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Data download guide

Browsing and downloading the data

The data used for the paper come in four sets:

  1. train: The train data, including predictions on the train data, as multi-channel images for easy viewing.
  2. train_single_channel_images: The train data, including predictions on the train data, as single-channel images. The filenames of these images identify the transmitted light modality or fluorescent label.
  3. test: The test data, including predictions on the test data, as multi-channel images for easy viewing.
  4. test_single_channel_images: The test data, including predictions on the test data, as single-channel images. The filenames of these images identify the transmitted light modality or fluorescent label.

These images may be viewed in a browser or downloaded in bulk using gsutil. The browser links are: train, train_single_channel_images, test, and test_single_channel_images.

To download in bulk, first install gsutil and execute commands like this:

gsutil -m cp -r gs://in-silico-labeling/paper_data/train .
gsutil -m cp -r gs://in-silico-labeling/paper_data/train_single_channel_images .
gsutil -m cp -r gs://in-silico-labeling/paper_data/test .
gsutil -m cp -r gs://in-silico-labeling/paper_data/test_single_channel_images .

Understanding the data

The file paths in the datasets have the following structure: <lab>/<condition>/<descriptive_filename>.png.

The naming for the labs and conditions differs from the paper. The key is:

  1. Rubin/scott_1_0 → Condition A.
  2. Finkbeiner/kevan_0_{7,8,9,10} → Condition B.
  3. Finkbeiner/alicia_2_0 → Condition C.
  4. Finkbeiner/yusha_0_1 → Condition D.
  5. GLS/2015_06_26 → Condition E.

The filenames themselves contain enough information to understand the contents of each image. For example, the following is the set of images from Well A4 in the kevan_0_8 condition: `

  1. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-DAPI_CONFOCAL,is_mask-false,kind,value-ORIGINAL.png
  2. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-DAPI_CONFOCAL,statistic,value-MEDIAN,kind,value-PREDICTED.png
  3. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-MAP2_CONFOCAL,is_mask-false,kind,value-ORIGINAL.png
  4. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-MAP2_CONFOCAL,statistic,value-MEDIAN,kind,value-PREDICTED.png
  5. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-NEURITE_CONFOCAL,is_mask-false,kind,value-ORIGINAL.png
  6. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-NEURITE_CONFOCAL,statistic,value-MEDIAN,kind,value-PREDICTED.png
  7. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-NFH_CONFOCAL,is_mask-false,kind,value-ORIGINAL.png
  8. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,depth_computation,value-MAXPROJECT,channel,value-NFH_CONFOCAL,statistic,value-MEDIAN,kind,value-PREDICTED.png
  9. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-0,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  10. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-10,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  11. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-11,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  12. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-12,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  13. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-1,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  14. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-2,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  15. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-3,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  16. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-4,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  17. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-5,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  18. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-6,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  19. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-7,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  20. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-8,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png
  21. lab-Finkbeiner,condition-kevan_0_8,acquisition_date,year-2015,month-9,day-28,well-A4,z_depth-9,channel,value-PHASE_CONTRAST,is_mask-false,kind,value-ORIGINAL.png

Thirteen of these images are the transmitted light z-stack: in this case the imaging modality was phase-contrast. The remaining eight are true and predicted fluorescence images for the DAPI_CONFOCAL, MAP2_CONFOCAL, NFH_CONFOCAL, and NEURITE_CONFOCAL channels. The NEURITE_CONFOCAL channel is a synthetic channel equal to the mean of the MAP2_CONFOCAL and NFH_CONFOCAL channels.

For this dataset you can ignore the is_mask field, which should always be false.

Citing the data

If you use this data, please cite our paper:

Christiansen E, Yang S, Ando D, Javaherian A, Skibinski G, Lipnick S, Mount E, O'Neil A, Shah K, Lee A, Goyal P, Fedus W, Poplin R, Esteva A, Berndl M, Rubin L, Nelson P, Finkbeiner S. In silico labeling: Predicting fluorescent labels in unlabeled images. Cell. 2018

BibTeX:

@article{christiansen2018isl,
  title={In silico labeling: Predicting fluorescent labels in unlabeled images},
  author={Christiansen, Eric M and Yang, Samuel J and Ando, D Michael and Javaherian, Ashkan and Skibinski, Gaia and Lipnick, Scott and Mount, Elliot and O’Neil, Alison and Shah, Kevan and Lee, Alicia K and Goyal, Piyush and Fedus, William and Poplin, Ryan and Esteva, Andre and Berndl, Marc and Rubin, Lee L and Nelson, Philip and Finkbeiner, Steven},
  journal={Cell},
  year={2018},
  publisher={Elsevier}
}

License

The data is licensed under the Creative Commons Attribution 4.0 International license.

The following groups contributed to the creation of the dataset:

  1. The Rubin Lab at Harvard: Lab work and imaging for Condition A.
  2. The Finkbeiner Lab at Gladstone: Lab work and imaging for Conditions B, C, and D.
  3. Google Accelerated Science: Lab work and imaging for Condition E and preprocessing for Conditions A, B, C, D, and E.