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While analyzing sequencing data biologists sent me, I found quite a big issue with the F/R barcodes combinations.
For a fastq file, they provide two barcodes fasta file (F and R), both with 7 barcodes. The problem is that flexbar (used with the -b and -b2 parameters) seems to search for all possible F/R barcodes combinations (thus 49), while they used less combinations (19 in total).
My question is : is it possible to exclude barcodes combinations (or to specify which one to use) ?
Thanks.
The text was updated successfully, but these errors were encountered:
Hello,
While analyzing sequencing data biologists sent me, I found quite a big issue with the F/R barcodes combinations.
For a fastq file, they provide two barcodes fasta file (F and R), both with 7 barcodes. The problem is that flexbar (used with the -b and -b2 parameters) seems to search for all possible F/R barcodes combinations (thus 49), while they used less combinations (19 in total).
My question is : is it possible to exclude barcodes combinations (or to specify which one to use) ?
Thanks.
The text was updated successfully, but these errors were encountered: