From 3e8c4474400b621dbb5f5ec7f193091f7e981eb9 Mon Sep 17 00:00:00 2001 From: Curtis Kapsak Date: Thu, 24 Oct 2024 16:18:31 -0400 Subject: [PATCH] [TheiaProk] Adds stxtyper to merlin_magic and TheiaProk wfs (#525) * added WDL task for testing StxTyper. ran successfully with miniwdl * fix typo in stxtyper task output * added stxtyper to merlin_magic subwf and theiaprok_illumina_pe. NOT TESTED locally. it may not work * added log file output to stxtyper task, merlin_magic subwf, and theiaprok_illumina_pe wf. Also added code to catch when stxtyper output TSV is one line meaning no hits were found * big change to stxtyper outputs. removed many and replaced with 2 outputs: String stxtyper_hits and Int stxtyper_num_hits. updated merlin_magic and theiaprok_illumina_PE workflow and tested successfully w miniwdl * update stxtyper_num_hits output when 0 hits are found * move stxtyper call block in merlin_magic wf so that all Escherichia and Shigella (including sonnei) are run through stxtyper * update to more recent stxtyper docker image that includes 2 new output columns; downsize disk_size to 50 gb default instead of 100 (it doesn't need much as it runs on 1 assembly * reduced down to 1 cpu and 2 GB memory as I think it runs singlethreaded; preemptible on since this task doesn't run for much more than 2 min; created outputs in case no hits are found; started re-writing parsing code for when hits are detected & finished the bit for complete operons. tested successfully with miniwdl * big update to stxtyper output parsing. runs successfully, but still needs work to iron out bugs. want to save progress and push a commit * updated merlin_magic with stxtyper optional inputs so they are exposed to user; major changes to stxtyper parsing again; added a new optional input & 1 output, removed 1 output; still needs work * updated one stxtyper output string name; updated merlin_magic subwf with new stxtyper outputs; updated theiaprok_illumina_pe with new stxtyper outputs; still need to rework part of parsing for stxtyper but theiaprok ran successfully w miniwdl * more updates to stxtyper output parsing. tested on many samples w miniwdl successfully * shorten optional input variable name for stxtyper * revert to 3 maxRetries stxtyper task * stxtyper increase memory request to 4 GB * add stxtyper outputs to theiaprok_fasta wf. tested successfully w miniwdl * major update to stxtyper WDL task. updated docker image to v1.0.24; added boolean for enabling built-in stxtyper debugging; removed many outputs and added stxtyper_all_hits output; also renamed a few outputs for clarity and consistency; commented out unused portions of code which will be deleted later * updated inputs/outputs for stxtyper in merlin_magic subwf, updated theiaprok ilmn pe wf outputs; adjusted order outputs in stxtyper task for clarity * merlin_magic: moved stxtyper call block outside of Escherichia/shigella call block. added optional input call_stxtyper so user can run tool regardless of merlin_tag and GAMBIT_predicted_taxon. tested successfully w miniwdl, need to test in Terra * cleanup unused code from stxtyper task; adjust code block for when no hits are found and output files are created * update theiaprok fasta, ilmn SE, ONT with stxtyper outputs. need to test in Terra * update CI * update CI * added TheiaProk workflow inputs and stxtyper block describing the tool to the theiaprok documentation. also fixed a minor comment typo in stxtyper task file * added stxtyper outputs to theiaprok docs page * updated theiaprok diagram to include stxtyper under Escherichia spp. and Shigella spp specific tasks * added punctuation * update ci * remove stxtyper_log File output from workflows as it's only slighly useful for debugging and not useful to end user. also removed mention in the docs * update ci * update to static link in docs * updated the correct/most up-to-date theiaprok diagram * update theiaprok diagram thank you @sage-wright for help on updating this diagram! --- docs/assets/figures/TheiaProk.png | Bin 97994 -> 211877 bytes .../genomic_characterization/theiaprok.md | 75 ++++++++--- .../escherichia_shigella/task_stxtyper.wdl | 122 ++++++++++++++++++ .../test_wf_theiaprok_illumina_pe.yml | 4 +- .../test_wf_theiaprok_illumina_se.yml | 4 +- workflows/theiaprok/wf_theiaprok_fasta.wdl | 10 ++ .../theiaprok/wf_theiaprok_illumina_pe.wdl | 10 ++ .../theiaprok/wf_theiaprok_illumina_se.wdl | 10 ++ workflows/theiaprok/wf_theiaprok_ont.wdl | 10 ++ workflows/utilities/wf_merlin_magic.wdl | 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zB)05dy>g5jWnR_JDn40Xj%Kx|<4_;ji9T}uKZFx4g5!$;g(R%$44fL~|Iu2vuV0EFfRp|#3V0kEfqo3QbMRMpVE{Qng6nGk z$G@03!_W5^l&08xf`!@YztLVcis^`2+URgx^VQrfYAzJU;~3xmWOTuU0Qc#{ zWMwo9wcymLQ;)KW(L;BpQ|&k9KYjl5UmP|D?lrs8lmqT+;+s&bZ~rb~Onpx$c7`rc;JY-2R zp`oFHhZ>?o-Oh!&Yr2NGgAW=d1tk?Z1vNP(H5&yDO%)AI1r?-%f~JB(7$!LQKMME< wx_Ns<{O<)An4b)Q0@DAq5bEvkem?Y^f6)J4<}B|bFDOG}Y-n+^MjxB-KcIg&!T | -??? toggle "_Neisseria_ spp" - ##### _Neisseria_ spp {#neisseria} +??? toggle "_Neisseria_ spp." + ##### _Neisseria_ spp. {#neisseria} ??? task "`ngmaster`: _Neisseria gonorrhoeae_ sequence typing" NG-MAST is currently the most widely used method for epidemiological surveillance of *Neisseria gonorrhoea.* This tool is targeted at clinical and research microbiology laboratories that have performed WGS of *N. gonorrhoeae* isolates and wish to understand the molecular context of their data in comparison to previously published epidemiological studies. As WGS becomes more routinely performed, *NGMASTER* @@ -1386,14 +1416,14 @@ The TheiaProk workflows automatically activate taxa-specific sub-workflows after | Software Documentation | [pasty](https://github.com/rpetit3/pasty) | | Original Publication(s) | [Application of Whole-Genome Sequencing Data for O-Specific Antigen Analysis and In Silico Serotyping of Pseudomonas aeruginosa Isolates.](https://journals.asm.org/doi/10.1128/JCM.00349-16) | -??? toggle "_Salmonella_ spp" - ##### _Salmonella_ spp {#salmonella} +??? toggle "_Salmonella_ spp." + ##### _Salmonella_ spp. {#salmonella} Both SISTR and SeqSero2 are used for serotyping all *Salmonella* spp. Occasionally, the predicted serotypes may differ between SISTR and SeqSero2. When this occurs, differences are typically small and analogous, and are likely as a result of differing source databases. More information about Salmonella serovar nomenclature can be found [here](https://www.happykhan.com/posts/binfie-guide-serovar/). For *Salmonella* Typhi, genotyphi is additionally run for further typing. ??? task "`SISTR`: Salmonella serovar prediction" - [SISTR](https://github.com/phac-nml/sistr_cmd) performs *Salmonella spp* serotype prediction using antigen gene and cgMLST gene alleles. In TheiaProk. SISTR is run on genome assemblies, and uses the default database setting (smaller "centroid" alleles or representative alleles instead of the full set of cgMLST alleles). It also runs a QC mode to determine the level of confidence in the serovar prediction (see [here](https://github.com/phac-nml/sistr_cmd#qc-by-sistr_cmd---qc)). + [SISTR](https://github.com/phac-nml/sistr_cmd) performs *Salmonella spp.* serotype prediction using antigen gene and cgMLST gene alleles. In TheiaProk. SISTR is run on genome assemblies, and uses the default database setting (smaller "centroid" alleles or representative alleles instead of the full set of cgMLST alleles). It also runs a QC mode to determine the level of confidence in the serovar prediction (see [here](https://github.com/phac-nml/sistr_cmd#qc-by-sistr_cmd---qc)). !!! techdetails "SISTR Technical Details" @@ -1534,11 +1564,11 @@ The TheiaProk workflows automatically activate taxa-specific sub-workflows after | Software Source Code | [emm-typing-tool](https://github.com/ukhsa-collaboration/emm-typing-tool) | | Software Documentation | [emm-typing-tool](https://github.com/ukhsa-collaboration/emm-typing-tool) | -??? toggle "_Vibrio_ spp" - ##### _Vibrio_ spp {#vibrio} +??? toggle "_Vibrio_ spp." + ##### _Vibrio_ spp. {#vibrio} ??? task "`SRST2`: Vibrio characterization ==_for Illumina only_==" - The `SRST2 Vibrio characterization` task detects sequences for *Vibrio* spp characterization using Illumina sequence reads and a database of target sequence that are traditionally used in PCR methods. The sequences included in the database are as follows: + The `SRST2 Vibrio characterization` task detects sequences for *Vibrio* spp. characterization using Illumina sequence reads and a database of target sequence that are traditionally used in PCR methods. The sequences included in the database are as follows: | Sequence name | Sequence role | Purpose in database | | --- | --- | --- | @@ -1561,7 +1591,7 @@ The TheiaProk workflows automatically activate taxa-specific sub-workflows after ??? task "`Abricate`: Vibrio characterization" - The `Abricate` Vibrio characterization task detects sequences for *Vibrio* spp characterization using genome assemblies and the abricate "vibrio" database. The sequences included in the database are as follows: + The `Abricate` Vibrio characterization task detects sequences for *Vibrio* spp. characterization using genome assemblies and the abricate "vibrio" database. The sequences included in the database are as follows: | Sequence name | Sequence role | Purpose in database | | --- | --- | --- | @@ -1865,7 +1895,7 @@ The TheiaProk workflows automatically activate taxa-specific sub-workflows after | resfinder_results | File | Predicted resistance genes grouped by antibiotic class | FASTA, ONT, PE, SE | | resfinder_seqs | File | FASTA of resistance gene sequences from user’s input sequence | FASTA, ONT, PE, SE | | seq_platform | String | Sequencing platform input by the user | FASTA, ONT, PE, SE | -| seqsero2_predicted_antigenic_profile | String | Antigenic profile predicted for Salmonella spp by SeqSero2 | ONT, PE, SE | +| seqsero2_predicted_antigenic_profile | String | Antigenic profile predicted for Salmonella spp. by SeqSero2 | ONT, PE, SE | | seqsero2_predicted_contamination | String | Indicates whether contamination between Salmonella with different serotypes was predicted by SeqSero2 | ONT, PE, SE | | seqsero2_predicted_serotype | String | Serotype predicted by SeqSero2 | ONT, PE, SE | | seqsero2_report | File | TSV report produced by SeqSero2 | ONT, PE, SE | @@ -1940,6 +1970,15 @@ The TheiaProk workflows automatically activate taxa-specific sub-workflows after | staphopiasccmec_results_tsv | File | sccmec types and mecA presence | FASTA, ONT, PE, SE | | staphopiasccmec_types_and_mecA_presence | String | staphopia-sccmec Hamming distance file | FASTA, ONT, PE, SE | | staphopiasccmec_version | String | staphopia-sccmec presence and absence TSV file | FASTA, ONT, PE, SE | +| stxtyper_all_hits | String | Comma-separated list of matches of all types. Includes complete, partial, frameshift, internal stop, and novel hits. List is de-duplicated so multiple identical hits are only listed once. For example if 5 partial stx2 hits are detected in the genome, only 1 "stx2" will be listed in this field. To view the potential subtype for each partial hit, the user will need to view the stxtyper_report TSV file. | FASTA, ONT, PE, SE | +| stxtyper_complete_operons | String | Comma-separated list of all COMPLETE operons detected by StxTyper. Show multiple hits if present in results. | FASTA, ONT, PE, SE | +| stxtyper_docker | String | Name of docker image used by the stxtyper task. | FASTA, ONT, PE, SE | +| stxtyper_novel_hits | String | Comma-separated list of matches that have the OPERON output of "COMPLETE_NOVEL". Possible outputs "stx1", "stx2", or "stx1,stx2" | FASTA, ONT, PE, SE | +| stxtyper_num_hits | Int | Number of "hits" or rows present in the `stxtyper_report` TSV file | FASTA, ONT, PE, SE | +| stxtyper_partial_hits | String | Possible outputs "stx1", "stx2", or "stx1,stx2". Tells the user that there was a partial hit to either the A or B subunit, but does not describe which subunit, only the possible types from the PARTIAL matches. | FASTA, ONT, PE, SE | +| stxtyper_report | File | Raw results TSV file produced by StxTyper | FASTA, ONT, PE, SE | +| stxtyper_stx_frameshifts_or_internal_stop_hits | String | Comma-separated list of matches that have the OPERON output of "FRAMESHIFT" or "INTERNAL_STOP". Possible outputs "stx1", "stx2", or "stx1,stx2" | FASTA, ONT, PE, SE | +| stxtyper_version | String | Version of StxTyper used | FASTA, ONT, PE, SE | | taxon_table_status | String | Status of the taxon table upload | FASTA, ONT, PE, SE | | tbp_parser_average_genome_depth | Float | Optional output. Average genome depth across the reference genome | ONT, PE, SE | | tbp_parser_coverage_report | File | Optional output. TSV file with breadth of coverage of each gene associated with antimicrobial resistance in mycobacterium tuberculosis. | ONT, PE, SE | diff --git a/tasks/species_typing/escherichia_shigella/task_stxtyper.wdl b/tasks/species_typing/escherichia_shigella/task_stxtyper.wdl new file mode 100644 index 000000000..f066ad109 --- /dev/null +++ b/tasks/species_typing/escherichia_shigella/task_stxtyper.wdl @@ -0,0 +1,122 @@ +version 1.0 + +task stxtyper { + input { + File assembly + String samplename + Boolean enable_debugging = false # Additional messages are printed and files in $TMPDIR are not removed after running + String docker = "us-docker.pkg.dev/general-theiagen/staphb/stxtyper:1.0.24" + Int disk_size = 50 + Int cpu = 1 + Int memory = 4 + } + command <<< + # fail task if any commands below fail since there's lots of bash conditionals below (AGH!) + set -eo pipefail + + # capture version info + stxtyper --version | tee VERSION.txt + + # NOTE: by default stxyper uses $TMPDIR or /tmp, so if we run into issues we may need to adjust in the future. Could potentially use PWD as the TMPDIR. + echo "DEBUG: TMPDIR is set to: $TMPDIR" + + echo "DEBUG: running StxTyper now..." + # run StxTyper on assembly; may need to add/remove options in the future if they change + # NOTE: stxtyper can accept gzipped assemblies, so no need to unzip + stxtyper \ + --nucleotide ~{assembly} \ + --name ~{samplename} \ + --output ~{samplename}_stxtyper.tsv \ + ~{true='--debug' false='' enable_debugging} \ + --log ~{samplename}_stxtyper.log + + # parse output TSV + echo "DEBUG: Parsing StxTyper output TSV..." + + # check for output file with only 1 line (meaning no hits found); exit cleanly if so + if [ "$(wc -l < ~{samplename}_stxtyper.tsv)" -eq 1 ]; then + echo "No hits found by StxTyper" > stxtyper_hits.txt + echo "0" > stxtyper_num_hits.txt + echo "DEBUG: No hits found in StxTyper output TSV. Exiting task with exit code 0 now." + + # create empty output files + touch stxtyper_all_hits.txt stxtyper_complete_operons.txt stxtyper_partial_hits.txt stxtyper_stx_frameshifts_or_internal_stop_hits.txt stx_novel_hits.txt + # put "none" into all of them so task does not fail + echo "None" | tee stxtyper_all_hits.txt stxtyper_complete_operons.txt stxtyper_partial_hits.txt stxtyper_stx_frameshifts_or_internal_stop_hits.txt stx_novel_hits.txt + exit 0 + fi + + # check for output file with more than 1 line (meaning hits found); count lines & parse output TSV if so + if [ "$(wc -l < ~{samplename}_stxtyper.tsv)" -gt 1 ]; then + echo "Hits found by StxTyper. Counting lines & parsing output TSV now..." + # count number of lines in output TSV (excluding header) + wc -l < ~{samplename}_stxtyper.tsv | awk '{print $1-1}' > stxtyper_num_hits.txt + # remove header line + sed '1d' ~{samplename}_stxtyper.tsv > ~{samplename}_stxtyper_noheader.tsv + + ##### parse output TSV ##### + ### complete operons + echo "DEBUG: Parsing complete operons..." + awk -F'\t' -v OFS=, '$4 == "COMPLETE" {print $3}' ~{samplename}_stxtyper.tsv | paste -sd, - | tee stxtyper_complete_operons.txt + # if grep for COMPLETE fails, write "None" to file for output string + if [[ "$(grep --silent 'COMPLETE' ~{samplename}_stxtyper.tsv; echo $?)" -gt 0 ]]; then + echo "None" > stxtyper_complete_operons.txt + fi + + ### complete_novel operons + echo "DEBUG: Parsing complete novel hits..." + awk -F'\t' -v OFS=, '$4 == "COMPLETE_NOVEL" {print $3}' ~{samplename}_stxtyper.tsv | paste -sd, - | tee stx_novel_hits.txt + # if grep for COMPLETE_NOVEL fails, write "None" to file for output string + if [ "$(grep --silent 'COMPLETE_NOVEL' ~{samplename}_stxtyper.tsv; echo $?)" -gt 0 ]; then + echo "None" > stx_novel_hits.txt + fi + + ### partial hits (to any gene in stx operon) + echo "DEBUG: Parsing stxtyper partial hits..." + # explanation: if "operon" column contains "PARTIAL" (either PARTIAL or PARTIAL_CONTIG_END possible); print either "stx1" or "stx2" or "stx1,stx2" + awk -F'\t' -v OFS=, '$4 ~ "PARTIAL.*" {print $3}' ~{samplename}_stxtyper.tsv | sort | uniq | paste -sd, - | tee stxtyper_partial_hits.txt + # if no stx partial hits found, write "None" to file for output string + if [ "$(grep --silent 'stx' stxtyper_partial_hits.txt; echo $?)" -gt 0 ]; then + echo "None" > stxtyper_partial_hits.txt + fi + + ### frameshifts or internal stop codons in stx genes + echo "DEBUG: Parsing stx frameshifts or internal stop codons..." + # explanation: if operon column contains "FRAME_SHIFT" or "INTERNAL_STOP", print the "operon" in a sorted/unique list + awk -F'\t' -v OFS=, '$4 == "FRAMESHIFT" || $4 == "INTERNAL_STOP" {print $3}' ~{samplename}_stxtyper.tsv | sort | uniq | paste -sd, - | tee stxtyper_stx_frameshifts_or_internal_stop_hits.txt + # if no frameshifts or internal stop codons found, write "None" to file for output string + if [ "$(grep --silent -E 'FRAMESHIFT|INTERNAL_STOP' ~{samplename}_stxtyper.tsv; echo $?)" -gt 0 ]; then + echo "None" > stxtyper_stx_frameshifts_or_internal_stop_hits.txt + fi + + echo "DEBUG: generating stx_type_all string output now..." + # sort and uniq so there are no duplicates; then paste into a single comma-separated line with commas + # sed is to remove any instances of "None" from the output + cat stxtyper_complete_operons.txt stxtyper_partial_hits.txt stxtyper_stx_frameshifts_or_internal_stop_hits.txt stx_novel_hits.txt | sed '/None/d' | sort | uniq | paste -sd, - > stxtyper_all_hits.txt + + fi + echo "DEBUG: Finished parsing StxTyper output TSV." + >>> + output { + File stxtyper_report = "~{samplename}_stxtyper.tsv" + File stxtyper_log = "~{samplename}_stxtyper.log" + String stxtyper_docker = docker + String stxtyper_version = read_string("VERSION.txt") + # outputs parsed from stxtyper output TSV + Int stxtyper_num_hits = read_int("stxtyper_num_hits.txt") + String stxtyper_all_hits = read_string("stxtyper_all_hits.txt") + String stxtyper_complete_operon_hits = read_string("stxtyper_complete_operons.txt") + String stxtyper_partial_hits = read_string("stxtyper_partial_hits.txt") + String stxtyper_frameshifts_or_internal_stop_hits = read_string("stxtyper_stx_frameshifts_or_internal_stop_hits.txt") + String stxtyper_novel_hits = read_string("stx_novel_hits.txt") + } + runtime { + docker: "~{docker}" + memory: "~{memory} GB" + cpu: cpu + disks: "local-disk " + disk_size + " SSD" + disk: disk_size + " GB" + preemptible: 1 # does not take long (usually <3 min) to run stxtyper on 1 genome, preemptible is fine + maxRetries: 3 + } +} diff --git a/tests/workflows/theiaprok/test_wf_theiaprok_illumina_pe.yml b/tests/workflows/theiaprok/test_wf_theiaprok_illumina_pe.yml index 91ae801b7..71f5bd4a2 100644 --- a/tests/workflows/theiaprok/test_wf_theiaprok_illumina_pe.yml +++ b/tests/workflows/theiaprok/test_wf_theiaprok_illumina_pe.yml @@ -631,9 +631,9 @@ - path: miniwdl_run/wdl/tasks/utilities/data_export/task_broad_terra_tools.wdl md5sum: 4d69a6539b68503af9f3f1c2787ff920 - path: miniwdl_run/wdl/workflows/theiaprok/wf_theiaprok_illumina_pe.wdl - md5sum: 6d9dd969e2144ca23f2a0e101e6b6966 + md5sum: 3cb5c86b15e931b0c0b98ed784386438 - path: miniwdl_run/wdl/workflows/utilities/wf_merlin_magic.wdl - md5sum: 670f990128063eb3c7b3fa49302f08b7 + md5sum: ea5cff6eff8c2c42046cf2eae6f16b6f - path: miniwdl_run/wdl/workflows/utilities/wf_read_QC_trim_pe.wdl contains: ["version", "QC", "output"] - path: miniwdl_run/workflow.log diff --git a/tests/workflows/theiaprok/test_wf_theiaprok_illumina_se.yml b/tests/workflows/theiaprok/test_wf_theiaprok_illumina_se.yml index 82f9a9a74..88584182b 100644 --- a/tests/workflows/theiaprok/test_wf_theiaprok_illumina_se.yml +++ b/tests/workflows/theiaprok/test_wf_theiaprok_illumina_se.yml @@ -594,9 +594,9 @@ - path: miniwdl_run/wdl/tasks/utilities/data_export/task_broad_terra_tools.wdl md5sum: 4d69a6539b68503af9f3f1c2787ff920 - path: miniwdl_run/wdl/workflows/theiaprok/wf_theiaprok_illumina_se.wdl - md5sum: 5aa25e4fad466f92c96a7c138aca0d20 + md5sum: fdb66b59ac886501a4ae90a25cefd633 - path: miniwdl_run/wdl/workflows/utilities/wf_merlin_magic.wdl - md5sum: 670f990128063eb3c7b3fa49302f08b7 + md5sum: ea5cff6eff8c2c42046cf2eae6f16b6f - path: miniwdl_run/wdl/workflows/utilities/wf_read_QC_trim_se.wdl md5sum: d11bfe33fdd96eab28892be5a01c1c7d - path: miniwdl_run/workflow.log diff --git a/workflows/theiaprok/wf_theiaprok_fasta.wdl b/workflows/theiaprok/wf_theiaprok_fasta.wdl index 3735bda10..4ce8e5cb2 100644 --- a/workflows/theiaprok/wf_theiaprok_fasta.wdl +++ b/workflows/theiaprok/wf_theiaprok_fasta.wdl @@ -576,6 +576,16 @@ workflow theiaprok_fasta { File? virulencefinder_report_tsv = merlin_magic.virulencefinder_report_tsv String? virulencefinder_docker = merlin_magic.virulencefinder_docker String? virulencefinder_hits = merlin_magic.virulencefinder_hits + # stxtyper + File? stxtyper_report = merlin_magic.stxtyper_report + String? stxtyper_docker = merlin_magic.stxtyper_docker + String? stxtyper_version = merlin_magic.stxtyper_version + Int? stxtyper_num_hits = merlin_magic.stxtyper_num_hits + String? stxtyper_all_hits = merlin_magic.stxtyper_all_hits + String? stxtyper_complete_operons = merlin_magic.stxtyper_complete_operon_hits + String? stxtyper_partial_hits = merlin_magic.stxtyper_partial_hits + String? stxtyper_stx_frameshifts_or_internal_stop_hits = merlin_magic.stxtyper_stx_frameshifts_or_internal_stop_hits + String? stxtyper_novel_hits = merlin_magic.stxtyper_novel_hits # Listeria Typing File? lissero_results = merlin_magic.lissero_results String? lissero_version = merlin_magic.lissero_version diff --git a/workflows/theiaprok/wf_theiaprok_illumina_pe.wdl b/workflows/theiaprok/wf_theiaprok_illumina_pe.wdl index fd2e7dea1..d71c5e324 100644 --- a/workflows/theiaprok/wf_theiaprok_illumina_pe.wdl +++ b/workflows/theiaprok/wf_theiaprok_illumina_pe.wdl @@ -819,6 +819,16 @@ workflow theiaprok_illumina_pe { File? virulencefinder_report_tsv = merlin_magic.virulencefinder_report_tsv String? virulencefinder_docker = merlin_magic.virulencefinder_docker String? virulencefinder_hits = merlin_magic.virulencefinder_hits + # stxtyper + File? stxtyper_report = merlin_magic.stxtyper_report + String? stxtyper_docker = merlin_magic.stxtyper_docker + String? stxtyper_version = merlin_magic.stxtyper_version + Int? stxtyper_num_hits = merlin_magic.stxtyper_num_hits + String? stxtyper_all_hits = merlin_magic.stxtyper_all_hits + String? stxtyper_complete_operons = merlin_magic.stxtyper_complete_operon_hits + String? stxtyper_partial_hits = merlin_magic.stxtyper_partial_hits + String? stxtyper_stx_frameshifts_or_internal_stop_hits = merlin_magic.stxtyper_stx_frameshifts_or_internal_stop_hits + String? stxtyper_novel_hits = merlin_magic.stxtyper_novel_hits # Shigella sonnei Typing File? sonneityping_mykrobe_report_csv = merlin_magic.sonneityping_mykrobe_report_csv File? sonneityping_mykrobe_report_json = merlin_magic.sonneityping_mykrobe_report_json diff --git a/workflows/theiaprok/wf_theiaprok_illumina_se.wdl b/workflows/theiaprok/wf_theiaprok_illumina_se.wdl index 0e00e3ac8..1c3eee081 100644 --- a/workflows/theiaprok/wf_theiaprok_illumina_se.wdl +++ b/workflows/theiaprok/wf_theiaprok_illumina_se.wdl @@ -758,6 +758,16 @@ workflow theiaprok_illumina_se { File? virulencefinder_report_tsv = merlin_magic.virulencefinder_report_tsv String? virulencefinder_docker = merlin_magic.virulencefinder_docker String? virulencefinder_hits = merlin_magic.virulencefinder_hits + # stxtyper + File? stxtyper_report = merlin_magic.stxtyper_report + String? stxtyper_docker = merlin_magic.stxtyper_docker + String? stxtyper_version = merlin_magic.stxtyper_version + Int? stxtyper_num_hits = merlin_magic.stxtyper_num_hits + String? stxtyper_all_hits = merlin_magic.stxtyper_all_hits + String? stxtyper_complete_operons = merlin_magic.stxtyper_complete_operon_hits + String? stxtyper_partial_hits = merlin_magic.stxtyper_partial_hits + String? stxtyper_stx_frameshifts_or_internal_stop_hits = merlin_magic.stxtyper_stx_frameshifts_or_internal_stop_hits + String? stxtyper_novel_hits = merlin_magic.stxtyper_novel_hits # Shigella sonnei Typing File? sonneityping_mykrobe_report_csv = merlin_magic.sonneityping_mykrobe_report_csv File? sonneityping_mykrobe_report_json = merlin_magic.sonneityping_mykrobe_report_json diff --git a/workflows/theiaprok/wf_theiaprok_ont.wdl b/workflows/theiaprok/wf_theiaprok_ont.wdl index fecfb744e..a7eb9143e 100644 --- a/workflows/theiaprok/wf_theiaprok_ont.wdl +++ b/workflows/theiaprok/wf_theiaprok_ont.wdl @@ -730,6 +730,16 @@ workflow theiaprok_ont { File? virulencefinder_report_tsv = merlin_magic.virulencefinder_report_tsv String? virulencefinder_docker = merlin_magic.virulencefinder_docker String? virulencefinder_hits = merlin_magic.virulencefinder_hits + # stxtyper + File? stxtyper_report = merlin_magic.stxtyper_report + String? stxtyper_docker = merlin_magic.stxtyper_docker + String? stxtyper_version = merlin_magic.stxtyper_version + Int? stxtyper_num_hits = merlin_magic.stxtyper_num_hits + String? stxtyper_all_hits = merlin_magic.stxtyper_all_hits + String? stxtyper_complete_operons = merlin_magic.stxtyper_complete_operon_hits + String? stxtyper_partial_hits = merlin_magic.stxtyper_partial_hits + String? stxtyper_stx_frameshifts_or_internal_stop_hits = merlin_magic.stxtyper_stx_frameshifts_or_internal_stop_hits + String? stxtyper_novel_hits = merlin_magic.stxtyper_novel_hits # Shigella sonnei Typing File? sonneityping_mykrobe_report_csv = merlin_magic.sonneityping_mykrobe_report_csv File? sonneityping_mykrobe_report_json = merlin_magic.sonneityping_mykrobe_report_json diff --git a/workflows/utilities/wf_merlin_magic.wdl b/workflows/utilities/wf_merlin_magic.wdl index 1d6914e79..f10060851 100644 --- a/workflows/utilities/wf_merlin_magic.wdl +++ b/workflows/utilities/wf_merlin_magic.wdl @@ -8,6 +8,7 @@ import "../../tasks/species_typing/escherichia_shigella/task_serotypefinder.wdl" import "../../tasks/species_typing/escherichia_shigella/task_shigatyper.wdl" as shigatyper_task import "../../tasks/species_typing/escherichia_shigella/task_shigeifinder.wdl" as shigeifinder_task import "../../tasks/species_typing/escherichia_shigella/task_sonneityping.wdl" as sonneityping_task +import "../../tasks/species_typing/escherichia_shigella/task_stxtyper.wdl" as stxtyper_task import "../../tasks/species_typing/escherichia_shigella/task_virulencefinder.wdl" as virulencefinder_task import "../../tasks/species_typing/haemophilus/task_hicap.wdl" as hicap_task import "../../tasks/species_typing/klebsiella/task_kleborate.wdl" as kleborate_task @@ -218,6 +219,13 @@ workflow merlin_magic { Float? virulencefinder_coverage_threshold Float? virulencefinder_identity_threshold String? virulencefinder_database + # stxtyper options + Boolean call_stxtyper = false # set to true to run stxtyper on any bacterial sample + Boolean? stxtyper_enable_debug + String? stxtyper_docker_image + Int? stxtyper_disk_size + Int? stxtyper_cpu + Int? stxtyper_memory } # theiaprok if (merlin_tag == "Acinetobacter baumannii") { @@ -241,6 +249,19 @@ workflow merlin_magic { docker = abricate_abaum_docker_image } } + # stxtyper is special & in it's own conditional block because it should automatically be run on Escherichia and Shigella species; but optionally run on ANY bacterial sample if the user wants to screen for Shiga toxin genes + if (merlin_tag == "Escherichia" || merlin_tag == "Shigella sonnei" || call_stxtyper == true ) { + call stxtyper_task.stxtyper { + input: + assembly = assembly, + samplename = samplename, + docker = stxtyper_docker_image, + disk_size = stxtyper_disk_size, + cpu = stxtyper_cpu, + memory = stxtyper_memory, + enable_debugging = stxtyper_enable_debug + } + } if (merlin_tag == "Escherichia" || merlin_tag == "Shigella sonnei" ) { # tools specific to ALL Escherichia and Shigella species # @@ -755,6 +776,16 @@ workflow merlin_magic { File? virulencefinder_report_tsv = virulencefinder.virulencefinder_report_tsv String? virulencefinder_docker = virulencefinder.virulencefinder_docker String? virulencefinder_hits = virulencefinder.virulencefinder_hits + # stxtyper + File? stxtyper_report = stxtyper.stxtyper_report + String? stxtyper_docker = stxtyper.stxtyper_docker + String? stxtyper_version = stxtyper.stxtyper_version + Int? stxtyper_num_hits = stxtyper.stxtyper_num_hits + String? stxtyper_all_hits = stxtyper.stxtyper_all_hits + String? stxtyper_complete_operon_hits = stxtyper.stxtyper_complete_operon_hits + String? stxtyper_partial_hits = stxtyper.stxtyper_partial_hits + String? stxtyper_stx_frameshifts_or_internal_stop_hits = stxtyper.stxtyper_frameshifts_or_internal_stop_hits + String? stxtyper_novel_hits = stxtyper.stxtyper_novel_hits # Shigella sonnei Typing File? sonneityping_mykrobe_report_csv = sonneityping.sonneityping_mykrobe_report_csv File? sonneityping_mykrobe_report_json = sonneityping.sonneityping_mykrobe_report_json