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z?Q?U)t_|KenB4KYZO?tmKPrvV9?MMH6IjPv#ic=Lz#?I$9< zj>27jnMu^+|IqAR%SwLy#lo+dys>a;JoXaFUF|MS7iO%-n7UN$Uk+T?uGul-x3Y^C zgszEqEV>GuIeykUP>H2Y)`P9o z`)du4xP|k2Hp{*13CN}q9jPPwr}0iDNG_AY(*YmvUC2*{^qdaAsMW{k)U>1sm%lB=iofg{XGwcVxE2IVnn$N;h5*7))t^P^w!W2>>5wm~aL5M`_Sc92&Va;oAM zl&M#Zo3dI*jzx{e6|)n-U%Y-5o7|nPzx{l-r_$ry<>xe>+NZO&TGd#0cME^5XSb(o zr6{-N0-RoQizuFli+8A9D_zk`>U)*PD>S_(xjH*kI{7(q*t> cP;a@W!WV-E>a-8`E7dz(NPknYg$KU-e Date: Mon, 22 Jul 2019 10:52:44 -0400 Subject: [PATCH 2/4] fixed issue with import of test object --- tests/testthat/test_smaller.R | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/tests/testthat/test_smaller.R b/tests/testthat/test_smaller.R index 22552f9d..9eac337f 100644 --- a/tests/testthat/test_smaller.R +++ b/tests/testthat/test_smaller.R @@ -2,9 +2,8 @@ set.seed(1) -pbmc.file <- system.file('tests', 'testdata', 'smaller_pbmc_data.RDS', package = 'liger') -pbmc.file <- "~/liger/tests/testdata/smaller_pbmc_data.RDS" -load(pbmc.file) +pbmc.file <- "../testdata/smaller_pbmc_data.RDS" +pbmc.small <- readRDS(pbmc.file) # Tests for object creation #################################################################################### @@ -468,4 +467,4 @@ purity_2 = calcPurity(ligex, cluster_2) test_that("Returns correct purity", { expect_equal(purity_1, 1, tolerance = 1e-6) expect_equal(purity_2, 1, tolerance = 1e-6) -}) \ No newline at end of file +}) From 21bb39d3268d86e30696543d2eabf6f4b7430678 Mon Sep 17 00:00:00 2001 From: Joshua Sodicoff Date: Wed, 24 Jul 2019 10:50:26 -0400 Subject: [PATCH 3/4] tried to fix issue with test file loading again --- tests/testthat/test_smaller.R | 60 ++++++++++++++++++----------------- 1 file changed, 31 insertions(+), 29 deletions(-) diff --git a/tests/testthat/test_smaller.R b/tests/testthat/test_smaller.R index 22552f9d..87ca6648 100644 --- a/tests/testthat/test_smaller.R +++ b/tests/testthat/test_smaller.R @@ -3,7 +3,6 @@ set.seed(1) pbmc.file <- system.file('tests', 'testdata', 'smaller_pbmc_data.RDS', package = 'liger') -pbmc.file <- "~/liger/tests/testdata/smaller_pbmc_data.RDS" load(pbmc.file) # Tests for object creation @@ -55,31 +54,31 @@ rm(ligex.nofil, ligex.union, ligex.union.nofil) # Tests for data merging ########################################################################################## -#context('Sparse merging') +context('Sparse merging') # create fake datasets -#dataset1 <- as(matrix(0, nrow = 6, ncol = 5), 'CsparseMatrix') -#dataset1[c(1, 5, 14, 18, 21, 28)] <- 1:6 -#rownames(dataset1) <- paste0('gene', 11:16) -#colnames(dataset1) <- paste0('cell', 1:5) +dataset1 <- as(matrix(0, nrow = 6, ncol = 5), 'CsparseMatrix') +dataset1[c(1, 5, 14, 18, 21, 28)] <- 1:6 +rownames(dataset1) <- paste0('gene', 11:16) +colnames(dataset1) <- paste0('cell', 1:5) -#dataset2 <- as(matrix(0, nrow = 6, ncol = 6), 'CsparseMatrix') -#dataset2[c(3, 8, 12, 14, 20, 21, 35)] <- 1:7 -#rownames(dataset2) <- c(paste0('gene', 11:13), paste0('gene', 7:9)) -#colnames(dataset2) <- paste0('cell', 6:11) +dataset2 <- as(matrix(0, nrow = 6, ncol = 6), 'CsparseMatrix') +dataset2[c(3, 8, 12, 14, 20, 21, 35)] <- 1:7 +rownames(dataset2) <- c(paste0('gene', 11:13), paste0('gene', 7:9)) +colnames(dataset2) <- paste0('cell', 6:11) -#merged <- MergeSparseDataAll(list(dataset1, dataset2)) +merged <- MergeSparseDataAll(list(dataset1, dataset2)) -#test_that("Merged entries correct", { -# expect_equal(unname(merged[, 'cell2']), rep(0, 9)) -# expect_equal(unname(merged[, 'cell7']), c(0, 2, 0, 0, 0, 0, 0, 0, 3)) -# expect_equal(unname(merged['gene12', ]), c(0, 0, 3, 0, 0, 0, 2, 4, 5, 0, 0)) -# expect_equal(unname(merged['gene7', ]), rep(0, 11)) -# expect_equal(merged['gene13', 'cell9'], 6) -# expect_equal(merged['gene14', 'cell5'], 6) -#}) +test_that("Merged entries correct", { + expect_equal(unname(merged[, 'cell2']), rep(0, 9)) + expect_equal(unname(merged[, 'cell7']), c(0, 2, 0, 0, 0, 0, 0, 0, 3)) + expect_equal(unname(merged['gene12', ]), c(0, 0, 3, 0, 0, 0, 2, 4, 5, 0, 0)) + expect_equal(unname(merged['gene7', ]), rep(0, 11)) + expect_equal(merged['gene13', 'cell9'], 6) + expect_equal(merged['gene14', 'cell5'], 6) +}) -#rm(dataset1, dataset2, merged) +rm(dataset1, dataset2, merged) # Tests for normalization ########################################################################################## @@ -134,8 +133,8 @@ test_that("Number of genes is correct for intersection", { }) test_that("Gives warning when no genes selected", { - expect_warning(selectGenes(ligex, var.thresh = c(2.3, 2.3), do.plot = F, - combine = "intersection")) + expect_error(selectGenes(ligex, var.thresh = c(2.3, 2.3), do.plot = F, + combine = "intersection")) }) rm(ligex_higher, test_genes, gene_inclusion, gene_inclusion_higher, ligex_intersect) @@ -396,14 +395,15 @@ ligex@cell.data[["clusters"]] = ligex@alignment.clusters ligex_subset <- subsetLiger(ligex, clusters.use = c(1, 2, 3, 4)) test_that("Returns correct subsetted object", { expect_equal(names(ligex_subset@raw.data), c('tenx', 'seqwell')) - expect_equal(dim(ligex_subset@raw.data[[1]]), c(1359, 145)) - expect_equal(colnames(ligex_subset@raw.data[[1]])[1:3], c("GACCAAACGACTAC", - "TGATACCTCACTAG", "AGTTATGAACAGTC")) - expect_equal(dim(ligex_subset@raw.data[[2]]), c(2011, 133)) + expect_equal(dim(ligex_subset@raw.data[[1]]), c(10982, 119)) + expect_equal(colnames(ligex_subset@raw.data[[1]])[1:3], c("ATGCCAGACAGTCA", + "CCAAAGTGTGAGAA", "GACCAAACGACTAC")) + expect_equal(dim(ligex_subset@raw.data[[2]]), c(6705, 138)) expect_equal(colnames(ligex_subset@raw.data[[2]])[1:3], c("Myeloid_457", "Myeloid_671", "Myeloid_1040")) expect_equal(levels(ligex_subset@clusters), c("1", "2", "3", "4")) - expect_equal(nrow(ligex_subset@cell.data), 278) + expect_equal(nrow(ligex_subset@cell.data), 257) + expect_equal(rownames(ligex_subset@cell.data), rownames(ligex_subset@dr.coords["tsne"])) }) # TODO: Add tests for ligerToSeurat and seuratToLiger functions @@ -420,7 +420,7 @@ test_that("Returns correct Seurat object", { , tolerance = 1e-6) }) -ligex_from_seurat <- seuratToLiger(seurat_from_liger,combined.seurat = T, assays.use = c("RNA")) +ligex_from_seurat <- seuratToLiger(pbmc.seurat,combined.seurat = T, assays.use = c("RNA")) rm(seurat_from_liger, liger_from_seurat) @@ -468,4 +468,6 @@ purity_2 = calcPurity(ligex, cluster_2) test_that("Returns correct purity", { expect_equal(purity_1, 1, tolerance = 1e-6) expect_equal(purity_2, 1, tolerance = 1e-6) -}) \ No newline at end of file +}) + + From 74d1c7988ba01826c4b64a91ca978beea25bc59e Mon Sep 17 00:00:00 2001 From: Joshua Sodicoff <44811472+jsodicoff@users.noreply.github.com> Date: Wed, 24 Jul 2019 10:53:08 -0400 Subject: [PATCH 4/4] tried to fix issues with test object loading again --- tests/testthat/test_smaller.R | 71 ++++++++++++++++++----------------- 1 file changed, 37 insertions(+), 34 deletions(-) diff --git a/tests/testthat/test_smaller.R b/tests/testthat/test_smaller.R index 9eac337f..d283e871 100644 --- a/tests/testthat/test_smaller.R +++ b/tests/testthat/test_smaller.R @@ -2,8 +2,8 @@ set.seed(1) -pbmc.file <- "../testdata/smaller_pbmc_data.RDS" -pbmc.small <- readRDS(pbmc.file) +pbmc.file <- system.file('tests', 'testdata', 'smaller_pbmc_data.RDS', package = 'liger') +load(pbmc.file) # Tests for object creation #################################################################################### @@ -54,31 +54,31 @@ rm(ligex.nofil, ligex.union, ligex.union.nofil) # Tests for data merging ########################################################################################## -#context('Sparse merging') +context('Sparse merging') # create fake datasets -#dataset1 <- as(matrix(0, nrow = 6, ncol = 5), 'CsparseMatrix') -#dataset1[c(1, 5, 14, 18, 21, 28)] <- 1:6 -#rownames(dataset1) <- paste0('gene', 11:16) -#colnames(dataset1) <- paste0('cell', 1:5) +dataset1 <- as(matrix(0, nrow = 6, ncol = 5), 'CsparseMatrix') +dataset1[c(1, 5, 14, 18, 21, 28)] <- 1:6 +rownames(dataset1) <- paste0('gene', 11:16) +colnames(dataset1) <- paste0('cell', 1:5) -#dataset2 <- as(matrix(0, nrow = 6, ncol = 6), 'CsparseMatrix') -#dataset2[c(3, 8, 12, 14, 20, 21, 35)] <- 1:7 -#rownames(dataset2) <- c(paste0('gene', 11:13), paste0('gene', 7:9)) -#colnames(dataset2) <- paste0('cell', 6:11) +dataset2 <- as(matrix(0, nrow = 6, ncol = 6), 'CsparseMatrix') +dataset2[c(3, 8, 12, 14, 20, 21, 35)] <- 1:7 +rownames(dataset2) <- c(paste0('gene', 11:13), paste0('gene', 7:9)) +colnames(dataset2) <- paste0('cell', 6:11) -#merged <- MergeSparseDataAll(list(dataset1, dataset2)) +merged <- MergeSparseDataAll(list(dataset1, dataset2)) -#test_that("Merged entries correct", { -# expect_equal(unname(merged[, 'cell2']), rep(0, 9)) -# expect_equal(unname(merged[, 'cell7']), c(0, 2, 0, 0, 0, 0, 0, 0, 3)) -# expect_equal(unname(merged['gene12', ]), c(0, 0, 3, 0, 0, 0, 2, 4, 5, 0, 0)) -# expect_equal(unname(merged['gene7', ]), rep(0, 11)) -# expect_equal(merged['gene13', 'cell9'], 6) -# expect_equal(merged['gene14', 'cell5'], 6) -#}) +test_that("Merged entries correct", { + expect_equal(unname(merged[, 'cell2']), rep(0, 9)) + expect_equal(unname(merged[, 'cell7']), c(0, 2, 0, 0, 0, 0, 0, 0, 3)) + expect_equal(unname(merged['gene12', ]), c(0, 0, 3, 0, 0, 0, 2, 4, 5, 0, 0)) + expect_equal(unname(merged['gene7', ]), rep(0, 11)) + expect_equal(merged['gene13', 'cell9'], 6) + expect_equal(merged['gene14', 'cell5'], 6) +}) -#rm(dataset1, dataset2, merged) +rm(dataset1, dataset2, merged) # Tests for normalization ########################################################################################## @@ -133,8 +133,8 @@ test_that("Number of genes is correct for intersection", { }) test_that("Gives warning when no genes selected", { - expect_warning(selectGenes(ligex, var.thresh = c(2.3, 2.3), do.plot = F, - combine = "intersection")) + expect_error(selectGenes(ligex, var.thresh = c(2.3, 2.3), do.plot = F, + combine = "intersection")) }) rm(ligex_higher, test_genes, gene_inclusion, gene_inclusion_higher, ligex_intersect) @@ -253,7 +253,7 @@ test_that("Alignment and clustering are correct", { test_that("SNF is correct", { expect_equal(dim(as.data.frame(ligex@snf[["idents"]])), c(494,1)) expect_equal(rownames(as.data.frame(ligex@snf[["idents"]]))[1:5], c("ATGCCAGACAGTCA", - "CCAAAGTGTGAGAA", "GACCAAACGACTAC", "TGATACCTCACTAG", "AGTTATGAACAGTC")) + "CCAAAGTGTGAGAA", "GACCAAACGACTAC", "TGATACCTCACTAG", "AGTTATGAACAGTC")) expect_equal(dim(ligex@snf[["out.summary"]]), c(98800,3)) expect_equal(ligex@snf[["out.summary"]][1563,], c(7.0, 178.0, 0.1)) expect_equal(ligex@snf[["out.summary"]][56891,], c(284, 156, 0.35)) @@ -316,13 +316,13 @@ test_that("Dimensions are correct", { expect_equal(ligex@dr.coords[["tsne"]][56:60], c(-4.00397980, -0.24390106, -4.52338154, 0.20838293, 0.02052938), tolerance = 1e-6) expect_equal(as.vector(ligex@dr.coords[["tsne"]][56:60,2]), c(3.955963, 6.510291, 1.385297, - 4.285448, 4.960550), tolerance = 1e-6) + 4.285448, 4.960550), tolerance = 1e-6) expect_equal(dim(ligex_rawtsne@dr.coords[["tsne"]]), c(494, 2)) expect_equal(ligex_rawtsne@dr.coords[["tsne"]][312:316], c(-9.027826, -4.045090, -1.801967, - -2.801441, 7.784805), tolerance = 1e-6) + -2.801441, 7.784805), tolerance = 1e-6) expect_equal(as.vector(ligex_rawtsne@dr.coords[["tsne"]][312:316,2]), c(-12.375348, -7.008916, - -5.619409, -5.278337, 9.237971), tolerance = 1e-6) + -5.619409, -5.278337, 9.237971), tolerance = 1e-6) }) @@ -395,14 +395,15 @@ ligex@cell.data[["clusters"]] = ligex@alignment.clusters ligex_subset <- subsetLiger(ligex, clusters.use = c(1, 2, 3, 4)) test_that("Returns correct subsetted object", { expect_equal(names(ligex_subset@raw.data), c('tenx', 'seqwell')) - expect_equal(dim(ligex_subset@raw.data[[1]]), c(1359, 145)) - expect_equal(colnames(ligex_subset@raw.data[[1]])[1:3], c("GACCAAACGACTAC", - "TGATACCTCACTAG", "AGTTATGAACAGTC")) - expect_equal(dim(ligex_subset@raw.data[[2]]), c(2011, 133)) + expect_equal(dim(ligex_subset@raw.data[[1]]), c(10982, 119)) + expect_equal(colnames(ligex_subset@raw.data[[1]])[1:3], c("ATGCCAGACAGTCA", + "CCAAAGTGTGAGAA", "GACCAAACGACTAC")) + expect_equal(dim(ligex_subset@raw.data[[2]]), c(6705, 138)) expect_equal(colnames(ligex_subset@raw.data[[2]])[1:3], c("Myeloid_457", "Myeloid_671", "Myeloid_1040")) expect_equal(levels(ligex_subset@clusters), c("1", "2", "3", "4")) - expect_equal(nrow(ligex_subset@cell.data), 278) + expect_equal(nrow(ligex_subset@cell.data), 257) + expect_equal(rownames(ligex_subset@cell.data), rownames(ligex_subset@dr.coords["tsne"])) }) # TODO: Add tests for ligerToSeurat and seuratToLiger functions @@ -419,7 +420,7 @@ test_that("Returns correct Seurat object", { , tolerance = 1e-6) }) -ligex_from_seurat <- seuratToLiger(seurat_from_liger,combined.seurat = T, assays.use = c("RNA")) +ligex_from_seurat <- seuratToLiger(pbmc.seurat,combined.seurat = T, assays.use = c("RNA")) rm(seurat_from_liger, liger_from_seurat) @@ -434,7 +435,7 @@ test_that("Returns correct dataset specificity", { expect_equal(dataset_spec[[2]][6:10], c(100.91134, 112.07151, 123.82733, 77.75074, 88.71075), tolerance = 1e-6) expect_equal(dataset_spec[[3]][11:15], c(0.03951941, -0.51374525, -0.70291549, -0.06761655, - -0.79589711),tolerance = 1e-6) + -0.79589711),tolerance = 1e-6) }) #calcAgreement @@ -468,3 +469,5 @@ test_that("Returns correct purity", { expect_equal(purity_1, 1, tolerance = 1e-6) expect_equal(purity_2, 1, tolerance = 1e-6) }) + +