diff --git a/doc/api/test.md b/doc/api/test.md
index e49843f7bf7c8b..6884e128033747 100644
--- a/doc/api/test.md
+++ b/doc/api/test.md
@@ -3280,6 +3280,43 @@ test('test', (t) => {
 });
 ```
 
+#### `context.assert.fileSnapshot(value, path[, options])`
+
+<!-- YAML
+added: REPLACEME
+-->
+
+* `value` {any} A value to serialize to a string. If Node.js was started with
+  the [`--test-update-snapshots`][] flag, the serialized value is written to
+  `path`. Otherwise, the serialized value is compared to the contents of the
+  existing snapshot file.
+* `path` {string} The file where the serialized `value` is written.
+* `options` {Object} Optional configuration options. The following properties
+  are supported:
+  * `serializers` {Array} An array of synchronous functions used to serialize
+    `value` into a string. `value` is passed as the only argument to the first
+    serializer function. The return value of each serializer is passed as input
+    to the next serializer. Once all serializers have run, the resulting value
+    is coerced to a string. **Default:** If no serializers are provided, the
+    test runner's default serializers are used.
+
+This function serializes `value` and writes it to the file specified by `path`.
+
+```js
+test('snapshot test with default serialization', (t) => {
+  t.assert.fileSnapshot({ value1: 1, value2: 2 }, './snapshots/snapshot.json');
+});
+```
+
+This function differs from `context.assert.snapshot()` in the following ways:
+
+* The snapshot file path is explicitly provided by the user.
+* Each snapshot file is limited to a single snapshot value.
+* No additional escaping is performed by the test runner.
+
+These differences allow snapshot files to better support features such as syntax
+highlighting.
+
 #### `context.assert.snapshot(value[, options])`
 
 <!-- YAML
diff --git a/lib/internal/test_runner/snapshot.js b/lib/internal/test_runner/snapshot.js
index e6fcd71552c939..642e84b2f13b33 100644
--- a/lib/internal/test_runner/snapshot.js
+++ b/lib/internal/test_runner/snapshot.js
@@ -23,6 +23,7 @@ const {
   validateArray,
   validateFunction,
   validateObject,
+  validateString,
 } = require('internal/validators');
 const { strictEqual } = require('assert');
 const { mkdirSync, readFileSync, writeFileSync } = require('fs');
@@ -109,16 +110,7 @@ class SnapshotFile {
       }
       this.loaded = true;
     } catch (err) {
-      let msg = `Cannot read snapshot file '${this.snapshotFile}.'`;
-
-      if (err?.code === 'ENOENT') {
-        msg += ` ${kMissingSnapshotTip}`;
-      }
-
-      const error = new ERR_INVALID_STATE(msg);
-      error.cause = err;
-      error.filename = this.snapshotFile;
-      throw error;
+      throwReadError(err, this.snapshotFile);
     }
   }
 
@@ -132,11 +124,7 @@ class SnapshotFile {
       mkdirSync(dirname(this.snapshotFile), { __proto__: null, recursive: true });
       writeFileSync(this.snapshotFile, output, 'utf8');
     } catch (err) {
-      const msg = `Cannot write snapshot file '${this.snapshotFile}.'`;
-      const error = new ERR_INVALID_STATE(msg);
-      error.cause = err;
-      error.filename = this.snapshotFile;
-      throw error;
+      throwWriteError(err, this.snapshotFile);
     }
   }
 }
@@ -171,21 +159,18 @@ class SnapshotManager {
 
   serialize(input, serializers = serializerFns) {
     try {
-      let value = input;
-
-      for (let i = 0; i < serializers.length; ++i) {
-        const fn = serializers[i];
-        value = fn(value);
-      }
-
+      const value = serializeValue(input, serializers);
       return `\n${templateEscape(value)}\n`;
     } catch (err) {
-      const error = new ERR_INVALID_STATE(
-        'The provided serializers did not generate a string.',
-      );
-      error.input = input;
-      error.cause = err;
-      throw error;
+      throwSerializationError(input, err);
+    }
+  }
+
+  serializeWithoutEscape(input, serializers = serializerFns) {
+    try {
+      return serializeValue(input, serializers);
+    } catch (err) {
+      throwSerializationError(input, err);
     }
   }
 
@@ -222,6 +207,80 @@ class SnapshotManager {
       }
     };
   }
+
+  createFileAssert() {
+    const manager = this;
+
+    return function fileSnapshotAssertion(actual, path, options = kEmptyObject) {
+      validateString(path, 'path');
+      validateObject(options, 'options');
+      const {
+        serializers = serializerFns,
+      } = options;
+      validateFunctionArray(serializers, 'options.serializers');
+      const value = manager.serializeWithoutEscape(actual, serializers);
+
+      if (manager.updateSnapshots) {
+        try {
+          mkdirSync(dirname(path), { __proto__: null, recursive: true });
+          writeFileSync(path, value, 'utf8');
+        } catch (err) {
+          throwWriteError(err, path);
+        }
+      } else {
+        let expected;
+
+        try {
+          expected = readFileSync(path, 'utf8');
+        } catch (err) {
+          throwReadError(err, path);
+        }
+
+        strictEqual(value, expected);
+      }
+    };
+  }
+}
+
+function throwReadError(err, filename) {
+  let msg = `Cannot read snapshot file '${filename}.'`;
+
+  if (err?.code === 'ENOENT') {
+    msg += ` ${kMissingSnapshotTip}`;
+  }
+
+  const error = new ERR_INVALID_STATE(msg);
+  error.cause = err;
+  error.filename = filename;
+  throw error;
+}
+
+function throwWriteError(err, filename) {
+  const msg = `Cannot write snapshot file '${filename}.'`;
+  const error = new ERR_INVALID_STATE(msg);
+  error.cause = err;
+  error.filename = filename;
+  throw error;
+}
+
+function throwSerializationError(input, err) {
+  const error = new ERR_INVALID_STATE(
+    'The provided serializers did not generate a string.',
+  );
+  error.input = input;
+  error.cause = err;
+  throw error;
+}
+
+function serializeValue(value, serializers) {
+  let v = value;
+
+  for (let i = 0; i < serializers.length; ++i) {
+    const fn = serializers[i];
+    v = fn(v);
+  }
+
+  return v;
 }
 
 function validateFunctionArray(fns, name) {
diff --git a/lib/internal/test_runner/test.js b/lib/internal/test_runner/test.js
index fdf875ceae81b7..0dc61255874a04 100644
--- a/lib/internal/test_runner/test.js
+++ b/lib/internal/test_runner/test.js
@@ -101,14 +101,18 @@ function lazyFindSourceMap(file) {
 function lazyAssertObject(harness) {
   if (assertObj === undefined) {
     const { getAssertionMap } = require('internal/test_runner/assert');
+    const { SnapshotManager } = require('internal/test_runner/snapshot');
 
     assertObj = getAssertionMap();
-    if (!assertObj.has('snapshot')) {
-      const { SnapshotManager } = require('internal/test_runner/snapshot');
+    harness.snapshotManager = new SnapshotManager(harness.config.updateSnapshots);
 
-      harness.snapshotManager = new SnapshotManager(harness.config.updateSnapshots);
+    if (!assertObj.has('snapshot')) {
       assertObj.set('snapshot', harness.snapshotManager.createAssert());
     }
+
+    if (!assertObj.has('fileSnapshot')) {
+      assertObj.set('fileSnapshot', harness.snapshotManager.createFileAssert());
+    }
   }
   return assertObj;
 }
diff --git a/test/fixtures/test-runner/snapshots/file-snapshots.js b/test/fixtures/test-runner/snapshots/file-snapshots.js
new file mode 100644
index 00000000000000..cb085f866cfceb
--- /dev/null
+++ b/test/fixtures/test-runner/snapshots/file-snapshots.js
@@ -0,0 +1,21 @@
+'use strict';
+const { test } = require('node:test');
+
+test('snapshot file path is created', (t) => {
+  t.assert.fileSnapshot({ baz: 9 }, './foo/bar/baz/1.json');
+});
+
+test('test with plan', (t) => {
+  t.plan(2);
+  t.assert.fileSnapshot({ foo: 1, bar: 2 }, '2.json');
+  t.assert.fileSnapshot(5, '3.txt');
+});
+
+test('custom serializers are supported', (t) => {
+  t.assert.fileSnapshot({ foo: 1 }, '4.txt', {
+    serializers: [
+      (value) => { return value + '424242'; },
+      (value) => { return JSON.stringify(value); },
+    ]
+  });
+});
diff --git a/test/parallel/test-runner-assert.js b/test/parallel/test-runner-assert.js
index 73d3b107251b50..74384947278e4e 100644
--- a/test/parallel/test-runner-assert.js
+++ b/test/parallel/test-runner-assert.js
@@ -10,7 +10,7 @@ test('expected methods are on t.assert', (t) => {
     'strict',
   ];
   const assertKeys = Object.keys(assert).filter((key) => !uncopiedKeys.includes(key));
-  const expectedKeys = ['snapshot'].concat(assertKeys).sort();
+  const expectedKeys = ['snapshot', 'fileSnapshot'].concat(assertKeys).sort();
   assert.deepStrictEqual(Object.keys(t.assert).sort(), expectedKeys);
 });
 
diff --git a/test/parallel/test-runner-snapshot-file-tests.js b/test/parallel/test-runner-snapshot-file-tests.js
new file mode 100644
index 00000000000000..38984956695b82
--- /dev/null
+++ b/test/parallel/test-runner-snapshot-file-tests.js
@@ -0,0 +1,82 @@
+'use strict';
+const common = require('../common');
+const fixtures = require('../common/fixtures');
+const tmpdir = require('../common/tmpdir');
+const { suite, test } = require('node:test');
+
+tmpdir.refresh();
+
+suite('t.assert.fileSnapshot() validation', () => {
+  test('path must be a string', (t) => {
+    t.assert.throws(() => {
+      t.assert.fileSnapshot({}, 5);
+    }, /The "path" argument must be of type string/);
+  });
+
+  test('options must be an object', (t) => {
+    t.assert.throws(() => {
+      t.assert.fileSnapshot({}, '', null);
+    }, /The "options" argument must be of type object/);
+  });
+
+  test('options.serializers must be an array if present', (t) => {
+    t.assert.throws(() => {
+      t.assert.fileSnapshot({}, '', { serializers: 5 });
+    }, /The "options\.serializers" property must be an instance of Array/);
+  });
+
+  test('options.serializers must only contain functions', (t) => {
+    t.assert.throws(() => {
+      t.assert.fileSnapshot({}, '', { serializers: [() => {}, ''] });
+    }, /The "options\.serializers\[1\]" property must be of type function/);
+  });
+});
+
+suite('t.assert.fileSnapshot() update/read flow', () => {
+  const fixture = fixtures.path(
+    'test-runner', 'snapshots', 'file-snapshots.js'
+  );
+
+  test('fails prior to snapshot generation', async (t) => {
+    const child = await common.spawnPromisified(
+      process.execPath,
+      [fixture],
+      { cwd: tmpdir.path },
+    );
+
+    t.assert.strictEqual(child.code, 1);
+    t.assert.strictEqual(child.signal, null);
+    t.assert.match(child.stdout, /tests 3/);
+    t.assert.match(child.stdout, /pass 0/);
+    t.assert.match(child.stdout, /fail 3/);
+    t.assert.match(child.stdout, /Missing snapshots can be generated/);
+  });
+
+  test('passes when regenerating snapshots', async (t) => {
+    const child = await common.spawnPromisified(
+      process.execPath,
+      ['--test-update-snapshots', fixture],
+      { cwd: tmpdir.path },
+    );
+
+    t.assert.strictEqual(child.code, 0);
+    t.assert.strictEqual(child.signal, null);
+    t.assert.match(child.stdout, /tests 3/);
+    t.assert.match(child.stdout, /pass 3/);
+    t.assert.match(child.stdout, /fail 0/);
+  });
+
+  test('passes when snapshots exist', async (t) => {
+    const child = await common.spawnPromisified(
+      process.execPath,
+      [fixture],
+      { cwd: tmpdir.path },
+    );
+
+    t.assert.strictEqual(child.code, 0);
+    t.assert.strictEqual(child.signal, null);
+    t.assert.match(child.stdout, /tests 3/);
+    t.assert.match(child.stdout, /pass 3/);
+    t.assert.match(child.stdout, /fail 0/);
+  });
+});