Main tool : bcftools
bcftools mpileup -A -d 200 -B -Q 0 -f {reference_genome} {bam} | bcftools call -mv -Ov -o bcftools_variants/{sample}.vcf
Better documentation can be found at https://www.htslib.org/doc/bcftools.html
Main tool : bcftools
bcftools mpileup -A -d 200 -B -Q 0 -f {reference_genome} {bam} | bcftools call -mv -Ov -o bcftools_variants/{sample}.vcf
Better documentation can be found at https://www.htslib.org/doc/bcftools.html