From 13f240a710e5802b63ca6ae1523f34b7f68df931 Mon Sep 17 00:00:00 2001 From: Lydia Buntrock Date: Tue, 3 Aug 2021 18:20:56 +0200 Subject: [PATCH] [TEST] Add more test cases to mini example: Translocation and Duplication Signed-off-by: Lydia Buntrock --- test/cli/iGenVar_cli_test.cpp | 3 +- test/data/datasources.cmake | 6 ++-- test/data/mini_example/explanation.fasta | 26 ++++++++++-------- .../mini_example/mini_example_reference.fasta | 4 ++- test/data/mini_example/output_err.txt | 11 +++++++- test/data/mini_example/output_res.txt | 4 ++- .../data/mini_example/single_end_IGV_View.png | Bin 81792 -> 112731 bytes .../mini_example/single_end_mini_example.sam | 14 +++++++++- 8 files changed, 49 insertions(+), 19 deletions(-) diff --git a/test/cli/iGenVar_cli_test.cpp b/test/cli/iGenVar_cli_test.cpp index 8af6dafd..9836aabf 100644 --- a/test/cli/iGenVar_cli_test.cpp +++ b/test/cli/iGenVar_cli_test.cpp @@ -526,7 +526,8 @@ TEST_F(iGenVar_cli_test, dataset_single_end_mini_example) { cli_test_result result = execute_app("iGenVar", "-j", data("single_end_mini_example.sam"), - "--method cigar_string --method split_read --min_var_length 8"); + "--method cigar_string --method split_read " + "--min_var_length 8 --max_var_length 400"); // Check the output of junctions: seqan3::debug_stream << "Check the output of junctions... " << '\n'; diff --git a/test/data/datasources.cmake b/test/data/datasources.cmake index f775de52..059d9c4c 100644 --- a/test/data/datasources.cmake +++ b/test/data/datasources.cmake @@ -19,14 +19,14 @@ declare_datasource (FILE paired_end_mini_example.sam # copies file to /data/single_end_mini_example.sam declare_datasource (FILE single_end_mini_example.sam URL ${CMAKE_SOURCE_DIR}/test/data/mini_example/single_end_mini_example.sam - URL_HASH SHA256=ea89fb00c802e5136ca8dd32362648c8192652a34078bfa25b8ae78695a27cd6) + URL_HASH SHA256=41f4591dd511406e5c3a79bf8e07af58110d03ad327592e4d8d6bdd8cbea7e10) # copies file to /data/output_err.txt declare_datasource (FILE output_err.txt URL ${CMAKE_SOURCE_DIR}/test/data/mini_example/output_err.txt - URL_HASH SHA256=415171afbdf18cffef58599af346b73208457f4406343cf5e69a1d125b4a9c02) + URL_HASH SHA256=5f2c89eb3aa4838e7c4ff9b2d08d0d715064f5e42e6851ecaddef4156e8e4655) # copies file to /data/output_res.txt declare_datasource (FILE output_res.txt URL ${CMAKE_SOURCE_DIR}/test/data/mini_example/output_res.txt - URL_HASH SHA256=1b9e3c3f2e7d599b50370bdfb16d41d485c5efdf5ceb2b63e17fc69c40ec71dc) + URL_HASH SHA256=a067034e05e8a39f3381c9cd2594d10fc8ccd74cf87069ab67ceb54de70b1693) diff --git a/test/data/mini_example/explanation.fasta b/test/data/mini_example/explanation.fasta index a11ea1fb..14825f08 100644 --- a/test/data/mini_example/explanation.fasta +++ b/test/data/mini_example/explanation.fasta @@ -1,25 +1,29 @@ >ref -CGCCCATGCAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCG +CGCCCATGCA ACTAGCGATGCTAGCTAGCTAGCT TACGACTGGCCATGCG GCGGCAT ATTTATAACGGGC ATACATAAGGGGCTCATCGATCGATTTCGG ATCGGGGGGCCCCCATTTTAAACGG GGATCTTGACTCTGGAAAACTTTTA ACGCCGGGAATCGGTAGTCCTTTCGCGGGG ATATATTT TACGGGATATAA CGATCGGATCGGATCGATAATAGGTCTCGGTTGCCAACTGATCGTACCAA ATATTTCTGCGGGGCT CGCCCCTCCGCGATTA AGAG TCGGCTAACGGTT C ACCCATGCGGATTATAAACGGG ATATTAAGGGCTTT ATATTAAGGGCTTT -AGGGCTAGCTAGGTCCAA +AGGGCTAGCTAGGTCCAA AGCTTTCGGAACGTAC GGTAACGTGTA CGGTA +CGTACTGAAAAAAACCAAACGGGGAAATATTAACCGGCCTTTGCCTGGGA +CGCTGTGACTGTACGGGGGGGCGGGCCTTATT >new_Creature -CGCCCATGCAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCG breakpoints: -GCGGCAT ATACATAAGGGGCTCATCGATCGATTTCGG <- Deletion ref: [57, 70] deleted -ATCGGGGGGCCCCCATTTTAAACGG CCCCGGGGCCAATTT GGATCTTGACTCTGGAAAACTTTTA <- Insertion ref: after 125 something inserted -ACGCCGGGAATCGGTAGTCCTTTCGCGGGG ATATATTT ATATATTT TACGGGATATAA <- Duplication ref: (180 or 189) something inserted +CGCCCATGCAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCG breakpoints: +GCGGCAT ATACATAAGGGGCTCATCGATCGATTTCGG <- Deletion ref: (57, 70] deleted +ATCGGGGGGCCCCCATTTTAAACGG CCCCGGGGCCAATTT GGATCTTGACTCTGGAAAACTTTTA <- Insertion ref: novel element inserted after 125 +ACGCCGGGAATCGGTAGTCCTTTCGCGGGG ATATATTT ATATATTT TACGGGATATAA <- Tandem Duplication ref: (180, 188] tandem duplicated CGATCGGATCGGATCGATAATAGGTCTCGGTTGCCAACTGATCGTACCAA -ATATTTCTGCGGGGCT TCGGCTAACGGTT AGAG CGCCCCTCCGCGATTA C <- Translocation ref: (266, 267, 232, 233, 236, 237, 249) - oder ref: [267, 286] deleted -ACCCATGCGGATTATAAACGGG ATATTAAGGGCTTT <- Duplication in der Referenz -AGGGCTAGCTAGGTCCAA +ATATTTCTGCGGGGCT TCGGCTAACGGTT AGAG CGCCCCTCCGCGATTA C <- Double Translocation ref: (266, 282] translocated after 286 + or ref: (286, 299] translocated after 266 +ACCCATGCGGATTATAAACGGG ATATTAAGGGCTTT <- Duplication in the ref: maps to (322, 336] & (336, 350] +AGGGCTAGCTAGGTCCAA GGTAACGTGTA AGCTTTCGGAACGTAC CGGTA <- Translocation ref: (368, 384] translocated after 395 +CGTACTGAAAAAAACCAAACGGGGAAATATTAACCGGCCTTTGCCTGGGA +CGCTGTGACTGTACGGGGGGG ACTAGCGATGCTAGCTAGCTAGCT CGGGCCTTATT <- Duplication ref: (10, 34] duplicated after 471 ->insertion +>novel_element_insertion CCCCGGGGCCAATTT 2048: split reads diff --git a/test/data/mini_example/mini_example_reference.fasta b/test/data/mini_example/mini_example_reference.fasta index 817322b7..193fe96d 100644 --- a/test/data/mini_example/mini_example_reference.fasta +++ b/test/data/mini_example/mini_example_reference.fasta @@ -6,4 +6,6 @@ ACGCCGGGAATCGGTAGTCCTTTCGCGGGGATATATTTTACGGGATATAA CGATCGGATCGGATCGATAATAGGTCTCGGTTGCCAACTGATCGTACCAA ATATTTCTGCGGGGCTCGCCCCTCCGCGATTAAGAGTCGGCTAACGGTTC ACCCATGCGGATTATAAACGGGATATTAAGGGCTTTATATTAAGGGCTTT -AGGGCTAGCTAGGTCCAA +AGGGCTAGCTAGGTCCAAAGCTTTCGGAACGTACGGTAACGTGTACGGTA +CGTACTGAAAAAAACCAAACGGGGAAATATTAACCGGCCTTTGCCTGGGA +CGCTGTGACTGTACGGGGGGGCGGGCCTTATT diff --git a/test/data/mini_example/output_err.txt b/test/data/mini_example/output_err.txt index c437d0dd..13533384 100644 --- a/test/data/mini_example/output_err.txt +++ b/test/data/mini_example/output_err.txt @@ -33,6 +33,15 @@ DEL: chr1 335 Forward chr1 350 Forward 0 read042 DEL: chr1 335 Forward chr1 350 Forward 0 read043 DEL: chr1 335 Forward chr1 350 Forward 0 read044 DEL: chr1 335 Forward chr1 350 Forward 0 read045 +INS: chr1 367 Forward chr1 368 Forward 11 read046 +INS: chr1 367 Forward chr1 368 Forward 11 read047 +INS: chr1 367 Forward chr1 368 Forward 11 read048 +INS: chr1 367 Forward chr1 368 Forward 11 read049 +INS: chr1 367 Forward chr1 368 Forward 11 read050 +DEL: chr1 383 Forward chr1 395 Forward 0 read050 +BND: chr1 10 Reverse chr1 470 Reverse 0 read051 +BND: chr1 10 Reverse chr1 470 Reverse 0 read052 +BND: chr1 33 Forward chr1 471 Forward 0 read052 Start clustering... -Done with clustering. Found 11 junction clusters. +Done with clustering. Found 15 junction clusters. No refinement was selected. diff --git a/test/data/mini_example/output_res.txt b/test/data/mini_example/output_res.txt index 5d46becd..66372f18 100644 --- a/test/data/mini_example/output_res.txt +++ b/test/data/mini_example/output_res.txt @@ -1,6 +1,6 @@ ##fileformat=VCFv4.3 ##source=iGenVarCaller -##contig= +##contig= ##INFO= ##INFO= ##INFO= @@ -13,3 +13,5 @@ chr1 180 . N 1 PASS END=180;SVLEN=8;SVTYPE=INS GT ./. chr1 266 . N 4 PASS END=286;SVLEN=-20;SVTYPE=DEL GT ./. chr1 282 . N 1 PASS END=299;SVLEN=-17;SVTYPE=DEL GT ./. chr1 336 . N 4 PASS END=350;SVLEN=-14;SVTYPE=DEL GT ./. +chr1 368 . N 5 PASS END=368;SVLEN=11;SVTYPE=INS GT ./. +chr1 384 . 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zgXXW<>pq-sm3@`|a)4I6J>Gm%z(x3@72rbu=MU+P7@U7TI79`+3LJ0#6z4SX?s+oL zfNLHa5X=C1w{Bv&d^$Ak&m;dg{`-k1pN{Bz)4E-M;{Wa0|5rWhq7Uh7*WUgqc6|+{ z$_25Equ&|&H(ia1KGIKS-p#@mi%_4sn!_j$v)pF2L!Ann!{3;W6j2LzH>{}29q(Es-7eQx^$|M4<- vCfK)m`?7aG6KezF&UcT|NBbWfWE#TwWO2sKZSs%$ws`D1xRdntxBvcMVzghO diff --git a/test/data/mini_example/single_end_mini_example.sam b/test/data/mini_example/single_end_mini_example.sam index 0b344fe4..464a7aa6 100644 --- a/test/data/mini_example/single_end_mini_example.sam +++ b/test/data/mini_example/single_end_mini_example.sam @@ -1,5 +1,5 @@ @HD VN:1.6 SO:coordinate -@SQ SN:chr1 LN:368 +@SQ SN:chr1 LN:482 read001 0 chr1 1 60 50M * 0 0 CGCCCATGCAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCG * AS:i:50 NM:i:0 read002 0 chr1 2 60 50M * 0 0 GCCCATGCAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCGG * AS:i:50 NM:i:0 read003 0 chr1 3 60 50M * 0 0 CCCATGCAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCGGC * AS:i:50 NM:i:0 @@ -11,6 +11,8 @@ read008 0 chr1 8 60 50M * 0 0 GCAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCGGCGGCAT read009 0 chr1 9 60 49M1S * 0 0 CAACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCGGCGGCATA * AS:i:49 NM:i:0 read010 0 chr1 10 60 48M13D2M * 0 0 AACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCGGCGGCATAT * AS:i:46 NM:i:13 read011 0 chr1 11 60 47M13D3M * 0 0 ACTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCGGCGGCATATA * AS:i:46 NM:i:13 +read051 2048 chr1 11 60 27S23M * 0 0 CTGGGACGCTGTGACTGTACGGGGGGGACTAGCGATGCTAGCTAGCTAGC * AS:i:23 NM:i:0 +read052 2048 chr1 11 60 21S24M5S * 0 0 CGCTGTGACTGTACGGGGGGGACTAGCGATGCTAGCTAGCTAGCTCGGGC * AS:i:24 NM:i:0 read012 0 chr1 12 60 46M13D4M * 0 0 CTAGCGATGCTAGCTAGCTAGCTTACGACTGGCCATGCGGCGGCATATAC * AS:i:46 NM:i:13 read013 0 chr1 51 60 7M13D43M * 0 0 GCGGCATATACATAAGGGGCTCATCGATCGATTTCGGATCGGGGGGCCCC * AS:i:46 NM:i:13 read014 0 chr1 52 60 6M13D44M * 0 0 CGGCATATACATAAGGGGCTCATCGATCGATTTCGGATCGGGGGGCCCCC * AS:i:46 NM:i:13 @@ -70,3 +72,13 @@ read044 0 chr1 303 60 34M14D16M * 0 0 CCATGCGGATTATAAACGGGATATTAAGGGCTTTAGGGCTAG read044 256 chr1 303 60 20M14D30M * 0 0 CCATGCGGATTATAAACGGGATATTAAGGGCTTTAGGGCTAGCTAGGTCC * AS:i:46 NM:i:14 read045 0 chr1 305 60 32M14D18M * 0 0 ATGCGGATTATAAACGGGATATTAAGGGCTTTAGGGCTAGCTAGGTCCAA * AS:i:46 NM:i:14 read045 256 chr1 305 60 18M14D32M * 0 0 ATGCGGATTATAAACGGGATATTAAGGGCTTTAGGGCTAGCTAGGTCCAA * AS:i:46 NM:i:14 +read046 0 chr1 337 60 32M11I7M * 0 0 ATATTAAGGGCTTTAGGGCTAGCTAGGTCCAAGGTAACGTGTAAGCTTTC * AS:i:35 NM:i:16 +read047 0 chr1 338 60 31M11I8M * 0 0 TATTAAGGGCTTTAGGGCTAGCTAGGTCCAAGGTAACGTGTAAGCTTTCG * AS:i:35 NM:i:16 +read048 0 chr1 339 60 30M11I9M * 0 0 ATTAAGGGCTTTAGGGCTAGCTAGGTCCAAGGTAACGTGTAAGCTTTCGG * AS:i:35 NM:i:16 +read049 0 chr1 340 60 29M11I10M * 0 0 TTAAGGGCTTTAGGGCTAGCTAGGTCCAAGGTAACGTGTAAGCTTTCGGA * AS:i:35 NM:i:16 +read050 0 chr1 351 60 18M11I16M11D5M * 0 0 AGGGCTAGCTAGGTCCAAGGTAACGTGTAAGCTTTCGGAACGTACCGGTA * AS:i:40 NM:i:11 +read050 256 chr1 351 60 18M11I16M5S * 0 0 AGGGCTAGCTAGGTCCAAGGTAACGTGTAAGCTTTCGGAACGTACCGGTA * AS:i:35 NM:i:0 +read051 0 chr1 445 60 27M23S * 0 0 CTGGGACGCTGTGACTGTACGGGGGGGACTAGCGATGCTAGCTAGCTAGC * AS:i:27 NM:i:0 SA:Z:chr1,11,+,27S23M,60,0; +read052 0 chr1 451 60 21M29S * 0 0 CGCTGTGACTGTACGGGGGGGACTAGCGATGCTAGCTAGCTAGCTCGGGC * AS:i:24 NM:i:0 SA:Z:chr1,11,+,21S24M5S,60,0;chr1,472,+,45S5M,60,0; +read052 256 chr1 451 60 21M24I5M * 0 0 CGCTGTGACTGTACGGGGGGGACTAGCGATGCTAGCTAGCTAGCTCGGGC * AS:i:21 NM:i:0 +read052 2048 chr1 472 60 45S5M * 0 0 CGCTGTGACTGTACGGGGGGGACTAGCGATGCTAGCTAGCTAGCTCGGGC * AS:i:24 NM:i:0