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When I used this software to trim amplicon reads for 515F-806R primer, it reported the error as follows:
ERROR: Unexpected character 'Y' found. Program execution aborted.
the following are the shell command and the primer sequance:
flexbar -a illumina_PE_adapter.fa \ -ao 5 \ -qt 30 \ --min-read-length 180 \ --threads 2 \ --zip-output GZ \ --max-uncalled 10 \ -r /disk/user/zouhua/project/Hypertension_16s/Study/00.RawData/HTN1/FDMP20H002931-1a_L1_HTN1.raw_1.fq.gz \ -p /disk/user/zouhua/project/Hypertension_16s/Study/00.RawData/HTN1/FDMP20H002931-1a_L1_HTN1.raw_2.fq.gz \ --target result/02.trim/HTN1 \ 2>result/logs/02.trim/trim_HTN1.log
>forward_sequence_515F CCTAYGGGRBGCASCAG >reverse_sequence_806R GGACTACNNGGGTATCTAAT
So I wonder that whether flexbar is suitable on trimming primer sequence ?
The text was updated successfully, but these errors were encountered:
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When I used this software to trim amplicon reads for 515F-806R primer, it reported the error as follows:
the following are the shell command and the primer sequance:
flexbar -a illumina_PE_adapter.fa \ -ao 5 \ -qt 30 \ --min-read-length 180 \ --threads 2 \ --zip-output GZ \ --max-uncalled 10 \ -r /disk/user/zouhua/project/Hypertension_16s/Study/00.RawData/HTN1/FDMP20H002931-1a_L1_HTN1.raw_1.fq.gz \ -p /disk/user/zouhua/project/Hypertension_16s/Study/00.RawData/HTN1/FDMP20H002931-1a_L1_HTN1.raw_2.fq.gz \ --target result/02.trim/HTN1 \ 2>result/logs/02.trim/trim_HTN1.log
So I wonder that whether flexbar is suitable on trimming primer sequence ?
The text was updated successfully, but these errors were encountered: