diff --git a/conf/public_aws_ecr.config b/conf/public_aws_ecr.config
deleted file mode 100644
index 93d0165f..00000000
--- a/conf/public_aws_ecr.config
+++ /dev/null
@@ -1,39 +0,0 @@
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    AWS ECR Config
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Config to set public AWS ECR images wherever possible
-    This improves speed when running on AWS infrastructure.
-    Use this as an example template when using your own private registry.
-----------------------------------------------------------------------------------------
-*/
-
-docker.registry = 'public.ecr.aws'
-podman.registry = 'public.ecr.aws'
-
-process {
-    withName: 'ADD_MOST_SEVERE_CSQ' {
-        container = 'quay.io/biocontainers/python:3.8.3'
-    }
-    withName: 'ADD_MOST_SEVERE_PLI' {
-        container = 'quay.io/biocontainers/python:3.8.3'
-    }
-    withName: 'BWA_INDEX' {
-        container = 'quay.io/biocontainers/bwa:0.7.17--hed695b0_7'
-    }
-    withName: 'FASTQC' {
-        container = 'quay.io/biocontainers/fastqc:0.11.9--0'
-    }
-    withName: 'GET_CHROM_SIZES' {
-        container = 'quay.io/biocontainers/gnu-wget:1.18--0'
-    }
-    withName: 'QUALIMAP_BAMQC' {
-        container = 'quay.io/biocontainers/qualimap:2.2.2d--1'
-    }
-    withName: 'SAMPLESHEET_CHECK' {
-        container = 'quay.io/biocontainers/python:3.8.3'
-    }
-    withName: 'UNTAR' {
-        container = 'quay.io/docker.io/ubuntu:20.04'
-    }
-}
diff --git a/nextflow.config b/nextflow.config
index ef5688fb..f891a622 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -193,9 +193,6 @@ profiles {
         executor.cpus          = 16
         executor.memory        = 60.GB
     }
-    public_aws_ecr {
-        includeConfig 'conf/public_aws_ecr.config'
-    }
     test            { includeConfig 'conf/test.config'            }
     test_full       { includeConfig 'conf/test_full.config'       }
     test_one_sample { includeConfig 'conf/test_one_sample.config' }
@@ -249,11 +246,6 @@ env {
 // Capture exit codes from upstream processes when piping
 process.shell = ['/bin/bash', '-euo', 'pipefail']
 
-// Set default registry for Docker and Podman independent of -profile
-// Will not be used unless Docker / Podman are enabled
-// Set to your registry if you have a mirror of containers
-docker.registry = 'quay.io'
-podman.registry = 'quay.io'
 
 def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
 timeline {