diff --git a/package-lock.json b/package-lock.json
index f50df50692..18f942de96 100644
--- a/package-lock.json
+++ b/package-lock.json
@@ -26284,11 +26284,10 @@
       }
     },
     "node_modules/mysql2": {
-      "version": "3.11.3",
-      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.3.tgz",
-      "integrity": "sha512-Qpu2ADfbKzyLdwC/5d4W7+5Yz7yBzCU05YWt5npWzACST37wJsB23wgOSo00qi043urkiRwXtEvJc9UnuLX/MQ==",
+      "version": "3.11.5",
+      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.5.tgz",
+      "integrity": "sha512-0XFu8rUmFN9vC0ME36iBvCUObftiMHItrYFhlCRvFWbLgpNqtC4Br/NmZX1HNCszxT0GGy5QtP+k3Q3eCJPaYA==",
       "dev": true,
-      "license": "MIT",
       "dependencies": {
         "aws-ssl-profiles": "^1.1.1",
         "denque": "^2.1.0",
@@ -37853,7 +37852,7 @@
         "@types/mocha": "7.0.2",
         "@types/node": "18.18.14",
         "@types/semver": "7.5.8",
-        "mysql2": "3.11.3",
+        "mysql2": "3.11.5",
         "nyc": "15.1.0",
         "rimraf": "5.0.10",
         "semver": "7.6.3",
@@ -48554,7 +48553,7 @@
         "@types/mocha": "7.0.2",
         "@types/node": "18.18.14",
         "@types/semver": "7.5.8",
-        "mysql2": "3.11.3",
+        "mysql2": "3.11.5",
         "nyc": "15.1.0",
         "rimraf": "5.0.10",
         "semver": "7.6.3",
@@ -63019,9 +63018,9 @@
       }
     },
     "mysql2": {
-      "version": "3.11.3",
-      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.3.tgz",
-      "integrity": "sha512-Qpu2ADfbKzyLdwC/5d4W7+5Yz7yBzCU05YWt5npWzACST37wJsB23wgOSo00qi043urkiRwXtEvJc9UnuLX/MQ==",
+      "version": "3.11.5",
+      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.5.tgz",
+      "integrity": "sha512-0XFu8rUmFN9vC0ME36iBvCUObftiMHItrYFhlCRvFWbLgpNqtC4Br/NmZX1HNCszxT0GGy5QtP+k3Q3eCJPaYA==",
       "dev": true,
       "requires": {
         "aws-ssl-profiles": "^1.1.1",
diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/package.json b/plugins/node/opentelemetry-instrumentation-mysql2/package.json
index dcf3806e56..20de228c25 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/package.json
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/package.json
@@ -50,7 +50,7 @@
     "@types/mocha": "7.0.2",
     "@types/node": "18.18.14",
     "@types/semver": "7.5.8",
-    "mysql2": "3.11.3",
+    "mysql2": "3.11.5",
     "nyc": "15.1.0",
     "rimraf": "5.0.10",
     "semver": "7.6.3",
diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts b/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
index 7557cf7a01..60983abeb1 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
@@ -55,8 +55,9 @@ export class MySQL2Instrumentation extends InstrumentationBase<MySQL2Instrumenta
         'mysql2',
         ['>=1.4.2 <4'],
         (moduleExports: any) => {
-          const ConnectionPrototype: mysqlTypes.Connection =
-            moduleExports.Connection.prototype;
+          const ConnectionPrototype =
+            this._getConnectionPrototype(moduleExports);
+
           if (isWrapped(ConnectionPrototype.query)) {
             this._unwrap(ConnectionPrototype, 'query');
           }
@@ -79,8 +80,8 @@ export class MySQL2Instrumentation extends InstrumentationBase<MySQL2Instrumenta
         },
         (moduleExports: any) => {
           if (moduleExports === undefined) return;
-          const ConnectionPrototype: mysqlTypes.Connection =
-            moduleExports.Connection.prototype;
+          const ConnectionPrototype =
+            this._getConnectionPrototype(moduleExports);
           this._unwrap(ConnectionPrototype, 'query');
           this._unwrap(ConnectionPrototype, 'execute');
         }
@@ -210,4 +211,16 @@ export class MySQL2Instrumentation extends InstrumentationBase<MySQL2Instrumenta
       };
     };
   }
+
+  private _getConnectionPrototype(moduleExports: any): mysqlTypes.Connection {
+    const baseClass = Object.getPrototypeOf(moduleExports.Connection);
+
+    // return the base class prototype if it has the method
+    // we need to patch (mysql2@3.11.5+)
+    if (typeof baseClass.prototype?.query === 'function') {
+      return baseClass.prototype;
+    }
+
+    return moduleExports.Connection.prototype;
+  }
 }