diff --git a/NEWS b/NEWS
index 66896a7328fd7..c58928054b831 100644
--- a/NEWS
+++ b/NEWS
@@ -28,6 +28,9 @@ PHP                                                                        NEWS
   . Fixed bug GH-16454 (Unhandled INF in date_sunset() with tiny $utcOffset).
     (cmb)
 
+- DBA:
+  . Fixed bug GH-16390 (dba_open() can segfault for "pathless" streams). (cmb)
+
 - DOM:
   . Fixed bug GH-16316 (DOMXPath breaks when not initialized properly).
     (nielsdos)
diff --git a/ext/dba/dba.c b/ext/dba/dba.c
index 503c41be8db7c..f094fb1f612b1 100644
--- a/ext/dba/dba.c
+++ b/ext/dba/dba.c
@@ -844,10 +844,13 @@ static void php_dba_open(INTERNAL_FUNCTION_PARAMETERS, bool persistent)
 			connection->info->lock.fp = php_stream_open_wrapper(lock_name, lock_file_mode, STREAM_MUST_SEEK|REPORT_ERRORS|IGNORE_PATH|persistent_flag, &opened_path);
 			if (connection->info->lock.fp) {
 				if (is_db_lock) {
-					ZEND_ASSERT(opened_path);
-					/* replace the path info with the real path of the opened file */
-					zend_string_release_ex(connection->info->path, persistent);
-					connection->info->path = php_dba_zend_string_dup_safe(opened_path, persistent);
+					if (opened_path) {
+						/* replace the path info with the real path of the opened file */
+						zend_string_release_ex(connection->info->path, persistent);
+						connection->info->path = php_dba_zend_string_dup_safe(opened_path, persistent);
+					} else {
+						error = "Unable to determine path for locking";
+					}
 				}
 			}
 			if (opened_path) {
@@ -864,10 +867,10 @@ static void php_dba_open(INTERNAL_FUNCTION_PARAMETERS, bool persistent)
 			zval_ptr_dtor(return_value);
 			RETURN_FALSE;
 		}
-		if (!php_stream_supports_lock(connection->info->lock.fp)) {
+		if (!error && !php_stream_supports_lock(connection->info->lock.fp)) {
 			error = "Stream does not support locking";
 		}
-		if (php_stream_lock(connection->info->lock.fp, lock_mode)) {
+		if (!error && php_stream_lock(connection->info->lock.fp, lock_mode)) {
 			error = "Unable to establish lock"; /* force failure exit */
 		}
 	}
diff --git a/ext/dba/tests/gh16390.phpt b/ext/dba/tests/gh16390.phpt
new file mode 100644
index 0000000000000..0b66e742a40ab
--- /dev/null
+++ b/ext/dba/tests/gh16390.phpt
@@ -0,0 +1,11 @@
+--TEST--
+GH-16390 (dba_open() can segfault for "pathless" streams)
+--EXTENSIONS--
+dba
+--FILE--
+<?php
+$file = 'data:text/plain;z=y;uri=eviluri;mediatype=wut?;mediatype2=hello,somedata';
+$db = dba_open($file, 'c', 'inifile');
+?>
+--EXPECTF--
+Warning: dba_open(): Driver initialization failed for handler: inifile: Unable to determine path for locking in %s on line %d