Issue with CD8 T cells annotated as CD4 T cells by Azimuth

[skurkalang]

We used Azimuth to annotate the T cell subtypes, most cells were annotated as CD4 T cells but when check with CD8A, CD8B and CD4 expression, majority of them were CD8 T cells.

We then manually subset the CD8 and CD4 T cells based on CD8A, CD8B and CD4 and re run the Azimuth on the CD8 T cells , most CD8 T cells were still classified as CD4 T cells.

How to solve this issue?

Thank you in advance