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# ePlant | ||
ePlant | ||
ePlant is a gene-centered visualization tool for plant genomes. | ||
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## Using ePlant | ||
Create a new tab or choose a gene of interest (GOI) using the search bar on the left. | ||
> Currently the only plant genome available is that of _Arabidopsis thaliania_. | ||
Use the navigation dropdown to discover more information about the expression of that gene from a phenotypic to a molecular level. | ||
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Currently available views: | ||
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[ℹ️ Gene information]() | ||
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[📑 Publications ]() | ||
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[🌱 Plant]() | ||
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[🔬 Tissue & Experiment]() | ||
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> Coming views: | ||
🌡️ Heat map | ||
🌎 World | ||
🧬 Chromosome | ||
Interactions | ||
Molecule | ||
Sequence | ||
🔄 Pathways | ||
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### Gene information | ||
Contains a list of gene information: | ||
- Which views are available for this gene | ||
- The gene name and aliases | ||
- Brief and computational descriptions | ||
- Curator summary | ||
- Location and gene model | ||
- DNA sequence | ||
- Protein sequence | ||
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### Publication | ||
A list of relevant publications. | ||
- Author, year, journal, title, and links to publications associated with the GOI | ||
- Gene RIFs with annotations and links source to paper | ||
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### Plant (cm scale) | ||
A visual map of where the gene is expressed in the body of the plant. | ||
- Choose a data mode (relative/absolute) | ||
- Select diagram origin: [AtGenExpress](https://www.arabidopsis.org/portals/expression/microarray/ATGenExpress.jsp) or Klepikova (for Anna Klepikova, author of [this paper](https://pubmed.ncbi.nlm.nih.gov/27549386/) on arabidopsis) | ||
- Sort diagrams by name or expression level | ||
- Hover over images for more information | ||
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### Tissue & Experiment (mm scale) | ||
Expression patterns in different cells. | ||
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<!-- | ||
### Heat map | ||
Based on experiments done by the lab, the striated bars show [when?where?] a given gene is expressed. | ||
Yellow: weak, red: strong | ||
### World (km scale) | ||
Gene extression infomration plotted on a map. | ||
You can overlay climate information (precipitation, historical max temp, historical min temp). | ||
### Chromosome (micro-meter, sub-micrometer) | ||
Localization of the gene on the chromosomes. Can see local genes as well | ||
### Interaction level | ||
Protein-protein and protein-DNA interactions for a given gene and geen product. | ||
### Molecule (nano meter) | ||
Protein sequence | ||
### Sequence viewer | ||
JBrowser instance from Araport | ||
### Navigation | ||
See the relations of the gene to other plant genes. --> | ||
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## Roadmap | ||
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## How to contribute | ||
Read the [onboarding blueprint](https://github.com/BioAnalyticResource/ePlant/issues/29). | ||
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If you'd like to contribute, [pick an issue](https://github.com/BioAnalyticResource/ePlant/issues). We use a forking workflow. For an in-depth look at what that means, [read this article](https://www.atlassian.com/git/tutorials/comparing-workflows/forking-workflow). | ||
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### Quickstart | ||
1. Create a fork and download it to your local machine. | ||
```bash | ||
git clone <your fork address> | ||
``` | ||
1. Set up the main ePlant project as your upstream branch. | ||
```bash | ||
git remote add upstream https://github.com/BioAnalyticResource/ePlant | ||
``` | ||
1. Verify your node and npm versions. Most folks are working with Node v18.0.0^ and npm 9.8.0^. | ||
```bash | ||
node -v | ||
npm -v | ||
``` | ||
If that command doesn't return anything, [install the LTS version of Node](https://nodejs.org/en/download). | ||
1. Download all dependencies. | ||
```bash | ||
npm i | ||
``` | ||
1. Run your local version of ePlant. | ||
```bash | ||
npm run dev | ||
``` | ||
1. Comment on a ticket to take it. | ||
1. Turn on notifications for that thread. | ||
1. Complete the necessary fix. | ||
1. Push your changes to **your** fork. | ||
```bash | ||
git push origin main | ||
``` | ||
1. Create a pull request. | ||
> Underneath where it says **Compare changes**, there is a line that says | ||
> `Compare changes across branches, commits, tags, and more below. If you need to, you can also compare across forks.` | ||
Click that link to bring up a dropdown menu of ePlant forks. | ||
1. Choose your fork as the head repository and `https://github.com/BioAnalyticResource/ePlant/` as the base repository. | ||
1. Give the pull request a descriptive title so that someone who didn't work on it will know what you did. Link the issue if possible. | ||
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### Reviewing a pull request | ||
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## Glossary | ||
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**AtGenExpress**: A multinational effort designed to uncover the transcriptome of the multicellular model organism _Arabidopsis thaliana_. | ||
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**BAR**: Bio-analytic resource; a group of bioinformatics tools created by the [lab of Dr. Nicholas Provart](http://provart.csb.utoronto.ca/the-lab/) at the University of Toronto. | ||
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**BAR API**: An application programming interface of bio-analytic resources available at https://bar.utoronto.ca/api/. | ||
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**GOI**: Gene of interest; the gene displayed in ePlant. | ||
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**eFP**: Electronic fluorescent pictographic | ||
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**GeneRIF**: Gene reference into function; A [standard](https://www.ncbi.nlm.nih.gov/gene/about-generif) that provides a mechanism to allow scientists to add to the functional annotation of genes described in [Gene](https://www.ncbi.nlm.nih.gov/gene). |