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README.md

Assessing genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs (BUSCO) container

Main tool : BUSCO

Additional tools:

  • BBTools 39.06
  • HMMER 3.3
  • Prodigal 2.6.3
  • SEPP 4.5.1
  • Python 3.8.10
  • BioPython 1.83
  • Perl 5.30.0
  • OpenJDK 1.8.0_392

Full documentation: https://busco.ezlab.org/busco_userguide.html

This is a BUSCO docker image which has basic functions for prokaryotes only. This image contains bacteria_odb10 lineage dataset for offline use.

Example Usage

# offline usage with bacteria lineage
busco --offline -i assembly.fasta -l /busco_downloads/lineages/bacteria_odb10 -o output -m genome
# auto lineage selection
busco -i assembly.fasta -o output -m genome --auto-lineage-prok