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I must admit I am out of my depth here. This boils down to how Python handles unicode. AFAIK this is different in Python2 and Python3. Anyway, I would try to simplify it to a minimal example (few lines of Python code) and then ask at stackoverflow to get an opinion from Python experts.
I use python 3.4.
Generating code with the error info:
Traceback (most recent call last):
File "ribosome.py", line 371, in
line = dnastack[-1][0].readline()
UnicodeDecodeError: 'gbk' codec can't decode byte 0xae in position 285: illegal
multibyte sequence
TAS57x7_Param_c_tpl.py.dna.txt
My *.dna file has been attached.
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