Main tool : skani
Full documentation: https://github.com/bluenote-1577/skani/wiki
skani is a program for calculating average nucleotide identity (ANI) from DNA sequences (contigs/MAGs/genomes) for ANI > ~80%.
Quick ANI calculation:
skani dist genome1.fa genome2.fa
Memory-efficient database search:
skani sketch genomes/* -o database
skani search -d database query1.fa query2.fa ...
All-to-all comparison:
skani triangle genomes/*