Release 3.0.6
This release has several improvements:
-
RelionCor supports dose weighting of datasets at 100 kV.
-
The inter-particle distance cutoff in the Laplacian-of-Gaussian (LoG) picker was improved.
When LoG picker is enabled,minimum inter-particle distance
in the "autopicking" tab is not used. This is by design. Instead, neighbouring particles within
(detected diameter at the point +--LoG_diam_min
) *--LoG_neighbour
/ 100
are avoided. The default for--LoG_neighbour
is 100 %. You can make this smaller or larger to pick more or less, respectively. -
Implemented
--LoG_upper_threshold
option.
LoG picker tended to pick high contrast contamination like ice and ethane droplets. They have higher scores than real particles, soadjust default threshold
is useless. Now we have--LoG_upper_threshold
option to set an upper limit in scores. Useful values are typically 1.5 to 4. The default is 99999 (i.e. no limit in practice)
This release also contains the following bug fixes.
- PostProcess: fixed a bug where
postprocess_masked.mrc
is overwritten byhalf2_masked.mrc
when--half_maps
is provided (reported by @keitaroyam). - Stability of histogram generation was improved.
- Prevented "too long arguments" errors in scratch (issue #467 reported by @EMBL-Structures).
relion_star_handler --split
: removed an assumption on the number of digits.- AutoPicker: rejects 3D references for filaments (CCPEM https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind1906&L=CCPEM&O=D&P=6094).