Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

UMI tags can't be add to reads name properly while pair end mode #37

Open
soappp9527 opened this issue Jul 21, 2022 · 0 comments
Open

Comments

@soappp9527
Copy link

Thanks for your brilliant work! flexbar is my most used trimming tool. But I found a little defect recently when enabling --umi-tags. The UMI would be added behind reads number but reads name, like this @E100047096L1C001R0030000415/2_TGCACGAGAC. So I need to use some tools like bioawk to relocate the UMI tags otherwise they will be ignored by mapping tools or cause the error. Maybe you can add some identify pattern options and let UMI tags add correctly?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant