Skip to content

Latest commit

 

History

History
67 lines (40 loc) · 2.18 KB

Software.md

File metadata and controls

67 lines (40 loc) · 2.18 KB
Raw

<Back

Software

The morbidostat control logic implemented on the Arduino programming language. GUI is designed with the MegunoLink interface tool.

Main principles of morbidostat logic are described in the Toprak et al paper [1].

Code could be found in the software/v4.2 folder

  • MorbidostatGUI4.2.mlx - MegunoLink GUI Interface
  • morbidostat2-7-18 - Arduino 1 code (main controls)
  • motorcontrol - Arduino 2 code (stepper motors control)

Graphical User Interface

GUI provides an interface to exchange information with Arduino board.

Interface has 3 major sections:

  1. Connection controls/Logs outputs controls
  2. Run controls
  3. Real-time plots/logs from the current run.

Software main

1. Connection controls/Logs outputs controls

In this part you can control general connection of computer to the morbidostat logic boards and set output log files.

  • Connection manager

Connection manager

  • Message logger

Message logger

2. Run controls

In this section you can control morbidostat process (turn on/of OD reading, media pumps, heater, air pump) and set parameters for the run (thresholds, dilution rates, temperature in the chamber, select tubes that are used in the experiment).

  • Control tab

Control tab

  • Settings tab

Settings tab

  • Agitation tab

Agitation tab

  • Calibration

Calibration tab

3. Real-time plots/logs from the current run

Sections shows OD plot and log of pumps activations.

  • OD plot

OD plot

  • Pump engagement log

!Pump log

References

[1]: Toprak E, Veres A, Yildiz S, Pedraza JM, Chait R, Paulsson J, Kishony R: Building a morbidostat: an automated continuous-culture device for studying bacterial drug resistance under dynamically sustained drug inhibition. Nat Protoc 2013, 8(3):555-567.
DOI: 10.1038/nprot.nprot.2013.021