From ce47f4834ce07221db3c5c382c79e635c9259813 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 6 Oct 2023 12:02:55 +0200 Subject: [PATCH 01/38] adding base version --- modules.json | 10 +++++ modules/nf-core/samtools/depth/main.nf | 39 ++++++++++++++++++ modules/nf-core/samtools/depth/meta.yml | 54 +++++++++++++++++++++++++ modules/nf-core/sexdeterrmine/main.nf | 40 ++++++++++++++++++ modules/nf-core/sexdeterrmine/meta.yml | 48 ++++++++++++++++++++++ subworkflows/local/sex_determination.nf | 37 +++++++++++++++++ 6 files changed, 228 insertions(+) create mode 100644 modules/nf-core/samtools/depth/main.nf create mode 100644 modules/nf-core/samtools/depth/meta.yml create mode 100644 modules/nf-core/sexdeterrmine/main.nf create mode 100644 modules/nf-core/sexdeterrmine/meta.yml create mode 100644 subworkflows/local/sex_determination.nf diff --git a/modules.json b/modules.json index 25e1ecc9a..24587d8da 100644 --- a/modules.json +++ b/modules.json @@ -165,6 +165,11 @@ "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, + "samtools/depth": { + "branch": "master", + "git_sha": "a1ffbc1fd87bd5a829e956cc26ec9cc53af3e817", + "installed_by": ["modules"] + }, "samtools/faidx": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", @@ -204,6 +209,11 @@ "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules", "bam_split_by_region"] + }, + "sexdeterrmine": { + "branch": "master", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": ["modules"] } } }, diff --git a/modules/nf-core/samtools/depth/main.nf b/modules/nf-core/samtools/depth/main.nf new file mode 100644 index 000000000..64b0e3a53 --- /dev/null +++ b/modules/nf-core/samtools/depth/main.nf @@ -0,0 +1,39 @@ +process SAMTOOLS_DEPTH { + tag "$meta1.id" + label 'process_low' + + conda "bioconda::samtools=1.17" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" + + input: + tuple val(meta1), path(bam) + tuple val(meta2), path(intervals) + + output: + tuple val(meta1), path("*.tsv"), emit: tsv + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta1.id}" + def positions = intervals ? "-b ${intervals}" : "" + """ + samtools \\ + depth \\ + --threads ${task.cpus-1} \\ + $args \\ + $positions \\ + -o ${prefix}.tsv \\ + $bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/depth/meta.yml b/modules/nf-core/samtools/depth/meta.yml new file mode 100644 index 000000000..f774c0f2c --- /dev/null +++ b/modules/nf-core/samtools/depth/meta.yml @@ -0,0 +1,54 @@ +name: samtools_depth +description: Computes the depth at each position or region. +keywords: + - depth + - samtools + - statistics + - coverage +tools: + - samtools: + description: Tools for dealing with SAM, BAM and CRAM files; samtools depth – computes the read depth at each position or region + homepage: http://www.htslib.org + documentation: http://www.htslib.org/doc/samtools-depth.html + tool_dev_url: https://github.com/samtools/samtools + doi: "10.1093/bioinformatics/btp352" + licence: ["MIT"] + +input: + - meta1: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: sorted BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - meta2: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test' ] + - intervals: + type: file + description: list of positions or regions in specified bed file + pattern: "*.{bed}" + +output: + - meta1: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - tsv: + type: file + description: The output of samtools depth has three columns - the name of the contig or chromosome, the position and the number of reads aligned at that position + pattern: "*.{tsv}" + +authors: + - "@louperelo" + - "@nevinwu" diff --git a/modules/nf-core/sexdeterrmine/main.nf b/modules/nf-core/sexdeterrmine/main.nf new file mode 100644 index 000000000..3f3ef2b79 --- /dev/null +++ b/modules/nf-core/sexdeterrmine/main.nf @@ -0,0 +1,40 @@ +process SEXDETERRMINE { + tag "$meta.id" + label 'process_single' + + conda "bioconda::sexdeterrmine=1.1.2" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/sexdeterrmine:1.1.2--hdfd78af_1': + 'biocontainers/sexdeterrmine:1.1.2--hdfd78af_1' }" + + input: + tuple val(meta), path(depth) + path sample_list_file + + output: + tuple val(meta), path("*.json"), emit: json + tuple val(meta), path("*.tsv") , emit: tsv + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def sample_list = sample_list_file ? '-f ${sample_list_file}' : '' + if ("$depth" == "${prefix}.tsv") error "Input depth and output TSV names are the same, set prefix in module configuration to disambiguate!" + + """ + sexdeterrmine \\ + -I $depth \\ + $sample_list \\ + $args \\ + > ${prefix}.tsv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sexdeterrmine: \$(echo \$(sexdeterrmine --version 2>&1)) + END_VERSIONS + """ +} diff --git a/modules/nf-core/sexdeterrmine/meta.yml b/modules/nf-core/sexdeterrmine/meta.yml new file mode 100644 index 000000000..b2b74eff4 --- /dev/null +++ b/modules/nf-core/sexdeterrmine/meta.yml @@ -0,0 +1,48 @@ +name: "sexdeterrmine" +description: Calculate the relative coverage on the Gonosomes vs Autosomes from the output of samtools depth, with error bars. +keywords: + - sex determination + - genetic sex + - relative coverage + - ancient dna +tools: + - "sexdeterrmine": + description: "A python script carry out calculate the relative coverage of X and Y chromosomes, and their associated error bars, out of capture data." + homepage: "https://github.com/TCLamnidis/Sex.DetERRmine" + documentation: "https://github.com/TCLamnidis/Sex.DetERRmine/README.md" + tool_dev_url: "https://github.com/TCLamnidis/Sex.DetERRmine" + doi: "10.1038/s41467-018-07483-5" + licence: "['GPL v3']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - depth: + type: file + description: Output from samtools depth (with header) + pattern: "*" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - json: + type: file + description: JSON formatted table of relative coverages on the X and Y, with associated error bars. + pattern: "*.json" + - tsv: + type: file + description: TSV table of relative coverages on the X and Y, with associated error bars. + pattern: "*.tsv" + +authors: + - "@TCLamnidis" diff --git a/subworkflows/local/sex_determination.nf b/subworkflows/local/sex_determination.nf new file mode 100644 index 000000000..8cad217c5 --- /dev/null +++ b/subworkflows/local/sex_determination.nf @@ -0,0 +1,37 @@ +// +// Run sex determine +// + +include { SAMTOOLS_DEPTH } from '../modules/nf-core/samtools/depth/main' +include { SEXDETERRMINE } from '../modules/nf-core/sexdeterrmine/main' + +workflow RUN_SEXDETERMINE { + + take: + samtoolsdepth_input // channel: [ val(meta1), [ bam ], [ meta2 ] [ intervals_bed ] ] + + main: + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() + + if ( params.run_sexdeterrmine ) { + + // Generate sex_determine input + + SAMTOOLS_DEPTH(samtoolsdepth_input).out.tsv + ch_sex_determine_input = SAMTOOLS_DEPTH.out.tsv + ch_multiqc_files = ch_multiqc_files.mix( SAMTOOLS_DEPTH.out.mqc ) + ch_versions = ch_versions.mix( SAMTOOLS_DEPTH.out.versions ) + + // Run sex determination with samtools depth input + SEXDETERRMINE(ch_sex_determine_input) + ch_coverages = SEXDETERRMINE.out.tsv + ch_multiqc_files = ch_multiqc_files.mix( SEXDETERRMINE.out.json ) + ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) + } + + emit: + covergages = ch_coverages + mqc = ch_multiqc_files + versions = ch_versions +} From 4dd74a35a9831551b8ebb1d6ec7b94c2392f262d Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 20 Oct 2023 11:52:16 +0200 Subject: [PATCH 02/38] first draft of sex determination subworkflow --- subworkflows/local/sex_determination.nf | 25 ++++++++++++++++++------- 1 file changed, 18 insertions(+), 7 deletions(-) diff --git a/subworkflows/local/sex_determination.nf b/subworkflows/local/sex_determination.nf index 8cad217c5..e3ff624a9 100644 --- a/subworkflows/local/sex_determination.nf +++ b/subworkflows/local/sex_determination.nf @@ -8,7 +8,9 @@ include { SEXDETERRMINE } from '../modules/nf-core/sexdeterrmine/main' workflow RUN_SEXDETERMINE { take: - samtoolsdepth_input // channel: [ val(meta1), [ bam ], [ meta2 ] [ intervals_bed ] ] + // samtoolsdepth_input // channel: [ val(meta1), [ bam ], [ meta2 ] [ intervals_bed ] ] + sexdeterrmine_bam // channel: [ val(meta1), [ bam ] ] + sexdeterrmine_bed // channel: [ [ meta2 ] [ intervals_bed ] ] main: ch_versions = Channel.empty() @@ -17,21 +19,30 @@ workflow RUN_SEXDETERMINE { if ( params.run_sexdeterrmine ) { // Generate sex_determine input + // Break here the input not in the workflow - SAMTOOLS_DEPTH(samtoolsdepth_input).out.tsv + ch_samtoolsdepth_input = sexdeterrmine_bam + .combine(sexdeterrmine_bed) + .multiMap{ + meta, bam, meta2, bed -> + bam = [ meta, bam ] + intervals = [ meta2, bed ] + } + + SAMTOOLS_DEPTH(ch_samtoolsdepth_input) ch_sex_determine_input = SAMTOOLS_DEPTH.out.tsv - ch_multiqc_files = ch_multiqc_files.mix( SAMTOOLS_DEPTH.out.mqc ) ch_versions = ch_versions.mix( SAMTOOLS_DEPTH.out.versions ) // Run sex determination with samtools depth input + // Does it need a sample list file to run? SEXDETERRMINE(ch_sex_determine_input) - ch_coverages = SEXDETERRMINE.out.tsv + ch_coverages = SEXDETERRMINE.out.tsv ch_multiqc_files = ch_multiqc_files.mix( SEXDETERRMINE.out.json ) ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) } emit: - covergages = ch_coverages - mqc = ch_multiqc_files - versions = ch_versions + covergages = ch_coverages // channel: [ val(meta), path("*.tsv") ] + mqc = ch_multiqc_files // channel: [ val(meta), path("*.json") ] + versions = ch_versions // channel: path(versions.yml) } From 14c132502bca4229077f59cb059a6dbfb254ca0c Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 3 Nov 2023 11:31:13 +0100 Subject: [PATCH 03/38] missing adding parameters --- conf/modules.config | 24 ++++++++++++++ conf/test_humanbam.config | 3 +- nextflow.config | 6 ++++ subworkflows/local/sex_determination.nf | 43 ++++++++++++------------- workflows/eager.nf | 40 +++++++++++++++++++++++ 5 files changed, 93 insertions(+), 23 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 904e63981..19c6b8423 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -845,4 +845,28 @@ process { enabled: true ] } + + // + // SEXDETERRMINE + // + withName: SAMTOOLS_DEPTH { + tag = { "${meta.reference}|${meta.sample_id}_${meta.library_id}" } + ext.prefix = { "${meta.sample_id}_${meta.library_id}_samtoolsdepth" } + publishDir = [ + path: { "${params.outdir}/sex_determine/samtools_depth/" }, + mode: params.publish_dir_mode, + enabled: true + ] + } + + withName: SEXDETERRMINE { + tag = { "${meta.reference}|${meta.sample_id}_${meta.library_id}" } + ext.prefix = { "${meta.sample_id}_${meta.library_id}_sexdetermine" } + publishDir = [ + path: { "${params.outdir}/sex_determine/sexdeterrmine/" }, + mode: params.publish_dir_mode, + enabled: true + ] + } + } diff --git a/conf/test_humanbam.config b/conf/test_humanbam.config index 8439f86f2..c6f120efb 100644 --- a/conf/test_humanbam.config +++ b/conf/test_humanbam.config @@ -33,7 +33,8 @@ params { // TODO Reactivate sexDet and genotyping params when those steps get implemented. // //Sex Determination - // sexdeterrmine_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' + //sexdsexdeterrmine_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' + sexdeterrmine_bedfile = '../1240K.pos.list_hs37d5.0based.bed.gz' // // Genotyping // pileupcaller_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' // pileupcaller_snpfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K_covered_in_JK2067_downsampled_s0.1.numeric_chromosomes.snp' diff --git a/nextflow.config b/nextflow.config index a20ae5a5e..a848ae244 100644 --- a/nextflow.config +++ b/nextflow.config @@ -196,6 +196,12 @@ params { // Genotyping genotyping_source = 'raw' + + // Sex determine + run_sexdeterrmine = true + sexdeterrmine_bedfile = null + sexdeterrmine_samplelist = null + } // Load base.config by default for all pipelines diff --git a/subworkflows/local/sex_determination.nf b/subworkflows/local/sex_determination.nf index e3ff624a9..7aaf6ba63 100644 --- a/subworkflows/local/sex_determination.nf +++ b/subworkflows/local/sex_determination.nf @@ -2,8 +2,8 @@ // Run sex determine // -include { SAMTOOLS_DEPTH } from '../modules/nf-core/samtools/depth/main' -include { SEXDETERRMINE } from '../modules/nf-core/sexdeterrmine/main' +include { SAMTOOLS_DEPTH } from '../../modules/nf-core/samtools/depth/main' +include { SEXDETERRMINE } from '../../modules/nf-core/sexdeterrmine/main' workflow RUN_SEXDETERMINE { @@ -11,38 +11,37 @@ workflow RUN_SEXDETERMINE { // samtoolsdepth_input // channel: [ val(meta1), [ bam ], [ meta2 ] [ intervals_bed ] ] sexdeterrmine_bam // channel: [ val(meta1), [ bam ] ] sexdeterrmine_bed // channel: [ [ meta2 ] [ intervals_bed ] ] + sexdeterrmine_list // channel: [ val(meta), [ sample_list_file ] ] main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - if ( params.run_sexdeterrmine ) { - // Generate sex_determine input - // Break here the input not in the workflow + // Generate sex_determine input + // Break here the input not in the workflow - ch_samtoolsdepth_input = sexdeterrmine_bam - .combine(sexdeterrmine_bed) - .multiMap{ - meta, bam, meta2, bed -> - bam = [ meta, bam ] - intervals = [ meta2, bed ] + ch_samtoolsdepth_input = sexdeterrmine_bam + .combine(sexdeterrmine_bed) + .multiMap{ + meta, bam, meta2, bed -> + bam : [ meta, bam ] + intervals : [ meta2, bed ] } - SAMTOOLS_DEPTH(ch_samtoolsdepth_input) - ch_sex_determine_input = SAMTOOLS_DEPTH.out.tsv - ch_versions = ch_versions.mix( SAMTOOLS_DEPTH.out.versions ) + SAMTOOLS_DEPTH(ch_samtoolsdepth_input) + ch_sex_determine_input = SAMTOOLS_DEPTH.out.tsv + ch_versions = ch_versions.mix( SAMTOOLS_DEPTH.out.versions ) - // Run sex determination with samtools depth input - // Does it need a sample list file to run? - SEXDETERRMINE(ch_sex_determine_input) - ch_coverages = SEXDETERRMINE.out.tsv - ch_multiqc_files = ch_multiqc_files.mix( SEXDETERRMINE.out.json ) - ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) - } + // Run sex determination with samtools depth input + // Does it need a sample list file to run? + SEXDETERRMINE(ch_sex_determine_input, sexdeterrmine_list) + ch_coverages = SEXDETERRMINE.out.tsv + ch_multiqc_files = ch_multiqc_files.mix( SEXDETERRMINE.out.json ) + ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) emit: - covergages = ch_coverages // channel: [ val(meta), path("*.tsv") ] + coverages = ch_coverages // channel: [ val(meta), path("*.tsv") ] mqc = ch_multiqc_files // channel: [ val(meta), path("*.json") ] versions = ch_versions // channel: path(versions.yml) } diff --git a/workflows/eager.nf b/workflows/eager.nf index 8024f354b..e91c84d9c 100644 --- a/workflows/eager.nf +++ b/workflows/eager.nf @@ -76,6 +76,7 @@ include { MANIPULATE_DAMAGE } from '../subworkflows/local/manipulate include { METAGENOMICS_COMPLEXITYFILTER } from '../subworkflows/local/metagenomics_complexityfilter' include { ESTIMATE_CONTAMINATION } from '../subworkflows/local/estimate_contamination' include { CALCULATE_DAMAGE } from '../subworkflows/local/calculate_damage' +include { RUN_SEXDETERMINE } from '../subworkflows/local/sex_determination' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -161,6 +162,27 @@ workflow EAGER { // Contamination estimation hapmap_file = file(params.contamination_estimation_angsd_hapmap, checkIfExists:true) + // Sex determine + if ( params.sexdeterrmine_bedfile ) { + ch_sexdeterrmine_bed = Channel.fromPath( params.sexdeterrmine_bedfile, checkIfExists: true ) + .collect() + .map { + file -> + meta = file.simpleName + [meta,file] + } + + } else { + ch_sexdeterrmine_bed = [] + } + + if ( params.sexdeterrmine_samplelist) { + ch_sexdeterrmine_samplelist = Channel.fromPath( params.sexdeterrmine_samplelist, checkIfExists: true ) + + } else { + ch_sexdeterrmine_samplelist = [] + } + // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // @@ -474,6 +496,24 @@ workflow EAGER { } + // + // SUBWORKFLOW: Sex Determine + // + + if ( params.run_sexdeterrmine ) { + + ch_sexdeterrmine_input = ch_dedupped_bams + .map { + meta, bam, bai -> + [ meta, bam ] + } + + RUN_SEXDETERMINE(ch_sexdeterrmine_input, ch_sexdeterrmine_bed, ch_sexdeterrmine_samplelist) + ch_versions = ch_versions.mix( RUN_SEXDETERMINE.out.versions ) + ch_multiqc_files = ch_multiqc_files.mix(RUN_SEXDETERMINE.out.mqc.collect{it[1]}.ifEmpty([])) + + } + // // SUBWORKFLOW: Contamination estimation // From bedc01fc513baadf91b98dd089b94c1a7b98b04c Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 8 Dec 2023 11:32:52 +0100 Subject: [PATCH 04/38] adding module configs --- CITATIONS.md | 3 +++ conf/modules.config | 4 +-- conf/test_humanbam.config | 4 +-- docs/output.md | 16 ++++++++++++ nextflow.config | 1 - nextflow_schema.json | 51 +++++++++++++++++++++++++-------------- 6 files changed, 56 insertions(+), 23 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 63f3adda3..67cde715d 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -99,8 +99,11 @@ > QualiMap Okonechnikov, K., Conesa, A., & García-Alcalde, F. (2016). Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data. Bioinformatics , 32(2), 292–294. Download: http://qualimap.bioinfo.cipf.es/ - [DamageProfiler](https://doi.org/10.1093/bioinformatics/btab190) + > DamageProfiler Neukamm, J., Peltzer, A., & Nieselt, K. (2020). DamageProfiler: Fast damage pattern calculation for ancient DNA. In Bioinformatics (btab190). doi: [10.1093/bioinformatics/btab190](https://doi.org/10.1093/bioinformatics/btab190). Download: https://github.com/Integrative-Transcriptomics/DamageProfiler + - [Sex.DetERRmine.py](https://github.com/TCLamnidis/Sex.DetERRmine.git) Sex.DetERRmine.py Lamnidis, T.C. et al., 2018. Ancient Fennoscandian genomes reveal origin and spread of Siberian ancestry in Europe. Nature communications, 9(1), p.5018. Available at: http://dx.doi.org/10.1038/s41467-018-07483-5. Download: https://github.com/TCLamnidis/Sex.DetERRmine.git + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) diff --git a/conf/modules.config b/conf/modules.config index 19c6b8423..ac3dd7adf 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -850,8 +850,8 @@ process { // SEXDETERRMINE // withName: SAMTOOLS_DEPTH { - tag = { "${meta.reference}|${meta.sample_id}_${meta.library_id}" } - ext.prefix = { "${meta.sample_id}_${meta.library_id}_samtoolsdepth" } + tag = { "${meta1.reference}|${meta1.sample_id}_${meta1.library_id}" } + ext.prefix = { "${meta1.sample_id}_${meta1.library_id}_samtoolsdepth" } publishDir = [ path: { "${params.outdir}/sex_determine/samtools_depth/" }, mode: params.publish_dir_mode, diff --git a/conf/test_humanbam.config b/conf/test_humanbam.config index c6f120efb..35b8025c2 100644 --- a/conf/test_humanbam.config +++ b/conf/test_humanbam.config @@ -33,8 +33,8 @@ params { // TODO Reactivate sexDet and genotyping params when those steps get implemented. // //Sex Determination - //sexdsexdeterrmine_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' - sexdeterrmine_bedfile = '../1240K.pos.list_hs37d5.0based.bed.gz' + sexdeterrmine_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' + //sexdeterrmine_bedfile = '../1240K.pos.list_hs37d5.0based.bed.gz' // // Genotyping // pileupcaller_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' // pileupcaller_snpfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K_covered_in_JK2067_downsampled_s0.1.numeric_chromosomes.snp' diff --git a/docs/output.md b/docs/output.md index d84c9ec02..57a2ff467 100644 --- a/docs/output.md +++ b/docs/output.md @@ -513,3 +513,19 @@ is a tool which calculates a variety of standard 'aDNA' metrics from a BAM file. [ANGSD](http://www.popgen.dk/angsd/index.php/ANGSD) is a software for analyzing next generation sequencing data. Among other functions, ANGSD can estimate contamination for chromosomes for which one copy exists, i.e. X-chromosome for humans with karyotype XY. To do this, we first generate a binary count file for the X-chromosome (`angsd`) and then perform a Fisher's exact test for finding a p-value and jackknife to get an estimate of contamination (`contamination`). Contamination is estimated with Method of Moments (MOM) and Maximum Likelihood (ML) for both Method1 and Method2. Method1 compares the total number of minor and major reads at SNP sites with the number of minor and major reads at adjacent sites, assuming independent errors between reads and sites, while Method2 only samples one read at each site to remove the previous assumption. The results of all methods for each library, as well as respective standard errors are summarised in `nuclear_contamination.txt` and `nuclear_contamination_mqc.json`. + +### Sex Determination + +
+Output files + +- `sex_determination/`: this contains the output for the sex determination run. This is a single `.tsv` file that includes a table with the sample name, the number of autosomal SNPs, number of SNPs on the X/Y chromosome, the number of reads mapping to the autosomes, the number of reads mapping to the X/Y chromosome, the relative coverage on the X/Y chromosomes, and the standard error associated with the relative coverages. These measures are provided for each bam file, one row per file. If the `sexdeterrmine_bedfile` option has not been provided, the error bars cannot be trusted, and runtime will be considerably longer. +-
+ +#### [Sex.DetERRmine](https://nf-co.re/eager/2.5.0/docs/output#sexdeterrmine) + +Sex.DetERRmine calculates the coverage of your mapped reads on the X and Y chromosomes relative to the coverage on the autosomes (X-/Y-rate). This metric can be thought of as the number of copies of chromosomes X and Y that is found within each cell, relative to the autosomal copies. The number of autosomal copies is assumed to be two, meaning that an X-rate of 1.0 means there are two X chromosomes in each cell, while 0.5 means there is a single copy of the X chromosome per cell. Human females have two copies of the X chromosome and no Y chromosome (XX), while human males have one copy of each of the X and Y chromosomes (XY). + +When a bedfile of specific sites is provided, Sex.DetERRmine additionally calculates error bars around each relative coverage estimate. For this estimate to be trustworthy, the sites included in the bedfile should be spaced apart enough that a single sequencing read cannot overlap multiple sites. Hence, when a bedfile has not been provided, this error should be ignored. When a suitable bedfile is provided, each observation of a covered site is independent, and the error around the coverage is equal to the binomial error estimate. This error is then propagated during the calculation of relative coverage for the X and Y chromosomes. + +> Note that in nf-core/eager this will be run on single- and double-stranded variants of the same library separately. This can also help assess for differential contamination between libraries. diff --git a/nextflow.config b/nextflow.config index a848ae244..4bc2f0a68 100644 --- a/nextflow.config +++ b/nextflow.config @@ -200,7 +200,6 @@ params { // Sex determine run_sexdeterrmine = true sexdeterrmine_bedfile = null - sexdeterrmine_samplelist = null } diff --git a/nextflow_schema.json b/nextflow_schema.json index 904c7ef61..bd51e929b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -214,14 +214,12 @@ "type": "boolean", "description": "Display help text.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "version": { "type": "boolean", "description": "Display version and exit.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "publish_dir_mode": { @@ -245,7 +243,6 @@ "type": "boolean", "description": "Send plain-text email instead of HTML.", "fa_icon": "fas fa-remove-format", - "default": false, "hidden": true }, "max_multiqc_email_size": { @@ -260,7 +257,6 @@ "type": "boolean", "description": "Do not use coloured log outputs.", "fa_icon": "fas fa-palette", - "default": false, "hidden": true }, "hook_url": { @@ -299,7 +295,6 @@ "type": "boolean", "fa_icon": "far fa-eye-slash", "description": "Show all params when using `--help`", - "default": false, "hidden": true, "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." }, @@ -307,7 +302,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters fails when an unrecognised parameter is found.", - "default": false, "hidden": true, "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." }, @@ -315,7 +309,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters in lenient more.", - "default": false, "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." } @@ -960,8 +953,7 @@ "fa_icon": "fab fa-creative-commons-sampling-plus" }, "skip_qualimap": { - "type": "boolean", - "default": "false" + "type": "boolean" }, "snpcapture_bed": { "type": "string", @@ -1107,6 +1099,29 @@ "fa_icon": "fas fa-map" } } + }, + "human_sex_determination": { + "title": "Human Sex Determination", + "type": "object", + "description": "Options for the calculation of biological sex of human individuals.", + "default": "", + "properties": { + "run_sexdeterrmine": { + "type": "boolean", + "default": true, + "fa_icon": "fas fa-transgender-alt", + "description": "Turn on sex determination for human reference genomes. This will run on single- and double-stranded variants of a library separately.", + "help_text": "Specify to run the optional process of sex determination." + }, + "sexdeterrmine_bedfile": { + "type": "string", + "fa_icon": "fas fa-bed", + "description": "Specify path to SNP panel in bed format for error bar calculation. Optional (see documentation).", + "help_text": "Specify an optional bedfile of the list of SNPs to be used for X-/Y-rate calculation. Running without this parameter will considerably increase runtime, and render the resulting error bars untrustworthy. Theoretically, any set of SNPs that are distant enough that two SNPs are unlikely to be covered by the same read can be used here. The programme was coded with the 1240K panel in mind. The path must be in quotes." + } + }, + "fa_icon": "fas fa-transgender-alt", + "help_text": "" } }, "allOf": [ @@ -1131,9 +1146,6 @@ { "$ref": "#/definitions/mapping" }, - { - "$ref": "#/definitions/adna_damage_analysis" - }, { "$ref": "#/definitions/bam_filtering" }, @@ -1144,28 +1156,31 @@ "$ref": "#/definitions/deduplication" }, { - "$ref": "#/definitions/mitochondrial_to_nuclear_ratio" + "$ref": "#/definitions/damage_manipulation" }, { - "$ref": "#/definitions/mapping_statistics" + "$ref": "#/definitions/genotyping" }, { - "$ref": "#/definitions/damage_manipulation" + "$ref": "#/definitions/mitochondrial_to_nuclear_ratio" }, { - "$ref": "#/definitions/genotyping" + "$ref": "#/definitions/mapping_statistics" }, { "$ref": "#/definitions/adna_damage_analysis" }, { - "$ref": "#/definitions/contamination_estimation" + "$ref": "#/definitions/feature_annotation_statistics" }, { "$ref": "#/definitions/host_removal" }, { - "$ref": "#/definitions/feature_annotation_statistics" + "$ref": "#/definitions/contamination_estimation" + }, + { + "$ref": "#/definitions/human_sex_determination" } ] } From 4b1272784d7512b489e13bb245bd8be9848ca6a6 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 8 Dec 2023 11:38:57 +0100 Subject: [PATCH 05/38] linting --- .github/CONTRIBUTING.md | 1 + assets/nf-core-eager_logo_light.png | Bin 68825 -> 10462 bytes lib/NfcoreTemplate.groovy | 2 +- 3 files changed, 2 insertions(+), 1 deletion(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 7f42e77a6..4edb21605 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -116,3 +116,4 @@ To get started: Devcontainer specs: - [DevContainer config](.devcontainer/devcontainer.json) +- [Dockerfile](.devcontainer/Dockerfile) diff --git a/assets/nf-core-eager_logo_light.png b/assets/nf-core-eager_logo_light.png index f7c3062e407b6a1e9c85890bb0b94759531a8cb8..271bbedd3e47b699635961c9e52cec2add1f0f3b 100644 GIT binary patch literal 10462 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params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] def sf = new File("$projectDir/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) From 7073bfe1e7e283c9d40b9cff29edb283c7ae7385 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 8 Dec 2023 11:48:34 +0100 Subject: [PATCH 06/38] Adding sex determine subworfflow --- .github/CONTRIBUTING.md | 5 +- .github/workflows/linting.yml | 2 +- CODE_OF_CONDUCT.md | 133 +++++++++++++++++++++------- assets/nf-core-eager_logo_light.png | Bin 10462 -> 68825 bytes lib/NfcoreTemplate.groovy | 18 +++- 5 files changed, 123 insertions(+), 35 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 4edb21605..fa933e118 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,7 +9,9 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). +:::info +If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). +::: ## Contribution workflow @@ -116,4 +118,3 @@ To get started: Devcontainer specs: - [DevContainer config](.devcontainer/devcontainer.json) -- [Dockerfile](.devcontainer/Dockerfile) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 888cb4bc3..b8bdd2143 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -78,7 +78,7 @@ jobs: - uses: actions/setup-python@v4 with: - python-version: "3.8" + python-version: "3.11" architecture: "x64" - name: Install dependencies diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index f4fd052f1..c089ec78c 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,18 +1,20 @@ -# Code of Conduct at nf-core (v1.0) +# Code of Conduct at nf-core (v1.4) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age +- Ability - Body size +- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Physical and neurological ability +- Neurodiversity - Race or ethnicity - Religion - Sexual identity and orientation @@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. ## Our Responsibilities -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. -## When are where does this Code of Conduct apply? +## When and where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Accept feedback, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) - Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for Reporting CoC violations +## Procedures for reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. -All reports will be handled with utmost discretion and confidentially. +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. ## Attribution and Acknowledgements @@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially. ## Changelog -### v1.0 - March 12th, 2021 +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/assets/nf-core-eager_logo_light.png b/assets/nf-core-eager_logo_light.png index 271bbedd3e47b699635961c9e52cec2add1f0f3b..f7c3062e407b6a1e9c85890bb0b94759531a8cb8 100644 GIT binary patch literal 68825 zcmeEt`9IX_`~RRQS?ZKSWhn*~p=96c5GGq9OZHNfecuPCQz(&w!1jG0qM))u18{N;szxKLnntC7*Z0~7*=;B1!jv^4p5Gb_^hQ29NgTYTSd@O|5 zS3HI44fR<@BwC_WweNAg^K`t?ay|Ua^`zuS;o*5X;p5j0nLR_3TdTw-*C$<<{Vk$; z9`%au>-b1%=CCl=x~!Jp!Br{RFpzjKp!3X+Tb;*QRKss@Kb){h^c+@seV?p-3zMBT zv9)Zlu({<`v3Pc z_~QTk@G~L)&kz6ShyTBGp!b^mFYH1%8g&}PE+NMRdy{Rgwkaa9QvrRQY2HJz)6`6H 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zs_=N=&nlb#B|qSaD$?e0mX7awIjPS>HogmED;4`j*Uvj8AKwyWkg(kG)Q^h$J0us@ z|4gpp(M*arM}Z(p{}J_&I9=1zG${S`kjkW(8Cd4MkpOVcV;T{m1#*azV#_!&Wp+7_ zX2BOK955N=qYp+2Gv;8?O5x||3qB-BJ<|nG5k;=w2?N;&I5B|y*6r;Ea)bkX>ZsH7 z0k960FI4!C$si07D(Qc9shLJ4bUEQ{!>srnY?-SPa5D=(5>IV1$lOfRn<8iuVAX-jxEcKp985?2{iPm@e#~wWDq0lMqCAzTgoR`0mRbK0O}3z zr)sALQ>w6^tB5TsGsTQ)FN!8{{7Af?GI845P2zHhP3O=?>1Xf;_ z6zc~}c-;JQ38X`+#v%I&qa0*`_aG`7GX?iHk%Rwuz3(zV*1j^-AQv|Kppr;2}00T`(YDKXJNJ!fTf=eqd$}=$R6!gi86=wnx0!A7o+E2 z`nD3A{Cs$$)-3{6$}-OJY!VsemcJPM#&Jdb?8!+69R2lLwaDr zub!r=$Qq!9jc(fLMOeCqDsqv`fOX9H5PQnQuce}t=O6(_W}jt-qIAu+0ou=M06yA* z&4Q)Fw5DYNFjIS5OOEw)nc)qUuzeXn(gLW%bWCd=`<3+OCXOGCaM4`D+S&l4bA_26# sslC)l#$OTjKUt0be_3GZ#yxh_ckAcF Date: Fri, 8 Dec 2023 11:55:02 +0100 Subject: [PATCH 07/38] rerunning prettier after merge --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index f1bd49463..a4839ca36 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1196,7 +1196,7 @@ }, { "$ref": "#/definitions/human_sex_determination" - }, + }, { "$ref": "#/definitions/contamination_estimation" } From 24d0a889f78e7643a1cd345ef33ed0cc1f17f18b Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 8 Dec 2023 11:58:41 +0100 Subject: [PATCH 08/38] fixing linting --- modules.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules.json b/modules.json index e5318cb55..353b1afe5 100644 --- a/modules.json +++ b/modules.json @@ -219,7 +219,7 @@ "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] - } + }, "sexdeterrmine": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", From 1c2cb3751c1eb87489f396e65d75f27249e28134 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 26 Jan 2024 10:05:00 +0100 Subject: [PATCH 09/38] Apply suggestions from code review Co-authored-by: Thiseas C. Lamnidis --- CITATIONS.md | 4 +++- conf/modules.config | 4 +--- conf/test_humanbam.config | 1 - docs/output.md | 6 +++--- nextflow.config | 2 +- nextflow_schema.json | 2 +- subworkflows/local/sex_determination.nf | 3 +-- workflows/eager.nf | 2 +- 8 files changed, 11 insertions(+), 13 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 6e02cb451..2353dbbad 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -106,7 +106,9 @@ > DamageProfiler Neukamm, J., Peltzer, A., & Nieselt, K. (2020). DamageProfiler: Fast damage pattern calculation for ancient DNA. In Bioinformatics (btab190). doi: [10.1093/bioinformatics/btab190](https://doi.org/10.1093/bioinformatics/btab190). Download: https://github.com/Integrative-Transcriptomics/DamageProfiler - - [Sex.DetERRmine.py](https://github.com/TCLamnidis/Sex.DetERRmine.git) Sex.DetERRmine.py Lamnidis, T.C. et al., 2018. Ancient Fennoscandian genomes reveal origin and spread of Siberian ancestry in Europe. Nature communications, 9(1), p.5018. Available at: http://dx.doi.org/10.1038/s41467-018-07483-5. Download: https://github.com/TCLamnidis/Sex.DetERRmine.git + - [Sex.DetERRmine.py](http://dx.doi.org/10.1038/s41467-018-07483-5) + + > Sex.DetERRmine.py Lamnidis, T.C. et al., 2018. Ancient Fennoscandian genomes reveal origin and spread of Siberian ancestry in Europe. Nature communications, 9(1), p.5018. Available at: http://dx.doi.org/10.1038/s41467-018-07483-5. Download: https://github.com/TCLamnidis/Sex.DetERRmine ## Software packaging/containerisation tools diff --git a/conf/modules.config b/conf/modules.config index 8d7219ea0..26dd69e4e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -891,9 +891,7 @@ process { tag = { "${meta1.reference}|${meta1.sample_id}_${meta1.library_id}" } ext.prefix = { "${meta1.sample_id}_${meta1.library_id}_samtoolsdepth" } publishDir = [ - path: { "${params.outdir}/sex_determine/samtools_depth/" }, - mode: params.publish_dir_mode, - enabled: true + enabled: false ] } diff --git a/conf/test_humanbam.config b/conf/test_humanbam.config index ff8bd23e9..b21d58206 100644 --- a/conf/test_humanbam.config +++ b/conf/test_humanbam.config @@ -37,7 +37,6 @@ params { // TODO Reactivate sexDet and genotyping params when those steps get implemented. // //Sex Determination sexdeterrmine_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' - //sexdeterrmine_bedfile = '../1240K.pos.list_hs37d5.0based.bed.gz' // // Genotyping // pileupcaller_bedfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz' // pileupcaller_snpfile = 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K_covered_in_JK2067_downsampled_s0.1.numeric_chromosomes.snp' diff --git a/docs/output.md b/docs/output.md index 049915253..e7cc93f01 100644 --- a/docs/output.md +++ b/docs/output.md @@ -526,13 +526,13 @@ is a tool which calculates a variety of standard 'aDNA' metrics from a BAM file.

Output files -- `sex_determination/`: this contains the output for the sex determination run. This is a single `.tsv` file that includes a table with the sample name, the number of autosomal SNPs, number of SNPs on the X/Y chromosome, the number of reads mapping to the autosomes, the number of reads mapping to the X/Y chromosome, the relative coverage on the X/Y chromosomes, and the standard error associated with the relative coverages. These measures are provided for each bam file, one row per file. If the `sexdeterrmine_bedfile` option has not been provided, the error bars cannot be trusted, and runtime will be considerably longer. +- `sex_determination/`: this contains the output for the sex determination run. This is a single `.tsv` file that includes a table with the sample name, the number of autosomal SNPs, number of SNPs on the X/Y chromosome, the number of reads mapping to the autosomes, the number of reads mapping to the X/Y chromosome, the relative coverage on the X/Y chromosomes, and the standard error associated with the relative coverages. These measures are provided for each bam file, one row per file. If the `sexdeterrmine_bedfile` option has not been provided, the error bars cannot be trusted! -
-#### [Sex.DetERRmine](https://nf-co.re/eager/2.5.0/docs/output#sexdeterrmine) +#### Sex.DetERRmine Sex.DetERRmine calculates the coverage of your mapped reads on the X and Y chromosomes relative to the coverage on the autosomes (X-/Y-rate). This metric can be thought of as the number of copies of chromosomes X and Y that is found within each cell, relative to the autosomal copies. The number of autosomal copies is assumed to be two, meaning that an X-rate of 1.0 means there are two X chromosomes in each cell, while 0.5 means there is a single copy of the X chromosome per cell. Human females have two copies of the X chromosome and no Y chromosome (XX), while human males have one copy of each of the X and Y chromosomes (XY). -When a bedfile of specific sites is provided, Sex.DetERRmine additionally calculates error bars around each relative coverage estimate. For this estimate to be trustworthy, the sites included in the bedfile should be spaced apart enough that a single sequencing read cannot overlap multiple sites. Hence, when a bedfile has not been provided, this error should be ignored. When a suitable bedfile is provided, each observation of a covered site is independent, and the error around the coverage is equal to the binomial error estimate. This error is then propagated during the calculation of relative coverage for the X and Y chromosomes. +When a bedfile of specific sites is provided, Sex.DetERRmine runs much faster and additionally calculates error bars around each relative coverage estimate. For this estimate to be trustworthy, the sites included in the bedfile should be spaced apart enough that a single sequencing read cannot overlap multiple sites. Hence, when a bedfile has not been provided, this error should be ignored. When a suitable bedfile is provided, each observation of a covered site is independent, and the error around the coverage is equal to the binomial error estimate. This error is then propagated during the calculation of relative coverage for the X and Y chromosomes. > Note that in nf-core/eager this will be run on single- and double-stranded variants of the same library separately. This can also help assess for differential contamination between libraries. diff --git a/nextflow.config b/nextflow.config index 9245451fa..e063a02db 100644 --- a/nextflow.config +++ b/nextflow.config @@ -201,7 +201,7 @@ params { // Genotyping genotyping_source = 'raw' - // Sex determine + // Sex determination run_sexdeterrmine = true sexdeterrmine_bedfile = null diff --git a/nextflow_schema.json b/nextflow_schema.json index a4839ca36..a8b9d6b87 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1132,7 +1132,7 @@ "type": "string", "fa_icon": "fas fa-bed", "description": "Specify path to SNP panel in bed format for error bar calculation. Optional (see documentation).", - "help_text": "Specify an optional bedfile of the list of SNPs to be used for X-/Y-rate calculation. Running without this parameter will considerably increase runtime, and render the resulting error bars untrustworthy. Theoretically, any set of SNPs that are distant enough that two SNPs are unlikely to be covered by the same read can be used here. The programme was coded with the 1240K panel in mind. The path must be in quotes." + "help_text": "Specify an optional bedfile of the list of SNPs to be used for X-/Y-rate calculation. Running without this parameter will considerably increase runtime, and render the resulting error bars untrustworthy. Theoretically, any set of SNPs that are distant enough that two SNPs are unlikely to be covered by the same read can be used here. The programme was coded with the 1240K panel in mind." } }, "fa_icon": "fas fa-transgender-alt", diff --git a/subworkflows/local/sex_determination.nf b/subworkflows/local/sex_determination.nf index 7aaf6ba63..620bc38fc 100644 --- a/subworkflows/local/sex_determination.nf +++ b/subworkflows/local/sex_determination.nf @@ -11,7 +11,6 @@ workflow RUN_SEXDETERMINE { // samtoolsdepth_input // channel: [ val(meta1), [ bam ], [ meta2 ] [ intervals_bed ] ] sexdeterrmine_bam // channel: [ val(meta1), [ bam ] ] sexdeterrmine_bed // channel: [ [ meta2 ] [ intervals_bed ] ] - sexdeterrmine_list // channel: [ val(meta), [ sample_list_file ] ] main: ch_versions = Channel.empty() @@ -41,7 +40,7 @@ workflow RUN_SEXDETERMINE { ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) emit: - coverages = ch_coverages // channel: [ val(meta), path("*.tsv") ] + coverages = ch_coverages // channel: [ val(meta), path("*.tsv") ] mqc = ch_multiqc_files // channel: [ val(meta), path("*.json") ] versions = ch_versions // channel: path(versions.yml) } diff --git a/workflows/eager.nf b/workflows/eager.nf index b86de905a..7e09d1bf8 100644 --- a/workflows/eager.nf +++ b/workflows/eager.nf @@ -547,7 +547,7 @@ workflow EAGER { RUN_SEXDETERMINE(ch_sexdeterrmine_input, ch_sexdeterrmine_bed, ch_sexdeterrmine_samplelist) ch_versions = ch_versions.mix( RUN_SEXDETERMINE.out.versions ) - ch_multiqc_files = ch_multiqc_files.mix(RUN_SEXDETERMINE.out.mqc.collect{it[1]}.ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix( RUN_SEXDETERMINE.out.mqc.collect{it[1]}.ifEmpty([]) ) } From b805c83573ba3ddc79cc48e73289ea3fe28a9bb9 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 26 Jan 2024 10:47:29 +0100 Subject: [PATCH 10/38] adding parameters for samtools depth --- conf/modules.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/modules.config b/conf/modules.config index 26dd69e4e..652963036 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -890,6 +890,7 @@ process { withName: SAMTOOLS_DEPTH { tag = { "${meta1.reference}|${meta1.sample_id}_${meta1.library_id}" } ext.prefix = { "${meta1.sample_id}_${meta1.library_id}_samtoolsdepth" } + ext.args = '-aa -q30 -Q30' publishDir = [ enabled: false ] From c9d4788e753db123c7953a4b7aa4e4c7c6f01dd6 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 26 Jan 2024 11:15:24 +0100 Subject: [PATCH 11/38] Header option --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 652963036..13f50f898 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -890,7 +890,7 @@ process { withName: SAMTOOLS_DEPTH { tag = { "${meta1.reference}|${meta1.sample_id}_${meta1.library_id}" } ext.prefix = { "${meta1.sample_id}_${meta1.library_id}_samtoolsdepth" } - ext.args = '-aa -q30 -Q30' + ext.args = '-aa -q30 -Q30 -H' publishDir = [ enabled: false ] From 4d1f7652507aaa62b966a45158928ad5141fb822 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 26 Jan 2024 11:15:44 +0100 Subject: [PATCH 12/38] importing properly samtools module --- conf/modules.config | 5 +++-- subworkflows/local/sex_determination.nf | 10 +++++----- 2 files changed, 8 insertions(+), 7 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 13f50f898..e1651f0fd 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -887,12 +887,12 @@ process { // // SEXDETERRMINE // - withName: SAMTOOLS_DEPTH { + withName: SAMTOOLS_DEPTH_SEXDETERMINE { tag = { "${meta1.reference}|${meta1.sample_id}_${meta1.library_id}" } ext.prefix = { "${meta1.sample_id}_${meta1.library_id}_samtoolsdepth" } ext.args = '-aa -q30 -Q30 -H' publishDir = [ - enabled: false + enabled: false ] } @@ -902,6 +902,7 @@ process { publishDir = [ path: { "${params.outdir}/sex_determine/sexdeterrmine/" }, mode: params.publish_dir_mode, + pattern: '*{_sexdetermine}*', enabled: true ] } diff --git a/subworkflows/local/sex_determination.nf b/subworkflows/local/sex_determination.nf index 620bc38fc..b20c9cdd7 100644 --- a/subworkflows/local/sex_determination.nf +++ b/subworkflows/local/sex_determination.nf @@ -2,8 +2,8 @@ // Run sex determine // -include { SAMTOOLS_DEPTH } from '../../modules/nf-core/samtools/depth/main' -include { SEXDETERRMINE } from '../../modules/nf-core/sexdeterrmine/main' +include { SAMTOOLS_DEPTH as SAMTOOLS_DEPTH_SEXDETERMINE } from '../../modules/nf-core/samtools/depth/main' +include { SEXDETERRMINE } from '../../modules/nf-core/sexdeterrmine/main' workflow RUN_SEXDETERMINE { @@ -28,9 +28,9 @@ workflow RUN_SEXDETERMINE { intervals : [ meta2, bed ] } - SAMTOOLS_DEPTH(ch_samtoolsdepth_input) - ch_sex_determine_input = SAMTOOLS_DEPTH.out.tsv - ch_versions = ch_versions.mix( SAMTOOLS_DEPTH.out.versions ) + SAMTOOLS_DEPTH_SEXDETERMINE(ch_samtoolsdepth_input) + ch_sex_determine_input = SAMTOOLS_DEPTH_SEXDETERMINE.out.tsv + ch_versions = ch_versions.mix( SAMTOOLS_DEPTH_SEXDETERMINE.out.versions ) // Run sex determination with samtools depth input // Does it need a sample list file to run? From b36bc45877181079644572a8509465ec47cf57d7 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 26 Jan 2024 12:03:04 +0100 Subject: [PATCH 13/38] trying to add bed from reference file --- ...ermination.nf => run_sex_determination.nf} | 30 ++++++++++---- workflows/eager.nf | 41 +++++-------------- 2 files changed, 32 insertions(+), 39 deletions(-) rename subworkflows/local/{sex_determination.nf => run_sex_determination.nf} (59%) diff --git a/subworkflows/local/sex_determination.nf b/subworkflows/local/run_sex_determination.nf similarity index 59% rename from subworkflows/local/sex_determination.nf rename to subworkflows/local/run_sex_determination.nf index b20c9cdd7..e522bf9db 100644 --- a/subworkflows/local/sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -20,21 +20,33 @@ workflow RUN_SEXDETERMINE { // Generate sex_determine input // Break here the input not in the workflow - ch_samtoolsdepth_input = sexdeterrmine_bam - .combine(sexdeterrmine_bed) - .multiMap{ - meta, bam, meta2, bed -> - bam : [ meta, bam ] - intervals : [ meta2, bed ] - } + ch_bed = sexdeterrmine_bed + .map { + // Prepend a new meta that contains the meta.id value as the new_meta.reference attribute + WorkflowEager.addNewMetaFromAttributes( it, "id" , "reference" , false ) + } + + ch_input_sexdetermine = sexdeterrmine_bam + .map { + // Prepend a new meta that contains the meta.reference value as the new_meta.reference attribute + WorkflowEager.addNewMetaFromAttributes( it, "reference" , "reference" , false ) + } + .combine( ch_bed, by: 0 ) // [ [combine_meta], [meta], bam, bai, [ref_meta], bed ] + + ch_samtoolsdepth_input = ch_input_sexdetermine + .multiMap { + ignore_me, meta, bam, bai, ref_meta, bed -> + bam: [ meta, bam ] + bed: [ ref_meta, bed ] + } SAMTOOLS_DEPTH_SEXDETERMINE(ch_samtoolsdepth_input) ch_sex_determine_input = SAMTOOLS_DEPTH_SEXDETERMINE.out.tsv ch_versions = ch_versions.mix( SAMTOOLS_DEPTH_SEXDETERMINE.out.versions ) // Run sex determination with samtools depth input - // Does it need a sample list file to run? - SEXDETERRMINE(ch_sex_determine_input, sexdeterrmine_list) + // Does it need a sample list file to run? yep + SEXDETERRMINE(ch_sex_determine_input) ch_coverages = SEXDETERRMINE.out.tsv ch_multiqc_files = ch_multiqc_files.mix( SEXDETERRMINE.out.json ) ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) diff --git a/workflows/eager.nf b/workflows/eager.nf index 7e09d1bf8..5aa4f89dd 100644 --- a/workflows/eager.nf +++ b/workflows/eager.nf @@ -71,7 +71,7 @@ include { MANIPULATE_DAMAGE } from '../subworkflows/local/manipulate include { METAGENOMICS_COMPLEXITYFILTER } from '../subworkflows/local/metagenomics_complexityfilter' include { ESTIMATE_CONTAMINATION } from '../subworkflows/local/estimate_contamination' include { CALCULATE_DAMAGE } from '../subworkflows/local/calculate_damage' -include { RUN_SEXDETERMINE } from '../subworkflows/local/sex_determination' +include { RUN_SEXDETERMINE } from '../subworkflows/local/run_sex_determination' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -134,25 +134,12 @@ workflow EAGER { } - // Sex determine + // SUBWORKFLOW: Run Sex determination if ( params.sexdeterrmine_bedfile ) { - ch_sexdeterrmine_bed = Channel.fromPath( params.sexdeterrmine_bedfile, checkIfExists: true ) - .collect() - .map { - file -> - meta = file.simpleName - [meta,file] - } - - } else { - ch_sexdeterrmine_bed = [] - } - - if ( params.sexdeterrmine_samplelist) { - ch_sexdeterrmine_samplelist = Channel.fromPath( params.sexdeterrmine_samplelist, checkIfExists: true ) - - } else { - ch_sexdeterrmine_samplelist = [] + ch_sexdeterrmine_bed = REFERENCE_INDEXING.out.sexdeterrmine_bed + .map{ + WorkflowEager.addNewMetaFromAttributes( it, "id" , "reference" , false ) + } } // @@ -534,21 +521,15 @@ workflow EAGER { } // - // SUBWORKFLOW: Sex Determine + // SUBWORKFLOW: Run Sex Determination // if ( params.run_sexdeterrmine ) { + ch_sexdeterrmine_input = ch_dedupped_bams - ch_sexdeterrmine_input = ch_dedupped_bams - .map { - meta, bam, bai -> - [ meta, bam ] - } - - RUN_SEXDETERMINE(ch_sexdeterrmine_input, ch_sexdeterrmine_bed, ch_sexdeterrmine_samplelist) - ch_versions = ch_versions.mix( RUN_SEXDETERMINE.out.versions ) - ch_multiqc_files = ch_multiqc_files.mix( RUN_SEXDETERMINE.out.mqc.collect{it[1]}.ifEmpty([]) ) - + RUN_SEXDETERMINE(ch_sexdeterrmine_input, REFERENCE_INDEXING.out.sexdeterrmine_bed ) + ch_versions = ch_versions.mix( ESTIMATE_CONTAMINATION.out.versions ) + ch_multiqc_files = ch_multiqc_files.mix( ESTIMATE_CONTAMINATION.out.mqc.collect{it[1]}.ifEmpty([]) ) } // From 839b81c6a11f107eec702f04241f76ee90ce0fa3 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 16 Feb 2024 11:48:25 +0100 Subject: [PATCH 14/38] adding bed to reference index --- subworkflows/local/reference_indexing_single.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/reference_indexing_single.nf b/subworkflows/local/reference_indexing_single.nf index 161d8e91b..c4658a7d4 100644 --- a/subworkflows/local/reference_indexing_single.nf +++ b/subworkflows/local/reference_indexing_single.nf @@ -86,7 +86,7 @@ workflow REFERENCE_INDEXING_SINGLE { def capture_bed = params.snpcapture_bed != null ? file(params.snpcapture_bed, checkIfExists: true ) : "" def pileupcaller_bed = "" def pileupcaller_snp = "" - def sexdet_bed = "" + def sexdet_bed = params.sexdeterrmine_bedfile != null ? file(params.sexdeterrmine_bedfile, checkIfExists: true ) : "" def bedtools_feature = params.mapstats_bedtools_featurefile != null ? file(params.mapstats_bedtools_featurefile, checkIfExists: true ) : "" [ meta, fasta, fai, dict, mapper_index, params.fasta_circular_target, params.mitochondrion_header, contamination_estimation_angsd_hapmap, pmd_masked_fasta, pmd_bed_for_masking, capture_bed, pileupcaller_bed, pileupcaller_snp, sexdet_bed, bedtools_feature ] } From 3dda4e17a0bc618a592585f06f04a9beebbf0d49 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 16 Feb 2024 11:55:31 +0100 Subject: [PATCH 15/38] breaking inputs inside the module with the reference outputs --- CITATIONS.md | 4 +- subworkflows/local/run_sex_determination.nf | 59 +++++++++++---------- workflows/eager.nf | 14 ++--- 3 files changed, 35 insertions(+), 42 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 2353dbbad..058e6e1c7 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -106,8 +106,8 @@ > DamageProfiler Neukamm, J., Peltzer, A., & Nieselt, K. (2020). DamageProfiler: Fast damage pattern calculation for ancient DNA. In Bioinformatics (btab190). doi: [10.1093/bioinformatics/btab190](https://doi.org/10.1093/bioinformatics/btab190). Download: https://github.com/Integrative-Transcriptomics/DamageProfiler - - [Sex.DetERRmine.py](http://dx.doi.org/10.1038/s41467-018-07483-5) - + - [Sex.DetERRmine.py](http://dx.doi.org/10.1038/s41467-018-07483-5) + > Sex.DetERRmine.py Lamnidis, T.C. et al., 2018. Ancient Fennoscandian genomes reveal origin and spread of Siberian ancestry in Europe. Nature communications, 9(1), p.5018. Available at: http://dx.doi.org/10.1038/s41467-018-07483-5. Download: https://github.com/TCLamnidis/Sex.DetERRmine ## Software packaging/containerisation tools diff --git a/subworkflows/local/run_sex_determination.nf b/subworkflows/local/run_sex_determination.nf index e522bf9db..372a2dd03 100644 --- a/subworkflows/local/run_sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -16,40 +16,41 @@ workflow RUN_SEXDETERMINE { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() + if ( params.run_sexdeterrmine ) { + // Generate sex_determine input + // Break here the input not in the workflow + + ch_bed = sexdeterrmine_bed + .map { + // Prepend a new meta that contains the meta.id value as the new_meta.reference attribute + WorkflowEager.addNewMetaFromAttributes( it, "id" , "reference" , false ) + } - // Generate sex_determine input - // Break here the input not in the workflow + ch_input_sexdetermine = sexdeterrmine_bam + .map { + // Prepend a new meta that contains the meta.reference value as the new_meta.reference attribute + WorkflowEager.addNewMetaFromAttributes( it, "reference" , "reference" , false ) + } + .combine( ch_bed, by: 0 ) // [ [combine_meta], [meta], bam, bai, [ref_meta], bed ] - ch_bed = sexdeterrmine_bed - .map { - // Prepend a new meta that contains the meta.id value as the new_meta.reference attribute - WorkflowEager.addNewMetaFromAttributes( it, "id" , "reference" , false ) - } + ch_samtoolsdepth_input = ch_input_sexdetermine + .multiMap { + ignore_me, meta, bam, bai, ref_meta, bed -> + bam: [ meta, bam ] + bed: [ ref_meta, bed ] + } - ch_input_sexdetermine = sexdeterrmine_bam - .map { - // Prepend a new meta that contains the meta.reference value as the new_meta.reference attribute - WorkflowEager.addNewMetaFromAttributes( it, "reference" , "reference" , false ) - } - .combine( ch_bed, by: 0 ) // [ [combine_meta], [meta], bam, bai, [ref_meta], bed ] + SAMTOOLS_DEPTH_SEXDETERMINE(ch_samtoolsdepth_input.bam, ch_samtoolsdepth_input.bed) + ch_sex_determine_input = SAMTOOLS_DEPTH_SEXDETERMINE.out.tsv + ch_versions = ch_versions.mix( SAMTOOLS_DEPTH_SEXDETERMINE.out.versions ) - ch_samtoolsdepth_input = ch_input_sexdetermine - .multiMap { - ignore_me, meta, bam, bai, ref_meta, bed -> - bam: [ meta, bam ] - bed: [ ref_meta, bed ] - } - - SAMTOOLS_DEPTH_SEXDETERMINE(ch_samtoolsdepth_input) - ch_sex_determine_input = SAMTOOLS_DEPTH_SEXDETERMINE.out.tsv - ch_versions = ch_versions.mix( SAMTOOLS_DEPTH_SEXDETERMINE.out.versions ) + // Run sex determination with samtools depth input + SEXDETERRMINE(ch_sex_determine_input, []) + ch_coverages = SEXDETERRMINE.out.tsv + ch_multiqc_files = ch_multiqc_files.mix( SEXDETERRMINE.out.json ) + ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) - // Run sex determination with samtools depth input - // Does it need a sample list file to run? yep - SEXDETERRMINE(ch_sex_determine_input) - ch_coverages = SEXDETERRMINE.out.tsv - ch_multiqc_files = ch_multiqc_files.mix( SEXDETERRMINE.out.json ) - ch_versions = ch_versions.mix( SEXDETERRMINE.out.versions ) + } emit: coverages = ch_coverages // channel: [ val(meta), path("*.tsv") ] diff --git a/workflows/eager.nf b/workflows/eager.nf index 5aa4f89dd..e37547663 100644 --- a/workflows/eager.nf +++ b/workflows/eager.nf @@ -134,14 +134,6 @@ workflow EAGER { } - // SUBWORKFLOW: Run Sex determination - if ( params.sexdeterrmine_bedfile ) { - ch_sexdeterrmine_bed = REFERENCE_INDEXING.out.sexdeterrmine_bed - .map{ - WorkflowEager.addNewMetaFromAttributes( it, "id" , "reference" , false ) - } - } - // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // @@ -527,9 +519,9 @@ workflow EAGER { if ( params.run_sexdeterrmine ) { ch_sexdeterrmine_input = ch_dedupped_bams - RUN_SEXDETERMINE(ch_sexdeterrmine_input, REFERENCE_INDEXING.out.sexdeterrmine_bed ) - ch_versions = ch_versions.mix( ESTIMATE_CONTAMINATION.out.versions ) - ch_multiqc_files = ch_multiqc_files.mix( ESTIMATE_CONTAMINATION.out.mqc.collect{it[1]}.ifEmpty([]) ) + RUN_SEXDETERMINE(ch_sexdeterrmine_input, REFERENCE_INDEXING.out.sexdeterrmine_bed.dump() ) + ch_versions = ch_versions.mix( RUN_SEXDETERMINE.out.versions ) + ch_multiqc_files = ch_multiqc_files.mix( RUN_SEXDETERMINE.out.mqc.collect{it[1]}.ifEmpty([]) ) } // From 09e800fe48e48d0c4a80e22dd88b974403cab90d Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 16 Feb 2024 12:01:29 +0100 Subject: [PATCH 16/38] linting changes --- .github/CONTRIBUTING.md | 3 ++ .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/branch.yml | 2 +- .github/workflows/linting.yml | 65 +++++------------------ .github/workflows/linting_comment.yml | 2 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-eager_logo_light.png | Bin 68825 -> 71836 bytes docs/images/nf-core-eager_logo_dark.png | Bin 68937 -> 26486 bytes docs/images/nf-core-eager_logo_light.png | Bin 68825 -> 22876 bytes lib/NfcoreTemplate.groovy | 32 ++++++----- pyproject.toml | 17 +++--- 12 files changed, 49 insertions(+), 77 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index fa933e118..82ae5a145 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 55768a0c1..2a999dcee 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/eage - [ ] If necessary, also make a PR on the nf-core/eager _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 00f205457..236bc6f6f 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd2143..81cd098e9 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,72 +11,33 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - uses: actions/setup-node@v3 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f2..147bcd10c 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/assets/email_template.html b/assets/email_template.html index 36bfc9c8d..2b66b754d 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/eager v${version}

+

nf-core/eager ${version}

Run Name: $runName

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throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -155,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -227,15 +232,14 @@ class NfcoreTemplate { // Dump pipeline parameters in a json file // public static void dump_parameters(workflow, params) { - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def output_pf = new File(output_d, "params_${timestamp}.json") + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") def jsonStr = JsonOutput.toJson(params) - output_pf.text = JsonOutput.prettyPrint(jsonStr) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() } // diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6f..7d08e1c8e 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] From 79f097e3cb453709c28dfea8f9fd1ceb2599e846 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 23 Feb 2024 10:36:11 +0100 Subject: [PATCH 17/38] prettier --- nextflow_schema.json | 1 - 1 file changed, 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 4c78c3990..b009044a5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1208,7 +1208,6 @@ }, { "$ref": "#/definitions/contamination_estimation" - } ] } From b35a4874585c112f7fd6e41e66adcd1709ea72f2 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 23 Feb 2024 10:39:31 +0100 Subject: [PATCH 18/38] linting --- .github/workflows/branch.yml | 2 +- .github/workflows/linting.yml | 12 ++++++------ .github/workflows/linting_comment.yml | 4 ++-- pyproject.toml | 8 +++++--- 4 files changed, 14 insertions(+), 12 deletions(-) diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 236bc6f6f..3139d7519 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e9..748b43119 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd10c..b706875fc 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c8e..56110621e 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] From e78b791c94fc599177b35f7012f013fa26150033 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 23 Feb 2024 10:45:09 +0100 Subject: [PATCH 19/38] removing tab --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 68e8a4f26..b9c5dbc7d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -904,7 +904,7 @@ process { pattern: 'results_*/*' ] } - + // // SEXDETERRMINE // From e1e8853d4ff12ab29cb50942fd6f1359a0cab932 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 8 Mar 2024 11:48:49 +0100 Subject: [PATCH 20/38] fixing passing ids, bams as list --- conf/modules.config | 4 ++-- subworkflows/local/run_sex_determination.nf | 14 +++++++++++--- 2 files changed, 13 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index b9c5dbc7d..3831eb628 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -910,7 +910,7 @@ process { // withName: SAMTOOLS_DEPTH_SEXDETERMINE { tag = { "${meta1.reference}|${meta1.sample_id}_${meta1.library_id}" } - ext.prefix = { "${meta1.sample_id}_${meta1.library_id}_samtoolsdepth" } + ext.prefix = { "${meta2.id}_samtoolsdepth" } ext.args = '-aa -q30 -Q30 -H' publishDir = [ enabled: false @@ -919,7 +919,7 @@ process { withName: SEXDETERRMINE { tag = { "${meta.reference}|${meta.sample_id}_${meta.library_id}" } - ext.prefix = { "${meta.sample_id}_${meta.library_id}_sexdetermine" } + ext.prefix = { "${meta.reference}_sexdetermine" } publishDir = [ path: { "${params.outdir}/sex_determine/sexdeterrmine/" }, mode: params.publish_dir_mode, diff --git a/subworkflows/local/run_sex_determination.nf b/subworkflows/local/run_sex_determination.nf index 372a2dd03..821c2eb1b 100644 --- a/subworkflows/local/run_sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -31,12 +31,20 @@ workflow RUN_SEXDETERMINE { // Prepend a new meta that contains the meta.reference value as the new_meta.reference attribute WorkflowEager.addNewMetaFromAttributes( it, "reference" , "reference" , false ) } - .combine( ch_bed, by: 0 ) // [ [combine_meta], [meta], bam, bai, [ref_meta], bed ] + .groupTuple(by:0) + //.map { meta, bam, bai -> tuple( groupKey(meta.id)) } + .combine( ch_bed, by: 0 ) // [ [meta], bam, bai, [ref_meta], bed ] + .map { + combo_meta, metas, bam, bai, ref_meta, bed -> + def ids = metas.collect { meta -> meta.id } + [ combo_meta + [id: ids], bam, ref_meta, bed ] // Drop bais + } + //.dump(tag:"1", pretty: true) ch_samtoolsdepth_input = ch_input_sexdetermine .multiMap { - ignore_me, meta, bam, bai, ref_meta, bed -> - bam: [ meta, bam ] + combo_meta, bam, ref_meta, bed -> + bam: [ combo_meta, bam ] bed: [ ref_meta, bed ] } From cd3e744acc360e4927a2bcc757020e22408addc9 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Fri, 8 Mar 2024 11:56:47 +0100 Subject: [PATCH 21/38] linting --- .github/CONTRIBUTING.md | 14 ++++++++------ .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/linting.yml | 2 +- 3 files changed, 10 insertions(+), 8 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 82ae5a145..69e685440 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 2a999dcee..346e387be 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/eage - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/eager/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/eager _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b43119..073e18767 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: From 7d908e0d3a52f8d0bcf142e957d325786a2db0f1 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 09:24:40 +0100 Subject: [PATCH 22/38] Apply suggestions from code review Co-authored-by: Thiseas C. Lamnidis --- subworkflows/local/run_sex_determination.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/subworkflows/local/run_sex_determination.nf b/subworkflows/local/run_sex_determination.nf index 821c2eb1b..c173f1750 100644 --- a/subworkflows/local/run_sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -32,14 +32,12 @@ workflow RUN_SEXDETERMINE { WorkflowEager.addNewMetaFromAttributes( it, "reference" , "reference" , false ) } .groupTuple(by:0) - //.map { meta, bam, bai -> tuple( groupKey(meta.id)) } .combine( ch_bed, by: 0 ) // [ [meta], bam, bai, [ref_meta], bed ] .map { combo_meta, metas, bam, bai, ref_meta, bed -> def ids = metas.collect { meta -> meta.id } [ combo_meta + [id: ids], bam, ref_meta, bed ] // Drop bais } - //.dump(tag:"1", pretty: true) ch_samtoolsdepth_input = ch_input_sexdetermine .multiMap { From 7f6b3cf1480887bc49efc29aa902bd20f1726a26 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 09:45:07 +0100 Subject: [PATCH 23/38] Apply suggestions from code review Co-authored-by: Thiseas C. Lamnidis --- conf/modules.config | 4 ++-- nextflow.config | 2 +- nextflow_schema.json | 2 +- workflows/eager.nf | 2 +- 4 files changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 3831eb628..afc04f3ac 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -919,11 +919,11 @@ process { withName: SEXDETERRMINE { tag = { "${meta.reference}|${meta.sample_id}_${meta.library_id}" } - ext.prefix = { "${meta.reference}_sexdetermine" } + ext.prefix = { "${meta.reference}_sexdeterrmine" } publishDir = [ path: { "${params.outdir}/sex_determine/sexdeterrmine/" }, mode: params.publish_dir_mode, - pattern: '*{_sexdetermine}*', + pattern: '*{_sexdeterrmine}*', enabled: true ] } diff --git a/nextflow.config b/nextflow.config index 58d449e1b..6aa939c88 100644 --- a/nextflow.config +++ b/nextflow.config @@ -204,7 +204,7 @@ params { genotyping_source = 'raw' // Sex determination - run_sexdeterrmine = true + run_sexdeterrmine = false sexdeterrmine_bedfile = null } diff --git a/nextflow_schema.json b/nextflow_schema.json index b009044a5..10a14b062 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1132,7 +1132,7 @@ "properties": { "run_sexdeterrmine": { "type": "boolean", - "default": true, + "default": false, "fa_icon": "fas fa-transgender-alt", "description": "Turn on sex determination for human reference genomes. This will run on single- and double-stranded variants of a library separately.", "help_text": "Specify to run the optional process of sex determination." diff --git a/workflows/eager.nf b/workflows/eager.nf index 4bb5a83e0..7837a1974 100644 --- a/workflows/eager.nf +++ b/workflows/eager.nf @@ -520,7 +520,7 @@ workflow EAGER { if ( params.run_sexdeterrmine ) { ch_sexdeterrmine_input = ch_dedupped_bams - RUN_SEXDETERMINE(ch_sexdeterrmine_input, REFERENCE_INDEXING.out.sexdeterrmine_bed.dump() ) + RUN_SEXDETERMINE(ch_sexdeterrmine_input, REFERENCE_INDEXING.out.sexdeterrmine_bed ) ch_versions = ch_versions.mix( RUN_SEXDETERMINE.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( RUN_SEXDETERMINE.out.mqc.collect{it[1]}.ifEmpty([]) ) } From 3aa9482db3b7110a854c914f5fffa6908450687c Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 09:48:31 +0100 Subject: [PATCH 24/38] aligning comments --- subworkflows/local/run_sex_determination.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/run_sex_determination.nf b/subworkflows/local/run_sex_determination.nf index c173f1750..a811b44c7 100644 --- a/subworkflows/local/run_sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -59,7 +59,7 @@ workflow RUN_SEXDETERMINE { } emit: - coverages = ch_coverages // channel: [ val(meta), path("*.tsv") ] + coverages = ch_coverages // channel: [ val(meta), path("*.tsv") ] mqc = ch_multiqc_files // channel: [ val(meta), path("*.json") ] - versions = ch_versions // channel: path(versions.yml) + versions = ch_versions // channel: path(versions.yml) } From f394152b1f5684846e0f9433ede31e9f9a40d517 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 10:02:46 +0100 Subject: [PATCH 25/38] consistent naming of sexdeterrmine --- conf/modules.config | 4 ++-- subworkflows/local/run_sex_determination.nf | 18 +++++++++--------- workflows/eager.nf | 8 ++++---- 3 files changed, 15 insertions(+), 15 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index afc04f3ac..8bb287347 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -906,9 +906,9 @@ process { } // - // SEXDETERRMINE + // RUN SEXDETERRMINE // - withName: SAMTOOLS_DEPTH_SEXDETERMINE { + withName: SAMTOOLS_DEPTH_SEXDETERRMINE { tag = { "${meta1.reference}|${meta1.sample_id}_${meta1.library_id}" } ext.prefix = { "${meta2.id}_samtoolsdepth" } ext.args = '-aa -q30 -Q30 -H' diff --git a/subworkflows/local/run_sex_determination.nf b/subworkflows/local/run_sex_determination.nf index a811b44c7..f4522bc4b 100644 --- a/subworkflows/local/run_sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -1,11 +1,11 @@ // -// Run sex determine +// Run sex deterrmine // -include { SAMTOOLS_DEPTH as SAMTOOLS_DEPTH_SEXDETERMINE } from '../../modules/nf-core/samtools/depth/main' -include { SEXDETERRMINE } from '../../modules/nf-core/sexdeterrmine/main' +include { SAMTOOLS_DEPTH as SAMTOOLS_DEPTH_SEXDETERRMINE } from '../../modules/nf-core/samtools/depth/main' +include { SEXDETERRMINE } from '../../modules/nf-core/sexdeterrmine/main' -workflow RUN_SEXDETERMINE { +workflow RUN_SEXDETERRMINE { take: // samtoolsdepth_input // channel: [ val(meta1), [ bam ], [ meta2 ] [ intervals_bed ] ] @@ -26,7 +26,7 @@ workflow RUN_SEXDETERMINE { WorkflowEager.addNewMetaFromAttributes( it, "id" , "reference" , false ) } - ch_input_sexdetermine = sexdeterrmine_bam + ch_input_sexdeterrmine = sexdeterrmine_bam .map { // Prepend a new meta that contains the meta.reference value as the new_meta.reference attribute WorkflowEager.addNewMetaFromAttributes( it, "reference" , "reference" , false ) @@ -39,16 +39,16 @@ workflow RUN_SEXDETERMINE { [ combo_meta + [id: ids], bam, ref_meta, bed ] // Drop bais } - ch_samtoolsdepth_input = ch_input_sexdetermine + ch_samtoolsdepth_input = ch_input_sexdeterrmine .multiMap { combo_meta, bam, ref_meta, bed -> bam: [ combo_meta, bam ] bed: [ ref_meta, bed ] } - SAMTOOLS_DEPTH_SEXDETERMINE(ch_samtoolsdepth_input.bam, ch_samtoolsdepth_input.bed) - ch_sex_determine_input = SAMTOOLS_DEPTH_SEXDETERMINE.out.tsv - ch_versions = ch_versions.mix( SAMTOOLS_DEPTH_SEXDETERMINE.out.versions ) + SAMTOOLS_DEPTH_SEXDETERRMINE(ch_samtoolsdepth_input.bam, ch_samtoolsdepth_input.bed) + ch_sex_determine_input = SAMTOOLS_DEPTH_SEXDETERRMINE.out.tsv + ch_versions = ch_versions.mix( SAMTOOLS_DEPTH_SEXDETERRMINE.out.versions ) // Run sex determination with samtools depth input SEXDETERRMINE(ch_sex_determine_input, []) diff --git a/workflows/eager.nf b/workflows/eager.nf index 7837a1974..654e26ea4 100644 --- a/workflows/eager.nf +++ b/workflows/eager.nf @@ -71,7 +71,7 @@ include { MANIPULATE_DAMAGE } from '../subworkflows/local/manipulate include { METAGENOMICS_COMPLEXITYFILTER } from '../subworkflows/local/metagenomics_complexityfilter' include { ESTIMATE_CONTAMINATION } from '../subworkflows/local/estimate_contamination' include { CALCULATE_DAMAGE } from '../subworkflows/local/calculate_damage' -include { RUN_SEXDETERMINE } from '../subworkflows/local/run_sex_determination' +include { RUN_SEXDETERRMINE } from '../subworkflows/local/run_sex_determination' include { MERGE_LIBRARIES } from '../subworkflows/local/merge_libraries' /* @@ -520,9 +520,9 @@ workflow EAGER { if ( params.run_sexdeterrmine ) { ch_sexdeterrmine_input = ch_dedupped_bams - RUN_SEXDETERMINE(ch_sexdeterrmine_input, REFERENCE_INDEXING.out.sexdeterrmine_bed ) - ch_versions = ch_versions.mix( RUN_SEXDETERMINE.out.versions ) - ch_multiqc_files = ch_multiqc_files.mix( RUN_SEXDETERMINE.out.mqc.collect{it[1]}.ifEmpty([]) ) + RUN_SEXDETERRMINE(ch_sexdeterrmine_input, REFERENCE_INDEXING.out.sexdeterrmine_bed ) + ch_versions = ch_versions.mix( RUN_SEXDETERRMINE.out.versions ) + ch_multiqc_files = ch_multiqc_files.mix( RUN_SEXDETERRMINE.out.mqc.collect{it[1]}.ifEmpty([]) ) } // From ef5fd44c592896050e96926cd43e88f18b6e63d7 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 10:06:15 +0100 Subject: [PATCH 26/38] Removing commented lines code --- subworkflows/local/run_sex_determination.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/run_sex_determination.nf b/subworkflows/local/run_sex_determination.nf index f4522bc4b..b4489312b 100644 --- a/subworkflows/local/run_sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -8,7 +8,6 @@ include { SEXDETERRMINE } from '../../modules/n workflow RUN_SEXDETERRMINE { take: - // samtoolsdepth_input // channel: [ val(meta1), [ bam ], [ meta2 ] [ intervals_bed ] ] sexdeterrmine_bam // channel: [ val(meta1), [ bam ] ] sexdeterrmine_bed // channel: [ [ meta2 ] [ intervals_bed ] ] From c9fb55e87886e058275b1f5c7338780d98b9658e Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 10:30:38 +0100 Subject: [PATCH 27/38] Adding manual tests --- conf/modules.config | 2 +- docs/development/manual_tests.md | 13 +++++++++++++ 2 files changed, 14 insertions(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 8bb287347..022cd1f98 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -921,7 +921,7 @@ process { tag = { "${meta.reference}|${meta.sample_id}_${meta.library_id}" } ext.prefix = { "${meta.reference}_sexdeterrmine" } publishDir = [ - path: { "${params.outdir}/sex_determine/sexdeterrmine/" }, + path: { "${params.outdir}/sex_deterrmine/sexdeterrmine/" }, mode: params.publish_dir_mode, pattern: '*{_sexdeterrmine}*', enabled: true diff --git a/docs/development/manual_tests.md b/docs/development/manual_tests.md index 6b5d9ede5..4541c8212 100644 --- a/docs/development/manual_tests.md +++ b/docs/development/manual_tests.md @@ -110,6 +110,12 @@ Tool Specific combinations - single reference: with damage manipulation (pmd + trimming), on pmd filtered data ✅ - multi reference: no damage manipulation ✅ +- Sex determination + + - With sexdeterrmine + + - with default parameters + ### Multi-reference tests ```bash @@ -738,3 +744,10 @@ nextflow run main.nf -profile test,docker --outdir ./results -w work/ -resume -- ## NOTE: PG tags are repeated for each chromosome in the reference, times each library! Maybe there's some flag missing from samtools MERGE runs? nextflow run main.nf -profile test_multiref,docker --outdir ./results -w work/ -resume --genotyping_source 'raw' -ansi-log false -dump-channels ``` + +### Run Sexdeterrmine + +```bash +## Running sex determination subworkflow from deduplicated bams +## Expect: sex_deterrmine/sexdeterrmine directory with tsv summary table for all individuals. +nextflow run main.nf -profile test_humanbam,arm,docker --outdir ./results --run_sexdeterrmine From bc53f26c0092ecf0bb4fe2e85e36c65308d05074 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 10:40:12 +0100 Subject: [PATCH 28/38] Adding sexdeterrmine to CI test --- .github/workflows/ci.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index a1be0195a..818464e66 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -33,6 +33,7 @@ jobs: - "-profile test,docker --mapping_tool bowtie2 --damagecalculation_tool mapdamage --damagecalculation_mapdamage_downsample 100" - "-profile test,docker --skip_preprocessing" - "-profile test_humanbam,docker --run_mtnucratio --run_contamination_estimation_angsd --snpcapture_bed 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz'" + - "-profile test_humanbam,docker --run_sexdeterrmine --sexdeterrmine_bedfile 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz'" - "-profile test_multiref,docker" ## TODO add damage manipulation here instead once it goes multiref steps: - name: Check out pipeline code From ade2cdb3cea9607d4cb5a9a6afe8dc136009bbd3 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 11:36:03 +0100 Subject: [PATCH 29/38] Matching current dev nf-core template --- .github/CONTRIBUTING.md | 14 ++++++-------- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/linting.yml | 10 +++++----- .github/workflows/linting_comment.yml | 4 ++-- pyproject.toml | 8 +++----- 6 files changed, 18 insertions(+), 22 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 69e685440..82ae5a145 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,8 +9,9 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> [!NOTE] -> If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). +:::info +If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). +::: ## Contribution workflow @@ -26,11 +27,8 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: - -```bash -nf-test test --profile debug,test,docker --verbose -``` +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -92,7 +90,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 346e387be..2a999dcee 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/eage - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/eager/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/eager _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 3139d7519..236bc6f6f 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e18767..81cd098e9 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@v4 - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + uses: actions/setup-python@v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index b706875fc..147bcd10c 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 + uses: marocchino/sticky-pull-request-comment@v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/pyproject.toml b/pyproject.toml index 56110621e..7d08e1c8e 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,13 +3,11 @@ [tool.ruff] line-length = 120 target-version = "py38" -cache-dir = "~/.cache/ruff" - -[tool.ruff.lint] select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.ruff.lint.isort] +[tool.ruff.isort] known-first-party = ["nf_core"] -[tool.ruff.lint.per-file-ignores] +[tool.ruff.per-file-ignores] "__init__.py" = ["E402", "F401"] From 8a01c0cd06f90ac10556d5ca01d634ff72b1ac6b Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 12:50:46 +0100 Subject: [PATCH 30/38] Apply suggestions from code review Co-authored-by: Thiseas C. Lamnidis --- .github/workflows/ci.yml | 2 +- conf/modules.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 818464e66..edcba0b80 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -33,7 +33,7 @@ jobs: - "-profile test,docker --mapping_tool bowtie2 --damagecalculation_tool mapdamage --damagecalculation_mapdamage_downsample 100" - "-profile test,docker --skip_preprocessing" - "-profile test_humanbam,docker --run_mtnucratio --run_contamination_estimation_angsd --snpcapture_bed 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz'" - - "-profile test_humanbam,docker --run_sexdeterrmine --sexdeterrmine_bedfile 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/reference/Human/1240K.pos.list_hs37d5.0based.bed.gz'" + - "-profile test_humanbam,docker --run_sexdeterrmine" - "-profile test_multiref,docker" ## TODO add damage manipulation here instead once it goes multiref steps: - name: Check out pipeline code diff --git a/conf/modules.config b/conf/modules.config index 022cd1f98..b9d23719f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -921,7 +921,7 @@ process { tag = { "${meta.reference}|${meta.sample_id}_${meta.library_id}" } ext.prefix = { "${meta.reference}_sexdeterrmine" } publishDir = [ - path: { "${params.outdir}/sex_deterrmine/sexdeterrmine/" }, + path: { "${params.outdir}/sex_determination/" }, mode: params.publish_dir_mode, pattern: '*{_sexdeterrmine}*', enabled: true From 384bc9039927298010fabc044cbed81ee5dfcecf Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Mon, 18 Mar 2024 12:57:40 +0100 Subject: [PATCH 31/38] prettier --- docs/development/manual_tests.md | 1 + 1 file changed, 1 insertion(+) diff --git a/docs/development/manual_tests.md b/docs/development/manual_tests.md index 4541c8212..4539c0899 100644 --- a/docs/development/manual_tests.md +++ b/docs/development/manual_tests.md @@ -751,3 +751,4 @@ nextflow run main.nf -profile test_multiref,docker --outdir ./results -w work/ - ## Running sex determination subworkflow from deduplicated bams ## Expect: sex_deterrmine/sexdeterrmine directory with tsv summary table for all individuals. nextflow run main.nf -profile test_humanbam,arm,docker --outdir ./results --run_sexdeterrmine +``` From cabc09e688bf1bfa17a72e9cb2e53f8e565e4596 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Wed, 20 Mar 2024 09:34:43 +0100 Subject: [PATCH 32/38] including "addNewMetaFromAttributes" --- modules.json | 19 ---- .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 24 +++++ .../custom/dumpsoftwareversions/meta.yml | 37 +++++++ .../templates/dumpsoftwareversions.py | 101 ++++++++++++++++++ subworkflows/local/run_sex_determination.nf | 6 +- 6 files changed, 173 insertions(+), 21 deletions(-) create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf create mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml create mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules.json b/modules.json index f27509d18..afa4336fc 100644 --- a/modules.json +++ b/modules.json @@ -227,25 +227,6 @@ } } }, - "subworkflows": { - "nf-core": { - "bam_docounts_contamination_angsd": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - }, - "bam_split_by_region": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - }, - "fastq_align_bwaaln": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - } - } - }, "subworkflows": { "nf-core": { "utils_nextflow_pipeline": { diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 000000000..9b3272bc1 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf new file mode 100644 index 000000000..f2187611c --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -0,0 +1,24 @@ +process CUSTOM_DUMPSOFTWAREVERSIONS { + label 'process_single' + + // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + + input: + path versions + + output: + path "software_versions.yml" , emit: yml + path "software_versions_mqc.yml", emit: mqc_yml + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + template 'dumpsoftwareversions.py' +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml new file mode 100644 index 000000000..5f15a5fde --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -0,0 +1,37 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: custom_dumpsoftwareversions +description: Custom module used to dump software versions within the nf-core pipeline template +keywords: + - custom + - dump + - version +tools: + - custom: + description: Custom module used to dump software versions within the nf-core pipeline template + homepage: https://github.com/nf-core/tools + documentation: https://github.com/nf-core/tools + licence: ["MIT"] +input: + - versions: + type: file + description: YML file containing software versions + pattern: "*.yml" +output: + - yml: + type: file + description: Standard YML file containing software versions + pattern: "software_versions.yml" + - mqc_yml: + type: file + description: MultiQC custom content YML file containing software versions + pattern: "software_versions_mqc.yml" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py new file mode 100755 index 000000000..da0334085 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -0,0 +1,101 @@ +#!/usr/bin/env python + + +"""Provide functions to merge multiple versions.yml files.""" + + +import yaml +import platform +from textwrap import dedent + + +def _make_versions_html(versions): + """Generate a tabular HTML output of all versions for MultiQC.""" + html = [ + dedent( + """\\ + + + + + + + + + + """ + ) + ] + for process, tmp_versions in sorted(versions.items()): + html.append("") + for i, (tool, version) in enumerate(sorted(tmp_versions.items())): + html.append( + dedent( + f"""\\ + + + + + + """ + ) + ) + html.append("") + html.append("
Process Name Software Version
{process if (i == 0) else ''}{tool}{version}
") + return "\\n".join(html) + + +def main(): + """Load all version files and generate merged output.""" + versions_this_module = {} + versions_this_module["${task.process}"] = { + "python": platform.python_version(), + "yaml": yaml.__version__, + } + + with open("$versions") as f: + versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + + # aggregate versions by the module name (derived from fully-qualified process name) + versions_by_module = {} + for process, process_versions in versions_by_process.items(): + module = process.split(":")[-1] + try: + if versions_by_module[module] != process_versions: + raise AssertionError( + "We assume that software versions are the same between all modules. " + "If you see this error-message it means you discovered an edge-case " + "and should open an issue in nf-core/tools. " + ) + except KeyError: + versions_by_module[module] = process_versions + + versions_by_module["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version", + } + + versions_mqc = { + "id": "software_versions", + "section_name": "${workflow.manifest.name} Software Versions", + "section_href": "https://github.com/${workflow.manifest.name}", + "plot_type": "html", + "description": "are collected at run time from the software output.", + "data": _make_versions_html(versions_by_module), + } + + with open("software_versions.yml", "w") as f: + yaml.dump(versions_by_module, f, default_flow_style=False) + with open("software_versions_mqc.yml", "w") as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + + with open("versions.yml", "w") as f: + yaml.dump(versions_this_module, f, default_flow_style=False) + + +if __name__ == "__main__": + main() diff --git a/subworkflows/local/run_sex_determination.nf b/subworkflows/local/run_sex_determination.nf index b4489312b..8917c1575 100644 --- a/subworkflows/local/run_sex_determination.nf +++ b/subworkflows/local/run_sex_determination.nf @@ -2,6 +2,8 @@ // Run sex deterrmine // +include { addNewMetaFromAttributes } from '../../subworkflows/local/utils_nfcore_eager_pipeline/main' + include { SAMTOOLS_DEPTH as SAMTOOLS_DEPTH_SEXDETERRMINE } from '../../modules/nf-core/samtools/depth/main' include { SEXDETERRMINE } from '../../modules/nf-core/sexdeterrmine/main' @@ -22,13 +24,13 @@ workflow RUN_SEXDETERRMINE { ch_bed = sexdeterrmine_bed .map { // Prepend a new meta that contains the meta.id value as the new_meta.reference attribute - WorkflowEager.addNewMetaFromAttributes( it, "id" , "reference" , false ) + addNewMetaFromAttributes( it, "id" , "reference" , false ) } ch_input_sexdeterrmine = sexdeterrmine_bam .map { // Prepend a new meta that contains the meta.reference value as the new_meta.reference attribute - WorkflowEager.addNewMetaFromAttributes( it, "reference" , "reference" , false ) + addNewMetaFromAttributes( it, "reference" , "reference" , false ) } .groupTuple(by:0) .combine( ch_bed, by: 0 ) // [ [meta], bam, bai, [ref_meta], bed ] From ddb4d78f30dd94579a755e84d335362099d84ec0 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Wed, 20 Mar 2024 10:15:30 +0100 Subject: [PATCH 33/38] removing subworflows --- modules.json | 19 ------------------- 1 file changed, 19 deletions(-) diff --git a/modules.json b/modules.json index afa4336fc..a09025e9d 100644 --- a/modules.json +++ b/modules.json @@ -226,25 +226,6 @@ "installed_by": ["modules"] } } - }, - "subworkflows": { - "nf-core": { - "utils_nextflow_pipeline": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - }, - "utils_nfcore_pipeline": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - }, - "utils_nfvalidation_plugin": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - } - } } } } From 6ebc965f64f2b343762861f8be8c7754acbca50e Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Wed, 20 Mar 2024 10:17:50 +0100 Subject: [PATCH 34/38] lint --- .github/CONTRIBUTING.md | 10 +- .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 3 +- .github/workflows/branch.yml | 2 +- .github/workflows/linting.yml | 67 +++++++++--- .github/workflows/linting_comment.yml | 4 +- CODE_OF_CONDUCT.md | 133 ++++++----------------- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-eager_logo_light.png | Bin 71836 -> 10462 bytes docs/images/nf-core-eager_logo_dark.png | Bin 26486 -> 68937 bytes docs/images/nf-core-eager_logo_light.png | Bin 22876 -> 68825 bytes pyproject.toml | 19 ++-- 13 files changed, 100 insertions(+), 144 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 69e685440..4edb21605 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,7 +9,6 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> [!NOTE] > If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -26,12 +25,6 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: - -```bash -nf-test test --profile debug,test,docker --verbose -``` - When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -92,7 +85,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. ### Naming schemes @@ -123,3 +116,4 @@ To get started: Devcontainer specs: - [DevContainer config](.devcontainer/devcontainer.json) +- [Dockerfile](.devcontainer/Dockerfile) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 46e4febcc..013769de0 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -42,7 +42,7 @@ body: attributes: label: System information description: | - * Nextflow version _(eg. 23.04.0)_ + * Nextflow version _(eg. 22.10.1)_ * Hardware _(eg. HPC, Desktop, Cloud)_ * Executor _(eg. slurm, local, awsbatch)_ * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_ diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 346e387be..55768a0c1 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,8 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/eage - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/eager/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/eager _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). -- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 3139d7519..00f205457 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 + uses: mshick/add-pr-comment@v1 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e18767..888cb4bc3 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,35 +11,74 @@ on: types: [published] jobs: - pre-commit: + EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@v3 - - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-node@v3 + + - name: Install editorconfig-checker + run: npm install -g editorconfig-checker + + - name: Run ECLint check + run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') + + Prettier: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - uses: actions/setup-node@v3 + + - name: Install Prettier + run: npm install -g prettier + + - name: Run Prettier --check + run: prettier --check ${GITHUB_WORKSPACE} + + PythonBlack: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - name: Check code lints with Black + uses: psf/black@stable + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 with: - python-version: 3.11 - cache: "pip" + message: | + ## Python linting (`black`) is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` + * Fix formatting errors in your pipeline: `black .` + + Once you push these changes the test should pass, and you can hide this comment :+1: - - name: Install pre-commit - run: pip install pre-commit + We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! - - name: Run pre-commit - run: pre-commit run --all-files + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@v3 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@v4 with: - python-version: "3.11" + python-version: "3.8" architecture: "x64" - name: Install dependencies @@ -60,7 +99,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@v3 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index b706875fc..0bbcd30f2 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 + uses: dawidd6/action-download-artifact@v2 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 + uses: marocchino/sticky-pull-request-comment@v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index c089ec78c..f4fd052f1 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,20 +1,18 @@ -# Code of Conduct at nf-core (v1.4) +# Code of Conduct at nf-core (v1.0) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age -- Ability - Body size -- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Neurodiversity +- Physical and neurological ability - Race or ethnicity - Religion - Sexual identity and orientation @@ -24,133 +22,80 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -:::note -This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. -::: +> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). - -Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. +We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. +Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re ## Our Responsibilities -Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. +Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. -## When and where does this Code of Conduct apply? +## When are where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Accept feedback, yet be unafraid to question, deliberate, and learn. +- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) - Take breaks when you feel like you need them. -- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) +- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. +- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. -All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. +All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for reporting CoC violations +## Procedures for Reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. - -Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. - -All reports will be handled with the utmost discretion and confidentiality. - -You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: - -- Your contact information. -- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. -- The behaviour that was in violation and the circumstances surrounding the incident. -- The approximate time of the behaviour (if different than the time the report was made). -- Other people involved in the incident, if applicable. -- If you believe the incident is ongoing. -- If there is a publicly available record (e.g. mailing list record, a screenshot). -- Any additional information. - -After you file a report, one or more members of our Safety Team will contact you to follow up on your report. - -## Who will read and handle reports - -All reports will be read and handled by the members of the Safety Team at nf-core. - -If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. - -To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. - -## Reviewing reports - -After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. - -The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. - -In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. +You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). -Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. +Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. -## Confidentiality - -All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. - -We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. - -## Enforcement - -Actions taken by the nf-core’s Safety Team may include, but are not limited to: - -- Asking anyone to stop a behaviour. -- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. -- Removing access to the gather.town and Slack, either temporarily or permanently. -- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. -- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. -- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. -- No action. +All reports will be handled with utmost discretion and confidentially. ## Attribution and Acknowledgements @@ -161,22 +106,6 @@ Actions taken by the nf-core’s Safety Team may include, but are not limited to ## Changelog -### v1.4 - February 8th, 2022 - -- Included a new member of the Safety Team. Corrected a typographical error in the text. - -### v1.3 - December 10th, 2021 - -- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. - -### v1.2 - November 12th, 2021 - -- Removed information specific to reporting CoC violations at the Hackathon in October 2021. - -### v1.1 - October 14th, 2021 - -- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. - -### v1.0 - March 15th, 2021 +### v1.0 - March 12th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/assets/email_template.html b/assets/email_template.html index 2b66b754d..36bfc9c8d 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/eager ${version}

+

nf-core/eager v${version}

Run Name: $runName

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z!i@la%0W(FOE5cgC@Rk>w=Z~$?p`9JD3b^oLa^dc&hMmlE+~xDeiWnvge7H<(7`Y1 zcA~2Nd-wo~M7HEYD_j*xq)!J8kS$J7{r-(4gsM1&g)l@+xFGAX Date: Wed, 20 Mar 2024 10:42:02 +0100 Subject: [PATCH 35/38] lint --- modules.json | 62 ++++++++++++++++++++++++++++++++++++++++------------ 1 file changed, 48 insertions(+), 14 deletions(-) diff --git a/modules.json b/modules.json index a09025e9d..06301c2a0 100644 --- a/modules.json +++ b/modules.json @@ -52,27 +52,27 @@ }, "bwa/aln": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "6278bf9afd4a4b2d00fa6052250e73da3d91546f", "installed_by": ["fastq_align_bwaaln"] }, "bwa/index": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "6278bf9afd4a4b2d00fa6052250e73da3d91546f", "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "1e2b7fb7106852388610c0360d234b0829eb980e", "installed_by": ["modules"] }, "bwa/sampe": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "1e2b7fb7106852388610c0360d234b0829eb980e", "installed_by": ["fastq_align_bwaaln"] }, "bwa/samse": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "1e2b7fb7106852388610c0360d234b0829eb980e", "installed_by": ["fastq_align_bwaaln"] }, "cat/fastq": { @@ -107,7 +107,7 @@ }, "fastp": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56", "installed_by": ["modules"] }, "fastqc": { @@ -182,38 +182,38 @@ }, "samtools/fastq": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", + "git_sha": "8d8f0ae52d6c9342bd41c33dda0b74b07e32153d", "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", + "git_sha": "8d8f0ae52d6c9342bd41c33dda0b74b07e32153d", "installed_by": ["modules"] }, "samtools/idxstats": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", + "git_sha": "8d8f0ae52d6c9342bd41c33dda0b74b07e32153d", "installed_by": ["modules"] }, "samtools/index": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", + "git_sha": "8d8f0ae52d6c9342bd41c33dda0b74b07e32153d", "installed_by": ["bam_split_by_region", "fastq_align_bwaaln"] }, "samtools/merge": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", + "git_sha": "8d8f0ae52d6c9342bd41c33dda0b74b07e32153d", "installed_by": ["modules"] }, "samtools/sort": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", + "git_sha": "8d8f0ae52d6c9342bd41c33dda0b74b07e32153d", "installed_by": ["modules"] }, "samtools/view": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_split_by_region", "modules"] + "git_sha": "8d8f0ae52d6c9342bd41c33dda0b74b07e32153d", + "installed_by": ["bam_split_by_region"] }, "seqkit/split2": { "branch": "master", @@ -226,6 +226,40 @@ "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "bam_docounts_contamination_angsd": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + }, + "bam_split_by_region": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + }, + "fastq_align_bwaaln": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + }, + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + } + } } } } From d03589b5bcf6061c20a1d17b002ad2fdcd6acb00 Mon Sep 17 00:00:00 2001 From: Judith Ballesteros Date: Wed, 20 Mar 2024 10:44:27 +0100 Subject: [PATCH 36/38] fix linting --- .github/CONTRIBUTING.md | 10 +- .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 3 +- .github/workflows/branch.yml | 2 +- .github/workflows/linting.yml | 67 +++--------- .github/workflows/linting_comment.yml | 4 +- CODE_OF_CONDUCT.md | 133 +++++++++++++++++------ assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-eager_logo_light.png | Bin 10462 -> 71836 bytes docs/images/nf-core-eager_logo_dark.png | Bin 68937 -> 26486 bytes docs/images/nf-core-eager_logo_light.png | Bin 68825 -> 22876 bytes pyproject.toml | 19 ++-- 13 files changed, 144 insertions(+), 100 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 4edb21605..69e685440 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,6 +9,7 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) +> [!NOTE] > If you need help using or modifying nf-core/eager then the best place to ask is on the nf-core Slack [#eager](https://nfcore.slack.com/channels/eager) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -25,6 +26,12 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -85,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes @@ -116,4 +123,3 @@ To get started: Devcontainer specs: - [DevContainer config](.devcontainer/devcontainer.json) -- [Dockerfile](.devcontainer/Dockerfile) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 013769de0..46e4febcc 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -42,7 +42,7 @@ body: attributes: label: System information description: | - * Nextflow version _(eg. 22.10.1)_ + * Nextflow version _(eg. 23.04.0)_ * Hardware _(eg. HPC, Desktop, Cloud)_ * Executor _(eg. slurm, local, awsbatch)_ * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_ diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 55768a0c1..346e387be 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,8 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/eage - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/eager/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/eager _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 00f205457..3139d7519 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 888cb4bc3..073e18767 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,74 +11,35 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - - uses: actions/setup-node@v3 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - uses: actions/setup-node@v3 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: - python-version: "3.8" + python-version: "3.11" architecture: "x64" - name: Install dependencies @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f2..b706875fc 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index f4fd052f1..c089ec78c 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,18 +1,20 @@ -# Code of Conduct at nf-core (v1.0) +# Code of Conduct at nf-core (v1.4) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age +- Ability - Body size +- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Physical and neurological ability +- Neurodiversity - Race or ethnicity - Religion - Sexual identity and orientation @@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. ## Our Responsibilities -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. -## When are where does this Code of Conduct apply? +## When and where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Accept feedback, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) - Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for Reporting CoC violations +## Procedures for reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. -All reports will be handled with utmost discretion and confidentially. +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. ## Attribution and Acknowledgements @@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially. ## Changelog -### v1.0 - March 12th, 2021 +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/assets/email_template.html b/assets/email_template.html index 36bfc9c8d..2b66b754d 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

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+

nf-core/eager ${version}

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z=dLlysZ@Ko+E%=Hu8{Y3H@7gIl%bF$A6Q9wIL0w0^Drldmm{|*WjD&Kv0Mb8P4UT5Gt)XQXYcNlz! zPtd9YvKQTOXitTlz?@a*wtzmINUfY3zCeZ2|Gnif@FcMMPUG(-bKM;$&)cY^GR9@(VHW}_b4gZRlWl=48R>X}TXQs^*YFV-!H}jI z`}!%y?a6a$t{@XxZud6=E~&zF@&@TM$P;%!&ScUYmUu9j%&%vTFwtoD>(K}Pv9Yhi zwRCbSpAhYvyN^xI0`H{|^X=;ErE67ibn<^(_PeP#+IaFivt7R z-+#}wz8Gp}>&59I%ZB#fqE7BbQ#;9_pY7pb6e+6}gv!*!ShSQ1tj31>D}v~+398W! zI2XvF6<269&EEUQi!|kMJ}ha8EVRLRQs}ziXBSi3h|qFf+J(N4izB#4XyO}qa_{3U zs$Nh`Ray$yGM#5nBN5jOw8BrY7#-BriCm%oZS2^d_mbmAY{ko8uFT%QaWf2TQ0}k% z2Od+^G1sP_b~`vYiW8A0eXSVfi()86(@{txnzbu##Ve+>@6JAT6KW$A3K^q^eLY=} zD|rw39C|IhUxn^|;vukuU_qhplpE3(ynOZh*8j{P4i+pZ@7 diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6f..56110621e 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,15 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] +known-first-party = ["nf_core"] + +[tool.ruff.lint.per-file-ignores] +"__init__.py" = ["E402", "F401"] From f66e0fd9a04fe954be68e587f0b8a989eed1cbac Mon Sep 17 00:00:00 2001 From: Thiseas Christos Lamnidis Date: Wed, 20 Mar 2024 11:35:50 +0100 Subject: [PATCH 37/38] remove dumpsoftwareversions. linting --- modules.json | 8 +- .../dumpsoftwareversions/environment.yml | 7 -- .../custom/dumpsoftwareversions/main.nf | 24 ----- .../custom/dumpsoftwareversions/meta.yml | 37 ------- .../templates/dumpsoftwareversions.py | 101 ------------------ 5 files changed, 4 insertions(+), 173 deletions(-) delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules.json b/modules.json index de7cf7e46..1b9488483 100644 --- a/modules.json +++ b/modules.json @@ -260,14 +260,14 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "sexdeterrmine": { + "sequencetools/pileupcaller": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "sequencetools/pileupcaller": { + "sexdeterrmine": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc1..000000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611c..000000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fde..000000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index da0334085..000000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,101 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import yaml -import platform -from textwrap import dedent - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - - - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
Process Name Software Version
{process if (i == 0) else ''}{tool}{version}
") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() From 624ba695422920e66e3b6113e424181ad62977c9 Mon Sep 17 00:00:00 2001 From: "Thiseas C. Lamnidis" Date: Wed, 20 Mar 2024 11:38:28 +0100 Subject: [PATCH 38/38] Update nextflow.config --- nextflow.config | 1 - 1 file changed, 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 834fbd9cd..5865a2943 100644 --- a/nextflow.config +++ b/nextflow.config @@ -205,7 +205,6 @@ params { sexdeterrmine_bedfile = null // Genotyping - genotyping_source = 'raw' run_genotyping = false genotyping_tool = null genotyping_source = null