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Exclude some barcodes combinations ? #43

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Mataivic opened this issue Mar 24, 2023 · 0 comments
Open

Exclude some barcodes combinations ? #43

Mataivic opened this issue Mar 24, 2023 · 0 comments

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@Mataivic
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Hello,

While analyzing sequencing data biologists sent me, I found quite a big issue with the F/R barcodes combinations.

For a fastq file, they provide two barcodes fasta file (F and R), both with 7 barcodes. The problem is that flexbar (used with the -b and -b2 parameters) seems to search for all possible F/R barcodes combinations (thus 49), while they used less combinations (19 in total).

My question is : is it possible to exclude barcodes combinations (or to specify which one to use) ?

Thanks.

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