diff --git a/instrumentation/opentelemetry-instrumentation-pymysql/tests/test_pymysql_integration.py b/instrumentation/opentelemetry-instrumentation-pymysql/tests/test_pymysql_integration.py
index 6f8af5d8df..18b1f0329b 100644
--- a/instrumentation/opentelemetry-instrumentation-pymysql/tests/test_pymysql_integration.py
+++ b/instrumentation/opentelemetry-instrumentation-pymysql/tests/test_pymysql_integration.py
@@ -111,6 +111,42 @@ def test_instrument_connection(self, mock_connect):
         spans_list = self.memory_exporter.get_finished_spans()
         self.assertEqual(len(spans_list), 1)
 
+    @mock.patch("opentelemetry.instrumentation.dbapi.instrument_connection")
+    @mock.patch("pymysql.connect")
+    # pylint: disable=unused-argument
+    def test_instrument_connection_enable_commenter(
+        self,
+        mock_connect,
+        mock_instrument_connection,
+    ):
+        cnx = pymysql.connect(database="test")
+        cnx = PyMySQLInstrumentor().instrument_connection(
+            cnx,
+            enable_commenter=True,
+            commenter_options={"foo": True},
+        )
+        cursor = cnx.cursor()
+        cursor.execute("SELECT * FROM test")
+        kwargs = mock_instrument_connection.call_args[1]
+        self.assertEqual(kwargs["enable_commenter"], True)
+        self.assertEqual(kwargs["commenter_options"], {"foo": True})
+
+    @mock.patch("opentelemetry.instrumentation.dbapi.wrap_connect")
+    @mock.patch("pymysql.connect")
+    # pylint: disable=unused-argument
+    def test__instrument_enable_commenter(
+        self,
+        mock_connect,
+        mock_wrap_connect,
+    ):
+        PyMySQLInstrumentor()._instrument(
+            enable_commenter=True,
+            commenter_options={"foo": True},
+        )
+        kwargs = mock_wrap_connect.call_args[1]
+        self.assertEqual(kwargs["enable_commenter"], True)
+        self.assertEqual(kwargs["commenter_options"], {"foo": True})
+
     @mock.patch("pymysql.connect")
     # pylint: disable=unused-argument
     def test_uninstrument_connection(self, mock_connect):