbsnp = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/dbsnp_-138-.vcf.gz" - ml_model = "https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model" - mobile_element_references = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/mobile_element_references.tsv" - mobile_element_svdb_annotations = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" - reduced_penetrance = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/reduced_penetrance.tsv" - score_config_mt = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" - score_config_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_snv.ini" - score_config_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/rank_model_sv.ini" - svdb_query_dbs = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/svdb_querydb_files.csv" - target_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/target.bed" - variant_catalog = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_catalog.json" - vcfanno_lua = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_functions.lua" - vcfanno_resources = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_resources.txt" - vcfanno_toml = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vcfanno_config.toml" - variant_consequences_snv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" - variant_consequences_sv = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/variant_consequences_v2.txt" - vep_cache = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_cache_and_plugins.tar.gz" - vep_filters = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/hgnc.txt" + gnomad_af = params.pipelines_testdata_base_path + 'raredisease/reference/gnomad_reformated.tab.gz' + intervals_wgs = params.pipelines_testdata_base_path + 'raredisease/reference/target_wgs.interval_list' + intervals_y = params.pipelines_testdata_base_path + 'raredisease/reference/targetY.interval_list' + known_dbsnp = params.pipelines_testdata_base_path + 'raredisease/reference/dbsnp_-138-.vcf.gz' + ml_model = 'https://s3.amazonaws.com/sentieon-release/other/SentieonDNAscopeModel1.0.model' + mobile_element_references = params.pipelines_testdata_base_path + 'raredisease/reference/mobile_element_references.tsv' + mobile_element_svdb_annotations = params.pipelines_testdata_base_path + 'raredisease/reference/svdb_querydb_files.csv' + reduced_penetrance = params.pipelines_testdata_base_path + 'raredisease/reference/reduced_penetrance.tsv' + score_config_mt = params.pipelines_testdata_base_path + 'raredisease/reference/rank_model_snv.ini' + score_config_snv = params.pipelines_testdata_base_path + 'raredisease/reference/rank_model_snv.ini' + score_config_sv = params.pipelines_testdata_base_path + 'raredisease/reference/rank_model_sv.ini' + svdb_query_dbs = params.pipelines_testdata_base_path + 'raredisease/reference/svdb_querydb_files.csv' + target_bed = params.pipelines_testdata_base_path + 'raredisease/reference/target.bed' + variant_catalog = params.pipelines_testdata_base_path + 'raredisease/reference/variant_catalog.json' + vcfanno_lua = params.pipelines_testdata_base_path + 'raredisease/reference/vcfanno_functions.lua' + vcfanno_resources = params.pipelines_testdata_base_path + 'raredisease/reference/vcfanno_resources.txt' + vcfanno_toml = params.pipelines_testdata_base_path + 'raredisease/reference/vcfanno_config.toml' + variant_consequences_snv = params.pipelines_testdata_base_path + 'raredisease/reference/variant_consequences_v2.txt' + variant_consequences_sv = params.pipelines_testdata_base_path + 'raredisease/reference/variant_consequences_v2.txt' + vep_cache = params.pipelines_testdata_base_path + 'raredisease/reference/vep_cache_and_plugins.tar.gz' + vep_filters = params.pipelines_testdata_base_path + 'raredisease/reference/hgnc.txt' vep_cache_version = 107 - vep_plugin_files = "https://raw.githubusercontent.com/nf-core/test-datasets/raredisease/reference/vep_files.csv" + vep_plugin_files = params.pipelines_testdata_base_path + 'raredisease/reference/vep_files.csv' } From d605f7e02cec12532f37d1f3bac762de05060736 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 14 May 2024 13:38:49 +0200 Subject: [PATCH 47/80] fix lint error --- .nf-core.yml | 3 +-- nextflow_schema.json | 3 +-- 2 files changed, 2 insertions(+), 4 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 6d22d4b4..070882cb 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,5 @@ lint: - files_exist: - - conf/modules.config + modules_config: False files_unchanged: - .github/CONTRIBUTING.md - .github/PULL_REQUEST_TEMPLATE.md diff --git a/nextflow_schema.json b/nextflow_schema.json index 178afd2d..8e5ed746 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -27,8 +27,7 @@ "type": "string", "format": "directory-path", "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open", - "default": "results" + "fa_icon": "fas fa-folder-open" }, "email": { "type": "string", From 9838708e245fbf9d4f8a6fd81c7e4a7f6dd23256 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 14 May 2024 13:42:55 +0200 Subject: [PATCH 48/80] fix lint --- .nf-core.yml | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/.nf-core.yml b/.nf-core.yml index 070882cb..8d084d3b 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,10 +1,12 @@ lint: - modules_config: False + files_exist: + - conf/modules.config files_unchanged: - .github/CONTRIBUTING.md - .github/PULL_REQUEST_TEMPLATE.md - docs/images/nf-core-raredisease_logo_dark.png - docs/images/nf-core-raredisease_logo_light.png - assets/nf-core-raredisease_logo_light.png + modules_config: False repository_type: pipeline nf_core_version: "2.14.1" From 03b0e9d40d3a8cd94ffa2dad572aaec094174edb Mon Sep 17 00:00:00 2001 From: Sima Rahimi <54620700+sima-r@users.noreply.github.com> Date: Thu, 16 May 2024 10:26:16 +0200 Subject: [PATCH 49/80] changed valid values for sex This is now compatible with the PED file format and documentation --- assets/schema_input.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 7bcd1812..38249daa 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -40,9 +40,9 @@ ] }, "sex": { - "type": "integer", + "type": "string", "meta": ["sex"], - "enum": [0, 1, 2], + "enum": ["1", "2", "other"], "errorMessage": "Sex must be provided and cannot contain spaces" }, "phenotype": { From 9da1d8d8a92f6605546610cf86b6c2d169f21f3c Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 17 May 2024 10:41:36 +0200 Subject: [PATCH 50/80] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index d054ab79..2e74f569 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` ### `Changed` + - Refactored config files [#538](https://github.com/nf-core/raredisease/pull/538) - Refactored mobile element annotation subworkflow files [#538](https://github.com/nf-core/raredisease/pull/538) From 91fc58e3a39ab8ff37eb9154f5ee48f155bef579 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 17 May 2024 13:14:52 +0200 Subject: [PATCH 51/80] update changelog --- CHANGELOG.md | 2 ++ nextflow_schema.json | 1 + 2 files changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2e74f569..44e6f516 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,6 +7,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` +- A new parameter `bwa_as_fallback` to switch aligner to bwa in case bwamem2 fails [#551](https://github.com/nf-core/raredisease/pull/551) + ### `Changed` - Refactored config files [#538](https://github.com/nf-core/raredisease/pull/538) diff --git a/nextflow_schema.json b/nextflow_schema.json index 0c26050c..674c19c8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -468,6 +468,7 @@ "bwa_as_fallback": { "type": "boolean", "description": "Specifies whether or not to use bwa as a fallback aligner in case bwamem2 throws an error.", + "help_text": "errorStrategy needs to be set to ignore for the bwamem2 process for the fallback to work. Turned off by default.", "fa_icon": "fas fa-toggle-on" }, "platform": { From 2f42dd03b7e038d992425e2c8d1d063dfeb399ff Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 17 May 2024 19:28:20 +0200 Subject: [PATCH 52/80] refactor mito --- conf/modules/align_MT.config | 6 -- conf/modules/call_repeat_expansions.config | 4 - conf/modules/prepare_references.config | 10 +-- conf/modules/qc_bam.config | 8 +- nextflow.config | 1 + nextflow_schema.json | 5 ++ subworkflows/local/align.nf | 87 ++++++++++--------- subworkflows/local/alignment/align_MT.nf | 16 ++-- subworkflows/local/call_snv.nf | 81 +++++++++-------- .../local/call_structural_variants.nf | 6 +- subworkflows/local/qc_bam.nf | 22 ++--- workflows/raredisease.nf | 22 ++--- 12 files changed, 139 insertions(+), 129 deletions(-) diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index 1bd88f14..6ec96d0d 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -19,19 +19,16 @@ process { withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } ext.prefix = { "${meta.id}_sorted" } } withName: '.*ALIGN_MT:BWA_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } ext.prefix = { "${meta.id}_sorted" } } withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes"))} ext.prefix = { "${meta.id}_sorted" } } @@ -70,19 +67,16 @@ process { withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } ext.prefix = { "${meta.id}_sorted_shifted" } } withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes"))} ext.prefix = { "${meta.id}_sorted_shifted" } } withName: '.*ALIGN_MT_SHIFT:BWA_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } - ext.when = { !(params.analysis_type.equals("wes")) } ext.prefix = { "${meta.id}_sorted_shifted" } } diff --git a/conf/modules/call_repeat_expansions.config b/conf/modules/call_repeat_expansions.config index 002ddffb..a3c140a0 100644 --- a/conf/modules/call_repeat_expansions.config +++ b/conf/modules/call_repeat_expansions.config @@ -16,10 +16,6 @@ // process { - withName: '.*CALL_REPEAT_EXPANSIONS:.*' { - ext.when = { params.analysis_type != "wes" } - } - withName: '.*CALL_REPEAT_EXPANSIONS:EXPANSIONHUNTER' { ext.args = { ("${meta.sex}" == '1') ? '--sex male' : '--sex female' } ext.prefix = { "${meta.id}_exphunter" } diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 103a1ad7..efcea90c 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -30,7 +30,7 @@ process { } withName: '.*PREPARE_REFERENCES:BWAMEM2_INDEX_MT_SHIFT' { - ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwamem2"} + ext.when = { (params.analysis_type.equals("wgs") || params.run_mt_for_wes) && params.aligner == "bwamem2"} } withName: '.*PREPARE_REFERENCES:SENTIEON_BWAINDEX_GENOME' { @@ -38,11 +38,11 @@ process { } withName: '.*PREPARE_REFERENCES:SENTIEON_BWAINDEX_MT_SHIFT' { - ext.when = { !(params.analysis_type == "wes") && params.aligner == "sentieon"} + ext.when = { (params.analysis_type.equals("wgs") || params.run_mt_for_wes) && params.aligner == "sentieon"} } withName: '.*PREPARE_REFERENCES:BWA_INDEX_MT_SHIFT' { - ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwa"} + ext.when = { (params.analysis_type.equals("wgs") || params.run_mt_for_wes) && params.aligner == "bwa"} } withName: '.*PREPARE_REFERENCES:BWA_INDEX_GENOME' { @@ -63,7 +63,7 @@ process { } withName: '.*PREPARE_REFERENCES:SAMTOOLS_FAIDX_MT_SHIFT' { - ext.when = { !(params.analysis_type == "wes")} + ext.when = { (params.analysis_type.equals("wgs") || params.run_mt_for_wes) } } withName: '.*PREPARE_REFERENCES:GATK_SD' { @@ -75,7 +75,7 @@ process { } withName: '.*PREPARE_REFERENCES:GATK_SD_MT_SHIFT' { - ext.when = { !(params.analysis_type == "wes")} + ext.when = { (params.analysis_type.equals("wgs") || params.run_mt_for_wes)} } withName: '.*PREPARE_REFERENCES:TABIX_DBSNP' { diff --git a/conf/modules/qc_bam.config b/conf/modules/qc_bam.config index d57670c7..7d88c095 100644 --- a/conf/modules/qc_bam.config +++ b/conf/modules/qc_bam.config @@ -74,23 +74,23 @@ process { withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS' { ext.args = "--TMP_DIR ." - ext.when = { params.analysis_type.equals("wgs") && !params.aligner.equals("sentieon") } + ext.when = { !params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics" } } withName: '.*QC_BAM:PICARD_COLLECTWGSMETRICS_Y' { ext.args = "--TMP_DIR ." - ext.when = { params.analysis_type.equals("wgs") && !params.aligner.equals("sentieon") } + ext.when = { !params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics_y" } } withName: '.*QC_BAM:SENTIEON_WGSMETRICS' { - ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("sentieon") } + ext.when = { params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics" } } withName: '.*QC_BAM:SENTIEON_WGSMETRICS_Y' { - ext.when = { params.analysis_type.equals("wgs") && params.aligner.equals("sentieon") } + ext.when = { params.aligner.equals("sentieon") } ext.prefix = { "${meta.id}_wgsmetrics_y" } } } diff --git a/nextflow.config b/nextflow.config index a0dcaa43..e4d19a56 100644 --- a/nextflow.config +++ b/nextflow.config @@ -23,6 +23,7 @@ params { // Main options analysis_type = 'wgs' bait_padding = 100 + run_mt_for_wes = false run_rtgvcfeval = false save_mapped_as_cram = false skip_eklipse = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 8e5ed746..72d5496c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -479,6 +479,11 @@ "fa_icon": "fas fa-align-center", "enum": ["xy", "hetx", "sry"] }, + "run_mt_for_wes": { + "type": "boolean", + "description": "Specifies whether to run mitochondrial analysis for wes samples", + "fa_icon": "fas fa-toggle-on" + }, "run_rtgvcfeval": { "type": "boolean", "description": "Specifies whether to run rtgtools' vcfeval", diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 51bbf3fb..da1c7ab6 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -26,12 +26,18 @@ workflow ALIGN { val_platform // string: [mandatory] illumina or a different technology main: - ch_versions = Channel.empty() - ch_fastp_json = Channel.empty() - ch_bwamem2_bam = Channel.empty() - ch_sentieon_bam = Channel.empty() - ch_bwamem2_bai = Channel.empty() - ch_sentieon_bai = Channel.empty() + ch_bwamem2_bam = Channel.empty() + ch_bwamem2_bai = Channel.empty() + ch_fastp_json = Channel.empty() + ch_mt_bam_bai = Channel.empty() + ch_mt_marked_bam = Channel.empty() + ch_mt_marked_bai = Channel.empty() + ch_mtshift_bam_bai = Channel.empty() + ch_mtshift_marked_bam = Channel.empty() + ch_mtshift_marked_bai = Channel.empty() + ch_sentieon_bam = Channel.empty() + ch_sentieon_bai = Channel.empty() + ch_versions = Channel.empty() if (!params.skip_fastp) { FASTP (ch_reads, [], false, false) @@ -70,48 +76,49 @@ workflow ALIGN { ch_genome_bam_bai = ch_genome_marked_bam.join(ch_genome_marked_bai, failOnMismatch:true, failOnDuplicate:true) // PREPARING READS FOR MT ALIGNMENT - CONVERT_MT_BAM_TO_FASTQ ( - ch_genome_bam_bai, - ch_genome_fasta, - ch_genome_fai, - ch_genome_dictionary - ) - - ALIGN_MT ( - CONVERT_MT_BAM_TO_FASTQ.out.fastq, - CONVERT_MT_BAM_TO_FASTQ.out.bam, - ch_genome_bwaindex, - ch_genome_bwamem2index, - ch_genome_fasta, - ch_genome_dictionary, - ch_genome_fai - ) + if (params.analysis_type.equals("wgs") || params.run_mt_for_wes) { + CONVERT_MT_BAM_TO_FASTQ ( + ch_genome_bam_bai, + ch_genome_fasta, + ch_genome_fai, + ch_genome_dictionary + ) - ALIGN_MT_SHIFT ( - CONVERT_MT_BAM_TO_FASTQ.out.fastq, - CONVERT_MT_BAM_TO_FASTQ.out.bam, - ch_mtshift_bwaindex, - ch_mtshift_bwamem2index, - ch_mtshift_fasta, - ch_mtshift_dictionary, - ch_mtshift_fai - ) + ALIGN_MT ( + CONVERT_MT_BAM_TO_FASTQ.out.fastq, + CONVERT_MT_BAM_TO_FASTQ.out.bam, + ch_genome_bwaindex, + ch_genome_bwamem2index, + ch_genome_fasta, + ch_genome_dictionary, + ch_genome_fai + ) - ch_mt_marked_bam = ALIGN_MT.out.marked_bam - ch_mt_marked_bai = ALIGN_MT.out.marked_bai - ch_mt_bam_bai = ch_mt_marked_bam.join(ch_mt_marked_bai, failOnMismatch:true, failOnDuplicate:true) + ALIGN_MT_SHIFT ( + CONVERT_MT_BAM_TO_FASTQ.out.fastq, + CONVERT_MT_BAM_TO_FASTQ.out.bam, + ch_mtshift_bwaindex, + ch_mtshift_bwamem2index, + ch_mtshift_fasta, + ch_mtshift_dictionary, + ch_mtshift_fai + ) - ch_mtshift_marked_bam = ALIGN_MT_SHIFT.out.marked_bam - ch_mtshift_marked_bai = ALIGN_MT_SHIFT.out.marked_bai - ch_mtshift_bam_bai = ch_mtshift_marked_bam.join(ch_mtshift_marked_bai, failOnMismatch:true, failOnDuplicate:true) + ch_mt_marked_bam = ALIGN_MT.out.marked_bam + ch_mt_marked_bai = ALIGN_MT.out.marked_bai + ch_mt_bam_bai = ch_mt_marked_bam.join(ch_mt_marked_bai, failOnMismatch:true, failOnDuplicate:true) + ch_mtshift_marked_bam = ALIGN_MT_SHIFT.out.marked_bam + ch_mtshift_marked_bai = ALIGN_MT_SHIFT.out.marked_bai + ch_mtshift_bam_bai = ch_mtshift_marked_bam.join(ch_mtshift_marked_bai, failOnMismatch:true, failOnDuplicate:true) + ch_versions = ch_versions.mix(ALIGN_MT.out.versions, + ALIGN_MT_SHIFT.out.versions, + CONVERT_MT_BAM_TO_FASTQ.out.versions) + } if (params.save_mapped_as_cram) { SAMTOOLS_VIEW( ch_genome_bam_bai, ch_genome_fasta, [] ) ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions) } - ch_versions = ch_versions.mix(ALIGN_MT.out.versions, - ALIGN_MT_SHIFT.out.versions, - CONVERT_MT_BAM_TO_FASTQ.out.versions) emit: fastp_json = ch_fastp_json // channel: [ val(meta), path(json) ] diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf index 8f7930c7..1bb5c56f 100644 --- a/subworkflows/local/alignment/align_MT.nf +++ b/subworkflows/local/alignment/align_MT.nf @@ -23,25 +23,21 @@ workflow ALIGN_MT { main: ch_versions = Channel.empty() - ch_bwa_bam = Channel.empty() - ch_bwamem2_bam = Channel.empty() - ch_sentieon_bam = Channel.empty() if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) - ch_bwamem2_bam = BWAMEM2_MEM_MT.out.bam + ch_align = BWAMEM2_MEM_MT.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM_MT.out.versions.first()) } else if (params.aligner.equals("sentieon")) { SENTIEON_BWAMEM_MT ( ch_fastq, ch_bwaindex, ch_fasta, ch_fai ) - ch_sentieon_bam = SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] } - ch_versions = ch_versions.mix(SENTIEON_BWAMEM_MT.out.versions.first()) + ch_align = SENTIEON_BWAMEM_MT.out.bam_and_bai.map{ meta, bam, bai -> [meta, bam] } + ch_versions = ch_versions.mix(SENTIEON_BWAMEM_MT.out.versions.first()) } else if (params.aligner.equals("bwa")) { BWA_MEM_MT ( ch_fastq, ch_bwaindex, true ) - ch_bwa_bam = BWA_MEM_MT.out.bam - ch_versions = ch_versions.mix(BWA_MEM_MT.out.versions.first()) + ch_align = BWA_MEM_MT.out.bam + ch_versions = ch_versions.mix(BWA_MEM_MT.out.versions.first()) } - Channel.empty() - .mix(ch_bwamem2_bam, ch_sentieon_bam, ch_bwa_bam) + ch_align .join(ch_ubam, failOnMismatch:true, failOnDuplicate:true) .set {ch_bam_ubam} diff --git a/subworkflows/local/call_snv.nf b/subworkflows/local/call_snv.nf index 72c91ecf..48bc500a 100644 --- a/subworkflows/local/call_snv.nf +++ b/subworkflows/local/call_snv.nf @@ -38,6 +38,8 @@ workflow CALL_SNV { ch_deepvar_tbi = Channel.empty() ch_deepvar_gvcf = Channel.empty() ch_deepvar_gtbi = Channel.empty() + ch_mt_vcf = Channel.empty() + ch_mt_tabix = Channel.empty() ch_sentieon_vcf = Channel.empty() ch_sentieon_tbi = Channel.empty() ch_sentieon_gvcf = Channel.empty() @@ -93,46 +95,49 @@ workflow CALL_SNV { ch_genome_tabix = GATK4_SELECTVARIANTS.out.tbi ch_genome_vcf_tabix = ch_genome_vcf.join(ch_genome_tabix, failOnMismatch:true, failOnDuplicate:true) - CALL_SNV_MT( - ch_mt_bam_bai, - ch_genome_fasta, - ch_genome_fai, - ch_genome_dictionary, - ch_mt_intervals - ) - - CALL_SNV_MT_SHIFT( - ch_mtshift_bam_bai, - ch_mtshift_fasta, - ch_mtshift_fai, - ch_mtshift_dictionary, - ch_mtshift_intervals - ) + if (params.analysis_type.equals("wgs") || params.run_mt_for_wes) { + CALL_SNV_MT( + ch_mt_bam_bai, + ch_genome_fasta, + ch_genome_fai, + ch_genome_dictionary, + ch_mt_intervals + ) - POSTPROCESS_MT_CALLS( - CALL_SNV_MT.out.vcf, - CALL_SNV_MT_SHIFT.out.vcf, - ch_genome_fasta, - ch_genome_dictionary, - ch_genome_fai, - ch_mtshift_backchain, - ch_case_info, - ch_foundin_header, - ch_genome_chrsizes - ) + CALL_SNV_MT_SHIFT( + ch_mtshift_bam_bai, + ch_mtshift_fasta, + ch_mtshift_fai, + ch_mtshift_dictionary, + ch_mtshift_intervals + ) - ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) - ch_versions = ch_versions.mix(CALL_SNV_MT_SHIFT.out.versions) - ch_versions = ch_versions.mix(POSTPROCESS_MT_CALLS.out.versions) - ch_versions = ch_versions.mix(GATK4_SELECTVARIANTS.out.versions) + POSTPROCESS_MT_CALLS( + CALL_SNV_MT.out.vcf, + CALL_SNV_MT_SHIFT.out.vcf, + ch_genome_fasta, + ch_genome_dictionary, + ch_genome_fai, + ch_mtshift_backchain, + ch_case_info, + ch_foundin_header, + ch_genome_chrsizes + ) + ch_mt_vcf = POSTPROCESS_MT_CALLS.out.vcf + ch_mt_tabix = POSTPROCESS_MT_CALLS.out.tbi + ch_versions = ch_versions.mix(CALL_SNV_MT.out.versions) + ch_versions = ch_versions.mix(CALL_SNV_MT_SHIFT.out.versions) + ch_versions = ch_versions.mix(POSTPROCESS_MT_CALLS.out.versions) + ch_versions = ch_versions.mix(GATK4_SELECTVARIANTS.out.versions) + } emit: - genome_vcf = ch_genome_vcf // channel: [ val(meta), path(vcf) ] - genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] - genome_vcf_tabix = ch_genome_vcf_tabix // channel: [ val(meta), path(vcf), path(tbi) ] - genome_gvcf = ch_gvcf // channel: [ val(meta), path(gvcf) ] - genome_gtabix = ch_gtabix // channel: [ val(meta), path(gtbi) ] - mt_vcf = POSTPROCESS_MT_CALLS.out.vcf // channel: [ val(meta), path(vcf) ] - mt_tabix = POSTPROCESS_MT_CALLS.out.tbi // channel: [ val(meta), path(tbi) ] - versions = ch_versions // channel: [ path(versions.yml) ] + genome_vcf = ch_genome_vcf // channel: [ val(meta), path(vcf) ] + genome_tabix = ch_genome_tabix // channel: [ val(meta), path(tbi) ] + genome_vcf_tabix = ch_genome_vcf_tabix // channel: [ val(meta), path(vcf), path(tbi) ] + genome_gvcf = ch_gvcf // channel: [ val(meta), path(gvcf) ] + genome_gtabix = ch_gtabix // channel: [ val(meta), path(gtbi) ] + mt_vcf = ch_mt_vcf // channel: [ val(meta), path(vcf) ] + mt_tabix = ch_mt_tabix // channel: [ val(meta), path(tbi) ] + versions = ch_versions // channel: [ path(versions.yml) ] } diff --git a/subworkflows/local/call_structural_variants.nf b/subworkflows/local/call_structural_variants.nf index f590bb60..76f40af5 100644 --- a/subworkflows/local/call_structural_variants.nf +++ b/subworkflows/local/call_structural_variants.nf @@ -57,7 +57,10 @@ workflow CALL_STRUCTURAL_VARIANTS { .collect{it[1]} .set { cnvnator_vcf } - CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) + if (params.analysis_type.equals("wgs") || params.run_mt_for_wes) { + CALL_SV_MT (ch_mt_bam_bai, ch_genome_fasta) + ch_versions = ch_versions.mix(CALL_SV_MT.out.versions) + } //merge if (params.skip_germlinecnvcaller) { @@ -85,7 +88,6 @@ workflow CALL_STRUCTURAL_VARIANTS { ch_versions = ch_versions.mix(CALL_SV_CNVNATOR.out.versions) ch_versions = ch_versions.mix(CALL_SV_MANTA.out.versions) - ch_versions = ch_versions.mix(CALL_SV_MT.out.versions) ch_versions = ch_versions.mix(CALL_SV_TIDDIT.out.versions) ch_versions = ch_versions.mix(TABIX_TABIX.out.versions) ch_versions = ch_versions.mix(SVDB_MERGE.out.versions) diff --git a/subworkflows/local/qc_bam.nf b/subworkflows/local/qc_bam.nf index b1ce5141..b8bc8af6 100644 --- a/subworkflows/local/qc_bam.nf +++ b/subworkflows/local/qc_bam.nf @@ -31,6 +31,8 @@ workflow QC_BAM { ngsbits_samplegender_method // channel [val(method)] main: + ch_cov = Channel.empty() + ch_cov_y = Channel.empty() ch_versions = Channel.empty() ch_qualimap = Channel.empty() @@ -58,17 +60,19 @@ workflow QC_BAM { MOSDEPTH (ch_mosdepth_in, ch_genome_fasta) // COLLECT WGS METRICS - PICARD_COLLECTWGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs ) - PICARD_COLLECTWGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y ) - - SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs.map{ interval -> [[:], interval]} ) - SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y.map{ interval -> [[:], interval]} ) - + if (!params.analysis_type.equals("wes")) { + PICARD_COLLECTWGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs ) + PICARD_COLLECTWGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y ) + SENTIEON_WGSMETRICS ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_wgs.map{ interval -> [[:], interval]} ) + SENTIEON_WGSMETRICS_Y ( ch_bam_bai, ch_genome_fasta, ch_genome_fai, ch_intervals_y.map{ interval -> [[:], interval]} ) + ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) + ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) + ch_versions = ch_versions.mix(PICARD_COLLECTWGSMETRICS.out.versions.first(), SENTIEON_WGSMETRICS.out.versions.first()) + ch_versions = ch_versions.mix(PICARD_COLLECTWGSMETRICS_Y.out.versions.first(), SENTIEON_WGSMETRICS_Y.out.versions.first()) + } // Check sex NGSBITS_SAMPLEGENDER(ch_bam_bai, ch_genome_fasta, ch_genome_fai, ngsbits_samplegender_method) - ch_cov = Channel.empty().mix(PICARD_COLLECTWGSMETRICS.out.metrics, SENTIEON_WGSMETRICS.out.wgs_metrics) - ch_cov_y = Channel.empty().mix(PICARD_COLLECTWGSMETRICS_Y.out.metrics, SENTIEON_WGSMETRICS_Y.out.wgs_metrics) ch_versions = ch_versions.mix(CHROMOGRAPH_COV.out.versions.first()) ch_versions = ch_versions.mix(PICARD_COLLECTMULTIPLEMETRICS.out.versions.first()) @@ -77,8 +81,6 @@ workflow QC_BAM { ch_versions = ch_versions.mix(UCSC_WIGTOBIGWIG.out.versions.first()) ch_versions = ch_versions.mix(MOSDEPTH.out.versions.first()) ch_versions = ch_versions.mix(NGSBITS_SAMPLEGENDER.out.versions.first()) - ch_versions = ch_versions.mix(PICARD_COLLECTWGSMETRICS.out.versions.first(), SENTIEON_WGSMETRICS.out.versions.first()) - ch_versions = ch_versions.mix(PICARD_COLLECTWGSMETRICS_Y.out.versions.first(), SENTIEON_WGSMETRICS_Y.out.versions.first()) emit: multiple_metrics = PICARD_COLLECTMULTIPLEMETRICS.out.metrics // channel: [ val(meta), path(metrics) ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 8d3e0aa2..17ac7f5c 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -366,7 +366,7 @@ workflow RAREDISEASE { .set { ch_mapped } ch_versions = ch_versions.mix(ALIGN.out.versions) - if (!params.skip_mt_subsample) { + if (!params.skip_mt_subsample && (params.analysis_type.equals("wgs") || params.run_mt_for_wes)) { SUBSAMPLE_MT( ch_mapped.mt_bam_bai, params.mt_subsample_rd, @@ -396,14 +396,16 @@ workflow RAREDISEASE { // // EXPANSIONHUNTER AND STRANGER // - CALL_REPEAT_EXPANSIONS ( - ch_mapped.genome_bam_bai, - ch_variant_catalog, - ch_case_info, - ch_genome_fasta, - ch_genome_fai - ) - ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) + if (params.analysis_type.equals("wgs")) { + CALL_REPEAT_EXPANSIONS ( + ch_mapped.genome_bam_bai, + ch_variant_catalog, + ch_case_info, + ch_genome_fasta, + ch_genome_fai + ) + ch_versions = ch_versions.mix(CALL_REPEAT_EXPANSIONS.out.versions) + } // // SNV CALLING @@ -557,7 +559,7 @@ workflow RAREDISEASE { // // ANNOTATE MT SNVs // - if (!params.skip_mt_annotation) { + if (!params.skip_mt_annotation && (params.run_mt_for_wes || params.analysis_type.equals("wgs"))) { ANNOTATE_MT_SNVS ( CALL_SNV.out.mt_vcf, From 6ac690f98cd768c4897ad4d7aa9f94811362838d Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 17 May 2024 19:36:04 +0200 Subject: [PATCH 53/80] updatae changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2e74f569..69e6f072 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,6 +7,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` +- A new parameter `run_mt_for_wes` to turn on mitochondrial analysis for WES cases [#552](https://github.com/nf-core/raredisease/pull/552) + ### `Changed` - Refactored config files [#538](https://github.com/nf-core/raredisease/pull/538) From 2d5c9181d1cd8900b26bab78b37cd28151a85ee9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Fri, 17 May 2024 22:19:00 +0200 Subject: [PATCH 54/80] Update align_bwa_bwamem2.nf --- subworkflows/local/alignment/align_bwa_bwamem2.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index 40e93196..b846cad3 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -34,7 +34,7 @@ workflow ALIGN_BWA_BWAMEM2 { ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) - if (params.bwa_as_fallback.equals(true)) { + if (params.bwa_as_fallback) { ch_reads_input .join(BWAMEM2_MEM.out.bam, remainder: true) .branch { it -> From a27c043cdb7b4d40329d0555327b9d187f749e65 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 19 May 2024 11:06:34 +0200 Subject: [PATCH 55/80] add mem and index --- modules.json | 10 + modules/nf-core/bwameme/index/environment.yml | 7 + modules/nf-core/bwameme/index/main.nf | 61 +++++ modules/nf-core/bwameme/index/meta.yml | 44 ++++ .../nf-core/bwameme/index/tests/main.nf.test | 59 +++++ .../bwameme/index/tests/main.nf.test.snap | 108 ++++++++ .../bwameme/index/tests/nextflow.config | 7 + modules/nf-core/bwameme/index/tests/tags.yml | 2 + modules/nf-core/bwameme/mem/environment.yml | 11 + modules/nf-core/bwameme/mem/main.nf | 94 +++++++ modules/nf-core/bwameme/mem/meta.yml | 90 +++++++ .../nf-core/bwameme/mem/tests/main.nf.test | 239 ++++++++++++++++++ .../bwameme/mem/tests/main.nf.test.snap | 67 +++++ .../nf-core/bwameme/mem/tests/nextflow.config | 9 + modules/nf-core/bwameme/mem/tests/tags.yml | 2 + 15 files changed, 810 insertions(+) create mode 100644 modules/nf-core/bwameme/index/environment.yml create mode 100644 modules/nf-core/bwameme/index/main.nf create mode 100644 modules/nf-core/bwameme/index/meta.yml create mode 100644 modules/nf-core/bwameme/index/tests/main.nf.test create mode 100644 modules/nf-core/bwameme/index/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwameme/index/tests/nextflow.config create mode 100644 modules/nf-core/bwameme/index/tests/tags.yml create mode 100644 modules/nf-core/bwameme/mem/environment.yml create mode 100644 modules/nf-core/bwameme/mem/main.nf create mode 100644 modules/nf-core/bwameme/mem/meta.yml create mode 100644 modules/nf-core/bwameme/mem/tests/main.nf.test create mode 100644 modules/nf-core/bwameme/mem/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwameme/mem/tests/nextflow.config create mode 100644 modules/nf-core/bwameme/mem/tests/tags.yml diff --git a/modules.json b/modules.json index 2e29bd6f..e23ee838 100644 --- a/modules.json +++ b/modules.json @@ -75,6 +75,16 @@ "git_sha": "74363e1acc38eaedeede8d429477397c1a6f9e18", "installed_by": ["modules"] }, + "bwameme/index": { + "branch": "master", + "git_sha": "79480293280ff4f10f30bdea1ddd903f223f8489", + "installed_by": ["modules"] + }, + "bwameme/mem": { + "branch": "master", + "git_sha": "79480293280ff4f10f30bdea1ddd903f223f8489", + "installed_by": ["modules"] + }, "cadd": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", diff --git a/modules/nf-core/bwameme/index/environment.yml b/modules/nf-core/bwameme/index/environment.yml new file mode 100644 index 00000000..5d76ea18 --- /dev/null +++ b/modules/nf-core/bwameme/index/environment.yml @@ -0,0 +1,7 @@ +name: "bwameme_index" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::bwa-meme=1.0.6" diff --git a/modules/nf-core/bwameme/index/main.nf b/modules/nf-core/bwameme/index/main.nf new file mode 100644 index 00000000..870f494c --- /dev/null +++ b/modules/nf-core/bwameme/index/main.nf @@ -0,0 +1,61 @@ +process BWAMEME_INDEX { + tag "$fasta" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bwa-meme:1.0.6--hdcf5f25_2': + 'biocontainers/bwa-meme:1.0.6--hdcf5f25_2' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("bwameme"), emit: index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${fasta}" + def VERSION = '1.0.6' // WARN: Version information provided by tool on CLI is incorrect. Please update this string when bumping container versions. + """ + mkdir bwameme + + bwa-meme index \\ + $args \\ + -t $task.cpus \\ + -p bwameme/$prefix \\ + $fasta + + build_rmis_dna.sh bwameme/$prefix + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwameme: $VERSION + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${fasta}" + def VERSION = '1.0.6' // WARN: Version information provided by tool on CLI is incorrect. Please update this string when bumping container versions. + """ + mkdir bwameme + touch bwameme/${prefix}.0123 + touch bwameme/${prefix}.ann + touch bwameme/${prefix}.pac + touch bwameme/${prefix}.amb + touch bwameme/${prefix}.pos_packed + touch bwameme/${prefix}.suffixarray_uint64 + touch bwameme/${prefix}.suffixarray_uint64_L0_PARAMETERS + touch bwameme/${prefix}.suffixarray_uint64_L1_PARAMETERS + touch bwameme/${prefix}.suffixarray_uint64_L2_PARAMETERS + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwameme: $VERSION + END_VERSIONS + """ +} diff --git a/modules/nf-core/bwameme/index/meta.yml b/modules/nf-core/bwameme/index/meta.yml new file mode 100644 index 00000000..2db1d3a8 --- /dev/null +++ b/modules/nf-core/bwameme/index/meta.yml @@ -0,0 +1,44 @@ +name: "bwameme_index" +description: Create BWA-MEME index for reference genome +keywords: + - index + - fasta + - genome + - reference +tools: + - "bwameme": + description: "Faster BWA-MEM2 using learned-index" + homepage: https://github.com/kaist-ina/BWA-MEME + documentation: https://github.com/kaist-ina/BWA-MEME#getting-started + doi: "10.1093/bioinformatics/btac137" + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - fasta: + type: file + description: Input genome fasta file + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - index: + type: file + description: BWA-MEME genome index files + pattern: "*.{0123,amb,ann,pac,pos_packed,suffixarray_uint64,suffixarray_uint64_L0_PARAMETERS,suffixarray_uint64_L1_PARAMETERS,suffixarray_uint64_L2_PARAMETERS}" + +authors: + - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/bwameme/index/tests/main.nf.test b/modules/nf-core/bwameme/index/tests/main.nf.test new file mode 100644 index 00000000..b61f7660 --- /dev/null +++ b/modules/nf-core/bwameme/index/tests/main.nf.test @@ -0,0 +1,59 @@ +nextflow_process { + + name "Test Process BWAMEME_INDEX" + script "../main.nf" + process "BWAMEME_INDEX" + + tag "modules" + tag "modules_nfcore" + tag "bwameme" + tag "bwameme/index" + + config "./nextflow.config" + + test("BWAMEME index") { + + when { + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("BWAMEME index - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bwameme/index/tests/main.nf.test.snap b/modules/nf-core/bwameme/index/tests/main.nf.test.snap new file mode 100644 index 00000000..e608af8b --- /dev/null +++ b/modules/nf-core/bwameme/index/tests/main.nf.test.snap @@ -0,0 +1,108 @@ +{ + "BWAMEME index - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + [ + "genome.fasta.0123:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.amb:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.ann:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.pac:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.pos_packed:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64_L0_PARAMETERS:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64_L1_PARAMETERS:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64_L2_PARAMETERS:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "1": [ + "versions.yml:md5,c80b8097b8a9c022e4c1e2617771ea3d" + ], + "index": [ + [ + { + "id": "test" + }, + [ + "genome.fasta.0123:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.amb:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.ann:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.pac:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.pos_packed:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64_L0_PARAMETERS:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64_L1_PARAMETERS:md5,d41d8cd98f00b204e9800998ecf8427e", + "genome.fasta.suffixarray_uint64_L2_PARAMETERS:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "versions": [ + "versions.yml:md5,c80b8097b8a9c022e4c1e2617771ea3d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-15T13:44:38.551252246" + }, + "BWAMEME index": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + [ + "genome.fasta.0123:md5,b02870de80106104abcb03cd9463e7d8", + "genome.fasta.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.fasta.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.fasta.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", + "genome.fasta.pos_packed:md5,3534bc04a547d02d6e4cb50908a40db9", + "genome.fasta.suffixarray_uint64:md5,51abb48f687661f88bf5bf5d370521e3", + "genome.fasta.suffixarray_uint64_L0_PARAMETERS:md5,ad6397d3d33bbb6a31b1320349e23274", + "genome.fasta.suffixarray_uint64_L1_PARAMETERS:md5,6c0d6dc7e733a7f373aa7b2730621aa4", + "genome.fasta.suffixarray_uint64_L2_PARAMETERS:md5,b2d4bad4e9f0e8960a0af12b7038ab1e" + ] + ] + ], + "1": [ + "versions.yml:md5,c80b8097b8a9c022e4c1e2617771ea3d" + ], + "index": [ + [ + { + "id": "test" + }, + [ + "genome.fasta.0123:md5,b02870de80106104abcb03cd9463e7d8", + "genome.fasta.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.fasta.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.fasta.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", + "genome.fasta.pos_packed:md5,3534bc04a547d02d6e4cb50908a40db9", + "genome.fasta.suffixarray_uint64:md5,51abb48f687661f88bf5bf5d370521e3", + "genome.fasta.suffixarray_uint64_L0_PARAMETERS:md5,ad6397d3d33bbb6a31b1320349e23274", + "genome.fasta.suffixarray_uint64_L1_PARAMETERS:md5,6c0d6dc7e733a7f373aa7b2730621aa4", + "genome.fasta.suffixarray_uint64_L2_PARAMETERS:md5,b2d4bad4e9f0e8960a0af12b7038ab1e" + ] + ] + ], + "versions": [ + "versions.yml:md5,c80b8097b8a9c022e4c1e2617771ea3d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-15T13:44:25.040725565" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwameme/index/tests/nextflow.config b/modules/nf-core/bwameme/index/tests/nextflow.config new file mode 100644 index 00000000..5934b7d1 --- /dev/null +++ b/modules/nf-core/bwameme/index/tests/nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: BWAMEME_INDEX { + ext.args = '-a meme' + } + +} diff --git a/modules/nf-core/bwameme/index/tests/tags.yml b/modules/nf-core/bwameme/index/tests/tags.yml new file mode 100644 index 00000000..ddbcab04 --- /dev/null +++ b/modules/nf-core/bwameme/index/tests/tags.yml @@ -0,0 +1,2 @@ +bwameme/index: + - "modules/nf-core/bwameme/index/**" diff --git a/modules/nf-core/bwameme/mem/environment.yml b/modules/nf-core/bwameme/mem/environment.yml new file mode 100644 index 00000000..388eedd2 --- /dev/null +++ b/modules/nf-core/bwameme/mem/environment.yml @@ -0,0 +1,11 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "bwameme_mem" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::bwa-meme=1.0.6" + - "bioconda::mbuffer=20160228" + - "bioconda::samtools=1.20" diff --git a/modules/nf-core/bwameme/mem/main.nf b/modules/nf-core/bwameme/mem/main.nf new file mode 100644 index 00000000..db41316e --- /dev/null +++ b/modules/nf-core/bwameme/mem/main.nf @@ -0,0 +1,94 @@ +process BWAMEME_MEM { + tag "$meta.id" + label 'process_high' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-ed29b84fa94419f5a7bf6a841ddbcb964768825b:139b5e403886ad278b9ad139174967441c1c6ff3-0': + 'biocontainers/mulled-v2-ed29b84fa94419f5a7bf6a841ddbcb964768825b:139b5e403886ad278b9ad139174967441c1c6ff3-0' }" + + input: + tuple val(meta), path(reads) + tuple val(meta2), path(index) + tuple val(meta3), path(fasta) + val sort_bam + + output: + tuple val(meta), path("*.sam") , emit: sam , optional:true + tuple val(meta), path("*.bam") , emit: bam , optional:true + tuple val(meta), path("*.cram") , emit: cram, optional:true + tuple val(meta), path("*.crai") , emit: crai, optional:true + tuple val(meta), path("*.csi") , emit: csi , optional:true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def samtools_command = sort_bam ? 'sort' : 'view' + def mbuffer_mem = 3072 + if (!task.memory) { + log.info '[bwameme-mbuffer] Available memory not known - defaulting to 3GB for mbuffer. Specify process memory requirements to change this.' + } else { + mbuffer_mem = (task.memory.mega*0.5).intValue() + } + def mbuffer_command = sort_bam ? "| mbuffer -m ${mbuffer_mem}M" : "" + def mem_per_thread = sort_bam ? "-m "+ (mbuffer_mem/task.cpus).intValue()+"M" : "" + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension_matcher = (args2 =~ extension_pattern) + def extension = extension_matcher.getCount() > 0 ? extension_matcher[0][2].toLowerCase() : "bam" + def reference = fasta && extension=="cram" ? "--reference ${fasta}" : "" + if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output" + def VERSION = '1.0.6' // WARN: Version information provided by tool on CLI is incorrect. Please update this string when bumping container versions. + """ + INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'` + + bwa-meme \\ + mem \\ + $args \\ + -t $task.cpus \\ + \$INDEX \\ + $reads \\ + $mbuffer_command \\ + | samtools $samtools_command $args2 $mem_per_thread -@ $task.cpus ${reference} -o ${prefix}.${extension} - + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwameme: $VERSION + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def samtools_command = sort_bam ? 'sort' : 'view' + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension_matcher = (args2 =~ extension_pattern) + def extension = extension_matcher.getCount() > 0 ? extension_matcher[0][2].toLowerCase() : "bam" + def reference = fasta && extension=="cram" ? "--reference ${fasta}" : "" + if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output" + + def create_index = "" + if (extension == "cram") { + create_index = "touch ${prefix}.crai" + } else if (extension == "bam") { + create_index = "touch ${prefix}.csi" + } + def VERSION = '1.0.6' // WARN: Version information provided by tool on CLI is incorrect. Please update this string when bumping container versions. + """ + touch ${prefix}.${extension} + ${create_index} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwameme: $VERSION + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bwameme/mem/meta.yml b/modules/nf-core/bwameme/mem/meta.yml new file mode 100644 index 00000000..c7eb7b28 --- /dev/null +++ b/modules/nf-core/bwameme/mem/meta.yml @@ -0,0 +1,90 @@ +name: "bwameme_mem" +description: Performs fastq alignment to a fasta reference using BWA-MEME +keywords: + - mem + - bwa + - bwamem2 + - bwameme + - alignment + - map + - fastq + - bam + - sam + - cram +tools: + - "bwameme": + description: "Faster BWA-MEM2 using learned-index" + homepage: https://github.com/kaist-ina/BWA-MEME + documentation: https://github.com/kaist-ina/BWA-MEME#getting-started + doi: "10.1093/bioinformatics/btac137" + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - meta2: + type: map + description: | + Groovy Map containing reference/index information + e.g. [ id:'test' ] + - index: + type: file + description: BWA genome index files + pattern: "Directory containing BWA index *.{0132,amb,ann,bwt.2bit.64,pac}" + - meta3: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] + - fasta: + type: file + description: Reference genome in FASTA format + pattern: "*.{fa,fasta,fna}" + - sort_bam: + type: boolean + description: use samtools sort (true) or samtools view (false) + pattern: "true or false" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - sam: + type: file + description: Output SAM file containing read alignments + pattern: "*.{sam}" + - bam: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - cram: + type: file + description: Output CRAM file containing read alignments + pattern: "*.{cram}" + - crai: + type: file + description: Index file for CRAM file + pattern: "*.{crai}" + - csi: + type: file + description: Index file for BAM file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@ramprasadn" +maintainers: + - "@ramprasadn" diff --git a/modules/nf-core/bwameme/mem/tests/main.nf.test b/modules/nf-core/bwameme/mem/tests/main.nf.test new file mode 100644 index 00000000..3b67b39e --- /dev/null +++ b/modules/nf-core/bwameme/mem/tests/main.nf.test @@ -0,0 +1,239 @@ +nextflow_process { + + name "Test Process BWAMEME_MEM" + script "../main.nf" + process "BWAMEME_MEM" + + tag "modules" + tag "modules_nfcore" + tag "bwameme" + tag "bwameme/mem" + tag "bwameme/index" + config "./nextflow.config" + + test("sarscov2 - fastq, index, fasta, false") { + + setup { + run("BWAMEME_INDEX") { + script "../../index/main.nf" + config "./nextflow.config" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) + input[1] = BWAMEME_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, fasta, true") { + + setup { + run("BWAMEME_INDEX") { + script "../../index/main.nf" + config "./nextflow.config" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) + input[1] = BWAMEME_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, false") { + + setup { + run("BWAMEME_INDEX") { + script "../../index/main.nf" + config "./nextflow.config" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = BWAMEME_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, true") { + + setup { + run("BWAMEME_INDEX") { + script "../../index/main.nf" + config "./nextflow.config" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = BWAMEME_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, fasta, true - stub") { + + options "-stub" + + setup { + run("BWAMEME_INDEX") { + script "../../index/main.nf" + config "./nextflow.config" + process { + """ + input[0] = Channel.of([ + [:], // meta map + [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = BWAMEME_INDEX.out.index + input[2] = Channel.of([[:], [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)]]) + input[3] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } +} \ No newline at end of file diff --git a/modules/nf-core/bwameme/mem/tests/main.nf.test.snap b/modules/nf-core/bwameme/mem/tests/main.nf.test.snap new file mode 100644 index 00000000..281011ae --- /dev/null +++ b/modules/nf-core/bwameme/mem/tests/main.nf.test.snap @@ -0,0 +1,67 @@ +{ + "sarscov2 - [fastq1, fastq2], index, fasta, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,700d57071f430eb29b3c67d0a199eb95" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-15T20:04:31.962017214" + }, + "sarscov2 - [fastq1, fastq2], index, fasta, true - stub": { + "content": [ + "test.bam", + [ + "versions.yml:md5,700d57071f430eb29b3c67d0a199eb95" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-15T19:28:46.895668666" + }, + "sarscov2 - [fastq1, fastq2], index, fasta, true": { + "content": [ + "test.bam", + [ + "versions.yml:md5,700d57071f430eb29b3c67d0a199eb95" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-15T20:44:56.510177191" + }, + "sarscov2 - fastq, index, fasta, false": { + "content": [ + "test.bam", + [ + "versions.yml:md5,700d57071f430eb29b3c67d0a199eb95" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-15T20:00:05.782384898" + }, + "sarscov2 - fastq, index, fasta, true": { + "content": [ + "test.bam", + [ + "versions.yml:md5,700d57071f430eb29b3c67d0a199eb95" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-15T20:44:05.2657749" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwameme/mem/tests/nextflow.config b/modules/nf-core/bwameme/mem/tests/nextflow.config new file mode 100644 index 00000000..e7dd707e --- /dev/null +++ b/modules/nf-core/bwameme/mem/tests/nextflow.config @@ -0,0 +1,9 @@ +process { + + withName: BWAMEME_INDEX { + ext.args = '-a meme' + } + withName: BWAMEME_MEM { + ext.args = '-7' + } +} diff --git a/modules/nf-core/bwameme/mem/tests/tags.yml b/modules/nf-core/bwameme/mem/tests/tags.yml new file mode 100644 index 00000000..9e3a26cf --- /dev/null +++ b/modules/nf-core/bwameme/mem/tests/tags.yml @@ -0,0 +1,2 @@ +bwameme/mem: + - "modules/nf-core/bwameme/mem/**" From fbddb916ccf99e465ef8107ea1e2257a8f7df540 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 19 May 2024 16:31:39 +0200 Subject: [PATCH 56/80] add align --- conf/modules/align_MT.config | 10 +++++++ conf/modules/align_bwa_bwamem2.config | 7 +++++ conf/modules/prepare_references.config | 10 +++++++ main.nf | 1 + nextflow_schema.json | 10 ++++++- subworkflows/local/align.nf | 9 ++++-- subworkflows/local/alignment/align_MT.nf | 9 +++++- .../local/alignment/align_bwa_bwamem2.nf | 8 ++++- subworkflows/local/prepare_references.nf | 30 ++++++++++++++----- workflows/raredisease.nf | 5 ++++ 10 files changed, 86 insertions(+), 13 deletions(-) diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index 1bd88f14..3ed86cf5 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -23,6 +23,11 @@ process { ext.prefix = { "${meta.id}_sorted" } } + withName: '.*ALIGN_MT:BWAMEME_MEM_MT' { + ext.args = { "-7 -M -K 100000000 -R ${meta.read_group}" } + ext.prefix = { "${meta.id}_sorted" } + } + withName: '.*ALIGN_MT:BWA_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes")) } @@ -74,6 +79,11 @@ process { ext.prefix = { "${meta.id}_sorted_shifted" } } + withName: '.*ALIGN_MT_SHIFT:BWAMEME_MEM_MT' { + ext.args = { "-7 -M -K 100000000 -R ${meta.read_group}" } + ext.prefix = { "${meta.id}_sorted_shifted" } + } + withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } ext.when = { !(params.analysis_type.equals("wes"))} diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2.config index 30d14cf4..82f812da 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2.config @@ -24,6 +24,13 @@ process { ext.when = { params.aligner.equals("bwamem2") } } + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEME_MEM' { + ext.args = { "-7 -M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } + ext.prefix = { "${meta.id}_sorted" } + ext.when = { params.aligner.equals("bwameme") } + } + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.args2 = { "-T ./samtools_sort_tmp" } diff --git a/conf/modules/prepare_references.config b/conf/modules/prepare_references.config index 103a1ad7..be3602af 100644 --- a/conf/modules/prepare_references.config +++ b/conf/modules/prepare_references.config @@ -29,10 +29,20 @@ process { ext.when = {!params.bwamem2 && params.aligner == "bwamem2"} } + withName: '.*PREPARE_REFERENCES:BWAMEME_INDEX_GENOME' { + ext.args = '-a meme' + ext.when = {!params.bwameme && params.aligner == "bwameme"} + } + withName: '.*PREPARE_REFERENCES:BWAMEM2_INDEX_MT_SHIFT' { ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwamem2"} } + withName: '.*PREPARE_REFERENCES:BWAMEME_INDEX_MT_SHIFT' { + ext.args = '-a meme' + ext.when = { !(params.analysis_type == "wes") && params.aligner == "bwameme"} + } + withName: '.*PREPARE_REFERENCES:SENTIEON_BWAINDEX_GENOME' { ext.when = {!params.bwa && params.aligner == "sentieon"} } diff --git a/main.nf b/main.nf index aa92e744..838d75d7 100644 --- a/main.nf +++ b/main.nf @@ -21,6 +21,7 @@ params.fasta = getGenomeAttribute('fasta') params.fai = getGenomeAttribute('fai') params.bwa = getGenomeAttribute('bwa') params.bwamem2 = getGenomeAttribute('bwamem2') +params.bwameme = getGenomeAttribute('bwameme') params.call_interval = getGenomeAttribute('call_interval') params.cadd_resources = getGenomeAttribute('cadd_resources') params.gcnvcaller_model = getGenomeAttribute('gcnvcaller_model') diff --git a/nextflow_schema.json b/nextflow_schema.json index 8e5ed746..3b92976b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -74,6 +74,14 @@ "help_text": "If none provided, will be generated automatically from the FASTA reference.", "fa_icon": "fas fa-folder-open" }, + "bwameme": { + "type": "string", + "exists": true, + "format": "directory-path", + "description": "Directory for pre-built bwameme's learned index.", + "help_text": "If none provided, will be generated automatically from the FASTA reference.", + "fa_icon": "fas fa-folder-open" + }, "cadd_resources": { "type": "string", "exists": true, @@ -579,7 +587,7 @@ "default": "bwamem2", "description": "Specifies the alignment algorithm to use - available options are 'bwamem2' and 'sentieon'.", "fa_icon": "fas fa-align-center", - "enum": ["bwa", "bwamem2", "sentieon"] + "enum": ["bwa", "bwamem2", "bwameme", "sentieon"] }, "min_trimmed_length": { "type": "integer", diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 51bbf3fb..23df720f 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -17,9 +17,11 @@ workflow ALIGN { ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] ch_genome_bwaindex // channel: [mandatory] [ val(meta), path(index) ] ch_genome_bwamem2index // channel: [mandatory] [ val(meta), path(index) ] + ch_genome_bwamemeindex // channel: [mandatory] [ val(meta), path(index) ] ch_genome_dictionary // channel: [mandatory] [ val(meta), path(dict) ] ch_mtshift_bwaindex // channel: [mandatory] [ val(meta), path(index) ] ch_mtshift_bwamem2index // channel: [mandatory] [ val(meta), path(index) ] + ch_mtshift_bwamemeindex // channel: [mandatory] [ val(meta), path(index) ] ch_mtshift_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_mtshift_dictionary // channel: [mandatory] [ val(meta), path(dict) ] ch_mtshift_fai // channel: [mandatory] [ val(meta), path(fai) ] @@ -40,11 +42,12 @@ workflow ALIGN { ch_fastp_json = FASTP.out.json } - if (params.aligner.equals("bwamem2") || params.aligner.equals("bwa")) { - ALIGN_BWA_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 or bwa + if (params.aligner.equals("bwamem2") || params.aligner.equals("bwa") || params.aligner.equals("bwameme")) { + ALIGN_BWA_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 or bwa or bwameme ch_reads, ch_genome_bwaindex, ch_genome_bwamem2index, + ch_genome_bwamemeindex, ch_genome_fasta, ch_genome_fai, val_platform @@ -82,6 +85,7 @@ workflow ALIGN { CONVERT_MT_BAM_TO_FASTQ.out.bam, ch_genome_bwaindex, ch_genome_bwamem2index, + ch_genome_bwamemeindex, ch_genome_fasta, ch_genome_dictionary, ch_genome_fai @@ -92,6 +96,7 @@ workflow ALIGN { CONVERT_MT_BAM_TO_FASTQ.out.bam, ch_mtshift_bwaindex, ch_mtshift_bwamem2index, + ch_mtshift_bwamemeindex, ch_mtshift_fasta, ch_mtshift_dictionary, ch_mtshift_fai diff --git a/subworkflows/local/alignment/align_MT.nf b/subworkflows/local/alignment/align_MT.nf index 8f7930c7..9eb9ec43 100644 --- a/subworkflows/local/alignment/align_MT.nf +++ b/subworkflows/local/alignment/align_MT.nf @@ -5,6 +5,7 @@ include { BWA_MEM as BWA_MEM_MT } from '../../../modules/nf-core/bwa/mem/main' include { SENTIEON_BWAMEM as SENTIEON_BWAMEM_MT } from '../../../modules/nf-core/sentieon/bwamem/main' include { BWAMEM2_MEM as BWAMEM2_MEM_MT } from '../../../modules/nf-core/bwamem2/mem/main' +include { BWAMEME_MEM as BWAMEME_MEM_MT } from '../../../modules/nf-core/bwameme/mem/main' include { GATK4_MERGEBAMALIGNMENT as GATK4_MERGEBAMALIGNMENT_MT } from '../../../modules/nf-core/gatk4/mergebamalignment/main' include { PICARD_ADDORREPLACEREADGROUPS as PICARD_ADDORREPLACEREADGROUPS_MT } from '../../../modules/nf-core/picard/addorreplacereadgroups/main' include { PICARD_MARKDUPLICATES as PICARD_MARKDUPLICATES_MT } from '../../../modules/nf-core/picard/markduplicates/main' @@ -17,6 +18,7 @@ workflow ALIGN_MT { ch_ubam // channel: [mandatory] [ val(meta), path(bam) ] ch_bwaindex // channel: [mandatory for sentieon] [ val(meta), path(index) ] ch_bwamem2index // channel: [mandatory for bwamem2] [ val(meta), path(index) ] + ch_bwamemeindex // channel: [mandatory for bwameme] [ val(meta), path(index) ] ch_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_dict // channel: [mandatory] [ val(meta), path(dict) ] ch_fai // channel: [mandatory] [ val(meta), path(fai) ] @@ -26,6 +28,7 @@ workflow ALIGN_MT { ch_bwa_bam = Channel.empty() ch_bwamem2_bam = Channel.empty() ch_sentieon_bam = Channel.empty() + ch_bwameme_bam = Channel.empty() if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM_MT (ch_fastq, ch_bwamem2index, true) @@ -39,9 +42,13 @@ workflow ALIGN_MT { BWA_MEM_MT ( ch_fastq, ch_bwaindex, true ) ch_bwa_bam = BWA_MEM_MT.out.bam ch_versions = ch_versions.mix(BWA_MEM_MT.out.versions.first()) + } else if (params.aligner.equals("bwameme")) { + BWAMEME_MEM_MT (ch_fastq, ch_bwamemeindex, ch_fasta, true) + ch_bwameme_bam = BWAMEME_MEM_MT.out.bam + ch_versions = ch_versions.mix(BWAMEME_MEM_MT.out.versions.first()) } Channel.empty() - .mix(ch_bwamem2_bam, ch_sentieon_bam, ch_bwa_bam) + .mix(ch_bwamem2_bam, ch_sentieon_bam, ch_bwa_bam, ch_bwameme_bam) .join(ch_ubam, failOnMismatch:true, failOnDuplicate:true) .set {ch_bam_ubam} diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2.nf index ab5b8d9b..1670dc37 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2.nf @@ -4,6 +4,7 @@ include { BWA_MEM } from '../../../modules/nf-core/bwa/mem/main' include { BWAMEM2_MEM } from '../../../modules/nf-core/bwamem2/mem/main' +include { BWAMEME_MEM } from '../../../modules/nf-core/bwameme/mem/main' include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_ALIGN } from '../../../modules/nf-core/samtools/index/main' include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_MARKDUP } from '../../../modules/nf-core/samtools/index/main' include { SAMTOOLS_STATS } from '../../../modules/nf-core/samtools/stats/main' @@ -16,6 +17,7 @@ workflow ALIGN_BWA_BWAMEM2 { ch_reads_input // channel: [mandatory] [ val(meta), path(reads_input) ] ch_bwa_index // channel: [mandatory] [ val(meta), path(bwamem2_index) ] ch_bwamem2_index // channel: [mandatory] [ val(meta), path(bwamem2_index) ] + ch_bwameme_index // channel: [mandatory] [ val(meta), path(bwamem2_index) ] ch_genome_fasta // channel: [mandatory] [ val(meta), path(fasta) ] ch_genome_fai // channel: [mandatory] [ val(meta), path(fai) ] val_platform // string: [mandatory] default: illumina @@ -28,10 +30,14 @@ workflow ALIGN_BWA_BWAMEM2 { BWA_MEM ( ch_reads_input, ch_bwa_index, true ) ch_align = BWA_MEM.out.bam ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) - } else { + } else if (params.aligner.equals("bwamem2")) { BWAMEM2_MEM ( ch_reads_input, ch_bwamem2_index, true ) ch_align = BWAMEM2_MEM.out.bam ch_versions = ch_versions.mix(BWAMEM2_MEM.out.versions.first()) + } else { + BWAMEME_MEM ( ch_reads_input, ch_bwameme_index, ch_genome_fasta, true ) + ch_align = BWAMEME_MEM.out.bam + ch_versions = ch_versions.mix(BWAMEME_MEM.out.versions.first()) } SAMTOOLS_INDEX_ALIGN ( ch_align ) diff --git a/subworkflows/local/prepare_references.nf b/subworkflows/local/prepare_references.nf index 64d33248..2bd4b6dd 100644 --- a/subworkflows/local/prepare_references.nf +++ b/subworkflows/local/prepare_references.nf @@ -6,6 +6,8 @@ include { BWA_INDEX as BWA_INDEX_GENOME } from '../../modul include { BWA_INDEX as BWA_INDEX_MT_SHIFT } from '../../modules/nf-core/bwa/index/main' include { BWAMEM2_INDEX as BWAMEM2_INDEX_GENOME } from '../../modules/nf-core/bwamem2/index/main' include { BWAMEM2_INDEX as BWAMEM2_INDEX_MT_SHIFT } from '../../modules/nf-core/bwamem2/index/main' +include { BWAMEME_INDEX as BWAMEME_INDEX_GENOME } from '../../modules/nf-core/bwameme/index/main' +include { BWAMEME_INDEX as BWAMEME_INDEX_MT_SHIFT } from '../../modules/nf-core/bwameme/index/main' include { CAT_CAT as CAT_CAT_BAIT } from '../../modules/nf-core/cat/cat/main' include { GATK4_BEDTOINTERVALLIST as GATK_BILT } from '../../modules/nf-core/gatk4/bedtointervallist/main' include { GATK4_CREATESEQUENCEDICTIONARY as GATK_SD } from '../../modules/nf-core/gatk4/createsequencedictionary/main' @@ -45,24 +47,27 @@ workflow PREPARE_REFERENCES { ch_sentieonbwa = Channel.empty() // Genome indices - BWA_INDEX_GENOME(ch_genome_fasta).index.set{ch_bwa} - BWAMEM2_INDEX_GENOME(ch_genome_fasta) - SENTIEON_BWAINDEX_GENOME(ch_genome_fasta).index.set{ch_sentieonbwa} SAMTOOLS_FAIDX_GENOME(ch_genome_fasta, [[],[]]) GATK_SD(ch_genome_fasta) ch_fai = Channel.empty().mix(ch_genome_fai, SAMTOOLS_FAIDX_GENOME.out.fai).collect() GET_CHROM_SIZES( ch_fai ) - ch_genome_fasta.map { meta, fasta -> return [meta, fasta, [], [] ] } - .set {ch_rtgformat_in} - RTGTOOLS_FORMAT(ch_rtgformat_in) - // MT indices + // Genome alignment indices + BWA_INDEX_GENOME(ch_genome_fasta).index.set{ch_bwa} + BWAMEM2_INDEX_GENOME(ch_genome_fasta) + BWAMEME_INDEX_GENOME(ch_genome_fasta) + SENTIEON_BWAINDEX_GENOME(ch_genome_fasta).index.set{ch_sentieonbwa} + + // MT genome indices SAMTOOLS_EXTRACT_MT(ch_genome_fasta, ch_fai) ch_mt_fasta_in = Channel.empty().mix(ch_mt_fasta, SAMTOOLS_EXTRACT_MT.out.fa).collect() SAMTOOLS_FAIDX_MT_SHIFT(ch_mt_fasta_in, [[],[]]) GATK_SD_MT_SHIFT(ch_mt_fasta_in) GATK_SHIFTFASTA(ch_mt_fasta_in, SAMTOOLS_FAIDX_MT_SHIFT.out.fai, GATK_SD_MT_SHIFT.out.dict) + + // MT alignment indices BWAMEM2_INDEX_MT_SHIFT(GATK_SHIFTFASTA.out.shift_fa) + BWAMEME_INDEX_MT_SHIFT(GATK_SHIFTFASTA.out.shift_fa) BWA_INDEX_MT_SHIFT(GATK_SHIFTFASTA.out.shift_fa) SENTIEON_BWAINDEX_MT_SHIFT(GATK_SHIFTFASTA.out.shift_fa) ch_bwa_mtshift = Channel.empty().mix(SENTIEON_BWAINDEX_MT_SHIFT.out.index, BWA_INDEX_MT_SHIFT.out.index).collect() @@ -79,7 +84,6 @@ workflow PREPARE_REFERENCES { // Vcf, tab and bed indices TABIX_DBSNP(ch_known_dbsnp) - ch_versions = ch_versions.mix(TABIX_DBSNP.out.versions) TABIX_GNOMAD_AF(ch_gnomad_af_tab) TABIX_PT(ch_target_bed).tbi.set { ch_tbi } TABIX_PBT(ch_target_bed).gz_tbi.set { ch_bgzip_tbi } @@ -101,9 +105,15 @@ workflow PREPARE_REFERENCES { GATK_PREPROCESS_WGS (ch_genome_fasta, ch_fai, GATK_SD.out.dict, [[],[]], [[],[]]).set {ch_preprocwgs} GATK_PREPROCESS_WES (ch_genome_fasta, ch_fai, GATK_SD.out.dict, GATK_BILT.out.interval_list, [[],[]]).set {ch_preprocwes} + // RTG tools + ch_genome_fasta.map { meta, fasta -> return [meta, fasta, [], [] ] } + .set {ch_rtgformat_in} + RTGTOOLS_FORMAT(ch_rtgformat_in) + // Gather versions ch_versions = ch_versions.mix(BWA_INDEX_GENOME.out.versions) ch_versions = ch_versions.mix(BWAMEM2_INDEX_GENOME.out.versions) + ch_versions = ch_versions.mix(BWAMEME_INDEX_GENOME.out.versions) ch_versions = ch_versions.mix(SENTIEON_BWAINDEX_GENOME.out.versions) ch_versions = ch_versions.mix(SAMTOOLS_FAIDX_GENOME.out.versions) ch_versions = ch_versions.mix(GATK_SD.out.versions) @@ -112,12 +122,14 @@ workflow PREPARE_REFERENCES { ch_versions = ch_versions.mix(SAMTOOLS_FAIDX_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(GATK_SD_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(GATK_SHIFTFASTA.out.versions) + ch_versions = ch_versions.mix(BWAMEME_INDEX_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(BWAMEM2_INDEX_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(BWA_INDEX_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(SENTIEON_BWAINDEX_MT_SHIFT.out.versions) ch_versions = ch_versions.mix(TABIX_GNOMAD_AF.out.versions) ch_versions = ch_versions.mix(TABIX_PT.out.versions) ch_versions = ch_versions.mix(TABIX_PBT.out.versions) + ch_versions = ch_versions.mix(TABIX_DBSNP.out.versions) ch_versions = ch_versions.mix(GATK_BILT.out.versions) ch_versions = ch_versions.mix(GATK_ILT.out.versions) ch_versions = ch_versions.mix(CAT_CAT_BAIT.out.versions) @@ -129,6 +141,7 @@ workflow PREPARE_REFERENCES { emit: genome_bwa_index = Channel.empty().mix(ch_bwa, ch_sentieonbwa).collect() // channel: [ val(meta), path(index) ] genome_bwamem2_index = BWAMEM2_INDEX_GENOME.out.index.collect() // channel: [ val(meta), path(index) ] + genome_bwameme_index = BWAMEME_INDEX_GENOME.out.index.collect() // channel: [ val(meta), path(index) ] genome_chrom_sizes = GET_CHROM_SIZES.out.sizes.collect() // channel: [ path(sizes) ] genome_fai = ch_fai // channel: [ val(meta), path(fai) ] genome_dict = GATK_SD.out.dict.collect() // channel: [ path(dict) ] @@ -143,6 +156,7 @@ workflow PREPARE_REFERENCES { mtshift_dict = GATK_SHIFTFASTA.out.dict.collect() // channel: [ path(dict) ] mtshift_bwa_index = ch_bwa_mtshift // channel: [ val(meta), path(index) ] mtshift_bwamem2_index = BWAMEM2_INDEX_MT_SHIFT.out.index.collect() // channel: [ val(meta), path(index) ] + mtshift_bwameme_index = BWAMEME_INDEX_MT_SHIFT.out.index.collect() // channel: [ val(meta), path(index) ] gnomad_af_idx = TABIX_GNOMAD_AF.out.tbi.collect() // channel: [ val(meta), path(fasta) ] known_dbsnp_tbi = TABIX_DBSNP.out.tbi.collect() // channel: [ val(meta), path(fasta) ] diff --git a/workflows/raredisease.nf b/workflows/raredisease.nf index 8d3e0aa2..75e68b81 100644 --- a/workflows/raredisease.nf +++ b/workflows/raredisease.nf @@ -212,6 +212,8 @@ workflow RAREDISEASE { : ch_references.genome_bwa_index ch_genome_bwamem2index = params.bwamem2 ? Channel.fromPath(params.bwamem2).map {it -> [[id:it[0].simpleName], it]}.collect() : ch_references.genome_bwamem2_index + ch_genome_bwamemeindex = params.bwameme ? Channel.fromPath(params.bwameme).map {it -> [[id:it[0].simpleName], it]}.collect() + : ch_references.genome_bwameme_index ch_genome_chrsizes = ch_references.genome_chrom_sizes ch_genome_fai = ch_references.genome_fai ch_genome_dictionary = params.sequence_dictionary ? Channel.fromPath(params.sequence_dictionary).map {it -> [[id:it[0].simpleName], it]}.collect() @@ -242,6 +244,7 @@ workflow RAREDISEASE { ch_mtshift_backchain = ch_references.mtshift_backchain ch_mtshift_bwaindex = ch_references.mtshift_bwa_index ch_mtshift_bwamem2index = ch_references.mtshift_bwamem2_index + ch_mtshift_bwamemeindex = ch_references.mtshift_bwameme_index ch_mtshift_dictionary = ch_references.mtshift_dict ch_mtshift_fai = ch_references.mtshift_fai ch_mtshift_fasta = ch_references.mtshift_fasta @@ -355,9 +358,11 @@ workflow RAREDISEASE { ch_genome_fai, ch_genome_bwaindex, ch_genome_bwamem2index, + ch_genome_bwamemeindex, ch_genome_dictionary, ch_mtshift_bwaindex, ch_mtshift_bwamem2index, + ch_mtshift_bwamemeindex, ch_mtshift_fasta, ch_mtshift_dictionary, ch_mtshift_fai, From fdd11e008fa98ed213c3c2a3708c86e221f7c411 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Sun, 19 May 2024 16:37:22 +0200 Subject: [PATCH 57/80] update changelog --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2e74f569..aed485c1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,6 +7,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` +- A new aligner, bwameme [#553](https://github.com/nf-core/raredisease/pull/553) + ### `Changed` - Refactored config files [#538](https://github.com/nf-core/raredisease/pull/538) @@ -21,6 +23,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 | Old parameter | New parameter | | ------------- | ------------- | +| | bwameme | :::note Parameter has been updated if both old and new parameter information is present. From 4f2eb0e63dbda85ad932696773bb1f245b572e5b Mon Sep 17 00:00:00 2001 From: Sima Rahimi <54620700+sima-r@users.noreply.github.com> Date: Mon, 20 May 2024 10:40:17 +0200 Subject: [PATCH 58/80] Update CHANGELOG.md --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2e74f569..072c3d36 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,7 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` ### `Changed` - +- Changed valid values for sex according to the PED file format [#550](https://github.com/nf-core/raredisease/pull/550) - Refactored config files [#538](https://github.com/nf-core/raredisease/pull/538) - Refactored mobile element annotation subworkflow files [#538](https://github.com/nf-core/raredisease/pull/538) From 40c1e5e02601976b5fdc9aa66edc82abc1f2facf Mon Sep 17 00:00:00 2001 From: Sima Rahimi <54620700+sima-r@users.noreply.github.com> Date: Mon, 20 May 2024 11:06:39 +0200 Subject: [PATCH 59/80] ran prettier --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 072c3d36..532dcfd0 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` ### `Changed` + - Changed valid values for sex according to the PED file format [#550](https://github.com/nf-core/raredisease/pull/550) - Refactored config files [#538](https://github.com/nf-core/raredisease/pull/538) - Refactored mobile element annotation subworkflow files [#538](https://github.com/nf-core/raredisease/pull/538) From 1b98b1869a9b0603999aa17bedcbaa11f9f864de Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 20 May 2024 13:42:14 +0200 Subject: [PATCH 60/80] review suggestions --- conf/modules/align_MT.config | 8 ++++++++ ...2.config => align_bwa_bwamem2_bwameme.config} | 16 ++++++++-------- nextflow.config | 2 +- subworkflows/local/align.nf | 14 +++++++------- ...a_bwamem2.nf => align_bwa_bwamem2_bwameme.nf} | 2 +- 5 files changed, 25 insertions(+), 17 deletions(-) rename conf/modules/{align_bwa_bwamem2.config => align_bwa_bwamem2_bwameme.config} (81%) rename subworkflows/local/alignment/{align_bwa_bwamem2.nf => align_bwa_bwamem2_bwameme.nf} (99%) diff --git a/conf/modules/align_MT.config b/conf/modules/align_MT.config index bcdd1433..a616272b 100644 --- a/conf/modules/align_MT.config +++ b/conf/modules/align_MT.config @@ -19,21 +19,25 @@ process { withName: '.*ALIGN_MT:BWAMEM2_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } } withName: '.*ALIGN_MT:BWAMEME_MEM_MT' { ext.args = { "-7 -M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } } withName: '.*ALIGN_MT:BWA_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } } withName: '.*ALIGN_MT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } } @@ -72,21 +76,25 @@ process { withName: '.*ALIGN_MT_SHIFT:BWAMEM2_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted_shifted" } } withName: '.*ALIGN_MT_SHIFT:BWAMEME_MEM_MT' { ext.args = { "-7 -M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted_shifted" } } withName: '.*ALIGN_MT_SHIFT:SENTIEON_BWAMEM_MT' { ext.args = { "-M -K 10000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted_shifted" } } withName: '.*ALIGN_MT_SHIFT:BWA_MEM_MT' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } + ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted_shifted" } } diff --git a/conf/modules/align_bwa_bwamem2.config b/conf/modules/align_bwa_bwamem2_bwameme.config similarity index 81% rename from conf/modules/align_bwa_bwamem2.config rename to conf/modules/align_bwa_bwamem2_bwameme.config index 285e3c64..c217f8da 100644 --- a/conf/modules/align_bwa_bwamem2.config +++ b/conf/modules/align_bwa_bwamem2_bwameme.config @@ -17,40 +17,40 @@ process { - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM2_MEM' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:BWAMEM2_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEM_FALLBACK' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:BWAMEM_FALLBACK' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWAMEME_MEM' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:BWAMEME_MEM' { ext.args = { "-7 -M -K 100000000 -R ${meta.read_group}" } ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } ext.when = { params.aligner.equals("bwameme") } } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:BWA_MEM' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:BWA_MEM' { ext.args = { "-M -K 100000000 -R ${meta.read_group}" } ext.args2 = { "-T ./samtools_sort_tmp" } ext.prefix = { "${meta.id}_sorted" } } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_STATS' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:SAMTOOLS_STATS' { ext.args = '-s --remove-overlaps' } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_MERGE' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:SAMTOOLS_MERGE' { ext.prefix = { "${meta.id}_sorted_merged" } } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:MARKDUPLICATES' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:MARKDUPLICATES' { ext.args = "--TMP_DIR ." ext.prefix = { "${meta.id}_sorted_md" } publishDir = [ @@ -61,7 +61,7 @@ process { ] } - withName: '.*ALIGN:ALIGN_BWA_BWAMEM2:SAMTOOLS_INDEX_MARKDUP' { + withName: '.*ALIGN:ALIGN_BWA_BWAMEM2_BWAMEME:SAMTOOLS_INDEX_MARKDUP' { publishDir = [ enabled: !params.save_mapped_as_cram, path: { "${params.outdir}/alignment" }, diff --git a/nextflow.config b/nextflow.config index 63b2b529..e5f507ba 100644 --- a/nextflow.config +++ b/nextflow.config @@ -326,7 +326,7 @@ includeConfig 'conf/modules/qc_bam.config' includeConfig 'conf/modules/rank_variants.config' includeConfig 'conf/modules/scatter_genome.config' includeConfig 'conf/modules/align_MT.config' -includeConfig 'conf/modules/align_bwa_bwamem2.config' +includeConfig 'conf/modules/align_bwa_bwamem2_bwameme.config' includeConfig 'conf/modules/align_sentieon.config' includeConfig 'conf/modules/annotate_cadd.config' includeConfig 'conf/modules/call_snv_MT.config' diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 52256fb1..802bda1b 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -3,7 +3,7 @@ // include { FASTP } from '../../modules/nf-core/fastp/main' -include { ALIGN_BWA_BWAMEM2 } from './alignment/align_bwa_bwamem2' +include { ALIGN_BWA_BWAMEM2_BWAMEME } from './alignment/align_bwa_bwamem2' include { ALIGN_SENTIEON } from './alignment/align_sentieon' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' include { ALIGN_MT } from './alignment/align_MT' @@ -48,8 +48,8 @@ workflow ALIGN { ch_fastp_json = FASTP.out.json } - if (params.aligner.equals("bwamem2") || params.aligner.equals("bwa") || params.aligner.equals("bwameme")) { - ALIGN_BWA_BWAMEM2 ( // Triggered when params.aligner is set as bwamem2 or bwa or bwameme + if (params.aligner.matches("bwamem2|bwa|bwameme")) { + ALIGN_BWA_BWAMEM2_BWAMEME ( // Triggered when params.aligner is set as bwamem2 or bwa or bwameme ch_reads, ch_genome_bwaindex, ch_genome_bwamem2index, @@ -58,9 +58,9 @@ workflow ALIGN { ch_genome_fai, val_platform ) - ch_bwamem2_bam = ALIGN_BWA_BWAMEM2.out.marked_bam - ch_bwamem2_bai = ALIGN_BWA_BWAMEM2.out.marked_bai - ch_versions = ch_versions.mix(ALIGN_BWA_BWAMEM2.out.versions) + ch_bwamem2_bam = ALIGN_BWA_BWAMEM2_BWAMEME.out.marked_bam + ch_bwamem2_bai = ALIGN_BWA_BWAMEM2_BWAMEME.out.marked_bai + ch_versions = ch_versions.mix(ALIGN_BWA_BWAMEM2_BWAMEME.out.versions) } else if (params.aligner.equals("sentieon")) { ALIGN_SENTIEON ( // Triggered when params.aligner is set as sentieon ch_reads, @@ -71,7 +71,7 @@ workflow ALIGN { ) ch_sentieon_bam = ALIGN_SENTIEON.out.marked_bam ch_sentieon_bai = ALIGN_SENTIEON.out.marked_bai - ch_versions = ch_versions.mix(ALIGN_SENTIEON.out.versions) + ch_versions = ch_versions.mix(ALIGN_SENTIEON.out.versions) } ch_genome_marked_bam = Channel.empty().mix(ch_bwamem2_bam, ch_sentieon_bam) diff --git a/subworkflows/local/alignment/align_bwa_bwamem2.nf b/subworkflows/local/alignment/align_bwa_bwamem2_bwameme.nf similarity index 99% rename from subworkflows/local/alignment/align_bwa_bwamem2.nf rename to subworkflows/local/alignment/align_bwa_bwamem2_bwameme.nf index 6856ee69..87e9a958 100644 --- a/subworkflows/local/alignment/align_bwa_bwamem2.nf +++ b/subworkflows/local/alignment/align_bwa_bwamem2_bwameme.nf @@ -13,7 +13,7 @@ include { SAMTOOLS_MERGE } from '../../../modules/nf-c include { PICARD_MARKDUPLICATES as MARKDUPLICATES } from '../../../modules/nf-core/picard/markduplicates/main' -workflow ALIGN_BWA_BWAMEM2 { +workflow ALIGN_BWA_BWAMEM2_BWAMEME { take: ch_reads_input // channel: [mandatory] [ val(meta), path(reads_input) ] ch_bwa_index // channel: [mandatory] [ val(meta), path(bwamem2_index) ] From d94278a058be647381597488eb1ffc735434447f Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Mon, 20 May 2024 13:42:55 +0200 Subject: [PATCH 61/80] fix error --- subworkflows/local/align.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/align.nf b/subworkflows/local/align.nf index 802bda1b..8822d1cb 100644 --- a/subworkflows/local/align.nf +++ b/subworkflows/local/align.nf @@ -3,7 +3,7 @@ // include { FASTP } from '../../modules/nf-core/fastp/main' -include { ALIGN_BWA_BWAMEM2_BWAMEME } from './alignment/align_bwa_bwamem2' +include { ALIGN_BWA_BWAMEM2_BWAMEME } from './alignment/align_bwa_bwamem2_bwameme' include { ALIGN_SENTIEON } from './alignment/align_sentieon' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' include { ALIGN_MT } from './alignment/align_MT' From bd01ffdd606c78cc3490da9c9310d41b3b4d84b4 Mon Sep 17 00:00:00 2001 From: ramprasadn <20065894+ramprasadn@users.noreply.github.com> Date: Tue, 21 May 2024 14:16:28 +0200 Subject: [PATCH 62/80] metromap --- docs/images/raredisease_metromap_dark.pdf | Bin 199656 -> 200004 bytes docs/images/raredisease_metromap_dark.png | Bin 429662 -> 401258 bytes docs/images/raredisease_metromap_dark.svg | 2514 +++++++++--------- docs/images/raredisease_metromap_light.pdf | Bin 130783 -> 128883 bytes docs/images/raredisease_metromap_light.png | Bin 420125 -> 394146 bytes docs/images/raredisease_metromap_light.svg | 2656 ++++++++++---------- 6 files changed, 2559 insertions(+), 2611 deletions(-) diff --git a/docs/images/raredisease_metromap_dark.pdf b/docs/images/raredisease_metromap_dark.pdf index bb8df737113eb5c4f4e63c794c807cfa01f61704..030e64e00175bfae4d963f0e209e6490e4f42612 100644 GIT binary patch delta 28741 zcmZs>b8u%*)c+aVnb@{%+qUzGZGU2CV%xSgu_w;N6Whi_oA0yFZ?|e|`>$JF_jGsl z?eo5;&+D9|BMfIDB*+76{q^DrIunn4Kcnwg^MWQP`0m{sVjwYBp|3d`kVpB|%Vizn zlw_LJ?PovV;+P!Fk7zVf*B24HW42A8$bvmtNwFE}KHeDTzQU50yW5_0hX6qR&$jo| zyLO7!ZU)D6hAn&B-jtjxpR4q_g}+&ohv!3wUxtDzny-b2H3z_#=N#YeKw%wUnsjaW z`Dk0yJ7A9y6Y$mJbsyL^1$^sykAl~`7DX!jGv%Yz&SCdLX3vu1@w)xBPijODDnHuR 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